Occupational polycyclic aromatic hydrocarbons (PAHs) exposure and aging trajectory : Epidemiologic evidence from a cohort of Chinese coke oven workers (2019-2023)

Occupational polycyclic aromatic hydrocarbons (PAHs) exposure and aging trajectory : Epidemiologic evidence from a cohort of Chinese coke oven workers (2019-2023) | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Occupational polycyclic aromatic hydrocarbons (PAHs) exposure and aging trajectory : Epidemiologic evidence from a cohort of Chinese coke oven workers (2019-2023) Yidong Wang, Shuangxi Geng, Wenyu Wang, Lijun Yuan, Jisheng Nie, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7945721/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 31 Jan, 2026 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract Background: Long-term effects of occupational polycyclic aromatic hydrocarbons (PAHs) on biological aging are unclear. Methods: A prospective cohort study was conducted from 2019 to 2023, involving 610 coke oven workers and 454 control workers from a water treatment plant. Biological age was calculated using the Klemera and Doubal method (KDM-BA) based on 12 clinical biomarkers, and aging acceleration (KDM-Accel) was derived. Group-based trajectory modeling (GBTM) was employed to identify distinct aging trajectories over five time points. The associations between eleven urinary mono-hydroxylated PAH metabolites (measured via HPLC-MS) and both KDM-Accel and aging trajectories were assessed using multiple linear regression and multivariable logistic regression, respectively. Results: GBTM identified three distinct aging trajectories: slow aging (14.86%), moderately accelerated aging (59.14%), and rapidly accelerated aging (26%). Higher concentrations of urinary Σ-OHPAHs (sum of all metabolites), 1-hydroxypyrene (1-OHPyr), and 2-hydroxyphenanthrene (2-OHPhe) were significantly associated with increased KDM-Accel. A natural log-unit increase in Σ-OHPAHs and 1-OHPyr was associated with a 0.029-year and 0.028-year increase in KDM-Accel, respectively. Workers in the highest tertile (T3) of Σ-OHPAHs exposure had a 61.2% increased odds (OR = 1.612, 95% CI: 1.093–2.376) of being in the rapidly accelerated aging trajectory compared to those in the lowest tertile (T1). Similar positive dose-response relationships were observed. Conclusion: Occupational PAH exposure accelerates biological aging ; enhanced protection and early intervention are warranted. Health sciences/Biomarkers Earth and environmental sciences/Environmental sciences Health sciences/Health care Health sciences/Medical research Health sciences/Risk factors Polycyclic aromatic hydrocarbons Occupational exposure Biological age Aging trajectory Prospective cohort study Figures Figure 1 Figure 2 Figure 3 Figure 4 1. Introduction Polycyclic aromatic hydrocarbons (PAHs), a major component of air pollutants, originate from the incomplete combustion of organic matter. These compounds, composed of multiple fused benzene rings, form hydrocarbon substances with planar, angular, or cluster structures 1 ..PAHs have been associated with a range of adverse health effects, including cardiovascular diseases 2 , cognitive dysfunction 3 , cancer 4 , metabolic abnormalities 5 , and increased mortality 6 . Furthermore, the consequences related to PAHs exposure are closely linked to the aging process. Epidemiological studies have indicated that PAHs exposure is correlated with various aging biomarkers. These include telomere shortening 7 , changes in DNA methylation patterns, increased mitochondrial DNA content 8 , and higher skin aging scores 9 . Such findings suggest that PAHs may play a role in accelerating both epigenetic and cellular aging processes 10 , 11 . However, it is important to note that the aforementioned aging-related biomarkers may have certain limitations. In recent years, the quantification of biological aging in conjunction with standard clinical parameters has been proven to be one of the most accurate methods for predicting diseases and mortality 12 , 13 . This approach is also becoming a key method for understanding how environmental exposures and interventions influence the risk of chronic diseases 14 . Unlike mere chronological aging, biological aging reflects the cumulative cellular and molecular damage that leads to functional decline 15 . Recent analysis of the National Health and Nutrition Examination Survey (NHANES) database has revealed a correlation between exposure to mixed PAHs and increased aging 16 . Among these, 2-naphthol has been identified as a key component of PAHs associated with aging. Nonetheless, the precise relationship between PAHs exposure and biological age trajectories remains unclear. Specifically, it is not well understood how PAHs affect aging-related pathways in the early stages of exposure or to what extent they influence the aging process. Additionally, when compared to the general population residing outside industrial areas, coal miners and coking plant workers are exposed to PAHs for longer durations and at higher intensities within more complex environmental mixtures. This chronic exposure may not only increase the risk of age-related diseases but also potentially accelerate biological aging itself, leading to the earlier onset of aging-related functional decline 3 , 17 , 18 . Therefore, this study was designed as a prospective cohort study with the aim of assessing the impact of PAHs on biological age and aging trajectories. By developing a biological aging prediction model based on newly established biomarkers of biological aging and constructing aging trajectories, this study seeks to provide valuable insights into the long-term effects of PAHs exposure. The results of this research will offer scientific evidence for early disease intervention and risk stratification through the assessment of biological aging trajectories. Moreover, the findings will serve as an epidemiological basis to guide occupational workers in implementing effective protective measures to mitigate the health risks associated with PAHs exposure. 2. Material and methods 2.1 Study participants This study used cluster sampling to build a professional worker cohort, recruiting 610 coke oven workers and 454 from a water treatment plant in Shanxi Province in 2019. Follow-ups were done in 2020–2023. Participants were informed of the study’s purpose and procedures, advised to maintain a light diet, and required to fast for at least 12 hours before sample collection. At enrollment, each participant completed a questionnaire on demographics, lifestyle, and medical history, conducted face-to-face by trained investigators using a standardized "Health Survey Form". For aging trajectory analysis, 763 coking plant cohort participants aged ≥ 20 with baseline measurements in 2019 and four aging assessments (KDM-BA) were included.After excluding those with missing PAH exposure or demographic data, 673 subjects remained for longitudinal analysis to explor e the association between PAHs exposure and aging trajectories. The flowchart is shown in Fig. 1 a.We screened 55,720 occupational examinees in Shanxi, 2019. Excluding non-frontline workers and those aged 80 years or with missing biomarkers yielded 27,806 participants (Fig. 1 b). The occupational cohort served as the training set and the coke oven cohort as the test set for a biological aging prediction model.This study was approved by the Medical Ethics Committee of the Shanxi Medical University and all participants signed informed consent form.The serial number is 2020GLL037. 2.2 Baseline data collection 2.2.1 General questionnaire collection The pre-enrollment questionnaire primarily covered seven sections: worker demographics (age, gender, ethnicity, height, weight, marital status, etc.), residential info, dietary habits (vegetables, fruits, meats, etc.), lifestyle habits (smoking, drinking, etc.), occupational history, medical history, and sleep patterns. Smokers are defined as those smoking ≥ 1 cigarette/day for ≥ 6 months; drinkers as ≥ 3 times/week for ≥ 1 year. Marital status includes single, married, etc. BMI is calculated as weight/height² (kg/m²). Each survey took ≤ 15 minutes to ensure quality. 2.2.2 Routine physical examination All participants underwent a health examination, with blood samples and morning urine collected. Testing was done within 24 hours, including biochemical tests, blood pressure, ECG, and ultrasound. 2.3 Determination of urinary PAHs metabolites During physical exams, professionals collected 50 mL urine samples using sterile cups. Samples were promptly transported to the lab, measured for specific gravity, and sub-packed into 5-mL cryotubes, then frozen at -80°C. High-performance liquid chromatography-mass spectrometry (HPLC-MS) measured 11 PAH metabolites, including 2-OHNAP, 10-OHNAP, etc. Detailed procedures and quality control data are in previous studies(Table S1 ). 2.4 Selection of biomarkers Biomarker selection considered their role in aging, prior aging study applications, and availability in the occupational worker dataset, along with their Pearson correlation with chronological age. The 38 biomarkers analyzed fall into seven categories: metabolic function (BMI, blood sugar, etc.), cardiac function (blood pressure, heart rate), pulmonary function (FVC, FEV1, FEV1/FVC), renal function (urine pH, UA, etc.), liver function (ALT, AST), immune function (lymphocyte count, etc.), and blood cell counts (white blood cell count, etc.). Pearson correlation assessed the relationship between these biomarkers and age (Table S2). Notably, platelet volume and count, hemoglobin, red blood cell count, hematocrit, systolic and diastolic blood pressure demonstrated high intercorrelation (r > 0.7). Following Klemera and Doubal's dimensionality reduction approach, a single clinically relevant biomarker was systematically selected from each correlated parameter group as a representative indicator (e.g., platelet count for platelets, red blood cell count for erythrocytes, systolic blood pressure for blood pressure).Ultimately, 12 biomarkers were selected for biological age calculation, all with correlation coefficients > 0.1. These included ALT, RBC, HDL, TC, MCV, MCHC, RDW, PLT, LYC, BUR, and SBP. 2.5 Statistical analysis Data from questionnaires and standardized instruments were entered into a database using EpiData 3.1 software, with subsequent data cleaning performed in SPSS 25. Descriptive statistics were used to summarize the baseline characteristics of the study population during their initial physical examination. Socio-demographic characteristics were presented as mean ± standard deviation (X ± S) or frequency (percentage) (N%). Levels of polycyclic aromatic hydrocarbon hydroxy metabolites in urine were transformed using natural logarithm (Ln transformation) to achieve approximate normality for continuous variables, or categorized into three levels (T1, T2, T3) based on tertiles for categorical variables. Categorical variables were compared using chi-square tests, while continuous variables were analyzed with t-tests to assess differences in characteristics among different aging trajectory groups. Spearman correlation analysis was applied to evaluate the correlations among the 11 polycyclic aromatic hydrocarbons. Biological age (KDM-BA) and biological aging acceleration (KDM-Accel) were precisely measured according to the method proposed by Klemera and Doubal. The prediction accuracy was assessed by calculating the Pearson correlation coefficient (R) and root mean square error (RMSE) between chronological age and KDM-BA. Moreover, to account for the effect of chronological age (CA), KDM-BA acceleration (KDM-Accel) was defined as the residual from the linear regression of KDM-BA on CA. The training dataset demonstrated a near-perfect Spearman's rank correlation (ρ = 0.99) between chronological age and biological age in the occupational worker cohort, with RMSE = 0.205 years and MAE = 0.165 years (Fig. S2a). In the testing dataset focusing on the coking plant cohort, the correlation coefficient R2 was 0.99, with RMSE of 0.234 and MAE of 0.175,as seen in supplemental Fig. 2b. Both indicated good model predictability, verifying the effectiveness of the predictor. KDM-Accel is planned to be included as a biological aging indicator in subsequent analyses. To explore the correlations between different hydroxy polycyclic aromatic hydrocarbon (OH-PAH) metabolites and the aging process, a multiple linear regression model was constructed, with the aging acceleration indicator (KDM-Accel) as the response variable and various OH-PAH metabolites (as continuous variables) as explanatory variables. The model adjusted for covariates including age, gender, education level, smoking status, alcohol consumption, tea consumption, exercise status, monthly income, shift work status, and other polycyclic aromatic hydrocarbon metabolites in urine. For missing covariate data, a dummy variable encoding method was used for missing categorical variables, and the median of continuous variables was used as a substitute for estimation. Additionally, restricted cubic spline models (RCS) were applied to analyze the dose-response relationship between polycyclic aromatic hydrocarbon exposure and the biological aging rate of workers. KDM-Accel was calculated at five specific time points. Group-based trajectory modeling (GBTM) based on KDM-Accel was used to identify different aging trajectories at these five time points. The specific study design flow chart is shown in Fig.S2. The GBTM method is based on the assumption that the study population consists of different groups defined by individual developmental trajectories, and this process was implemented in SAS PROC TRAJ, with aging trajectories determined empirically. The number of subgroups of the biological aging index and the functional model for each group were determined by the following principles: (1) Bayesian Information Criterion (BIC), (2) average posterior probability of members (each biological aging trajectory class ≥ 0.7), and (3) reasonable distribution of study subjects in trajectory classes. A series of possible parameters for the GBTM model were considered, including fitting cubic, quadratic, and linear terms, with variations in one to six trajectory groups. The best-fitting trajectory model (3Group) was ultimately selected. These different aging trajectories consist of individuals with similar aging acceleration patterns, demonstrating the dynamic and heterogeneous process of aging. Specific parameter selection details are shown in Tables S3-S6 and Fig.S3. This study further employs multiple logistic regression analysis to assess the association between the tertiles of exposure levels to polycyclic aromatic hydrocarbons (PAHs) and aging trajectories .The lowest tertile was used as the reference exposure level, and the slow-aging trajectory as the reference outcome category. Odds ratios (ORs) and their 95% confidence intervals (CIs) were calculated. Trend tests were performed by assigning the median of each tertile to the index and including it as a continuous variable in the model. Additionally, these indicators were included in the analysis as continuous variables (per 10-unit increase). To explore the impact of gender differences on the relationship between polycyclic aromatic hydrocarbons and aging trajectories, stratified analysis by gender was conducted. Multinomial logistic regression models were used to estimate ORs and 95% CIs, and further explored the relationships between the 11 polycyclic aromatic hydrocarbons and aging trajectories. All analyses were conducted using SAS 9.7 (SAS Institute, Cary, NC), and figures were drawn using R 4.3.2. All p-values were two-sided. A p-value < 0.05 was considered statistically significant.The technology roadmap of this study is shown in Fig.S4. 