Differential Proteomic Analysis of Endometriosis

Differential proteins expressing in ectopic and eutopic endometria were investigated by means of proteo- mic analysis. Five patients in secretary phase were diagnosed as endometriosis by laparoscopy. The five ectopic en- dometria(two at stage II, two at stage III and one at stage IV) and five eutopic endometria were surgically excised. One-dimensional electrophoresis coupled with liquid chromatography and mass spectrometry was used to screen and identify differential proteins. Three differential bands in one-dimensional electrophoresis were resolved by liquid chromatography and mass spectrometry and 14 up-regulated proteins were identified, including collagen α-1, α-2, α-3(VI), α-1(XIV) chain, actin, annexin A2, EMILIN-1, ferritin light polypeptide variant, fucosyltransferase 10, myosin-9, protein S100-A9, KIAA1783 protein, and two hypothetical proteins. Our data provides a list of potential biomarkers for endometriosis. The identifications may be used to develop new diagnoses for endometriosis. Keywords Endometriosis; One-dimensional electrophoresis; Liquid chromatography; Mass spectrometry; Proteomics Article ID 1005-9040(2010)-01-070-05