The effect of cyclical stimulation on the primary neurons of Bombyx Mori

Changes to the Silkworm axon initiation segment in frequently firing neurons are known to be important predictors of silk production in the Japanese Silkworm ( Bombyx Mori ). Studying this phenomenon in primary neurons is critical to understanding the regulation of silk production. To study these changes we used cyclical stimulations using micro-electrodes to the Silkworm axon initiation segment of primary neurons. Numerical simulation results indicate that Silkworm axon initiation segment water potential fluctuated sinusoidally on high voltage only. Fluctuations in the amplitude and trend were caused by calcium flow and storage resistance, respectively. The change in Silkworm axon initiation segment-stored water was proportional to the change rate in water potential. Silkworm axon initiation segment diameter increased with fluctuations in calcium free media; moreover, it varied slightly under low voltage conditions. Our results indicate that the changes in Silkworm axon initiation segment diameter were caused by changes in water potential, which can be attributed to subcellular gated calcium channels within the primary neurons.