The gene expression fluctuations of glyceraldehyde-3-phosphate dehydrogenase in drought-stressed-basil cultivars

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), as one of the key enzymes in glycolytic pathway in higher plants, was identified as an important objective protein in oxidative stresses condition, which can undergo thiolation reaction in the active site. However, several genes encoding of cytosolic isoenzymes of GAPDH have been identified and characterized in some plants, to investigate the role of GAPDH under drought stress, cloning of full-length cDNA, and expression profile of Ocimum basilicum GAPDH ( ObGAPDH ) cDNA were investigated. The full-length ObGAPDH was 1315 bp long, its deduced protein consists of 399 aminoacids with a predicted molecular mass of 42.54 kDa and a pI of 6.01. Phylogenetic tree study exhibited a ObGAPDH shared evolutionary relationship with the protein sequence of ObGAPDH reported in Salvia splendens and Sesamum indicum . In silico identified oxidative function of ObGAPDH in response to stimuli and docking illustrated inaction of protein with H 2 O 2 as ligand. Expression profiling analysis exhibited different transcription ratios of ObGAPDH gene under water shortage stress. Overall, our findings revealed that the ObGAPDH regulation appears to be a cultivar- and drought stress-dependent mechanism.