Cryo-EM Structure of the LARS1-IARS1 Complex Reveals A Nutrient-Responsive Switch Controlling mTORC1 Signaling

Cryo-EM Structure of the LARS1-IARS1 Complex Reveals A Nutrient-Responsive Switch Controlling mTORC1 Signaling | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Cryo-EM Structure of the LARS1-IARS1 Complex Reveals A Nutrient-Responsive Switch Controlling mTORC1 Signaling Hee-Sung Park, Youjin Kim, Joo-Chan Kim, Do-Wook Kim, Jinwoo Kim, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7316280/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted You are reading this latest preprint version Abstract Aminoacyl-tRNA synthetases (ARSs) assemble into the multi-tRNA synthetase complex (MSC) to mediate noncanonical functions in cell signaling and stress responses. Among them, leucyl-tRNA synthetase 1 (LARS1) plays a central role in sensing leucine and activating mTORC1, thereby linking nutrient availability to metabolic regulation. Despite recent progress, fundamental questions remain unresolved about the regulatory mechanisms governing LARS1’s non-canonical functions, particularly its structural organization within the MSC. Here, we employed an integrated approach combining structural, biochemical, and cellular analyses to investigate the noncanonical roles of LARS1. The cryo-EM structure of the LARS1:Isoleucyl-tRNA synthetase 1 (IARS1) complex revealed that LARS1 binds to IARS1, its anchoring partner in the MSC, via its UNE-L domain. Amino acid stimulation induces LARS1 phosphorylation at Ser1070, Ser1077, and Ser1082, which are located at the interface with IARS1. These modifications disrupt the interaction, promote LARS1 dissociation from the MSC, and enable mTORC1 activation. This study highlights phosphorylation as a conserved and critical molecular switch that orchestrates the non-canonical functions of MSC by dynamically modulating the assembly and activity of its components in response to external stimuli. Biological sciences/Biochemistry/Structural biology/Electron microscopy/Cryoelectron microscopy Biological sciences/Cell biology/Cell signalling/Nutrient signalling Biological sciences/Biochemistry/Proteins/Intracellular signalling peptides and proteins Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 1. Introduction Aminoacyl-tRNA synthetases (ARSs) are indispensable enzymes that attach specific amino acids to their corresponding tRNAs, which is the fundamental step in translating the genetic code into proteins 1 – 4 . While their canonical function is conserved across species, ARSs exhibit striking variability in structure and oligomeric states 5 , implying their diverse and context-dependent roles beyond aminoacylation 6 . Over the course of evolution, many ARSs have acquired additional domains that enable interactions with other synthetases and cellular proteins, allowing them to participate in various regulatory and signaling pathways. These structural adaptations, along with the integration of scaffold proteins, have led to the formation of multi-tRNA synthetase complexes (MSCs) with species-specific compositions 6 – 8 . In humans, eight cytosolic ARSs (glutamyl-prolyl, isoleucyl, leucyl, methionyl, glutaminyl, arginyl, lysyl, and aspartyl tRNA synthetases) form MSC with three scaffold proteins known as aminoacyl-tRNA synthetase-interacting multifunctional proteins (AIMP1/p43, AIMP2/p38, and AIMP3/p18) 9 . The MSC functions as a central hub that orchestrates the non-canonical activities of its components 10 , including roles in cell signaling, apoptosis, immune modulation, and stress sensing. Recent comprehensive reviews have highlighted the involvement of various ARSs in tumorigenesis, emphasizing their potential as therapeutic targets and diagnostic markers 11 – 14 . Among MSC components, leucyl-tRNA synthetase 1 (LARS1) stands out as one of the most functionally significant, due to its role as a leucine sensor in the mammalian target of rapamycin complex 1 (mTORC1) signaling pathway 15 – 19 . Beyond its canonical function of charging tRNA Leu , LARS1 acts as a leucine-responsive GTPase-activating protein (GAP) that directly binds and activates Ras-related GTP-binding protein D (RagD) 20 . LARS1 also activates vacuolar protein sorting 34 (VPS34) in response to amino acid signals 21 , where both RagD and VPS34 promote mTORC1 activation - a key regulatory node for cell growth, metabolism, and autophagy 22 . As such, LARS1 has become a focal point in studies of nutrient sensing, metabolic disorders, and cancer biology, particularly due to the therapeutic relevance of mTORC1 signaling. Despite these findings, several critical gaps remain regarding the regulation of the non-canonical functions of LARS1 and, by extension, the components of MSC. First, while LARS1 is known to translocate to the mTORC1 on lysosomal surfaces upon leucine stimulation, the molecular mechanisms underlying this dynamic relocalization are poorly understood. In particular, it remains unclear whether this transition is initiated by specific events such as post-translational