3. Results 3.1 Basic characteristics Among the 673 participants in the longitudinal study, the average age was 44.71 ± 7.32 years, and 89.45% were male(Table S7). Over half of the workers had an educational level above high school. Approximately 39.23% and 59.14% of the study subjects never smoked or drank alcohol, respectively, and 426 individuals (63.3% of the total) consumed tea. However, most subjects did not engage in healthy physical activities. Compared with the control group, coking plant workers were younger and had a higher proportion of males (91.91% vs. 86.21%, P = 0.017), and a smaller proportion of those who often participated in physical exercise (32.64% vs. 41.03%, P 0.05). Although there was no significant difference in KDM-Accel between the two groups, the average value was higher in the coking plant group than in the water treatment plant group. The distribution of OH-PAHs in the urine of workers have been adjusted for urine specific gravity(Table S7). The detection rates of the 11 OH-PAHs metabolites were all above 75%. The geometric mean of OH-PAHs metabolites was calculated. The highest concentration of OH-PAHs metabolites was 2-OHNap (0.94 µg/L), and the lowest was 9-OHBap (0.07 µg/L). In the control group, the concentrations of OH-PAHs metabolites in the urine of water treatment plant workers were 9-OHPhe (0.37 µg/L) and 3-OHChr (0.04 µg/L). The levels of OH-PAHs in the urine of coking plant workers were consistently higher than those in the control group from the water treatment plant. GBTM identified three potential categories of biological aging trajectories (Fig. 2 a).Approximately 14.86% of the study subjects were classified into the slow aging trajectory group, all with negative KDM-Accel values, indicating that their biological age was generally younger than their chronological age across the five measures. About 59.14% were placed in the moderately accelerated aging trajectory group, showing moderate levels of accelerated aging initially. Individuals in this group had significantly higher urinary PAHs metabolite concentrations at baseline than those in the slow aging trajectory group. The remaining 26% were in the highly accelerated aging group, showing a high rate of accelerated aging across all four measures. The age of this group was 45.41 ± 7.12 years, and the concentration of PAHs metabolites in urine was the highest(Table 1 ). Figure 2 b displays the pairwise Spearman's rank correlations ( ρ ) among 11 polycyclic aromatic hydrocarbons (PAHs), with values ranging from 0.04 to 0.75. Specifically, 2-OHNap was moderately correlated with 2-OHPhe and 1-OHNap (r = 0.52, 0.57), and strongly correlated with 3-OHChr and 9-OHBap (r = 0.75). Sensitivity analysis was subsequently conducted, and aging trajectories were constructed separately for the coking plants and power plants using the GBTM method. The results showed that the trajectories of the two plants were homogeneous and showed increasing trends, with coking plants exhibiting a faster growth rate, a larger slope, and a higher KDM-Accel value than the control group (Fig. 2 c and 2 d). Table 1 Basic Characteristics of coke oven workers (n = 673) variable Total population n = 673 Aging trajectories P value Slow aging group Moderately accelerated aging group Highly accelerated aging group n = 100 n = 398 n = 175 Age (years) 44.71 ± 7.32 44.61 ± 6.77 44.43 ± 7.53 45.41 ± 7.12 0.495 gender < 0.001 man 602(89.45) 77(77.00) 357(89.70) 168(96.00) woman 71(10.55) 23(23.00) 41(10.30) 7(4.00) Educational attainment (years, N%) < 0.001 1–9 176(26.15) 28(28.00) 100(25.13) 48(27.43) 10–12 421(62.56) 61(61.00) 247(62.06) 113(64.57) ≥ 13 76(11.29) 11(11.00) 51(12.81) 14(8.00) marital status (Yes, N%) 0.197 Yes 636(94.50) 93(93.00) 373(93.72) 170(97.14) No 37(5.50) 7(7.00) 25(6.28) 5(2.86) Smoking (Yes, N%) 409(60.77) 56(56.00) 254(63.82) 99(56.57) 0.150 Alcohol consumption (Yes, n%) 275(40.86) 47(47.00) 156(39.20) 72(41.14) 0.364 Tea drinking condition (Yes, n%) 426(63.30) 59(59.00) 254(63.82) 113(64.57) 0.618 Exercise status 0.676 never 186(27.64) 23(23.00) 115(28.89) 48(27.43) Occasionally 243(36.11) 38(38.00) 137(34.42) 68(38.86) often 244(36.26) 39(39.00) 146(36.68) 59(33.71) Night shift (yes, n%) 467(69.60) 70(70.00) 282(71.21) 115(65.71) 0.418 KDM (years) 44.61 ± 7.33 44.28 ± 6.77 44.30 ± 7.55 45.48 ± 7.12 0.127 kdm_advance (years old) -0.11 ± 0.17 -0.33 ± 0.12 -0.13 ± 0.12 0.07 ± 0.11 < 0.001 Urine PAHs metabolites (µg/L) 2-OHNap 0.68 ± 1.22 0.49 ± 0.77 0.67 ± 1.03 0.84 ± 1.71 0.544 1-OHNap 0.25 ± 1.16 0.17 ± 0.35 0.27 ± 1.39 0.27 ± 0.85 0.723 3-OHFlu 0.09 ± 0.15 0.07 ± 0.08 0.09 ± 0.13 0.11 ± 0.19 0.866 2 OHFlu 0.15 ± 0.18 0.13 ± 0.12 0.15 ± 0.16 0.16 ± 0.24 0.408 2-OHPhe 0.28 ± 0.47 0.19 ± 0.22 0.25 ± 0.35 0.39 ± 0.74 0.046 9-OHPhe 0.42 ± 0.55 0.35 ± 0.29 0.43 ± 0.50 0.45 ± 0.75 0.151 1-OHPhe 0.10 ± 0.16 0.09 ± 0.12 0.10 ± 0.13 0.11 ± 0.24 0.345 1-OHPyr 0.20 ± 0.25 0.14 ± 0.15 0.21 ± 0.26 0.23 ± 0.29 0.049 3-OHChr 0.06 ± 0.09 0.04 ± 0.04 0.05 ± 0.07 0.07 ± 0.13 0.627 6-OHChr 0.08 ± 0.08 0.07 ± 0.05 0.09 ± 0.07 0.09 ± 0.10 0.272 9-OHBap 0.06 ± 0.09 0.05 ± 0.05 0.06 ± 0.07 0.07 ± 0.12 0.473 Ʃ-OH PAHs 2.19 ± 2.67 1.65 ± 1.25 2.17 ± 2.46 2.57 ± 3.52 0.247 P < 0.05; The continuous data were expressed as x ± s, and the statistical differences were compared by ANOVA. The numerical data were expressed as n (%), and the chi-square test was used to compare the statistical differences. 3.2 Association between occupational exposure and biological aging of workers exposed to polycyclic aromatic hydrocarbons This study first explored the linear correlation between PAHs and the biological aging indicator (KDM-Accel). Using the natural logarithm-transformed PAH metabolites as continuous variables, we performed multiple linear regression analyses.Table 2 shows the results of the multiple linear regression model after controlling for covariables. The analysis indicates that exposure to 1-OHPyr and 2-OHPHE significantly increases workers' biological age .Conversely, 2-OHFlu shows a negative correlation with biological age. Specifically, a one-unit natural log increase in 1-OHPYR concentration is associated with a 0.028-year increase in KDM-Accel; a similar increase in 2-OHPHE concentration corresponds to a 0.017-year rise. Conversely, a one-unit natural log increase in 2-OHNAP concentration is linked to a 0.026-year decrease in KDM-Accel. After further covariate adjustment, these effects weakened but remained statistically significant (P < 0.05). For total PAH metabolites, Model 1 adjusted for age, sex, and education. Results show that a one-unit natural log increase in Σ-OHPAHs is associated with a 0.029-year increase in KDM-Accel. In Model 2, further adjustment for smoking, drinking, tea consumption, exercise, monthly income, and night shift status shows a 0.032-year increase in KDM-Accel per one-unit natural log increase in Σ-OHPAHs. Table 2 Relationship between urinary OH-PAHs metabolites and occupational workers' biological aging index KDM-Accel In urine OH-PAHs Metabolites Multiple linear regression results β (95% CI) Model one P-value Model two P-value Ʃ-OHPAHs a 0.029(0.013,0.046) < 0.001 0.032(0.015,0.049) < 0.001 2-OHNAP b 0.003(-0.007,0.013) 0.518 0.005(-0.005,0.015) 0.347 1-OHNAP b 0.001(-0.013,0.015) 0.862 0.001(-0.013,0.015) 0.860 3-OHFLU b 0.007(-0.005,0.018) 0.247 0.007(-0.005,0.018) 0.264 2-OHFLU b -0.026(-0.040,-0.011) < 0.001 -0.022(-0.037,-0.007) 0.004 2-OHPHE b 0.017(0.005,0.028) 0.005 0.014(0.002,0.026) 0.019 9-OHPHE b 0.003(-0.0169,0.022) 0.736 0.005(-0.015,0.025) 0.608 1-OHPHE b -0.001(-0.014,0.011) 0.836 -0.003(-0.015,0.009) 0.615 1-OHPYR b 0.028(0.011,0.045) 0.001 0.028(0.011,0.045) 0.001 3-OHChr b -0.012(-0.029,0.005) 0.175 -0.011(-0.028,0.006) 0.209 6-OHChr b -0.001(-0.014,0.013) 0.972 -0.001(-0.014,0.013) 0.900 9-OHBap b 0.013(0.002,0.024) 0.020 0.012(0.001,0.023) 0.042 Model 1:adjusted for age, sex, and education level. b.adjusted for age, sex, education level, and other urinary OH-PAHs metabolites. Model 2: adjusted for age, gender, education level, smoking status, drinking status, tea consumption status, exercise status, monthly income, and night shift status. b adjusted for age, gender, education level, smoking status, drinking status, tea consumption status, exercise status, monthly income, night shift status, and other urinary OH-PAHs metabolites. Bold indicates P < 0.05. Log-transformed concentrations of 11 individual PAH metabolites and Σ-OHPAHs were partitioned into tertiles based on their 33rd (P33) and 66th (P66) percentiles, defining three exposure strata: Group 1, Group 2,and Group 3.Categorical variables were included in the model for analysis. After adjusting for covariates, a generalized linear regression model was used to analyze the relationship between hydroxy-PAHs levels and KDM-Accel, as shown in Fig. 3 . The model was adjusted for age, sex, education, smoking status, alcohol consumption, tea consumption, exercise status, monthly income, night shift status, and other urinary PAH metabolites. The analysis reveals that compared to Group 1, Group 3 of Σ-OHPAHs is associated with a 0.033-year increase in the workers' biological aging indicator. Trend testing indicates a significant upward trend in the biological aging indicator with increasing urinary Σ-OHPAHs levels (P_trend = 0.034). For 1-OHPyr, Group 3 versus Group 1 is associated with a 0.063-year increase in the biological aging indicator (OR = 0.063; 95%CI: 0.0215 ~ 0.1048). Trend testing also shows a significant upward trend in the biological aging indicator with increasing urinary 1-OHPyr levels (P_trend = 0.006). This study used restricted cubic splines to analyze the dose-response relationship between workers' Σ-OHPAHs and the KDM-Accel. Using the median concentration of Σ-OHPAHs as the reference point, three knots (P 10 , P 50 , and P 90 ) were selected for analysis, with results shown in Fig. 4 a. A significant dose-response relationship was found between Σ-OHPAHs and the KDM-Accel (P overall < 0.001). As urinary Σ-OHPAHs concentrations increase, workers' biological aging rates also increase in a linear dose-response manner. Additionally, using the median concentration of 1-OHPYR as the reference point and selecting three knots (P 10 , P 50 , and P 90 ) for analysis, the results (Fig. 4 b)indicate a significant dose-response relationship between 1-OHPYR levels and the KDM-Accel (P overall < 0.001). As urinary 1-OHPYR concentrations increase, workers' biological aging rates also increase, exhibiting a non-linear dose-response relationship. 3.3 Association between occupational exposure and biological aging trajectories in workers exposed to polycyclic aromatic hydrocarbons 3.3.1 Basic information about workers The longitudinal data included 1064 workers who participated in the baseline survey and 673 workers who completed the follow-up. Table 3 compares the baseline information of 1064 workers with 673 workers included in the cohort study. The results showed that there were no statistically significant differences in age, sex, marital status, smoking status, drinking status, tea drinking status and night shift status between the two groups. Table 3 Comparison of basic information between all workers at baseline (n = 1064) and workers included in the cohort (n = 673). Baseline: All workers (n = 1064) Cohort Inclusion Study Workers (n = 673) P value Age (years) 45.35 ± 7.09 44.71 ± 7.32 0.075 gender 0.647 man 959(90.13) 602(89.45) woman 105(9.87) 71(10.55) Marital status (yes, N%) 0.951 Tie the knot 1007(94.73) 638(94.80) Not married 56(5.27) 35(5.20) Smoking (Yes, N%) 671(63.06) 409(60.77) 0.337 Alcohol consumption (yes, n%) 447(42.05) 275(40.86) 0.624 Tea drinking status (yes, n%) 687(64.69) 425(63.15) 0.515 Night shift (yes, n%) 717(67.58) 467(69.60) 0.379 Note: Bold represents P < 0.05; The continuous data were expressed as x ± s, and the statistical differences were compared by ANOVA. The numerical data were expressed as n (%), and the chi-square test was used to compare the statistical differences. 3.3.2 Association between occupational exposure and biological aging trajectories in workers exposed to polycyclic aromatic hydrocarbons Table 4 shows the results of a multiple logistic regression model of the relationship between urinary OH-PAHs metabolites and accelerated aging trajectories with slow aging as the reference category. The results showed that the odds of being in the moderately accelerated aging increased by 39.2% for each increase of Ʃ-OHPAHs level in urine (OR = 1.392; 95%CI: 1.013 ~ 1.912), the odds of being in the highly accelerated aging trajectory increased by 61.2% (OR = 1.612; 95%CI: 1.093 ~ 2.376). For each additional natural logarithmic unit of 1-OHPyr level, the odds of being on a moderately accelerated aging trajectory increased by 47.2% (OR = 1.472; 95%CI: 1.058 ~ 2.048), the odds of being on a highly accelerated aging trajectory increased by 57.6% (OR = 1.576; 95%CI: 1.029 ~ 2.413). Similarly, for each additional natural logarithmic unit of 2-OHPhe levels, the odds of being on a highly accelerated aging trajectory increased by 46.7% (OR = 1.467; 95%CI: 1.075 ~ 2.001).Exposure to 2-OHFLU increasing by one natural logarithmic unit is associated with a lower likelihood of being in the moderately accelerated (OR = 0.602, 95% CI: 0.408–0.887) or highly accelerated trajectory(OR = 0.543, 95% CI: 0.334–0.882) . Table 4 Relationship between Urinary OH-PAHs Metabolites and Aging Trajectories of Occupational Workers Urine metabolites of OH-PAHs Moderate aging vs. slow aging High aging vs. slow aging OR (95% CI) P OR (95% CI) P Ʃ-OHPAHs a 1.392(1.013,1.912) 0.041 1.612(1.093,2.376) 0.016 2-OHNAP b 1.112(0.903,1.370) 0.316 1.011(0.801,1.276) 0.927 1-OHNAP b 0.901(0.672,1.209) 0.487 0.957(0.692,1.323) 0.790 3-OHFLU b 1.207(0.955,1.526) 0.115 1.211(0.926,1.583) 0.162 2-OHFLU b 0.602(0.408,0.887) 0.010 0.543(0.334,0.882) 0.014 2-OHPHE b 1.098(0.882,1.366) 0.405 1.467(1.075,2.001) 0.016 9-OHPHE b 1.102(0.743,1.634) 0.628 1.195(0.757,1.887) 0.445 1-OHPHE b 1.109(0.864,1.425) 0.417 0.856(0.620,1.180) 0.342 1-OHPYR b 1.472(1.058,2.048) 0.022 1.576(1.029,2.413) 0.036 3-OHChr b 0.733(0.536,1.003 ) 0.052 0.779(0.520,1.166) 0.225 6-OHChr b 1.139(0.894,1.451) 0.291 0.964(0.696,1.335) 0.825 9-OHBap b 1.060(0.859,1.308) 0.586 1.263(0.945,1.688) 0.114 Note: OR: odds ratio; CI: Confidence interval. Slow Aging: Slow Aging Trajectory, Moderate Aging: Moderately Accelerated Aging Trajectory, High Aging: Highly Accelerated Aging Trajectory. Model: aAdjust age, gender, education level, smoking status, alcohol consumption status, tea drinking status, exercise status, monthly income, night shift status. bAdjust for age, sex, education, smoking, drinking, tea, exercise, monthly income, night shift, and other urinary OH-PAHs metabolites. Bold indicates P < 0.05. The 11 ln-transformed OH-PAHs metabolites were categorized into three groups based on their respective P 33 and P 66 percentiles, with biological aging trajectory modeled as a categorical variable. The lowest tertile served as the exposure reference, and slow aging as the outcome reference. Multiple logistic regression analyses demonstrated that subjects in the highest tertile of Σ-OHPAHs and 1-OHPyr were respectively 83.4% (OR = 1.834, 95% CI: 1.024–3.283, P trend = 0.034) and 88.6% (OR = 1.886, 95% CI: 1.033–3.444, P trend = 0.037) more likely to be on a moderately accelerated aging trajectory compared to the lowest one-third. Those with the highest third of 3-OHChr had a 64.2% decreased risk of being on a moderately accelerated aging trajectory (OR = 0.358, 95% CI: 0.143–0.897), yet the trend test was insignificant. For highly accelerated aging trajectories, subjects in the highest one-third of Σ-OHPAHs, 2-OHPhe, and 1-OHPyr had respective odds of 2.002 (OR = 2.002, 95% CI: 1.008–3.978, P trend = 0.04), 3.374 (OR = 3.374, 95% CI: 1.133–10.052, P trend = 0.043), and 2.849 (OR = 2.849, 95% CI: 1.003–8.095, P trend = 0.043). These results are detailed in Table 5 . Table 5 Relationship between urinary OH-PAHs metabolites and aging trajectories of occupational workers Urine metabolites of OH-PAHs Moderate aging vs. slow aging High aging vs. slow aging OR (95% CI) P_trend OR (95% CI) P_trend Ʃ-OHPAHs a T1 1.000(Reference) 1.000(Reference) T2 1.047(0.615,1.783) 0.034 1.239(0.634,2.420) 0.043 T3 1.834(1.024,3.283) 2.002(1.008,3.978) 2-OHNAP b T1 1.000(Reference) 1.000(Reference) T2 0.570(0.299,1.087) 0.787 0.397(0.163,0.967) 0.439 T3 0.834(0.354,1.964) 0.744(0.244,2.263) 1-OHNAP b T1 1.000(Reference) 1.000(Reference) T2 1.815(0.977,3.371) 0.223 3.217(1.280,8.086) 0.301 T3 1.695(0.818,3.514) 1.887(0.698,5.102) 3-OHFLU b T1 1.000(Reference) 1.000(Reference) T2 1.100(0.551,2.195) 0.873 1.184(0.475,2.950) 0.976 T3 0.707(0.292,1.711) 0.988(0.301,3.243) 2-OHFLU b T1 1.000(Reference) 1.000(Reference) T2 0.782(0.414,1.478) 0.712 0.660(0.274,1.590) 0.761 T3 0.688(0.292,1.711) 0.367(0.124,1.088) 2-OHPHE b T1 1.000(Reference) 1.000(Reference) T2 0.983(0.521,1.857) 0.101 1.274(0.546,2.972) 0.034 T3 2.000(0.815,4.908) 3.374(1.133,10.052) 9-OHPHE b T1 1.000(Reference) 1.000(Reference) T2 1.478(0.772,2.832) 0.296 0.488(0.203,1.174) 0.830 T3 1.385(0.652,2.942) 0.912(0.342,2.433) 1-OHPHE b T1 1.000(Reference) 1.000(Reference) T2 0.818(0.414,1.615) 0.711 0.686(0.273,1.727) 0.075 T3 1.018(0.456,2.271) 0.611(0.218,1.717) 1-OHPYR b T1 1.000(Reference) 1.000(Reference) T2 0.973(0.567,1.668 ) 0.037 0.719(0.302,1.713) 0.019 T3 1.886(1.033,3.444 ) 2.849(1.003,8.095) 3-OHChr b T1 1.000(Reference) 1.000(Reference) T2 0.502(0.239,1.052 ) 0.289 0.935(0.360,2.428) 0.992 T3 0.358(0.143,0.897 ) 0.245(0.057,1.048) 6-OHChr b T1 1.000(Reference) 1.000(Reference) T2 1.633(0.819,3.256) 0.196 1.528(0.583,4.006) 0.289 T3 0.901(0.405,2.003) 0.617(0.202,1.882) 9-OHBap b T1 1.000(Reference) 1.000(Reference) T2 2.249(0.981,5.156) 0.393 2.798(0.961,8.146) 0.166 T3 3.23(1.259,8.283) 9.034(1.909,42.753) OR: odds ratio; CI: Confidence interval. Slow Aging: Slow Aging Trajectory, Moderate Aging: Moderately Accelerated Aging Trajectory, High Aging: Highly Accelerated Aging Trajectory. Model: aAdjust age, gender, education level, smoking status, alcohol consumption status, tea drinking status, exercise status, monthly income, night shift status. bAdjust for age, sex, education, smoking, drinking, tea, exercise, monthly income, night shift, and other urinary OH-PAHs metabolites. Bold indicates P < 0.05. 