Steps of DNA extraction for molecular analysis of microalgae communities - a simple guide for beginners

2024 · doi:10.3897/arphapreprints.e128742
preprint OA: closed
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Abstract

One of the biggest challenges in the microalgae industry is the lack of reliable methods to detect and avoid biological contaminants, including parasites, which can affect cell growth and consequently lead to crash of the culture. In recent years, the metabarcoding approach has been widely used to study diversity and distribution of microalgae communities and is also a promising tool for contaminant detection in routine biomonitoring. The aim of this article presents steps of DNA extraction for molecular analysis of microalgae communities.
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Preprint ARPHA Preprints https://doi.org/10.3897/arphapreprints.e128742 (03 Jun 2024) https://doi.org/10.3897/arphapreprints.e128742 (03 Jun 2024) Submitted to Research Ideas and Outcomes Other versions: - Preprint InfoPreprint Info - CiteCite - MetricsMetrics - CommentComment - RelatedRelated - CitedCited ARPHA Preprints doi: 10.3897/arphapreprints.e128742 First posted 03 Jun 2024 Authors Tena Radočaj - Corresponding author University of Zagreb, Faculty of Agriculture, Zagreb, Croatia University of Zagreb, Faculty of Agriculture, Zagreb, Croatia Université Savoie Mont Blanc, INRAE, CARRTEL, Thonon les Bains, France Laboratory for Aquaculture and Pathology of Aquatic Organisms Division for Marine and Environmental Research Rudjer Boskovic Institute, Zagreb, Croatia MJD Consulting, Croatia, Hvar, Croatia University of Zagreb , Zagreb, Croatia Conflict of interest The authors have declared that no competing interests exist. Supporting agencies ParAqua COST Action This is an open access preprint distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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