Increased diversity of beneficial rhizobia enhances faba bean growth

Increased diversity of beneficial rhizobia enhances faba bean growth | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Increased diversity of beneficial rhizobia enhances faba bean growth Marcela Mendoza-Suarez, Turgut Akyol, Marcin Nadzieja, Stig Andersen This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4371008/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 12 Dec, 2024 Read the published version in Nature Communications → Version 1 posted You are reading this latest preprint version Abstract Legume-rhizobium symbiosis provides a sustainable nitrogen source for agriculture. Nitrogen fixation efficiency depends on both legume and rhizobium genotypes, but the implications of their interactions for plant performance in environments with many competing rhizobium strains remain unclear. Here, we let 399 Rhizobium leguminosarum bv. viciae strains compete for nodulation of 212 faba bean genotypes. We find that the strains can be categorised based on by their nodule occupancy profiles into groups that show distinct competitive interactions and plant growth-promoting effects. Further, we show that the diversity of strains occupying root nodules affects plant growth and is under plant genetic control. These insights provide a basis for re-designing rhizobium inoculation and plant breeding strategies to enhance symbiotic nitrogen fixation in agriculture. Biological sciences/Microbiology/Applied microbiology Biological sciences/Microbiology Nitrogen fixation symbiosis legume rhizobium faba bean plant growth-promoting rhizobacteria (PGPR) Full Text Additional Declarations There is NO Competing Interest. Supplementary Files nrreportingsummaryNCOMMS2426864T.pdf Reporting Summary Cite Share Download PDF Status: Published Journal Publication published 12 Dec, 2024 Read the published version in Nature Communications → Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4371008","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":305969915,"identity":"8d26c9f3-2cdb-4939-8fb5-92a2e0f7ae02","order_by":0,"name":"Marcela Mendoza-Suarez","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAyklEQVRIiWNgGAWjYNCCAgY5BgkIk3EGcVoMGIxJ15LYQLQW3fazDz98MLBLXzu7/QLDzzYG2ZkNBLSYnUk3lpxhkJy77c6ZAsbeNgbj2YRsMTuQxsbMY8Ccu+1GTgIDbxtD4jyCWs4/Y2P+Y1CfbgbUwviXKC03gLYwGBxOMLuRfoAZZAthh914xizZY3DcEOgXhsMy5ySMCXv/fBrjhx8V1fJmt9sfPnxTZiM74wAhaxCAxwCoWIJ49UDA/oAk5aNgFIyCUTByAADNT0F2yiczaAAAAABJRU5ErkJggg==","orcid":"https://orcid.org/0000-0003-3419-1362","institution":"Aarhus University","correspondingAuthor":true,"prefix":"","firstName":"Marcela","middleName":"","lastName":"Mendoza-Suarez","suffix":""},{"id":305969916,"identity":"4f21102c-31d0-48c5-b86f-3d0a68f8a995","order_by":1,"name":"Turgut Akyol","email":"","orcid":"","institution":"Aarhus University","correspondingAuthor":false,"prefix":"","firstName":"Turgut","middleName":"","lastName":"Akyol","suffix":""},{"id":305969917,"identity":"181b64e6-afdb-4e47-aa7f-012c937d0e7d","order_by":2,"name":"Marcin Nadzieja","email":"","orcid":"","institution":"Aarhus University","correspondingAuthor":false,"prefix":"","firstName":"Marcin","middleName":"","lastName":"Nadzieja","suffix":""},{"id":305969918,"identity":"877f90b0-3e4b-4b3e-8d76-f1d8f6f1f8fd","order_by":3,"name":"Stig Andersen","email":"","orcid":"https://orcid.org/0000-0002-1096-1468","institution":"Aarhus University","correspondingAuthor":false,"prefix":"","firstName":"Stig","middleName":"","lastName":"Andersen","suffix":""}],"badges":[],"createdAt":"2024-05-05 09:05:07","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4371008/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4371008/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s41467-024-54940-5","type":"published","date":"2024-12-12T05:00:00+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":71313229,"identity":"bb3682ce-7eea-4e16-97c4-3c8ac44d5176","added_by":"auto","created_at":"2024-12-13 08:05:35","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":3591767,"visible":true,"origin":"","legend":"","description":"","filename":"20240421ManuscriptIncreaseddiversityofbeneficialrhizobiaenhancesfababeangrowth.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4371008/v1_covered_5924aa8c-36bc-4c21-acad-a3976de1705e.pdf"},{"id":57056469,"identity":"d2d4289b-bc4a-4df4-acaf-5a3104f34e04","added_by":"auto","created_at":"2024-05-24 04:54:05","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":343206,"visible":true,"origin":"","legend":"Reporting Summary","description":"","filename":"nrreportingsummaryNCOMMS2426864T.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4371008/v1/5f7d36ae5ca5f29bc6a46e55.pdf"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"Increased diversity of beneficial rhizobia enhances faba bean growth","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"nature-portfolio","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"","title":"Nature Portfolio","twitterHandle":"","acdcEnabled":false,"dfaEnabled":false,"editorialSystem":"ejp","reportingPortfolio":"","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Nitrogen fixation, symbiosis, legume, rhizobium, faba bean, plant growth-promoting rhizobacteria (PGPR)","lastPublishedDoi":"10.21203/rs.3.rs-4371008/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4371008/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Legume-rhizobium symbiosis provides a sustainable nitrogen source for agriculture. 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