4. Discussion This study undertook the development of a biological age predictor for Chinese workers and assessed the correlation between PAH exposure and aging biomarkers.In a prospective cohort study, the research successfully applied group-based trajectory modeling (GBTM) techniques to identify three distinct aging trajectories within the study sample, thereby evaluating the rate of aging. Biological age, as a more precise measure of aging than chronological age, integrates various biomarkers related to physical function and provides comprehensive information about overall health. The results indicate that individuals exposed to high levels of PAHs tended to be biologically older than those exposed to low levels. Specifically, each natural logarithmic unit increase in Σ-OHPAHs raised the likelihood of moderate and high accelerated aging trajectories by 39.2% and 61.2%, respectively. PAHs, comprising hundreds of chemically related compounds with diverse structures and toxicities, including carcinogenicity and neurotoxicity, persistently pollute the environment. Exposure in occupational environments may involve multiple pathways, affecting the total absorbed dose (e.g.skin and inhalation exposure to contaminated air) 19 , 20 . People can be exposed to PAHs through direct inhalation, ingestion, or skin contact with air and surface soils containing these compounds 21 . Workers in coking plants are commonly exposed to PAHs. This study selected the content of PAH metabolites in urine as an indicator of human exposure, primarily based on its high specificity as a biomarker compared to blood samples, as well as the non-invasive collection advantages of urine samples.Given the complexity of PAHs in coking plants, 11 PAH hydroxy metabolites were used as a comprehensive exposure index. Human aging likely begins at or before reproductive maturity Identifying modifiable risk factors is crucial for maintaining health and quality of life during aging 22 . Chronological Age (CA) serves as a convenient method for assessing the aging status of individuals, but it cannot accurately reflect the differences in aging rates among individuals within the same CA group. The variation in cognitive function and health status increases with the growth of CA, indicating that CA may not accurately reveal the true aging differences between individuals.The proposed biological age (BA) describes aging relative to CA 23 . The estimation of biological age (BA) can be facilitated by omics indicators (such as telomere length) 24 , clinical indicators(BA measurement constructed based on the Klemera and Doubal method) 25 , and phenotypic indicators (such as frail phenotype, Frailty Index (FI), and functional aging index) 26 – 28 .This study employed a BA method constructed based on clinical indicators, which has the advantage of using chronological age (CA) as a standard biomarker in the model, significantly enhancing the accuracy of BA estimation, and elucidating the known "biomarker paradox" correlation. The study demonstrated that incorporating CA as a biomarker into the model significantly reduced the standard error of BA estimation. In predicting mortality and disease, the KDM method has been proven superior to other traditional methods 29 . In 2020, a longitudinal cohort study of Singaporean Chinese compared the differences in BA prediction of aging, disease, and mortality using MLR, PCA, and KDM methods 30 . The results showed that the KDM method's BA had significant predictive power in the younger group, and for groups with the same CA, the group with accelerated aging had a higher incidence of disease, worse health conditions, and more severe biological functional aging than the group with slower aging. This study applied the KDM method to construct BA in the population of Chinese occupational workers, and the results showed that KDM-BA highly matched with CA. In our study, the constructed KDM-BA included biochemical markers of liver function, kidney function, cardiovascular function, metabolic function, lipids, inflammation, and metabolism, which allows us to more comprehensively identify changes in the workers' bodies and distinguish earlier those in a preclinical state.Studies have shown that even at baseline without ischemic heart disease, stroke, or cancer, KDM-Accel to some extent distinguished individuals who may be in the subclinical stage of cardiovascular disease (CVD), indicating that KDM-Accel can reflect preclinical content earlier and better reflect early changes in the human body. Environmental factors, including air pollution, have a potential impact on the biological aging process. Long-term exposure to air pollution is considered one of the key factors leading to adverse age-related outcomes, particularly cardiovascular diseases (CVD) and respiratory system diseases 31 .Studies have shown that exposure to air pollution may even shorten the expected lifespan of individuals with good genetic endowment. Polycyclic aromatic hydrocarbons (PAHs) cause mitochondrial dysfunction, oxidative stress 32 , and DNA damage 33 , thereby leading to cellular aging and premature bodily aging 34 . Oxidative stress and the resulting chronic inflammation have been confirmed as significant pathogenic factors for the aging process and age-related diseases 17 .This study for the first time reveals the correlation between high exposure to PAHs and accelerated biological aging. Specifically, for each additional natural logarithmic unit of Ʃ-OHPAHs, the biological aging indicator of workers increases by 0.029 years; for each additional natural logarithmic unit of 1-OHPYR concentration, the biological aging indicator KDM-Accel increases by 0.028 years; for each additional natural logarithmic unit of 2-OHPHE concentration, the biological aging indicator KDM-Accel increases by 0.017 years. The results of this study are consistent with previous studies that workers occupationally exposed to PAHs exhibit shorter telomere lengths and accelerated lymphocyte DNA methylation age compared to those exposed to less PAHs 11 ; long-term skin exposure to PAHs may lead to higher skin aging scores 35 ; 1-OHPYR and 9-OHPHE are associated with methylation aging independently of other OH-PAHs and risk factors 36 . Furthermore, this study has several differences. First, we used objectively measured urinary OH-PAH metabolites to quantify the total exposure to PAHs from various sources, including diet, cooking, smoking, heating, transportation, waste burning, and occupational exposure. The use of OH-PAH metabolites not only improves the accuracy of exposure measurement but also allows us to distinguish the effects of different types of PAHs. Furthermore, among our study participants,we did not observe a significant correlation between smoking and biological aging; this observation may be partly due to the fact that smoking largely leads to exposure to naphthalene rather than phenanthrene and pyrene 37 . Second, compared to previous cellular aging indicators, biological age provides additional information—it has a similar scale to actual age and can be linked to the epigenetic behavior of certain age-related genes 38 . After multiple logistic analyses by gender grouping, the results showed that the male group was consistent with the total population(Table S8). However, in females, only 2-OHFLU showed consistent results, while Ʃ-OHPAHs were not statistically significant for the aging trajectory(Table S9). One possible reason is that estrogen has antioxidant and anti-inflammatory effects, and the biological aging caused by PAH exposure is through oxidative stress and chronic inflammation, which leads to a smaller impact of PAHs on the biological aging indicators of females 39 . Another important factor is that after stratification by gender, there were fewer female samples, and therefore, the impact of PAHs on the biological aging indicators of the female group could not be observed. Regarding the trajectory of aging, early studies identified different patterns of human aging trajectories by measuring indices such as frailty index 40 , health and functional items 41 , 42 , physical capacity, and educational items 43 , or other subjective indicators 44 . Compared with previous studies, our current research demonstrates the feasibility of long-term estimation of aging speed in the population of Chinese occupational workers. Compared to measuring aging at a single time point, the biological aging trajectory established based on aging biomarkers measured over a longer period may better capture the dynamics and heterogeneous nature of aging and is expected to accurately identify different populations with varying risks of age-related diseases or mortality. In the study, it was found that as the time of enrollment increased, the trajectory curve gradually rose. A possible explanation is that our population consists of workers under the age of 60, and most people experience a certain degree of chronic diseases around the age of 60 45 , with exposure to PAHs increasing the risk of such diseases. This will lead to an increasing trend in biological age over time for our study subjects. Previous studies constructed biological aging trajectories based on functional decline indicators, and the results showed that with the increase of time, the aging trajectory presented an upward trend, which is consistent with our study. We reconstructed the biological aging trajectory charts for coking plant workers and the control group of water treatment plant workers, and consistent results were found, both showing an increasing trend. Moreover, the aging trajectory chart constructed for coking plant workers showed a higher increase trend and a steeper slope than the aging trajectory of the control group water treatment plant, indicating that the biological aging trajectory may be caused by exposure to PAHs, resulting in a higher increase trend. Our results show that for every natural logarithmic unit increase in the urinary level of Σ-OHPAHs, the likelihood of being in a moderately accelerated aging trajectory increases by 39.2% (OR = 1.392; 95%CI: 1.013 ~ 1.912), and the likelihood of being in a highly accelerated aging trajectory increases by 61.2% (OR = 1.612; 95%CI: 1.093 ~ 2.376). For every natural logarithmic unit increase in 1-OHPyr, the likelihood of being in a moderately accelerated aging trajectory increases by 47.2% (OR = 1.472; 95%CI: 1.058 ~ 2.048), and the likelihood of being in a highly accelerated aging trajectory increases by 57.6% (OR = 1.576; 95%CI: 1.029 ~ 2.413). Additionally, for every natural logarithmic unit increase in 2-OHPHE, the likelihood of being in a highly accelerated aging trajectory increases by 46.7% (OR = 1.467; 95%CI: 1.075 ~ 2.001). We have further confirmed from the perspective of trajectories that PAHs can accelerate the process of biological aging. Although accelerated aging is a multi-system aging process with complex underlying biological mechanisms, our findings may provide an exciting new approach to studying how environmental pollution induces adverse health outcomes related to aging (including age-related diseases and mortality) through aging, offering more public health significance for the prevention of diseases in occupational workers. Our study has several advantages. Firstly, we conducted a prospective cohort study. Secondly, we conceptualized biological aging acceleration using multiple indicators, which are based on 12 biomarkers from routine examinations representing multiple organ systems. Thirdly, compared to a single time point assessment, we tracked aging acceleration at five time points using Group-Based Trajectory Modeling (GBTM), which may be more suitable for evaluating the aging process. The GBTM model explains the changes over time, differentiates the acceleration of aging over time, and is capable of identifying the heterogeneity of aging. Study limitations However, this study has certain limitations. Firstly, we only conducted a single measurement of polycyclic aromatic hydrocarbons (PAHs) in urine, but since urinary PAH metabolites can reflect long-term internal exposure indicators, PAHs have been used as internal exposure indicators in previous studies. Secondly, we tried to control all confounding factors, but due to the observational nature of the study, the possibility of residual confounding cannot be ruled out. Thirdly, because our correlation analysis was conducted in a cohort of occupational workers, biological aging was only measured in whole blood, so our correlation results may not be generalizable to other non-worker populations or to biological aging in other tissues. Fourthly, our cohort study had a relatively short duration, allowing us to observe only the short-term relationship between PAHs and biological aging trajectories. Lastly, our study population had a relatively small proportion of females. We plan to increase the number of female subjects in future studies to observe the impact of PAHs on biological aging in females. 5. Conclusion In summary, our study provides evidence that workers with higher exposure to polycyclic aromatic hydrocarbons (PAHs) experience accelerated aging compared to those with lower exposure. PAHs levels are positively associated with biological aging indicators and trajectories. Occupational PAHs exposure may predispose workers to accelerated aging. Reducing PAHs exposure could potentially reduce the aging rate among coke oven workers. Future longitudinal studies should employ larger samples with broader demographic representation to validate these findings and develop targeted interventions against occupationally accelerated aging. Declarations Corresponding author Jisheng Nie, MD, MPH, PhD. E-mail: [email protected] Data availability The data that support the findings of this study are not publicly available due to their containing information that could compromise the privacy of research participants. The data are available from the corresponding author upon reasonable request. Author contributions WYD and NJS conceptualized the research question. GSX, WWY and YLJ acquired the data.WYD and GSX performed all the analyses and visualized the results. WYD drafted the manuscript.GSX,ZHF,PBL and NJS helped with the interpretation and provided critical review. NJS, and NQ supervised all aspects of the project. All the authors read and approved the final manuscript. Acknowledgement We thank the participants and General Hospital of Taiyuan Iron & Steel (Group) Co., Ltd, Taiyuan, China, who assisted with the sample collection and Montreal Cognitive Assessment. Funding This study has been supported by the National Natural Science Foundation of China (No. 82073526 and No.81673143). Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Ethics approval and consent to participate This study was performed in accordance with the Declaration of Helsinki. The study protocol was approved by the Medical Ethics Committee of Shanxi Medical University (Approval No. 2020GLL037). Written informed consent was obtained from all individual participants included in the study. References Stec, A. A. et al. 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PLoS Med 17 , e1003138 (2020). https://doi.org:10.1371/journal.pmed.1003138 Additional Declarations No competing interests reported. Supplementary Files supplements.docx Cite Share Download PDF Status: Published Journal Publication published 31 Jan, 2026 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 14 Dec, 2025 Reviews received at journal 11 Dec, 2025 Reviews received at journal 03 Dec, 2025 Reviewers agreed at journal 27 Nov, 2025 Reviewers agreed at journal 16 Nov, 2025 Reviewers invited by journal 11 Nov, 2025 Editor assigned by journal 11 Nov, 2025 Editor invited by journal 02 Nov, 2025 Submission checks completed at journal 29 Oct, 2025 First submitted to journal 29 Oct, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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05:13:30","extension":"html","order_by":19,"title":"","display":"","copyAsset":false,"role":"acdc-reference","size":199773,"visible":true,"origin":"","legend":"","description":"","filename":"earlyproof.html","url":"https://assets-eu.researchsquare.com/files/rs-7945721/v1/b866e4d5f3566ea588ea3fde.html"},{"id":96434518,"identity":"bc4c9489-c26a-4bb4-8630-796360cfb4f5","added_by":"auto","created_at":"2025-11-21 05:13:29","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":91030,"visible":true,"origin":"","legend":"\u003cp\u003eFlow chart of the study participant selection process.\u003c/p\u003e","description":"","filename":"1.png","url":"https://assets-eu.researchsquare.com/files/rs-7945721/v1/a624fec615e2adf4b26e5cea.png"},{"id":96455098,"identity":"4b0cb542-cfc9-458b-a7ad-9cab46be80c6","added_by":"auto","created_at":"2025-11-21 10:03:33","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":337363,"visible":true,"origin":"","legend":"\u003cp\u003eBiological Aging Trajectory Chart and Heatmap of Correlation between PAHs metabolites in workers' urine\u003c/p\u003e\n\u003cp\u003e(1) Three trajectories have been identified among different populations: slow aging, moderate accelerated aging, and rapid accelerated aging. The different colored line segments represent different age trajectories.a.constructed for the total population, c .constructed for the population in the coking plant, d. constructed for the control group in the energy power plant.\u003c/p\u003e\n\u003cp\u003e(2) The values in the b figure are Spearman correlation coefficients. The greater the absolute value of the correlation coefficient, the deeper the color.\u003c/p\u003e","description":"","filename":"2.png","url":"https://assets-eu.researchsquare.com/files/rs-7945721/v1/518f222638214b8dba19509b.png"},{"id":96434509,"identity":"f526fa50-f819-43d4-9812-233da2c04b4a","added_by":"auto","created_at":"2025-11-21 05:13:29","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":227333,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003eRelationship between OH-PAHs metabolites in urine and biological aging indicator KDM-Accel in occupational workers\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eMode1：Adjust age, gender, education level, smoking status, alcohol consumption status, tea drinking status, exercise status, monthly income, night shift status.\u003c/p\u003e\n\u003cp\u003eMode2：Adjust age, gender, education level, smoking status, alcohol consumption status, tea drinking status, exercise status, monthly income, night shift status and other urinary OH-PAHs metabolites. Bold indicates P\u0026lt;0.05.\u003c/p\u003e","description":"","filename":"3.png","url":"https://assets-eu.researchsquare.com/files/rs-7945721/v1/ce63a51bd24093d4fe93ede0.png"},{"id":96434511,"identity":"a88b4645-759d-4895-accf-28c4fc433bde","added_by":"auto","created_at":"2025-11-21 05:13:29","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":239052,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003eDose-response relationship between urinary Ʃ-OHnap and biological aging indexes of workers in occupational workers.\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAdjusted covariates include age, gender, educational level, smoking status, alcohol consumption, physical exercise, frequency of night shifts, and other urinary OH-PAHs metabolites. The red line represents the dose-response relationship curve, with the dashed line indicating the 95% CI.