modifications, proteolytic cleavage, or other stimulus-dependent mechanisms. Second, it is still uncertain whether LARS1 must dissociate from the MSC to activate mTORC1. Finally, the high-resolution structural organization of LARS1 within the MSC remains unresolved, although IARS1 has been reported to be the linker of LARS1 to the MSC 23 , 24 . In this study, we determined the cryo-electron microscopy (cryo-EM) structure of the LARS1:IARS1 complex and investigated the regulatory mechanisms that govern LARS1-mediated non-canonical signaling within the MSC. Structural and mass spectrometry analysis revealed phosphorylation sites at the LARS1:IARS1 interface that may regulate their interaction. Further experiments revealed that these phosphorylation events are triggered by leucine stimulation and modulate LARS1’s association with the MSC, ultimately leading to mTOR1 activation. Our findings advance our understanding of how LARS1 transduces amino acid signals to mTOR1 and provide key insights into how the MSC dynamically mobilizes its components for context-dependent, non-canonical cellular functions. 2. Results and Discussion LARS1:IARS1 association is mediated by LARS1 UNL-L domain Since IARS1 is known to be the linker connecting LARS1 to the MSC complex 24,25 , we aimed to determine the LARS1:IARS1 complex structure to gain insight into the spatial organization of LARS1 within the MSC. To do this, human LARS1 and IARS1, both in full-length form, were co-expressed in E. coli BL21 (DE3) and purified using affinity and size exclusion chromatography. IARS1, which is unstable when expressed alone, becomes stabilized through complex formation with LARS1, thereby facilitating the purification of the complex. The cryo-EM structure of the LARS1:IARS1 complex was determined at 3.2 Å resolution (Fig. 1, Table 1, Supplementary Figs. 1 and 2). Although full-length proteins were used, the resulting density map resolved only partial regions of both components. For the IARS1, most catalytic region, including the connective peptide (CP), catalytic domain (CD), and anticodon-binding domain (ABD), was visualized, while only the UNE-L domain was observed in the LARS1. Most of the catalytic region of the LARS1 and the C-terminal junction and UNE-I domains of the IARS1 were not resolved, demonstrating the high mobility of these domains compared to the resolved region (Fig. 1a, b). During cryo-EM data processing, we identified a few 2D classes that display additional density to the visualized LARS1:IARS1 complex (Fig. 1c). We generated a 3D reconstruction using these 2D classes, and could observe the core module of LARS1 connected to the UNE-L domain, although the map resolution was low, hindering the detailed structural analysis of the complex (Fig. 1c). The Rag-binding domain (RBD) of LARS1 was positioned adjacent to the UNE-L domain in the map, however, it did not interact directly with IARS1, confirming that only UNE-L domain forms a stable interface with IARS1 as suggested by previous studies using size exclusion chromatography and XL-MS analysis 24,26 . The interface between LARS1 and IARS1 involves the CD and CP3 domains of IARS1 and the UNE-L domain of LARS1, forming a contact surface of approximately 1140 Å 2 , as analyzed by ePISA 27 (Supplementary Table 1). The interactions at this interface include hydrogen bonds, salt bridges, and hydrophobic contacts (Fig. 2a, Supplementary Tables 2, 3, and 4). Multiple sequence alignment (MSA) combined with structural analysis revealed that the key IARS1 interface residues are well-conserved among species whose LARS1s contain the UNE-L domain (Figs. 2b, c). In contrast, the corresponding residues, especially within the CP3 domain, are poorly conserved in the IARS1 of the invertebrates, which lacks the LARS1 UNE-L domain. This suggests that the LARS1:IARS1 interaction evolved after the emergence of vertebrates and has been conserved in species that harbor UNE-L domains in their LARS1. Furthermore, to gain insight into the dynamic behavior of interface residues and to identify functionally important residues not resolved in the cryo-EM structure, we performed molecular dynamics (MD) simulations (Supplementary Fig. 3). The simulation results were consistent with the cryo-EM analysis, showing that residues involved in hydrogen bonding, salt bridge formation, and the hydrophobic core in the structure significantly contribute to the binding enthalpy. Notably, the highly conserved residues in the IARS1 interface, Asn1076, Phe1081, and Arg1126, showed larger enthalpic contributions than other interfacial residues. This convergence of data from structural analysis, MD simulations, and multiple sequence alignment underscores the consistency and significance of these findings. The cryo-EM density map of the LARS1:IARS1 complex also includes the first high-resolution structure of human IARS1. Previously, the only known eukaryotic IARS1 structures were those from Candida albicans and Saccharomyces cerevisiae 28,29 . Despite species differences, these structures exhibit a conserved