Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae

Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae Yutaro Ohe, Masaharu Hasebe, Yoshitaka Hamanaka, Shin G. Goto, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4442876/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 15 Jan, 2025 Read the published version in Journal of Comparative Physiology A → Version 1 posted 9 You are reading this latest preprint version Abstract Larvae of the flesh fly, Sarcophaga similis exhibit photoperiodic responses to control pupal diapause. Although the external coincidence model is applicable to S. similis photoperiodic responses, it remains unknown how circadian clock system integrates day-length information. To explore the mechanisms underlying the photoperiodic control of pupal diapause, we examined the neural circuitry involving circadian clock lateral neurons (LNs) and prothoracicotropic hormone (PTTH) neurons, the latter possibly controlling the ecdysteroid production. We also examined the photoperiodic effects on LN-fiber patterns in third-instar S. similis larvae. Immunohistochemistry showed that PERIOD and a neuropeptide pigment-dispersing factor (PDF) were co-localized in four cells per hemisphere, and we named these PDF-LNs of S. similis . Single-cell polymerase chain reaction of backfilled neurons from the ring gland showed that two pairs of pars lateralis neurons with contralateral axons (PL-c neurons) expressed ptth . Double labeling with immunohistochemistry and backfills revealed that PDF-immunoreactive varicose fibers projected in the proximity of fibers from PL-c neurons. However, pdf receptor was not but glutamate-gated chloride channel was expressed in most PL-c neurons. L-glutamate but not PDF acutely inhibited the spontaneous firing activity of PL-c neurons. The number of PDF-immunoreactive varicosities of PDF-LNs in the dorsal protocerebrum was significantly higher under short-day than that under long-day conditions in a time-dependent manner. These results suggest that PDF-LNs, PTTH neurons, and glutamate signaling form a potential neural circuity for the photoperiodic control of pupal diapause and that photoperiod modifies the connectivity strength between PDF-LNs and their post- or pre-neurons in the circuitry. PDF photoperiodism PTTH s-LNv varicosity Full Text Additional Declarations Competing interest reported. We disclosed that author Sakiko Shiga is an unpaid member of the advisory board of the Journal of Comparative Physiology A. Cite Share Download PDF Status: Published Journal Publication published 15 Jan, 2025 Read the published version in Journal of Comparative Physiology A → Version 1 posted Editorial decision: Revision requested 13 Jun, 2024 Reviews received at journal 12 Jun, 2024 Reviews received at journal 31 May, 2024 Reviewers agreed at journal 22 May, 2024 Reviewers agreed at journal 22 May, 2024 Reviewers invited by journal 21 May, 2024 Submission checks completed at journal 20 May, 2024 Editor assigned by journal 20 May, 2024 First submitted to journal 18 May, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4442876","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":308413525,"identity":"d71567ca-07d6-4382-9802-543972b1c90c","order_by":0,"name":"Yutaro Ohe","email":"","orcid":"","institution":"Osaka University","correspondingAuthor":false,"prefix":"","firstName":"Yutaro","middleName":"","lastName":"Ohe","suffix":""},{"id":308413526,"identity":"34c75056-b100-4752-ac2f-6e8f89300fb9","order_by":1,"name":"Masaharu Hasebe","email":"","orcid":"","institution":"Osaka University","correspondingAuthor":false,"prefix":"","firstName":"Masaharu","middleName":"","lastName":"Hasebe","suffix":""},{"id":308413527,"identity":"5f74d8ca-a901-4d24-ab5f-29e35370b6da","order_by":2,"name":"Yoshitaka Hamanaka","email":"","orcid":"","institution":"Osaka University","correspondingAuthor":false,"prefix":"","firstName":"Yoshitaka","middleName":"","lastName":"Hamanaka","suffix":""},{"id":308413528,"identity":"d17b56fc-07f7-4523-a7ad-aa23c529c46b","order_by":3,"name":"Shin G. 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Although the external coincidence model is applicable to \u003cem\u003eS. similis\u003c/em\u003e photoperiodic responses, it remains unknown how circadian clock system integrates day-length information. To explore the mechanisms underlying the photoperiodic control of pupal diapause, we examined the neural circuitry involving circadian clock lateral neurons (LNs) and prothoracicotropic hormone (PTTH) neurons, the latter possibly controlling the ecdysteroid production. We also examined the photoperiodic effects on LN-fiber patterns in third-instar \u003cem\u003eS. similis\u003c/em\u003e larvae. Immunohistochemistry showed that PERIOD and a neuropeptide pigment-dispersing factor (PDF) were co-localized in four cells per hemisphere, and we named these PDF-LNs of \u003cem\u003eS. similis\u003c/em\u003e. Single-cell polymerase chain reaction of backfilled neurons from the ring gland showed that two pairs of pars lateralis neurons with contralateral axons (PL-c neurons) expressed \u003cem\u003eptth\u003c/em\u003e. Double labeling with immunohistochemistry and backfills revealed that PDF-immunoreactive varicose fibers projected in the proximity of fibers from PL-c neurons. However, \u003cem\u003epdf receptor\u003c/em\u003e was not but \u003cem\u003eglutamate-gated chloride channel\u003c/em\u003e was expressed in most PL-c neurons. L-glutamate but not PDF acutely inhibited the spontaneous firing activity of PL-c neurons. The number of PDF-immunoreactive varicosities of PDF-LNs in the dorsal protocerebrum was significantly higher under short-day than that under long-day conditions in a time-dependent manner. These results suggest that PDF-LNs, PTTH neurons, and glutamate signaling form a potential neural circuity for the photoperiodic control of pupal diapause and that photoperiod modifies the connectivity strength between PDF-LNs and their post- or pre-neurons in the circuitry.\u003c/p\u003e","manuscriptTitle":"Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-05-31 14:46:36","doi":"10.21203/rs.3.rs-4442876/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2024-06-13T07:57:46+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-06-12T21:18:21+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-05-31T06:32:46+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"13177570736068390423354098393815441489","date":"2024-05-23T00:22:54+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"25818891901764471263529369583549753781","date":"2024-05-22T07:00:52+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-05-22T01:17:38+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-05-20T11:25:24+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-05-20T11:25:24+00:00","index":"","fulltext":""},{"type":"submitted","content":"Journal of Comparative Physiology A","date":"2024-05-19T03:44:27+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"journal-of-comparative-physiology-a","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"jcpa","sideBox":"Learn more about [Journal of Comparative Physiology A](http://link.springer.com/journal/359)","snPcode":"359","submissionUrl":"https://submission.nature.com/new-submission/359/3","title":"Journal of Comparative Physiology A","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"87a99a9c-c207-4bb4-9729-13be56d0ba48","owner":[],"postedDate":"May 31st, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2025-01-20T16:07:43+00:00","versionOfRecord":{"articleIdentity":"rs-4442876","link":"https://doi.org/10.1007/s00359-024-01729-y","journal":{"identity":"journal-of-comparative-physiology-a","isVorOnly":false,"title":"Journal of Comparative Physiology A"},"publishedOn":"2025-01-15 15:58:01","publishedOnDateReadable":"January 15th, 2025"},"versionCreatedAt":"2024-05-31 14:46:36","video":"","vorDoi":"10.1007/s00359-024-01729-y","vorDoiUrl":"https://doi.org/10.1007/s00359-024-01729-y","workflowStages":[]},"version":"v1","identity":"rs-4442876","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4442876","identity":"rs-4442876","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}