\u003c/p\u003e","description":"","filename":"4.png","url":"https://assets-eu.researchsquare.com/files/rs-7945721/v1/d12d938c577013c63f8107df.png"},{"id":101690447,"identity":"1b42eeec-5a8c-4776-bcea-8f9ebf91f000","added_by":"auto","created_at":"2026-02-02 16:03:11","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":2827348,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7945721/v1/6f579e5b-effa-46ea-8b39-5ad094e83bee.pdf"},{"id":96455239,"identity":"0041fe13-6301-4449-80e0-53b34aac1938","added_by":"auto","created_at":"2025-11-21 10:03:49","extension":"docx","order_by":0,"title":"","display":"","copyAsset":false,"role":"supplement","size":373494,"visible":true,"origin":"","legend":"","description":"","filename":"supplements.docx","url":"https://assets-eu.researchsquare.com/files/rs-7945721/v1/da9b613e340b980e625899e5.docx"}],"financialInterests":"No competing interests reported.","formattedTitle":"Occupational polycyclic aromatic hydrocarbons (PAHs) exposure and aging trajectory : Epidemiologic evidence from a cohort of Chinese coke oven workers (2019-2023)","fulltext":[{"header":"1. Introduction","content":"\u003cp\u003ePolycyclic aromatic hydrocarbons (PAHs), a major component of air pollutants, originate from the incomplete combustion of organic matter. These compounds, composed of multiple fused benzene rings, form hydrocarbon substances with planar, angular, or cluster structures\u003csup\u003e\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e\u003c/sup\u003e..PAHs have been associated with a range of adverse health effects, including cardiovascular diseases\u003csup\u003e\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e\u003c/sup\u003e, cognitive dysfunction\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e\u003c/sup\u003e, cancer\u003csup\u003e\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e\u003c/sup\u003e, metabolic abnormalities\u003csup\u003e\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e\u003c/sup\u003e, and increased mortality\u003csup\u003e\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e\u003c/sup\u003e. Furthermore, the consequences related to PAHs exposure are closely linked to the aging process.\u003c/p\u003e\u003cp\u003eEpidemiological studies have indicated that PAHs exposure is correlated with various aging biomarkers. These include telomere shortening\u003csup\u003e\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e\u003c/sup\u003e, changes in DNA methylation patterns, increased mitochondrial DNA content\u003csup\u003e\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e\u003c/sup\u003e, and higher skin aging scores\u003csup\u003e\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e\u003c/sup\u003e. Such findings suggest that PAHs may play a role in accelerating both epigenetic and cellular aging processes\u003csup\u003e\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e,\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u003c/sup\u003e. However, it is important to note that the aforementioned aging-related biomarkers may have certain limitations.\u003c/p\u003e\u003cp\u003eIn recent years, the quantification of biological aging in conjunction with standard clinical parameters has been proven to be one of the most accurate methods for predicting diseases and mortality\u003csup\u003e\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e,\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e\u003c/sup\u003e. This approach is also becoming a key method for understanding how environmental exposures and interventions influence the risk of chronic diseases\u003csup\u003e\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e\u003c/sup\u003e. Unlike mere chronological aging, biological aging reflects the cumulative cellular and molecular damage that leads to functional decline\u003csup\u003e\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e\u003c/sup\u003e.\u003c/p\u003e\u003cp\u003eRecent analysis of the National Health and Nutrition Examination Survey (NHANES) database has revealed a correlation between exposure to mixed PAHs and increased aging\u003csup\u003e\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e\u003c/sup\u003e. Among these, 2-naphthol has been identified as a key component of PAHs associated with aging. Nonetheless, the precise relationship between PAHs exposure and biological age trajectories remains unclear. Specifically, it is not well understood how PAHs affect aging-related pathways in the early stages of exposure or to what extent they influence the aging process.\u003c/p\u003e\u003cp\u003eAdditionally, when compared to the general population residing outside industrial areas, coal miners and coking plant workers are exposed to PAHs for longer durations and at higher intensities within more complex environmental mixtures. This chronic exposure may not only increase the risk of age-related diseases but also potentially accelerate biological aging itself, leading to the earlier onset of aging-related functional decline\u003csup\u003e\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e,\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e,\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e\u003c/sup\u003e.\u003c/p\u003e\u003cp\u003eTherefore, this study was designed as a prospective cohort study with the aim of assessing the impact of PAHs on biological age and aging trajectories. By developing a biological aging prediction model based on newly established biomarkers of biological aging and constructing aging trajectories, this study seeks to provide valuable insights into the long-term effects of PAHs exposure. The results of this research will offer scientific evidence for early disease intervention and risk stratification through the assessment of biological aging trajectories. Moreover, the findings will serve as an epidemiological basis to guide occupational workers in implementing effective protective measures to mitigate the health risks associated with PAHs exposure.\u003c/p\u003e"},{"header":"2. Material and methods","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e\u003ch2\u003e2.1 Study participants\u003c/h2\u003e\u003cp\u003eThis study used cluster sampling to build a professional worker cohort, recruiting 610 coke oven workers and 454 from a water treatment plant in Shanxi Province in 2019. Follow-ups were done in 2020\u0026ndash;2023. Participants were informed of the study\u0026rsquo;s purpose and procedures, advised to maintain a light diet, and required to fast for at least 12 hours before sample collection. At enrollment, each participant completed a questionnaire on demographics, lifestyle, and medical history, conducted face-to-face by trained investigators using a standardized \"Health Survey Form\". For aging trajectory analysis, 763 coking plant cohort participants aged\u0026thinsp;\u0026ge;\u0026thinsp;20 with baseline measurements in 2019 and four aging assessments (KDM-BA) were included.After excluding those with missing PAH exposure or demographic data, 673 subjects remained for longitudinal analysis to explor\u003cspan type=\"Underline\" class=\"Underline\" name=\"Emphasis\"\u003ee\u003c/span\u003e the association between PAHs exposure and aging trajectories. The flowchart is shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003ea.We screened 55,720 occupational examinees in Shanxi, 2019. Excluding non-frontline workers and those aged\u0026thinsp;\u0026lt;\u0026thinsp;20 or \u0026gt;\u0026thinsp;80 years or with missing biomarkers yielded 27,806 participants (Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003eb). The occupational cohort served as the training set and the coke oven cohort as the test set for a biological aging prediction model.This study was approved by the Medical Ethics Committee of the Shanxi Medical University and all participants signed informed consent form.The serial number is 2020GLL037.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec4\" class=\"Section2\"\u003e\u003ch2\u003e2.2 Baseline data collection\u003c/h2\u003e\u003cdiv id=\"Sec5\" class=\"Section3\"\u003e\u003ch2\u003e2.2.1 General questionnaire collection\u003c/h2\u003e\u003cp\u003eThe pre-enrollment questionnaire primarily covered seven sections: worker demographics (age, gender, ethnicity, height, weight, marital status, etc.), residential info, dietary habits (vegetables, fruits, meats, etc.), lifestyle habits (smoking, drinking, etc.), occupational history, medical history, and sleep patterns. Smokers are defined as those smoking\u0026thinsp;\u0026ge;\u0026thinsp;1 cigarette/day for \u0026ge;\u0026thinsp;6 months; drinkers as \u0026ge;\u0026thinsp;3 times/week for \u0026ge;\u0026thinsp;1 year. Marital status includes single, married, etc. BMI is calculated as weight/height\u0026sup2; (kg/m\u0026sup2;). Each survey took\u0026thinsp;\u0026le;\u0026thinsp;15 minutes to ensure quality.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec6\" class=\"Section3\"\u003e\u003ch2\u003e2.2.2 Routine physical examination\u003c/h2\u003e\u003cp\u003eAll participants underwent a health examination, with blood samples and morning urine collected. Testing was done within 24 hours, including biochemical tests, blood pressure, ECG, and ultrasound.\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv id=\"Sec7\" class=\"Section2\"\u003e\u003ch2\u003e2.3 Determination of urinary PAHs metabolites\u003c/h2\u003e\u003cp\u003eDuring physical exams, professionals collected 50 mL urine samples using sterile cups. Samples were promptly transported to the lab, measured for specific gravity, and sub-packed into 5-mL cryotubes, then frozen at -80\u0026deg;C. High-performance liquid chromatography-mass spectrometry (HPLC-MS) measured 11 PAH metabolites, including 2-OHNAP, 10-OHNAP, etc. Detailed procedures and quality control data are in previous studies(Table \u003cspan refid=\"MOESM1\" class=\"InternalRef\"\u003eS1\u003c/span\u003e).\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec8\" class=\"Section2\"\u003e\u003ch2\u003e2.4 Selection of biomarkers\u003c/h2\u003e\u003cp\u003eBiomarker selection considered their role in aging, prior aging study applications, and availability in the occupational worker dataset, along with their Pearson correlation with chronological age. The 38 biomarkers analyzed fall into seven categories: metabolic function (BMI, blood sugar, etc.), cardiac function (blood pressure, heart rate), pulmonary function (FVC, FEV1, FEV1/FVC), renal function (urine pH, UA, etc.), liver function (ALT, AST), immune function (lymphocyte count, etc.), and blood cell counts (white blood cell count, etc.). Pearson correlation assessed the relationship between these biomarkers and age (Table S2).\u003c/p\u003e\u003cp\u003eNotably, platelet volume and count, hemoglobin, red blood cell count, hematocrit, systolic and diastolic blood pressure demonstrated high intercorrelation (r\u0026thinsp;\u0026gt;\u0026thinsp;0.7). Following Klemera and Doubal's dimensionality reduction approach, a single clinically relevant biomarker was systematically selected from each correlated parameter group as a representative indicator (e.g., platelet count for platelets, red blood cell count for erythrocytes, systolic blood pressure for blood pressure).Ultimately, 12 biomarkers were selected for biological age calculation, all with correlation coefficients\u0026thinsp;\u0026gt;\u0026thinsp;0.1. These included ALT, RBC, HDL, TC, MCV, MCHC, RDW, PLT, LYC, BUR, and SBP.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec9\" class=\"Section2\"\u003e\u003ch2\u003e2.5 Statistical analysis\u003c/h2\u003e\u003cp\u003eData from questionnaires and standardized instruments were entered into a database using EpiData 3.1 software, with subsequent data cleaning performed in SPSS 25. Descriptive statistics were used to summarize the baseline characteristics of the study population during their initial physical examination. Socio-demographic characteristics were presented as mean\u0026thinsp;\u0026plusmn;\u0026thinsp;standard deviation (X\u0026thinsp;\u0026plusmn;\u0026thinsp;S) or frequency (percentage) (N%). Levels of polycyclic aromatic hydrocarbon hydroxy metabolites in urine were transformed using natural logarithm (Ln transformation) to achieve approximate normality for continuous variables, or categorized into three levels (T1, T2, T3) based on tertiles for categorical variables. Categorical variables were compared using chi-square tests, while continuous variables were analyzed with t-tests to assess differences in characteristics among different aging trajectory groups. Spearman correlation analysis was applied to evaluate the correlations among the 11 polycyclic aromatic hydrocarbons.\u003c/p\u003e\u003cp\u003eBiological age (KDM-BA) and biological aging acceleration (KDM-Accel) were precisely measured according to the method proposed by Klemera and Doubal. The prediction accuracy was assessed by calculating the Pearson correlation coefficient (R) and root mean square error (RMSE) between chronological age and KDM-BA. Moreover, to account for the effect of chronological age (CA), KDM-BA acceleration (KDM-Accel) was defined as the residual from the linear regression of KDM-BA on CA. The training dataset demonstrated a near-perfect Spearman's rank correlation (ρ\u0026thinsp;=\u0026thinsp;0.99) between chronological age and biological age in the occupational worker cohort, with RMSE\u0026thinsp;=\u0026thinsp;0.205 years and MAE\u0026thinsp;=\u0026thinsp;0.165 years (Fig. S2a). In the testing dataset focusing on the coking plant cohort, the correlation coefficient R2 was 0.99, with RMSE of 0.234 and MAE of 0.175,as seen in supplemental Fig.\u0026nbsp;2b. Both indicated good model predictability, verifying the effectiveness of the predictor. KDM-Accel is planned to be included as a biological aging indicator in subsequent analyses.\u003c/p\u003e\u003cp\u003eTo explore the correlations between different hydroxy polycyclic aromatic hydrocarbon (OH-PAH) metabolites and the aging process, a multiple linear regression model was constructed, with the aging acceleration indicator (KDM-Accel) as the response variable and various OH-PAH metabolites (as continuous variables) as explanatory variables. The model adjusted for covariates including age, gender, education level, smoking status, alcohol consumption, tea consumption, exercise status, monthly income, shift work status, and other polycyclic aromatic hydrocarbon metabolites in urine. For missing covariate data, a dummy variable encoding method was used for missing categorical variables, and the median of continuous variables was used as a substitute for estimation. Additionally, restricted cubic spline models (RCS) were applied to analyze the dose-response relationship between polycyclic aromatic hydrocarbon exposure and the biological aging rate of workers.\u003c/p\u003e\u003cp\u003eKDM-Accel was calculated at five specific time points. Group-based trajectory modeling (GBTM) based on KDM-Accel was used to identify different aging trajectories at these five time points. The specific study design flow chart is shown in Fig.S2. The GBTM method is based on the assumption that the study population consists of different groups defined by individual developmental trajectories, and this process was implemented in SAS PROC TRAJ, with aging trajectories determined empirically. The number of subgroups of the biological aging index and the functional model for each group were determined by the following principles: (1) Bayesian Information Criterion (BIC), (2) average posterior probability of members (each biological aging trajectory class\u0026thinsp;\u0026ge;\u0026thinsp;0.7), and (3) reasonable distribution of study subjects in trajectory classes. A series of possible parameters for the GBTM model were considered, including fitting cubic, quadratic, and linear terms, with variations in one to six trajectory groups. The best-fitting trajectory model (3Group) was ultimately selected. These different aging trajectories consist of individuals with similar aging acceleration patterns, demonstrating the dynamic and heterogeneous process of aging. Specific parameter selection details are shown in Tables S3-S6 and Fig.S3.\u003c/p\u003e\u003cp\u003eThis study further employs multiple logistic regression analysis to assess the association between the tertiles of exposure levels to polycyclic aromatic hydrocarbons (PAHs) and aging trajectories .The lowest tertile was used as the reference exposure level, and the slow-aging trajectory as the reference outcome category. Odds ratios (ORs) and their 95% confidence intervals (CIs) were calculated. Trend tests were performed by assigning the median of each tertile to the index and including it as a continuous variable in the model. Additionally, these indicators were included in the analysis as continuous variables (per 10-unit increase). To explore the impact of gender differences on the relationship between polycyclic aromatic hydrocarbons and aging trajectories, stratified analysis by gender was conducted. Multinomial logistic regression models were used to estimate ORs and 95% CIs, and further explored the relationships between the 11 polycyclic aromatic hydrocarbons and aging trajectories.\u003c/p\u003e\u003cp\u003eAll analyses were conducted using SAS 9.7 (SAS Institute, Cary, NC), and figures were drawn using R 4.3.2. All p-values were two-sided. A p-value\u0026thinsp;\u0026lt;\u0026thinsp;0.05 was considered statistically significant.The technology roadmap of this study is shown in Fig.S4.\u003c/p\u003e\u003c/div\u003e"},{"header":"3. Results","content":"\u003cdiv id=\"Sec11\" class=\"Section2\"\u003e\n \u003ch2\u003e3.1 Basic characteristics\u003c/h2\u003e\n \u003cp\u003eAmong the 673 participants in the longitudinal study, the average age was 44.71\u0026thinsp;\u0026plusmn;\u0026thinsp;7.32 years, and 89.45% were male(Table S7). Over half of the workers had an educational level above high school. Approximately 39.23% and 59.14% of the study subjects never smoked or drank alcohol, respectively, and 426 individuals (63.3% of the total) consumed tea. However, most subjects did not engage in healthy physical activities.\u003c/p\u003e\n \u003cp\u003eCompared with the control group, coking plant workers were younger and had a higher proportion of males (91.91% vs. 86.21%, P\u0026thinsp;=\u0026thinsp;0.017), and a smaller proportion of those who often participated in physical exercise (32.64% vs. 41.03%, P\u0026thinsp;\u0026lt;\u0026thinsp;0.001). No significant differences were found between the two groups in terms of educational level, marital status, smoking status, drinking status, tea drinking status, and night shift status (P\u0026thinsp;\u0026gt;\u0026thinsp;0.05). Although there was no significant difference in KDM-Accel between the two groups, the average value was higher in the coking plant group than in the water treatment plant group.