Fluctuating internal states mediate neural-behavioral covariations in V1

Fluctuating internal states mediate neural-behavioral covariations in V1 | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Biological Sciences - Article Fluctuating internal states mediate neural-behavioral covariations in V1 Eyal Seidemann, Baowang Li, Jason Samonds, Yuzhi Chen, Thibaud Taillefumier, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4741076/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted You are reading this latest preprint version Abstract Our perception of the world depends on a complex interplay between external sensory inputs and our internal states. How and where in the brain the interactions between internal states and sensory inputs are implemented remain open questions. To study the neural basis of these interactions, we used whole-cell recording to measure membrane potential (Vm) of single V1 neurons in macaque monkeys performing a reaction-time detection task. We find that most V1 neurons gradually depolarize in preparation for target onset. Remarkably, trial-to-trial variations in this preparatory buildup are correlated with the monkey’s reaction times, and these covariations are similar when the animal selects a target in either hemifield. This finding implies that variation in the preparatory buildup are shared between hemispheres and are unrelated to a competitive spatial attention mechanism. Further, we find that while the gradual buildup is largely uncorrelated with the animals’ choices, transient fluctuations in Vm around target-evoked response onset time are correlated with choice. The dependence of these Vm-to-choice covariations on the visual stimulus is consistent with a multiplicative gain, reflecting a variable internal state that interacts with variable sensory signals. Overall, our results reveal surprising covariations between the membrane potential of single V1 neurons and behavior, and that these covariations are mediated by mixture of additive and multiplicative task-related modulations of incoming sensory information. Biological sciences/Neuroscience/Neuronal physiology/Membrane potential Biological sciences/Neuroscience/Cognitive neuroscience/Attention Full Text Additional Declarations There is NO Competing Interest. Supplementary Files DetectionSupplementary.pdf Supplementary information Cite Share Download PDF Status: Under Review Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4741076","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Biological Sciences - Article","associatedPublications":[],"authors":[{"id":330937226,"identity":"4eb5960b-dae2-452f-a93d-4bf7bbe6aca8","order_by":0,"name":"Eyal Seidemann","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA+UlEQVRIiWNgGAWjYHACxgMfQBQzshgPAT0HZ4AUgbUkEKnlMA9cETFa+CWSHxy2qTlsb8/Oe4CZ94dNYn//AcYHb9twa5GckWZwOOfY4cQeZr4EZp6ENGOJGwnMhnPxaDG4kQDUwnY4gYeZxwCo5bAcww0GNmlePFrsb6R/OGzx77A9VMt/HvnzB9h/49NiIJFjcJix7TBjD0TLATmDAwlszPi0SJx5U3Cwty89secwj8HBOWnJxoY3Epsl55zDrYW/PX3jgx/frO3Z+88YPnhjY5c47/zhgx/elOHWwiCQgGAfgFCMDXjUg6w5gF9+FIyCUTAKRgEDAHBNTmLiBHB4AAAAAElFTkSuQmCC","orcid":"","institution":"The University of Texas at Austin","correspondingAuthor":true,"prefix":"","firstName":"Eyal","middleName":"","lastName":"Seidemann","suffix":""},{"id":330937227,"identity":"7af35b8d-9f0b-486b-82b4-dc2919930fe2","order_by":1,"name":"Baowang Li","email":"","orcid":"","institution":"The University of Texas at Austin","correspondingAuthor":false,"prefix":"","firstName":"Baowang","middleName":"","lastName":"Li","suffix":""},{"id":330937228,"identity":"4296db71-9d46-4bd5-b280-7adeb3a01d3f","order_by":2,"name":"Jason Samonds","email":"","orcid":"https://orcid.org/0000-0002-5857-0597","institution":"University of Texas at Austin","correspondingAuthor":false,"prefix":"","firstName":"Jason","middleName":"","lastName":"Samonds","suffix":""},{"id":330937229,"identity":"3c139f91-cd02-4b67-8e7d-55f48cd1c57e","order_by":3,"name":"Yuzhi Chen","email":"","orcid":"","institution":"The University of Texas at Austin","correspondingAuthor":false,"prefix":"","firstName":"Yuzhi","middleName":"","lastName":"Chen","suffix":""},{"id":330937230,"identity":"3df2af8f-f53c-4138-a1cd-7428a881c34e","order_by":4,"name":"Thibaud Taillefumier","email":"","orcid":"","institution":"UT Austin","correspondingAuthor":false,"prefix":"","firstName":"Thibaud","middleName":"","lastName":"Taillefumier","suffix":""},{"id":330937231,"identity":"e31a06ed-e39a-4b56-bf8c-9632069affd0","order_by":5,"name":"Nicholas Priebe","email":"","orcid":"https://orcid.org/0000-0002-0171-0691","institution":"The University of Texas at Austin","correspondingAuthor":false,"prefix":"","firstName":"Nicholas","middleName":"","lastName":"Priebe","suffix":""}],"badges":[],"createdAt":"2024-07-15 06:30:18","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4741076/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4741076/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":68663013,"identity":"2a491f6f-1251-4f92-b096-e54207d916c9","added_by":"auto","created_at":"2024-11-10 16:48:27","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1183821,"visible":true,"origin":"","legend":"Article File","description":"","filename":"Vmdetection.