AaCYPdwf, a new growth regulatory cytochrome p450 gene from Artemissia annua

AaCYPdwf, a new growth regulatory cytochrome p450 gene from Artemissia annua | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article AaCYPdwf, a new growth regulatory cytochrome p450 gene from Artemissia annua Mahmuda Umme Rayhan, Tofazzal Islam, Soo-un Kim This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1560430/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Artemisia annua L. produces artemisinin (qinghaosu), which is inhibitory against malarial parasite, Plasmodium falciparum . Genetic engineering is thought to be one of the most reasonable approaches to enhance the production of artemisinin by A. annua plant. This study aimed to clone and investigate a growth regulatory cytochrome p450 gene from A. annua . We cloned a cDNA coding growth regulatory gene from A. annua . The gene had high homology with the steroid 23alpha-hydroxylase produced-gene of A. thaliana, Oryza sativa and Zinnia elegans . The full-length cDNA was 1708 bp long, containing an open reading frame of 1443 bp that encodes 480 amino acids. We transformed A. annua plants with the full-length cDNA driven by 35S promoter through Agrobacterium -mediated transformation. To identify the function of the gene, we applied RNAi gene silencing technique. The plants transformed with the full-length cDNA were normal in growth but the plants transformed with RNAi showed extreme dwarfism. The dwarfism in the gene silenced plants was rescued by the application of brassinolide and gibberellin hormones indicating that the mutants were deficient in the synthesis of a growth regulatory gene which we named AaCYPdwf . Phenotypic characteristics of mutant and phylogenetic analysis clustered the AaCYPdwf with other brassinolide producing genes. Our results suggest that AaCYPdwf is a new gene which likely involved in regulation of brassinosteroid biosynthesis in A. annua plants. Artemisia annua cytochrome P450 overexpression RNAi Full Text Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1560430","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":99778725,"identity":"bd6324e3-af12-42a6-91f5-53317e8f2af3","order_by":0,"name":"Mahmuda Umme Rayhan","email":"","orcid":"","institution":"Seoul National University Agriculture and Life Sciences Library: Seoul National University College of Agriculture and Life Sciences","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Mahmuda","middleName":"Umme","lastName":"Rayhan","suffix":""},{"id":99778726,"identity":"c28130d5-ac0b-4fc4-a706-d249954114af","order_by":1,"name":"Tofazzal Islam","email":"data:image/png;base64,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","orcid":"https://orcid.org/0000-0002-7613-0261","institution":"Bangabandhu Sheikh Mujibur Rahman Agricultural University","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Tofazzal","middleName":"","lastName":"Islam","suffix":""},{"id":99778727,"identity":"3e743eee-df68-4b9a-8b46-c96fb56171c9","order_by":2,"name":"Soo-un Kim","email":"","orcid":"","institution":"Seoul National University Agriculture and Life Sciences Library: Seoul National University College of Agriculture and Life Sciences","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Soo-un","middleName":"","lastName":"Kim","suffix":""}],"badges":[],"createdAt":"2022-04-15 06:33:37","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-1560430/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-1560430/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":20566622,"identity":"2fd097ee-c52b-4238-912a-b31155960e1c","added_by":"auto","created_at":"2022-04-20 17:00:16","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1688439,"visible":true,"origin":"","legend":"","description":"","filename":"ManuscriptSubmitted.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1560430/v1_covered.pdf"}],"financialInterests":"","formattedTitle":"AaCYPdwf, a new growth regulatory cytochrome p450 gene from Artemissia annua","fulltext":[{"header":"Full Text","content":"This preprint is available for \u003ca href='/article/rs-1560430/latest.pdf' target='_blank'\u003edownload as a PDF\u003c/a\u003e."}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Artemisia annua, cytochrome P450, overexpression, RNAi","lastPublishedDoi":"10.21203/rs.3.rs-1560430/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-1560430/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Artemisia annua L. produces artemisinin (qinghaosu), which is inhibitory against malarial parasite, Plasmodium falciparum . Genetic engineering is thought to be one of the most reasonable approaches to enhance the production of artemisinin by A. annua plant. This study aimed to clone and investigate a growth regulatory cytochrome p450 gene from A. annua . We cloned a cDNA coding growth regulatory gene from A. annua . The gene had high homology with the steroid 23alpha-hydroxylase produced-gene of A. thaliana, Oryza sativa and Zinnia elegans . The full-length cDNA was 1708 bp long, containing an open reading frame of 1443 bp that encodes 480 amino acids. We transformed A. annua plants with the full-length cDNA driven by 35S promoter through Agrobacterium -mediated transformation. To identify the function of the gene, we applied RNAi gene silencing technique. The plants transformed with the full-length cDNA were normal in growth but the plants transformed with RNAi showed extreme dwarfism. The dwarfism in the gene silenced plants was rescued by the application of brassinolide and gibberellin hormones indicating that the mutants were deficient in the synthesis of a growth regulatory gene which we named AaCYPdwf . Phenotypic characteristics of mutant and phylogenetic analysis clustered the AaCYPdwf with other brassinolide producing genes. Our results suggest that AaCYPdwf is a new gene which likely involved in regulation of brassinosteroid biosynthesis in A. annua plants.","manuscriptTitle":"AaCYPdwf, a new growth regulatory cytochrome p450 gene from Artemissia annua","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2022-04-20 17:00:07","doi":"10.21203/rs.3.rs-1560430/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"16973672-061b-43c8-a5a7-94e6c6dd8ee3","owner":[],"postedDate":"April 20th, 2022","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2022-04-20T17:00:09+00:00","versionOfRecord":[],"versionCreatedAt":"2022-04-20 17:00:07","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-1560430","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-1560430","identity":"rs-1560430","version":["v1"]},"buildId":"7rjqhiLT3MXkJMwkYKINL","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}