Biopsy and Interventional Therapy of Subglottic Lesions with Flexible Bronchoscope under Protection of Endotracheal Intubation

Biopsy and Interventional Therapy of Subglottic Lesions with Flexible Bronchoscope under Protection of Endotracheal Intubation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Biopsy and Interventional Therapy of Subglottic Lesions with Flexible Bronchoscope under Protection of Endotracheal Intubation huihui Lin, Tingfen Ji, Hao Zheng, Wenjiang Ma, Shengwen song, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5050685/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 28 Apr, 2025 Read the published version in European Journal of Medical Research → Version 1 posted 7 You are reading this latest preprint version Abstract Subglottic lesions represent a complex and challenging clinical entity, often associated with high procedural risks due to their anatomical location and vascularity. While a laryngeal mask airway(LMA) is commonly employed for airway management in such cases, it has notable limitations, particularly in scenarios involving significant bleeding or the need for extended intervention. This article presents an alternative and effective approach for the biopsy and interventional treatment of subglottic lesions using a flexible bronchoscope under the protection of an endotracheal tube (ETT). By inserting the bronchoscope through the space between the ETT and the tracheal wall, various procedures—including biopsy, argon plasma coagulation (APC), and CO₂ cryotherapy—can be performed with relative safety. This method provides a feasible option for airway protection, bleeding control, and lesion management, offering new possibilities in clinical practice. Bronchoscopy Subglottic Lesions Endotracheal Intubation Laryngeal mask airway Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Introduction Various benign and malignant airway diseases, especially central airway diseases, can result in various clinical symptoms, including coughing, wheezing, dyspnea, and recurrent airway infections distal to the lesion [ 1 ] . In addition, central airway diseases are usually rich in blood supply and can cause fatal bleeding when the lesion ruptures. Without timely intervention, asphyxia and even death can occur at any time [ 2 ] . Therefore, central airway diseases require early intervention. The rigid bronchoscope has a large orifice for easy operation while maintaining airway patency and respiratory support [ 2 ] . If there is heavy bleeding during the operation, a large-aperture suction tube can be used to ensure a clear field of view while argon plasma coagulation (APC), high-frequency electric knife (HFEK), and laser photocoagulation are utilized to stop the bleeding. Additionally, the sharp angle at the tip of the hard metal bronchoscope tube allows for direct "removal" of the tumor under the bronchoscope [ 3 ] . Therefore, rigid bronchoscopy is often the first choice for treating central airway diseases [ 4 ] . However, the subglottic lesion poses a challenge to rigid bronchoscopy due to the restricted field of view. When dealing with subglottic lesions, a laryngeal mask airway (LMA) is usually used for airway management. The LMA facilitates the insertion of the flexible bronchoscope at the level of the glottic opening and provides excellent visualization of the anatomy and dynamics of the glottis, subglottic area, and other parts of the airway. It also maintains airway patency during general anesthesia, permitting spontaneous respiration and allowing for assisted ventilation if necessary [ 5 ] . Therefore, an LMA combined with a flexible bronchoscope is the most common choice for these lesions [ 6 ] . But the use of LMA has some shortcomings. When the blood supply of the lesion is abundant and intraoperative bleeding occurs, it is hard to control the bleeding and ensure adequate ventilation with an LMA [ 7 ] . Although the rapidly developing vascular interventional techniques in recent years can effectively reduce the risk of bleeding by embolizing the lesion vessels to achieve preoperative prevention or intraoperative hemostasis, there will always be various circumstances that limit its application [ 8 ] . For example, the onset of subglottic lesion is often ferocious. Patients tend to visit grass-roots hospitals, and it is difficult for them to tolerate long-distance transport. In addition, the medical unit lacks the means to perform bronchial artery embolization, the condition is too urgent to allow for vascular intervention, or the patient has an iodine allergy. This paper will report a safe and effective method for biopsy or interventional therapy via a flexible bronchoscope under the protection of endotracheal intubation. A flexible bronchoscope was placed in the gap between the endotracheal tube (ETT) and the trachea. Biopsy forceps, APC, CO 2 cryotherapy, and other equipment were used to perform operations such as biopsy and tumor removal through the working orifice of the flexible bronchoscope. Balloon incarceration in the distal airway of the lesion can effectively prevent the mass from falling off and the risk of suffocation caused by severe bleeding ( Fig. 1 ) . Methods and Results Patient 1 A 45-year-old female with an 8-day history of hemoptysis was admitted to the respiratory department on October 12, 2019. She had a history of thyroidectomy six years prior and was known to be allergic to iodinated contrast media. Chest computed tomography (CT) revealed a mural nodule in the upper trachea, approximately 1 cm below the glottis. Bronchoscopy identified a subglottic nodular bulge arising from the airway mucosa, which appeared hypervascular (Fig. 2 A). Patient 2 A 62-year-old male was admitted to the respiratory department on August 02, 2021, with a 40-days history of intermittent dyspnea. His medical history included radical surgery and chemoradiotherapy for small cell lung cancer three years earlier, as well as rigid endotracheal tumor resection performed one year prior to admission. One month before admission, the patient experienced a sudden episode of coma lasting about one hour and underwent emergency tracheal intubation. Following the procedure, he developed recurrent hemoptysis. Bronchoscopy performed in July 2021 revealed a subglottic mucosal bulge (Fig. 2 B). Based on chest CT conducted two weeks prior to symptom onset, upper airway tumor invasion was suspected. It was presumed that the majority of the tumor tissue had been dislodged during the intubation process. Preoperative Consideration In both cases, a thorough preoperative evaluation was conducted to determine the most appropriate interventional strategy. Several commonly used approaches for the management of subglottic lesions were carefully assessed. The risks and feasibility of each method were thoroughly explained to the patients, and informed consent was obtained prior to the procedure. Patient 1 had a known allergy to iodinated contrast media, which made vascular embolization infeasible. She also declined awake intubation due to anxiety and poor tolerance. Rigid bronchoscopy was considered but abandoned because the lesion was located only 1 cm below the glottis, leaving insufficient