domain architecture with the human homolog, comprising the CD, which facilitates aminoacylation of tRNA Ile , the CP domain, which mediates interdomain flexibility and serves as a platform for protein–protein interactions, and the ABD, responsible for recognizing and stabilizing the tRNA anti-codon loop (Supplementary Fig. S4). Structural comparisons yielded low RMSD(Cα) values of 1.337 Å (with C. albicans , PDB: 6LDK) and 1.879 Å (with S. cerevisiae , PDB: 8WND), indicating strong conservation across species. Amino acid stimulation triggers dissociation of LARS1 from the MSC It remains unclear whether LARS1 must dissociate from the MSC to exert its noncanonical function, such as mTORC1 activation. Since IARS1 is the binding partner anchoring LARS1 to the MSC 25 , we investigated whether LARS1’s role in amino acid-dependent mTORC1 activation requires the dissociation of the LARS1:IARS1 complex. Previous studies reported that LARS1, but not IARS1, colocalizes with mTOR and Raptor upon amino acid stimulation, suggesting that its dissociation from IARS1 may underlie relocalization and signaling activation 15 . To directly test whether amino acid stimulation affects LARS1:IARS1 interaction, we performed time-course analyses assessing both downstream pathway activation and complex integrity (Figs. 3a, b). HEK293T cells overexpressing FLAG-tagged LARS1 were subjected to amino acid starvation for 24 hours. The medium was then replaced with one containing complete amino acids, and the cells were harvested at different time points for analysis. The mTORC1 activation was measured by the phosphorylation of Thr389 on ribosomal protein S6 kinase beta-1 (S6K1), a well-established downstream target of mTORC1. The mTORC1 activity showed a sharp increase within the first hour and remained elevated throughout the 4-hour observation period (Fig. 3a). In parallel, immunoprecipitation (IP) of LARS1 followed by western blot analysis revealed a rapid decline in LARS1:IARS association within the first hour after amino acid stimulation, which was sustained at low levels for the remainder of the observation period (Fig. 3b). The temporal coincidence and parallel patterns of mTORC1 activation and LARS1 dissociation suggest that LARS1 liberation from the MSC may be functionally associated with activation of the mTORC1 signaling pathway. Amino acid stimulation induces phosphorylation of the LARS1 UNE-L domain LARS1 is known to undergo a conformational change that facilitates its interaction with RagD upon binding of leucine 20 . Having established that LARS1 dissociates from IARS1 upon an amino acid stimulus, we next investigated whether this dissociation results from direct amino acid binding. To test this, we co-expressed human LARS1 with GST-tagged IARS1 in E. coli BL21 (DE3), purified the complex, and incubated it with either an excess amount of leucine, isoleucine, or ATP (Supplementary Fig. 5). Subsequent glutathione resin pull-down assays showed no significant difference in the amount of LARS1 co-precipitating with IARS1 under these conditions, implying that additional layer of cellular signaling is required to trigger LARS1 dissociation, as observed in the cell-based assays (Figs. 3a, b). As potential signals triggering LARS1 dissociation from the MSC, proteolytic cleavage and specific post-translational modifications (PTMs) have been shown to regulate the subcellular localization and functional repertoire of MSC components 13 . Several MSC components, including AIMP1 30 , QARS 31 , KARS 32 , and EPRS 33 , undergo protease-mediated cleavage that facilitates their release from the complex and subsequent engagement in non-canonical functions. Phosphorylation has also been implicated in modulating the noncanonical roles of MSC components such as EPRS 34 , KARS 35 , MARS 36 , and IARS1 37 . To determine whether LARS1 is subject to similar regulation, HEK293T cells expressing FLAG-tagged LARS1 were subjected to amino acid starvation for 24 hours, followed by stimulation with either 2 mM leucine or a complete amino acid mixture (Supplementary Fig. 6). Western blot analysis confirmed increased mTORC1 activity following both leucine and total amino acid stimulation. Notably, SDS-PAGE analysis of the immunoprecipitated FLAG-LARS1 showed no gel band shift before or after amino acid stimulation, thereby ruling out the possibility of proteolytic cleavage as a regulatory mechanism. We next conducted mass spectrometry (MS) analysis on immunoprecipitated LARS1 to identify PTMs associated with amino acid stimulation. The analysis revealed distinct phosphorylation patterns depending on the nutritional conditions (Figure 3c, Supplementary Fig. 7). Specific phosphorylation sites were detected exclusively under amino acid starvation, while others appeared only upon amino acid stimulation. For example, phosphorylation at Ser720, located within a KMSKS motif and previously reported as a target of Unc-51-like autophagy-activating kinase 1 (ULK1) 38 , was detected specifically under amino acid starvation but was absent following