Effect of Bushen Wenyang Huayu on Expression of HIF-1α and GLUT-1 in Rat Model of Endometriosis

Objective:To explore the mechanism of Bushen Wenyang Huayu(BSWYHY) on endometriosis(EMs).Method: The peritoneal type endometriosis in SPF-grade female rats was established.They were then divided into 4 groups: BSWY high dose group(16),BSWYHY low dose group(16),danazol group(16),model group(17),and control group(12).Immunohistochemistry was performed to examine the expression of HIF-1α and GLUT-1 in the ectopic endometrial tissues of the EMs model rats before and after treatment,and the volume change in endometriotic lesions before and after treatment was compared.Result:After treatment with high dose and low dose of BSWY and danazol,volumes of ectopic lesions were reduced obviously(P 0.05),and the volume of the high dose group demonstrated the most notable reduction(P 0.01).The expression of HIF-1α and GLUT-1 in ectopic EMs of the model group is significantly higher than that in eutopic endometrium in the control group(P 0.01).The BSWYHY high and low dose group and the danazol group were able to inhibit the high expression of HIF-1α and GLUT-1 substantially(P 0.01),with the high dose group as the best,followed by the low dose anddanazol group(P 0.05).Conclusion: BSWY can be an effective measure for reducing the high expression of HIF-1α and GLUT-1 for the treatment of EMs.