operating space for the rigid scope tip. LMA, though commonly used in such procedures, was also deemed unsuitable due to the rich vascularity of the lesion and the high risk of intraoperative bleeding that would be difficult to manage via LMA ventilation. Patient 2 had a complex medical and surgical history, including radical resection and radiotherapy for small cell lung cancer three years earlier and rigid bronchoscopic tumor resection one year earlier. Due to the proximity of the lesion to the acoustic hilum, rigid bronchoscopy was deemed unsuitable due to limited surgical space. In addition, the anesthesiologist was concerned that positive pressure ventilation through the anesthesia machine might be compromised, which could lead to inadequate oxygenation. Intraoperative bleeding is also difficult to control with LMA-assisted ventilation. Both patients had subglottic lesions. Finally, we chose to apply flexible bronchoscopy under the protection of tracheal intubation for biopsy sampling in patient 1 and biopsy sampling and partial removal of tumor tissue in patient 2. Anesthesia To reduce the stress response during intubation, lidocaine cream was applied to lubricate the ETT. General anesthesia was induced with intravenous administration of etomidate, alfentanil, and atracurium. Anesthesia was maintained using a combination of propofol, remifentanil, and sevoflurane. Epinephrine was prepared in advance as a rescue medication in case of emergency. ETT 4.5 (Covidien, Origin: Shanghai, Model: 18860, OD 6.6mm, ID 4.5mm) was selected, and tracheal intubation was performed under the guidance of a flexible bronchoscope. Under direct vision, the ETT was seen to cross the distal end of the lesion by approximately 2–3 cm. The balloon was then inflated until the trachea was incarcerated (Fig. 3 ). The proximal end of the ETT is connected to a ventilator to maintain breathing; Biopsy and Interventional Therapy The flexible bronchoscope (Olympus, OD 5.5mm, ID 2.8mm) was placed in the gap between the ETT and the trachea. Various treatments can be performed through the working orifice of the flexible bronchoscope. Balloon incarceration in the distal airway of the lesion can effectively prevent the mass from falling off and the risk of suffocation caused by severe bleeding ( Fig. 4 A-D ) . In patient 1, the bleeding volume after the biopsy was about 90 ml, which was stopped by 1:1000 epinephrine plus 4°C ice physiological saline and APC. During the operation, the patient had stable oxygenation and blood pressure and no significant bleeding from the distal airway after removing the tracheal intubation balloon. The operation time was near 32 minutes. In patient 2, In the second case, the bleeding volume was about 10 ml after the biopsy, followed by CO 2 cryotherapy and APC to eliminate part of the tumor tissue. The operation time was 45 minutes. Discussion In clinical practice, biopsy or intervention for the subglottic lesion can be a high-risk and challenging task. As far as we know, we are the first to report this method, which is relatively safe, practical, feasible, and easy to apply. Undoubtedly, the most significant disadvantage of this method is that the ETT occupies airway space, which limits bronchoscopy, while the limited operating space might make it difficult to use thermal therapy such as laser therapy or APC near flammable tubes. Therefore, we recommend choosing an ETT with an ID of 4.5 mm to reserve space for subsequent surgery, which can avoid the exposure of thermal therapy to the flammable ETT as much as possible. Additionally, microlaryngoscopy tubes (MLTs), which are specifically designed for airway procedures in narrow spaces, may serve as a safer and more standardized alternative to conventional ETTs. Their potential advantages in minimizing space occupation and improving intraoperative safety warrant further exploration in future practice [ 9 ] . In fact, including the above two cases, we have also used this approach to remove granulation by laser therapy in a patient who developed a stricture above the incision with a post-tracheostomy (Fig. 5 A). Previous experience showed that the patient had severe subglottic capillary leakage and that the lesion was prone to bleeding when touched. It is worth noting that, clinically, when faced with this situation, silicone stents were mostly chosen to be placed in the airway. However, severe granulation tissue growth can still occur after stent placement [ 10 ] . Therefore, we first performed balloon dilation (diameter 15mm) at the airway stenosis and then treated the lesion with laser therapy using the method described above. Remarkably, the procedure went very well, and the airway recovered well at the 6-month follow-up that has continued since the surgery ( Fig. 5 B ) . Subglottic stenosis remains a particularly challenging condition due to the narrow anatomical structure and risk of airway compromise. Di Felice et al.'s retrospective comparison of rigid bronchoscopy versus flexible bronchoscopy under general anesthesia found no significant differences in outcomes [ 11 ] . Although rigid bronchoscopy offers advantages in instrument capacity, the widespread availability and relative simplicity of balloon catheters delivered through flexible bronchoscopes make this approach more accessible, particularly for operators without specialized training in rigid techniques [ 12 ] . When encountering subglottic masses, several approaches are commonly considered, including rigid bronchoscopy, awake fiberoptic bronchoscopy, bronchial artery embolization, and flexible bronchoscopy with LMA. Among these, awake fiberoptic bronchoscopy is considered one of the safest options as it preserves spontaneous breathing and patient awareness. However, in therapeutic procedures that require prolonged operation time, patients may experience significant discomfort, and lack of cooperation can increase the risk of airway injury. Additionally, Chang et al. conducted a study on 49 patients with laryngeal lesions who underwent carbon dioxide laser surgery. They found that while there was significant improvement in voice function for patients with benign lesions and Reinke's edema, limitations in tolerance and therapeutic efficacy were observed [ 13 ] . This may be analogous to awake fiberoptic bronchoscopy, which could be a safer choice for lesions with shorter expected procedural times, provided the patient has a high tolerance. Notably, for patients with anticipated difficult airways, awake fiberoptic bronchoscopy via the oral route may also be a viable option [ 14 ] . Rigid bronchoscopy offers a wide operating channel and relatively safe airway control, but it is often unsuitable for subglottic lesions due to limited accessibility and may cause mechanical trauma to the airway. LMA-assisted bronchoscopy maintains ventilation and offers procedural flexibility but is not ideal for highly vascular lesions, where bleeding is difficult to control through LMA ventilation alone. For hypervascular tumors, bronchial artery embolization may be effective in reducing intraoperative bleeding. However, this technique does not provide histological diagnosis or immediate lesion removal. In