Comparison of rhizosphere microbial community of Panax notoginseng under different habitat

Comparison of rhizosphere microbial community of Panax notoginseng under different habitat | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Comparison of rhizosphere microbial community of Panax notoginseng under different habitat can wang, hongwei sun, dawei zhao, yuling yang, ling li, qingsong yang, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4487651/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract This study compared and analyzed the diversity differences of soil microbial communities in the rhizosphere of Panax notoginseng under different habitats (under forest and field) and their correlation with environmental factors. Illumina MiSeq high-throughput sequencing revealed that the diversity index of rhizosphere bacterial communities in P. notoginseng under forest (Ace, Chao1, Simpson, Shannon) was significantly higher than in field. In comparison to the microbial networks observed in the field (Nodes=186, Edges=3407), the microbial networks found in the underforest (Nodes=266, Edges=4549) demonstrate a more intricate and sophisticated correlation among microorganisms. The results revealed that organic carbon emerged as the primary main influencing the assembly of rhizosphere bacterial communities in P. notoginseng under forest, accounting for 84.23% of the observed community variation. However, in fungi, the pH (75.10 %) value assumes significance. Moreover, the total saponin content of P. notoginseng in the forest was significantly higher compared to that in the field, particularly with regards to saponin R1, which exhibited a significant association with the microbial community present under forest conditions. Biological sciences/Microbiology Biological sciences/Molecular biology Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementFigures.doc SupplementTable.doc Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4487651","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":314016720,"identity":"d22e75ca-55fe-441f-8222-0cbf046223d2","order_by":0,"name":"can wang","email":"","orcid":"","institution":"Institute of Medicinal Biological Technique, Wenshan Academy of Agricultural Sciences","correspondingAuthor":false,"prefix":"","firstName":"can","middleName":"","lastName":"wang","suffix":""},{"id":314016721,"identity":"7cb1f2ca-aeb6-4584-a386-0792f4c68fd6","order_by":1,"name":"hongwei sun","email":"","orcid":"","institution":"Institute of Medicinal Biological Technique, Wenshan Academy of Agricultural Sciences","correspondingAuthor":false,"prefix":"","firstName":"hongwei","middleName":"","lastName":"sun","suffix":""},{"id":314016722,"identity":"055a5ca3-c573-4541-8f65-681e2d1b7086","order_by":2,"name":"dawei zhao","email":"","orcid":"","institution":"Institute of Medicinal Biological Technique, Wenshan Academy of Agricultural Sciences","correspondingAuthor":false,"prefix":"","firstName":"dawei","middleName":"","lastName":"zhao","suffix":""},{"id":314016723,"identity":"d5bee299-4cb8-4ae1-9eb8-5d067c126ed1","order_by":3,"name":"yuling yang","email":"","orcid":"","institution":"Institute of Medicinal Biological Technique, Wenshan Academy of Agricultural Sciences","correspondingAuthor":false,"prefix":"","firstName":"yuling","middleName":"","lastName":"yang","suffix":""},{"id":314016724,"identity":"b70817c9-8a49-48e5-947b-82c172d8333f","order_by":4,"name":"ling li","email":"","orcid":"","institution":"Institute of Medicinal Biological Technique, Wenshan Academy of Agricultural Sciences","correspondingAuthor":false,"prefix":"","firstName":"ling","middleName":"","lastName":"li","suffix":""},{"id":314016725,"identity":"8363c603-9e72-42c7-9ddc-495ca9d6e6f1","order_by":5,"name":"qingsong yang","email":"","orcid":"","institution":"Institute of Medicinal Biological Technique, Wenshan Academy of Agricultural Sciences","correspondingAuthor":false,"prefix":"","firstName":"qingsong","middleName":"","lastName":"yang","suffix":""},{"id":314016726,"identity":"21493c5e-8b6c-43ea-b6f5-9b2a2b34a457","order_by":6,"name":"daju chen","email":"","orcid":"","institution":"Institute of Medicinal Biological Technique, Wenshan Academy of Agricultural Sciences","correspondingAuthor":false,"prefix":"","firstName":"daju","middleName":"","lastName":"chen","suffix":""},{"id":314016727,"identity":"b1f63e50-9b53-4b19-a82b-49f30f711823","order_by":7,"name":"yonghan qu","email":"","orcid":"","institution":"Institute of Medicinal Biological Technique, Wenshan Academy of Agricultural Sciences","correspondingAuthor":false,"prefix":"","firstName":"yonghan","middleName":"","lastName":"qu","suffix":""},{"id":314016729,"identity":"aa7a3f88-fa90-4fab-b58f-1e06815e5d06","order_by":8,"name":"qing yang","email":"","orcid":"","institution":"Institute of Medicinal Biological Technique, Wenshan Academy of Agricultural