\u003c/p\u003e\n \u003cp\u003eThe distribution of OH-PAHs in the urine of workers have been adjusted for urine specific gravity(Table S7). The detection rates of the 11 OH-PAHs metabolites were all above 75%. The geometric mean of OH-PAHs metabolites was calculated. The highest concentration of OH-PAHs metabolites was 2-OHNap (0.94 \u0026micro;g/L), and the lowest was 9-OHBap (0.07 \u0026micro;g/L). In the control group, the concentrations of OH-PAHs metabolites in the urine of water treatment plant workers were 9-OHPhe (0.37 \u0026micro;g/L) and 3-OHChr (0.04 \u0026micro;g/L). The levels of OH-PAHs in the urine of coking plant workers were consistently higher than those in the control group from the water treatment plant.\u003c/p\u003e\n \u003cp\u003eGBTM identified three potential categories of biological aging trajectories (Fig. \u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003ea).Approximately 14.86% of the study subjects were classified into the slow aging trajectory group, all with negative KDM-Accel values, indicating that their biological age was generally younger than their chronological age across the five measures. About 59.14% were placed in the moderately accelerated aging trajectory group, showing moderate levels of accelerated aging initially. Individuals in this group had significantly higher urinary PAHs metabolite concentrations at baseline than those in the slow aging trajectory group. The remaining 26% were in the highly accelerated aging group, showing a high rate of accelerated aging across all four measures. The age of this group was 45.41\u0026thinsp;\u0026plusmn;\u0026thinsp;7.12 years, and the concentration of PAHs metabolites in urine was the highest(Table \u003cspan class=\"InternalRef\"\u003e1\u003c/span\u003e). Figure \u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003eb displays the pairwise Spearman\u0026apos;s rank correlations (\u003cem\u003e\u0026rho;\u003c/em\u003e) among 11 polycyclic aromatic hydrocarbons (PAHs), with values ranging from 0.04 to 0.75. Specifically, 2-OHNap was moderately correlated with 2-OHPhe and 1-OHNap (r\u0026thinsp;=\u0026thinsp;0.52, 0.57), and strongly correlated with 3-OHChr and 9-OHBap (r\u0026thinsp;=\u0026thinsp;0.75). Sensitivity analysis was subsequently conducted, and aging trajectories were constructed separately for the coking plants and power plants using the GBTM method. The results showed that the trajectories of the two plants were homogeneous and showed increasing trends, with coking plants exhibiting a faster growth rate, a larger slope, and a higher KDM-Accel value than the control group (Fig. \u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003ec and \u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003ed).\u003c/p\u003e\n \u003cdiv align=\"left\" class=\"colspec\"\u003e\u003cbr\u003e\u003c/div\u003e\n \u003ctable id=\"Tab1\" border=\"1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eBasic Characteristics of coke oven workers (n\u0026thinsp;=\u0026thinsp;673)\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\" rowspan=\"3\"\u003e\n \u003cp\u003evariable\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\" rowspan=\"3\"\u003e\n \u003cp\u003eTotal population\u003c/p\u003e\n \u003cp\u003en\u0026thinsp;=\u0026thinsp;673\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eAging trajectories\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003cth align=\"left\" rowspan=\"3\"\u003e\n \u003cp\u003e\u003cem\u003eP\u003c/em\u003e value\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eSlow aging group\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eModerately accelerated aging group\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eHighly accelerated aging group\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003en\u0026thinsp;=\u0026thinsp;100\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003en\u0026thinsp;=\u0026thinsp;398\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003en\u0026thinsp;=\u0026thinsp;175\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAge (years)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e44.71\u0026thinsp;\u0026plusmn;\u0026thinsp;7.32\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e44.61\u0026thinsp;\u0026plusmn;\u0026thinsp;6.77\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e44.43\u0026thinsp;\u0026plusmn;\u0026thinsp;7.53\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e45.41\u0026thinsp;\u0026plusmn;\u0026thinsp;7.12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.495\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003egender\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026lt;\u0026thinsp;0.001\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eman\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e602(89.45)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e77(77.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e357(89.70)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e168(96.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003ewoman\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e71(10.55)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e23(23.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e41(10.30)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e7(4.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eEducational attainment (years, N%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026lt;\u0026thinsp;0.001\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1\u0026ndash;9\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e176(26.15)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e28(28.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e100(25.13)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e48(27.43)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e10\u0026ndash;12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e421(62.56)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e61(61.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e247(62.06)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e113(64.57)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u0026ge;\u0026thinsp;13\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e76(11.29)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e11(11.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e51(12.81)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e14(8.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003emarital status\u003c/p\u003e\n \u003cp\u003e(Yes, N%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.197\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eYes\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e636(94.50)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e93(93.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e373(93.72)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e170(97.14)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNo\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e37(5.50)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e7(7.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e25(6.28)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e5(2.86)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eSmoking\u003c/p\u003e\n \u003cp\u003e(Yes, N%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e409(60.77)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e56(56.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e254(63.82)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e99(56.57)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.150\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAlcohol consumption\u003c/p\u003e\n \u003cp\u003e(Yes, n%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e275(40.86)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e47(47.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e156(39.20)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e72(41.14)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.364\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eTea drinking condition\u003c/p\u003e\n \u003cp\u003e(Yes, n%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e426(63.30)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e59(59.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e254(63.82)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e113(64.57)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.618\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eExercise status\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.676\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003enever\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e186(27.64)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e23(23.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e115(28.89)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e48(27.43)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eOccasionally\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e243(36.11)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e38(38.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e137(34.42)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e68(38.86)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eoften\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e244(36.26)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e39(39.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e146(36.68)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e59(33.71)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNight shift (yes, n%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e467(69.60)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e70(70.00)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e282(71.21)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e115(65.71)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.418\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eKDM (years)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e44.61\u0026thinsp;\u0026plusmn;\u0026thinsp;7.33\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e44.28\u0026thinsp;\u0026plusmn;\u0026thinsp;6.77\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e44.30\u0026thinsp;\u0026plusmn;\u0026thinsp;7.55\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e45.48\u0026thinsp;\u0026plusmn;\u0026thinsp;7.12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.127\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003ekdm_advance (years old)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e-0.11\u0026thinsp;\u0026plusmn;\u0026thinsp;0.17\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e-0.33\u0026thinsp;\u0026plusmn;\u0026thinsp;0.12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e-0.13\u0026thinsp;\u0026plusmn;\u0026thinsp;0.12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.07\u0026thinsp;\u0026plusmn;\u0026thinsp;0.11\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026lt;\u0026thinsp;0.001\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eUrine PAHs metabolites (\u0026micro;g/L)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2-OHNap\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.68\u0026thinsp;\u0026plusmn;\u0026thinsp;1.22\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.49\u0026thinsp;\u0026plusmn;\u0026thinsp;0.77\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.67\u0026thinsp;\u0026plusmn;\u0026thinsp;1.03\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.84\u0026thinsp;\u0026plusmn;\u0026thinsp;1.71\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.544\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1-OHNap\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.25\u0026thinsp;\u0026plusmn;\u0026thinsp;1.16\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.17\u0026thinsp;\u0026plusmn;\u0026thinsp;0.35\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.27\u0026thinsp;\u0026plusmn;\u0026thinsp;1.39\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.27\u0026thinsp;\u0026plusmn;\u0026thinsp;0.85\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.723\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3-OHFlu\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.09\u0026thinsp;\u0026plusmn;\u0026thinsp;0.15\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.07\u0026thinsp;\u0026plusmn;\u0026thinsp;0.08\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.09\u0026thinsp;\u0026plusmn;\u0026thinsp;0.13\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.11\u0026thinsp;\u0026plusmn;\u0026thinsp;0.19\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.866\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2 OHFlu\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.15\u0026thinsp;\u0026plusmn;\u0026thinsp;0.18\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.13\u0026thinsp;\u0026plusmn;\u0026thinsp;0.12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.15\u0026thinsp;\u0026plusmn;\u0026thinsp;0.16\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.16\u0026thinsp;\u0026plusmn;\u0026thinsp;0.24\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.408\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2-OHPhe\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.28\u0026thinsp;\u0026plusmn;\u0026thinsp;0.47\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.19\u0026thinsp;\u0026plusmn;\u0026thinsp;0.22\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.25\u0026thinsp;\u0026plusmn;\u0026thinsp;0.35\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.39\u0026thinsp;\u0026plusmn;\u0026thinsp;0.74\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.046\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e9-OHPhe\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.42\u0026thinsp;\u0026plusmn;\u0026thinsp;0.55\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.35\u0026thinsp;\u0026plusmn;\u0026thinsp;0.29\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.43\u0026thinsp;\u0026plusmn;\u0026thinsp;0.50\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.45\u0026thinsp;\u0026plusmn;\u0026thinsp;0.75\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.151\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1-OHPhe\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.10\u0026thinsp;\u0026plusmn;\u0026thinsp;0.16\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.09\u0026thinsp;\u0026plusmn;\u0026thinsp;0.12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.10\u0026thinsp;\u0026plusmn;\u0026thinsp;0.13\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.11\u0026thinsp;\u0026plusmn;\u0026thinsp;0.24\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.345\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1-OHPyr\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.20\u0026thinsp;\u0026plusmn;\u0026thinsp;0.25\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.14\u0026thinsp;\u0026plusmn;\u0026thinsp;0.15\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.21\u0026thinsp;\u0026plusmn;\u0026thinsp;0.26\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.23\u0026thinsp;\u0026plusmn;\u0026thinsp;0.29\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.049\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3-OHChr\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.06\u0026thinsp;\u0026plusmn;\u0026thinsp;0.09\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.04\u0026thinsp;\u0026plusmn;\u0026thinsp;0.04\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.05\u0026thinsp;\u0026plusmn;\u0026thinsp;0.07\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.07\u0026thinsp;\u0026plusmn;\u0026thinsp;0.13\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.627\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e6-OHChr\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.08\u0026thinsp;\u0026plusmn;\u0026thinsp;0.08\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.07\u0026thinsp;\u0026plusmn;\u0026thinsp;0.05\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.09\u0026thinsp;\u0026plusmn;\u0026thinsp;0.07\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.09\u0026thinsp;\u0026plusmn;\u0026thinsp;0.10\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.272\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e9-OHBap\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.06\u0026thinsp;\u0026plusmn;\u0026thinsp;0.09\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.05\u0026thinsp;\u0026plusmn;\u0026thinsp;0.05\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.06\u0026thinsp;\u0026plusmn;\u0026thinsp;0.07\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.07\u0026thinsp;\u0026plusmn;\u0026thinsp;0.12\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.473\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eƩ-OH PAHs\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2.19\u0026thinsp;\u0026plusmn;\u0026thinsp;2.67\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.65\u0026thinsp;\u0026plusmn;\u0026thinsp;1.25\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2.17\u0026thinsp;\u0026plusmn;\u0026thinsp;2.46\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2.57\u0026thinsp;\u0026plusmn;\u0026thinsp;3.52\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.247\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\" colspan=\"6\"\u003e\n \u003cp\u003e\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.05;\u003c/p\u003e\n \u003cp\u003eThe continuous data were expressed as x\u0026thinsp;\u0026plusmn;\u0026thinsp;s, and the statistical differences were compared by ANOVA.\u003c/p\u003e\n \u003cp\u003eThe numerical data were expressed as n (%), and the chi-square test was used to compare the statistical differences.