addition, embolization is contraindicated in patients with contrast agent allergies, as was the case in Patient 1. Comparison of Different Management Techniques for Subglottic Lesions shown in Table 1 . Table 1 Comparison of Different Management Techniques for Subglottic Lesions Technique Advantages Disadvantages Flexible bronchoscope under ETT Protection - Good visualization of the subglottic area - Balloon tamponade enables bleeding control - Minimizes risk of tumor dislodgement - Limited operative space - Requires experienced bronchoscopist and anesthesiologist - Requires tolerance to general anesthesia Flexible bronchoscope with LMA - Preserves spontaneous respiration - Suitable for short diagnostic procedures and therapeutic interventions - Ineffective bleeding control Rigid bronchoscope - Wide working channel - Suitable for resection of large or hemorrhagic lesions - Limited visualization of subglottic area - Higher risk of airway trauma Interventional embolization - Minimally invasive - Effective for hypervascular lesions - Contraindicated in patients with iodine allergy - Not allow biopsy or lesion resection Awake fiberoptic intubation - Contraindicated in patients with iodine allergy - Does not allow biopsy or lesion resection - Poor patient comfort - Requires patient cooperation Potential complications of our method include airway trauma due to repeated scope manipulation, thermal injury from the use of APC or laser therapy near the ETT, and anesthesia-related events such as hypotension or delayed recovery. To minimize these risks, we emphasize the importance of careful patient selection and continuous intraoperative monitoring of hemodynamic parameters. The procedure should be performed by a team experienced in both bronchoscopy and airway management. In addition, airway management plays a critical role in this patient population. In both cases presented, oxygen saturation remained stable above 90%, end-tidal CO₂ (EtCO₂) levels were within the normal range (35–45 mmHg), and peak airway pressure did not exceed 30cmH₂O. These parameters indicate that adequate ventilation was maintained despite the use of a narrow-bore ETT. Rescue medications and airway management tools were also prepared in advance to promptly address any unexpected complications. Recently, Wang proposed a new classification method for the central airway, suggesting that the central airway should be divided into three equal areas: I, II, and III. The carina is classified as the IV zone, and the right central and middle bronchus are the V and VI zones. The left principal bronchus is divided into VII and VIII zone [ 15 ] . This classification method helps us select the distal location of the ETT according to the location of the lesion. The method proposed in this paper requires ensuring that the inflatable balloon is firmly occluded distally while maintaining oxygenation, which is primarily applicable to lesions in the I, II, and the upper half of the III zones, especially those in zone I (subglottic airway). Although we have not tried it, we believe this approach also applies to lesions located in zone IV or lower zone III. At this point, we can insert the distal end of the ETT into the right main bronchus or the corresponding trachea with more effective lung tissue as determined by chest CT and allow balloon inflation to embed the distal end. Of course, this procedure may sometimes require splicing of the ETTs (customized ETT assembled by connecting 5.5 mm and 6.0 mm ID tubes) ( Fig. 6 ) . However, we have not yet applied this technique to distal lesions in clinical practice, and we acknowledge that the spliced ETT design may carry a risk of dislodgement. Further studies are required to assess the safety, feasibility, and stability of this approach in such cases. We believe that biopsy or interventional treatment of subglottic lesions or even all central airways lesions with a flexible bronchoscope under the protection of ETT is safe, effective, and feasible. The proposal of this method will help solve the dilemma caused by the inability to ensure the safe implementation of rigid bronchoscopy or the inability to perform interventional vascular therapy and may also provide more options for the urgent treatment of the subglottic lesion. However, its efficacy may be limited in cases involving bulky tumors causing near-complete obstruction, or when balloon occlusion is technically unfeasible. Before performing this approach, we must communicate in detail with the anesthesiologist to assess the feasibility of this surgical approach applied to each patient. Furthermore, when some centers are not equipped to handle complex central airway lesions, patients should be rapidly transferred to an appropriate medical facility. Further multicenter studies are still needed to follow up to determine whether this approach can be safely and effectively applied to different institutions and patient populations. Declarations Funding: This study was supported by The National Natural Science Foundation of China (81970015) and Medical Health Science and Technology Project of Zhejiang Provincial Health Commission (2024KY1868) Author Contribution (I)Conception and design: H li; (II) Administrative support: H li; (III) Provision of study materials or patients: H li, H Lin, T Ji; (IV) Collection and assembly of data: T Ji, H Lin;(V) Data analysis and interpretation: T Ji, H Lin, H Zheng, W Ma, S Song, Y Zheng, D Yu, L Ye; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors. Availability of Data and Materials: The datasets used and/or analysed during the current study available from the corresponding author on reasonable request. Conflicts of Interest : All authors have completed the ICMJE uniform disclosure form. The authors have no conflicts of interest to declare. Ethical Statement: Written informed consent was obtained from the patients for the publication of any potentially identifiable data or clinical images. As this report involved a retrospective description of clinical cases with no additional risk to the patients, ethical approval was waived by the Institutional Review Board of the First Affiliated Hospital, Zhejiang University School of Medicine, in accordance with institutional policy. References Dunlap DG, Ravenel J, Sechrist J, Semaan R. Interventional Therapies for Central Airways. Journal of thoracic imaging 2019; 34: W49-w59. Batra H, Yarmus L. Indications and complications of rigid bronchoscopy. Expert review of respiratory medicine 2018; 12: 509-520. Ren. J, Huang. Hd, Wang. Q, Yang. Yg, Huang. Y, Lu. Q, Bai. C. Rigid bronchoscopy in the real world: controversies and thinking. Academic Journal of Second Military Medical University 2018; 39: 7. Diaz-Mendoza J, Peralta AR, Debiane L, Simoff MJ. Rigid Bronchoscopy. Seminars in respiratory and critical care medicine 2018; 39: 674-684. Somri M, Barna Teszler C, Tome R, Kugelman A, Vaida S, Gaitini L. Flexible fiberoptic bronchoscopy through the laryngeal mask airway in a small, premature neonate. American journal of otolaryngology 2005; 26: 268-271. Wu. Xiaoyu, Huachun C. Clinical retrospective analysis of interventional treatment of subglottic tracheal malignant tumors by bronchoscopy. China higher medical education 2018: 2. Alon D, Pertzov B, Gershman E, Frishman M, Rahman NA, Rosengarten D, Kramer MR. The Safety of Laryngeal Mask Airway-Assisted Bronchoscopy versus Standard Nasal Bronchoscopy. Respiration; international review of thoracic diseases 2017; 93: 279-284. Shao H, Wu J, Wu Q, Sun X, Li L, Xing Z, Sun H. Bronchial artery embolization for hemoptysis: a retrospective observational study of 344 patients. Chin Med J (Engl). 