Sciences","correspondingAuthor":false,"prefix":"","firstName":"qing","middleName":"","lastName":"yang","suffix":""},{"id":314016731,"identity":"e2db0c90-8daa-46a8-99af-1bd8a918e577","order_by":9,"name":"cuixian peng","email":"","orcid":"","institution":"Institute of Medicinal Biological Technique, Wenshan Academy of Agricultural Sciences","correspondingAuthor":false,"prefix":"","firstName":"cuixian","middleName":"","lastName":"peng","suffix":""},{"id":314016733,"identity":"142c7d23-a850-494d-9d77-ba695b84a264","order_by":10,"name":"yonghong tao","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAxklEQVRIiWNgGAWjYJACZjDJ3sBwgCjlPHAtPAdI1iKRQKSj7NkPH5MuqLGTl5/5/OHhghoGeXOCtvCkpUnPOJZsuOF2jsHhGccYDHc2EHRYjpk0bwMz4wbpHIbDPGwMCQYHCGnhfwPSUm8/f+bxB4d5/hGjRQJsy+HEhhsMBod524jRcuNZsjXPsePJG84A/cLbJ2G4gZAW9v7kg7d5aqpt57cff/yZ55uNPEFbgIBFAokjgVMZMmD+QJSyUTAKRsEoGLkAACu1PHwcQ8ztAAAAAElFTkSuQmCC","orcid":"","institution":"Institute of Medicinal Biological Technique, Wenshan Academy of Agricultural Sciences","correspondingAuthor":true,"prefix":"","firstName":"yonghong","middleName":"","lastName":"tao","suffix":""}],"badges":[],"createdAt":"2024-05-28 02:47:49","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4487651/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4487651/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":61331430,"identity":"2003adf7-f126-4467-9da7-ef551f232704","added_by":"auto","created_at":"2024-07-29 14:54:54","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":846458,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript6.1.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4487651/v1_covered_3b8860a8-e293-4f0e-809f-0c44fb6aec1e.pdf"},{"id":58390690,"identity":"7cfb6fcb-0220-453a-b733-eedfa6882a44","added_by":"auto","created_at":"2024-06-14 20:20:47","extension":"doc","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":1569280,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementFigures.doc","url":"https://assets-eu.researchsquare.com/files/rs-4487651/v1/e1586200ee23558572e66d0c.doc"},{"id":58390689,"identity":"396324a7-79ac-45b6-9716-ee703a941f27","added_by":"auto","created_at":"2024-06-14 20:20:47","extension":"doc","order_by":2,"title":"","display":"","copyAsset":false,"role":"supplement","size":44032,"visible":true,"origin":"","legend":"","description":"","filename":"SupplementTable.doc","url":"https://assets-eu.researchsquare.com/files/rs-4487651/v1/ac8663c44f2f82eefb1f9034.doc"}],"financialInterests":"No competing interests reported.","formattedTitle":"Comparison of rhizosphere microbial community of Panax notoginseng under different habitat","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-4487651/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4487651/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"This study compared and analyzed the diversity differences of soil microbial communities in the rhizosphere of Panax notoginseng under different habitats (under forest and field) and their correlation with environmental factors. Illumina MiSeq high-throughput sequencing revealed that the diversity index of rhizosphere bacterial communities in P. notoginseng under forest (Ace, Chao1, Simpson, Shannon) was significantly higher than in field. In comparison to the microbial networks observed in the field (Nodes=186, Edges=3407), the microbial networks found in the underforest (Nodes=266, Edges=4549) demonstrate a more intricate and sophisticated correlation among microorganisms. The results revealed that organic carbon emerged as the primary main influencing the assembly of rhizosphere bacterial communities in P. notoginseng under forest, accounting for 84.23% of the observed community variation. However, in fungi, the pH (75.10 %) value assumes significance. Moreover, the total saponin content of P. notoginseng in the forest was significantly higher compared to that in the field, particularly with regards to saponin R1, which exhibited a significant association with the microbial community present under forest conditions. ","manuscriptTitle":"Comparison of rhizosphere microbial community of Panax notoginseng under different habitat","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-06-14 20:20:42","doi":"10.21203/rs.3.rs-4487651/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"049c68d7-c514-4c5b-b630-428011705a4c","owner":[],"postedDate":"June 14th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":33207824,"name":"Biological sciences/Microbiology"},{"id":33207825,"name":"Biological sciences/Molecular biology"}],"tags":[],"updatedAt":"2024-07-29T14:46:46+00:00","versionOfRecord":[],"versionCreatedAt":"2024-06-14 20:20:42","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-4487651","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4487651","identity":"rs-4487651","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}