Systematic barcoding of 12 major medicinal herbs in the Apiaceae family

Systematic barcoding of 12 major medicinal herbs in the Apiaceae family | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Systematic barcoding of 12 major medicinal herbs in the Apiaceae family Ju-Young Ahn, Jee Young Park, Hyun-Seung Park, Ji-Hyeok Lee, Seung Kyu Kim, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8241605/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Medicinal plants of the Apiaceae family often exhibit high morphological similarity, making accurate species identification challenging and leading to frequent cases of adulteration or substitution. To establish a systematic barcoding framework, we simultaneously assembled the chloroplast genomes (plastomes) and 45S nuclear ribosomal DNA (nrDNA) sequences of 12 major medicinal herb species and performed comparative genomic analyses to identify interspecific variations in single-nucleotide polymorphisms (SNPs), insertions/deletions, nucleotide diversity (π), and simple sequence repeats (SSRs). Based on these genomic insights, seven high-resolution melting (HRM) markers were developed, which generated species-specific melting curve profiles and enabled unambiguous identification and discrimination of all 12 taxa. Compared with conventional DNA barcoding, HRM demonstrated advantages in cost-effectiveness, speed, and suitability for high-throughput analysis. Collectively, our study provides genomic resources and molecular tools that establish a comprehensive barcoding system for 12 medicinal herbs in the Apiaceae family, facilitating reliable species identification, genetic diversity assessment, and phylogenetic research, while contributing to the conservation and safe utilization of these plants. Apiaceae Medicinal plants Plastome High Resolution Melting (HRM) Systematic barcoding Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryFile.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8241605","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":557506192,"identity":"11ee50e7-c0a3-40cd-a8ed-11c7a7dd5d21","order_by":0,"name":"Ju-Young Ahn","email":"","orcid":"","institution":"Seoul National University","correspondingAuthor":false,"prefix":"","firstName":"Ju-Young","middleName":"","lastName":"Ahn","suffix":""},{"id":557506193,"identity":"d8270a67-3719-48c8-87fa-9551b718a008","order_by":1,"name":"Jee Young Park","email":"","orcid":"","institution":"Seoul National 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