2015;128(1):58-62. Nicelli E, Gemma M, De Vitis A, Foti G, Beretta L. Feasibility of standard mechanical ventilation with low FiO2 and small endotracheal tubes during laser microlaryngeal surgery. Head Neck. 2010;32(2):204-209. Murgu SD, Colt HG. Complications of silicone stent insertion in patients with expiratory central airway collapse. The Annals of thoracic surgery 2007; 84: 1870-1877. Di Felice C, Alraiyes AH, Gillespie C, Machuzak M, Gildea TR, Sethi S, et al. Short-term Endoscopic Outcomes of Balloon and Rigid Bronchoplasty in the Management of Benign Subglottic and Tracheal Stenosis. J Bronchology Interv Pulmonol. 2023;30(1):54-59. Chaddha U, Murgu S. Complications of rigid bronchoscopy. Respirology. 2021 Jan;26(1):14-18. Hu HC, Lin SY, Hung YT, Chang SY. Feasibility and Associated Limitations of Office-Based Laryngeal Surgery Using Carbon Dioxide Lasers. JAMA Otolaryngol Head Neck Surg. 2017;143(5):485-491. Lim WY, Wong P. Awake supraglottic airway guided flexible bronchoscopic intubation in patients with anticipated difficult airways: a case series and narrative review. Korean J Anesthesiol. 2019 Dec;72(6):548-557. Wang. H. New classification system of eight regions in central airway and therapy strategy of malignant airway noeplasma. Clinical Focus 2016; 31: 1167-1169. Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 28 Apr, 2025 Read the published version in European Journal of Medical Research → Version 1 posted Editorial decision: Accepted 17 Apr, 2025 Reviews received at journal 15 Apr, 2025 Reviewers agreed at journal 15 Apr, 2025 Reviewers agreed at journal 14 Apr, 2025 Reviewers invited by journal 13 Apr, 2025 Submission checks completed at journal 11 Apr, 2025 First submitted to journal 10 Apr, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5050685","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":442553155,"identity":"e87c97b0-6b32-4f28-9451-99406f787b90","order_by":0,"name":"huihui Lin","email":"","orcid":"","institution":"First Affiliated Hospital of Zhejiang University School of Medicine","correspondingAuthor":false,"prefix":"","firstName":"huihui","middleName":"","lastName":"Lin","suffix":""},{"id":442553156,"identity":"ec2c3ad3-4c2f-4ab7-baf1-f24513d3fc95","order_by":1,"name":"Tingfen Ji","email":"","orcid":"","institution":"First Affiliated Hospital of 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01:38:22","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-5050685/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-5050685/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1186/s40001-025-02615-2","type":"published","date":"2025-04-28T15:57:08+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":80729275,"identity":"eaa63154-ce31-49d0-85f9-b2c4b4721d09","added_by":"auto","created_at":"2025-04-16 12:18:55","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":29893,"visible":true,"origin":"","legend":"\u003cp\u003eThe schematic diagrams of this method.\u003c/p\u003e","description":"","filename":"1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5050685/v1/7e4a34a04d73a591c81085c7.jpg"},{"id":80730367,"identity":"79cebf74-37a8-4605-8805-536119ff7dc2","added_by":"auto","created_at":"2025-04-16 12:26:55","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":101201,"visible":true,"origin":"","legend":"\u003cp\u003eThe subglottic lesions of two patients.\u003c/p\u003e","description":"","filename":"2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5050685/v1/0abe12da6e12c341a8a73055.jpg"},{"id":80729279,"identity":"a8a648d3-2c6d-4c61-b91b-8d789e27e0c5","added_by":"auto","created_at":"2025-04-16 12:18:55","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":85963,"visible":true,"origin":"","legend":"\u003cp\u003eThis inflated balloon has firmly blocked the distal end of the trachea.\u003c/p\u003e","description":"","filename":"3.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5050685/v1/750a5ba09f296b9714a8ab33.jpg"},{"id":80729280,"identity":"cd871fa9-c027-4604-bb1d-16e867ac829b","added_by":"auto","created_at":"2025-04-16 12:18:55","extension":"jpg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":59343,"visible":true,"origin":"","legend":"\u003cp\u003eAt first, we placed the tracheal catheter to the distal end of the lesion and inflated the distal end; under the protection of tracheal intubation, a fiberoptic bronchoscope was used to extract and biopsy the lump; after the treatment, we withdrew the fiberoptic bronchoscope and tracheal catheter from the airway.\u003c/p\u003e","description":"","filename":"4.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5050685/v1/a581f5871641c829649362cf.jpg"},{"id":80730368,"identity":"67905897-8af2-4176-bb06-9b9bf24c4a71","added_by":"auto","created_at":"2025-04-16 12:26:55","extension":"jpg","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":109223,"visible":true,"origin":"","legend":"\u003cp\u003e(A) Before treatment, the patient had severe subglottic stenosis; (B)This treatment augments the airway and restores airway patency.\u003c/p\u003e","description":"","filename":"5.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5050685/v1/26fbbee453cdb6333ef7a40f.jpg"},{"id":80730840,"identity":"b915e56e-28f8-4610-a1ef-b8c9f7d67f6b","added_by":"auto","created_at":"2025-04-16 12:34:55","extension":"jpg","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":31676,"visible":true,"origin":"","legend":"\u003cp\u003eThis is a long tracheal tube formed by splicing two tracheal tubes.\u003c/p\u003e","description":"","filename":"6.jpg","url":"https://assets-eu.researchsquare.com/files/rs-5050685/v1/fa2cfc90317a09ad94e9f29c.jpg"},{"id":81987472,"identity":"72f167be-a72d-47cc-b6ef-ee93dbe2ed7b","added_by":"auto","created_at":"2025-05-05 16:03:49","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":935366,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-5050685/v1/00d29eaf-5036-4219-b09e-45777b5f1541.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Biopsy and Interventional Therapy of Subglottic Lesions with Flexible Bronchoscope under Protection of Endotracheal Intubation","fulltext":[{"header":"Introduction","content":"\u003cp\u003eVarious benign and malignant airway diseases, especially central airway diseases, can result in various clinical symptoms, including coughing, wheezing, dyspnea, and recurrent airway infections distal to the lesion\u003csup\u003e[\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e]\u003c/sup\u003e. In addition, central airway diseases are usually rich in blood supply and can cause fatal bleeding when the lesion ruptures. Without timely intervention, asphyxia and even death can occur at any time\u003csup\u003e[\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e]\u003c/sup\u003e. Therefore, central airway diseases require early intervention.