Chemical Proteomics Probes: Classification, Applications, and Future Perspectives in Proteome-wide Studies

Chemical Proteomics Probes: Classification, Applications, and Future Perspectives in Proteome-wide Studies | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL PROTEOMICS This is a preprint and has not been peer reviewed. Data may be preliminary. 28 April 2025 V1 Latest version Share on Chemical Proteomics Probes: Classification, Applications, and Future Perspectives in Proteome-wide Studies Authors : Xiaoyue Tan , Dan Wang , Yingrui Yao , Kaixuan Liu , Yuzhou Chen , Songsen Fu , and Feng Ni 0000-0001-8482-6755 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174586713.32434581/v1 Published PROTEOMICS Version of record Peer review timeline 327 views 362 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Chemical proteomics probes serve as critical tools for investigating small molecule--protein interactions within complex biological systems. Traditionally, they are categorized into covalent probes and photoaffinity probes. These probes have found extensive applications in bioactive molecule target identification, targeted ligand screening, enzyme activity monitoring, protein post-translational modification studies, and cross-omics integration. They facilitate drug target discovery, targeted ligand screening, dynamic evaluation of enzyme activities in disease contexts, protein modification mapping, and bridging proteomics with other omics platforms. Despite their significant utility, challenges remain in probe design optimization, reduction of non-specific interactions, and expansion of targetable proteomic landscapes. Future research efforts are expected to focus on the development of novel probes, the integration of chemical proteomics with structural biology and artificial intelligence, and the advancement of clinical applications. These innovations will deepen our understanding of protein functions and support the advancement of precision medicine. In this review, we summarize the classification and fundamental principles of chemical proteomics probes and provide an in-depth discussion of their diverse applications. Supplementary Material File (chemical proteomics probes classification, applications, and future perspectives in proteome-wide studies.pdf) Download 2.63 MB Information & Authors Information Version history V1 Version 1 28 April 2025 Peer review timeline Published PROTEOMICS Version of Record 31 Jul 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection PROTEOMICS Keywords applications challenges chemical proteomics classification Authors Affiliations Xiaoyue Tan Tianjin University of Traditional Chinese Medicine View all articles by this author Dan Wang Ningbo University View all articles by this author Yingrui Yao View all articles by this author Kaixuan Liu View all articles by this author Yuzhou Chen Tianjin University of Traditional Chinese Medicine View all articles by this author Songsen Fu Ningbo University View all articles by this author Feng Ni 0000-0001-8482-6755 [email protected] Ningbo University View all articles by this author Metrics & Citations Metrics Article Usage 327 views 362 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Xiaoyue Tan, Dan Wang, Yingrui Yao, et al. Chemical Proteomics Probes: Classification, Applications, and Future Perspectives in Proteome-wide Studies. Authorea . 28 April 2025. 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