\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec12\" class=\"Section2\"\u003e\n \u003ch2\u003e3.2 Association between occupational exposure and biological aging of workers exposed to polycyclic aromatic hydrocarbons\u003c/h2\u003e\n \u003cp\u003eThis study first explored the linear correlation between PAHs and the biological aging indicator (KDM-Accel). Using the natural logarithm-transformed PAH metabolites as continuous variables, we performed multiple linear regression analyses.Table\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003e shows the results of the multiple linear regression model after controlling for covariables. The analysis indicates that exposure to 1-OHPyr and 2-OHPHE significantly increases workers\u0026apos; biological age .Conversely, 2-OHFlu shows a negative correlation with biological age. Specifically, a one-unit natural log increase in 1-OHPYR concentration is associated with a 0.028-year increase in KDM-Accel; a similar increase in 2-OHPHE concentration corresponds to a 0.017-year rise. Conversely, a one-unit natural log increase in 2-OHNAP concentration is linked to a 0.026-year decrease in KDM-Accel. After further covariate adjustment, these effects weakened but remained statistically significant (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05). For total PAH metabolites, Model 1 adjusted for age, sex, and education. Results show that a one-unit natural log increase in \u0026Sigma;-OHPAHs is associated with a 0.029-year increase in KDM-Accel. In Model 2, further adjustment for smoking, drinking, tea consumption, exercise, monthly income, and night shift status shows a 0.032-year increase in KDM-Accel per one-unit natural log increase in \u0026Sigma;-OHPAHs.\u003c/p\u003e\n \u003cdiv class=\"gridtable\"\u003e\n \u003cdiv align=\"left\" class=\"colspec\"\u003e\u003cbr\u003e\u003c/div\u003e\n \u003ctable id=\"Tab2\" border=\"1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eRelationship between urinary OH-PAHs metabolites and occupational workers\u0026apos; biological aging index KDM-Accel\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\" rowspan=\"2\"\u003e\n \u003cp\u003eIn urine\u003c/p\u003e\n \u003cp\u003eOH-PAHs\u003c/p\u003e\n \u003cp\u003eMetabolites\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eMultiple linear regression results \u0026beta; (95% CI)\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eModel one\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eP-value\u003c/em\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eModel two\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eP-value\u003c/em\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eƩ-OHPAHs \u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.029(0.013,0.046)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026lt;\u0026thinsp;0.001\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.032(0.015,0.049)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026lt;\u0026thinsp;0.001\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2-OHNAP \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.003(-0.007,0.013)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.518\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.005(-0.005,0.015)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.347\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1-OHNAP \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.001(-0.013,0.015)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.862\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.001(-0.013,0.015)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.860\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3-OHFLU \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.007(-0.005,0.018)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.247\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.007(-0.005,0.018)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.264\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2-OHFLU \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e-0.026(-0.040,-0.011)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026lt;\u0026thinsp;0.001\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e-0.022(-0.037,-0.007)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.004\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2-OHPHE \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.017(0.005,0.028)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.005\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.014(0.002,0.026)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.019\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e9-OHPHE \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.003(-0.0169,0.022)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.736\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.005(-0.015,0.025)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.608\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1-OHPHE \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e-0.001(-0.014,0.011)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.836\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e-0.003(-0.015,0.009)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.615\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1-OHPYR \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.028(0.011,0.045)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.001\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.028(0.011,0.045)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.001\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3-OHChr \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e-0.012(-0.029,0.005)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.175\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e-0.011(-0.028,0.006)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.209\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e6-OHChr \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e-0.001(-0.014,0.013)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.972\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e-0.001(-0.014,0.013)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.900\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e9-OHBap \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.013(0.002,0.024)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.020\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.012(0.001,0.023)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.042\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n \u003c/div\u003e\n \u003cp\u003eModel 1:adjusted for age, sex, and education level. b.adjusted for age, sex, education level, and other urinary OH-PAHs metabolites.\u003c/p\u003e\n \u003cp\u003eModel 2: adjusted for age, gender, education level, smoking status, drinking status, tea consumption status, exercise status, monthly income, and night shift status. b adjusted for age, gender, education level, smoking status, drinking status, tea consumption status, exercise status, monthly income, night shift status, and other urinary OH-PAHs metabolites. Bold indicates P\u0026thinsp;\u0026lt;\u0026thinsp;0.05.\u003c/p\u003e\n \u003cp\u003eLog-transformed concentrations of 11 individual PAH metabolites and \u0026Sigma;-OHPAHs were partitioned into tertiles based on their 33rd (P33) and 66th (P66) percentiles, defining three exposure strata: Group 1, Group 2,and Group 3.Categorical variables were included in the model for analysis. After adjusting for covariates, a generalized linear regression model was used to analyze the relationship between hydroxy-PAHs levels and KDM-Accel, as shown in Fig. \u003cspan class=\"InternalRef\"\u003e3\u003c/span\u003e. The model was adjusted for age, sex, education, smoking status, alcohol consumption, tea consumption, exercise status, monthly income, night shift status, and other urinary PAH metabolites. The analysis reveals that compared to Group 1, Group 3 of \u0026Sigma;-OHPAHs is associated with a 0.033-year increase in the workers\u0026apos; biological aging indicator. Trend testing indicates a significant upward trend in the biological aging indicator with increasing urinary \u0026Sigma;-OHPAHs levels (P_trend\u0026thinsp;=\u0026thinsp;0.034). For 1-OHPyr, Group 3 versus Group 1 is associated with a 0.063-year increase in the biological aging indicator (OR\u0026thinsp;=\u0026thinsp;0.063; 95%CI: 0.0215\u0026thinsp;~\u0026thinsp;0.1048). Trend testing also shows a significant upward trend in the biological aging indicator with increasing urinary 1-OHPyr levels (P_trend\u0026thinsp;=\u0026thinsp;0.006).\u003c/p\u003e\n \u003cp\u003eThis study used restricted cubic splines to analyze the dose-response relationship between workers\u0026apos; \u0026Sigma;-OHPAHs and the KDM-Accel. Using the median concentration of \u0026Sigma;-OHPAHs as the reference point, three knots (P\u003csub\u003e10\u003c/sub\u003e, P\u003csub\u003e50\u003c/sub\u003e, and P\u003csub\u003e90\u003c/sub\u003e) were selected for analysis, with results shown in Fig. \u003cspan class=\"InternalRef\"\u003e4\u003c/span\u003ea. A significant dose-response relationship was found between \u0026Sigma;-OHPAHs and the KDM-Accel (P\u003csub\u003eoverall\u003c/sub\u003e \u0026lt; 0.001). As urinary \u0026Sigma;-OHPAHs concentrations increase, workers\u0026apos; biological aging rates also increase in a linear dose-response manner. Additionally, using the median concentration of 1-OHPYR as the reference point and selecting three knots (P\u003csub\u003e10\u003c/sub\u003e, P\u003csub\u003e50\u003c/sub\u003e, and P\u003csub\u003e90\u003c/sub\u003e) for analysis, the results (Fig. \u003cspan class=\"InternalRef\"\u003e4\u003c/span\u003eb)indicate a significant dose-response relationship between 1-OHPYR levels and the KDM-Accel (P\u003csub\u003eoverall\u003c/sub\u003e \u0026lt; 0.001). As urinary 1-OHPYR concentrations increase, workers\u0026apos; biological aging rates also increase, exhibiting a non-linear dose-response relationship.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec13\" class=\"Section2\"\u003e\n \u003ch2\u003e3.3 Association between occupational exposure and biological aging trajectories in workers exposed to polycyclic aromatic hydrocarbons\u003c/h2\u003e\n \u003cdiv id=\"Sec14\" class=\"Section3\"\u003e\n \u003ch2\u003e3.3.1 Basic information about workers\u003c/h2\u003e\n \u003cp\u003eThe longitudinal data included 1064 workers who participated in the baseline survey and 673 workers who completed the follow-up. Table \u003cspan class=\"InternalRef\"\u003e3\u003c/span\u003e compares the baseline information of 1064 workers with 673 workers included in the cohort study. The results showed that there were no statistically significant differences in age, sex, marital status, smoking status, drinking status, tea drinking status and night shift status between the two groups.\u003c/p\u003e\n \u003cdiv class=\"gridtable\"\u003e\n \u003ctable id=\"Tab3\" border=\"1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eComparison of basic information between all workers at baseline (n\u0026thinsp;=\u0026thinsp;1064) and workers included in the cohort (n\u0026thinsp;=\u0026thinsp;673).\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eBaseline: All workers (n\u0026thinsp;=\u0026thinsp;1064)\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eCohort Inclusion Study Workers (n\u0026thinsp;=\u0026thinsp;673)\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eP\u003c/em\u003e value\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAge (years)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e45.35\u0026thinsp;\u0026plusmn;\u0026thinsp;7.09\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e44.71\u0026thinsp;\u0026plusmn;\u0026thinsp;7.32\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.075\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003egender\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.647\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eman\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e959(90.13)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e602(89.45)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003ewoman\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e105(9.87)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e71(10.55)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eMarital status (yes, N%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.951\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eTie the knot\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1007(94.73)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e638(94.80)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNot married\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e56(5.27)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e35(5.20)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eSmoking (Yes, N%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e671(63.06)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e409(60.77)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.337\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eAlcohol consumption (yes, n%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e447(42.05)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e275(40.86)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.624\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eTea drinking status (yes, n%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e687(64.69)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e425(63.15)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.515\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eNight shift (yes, n%)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e717(67.58)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e467(69.60)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.379\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003ctfoot\u003e\n \u003ctr\u003e\n \u003ctd colspan=\"4\"\u003eNote: Bold represents P\u0026thinsp;\u0026lt;\u0026thinsp;0.05;\u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tfoot\u003e\n \u003c/table\u003e\n \u003c/div\u003e\n \u003cp\u003eThe continuous data were expressed as x\u0026thinsp;\u0026plusmn;\u0026thinsp;s, and the statistical differences were compared by ANOVA.\u003c/p\u003e\n \u003cp\u003eThe numerical data were expressed as n (%), and the chi-square test was used to compare the statistical differences.\u003c/p\u003e\n \u003c/div\u003e\n \u003cdiv id=\"Sec15\" class=\"Section3\"\u003e\n \u003ch2\u003e3.3.2 Association between occupational exposure and biological aging trajectories in workers exposed to polycyclic aromatic hydrocarbons\u003c/h2\u003e\n \u003cp\u003eTable \u003cspan class=\"InternalRef\"\u003e4\u003c/span\u003e shows the results of a multiple logistic regression model of the relationship between urinary OH-PAHs metabolites and accelerated aging trajectories with slow aging as the reference category. The results showed that the odds of being in the moderately accelerated aging increased by 39.2% for each increase of Ʃ-OHPAHs level in urine (OR\u0026thinsp;=\u0026thinsp;1.392; 95%CI: 1.013\u0026thinsp;~\u0026thinsp;1.912), the odds of being in the highly accelerated aging trajectory increased by 61.2% (OR\u0026thinsp;=\u0026thinsp;1.612; 95%CI: 1.093\u0026thinsp;~\u0026thinsp;2.376). For each additional natural logarithmic unit of 1-OHPyr level, the odds of being on a moderately accelerated aging trajectory increased by 47.2% (OR\u0026thinsp;=\u0026thinsp;1.472; 95%CI: 1.058\u0026thinsp;~\u0026thinsp;2.048), the odds of being on a highly accelerated aging trajectory increased by 57.6% (OR\u0026thinsp;=\u0026thinsp;1.576; 95%CI: 1.029\u0026thinsp;~\u0026thinsp;2.413). Similarly, for each additional natural logarithmic unit of 2-OHPhe levels, the odds of being on a highly accelerated aging trajectory increased by 46.7% (OR\u0026thinsp;=\u0026thinsp;1.467; 95%CI: 1.075\u0026thinsp;~\u0026thinsp;2.001).Exposure to 2-OHFLU increasing by one natural logarithmic unit is associated with a lower likelihood of being in the moderately accelerated (OR\u0026thinsp;=\u0026thinsp;0.602, 95% CI: 0.408\u0026ndash;0.887) or highly accelerated trajectory(OR\u0026thinsp;=\u0026thinsp;0.543, 95% CI: 0.334\u0026ndash;0.882) .