\u003c/p\u003e \u003cp\u003eThe rigid bronchoscope has a large orifice for easy operation while maintaining airway patency and respiratory support\u003csup\u003e[\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e]\u003c/sup\u003e. If there is heavy bleeding during the operation, a large-aperture suction tube can be used to ensure a clear field of view while argon plasma coagulation (APC), high-frequency electric knife (HFEK), and laser photocoagulation are utilized to stop the bleeding. Additionally, the sharp angle at the tip of the hard metal bronchoscope tube allows for direct \"removal\" of the tumor under the bronchoscope\u003csup\u003e[\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e]\u003c/sup\u003e. Therefore, rigid bronchoscopy is often the first choice for treating central airway diseases\u003csup\u003e[\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e]\u003c/sup\u003e.\u003c/p\u003e \u003cp\u003eHowever, the subglottic lesion poses a challenge to rigid bronchoscopy due to the restricted field of view. When dealing with subglottic lesions, a laryngeal mask airway (LMA) is usually used for airway management. The LMA facilitates the insertion of the flexible bronchoscope at the level of the glottic opening and provides excellent visualization of the anatomy and dynamics of the glottis, subglottic area, and other parts of the airway. It also maintains airway patency during general anesthesia, permitting spontaneous respiration and allowing for assisted ventilation if necessary\u003csup\u003e[\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e]\u003c/sup\u003e. Therefore, an LMA combined with a flexible bronchoscope is the most common choice for these lesions\u003csup\u003e[\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e]\u003c/sup\u003e.\u003c/p\u003e \u003cp\u003eBut the use of LMA has some shortcomings. When the blood supply of the lesion is abundant and intraoperative bleeding occurs, it is hard to control the bleeding and ensure adequate ventilation with an LMA\u003csup\u003e[\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e]\u003c/sup\u003e. Although the rapidly developing vascular interventional techniques in recent years can effectively reduce the risk of bleeding by embolizing the lesion vessels to achieve preoperative prevention or intraoperative hemostasis, there will always be various circumstances that limit its application\u003csup\u003e[\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e]\u003c/sup\u003e. For example, the onset of subglottic lesion is often ferocious. Patients tend to visit grass-roots hospitals, and it is difficult for them to tolerate long-distance transport. In addition, the medical unit lacks the means to perform bronchial artery embolization, the condition is too urgent to allow for vascular intervention, or the patient has an iodine allergy.\u003c/p\u003e \u003cp\u003eThis paper will report a safe and effective method for biopsy or interventional therapy via a flexible bronchoscope under the protection of endotracheal intubation. A flexible bronchoscope was placed in the gap between the endotracheal tube (ETT) and the trachea. Biopsy forceps, APC, CO\u003csub\u003e2\u003c/sub\u003e cryotherapy, and other equipment were used to perform operations such as biopsy and tumor removal through the working orifice of the flexible bronchoscope. Balloon incarceration in the distal airway of the lesion can effectively prevent the mass from falling off and the risk of suffocation caused by severe bleeding \u003cb\u003e(\u003c/b\u003eFig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e\u003cb\u003e)\u003c/b\u003e.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e"},{"header":"Methods and Results","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e \u003ch2\u003ePatient 1\u003c/h2\u003e \u003cp\u003eA 45-year-old female with an 8-day history of hemoptysis was admitted to the respiratory department on October 12, 2019. She had a history of thyroidectomy six years prior and was known to be allergic to iodinated contrast media. Chest computed tomography (CT) revealed a mural nodule in the upper trachea, approximately 1 cm below the glottis. Bronchoscopy identified a subglottic nodular bulge arising from the airway mucosa, which appeared hypervascular (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eA).\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003c/div\u003e\n\u003ch3\u003ePatient 2\u003c/h3\u003e\n\u003cp\u003eA 62-year-old male was admitted to the respiratory department on August 02, 2021, with a 40-days history of intermittent dyspnea. His medical history included radical surgery and chemoradiotherapy for small cell lung cancer three years earlier, as well as rigid endotracheal tumor resection performed one year prior to admission. One month before admission, the patient experienced a sudden episode of coma lasting about one hour and underwent emergency tracheal intubation. Following the procedure, he developed recurrent hemoptysis. Bronchoscopy performed in July 2021 revealed a subglottic mucosal bulge (Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003eB). Based on chest CT conducted two weeks prior to symptom onset, upper airway tumor invasion was suspected. It was presumed that the majority of the tumor tissue had been dislodged during the intubation process.\u003c/p\u003e\n\u003ch3\u003ePreoperative Consideration\u003c/h3\u003e\n\u003cp\u003eIn both cases, a thorough preoperative evaluation was conducted to determine the most appropriate interventional strategy. Several commonly used approaches for the management of subglottic lesions were carefully assessed. The risks and feasibility of each method were thoroughly explained to the patients, and informed consent was obtained prior to the procedure.\u003c/p\u003e \u003cp\u003ePatient 1 had a known allergy to iodinated contrast media, which made vascular embolization infeasible. She also declined awake intubation due to anxiety and poor tolerance. Rigid bronchoscopy was considered but abandoned because the lesion was located only 1 cm below the glottis, leaving insufficient operating space for the rigid scope tip. LMA, though commonly used in such procedures, was also deemed unsuitable due to the rich vascularity of the lesion and the high risk of intraoperative bleeding that would be difficult to manage via LMA ventilation.\u003c/p\u003e \u003cp\u003ePatient 2 had a complex medical and surgical history, including radical resection and radiotherapy for small cell lung cancer three years earlier and rigid bronchoscopic tumor resection one year earlier. Due to the proximity of the lesion to the acoustic hilum, rigid bronchoscopy was deemed unsuitable due to limited surgical space. In addition, the anesthesiologist was concerned that positive pressure ventilation through the anesthesia machine might be compromised, which could lead to inadequate oxygenation. Intraoperative bleeding is also difficult to control with LMA-assisted ventilation.\u003c/p\u003e \u003cp\u003eBoth patients had subglottic lesions. Finally, we chose to apply flexible bronchoscopy under the protection of tracheal intubation for biopsy sampling in patient 1 and biopsy sampling and partial removal of tumor tissue in patient 2.