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4741076/v1_covered_27921f2f-9817-4b46-b5f5-9d0f8dae96fc.pdf"},{"id":61045561,"identity":"22721207-7b0b-40c2-a5c8-29216ce0c647","added_by":"auto","created_at":"2024-07-25 03:03:54","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":2058144,"visible":true,"origin":"","legend":"Supplementary information","description":"","filename":"DetectionSupplementary.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4741076/v1/4949966599ac920b32c26e6a.pdf"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"Fluctuating internal states mediate neural-behavioral covariations in V1","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"nature-portfolio","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"","title":"Nature Portfolio","twitterHandle":"","acdcEnabled":false,"dfaEnabled":false,"editorialSystem":"ejp","reportingPortfolio":"","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-4741076/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4741076/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Our perception of the world depends on a complex interplay between external sensory inputs and our internal states. How and where in the brain the interactions between internal states and sensory inputs are implemented remain open questions. To study the neural basis of these interactions, we used whole-cell recording to measure membrane potential (Vm) of single V1 neurons in macaque monkeys performing a reaction-time detection task. We find that most V1 neurons gradually depolarize in preparation for target onset. Remarkably, trial-to-trial variations in this preparatory buildup are correlated with the monkey’s reaction times, and these covariations are similar when the animal selects a target in either hemifield. This finding implies that variation in the preparatory buildup are shared between hemispheres and are unrelated to a competitive spatial attention mechanism. Further, we find that while the gradual buildup is largely uncorrelated with the animals’ choices, transient fluctuations in Vm around target-evoked response onset time are correlated with choice. The dependence of these Vm-to-choice covariations on the visual stimulus is consistent with a multiplicative gain, reflecting a variable internal state that interacts with variable sensory signals. Overall, our results reveal surprising covariations between the membrane potential of single V1 neurons and behavior, and that these covariations are mediated by mixture of additive and multiplicative task-related modulations of incoming sensory information.","manuscriptTitle":"Fluctuating internal states mediate neural-behavioral covariations in V1","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-07-25 03:03:49","doi":"10.21203/rs.3.rs-4741076/v1","editorialEvents":[],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"nature-neuroscience","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"neuro","sideBox":"Learn more about [Nature Neuroscience](http://www.nature.com/neuro/)","snPcode":"","submissionUrl":"","title":"Nature Neuroscience","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"ejp","reportingPortfolio":"Nature Research","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"55ef4427-7b47-4a6d-9cb5-b6747c5fac89","owner":[],"postedDate":"July 25th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[{"id":35036482,"name":"Biological sciences/Neuroscience/Neuronal physiology/Membrane potential"},{"id":35036483,"name":"Biological sciences/Neuroscience/Cognitive neuroscience/Attention"}],"tags":[],"updatedAt":"2025-07-18T15:01:58+00:00","versionOfRecord":[],"versionCreatedAt":"2024-07-25 03:03:49","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4741076","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4741076","identity":"rs-4741076","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}