Probing Chirality of Crystals using Electron Paramagnetic Resonance (EPR) spectroscopy

One of the most challenging tasks in analytical chemistry is the determination of the chirality (identification of an enantio-meric composition) in solids mainly because of the strict requirements of the pharmaceutical industry for enantiomerically pure drugs. Although there are a few methods available to accomplish enantio-differentiation in solids, for example: X-ray diffraction (XRD), differential scanning calorimetry (DSC), CD spectroscopy, and low-frequency (LF) Raman spectroscopy, this is still very challenging. In this work, we have developed a new method to measure the chirality of crystals, based on electron paramagnetic resonance (EPR) spectroscopy of chiral crystals doped with Cu 2+ as the EPR active ion. Here, we demonstrate our approach using a model system of L- and DL-Histidine crystals doped with Cu 2+ . We show that EPR measurements of the Cu 2+ -doped Histidine crystals can accurately determine the chirality and enantiomeric composition of the crystals. We present a very preliminary example of this technique, and we hope that in the future it will be possible to refine and develop this method for many other chiral organic crystal systems.