\u003c/p\u003e\n\u003ch3\u003eAnesthesia\u003c/h3\u003e\n\u003cp\u003eTo reduce the stress response during intubation, lidocaine cream was applied to lubricate the ETT. General anesthesia was induced with intravenous administration of etomidate, alfentanil, and atracurium. Anesthesia was maintained using a combination of propofol, remifentanil, and sevoflurane. Epinephrine was prepared in advance as a rescue medication in case of emergency. ETT 4.5 (Covidien, Origin: Shanghai, Model: 18860, OD 6.6mm, ID 4.5mm) was selected, and tracheal intubation was performed under the guidance of a flexible bronchoscope. Under direct vision, the ETT was seen to cross the distal end of the lesion by approximately 2\u0026ndash;3 cm. The balloon was then inflated until the trachea was incarcerated (Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003e). The proximal end of the ETT is connected to a ventilator to maintain breathing;\u003c/p\u003e \u003cp\u003e \u003c/p\u003e\n\u003ch3\u003eBiopsy and Interventional Therapy\u003c/h3\u003e\n\u003cp\u003eThe flexible bronchoscope (Olympus, OD 5.5mm, ID 2.8mm) was placed in the gap between the ETT and the trachea. Various treatments can be performed through the working orifice of the flexible bronchoscope. Balloon incarceration in the distal airway of the lesion can effectively prevent the mass from falling off and the risk of suffocation caused by severe bleeding \u003cb\u003e(\u003c/b\u003eFig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003eA-D\u003cb\u003e)\u003c/b\u003e.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eIn patient 1, the bleeding volume after the biopsy was about 90 ml, which was stopped by 1:1000 epinephrine plus 4\u0026deg;C ice physiological saline and APC. During the operation, the patient had stable oxygenation and blood pressure and no significant bleeding from the distal airway after removing the tracheal intubation balloon. The operation time was near 32 minutes. In patient 2, In the second case, the bleeding volume was about 10 ml after the biopsy, followed by CO\u003csub\u003e2\u003c/sub\u003e cryotherapy and APC to eliminate part of the tumor tissue. The operation time was 45 minutes.\u003c/p\u003e"},{"header":"Discussion","content":"\u003cp\u003eIn clinical practice, biopsy or intervention for the subglottic lesion can be a high-risk and challenging task. As far as we know, we are the first to report this method, which is relatively safe, practical, feasible, and easy to apply. Undoubtedly, the most significant disadvantage of this method is that the ETT occupies airway space, which limits bronchoscopy, while the limited operating space might make it difficult to use thermal therapy such as laser therapy or APC near flammable tubes. Therefore, we recommend choosing an ETT with an ID of 4.5 mm to reserve space for subsequent surgery, which can avoid the exposure of thermal therapy to the flammable ETT as much as possible. Additionally, microlaryngoscopy tubes (MLTs), which are specifically designed for airway procedures in narrow spaces, may serve as a safer and more standardized alternative to conventional ETTs. Their potential advantages in minimizing space occupation and improving intraoperative safety warrant further exploration in future practice\u003csup\u003e[\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e]\u003c/sup\u003e.\u003c/p\u003e \u003cp\u003eIn fact, including the above two cases, we have also used this approach to remove granulation by laser therapy in a patient who developed a stricture above the incision with a post-tracheostomy (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eA). Previous experience showed that the patient had severe subglottic capillary leakage and that the lesion was prone to bleeding when touched. It is worth noting that, clinically, when faced with this situation, silicone stents were mostly chosen to be placed in the airway. However, severe granulation tissue growth can still occur after stent placement\u003csup\u003e[\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e]\u003c/sup\u003e. Therefore, we first performed balloon dilation (diameter 15mm) at the airway stenosis and then treated the lesion with laser therapy using the method described above. Remarkably, the procedure went very well, and the airway recovered well at the 6-month follow-up that has continued since the surgery \u003cb\u003e(\u003c/b\u003eFig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003eB\u003cb\u003e)\u003c/b\u003e. Subglottic stenosis remains a particularly challenging condition due to the narrow anatomical structure and risk of airway compromise. Di Felice et al.'s retrospective comparison of rigid bronchoscopy versus flexible bronchoscopy under general anesthesia found no significant differences in outcomes\u003csup\u003e[\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e]\u003c/sup\u003e. Although rigid bronchoscopy offers advantages in instrument capacity, the widespread availability and relative simplicity of balloon catheters delivered through flexible bronchoscopes make this approach more accessible, particularly for operators without specialized training in rigid techniques\u003csup\u003e[\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e]\u003c/sup\u003e.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eWhen encountering subglottic masses, several approaches are commonly considered, including rigid bronchoscopy, awake fiberoptic bronchoscopy, bronchial artery embolization, and flexible bronchoscopy with LMA. Among these, awake fiberoptic bronchoscopy is considered one of the safest options as it preserves spontaneous breathing and patient awareness. However, in therapeutic procedures that require prolonged operation time, patients may experience significant discomfort, and lack of cooperation can increase the risk of airway injury. Additionally, Chang et al. conducted a study on 49 patients with laryngeal lesions who underwent carbon dioxide laser surgery. They found that while there was significant improvement in voice function for patients with benign lesions and Reinke's edema, limitations in tolerance and therapeutic efficacy were observed\u003csup\u003e[\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e]\u003c/sup\u003e. This may be analogous to awake fiberoptic bronchoscopy, which could be a safer choice for lesions with shorter expected procedural times, provided the patient has a high tolerance. Notably, for patients with anticipated difficult airways, awake fiberoptic bronchoscopy via the oral route may also be a viable option\u003csup\u003e[\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e]\u003c/sup\u003e. Rigid bronchoscopy offers a wide operating channel and relatively safe airway control, but it is often unsuitable for subglottic lesions due to limited accessibility and may cause mechanical trauma to the airway. LMA-assisted bronchoscopy maintains ventilation and offers procedural flexibility but is not ideal for highly vascular lesions, where bleeding is difficult to control through LMA ventilation alone. For hypervascular tumors, bronchial artery embolization may be effective in reducing intraoperative bleeding. However, this technique does not provide histological diagnosis or immediate lesion removal. In addition, embolization is contraindicated in patients with contrast agent allergies, as was the case in Patient 1. Comparison of Different Management Techniques for Subglottic Lesions shown in Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e.