Rapid and affordable generation of compensation beads for nanoscale flow cytometry

Abstract With the introduction of dedicated nanoscale flow cytometers, the need for suitable compensation beads has emerged. Here, we present a rapid and cost-effective method to generate ∼100 nm antibody-binding compensation beads compatible with a wide range of antibody species for use in nanoscale flow cytometry. This approach may provide a practical interim solution until commercial alternatives become available. Competing Interest Statement The authors have declared no competing interest. Funder Information Declared Carlsberg Foundation, https://ror.org/01kpjmx04, CF23-1020 Copyright The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.