A diagnostic primer pair to distinguish between wMel and wAlbB Wolbachia infection - A simplified real-time PCR approach for use in field collected Aedes aegypti in dengue reduction trials

Detection of the Wolbachia endosymbiont in Aedes aegypti mosquitoes through real-time PCR assays is widely used during and after Wolbachia releases in dengue reduction trials involving the w Mel and w AlbB strains. However, primers applied in current successful Wolbachia releases cannot be used in a single assay to distinguish between these strains. Here, we developed a new diagnostic primer pair wMA which can detect w Mel or w AlbB infection in the same assay. We also tested current Wolbachia primers and show that there is variation in their performance when they are used to assess the relative density of Wolbachia . The new wMA primers provide an accurate and efficient estimate of the presence and density of both Wolbachia infections.