\u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab1\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eComparison of Different Management Techniques for Subglottic Lesions\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"3\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eTechnique\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eAdvantages\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eDisadvantages\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFlexible bronchoscope under ETT Protection\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e- Good visualization of the subglottic area\u003c/p\u003e \u003cp\u003e- Balloon tamponade enables bleeding control\u003c/p\u003e \u003cp\u003e- Minimizes risk of tumor dislodgement\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e- Limited operative space\u003c/p\u003e \u003cp\u003e- Requires experienced bronchoscopist and anesthesiologist\u003c/p\u003e \u003cp\u003e- Requires tolerance to general anesthesia\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFlexible bronchoscope with LMA\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e- Preserves spontaneous respiration\u003c/p\u003e \u003cp\u003e- Suitable for short diagnostic procedures and therapeutic interventions\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e- Ineffective bleeding control\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eRigid bronchoscope\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e- Wide working channel\u003c/p\u003e \u003cp\u003e- Suitable for resection of large or hemorrhagic lesions\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e- Limited visualization of subglottic area\u003c/p\u003e \u003cp\u003e- Higher risk of airway trauma\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eInterventional embolization\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e- Minimally invasive\u003c/p\u003e \u003cp\u003e- Effective for hypervascular lesions\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e- Contraindicated in patients with iodine allergy\u003c/p\u003e \u003cp\u003e- Not allow biopsy or lesion resection\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eAwake fiberoptic intubation\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e- Contraindicated in patients with iodine allergy\u003c/p\u003e \u003cp\u003e- Does not allow biopsy or lesion resection\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e- Poor patient comfort\u003c/p\u003e \u003cp\u003e- Requires patient cooperation\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003ePotential complications of our method include airway trauma due to repeated scope manipulation, thermal injury from the use of APC or laser therapy near the ETT, and anesthesia-related events such as hypotension or delayed recovery. To minimize these risks, we emphasize the importance of careful patient selection and continuous intraoperative monitoring of hemodynamic parameters. The procedure should be performed by a team experienced in both bronchoscopy and airway management. In addition, airway management plays a critical role in this patient population. In both cases presented, oxygen saturation remained stable above 90%, end-tidal CO₂ (EtCO₂) levels were within the normal range (35\u0026ndash;45 mmHg), and peak airway pressure did not exceed 30cmH₂O. These parameters indicate that adequate ventilation was maintained despite the use of a narrow-bore ETT. Rescue medications and airway management tools were also prepared in advance to promptly address any unexpected complications.\u003c/p\u003e \u003cp\u003eRecently, Wang proposed a new classification method for the central airway, suggesting that the central airway should be divided into three equal areas: I, II, and III. The carina is classified as the IV zone, and the right central and middle bronchus are the V and VI zones. The left principal bronchus is divided into VII and VIII zone\u003csup\u003e[\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e]\u003c/sup\u003e. This classification method helps us select the distal location of the ETT according to the location of the lesion. The method proposed in this paper requires ensuring that the inflatable balloon is firmly occluded distally while maintaining oxygenation, which is primarily applicable to lesions in the I, II, and the upper half of the III zones, especially those in zone I (subglottic airway). Although we have not tried it, we believe this approach also applies to lesions located in zone IV or lower zone III. At this point, we can insert the distal end of the ETT into the right main bronchus or the corresponding trachea with more effective lung tissue as determined by chest CT and allow balloon inflation to embed the distal end. Of course, this procedure may sometimes require splicing of the ETTs (customized ETT assembled by connecting 5.5 mm and 6.0 mm ID tubes) \u003cb\u003e(\u003c/b\u003eFig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003e\u003cb\u003e)\u003c/b\u003e. However, we have not yet applied this technique to distal lesions in clinical practice, and we acknowledge that the spliced ETT design may carry a risk of dislodgement. Further studies are required to assess the safety, feasibility, and stability of this approach in such cases.\u003c/p\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003eWe believe that biopsy or interventional treatment of subglottic lesions or even all central airways lesions with a flexible bronchoscope under the protection of ETT is safe, effective, and feasible. The proposal of this method will help solve the dilemma caused by the inability to ensure the safe implementation of rigid bronchoscopy or the inability to perform interventional vascular therapy and may also provide more options for the urgent treatment of the subglottic lesion. However, its efficacy may be limited in cases involving bulky tumors causing near-complete obstruction, or when balloon occlusion is technically unfeasible. Before performing this approach, we must communicate in detail with the anesthesiologist to assess the feasibility of this surgical approach applied to each patient. Furthermore, when some centers are not equipped to handle complex central airway lesions, patients should be rapidly transferred to an appropriate medical facility. Further multicenter studies are still needed to follow up to determine whether this approach can be safely and effectively applied to different institutions and patient populations.