\u003c/p\u003e\n \u003cdiv class=\"gridtable\"\u003e\n \u003ctable id=\"Tab4\" border=\"1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 4\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eRelationship between Urinary OH-PAHs Metabolites and Aging Trajectories of Occupational Workers\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\" rowspan=\"2\"\u003e\n \u003cp\u003eUrine metabolites of OH-PAHs\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eModerate aging vs. slow aging\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eHigh aging vs. slow aging\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eOR\u003c/em\u003e (95% CI)\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eP\u003c/em\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eOR\u003c/em\u003e (95% CI)\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eP\u003c/em\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eƩ-OHPAHs \u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e1.392(1.013,1.912)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.041\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e1.612(1.093,2.376)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.016\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2-OHNAP \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.112(0.903,1.370)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.316\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.011(0.801,1.276)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.927\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1-OHNAP \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.901(0.672,1.209)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.487\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.957(0.692,1.323)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.790\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3-OHFLU \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.207(0.955,1.526)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.115\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.211(0.926,1.583)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.162\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2-OHFLU \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.602(0.408,0.887)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.010\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.543(0.334,0.882)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.014\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2-OHPHE \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.098(0.882,1.366)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.405\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e1.467(1.075,2.001)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.016\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e9-OHPHE \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.102(0.743,1.634)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.628\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.195(0.757,1.887)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.445\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1-OHPHE \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.109(0.864,1.425)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.417\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.856(0.620,1.180)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.342\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1-OHPYR \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e1.472(1.058,2.048)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.022\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e1.576(1.029,2.413)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.036\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3-OHChr \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.733(0.536,1.003 )\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.052\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.779(0.520,1.166)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.225\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e6-OHChr \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.139(0.894,1.451)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.291\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.964(0.696,1.335)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.825\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e9-OHBap \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.060(0.859,1.308)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.586\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.263(0.945,1.688)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.114\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003ctfoot\u003e\n \u003ctr\u003e\n \u003ctd colspan=\"6\"\u003eNote: OR: odds ratio; CI: Confidence interval. Slow Aging: Slow Aging Trajectory, Moderate Aging: Moderately Accelerated Aging Trajectory, High Aging: Highly Accelerated Aging Trajectory. Model: aAdjust age, gender, education level, smoking status, alcohol consumption status, tea drinking status, exercise status, monthly income, night shift status. bAdjust for age, sex, education, smoking, drinking, tea, exercise, monthly income, night shift, and other urinary OH-PAHs metabolites. Bold indicates P\u0026thinsp;\u0026lt;\u0026thinsp;0.05.\u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tfoot\u003e\n \u003c/table\u003e\n \u003c/div\u003e\n \u003cp\u003eThe 11 ln-transformed OH-PAHs metabolites were categorized into three groups based on their respective P\u003csub\u003e33\u003c/sub\u003e and P\u003csub\u003e66\u003c/sub\u003e percentiles, with biological aging trajectory modeled as a categorical variable. The lowest tertile served as the exposure reference, and slow aging as the outcome reference. Multiple logistic regression analyses demonstrated that subjects in the highest tertile of \u0026Sigma;-OHPAHs and 1-OHPyr were respectively 83.4% (OR\u0026thinsp;=\u0026thinsp;1.834, 95% CI: 1.024\u0026ndash;3.283, P trend\u0026thinsp;=\u0026thinsp;0.034) and 88.6% (OR\u0026thinsp;=\u0026thinsp;1.886, 95% CI: 1.033\u0026ndash;3.444, P trend\u0026thinsp;=\u0026thinsp;0.037) more likely to be on a moderately accelerated aging trajectory compared to the lowest one-third. Those with the highest third of 3-OHChr had a 64.2% decreased risk of being on a moderately accelerated aging trajectory (OR\u0026thinsp;=\u0026thinsp;0.358, 95% CI: 0.143\u0026ndash;0.897), yet the trend test was insignificant. For highly accelerated aging trajectories, subjects in the highest one-third of \u0026Sigma;-OHPAHs, 2-OHPhe, and 1-OHPyr had respective odds of 2.002 (OR\u0026thinsp;=\u0026thinsp;2.002, 95% CI: 1.008\u0026ndash;3.978, P trend\u0026thinsp;=\u0026thinsp;0.04), 3.374 (OR\u0026thinsp;=\u0026thinsp;3.374, 95% CI: 1.133\u0026ndash;10.052, P trend\u0026thinsp;=\u0026thinsp;0.043), and 2.849 (OR\u0026thinsp;=\u0026thinsp;2.849, 95% CI: 1.003\u0026ndash;8.095, P trend\u0026thinsp;=\u0026thinsp;0.043). These results are detailed in Table \u003cspan class=\"InternalRef\"\u003e5\u003c/span\u003e.\u003c/p\u003e\n \u003cdiv class=\"gridtable\"\u003e\n \u003ctable id=\"Tab5\" border=\"1\"\u003e\n \u003ccaption language=\"En\"\u003e\n \u003cdiv class=\"CaptionNumber\"\u003eTable 5\u003c/div\u003e\n \u003cdiv class=\"CaptionContent\"\u003e\n \u003cp\u003eRelationship between urinary OH-PAHs metabolites and aging trajectories of occupational workers\u003c/p\u003e\n \u003c/div\u003e\n \u003c/caption\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth align=\"left\" rowspan=\"2\"\u003e\n \u003cp\u003eUrine metabolites of OH-PAHs\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eModerate aging vs. slow aging\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eHigh aging vs. slow aging\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eOR\u003c/em\u003e (95% CI)\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003eP_trend\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\u0026nbsp;\u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eOR\u003c/em\u003e (95% CI)\u003c/p\u003e\n \u003c/th\u003e\n \u003cth align=\"left\"\u003e\n \u003cp\u003e\u003cem\u003eP_trend\u003c/em\u003e\u003c/p\u003e\n \u003c/th\u003e\n \u003c/tr\u003e\n \u003c/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eƩ-OHPAHs \u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.047(0.615,1.783)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.034\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.239(0.634,2.420)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.043\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e1.834(1.024,3.283)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e2.002(1.008,3.978)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2-OHNAP \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.570(0.299,1.087)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.787\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.397(0.163,0.967)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.439\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.834(0.354,1.964)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.744(0.244,2.263)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1-OHNAP \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.815(0.977,3.371)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.223\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e3.217(1.280,8.086)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.301\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.695(0.818,3.514)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.887(0.698,5.102)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3-OHFLU \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.100(0.551,2.195)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.873\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.184(0.475,2.950)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.976\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.707(0.292,1.711)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.988(0.301,3.243)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2-OHFLU \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.782(0.414,1.478)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.712\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.660(0.274,1.590)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.761\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.688(0.292,1.711)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.367(0.124,1.088)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2-OHPHE \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.983(0.521,1.857)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.101\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.274(0.546,2.972)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.034\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2.000(0.815,4.908)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e3.374(1.133,10.052)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e9-OHPHE \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.478(0.772,2.832)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.296\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.488(0.203,1.174)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.830\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.385(0.652,2.942)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.912(0.342,2.433)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1-OHPHE \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.818(0.414,1.615)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.711\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.686(0.273,1.727)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.075\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.018(0.456,2.271)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.611(0.218,1.717)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1-OHPYR \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.973(0.567,1.668 )\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.037\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.719(0.302,1.713)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.019\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e1.886(1.033,3.444 )\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e2.849(1.003,8.095)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e3-OHChr \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.502(0.239,1.052 )\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.289\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.935(0.360,2.428)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.992\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e0.358(0.143,0.897 )\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.245(0.057,1.048)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e6-OHChr \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.633(0.819,3.256)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.196\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.528(0.583,4.006)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.289\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.901(0.405,2.003)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.617(0.202,1.882)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e9-OHBap \u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT1\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e1.000(Reference)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT2\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2.249(0.981,5.156)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.393\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e2.798(0.961,8.146)\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e0.166\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003eT3\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e3.23(1.259,8.283)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003ctd align=\"left\"\u003e\n \u003cp\u003e\u003cstrong\u003e9.034(1.909,42.753)\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd align=\"left\"\u003e\u0026nbsp;\u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n \u003c/table\u003e\n \u003c/div\u003e\n \u003cp\u003eOR: odds ratio; CI: Confidence interval. Slow Aging: Slow Aging Trajectory, Moderate Aging: Moderately Accelerated Aging Trajectory, High Aging: Highly Accelerated Aging Trajectory. Model: aAdjust age, gender, education level, smoking status, alcohol consumption status, tea drinking status, exercise status, monthly income, night shift status. bAdjust for age, sex, education, smoking, drinking, tea, exercise, monthly income, night shift, and other urinary OH-PAHs metabolites. Bold indicates P\u0026thinsp;\u0026lt;\u0026thinsp;0.05.\u003c/p\u003e\n \u003c/div\u003e\n\u003c/div\u003e"},{"header":"4. Discussion","content":"\u003cp\u003eThis study undertook the development of a biological age predictor for Chinese workers and assessed the correlation between PAH exposure and aging biomarkers.In a prospective cohort study, the research successfully applied group-based trajectory modeling (GBTM) techniques to identify three distinct aging trajectories within the study sample, thereby evaluating the rate of aging. Biological age, as a more precise measure of aging than chronological age, integrates various biomarkers related to physical function and provides comprehensive information about overall health. The results indicate that individuals exposed to high levels of PAHs tended to be biologically older than those exposed to low levels. Specifically, each natural logarithmic unit increase in Σ-OHPAHs raised the likelihood of moderate and high accelerated aging trajectories by 39.2% and 61.2%, respectively.\u003c/p\u003e\u003cp\u003ePAHs, comprising hundreds of chemically related compounds with diverse structures and toxicities, including carcinogenicity and neurotoxicity, persistently pollute the environment. Exposure in occupational environments may involve multiple pathways, affecting the total absorbed dose (e.g.skin and inhalation exposure to contaminated air)\u003csup\u003e\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e,\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e\u003c/sup\u003e. People can be exposed to PAHs through direct inhalation, ingestion, or skin contact with air and surface soils containing these compounds\u003csup\u003e\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e\u003c/sup\u003e. Workers in coking plants are commonly exposed to PAHs. This study selected the content of PAH metabolites in urine as an indicator of human exposure, primarily based on its high specificity as a biomarker compared to blood samples, as well as the non-invasive collection advantages of urine samples.Given the complexity of PAHs in coking plants, 11 PAH hydroxy metabolites were used as a comprehensive exposure index.\u003c/p\u003e\u003cp\u003eHuman aging likely begins at or before reproductive maturity Identifying modifiable risk factors is crucial for maintaining health and quality of life during aging\u003csup\u003e\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e\u003c/sup\u003e. Chronological Age (CA) serves as a convenient method for assessing the aging status of individuals, but it cannot accurately reflect the differences in aging rates among individuals within the same CA group. The variation in cognitive function and health status increases with the growth of CA, indicating that CA may not accurately reveal the true aging differences between individuals.The proposed biological age (BA) describes aging relative to CA\u003csup\u003e23\u003c/sup\u003e.\u003c/p\u003e\u003cp\u003eThe estimation of biological age (BA) can be facilitated by omics indicators (such as telomere length)\u003csup\u003e\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e\u003c/sup\u003e, clinical indicators(BA measurement constructed based on the Klemera and Doubal method)\u003csup\u003e\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e\u003c/sup\u003e, and phenotypic indicators (such as frail phenotype, Frailty Index (FI), and functional aging index)\u003csup\u003e\u003cspan additionalcitationids=\"CR27\" citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e\u003c/sup\u003e.This study employed a BA method constructed based on clinical indicators, which has the advantage of using chronological age (CA) as a standard biomarker in the model, significantly enhancing the accuracy of BA estimation, and elucidating the known \"biomarker paradox\" correlation. The study demonstrated that incorporating CA as a biomarker into the model significantly reduced the standard error of BA estimation. In predicting mortality and disease, the KDM method has been proven superior to other traditional methods\u003csup\u003e\u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e\u003c/sup\u003e. In 2020, a longitudinal cohort study of Singaporean Chinese compared the differences in BA prediction of aging, disease, and mortality using MLR, PCA, and KDM methods\u003csup\u003e\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e\u003c/sup\u003e. The results showed that the KDM method's BA had significant predictive power in the younger group, and for groups with the same CA, the group with accelerated aging had a higher incidence of disease, worse health conditions, and more severe biological functional aging than the group with slower aging. This study applied the KDM method to construct BA in the population of Chinese occupational workers, and the results showed that KDM-BA highly matched with CA. In our study, the constructed KDM-BA included biochemical markers of liver function, kidney function, cardiovascular function, metabolic function, lipids, inflammation, and metabolism, which allows us to more comprehensively identify changes in the workers' bodies and distinguish earlier those in a preclinical state.Studies have shown that even at baseline without ischemic heart disease, stroke, or cancer, KDM-Accel to some extent distinguished individuals who may be in the subclinical stage of cardiovascular disease (CVD), indicating that KDM-Accel can reflect preclinical content earlier and better reflect early changes in the human body.