EVALUATION OF THE FREQUENCY OF THE G-765C POLYMORPHISM IN THE PROMOTER REGION OF THE COX2 GENE IN WOMEN WITH ENDOMETRIOSIS

We aimed to evaluate the frequency of the G-765C/rs20417 polymorphism in theCOX2gene and the gene expression in the endometrium of women with endometriosis. We performed a casecontrol study comprising 257 infertile and30 fertile women diagnosed with endometriosis, and 302 fertile women as controls. Considering theCOX2in the endometrium, we considered 28 infertile women with endometriosis and 19controls. Genotyping was performed using the HRM methodology by PCR real-time. The expression ofGAPDHandCOX2was measured using the qRTPCR methodology. The genotype and allele distribution was similar in both infertile (p=0.718; 95% CI, 0.69–1.27) and fertile women (p=0.054; 95% CI, 1.03–3.36) with endometriosis and in the control group. The genotype distribution according to the stage of the disease (I/II and III/IV) were similar (p=0.729; 95% CI, 0.75–1.57); (p=0,920; 95% CI, 0.68-1.37), respectively. For the expression of the COX2 gene, the results are in mean value and standard deviation 31.34 (30.28–32.40); 32.31 (31.40–33.22) respectively, showing no statistical significance (p=0.161). The presence of G-765C/rs20417polymorphism in the COX2 gene is not associated with either the presence or the stage of endometriosis. The expression in the endometrium did not differ between the case and control groups, as well as the stage of endometriosis. This study was supported by FAPESP (Fundacao de Amparo a Pesquisa do Estado de SaoPaulo); #2012/03564-0.