A New Analytical Method for Determination Bisphenol A in Canned meat

In recent years, attention has grown to bisphenol A (BPA), as it is an important compound in the manufacture of food packaging materials and many other plastic materials. Therefore, the focus has been recently on studying the characteristics and migration of cans to food, as well as the harmful effect on health. In this research, a reverse phase high liquid chromatography coupled with UV-detector (HPLC-UV) was developed for determining of BPA in canned meat. For quantitative determination of BPA, the following experimental conditions were established: mobile phase (acetonitrile: water, 55:45 v/v), a flow rate of 1mL/min, column temperature of 25 o C, injection volume 20µL and UV-detection at 227 nm. To validate the analytical method, it was based on the following parameters: Linearity, precision, recovery, and limit detection. A good linear correlation with R 2  = 0.9989 was observed over the range of 1 to 500 mg/Kg. The limit of quantitation (LOQ) was measured to be 0.982 mg/Kg, while the limit of detection (LOD) was measured to be 0.294 mg/Kg. The extraction recoveries for BPA were 90.006% in canned tuna, 98.299% in canned luncheon. The precision of the method, based on relative standard deviations (RSD %), ranged from 1.77–3.44%. The proposed method was found to be suitable and praised for the determination of BPA 1.56 to 1.96 mg/Kg, 0.34 to 0.90 mg/Kg in canned tuna, and canned luncheon meat, respectively.