Characterization of human trabecular bone across multiple length scales: A correlative approach combining X-ray tomography with LaserFIB and plasma FIB-SEM

Characterization of human trabecular bone across multiple length scales: A correlative approach combining X-ray tomography with LaserFIB and plasma FIB-SEM | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Characterization of human trabecular bone across multiple length scales: A correlative approach combining X-ray tomography with LaserFIB and plasma FIB-SEM Tengteng Tang, Travis Casagrande, Pardis Mohammadpour, William Landis, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4254247/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 16 Sep, 2024 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract Three-dimensional correlative multimodal and multiscale imaging is an emerging method for investigating the complex hierarchical structure of biological materials such as bone. This approach synthesizes images acquired across multiple length scales, for the same region of interest, to provide a comprehensive view of the material structure of a sample. Here, we develop a workflow for the structural analysis of human trabecular bone using a femtosecond laser to produce a precise grid to facilitate correlation between imaging modalities and identification of structures of interest, in this case, a single trabecula within a volume of trabecular bone. Through such image registration, high resolution X-ray microscopy imaging revealed fine architectural details, including the cement line and bone cell lacunae of the selected bone unit. The selected bone volume was exposed with a combination of manual polishing and site-specific femtosecond laser ablation and then examined with plasma focused ion beam-scanning electron microscopy. This reliable and versatile correlation approach has the potential to be applied to a variety of biological tissues and traditional engineered materials. The proposed workflow has the enhanced capability for generating highly resolved structural data for a better understanding of the architectural features of a material spanning its macroscopic to nanoscopic levels. Biological sciences/Biological techniques/Microscopy/Scanning electron microscopy Biological sciences/Biological techniques/Structure determination/Electron microscopy Biological sciences/Biological techniques/Imaging/3d reconstruction Biological sciences/Biological techniques/Imaging/X ray tomography Biological sciences/Biological techniques Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryMaterials.pdf Cite Share Download PDF Status: Published Journal Publication published 16 Sep, 2024 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 20 Jun, 2024 Reviews received at journal 16 Jun, 2024 Reviewers agreed at journal 28 May, 2024 Reviews received at journal 06 May, 2024 Reviewers agreed at journal 23 Apr, 2024 Reviewers invited by journal 21 Apr, 2024 Editor assigned by journal 19 Apr, 2024 Editor invited by journal 19 Apr, 2024 Submission checks completed at journal 17 Apr, 2024 First submitted to journal 11 Apr, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4254247","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":292571474,"identity":"afe9dc5d-bafd-4fc0-8e69-8b91515fd055","order_by":0,"name":"Tengteng 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