\u003c/p\u003e"},{"header":"Declarations","content":"\u003ch2\u003eFunding:\u003c/h2\u003e \u003cp\u003eThis study was supported by The National Natural Science Foundation of China (81970015) and Medical Health Science and Technology Project of Zhejiang Provincial Health Commission (2024KY1868)\u003c/p\u003e\u003ch2\u003eAuthor Contribution\u003c/h2\u003e\u003cp\u003e(I)Conception and design: H li; (II) Administrative support: H li; (III) Provision of study materials or patients: H li, H Lin, T Ji; (IV) Collection and assembly of data: T Ji, H Lin;(V) Data analysis and interpretation: T Ji, H Lin, H Zheng, W Ma, S Song, Y Zheng, D Yu, L Ye; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors.\u003c/p\u003e\u003ch2\u003eAvailability of Data and Materials:\u003c/h2\u003e \u003cp\u003eThe datasets used and/or analysed during the current study available from the corresponding author on reasonable request.\u003c/p\u003e \u003cp\u003e \u003cb\u003eConflicts of Interest\u003c/b\u003e: All authors have completed the ICMJE uniform disclosure form. The authors have no conflicts of interest to declare.\u003c/p\u003e \u003cp\u003e Ethical Statement: Written informed consent was obtained from the patients for the publication of any potentially identifiable data or clinical images. As this report involved a retrospective description of clinical cases with no additional risk to the patients, ethical approval was waived by the Institutional Review Board of the First Affiliated Hospital, Zhejiang University School of Medicine, in accordance with institutional policy.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eDunlap DG, Ravenel J, Sechrist J, Semaan R. Interventional Therapies for Central Airways. \u003cem\u003eJournal of thoracic imaging \u003c/em\u003e2019; 34: W49-w59.\u003c/li\u003e\n\u003cli\u003eBatra H, Yarmus L. Indications and complications of rigid bronchoscopy. \u003cem\u003eExpert review of respiratory medicine \u003c/em\u003e2018; 12: 509-520.\u003c/li\u003e\n\u003cli\u003eRen. J, Huang. Hd, Wang. Q, Yang. Yg, Huang. Y, Lu. Q, Bai. C. Rigid bronchoscopy in the real world: controversies and thinking. \u003cem\u003eAcademic Journal of Second Military Medical University \u003c/em\u003e2018; 39: 7.\u003c/li\u003e\n\u003cli\u003eDiaz-Mendoza J, Peralta AR, Debiane L, Simoff MJ. Rigid Bronchoscopy. \u003cem\u003eSeminars in respiratory and critical care medicine \u003c/em\u003e2018; 39: 674-684.\u003c/li\u003e\n\u003cli\u003eSomri M, Barna Teszler C, Tome R, Kugelman A, Vaida S, Gaitini L. Flexible fiberoptic bronchoscopy through the laryngeal mask airway in a small, premature neonate. \u003cem\u003eAmerican journal of otolaryngology \u003c/em\u003e2005; 26: 268-271.\u003c/li\u003e\n\u003cli\u003eWu. Xiaoyu, Huachun C. 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Feasibility and Associated Limitations of Office-Based Laryngeal Surgery Using Carbon Dioxide Lasers.\u003cem\u003e JAMA Otolaryngol Head Neck Surg. \u003c/em\u003e2017;143(5):485-491.\u003c/li\u003e\n\u003cli\u003eLim WY, Wong P. Awake supraglottic airway guided flexible bronchoscopic intubation in patients with anticipated difficult airways: a case series and narrative review. \u003cem\u003eKorean J Anesthesiol.\u003c/em\u003e 2019 Dec;72(6):548-557. \u003c/li\u003e\n\u003cli\u003eWang. H. New classification system of eight regions in central airway and therapy strategy of malignant airway noeplasma. \u003cem\u003eClinical Focus \u003c/em\u003e2016; 31: 1167-1169.\u003c/li\u003e\n\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"european-journal-of-medical-research","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"ejmr","sideBox":"Learn more about [European Journal of Medical Research](http://eurjmedres.biomedcentral.com)","snPcode":"40001","submissionUrl":"https://submission.nature.com/new-submission/40001/3","title":"European Journal of Medical Research","twitterHandle":"@BioMedCentral","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Bronchoscopy, Subglottic Lesions, Endotracheal Intubation, Laryngeal mask airway","lastPublishedDoi":"10.21203/rs.3.rs-5050685/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-5050685/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eSubglottic lesions represent a complex and challenging clinical entity, often associated with high procedural risks due to their anatomical location and vascularity. While a laryngeal mask airway(LMA) is commonly employed for airway management in such cases, it has notable limitations, particularly in scenarios involving significant bleeding or the need for extended intervention. This article presents an alternative and effective approach for the biopsy and interventional treatment of subglottic lesions using a flexible bronchoscope under the protection of an endotracheal tube (ETT). By inserting the bronchoscope through the space between the ETT and the tracheal wall, various procedures\u0026mdash;including biopsy, argon plasma coagulation (APC), and CO₂ cryotherapy\u0026mdash;can be performed with relative safety. This method provides a feasible option for airway protection, bleeding control, and lesion management, offering new possibilities in clinical practice.\u003c/p\u003e","manuscriptTitle":"Biopsy and Interventional Therapy of Subglottic Lesions with Flexible Bronchoscope under Protection of Endotracheal Intubation","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-04-16 12:18:50","doi":"10.21203/rs.3.rs-5050685/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Accepted","date":"2025-04-17T19:09:49+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-04-15T21:43:01+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"253548222261916217862095482549869100431","date":"2025-04-15T10:45:08+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"212046318518626941567037900980319382717","date":"2025-04-14T07:44:59+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-04-13T10:35:48+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-04-11T06:06:00+00:00","index":"","fulltext":""},{"type":"submitted","content":"European Journal of Medical Research","date":"2025-04-11T03:07:21+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"european-journal-of-medical-research","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"ejmr","sideBox":"Learn more about [European Journal of Medical Research](http://eurjmedres.biomedcentral.com)","snPcode":"40001","submissionUrl":"https://submission.nature.com/new-submission/40001/3","title":"European Journal of Medical Research","twitterHandle":"@BioMedCentral","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"BMC/SO AJ","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"9035e02e-b6e1-4828-ab5c-6274020730a2","owner":[],"postedDate":"April 16th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2025-05-05T15:58:59+00:00","versionOfRecord":{"articleIdentity":"rs-5050685","link":"https://doi.org/10.1186/s40001-025-02615-2","journal":{"identity":"european-journal-of-medical-research","isVorOnly":false,"title":"European Journal of Medical Research"},"publishedOn":"2025-04-28 15:57:08","publishedOnDateReadable":"April 28th, 2025"},"versionCreatedAt":"2025-04-16 12:18:50","video":"","vorDoi":"10.1186/s40001-025-02615-2","vorDoiUrl":"https://doi.org/10.1186/s40001-025-02615-2","workflowStages":[]},"version":"v1","identity":"rs-5050685","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-5050685","identity":"rs-5050685","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}