Cloning and Functional Verification of Gene GmELF4-LIKE4 in Soybean [Glycine max (L.) Merr]

Cloning and Functional Verification of Gene GmELF4-LIKE4 in Soybean [Glycine max (L.) Merr] | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research article Cloning and Functional Verification of Gene GmELF4-LIKE4 in Soybean [Glycine max (L.) Merr] Yukun Jin, He Zhao, Abraham Lamboro, Zhifeng Xiao, Qi Zhang, Shuyan Guan, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-126101/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 7 You are reading this latest preprint version Abstract Background Soybean grain is an important oil crop with high-quality vegetable protein and vegetable oil. Extreme weather can cause crop yield reduction, among which drought is most likely to cause a decline in annual soybean yield. How to overcome the impact of drought on soybean yield has become a major work in current breeding research. Results In our study, the gene GmELF4-LIKE4 was obtained through RNA-Seq and differential gene screening technology, and the plant over expression vector and RNAi vector were constructed. Then, the Colombian Arabidopsis thaliana and soybean JN18 were genetically transformed and added to the Agrobacterium-mediated method in to T2 generation. We have verified in Arabidopsis thaliana that over expression of ELF4-LIKE4 will delay the flowering of Arabidopsis thaliana, while inhibition of ELF4-LIKE4 expression will advance the flowering period. At the same time, we verified the regulation analysis of GmELF4-LIKE4 on EARLY FLOWERING 3 and CONSTANTS-like in soybeans, and found that GmELF4-LIKE4 positively regulates ELF3 and COL; meanwhile, over expression soybeans and RNAi soybeans were tested in drought. Stressing the relative conductivity, malondialdehyde content and peroxidase activity under 0 days and 5 days, the result indicate that the over expression of GmELF4-LIKE4 gene can reduce the drought resistance of soybean. This study laid a theoretical foundation for the identification of GmELF4-LIKE4 gene function and the breeding of drought-resistant varieties. Conclusion We found that overexpression of GmELF4-LIKE4 will delay the flowering period of Arabidopsis thaliana and reduce the drought resistance of soybeans. Conversely, inhibiting GmELF4-LIKE4 will advance the flowering of Arabidopsis thaliana and increase the drought resistance of soybeans. Biotechnology and Bioengineering Soybean RNA-Seq GmELF4-LIKE4 Cloning Drought identification Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Full Text Supplementary Files renamed766c1.pdf Cite Share Download PDF Status: Under Review Version 1 posted Review # 1 received at journal 06 Apr, 2021 Reviewer # 1 agreed at journal 28 Mar, 2021 Reviewers invited by journal 21 Dec, 2020 Editor assigned by journal 16 Dec, 2020 Submission checks completed at journal 10 Dec, 2020 Editor invited by journal 07 Dec, 2020 First submitted to journal 24 Nov, 2020 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-126101","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research article","associatedPublications":[],"authors":[{"id":6241670,"identity":"ff9a7eab-6966-4010-a79a-d84fe44ccbb2","order_by":0,"name":"Yukun Jin","email":"","orcid":"https://orcid.org/0000-0002-4739-863X","institution":"Jilin Agricultural University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Yukun","middleName":"","lastName":"Jin","suffix":""},{"id":6241671,"identity":"51feaac7-5d7b-4640-aa6b-36efd7362b21","order_by":1,"name":"He Zhao","email":"","orcid":"","institution":"Jilin Agricultural 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Elongation culture; F: Rooting culture; G: Regenerated plants","description":"","filename":"4.JPG","url":"https://assets-eu.researchsquare.com/files/rs-126101/v1/df59b904f93986af91ec1310.JPG"},{"id":4282314,"identity":"520245aa-d87b-4167-9f7e-aeadcabae907","added_by":"auto","created_at":"2020-12-15 19:47:03","extension":"jpg","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":65259,"visible":true,"origin":"","legend":"Analysis of relative expression of GmELF4-LIKE4 in tissues of transgenic soybean A: Analysis of Relative Expression of Over expressed soybean in Roots\nB: Analysis of Relative Expression of Over expressed soybean in Leaves； C: Analysis of Relative Expression of Over expressed soybean in Flowers； D: Analysis of Relative Expression of RNAi soybean in Roots；\nE: Analysis of Relative Expression of RNAi soybean in Leaves； F: Analysis of Relative Expression of RNAi soybean in 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Merr]\u003c/p\u003e","fulltext":[{"header":"Full Text","content":"\u003cp\u003eThis preprint is available for \u003ca href='/article/rs-126101/latest.pdf' target='_blank'\u003edownload as a PDF\u003c/a\u003e.\u003c/p\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"bmc-biotechnology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bbit","sideBox":"Learn more about [BMC Biotechnology](http://bmcbiotechnol.biomedcentral.com/)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/bbit/default.aspx","title":"BMC Biotechnology","twitterHandle":"BMC_series","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"em","reportingPortfolio":"BMC Series","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Soybean, RNA-Seq, GmELF4-LIKE4, Cloning, Drought identification","lastPublishedDoi":"10.21203/rs.3.rs-126101/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-126101/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eBackground\u003c/p\u003e\u003cp\u003eSoybean grain is an important oil crop with high-quality vegetable protein and vegetable oil. Extreme weather can cause crop yield reduction, among which drought is most likely to cause a decline in annual soybean yield. How to overcome the impact of drought on soybean yield has become a major work in current breeding research.\u003c/p\u003e\u003cp\u003eResults\u003c/p\u003e\u003cp\u003eIn our study, the gene GmELF4-LIKE4 was obtained through RNA-Seq and differential gene screening technology, and the plant over expression vector and RNAi vector were constructed. Then, the Colombian Arabidopsis thaliana and soybean JN18 were genetically transformed and added to the Agrobacterium-mediated method in to T2 generation. We have verified in Arabidopsis thaliana that over expression of ELF4-LIKE4 will delay the flowering of Arabidopsis thaliana, while inhibition of ELF4-LIKE4 expression will advance the flowering period. At the same time, we verified the regulation analysis of GmELF4-LIKE4 on EARLY FLOWERING 3 and CONSTANTS-like in soybeans, and found that GmELF4-LIKE4 positively regulates ELF3 and COL; meanwhile, over expression soybeans and RNAi soybeans were tested in drought. Stressing the relative conductivity, malondialdehyde content and peroxidase activity under 0 days and 5 days, the result indicate that the over expression of GmELF4-LIKE4 gene can reduce the drought resistance of soybean. This study laid a theoretical foundation for the identification of GmELF4-LIKE4 gene function and the breeding of drought-resistant varieties.\u003c/p\u003e\u003cp\u003eConclusion\u003c/p\u003e\u003cp\u003eWe found that overexpression of GmELF4-LIKE4 will delay the flowering period of Arabidopsis thaliana and reduce the drought resistance of soybeans. Conversely, inhibiting GmELF4-LIKE4 will advance the flowering of Arabidopsis thaliana and increase the drought resistance of soybeans.\u003c/p\u003e","manuscriptTitle":"Cloning and Functional Verification of Gene GmELF4-LIKE4 in Soybean\u0026nbsp;[Glycine max (L.) 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