Isolation and exploration of potential probiotic attributes of a novel isolate Pedicoccus acidilactici CITKHZ7 obtained from traditional fermented fish paste Hentak

In vitro probiotic and antibacterial activity was demonstrated by a novel isolate CITKHZ7 identified as Pediococcus acidilactici . It exhibited adequate resistance against the severe circumstance of gastrointestinal tract (GI), surviving at low acidic pH (pH 2.0) for 5hr. The initial viable cell population of isolate CITKHZ7 was reduced by 2.84% and 4.48% by artificial gastric juice and simulated intestinal fluid respectively. Enzyme lysozyme (100 mg/ml) and bile salt (0.3%) both reduced vitality by 13.92% and 10.35% within 5hr. The isolate did not show any significant bile salt deconjugation activity. The cell surface hydrophobicity of 45.94% was highest against hydrocarbon chloroform and the isolate cell surface was seen to be electrophilic in nature. In this research study, 51% autoaggregation was observed in the isolate CITKHZ7 within 5hr. Both of these properties are required for attachment to epithelial cells and colonisation of the host. The in-vitro adhesion of the CITKHZ7 was carried out with human epithelial carcinoma cell line Caco-2.