Group 6 LEA proteins are key players in tolerance to water deficit, and in maintaining the glassy state and longevity of seeds

Group 6 LEA proteins are key players in tolerance to water deficit, and in maintaining the glassy state and longevity of seeds | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Plant, Cell & Environment This is a preprint and has not been peer reviewed. Data may be preliminary. 29 January 2025 V1 Latest version Share on Group 6 LEA proteins are key players in tolerance to water deficit, and in maintaining the glassy state and longevity of seeds Authors : Inti A. Arroyo-Mosso , H. Nicholay Diaz-Ardila , Alejandro Garciarrubio 0000-0002-4924-2823 , U. G. V. S. S. Kumara 0009-0005-6034-8580 , David F. Rendón-Luna 0000-0003-3804-9988 , Teresa B. Nava-Ramírez , Thomas C. Boothby , Jose Reyes L , and Alejandra A. Covarrubias 0000-0003-0439-3414 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.173813265.58260174/v1 480 views 94 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Plants have a wide range of adaptive and protective mechanisms to cope with dehydration. Central in these processes are the Late Embryogenesis Abundant (LEA) proteins, whose levels notably increase in response to dehydration during seed development and vegetative tissues. Understanding the function of LEA proteins is essential for gaining insights into plant development and their adaptive responses to environmental stress. This study focuses on group 6 LEA proteins (LEA6) from Arabidopsis thaliana : AtLEA6-2.1, AtLEA6-2.2, and AtLEA6-2.3. Phylogenetic analysis reveals that LEA6 family emerged with seed plants, pointing to a unique role in seed viability. Functional characterization using T-DNA insertion mutants demonstrated that AtLEA6-2.1, but not AtLEA6-2.2, is essential for tolerance to high-osmolarity and salinity during germination and post-germination growth. AtLEA6-2.1 deficiency also altered root architecture under salinity, increasing primary root length while reducing lateral root number and length, suggesting a role in root development not described before for a LEA protein. Furthermore, AtLEA6-2.1 is critical for seed longevity, as mutants lacking this protein showed reduced germination after natural and accelerated aging. These mutants exhibited increased glass-former fragility, indicating that AtLEA6-2.1 deficiency reduces cellular viscosity, which we found correlates with reduced longevity. Our investigation extends to protective protein assays under dehydration, revealing that the acidic nature of this protein family requires specific conditions for its in vitro protective activity. Overall, this study underscores the essential role of AtLEA6-2.1 in the plant response to low-water availability, seed longevity, and glassy state properties, making it a potential target for enhancing plant resilience to environmental challenges. Supplementary Material File (arroyo mosso et al_pce.docx) Download 6.37 MB File (arroyo-mosso et al_raw figure images.pdf) Download 3.60 MB File (figure 5_arroyo mosso et al.pdf) Download 919.88 KB File (figure 7_arroyo mosso et al.pdf) Download 750.90 KB Information & Authors Information Version history V1 Version 1 29 January 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Collection Plant, Cell & Environment Keywords intrinsically disordered proteins lea proteins seed longevity water deficit water relations Authors Affiliations Inti A. Arroyo-Mosso Universidad Nacional Autonoma de Mexico Instituto de Biotecnologia View all articles by this author H. Nicholay Diaz-Ardila Universidad Nacional Autonoma de Mexico Instituto de Biotecnologia View all articles by this author Alejandro Garciarrubio 0000-0002-4924-2823 Universidad Nacional Autonoma de Mexico Instituto de Biotecnologia View all articles by this author U. G. V. S. S. Kumara 0009-0005-6034-8580 University of Wyoming Department of Molecular Biology View all articles by this author David F. Rendón-Luna 0000-0003-3804-9988 Universidad Nacional Autonoma de Mexico Instituto de Biotecnologia View all articles by this author Teresa B. Nava-Ramírez Universidad Nacional Autonoma de Mexico Instituto de Biotecnologia View all articles by this author Thomas C. Boothby University of Wyoming Department of Molecular Biology View all articles by this author Jose Reyes L Universidad Nacional Autonoma de Mexico Instituto de Biotecnologia View all articles by this author Alejandra A. Covarrubias 0000-0003-0439-3414 [email protected] Universidad Nacional Autonoma de Mexico Instituto de Biotecnologia View all articles by this author Metrics & Citations Metrics Article Usage 480 views 94 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Inti A. Arroyo-Mosso, H. Nicholay Diaz-Ardila, Alejandro Garciarrubio, et al. Group 6 LEA proteins are key players in tolerance to water deficit, and in maintaining the glassy state and longevity of seeds. Authorea . 29 January 2025. DOI: https://doi.org/10.22541/au.173813265.58260174/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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