\u003c/p\u003e\u003cp\u003eEnvironmental factors, including air pollution, have a potential impact on the biological aging process. Long-term exposure to air pollution is considered one of the key factors leading to adverse age-related outcomes, particularly cardiovascular diseases (CVD) and respiratory system diseases\u003csup\u003e\u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e31\u003c/span\u003e\u003c/sup\u003e.Studies have shown that exposure to air pollution may even shorten the expected lifespan of individuals with good genetic endowment. Polycyclic aromatic hydrocarbons (PAHs) cause mitochondrial dysfunction, oxidative stress\u003csup\u003e\u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e32\u003c/span\u003e\u003c/sup\u003e, and DNA damage\u003csup\u003e\u003cspan citationid=\"CR33\" class=\"CitationRef\"\u003e33\u003c/span\u003e\u003c/sup\u003e, thereby leading to cellular aging and premature bodily aging\u003csup\u003e\u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e34\u003c/span\u003e\u003c/sup\u003e. Oxidative stress and the resulting chronic inflammation have been confirmed as significant pathogenic factors for the aging process and age-related diseases\u003csup\u003e\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e\u003c/sup\u003e.This study for the first time reveals the correlation between high exposure to PAHs and accelerated biological aging. Specifically, for each additional natural logarithmic unit of Ʃ-OHPAHs, the biological aging indicator of workers increases by 0.029 years; for each additional natural logarithmic unit of 1-OHPYR concentration, the biological aging indicator KDM-Accel increases by 0.028 years; for each additional natural logarithmic unit of 2-OHPHE concentration, the biological aging indicator KDM-Accel increases by 0.017 years. The results of this study are consistent with previous studies that workers occupationally exposed to PAHs exhibit shorter telomere lengths and accelerated lymphocyte DNA methylation age compared to those exposed to less PAHs\u003csup\u003e\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u003c/sup\u003e; long-term skin exposure to PAHs may lead to higher skin aging scores\u003csup\u003e\u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e35\u003c/span\u003e\u003c/sup\u003e; 1-OHPYR and 9-OHPHE are associated with methylation aging independently of other OH-PAHs and risk factors\u003csup\u003e\u003cspan citationid=\"CR36\" class=\"CitationRef\"\u003e36\u003c/span\u003e\u003c/sup\u003e. Furthermore, this study has several differences. First, we used objectively measured urinary OH-PAH metabolites to quantify the total exposure to PAHs from various sources, including diet, cooking, smoking, heating, transportation, waste burning, and occupational exposure. The use of OH-PAH metabolites not only improves the accuracy of exposure measurement but also allows us to distinguish the effects of different types of PAHs. Furthermore, among our study participants,we did not observe a significant correlation between smoking and biological aging; this observation may be partly due to the fact that smoking largely leads to exposure to naphthalene rather than phenanthrene and pyrene\u003csup\u003e\u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e37\u003c/span\u003e\u003c/sup\u003e. Second, compared to previous cellular aging indicators, biological age provides additional information\u0026mdash;it has a similar scale to actual age and can be linked to the epigenetic behavior of certain age-related genes\u003csup\u003e\u003cspan citationid=\"CR38\" class=\"CitationRef\"\u003e38\u003c/span\u003e\u003c/sup\u003e. After multiple logistic analyses by gender grouping, the results showed that the male group was consistent with the total population(Table S8). However, in females, only 2-OHFLU showed consistent results, while Ʃ-OHPAHs were not statistically significant for the aging trajectory(Table S9). One possible reason is that estrogen has antioxidant and anti-inflammatory effects, and the biological aging caused by PAH exposure is through oxidative stress and chronic inflammation, which leads to a smaller impact of PAHs on the biological aging indicators of females\u003csup\u003e\u003cspan citationid=\"CR39\" class=\"CitationRef\"\u003e39\u003c/span\u003e\u003c/sup\u003e. Another important factor is that after stratification by gender, there were fewer female samples, and therefore, the impact of PAHs on the biological aging indicators of the female group could not be observed.\u003c/p\u003e\u003cp\u003eRegarding the trajectory of aging, early studies identified different patterns of human aging trajectories by measuring indices such as frailty index\u003csup\u003e\u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e40\u003c/span\u003e\u003c/sup\u003e, health and functional items\u003csup\u003e\u003cspan citationid=\"CR41\" class=\"CitationRef\"\u003e41\u003c/span\u003e,\u003cspan citationid=\"CR42\" class=\"CitationRef\"\u003e42\u003c/span\u003e\u003c/sup\u003e, physical capacity, and educational items\u003csup\u003e\u003cspan citationid=\"CR43\" class=\"CitationRef\"\u003e43\u003c/span\u003e\u003c/sup\u003e, or other subjective indicators\u003csup\u003e\u003cspan citationid=\"CR44\" class=\"CitationRef\"\u003e44\u003c/span\u003e\u003c/sup\u003e. Compared with previous studies, our current research demonstrates the feasibility of long-term estimation of aging speed in the population of Chinese occupational workers. Compared to measuring aging at a single time point, the biological aging trajectory established based on aging biomarkers measured over a longer period may better capture the dynamics and heterogeneous nature of aging and is expected to accurately identify different populations with varying risks of age-related diseases or mortality. In the study, it was found that as the time of enrollment increased, the trajectory curve gradually rose. A possible explanation is that our population consists of workers under the age of 60, and most people experience a certain degree of chronic diseases around the age of 60\u003csup\u003e45\u003c/sup\u003e, with exposure to PAHs increasing the risk of such diseases. This will lead to an increasing trend in biological age over time for our study subjects. Previous studies constructed biological aging trajectories based on functional decline indicators, and the results showed that with the increase of time, the aging trajectory presented an upward trend, which is consistent with our study. We reconstructed the biological aging trajectory charts for coking plant workers and the control group of water treatment plant workers, and consistent results were found, both showing an increasing trend. Moreover, the aging trajectory chart constructed for coking plant workers showed a higher increase trend and a steeper slope than the aging trajectory of the control group water treatment plant, indicating that the biological aging trajectory may be caused by exposure to PAHs, resulting in a higher increase trend. Our results show that for every natural logarithmic unit increase in the urinary level of Σ-OHPAHs, the likelihood of being in a moderately accelerated aging trajectory increases by 39.2% (OR\u0026thinsp;=\u0026thinsp;1.392; 95%CI: 1.013\u0026thinsp;~\u0026thinsp;1.912), and the likelihood of being in a highly accelerated aging trajectory increases by 61.2% (OR\u0026thinsp;=\u0026thinsp;1.612; 95%CI: 1.093\u0026thinsp;~\u0026thinsp;2.376). For every natural logarithmic unit increase in 1-OHPyr, the likelihood of being in a moderately accelerated aging trajectory increases by 47.2% (OR\u0026thinsp;=\u0026thinsp;1.472; 95%CI: 1.058\u0026thinsp;~\u0026thinsp;2.048), and the likelihood of being in a highly accelerated aging trajectory increases by 57.6% (OR\u0026thinsp;=\u0026thinsp;1.576; 95%CI: 1.029\u0026thinsp;~\u0026thinsp;2.413). Additionally, for every natural logarithmic unit increase in 2-OHPHE, the likelihood of being in a highly accelerated aging trajectory increases by 46.7% (OR\u0026thinsp;=\u0026thinsp;1.467; 95%CI: 1.075\u0026thinsp;~\u0026thinsp;2.001). We have further confirmed from the perspective of trajectories that PAHs can accelerate the process of biological aging. Although accelerated aging is a multi-system aging process with complex underlying biological mechanisms, our findings may provide an exciting new approach to studying how environmental pollution induces adverse health outcomes related to aging (including age-related diseases and mortality) through aging, offering more public health significance for the prevention of diseases in occupational workers.\u003c/p\u003e\u003cp\u003eOur study has several advantages. Firstly, we conducted a prospective cohort study. Secondly, we conceptualized biological aging acceleration using multiple indicators, which are based on 12 biomarkers from routine examinations representing multiple organ systems. Thirdly, compared to a single time point assessment, we tracked aging acceleration at five time points using Group-Based Trajectory Modeling (GBTM), which may be more suitable for evaluating the aging process. The GBTM model explains the changes over time, differentiates the acceleration of aging over time, and is capable of identifying the heterogeneity of aging.\u003c/p\u003e\u003cp\u003e\u003cb\u003eStudy limitations\u003c/b\u003e\u003c/p\u003e\u003cp\u003eHowever, this study has certain limitations. Firstly, we only conducted a single measurement of polycyclic aromatic hydrocarbons (PAHs) in urine, but since urinary PAH metabolites can reflect long-term internal exposure indicators, PAHs have been used as internal exposure indicators in previous studies. Secondly, we tried to control all confounding factors, but due to the observational nature of the study, the possibility of residual confounding cannot be ruled out. Thirdly, because our correlation analysis was conducted in a cohort of occupational workers, biological aging was only measured in whole blood, so our correlation results may not be generalizable to other non-worker populations or to biological aging in other tissues. Fourthly, our cohort study had a relatively short duration, allowing us to observe only the short-term relationship between PAHs and biological aging trajectories. Lastly, our study population had a relatively small proportion of females. We plan to increase the number of female subjects in future studies to observe the impact of PAHs on biological aging in females.\u003c/p\u003e"},{"header":"5. Conclusion","content":"\u003cp\u003eIn summary, our study provides evidence that workers with higher exposure to polycyclic aromatic hydrocarbons (PAHs) experience accelerated aging compared to those with lower exposure. PAHs levels are positively associated with biological aging indicators and trajectories. Occupational PAHs exposure may predispose workers to accelerated aging. Reducing PAHs exposure could potentially reduce the aging rate among coke oven workers. Future longitudinal studies should employ larger samples with broader demographic representation to validate these findings and develop targeted interventions against occupationally accelerated aging.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eCorresponding author\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eJisheng Nie, MD, MPH, PhD. E-mail: [email protected]\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eData availability\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe data that support the findings of this study are not publicly available due to their containing information that could compromise the privacy of research participants. The data are available from the corresponding author upon reasonable request.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthor contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWYD and NJS conceptualized the research question. GSX, WWY and YLJ acquired the data.WYD and GSX performed all the analyses and visualized the results. WYD drafted the manuscript.GSX,ZHF,PBL and NJS helped with the interpretation and provided critical review. NJS, and NQ supervised all aspects of the project. All\u003c/p\u003e\n\u003cp\u003ethe authors read and approved the final manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgement\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe thank the participants and General Hospital of Taiyuan Iron \u0026amp; Steel (Group) Co., Ltd, Taiyuan, China, who assisted with the sample collection and Montreal Cognitive Assessment.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis study has been supported by the National Natural Science Foundation of China (No. 82073526 and No.81673143).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eDeclaration of competing interest\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eEthics approval and consent to participate\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis study was performed in accordance with the Declaration of Helsinki. The study protocol was approved by the Medical Ethics Committee of Shanxi Medical University (Approval No. 2020GLL037). Written informed consent was obtained from all individual participants included in the study.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eStec, A. 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H.\u003cem\u003e et al.\u003c/em\u003e Predicting Trajectories of Functional Decline in 60- to 70-Year-Old People. \u003cem\u003eGerontology\u003c/em\u003e \u003cstrong\u003e64\u003c/strong\u003e, 212-221 (2018). https://doi.org:10.1159/000485135\u003c/li\u003e\n\u003cli\u003eCheng, X.\u003cem\u003e et al.\u003c/em\u003e Population ageing and mortality during 1990-2017: A global decomposition analysis. \u003cem\u003ePLoS Med\u003c/em\u003e \u003cstrong\u003e17\u003c/strong\u003e, e1003138 (2020). https://doi.org:10.1371/journal.pmed.1003138\u003c/li\u003e\n\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Polycyclic aromatic hydrocarbons, Occupational exposure, Biological age, Aging trajectory, Prospective cohort study","lastPublishedDoi":"10.21203/rs.3.rs-7945721/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7945721/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eBackground: \u003c/strong\u003eLong-term effects of occupational polycyclic aromatic hydrocarbons (PAHs) on biological aging are unclear.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethods: \u003c/strong\u003eA prospective cohort study was conducted from 2019 to 2023, involving 610 coke oven workers and 454 control workers from a water treatment plant. Biological age was calculated using the Klemera and Doubal method (KDM-BA) based on 12 clinical biomarkers, and aging acceleration (KDM-Accel) was derived. Group-based trajectory modeling (GBTM) was employed to identify distinct aging trajectories over five time points. The associations between eleven urinary mono-hydroxylated PAH metabolites (measured via HPLC-MS) and both KDM-Accel and aging trajectories were assessed using multiple linear regression and multivariable logistic regression, respectively.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults: \u003c/strong\u003eGBTM identified three distinct aging trajectories: slow aging (14.86%), moderately accelerated aging (59.14%), and rapidly accelerated aging (26%). Higher concentrations of urinary Σ-OHPAHs (sum of all metabolites), 1-hydroxypyrene (1-OHPyr), and 2-hydroxyphenanthrene (2-OHPhe) were significantly associated with increased KDM-Accel. A natural log-unit increase in Σ-OHPAHs and 1-OHPyr was associated with a 0.029-year and 0.028-year increase in KDM-Accel, respectively. Workers in the highest tertile (T3) of Σ-OHPAHs exposure had a 61.2% increased odds (OR = 1.612, 95% CI: 1.093–2.376) of being in the rapidly accelerated aging trajectory compared to those in the lowest tertile (T1). Similar positive dose-response relationships were observed.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusion: \u003c/strong\u003eOccupational PAH exposure accelerates biological aging ; enhanced protection and early intervention are warranted.\u003c/p\u003e","manuscriptTitle":"Occupational polycyclic aromatic hydrocarbons (PAHs) exposure and aging trajectory : Epidemiologic evidence from a cohort of Chinese coke oven workers (2019-2023)","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-11-21 05:13:24","doi":"10.21203/rs.3.rs-7945721/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-12-15T04:27:39+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-12-12T03:21:01+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-12-03T09:38:21+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"117851506362555918079055409330260723397","date":"2025-11-28T02:02:24+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"165804987337330867604164080635470176368","date":"2025-11-17T03:53:32+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-11-11T16:25:33+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-11-11T16:22:03+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2025-11-03T04:27:51+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-10-29T14:14:24+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2025-10-29T14:10:07+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"347f2e0b-cf8f-423e-9f99-543b86d6a655","owner":[],"postedDate":"November 21st, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":58080745,"name":"Health sciences/Biomarkers"},{"id":58080746,"name":"Earth and environmental sciences/Environmental sciences"},{"id":58080747,"name":"Health sciences/Health care"},{"id":58080748,"name":"Health sciences/Medical research"},{"id":58080749,"name":"Health sciences/Risk factors"}],"tags":[],"updatedAt":"2026-02-02T16:00:33+00:00","versionOfRecord":{"articleIdentity":"rs-7945721","link":"https://doi.org/10.1038/s41598-026-36579-y","journal":{"identity":"scientific-reports","isVorOnly":false,"title":"Scientific Reports"},"publishedOn":"2026-01-31 15:58:16","publishedOnDateReadable":"January 31st, 2026"},"versionCreatedAt":"2025-11-21 05:13:24","video":"","vorDoi":"10.1038/s41598-026-36579-y","vorDoiUrl":"https://doi.org/10.1038/s41598-026-36579-y","workflowStages":[]},"version":"v1","identity":"rs-7945721","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7945721","identity":"rs-7945721","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}