amino acid stimulation. Notably, amino acid stimulation triggered multiple phosphorylation events within the UNE-L domain (residues 1064-1176), particularly at the LARS1:IARS1 interface (Figs. 2a, Supplementary Fig.7). MS/MS spectra identified a peptide triply phosphorylated at Ser1070, Ser1077, and Ser1082, as confirmed by four independent peptide spectrum matches (PSMs) (Figure 3c). Phosphorylation at Ser1068 was also detected, but at a lower frequency, indicating it is a minor site. These results suggest that the clustered phosphorylation sites on the UNE-L domain function as a potential regulatory hotspot governing the LARS1’s association with IARS1, presumably in the MSC. Phosphorylation of the LARS1 UNE-L domain disrupts the LARS1:IARS1 complex We then conducted both in vitro and cellular experiments to assess how phosphorylation of the UNE-L domain affects the integrity of the LARS1:IARS1 complex. To precisely control the phosphorylation state at specific serine residues, we employed a genetic code expansion technique 39,40 , which enables site-specific incorporation of O -phosphoserine during protein synthesis. This system utilizes an orthogonal translation machinery consisting of phosphoseryl-tRNA synthetase (SepRS), an amber suppressor tRNA (tRNA Sep ), and an engineered elongation factor for phosphoseryl-tRNA (EF-Sep). This approach allows for precise phosphorylation at a targeted site without requiring prior knowledge of the responsible kinase. Since UNE-L is the only LARS1 domain involved in complex formation with IARS1, we produced C-terminal His 6 -tagged UNE-L (LARS1 residues 1064-1176) with phosphorylation at Ser1070, Ser1077, or Ser1082 (Fig. 4a, Supplementary Fig. 8). Ser1068 was excluded because it is located relatively distant from the LARS1:IARS1 interface compared to the other sites. The phosphorylation status of the produced proteins was verified by mass spectrometry (Supplementary Figs. 9, 10). To assess the effect of phosphorylation on IARS1 binding, we conducted pull-down assays using GST-IARS1 fusion protein and UNE-L proteins with different phosphorylation states (Fig. 4b). The UNE-L variants were mixed with GST-IARS1 and incubated on ice for 1 hour, followed by GST pull-down assays. Wild-type UNE-L was efficiently pulled down with GST-IARS1, as expected. In contrast, the phosphorylated UNE-L variants (Ser1070Sep, Ser1077Sep, and Ser1082Sep) showed significantly reduced binding. Furthermore, when phosphorylated LARS1 UNE-L variants were dephosphorylated by lambda phosphatase at 30°C for 30 minutes, the binding defect was reversed. This indicates that phosphorylation at these sites is sufficient to disrupt LARS1:IARS1 complex formation in vitro . To further investigate the impact of phosphorylation at the LARS1 UNE-L domain in a cellular context, FLAG-tagged full-length wild-type LARS1 or various phosphomimetic variants (Ser1070Glu, Ser1077Glu, Ser1082Glu, Ser1077/1082Glu, and Ser1070/1077/1082Glu) were transfected into HEK293T cells. A co-immunoprecipitation (co-IP) assay was then performed using an anti-FLAG antibody to pull down FLAG-LARS1 and detect associated IARS1. Consistent with the in vitro findings, only the wild-type LARS1 co-immunoprecipitated with IARS1, while the phosphomimetic variant, whether single-site, double-site, or triple-site, failed to pull down IARS1 (Fig. 4c). Combined with the mass spectrometry data, these results demonstrate that phosphorylation of the LARS1 UNE-L domain at residues Ser1070, Ser1077, and Ser1082, triggered by amino acid stimulation, is responsible for the dissociation of LARS1 from IARS1, and ultimately from the MSC. Phosphomimetic mutants of the LARS1 UNE-L domain promote mTORC1 activation Having established the primary role of this phosphorylation in LARS1, we next examined whether phosphorylation-induced dissociation affects LARS1’s function in activating mTORC1 (Supplementary Fig. 11). Exogenous expression of wild-type LARS1 led to a modest increase in mTORC1 activity compared to the empty vector control, indicating that elevated cytosolic LARS1 induces only a marginal mTORC1 activation. In contrast, single phosphomimetic variants, Ser1077Glu and Ser1082Glu, triggered a pronounced increase in mTORC1 activity, up to threefold over empty vector control. The elevated activity observed with the Ser1082Glu variant was reduced when the phosphomimetic residue was substituted with alanine, supporting that phosphorylation at Ser1082 enhances mTORC1 activation beyond merely disrupting hydrogen bonding at the interface (Supplementary Fig. 11). Interestingly, although the single, double, and triple phosphomimetic variants all showed similar dissociation patterns from IARS1 in the co-IP assay, their effects on mTORC1 activation differed markedly (Fig. 4d). In particular, Ser1070Glu exhibited activity comparable to wild-type LARS1, whereas Ser1077Glu and Ser1082Glu exhibited robust activation. Of particular note, the triple mutant Ser1070/1077/1082Glu, which mimics the phosphorylation pattern observed upon amino acid stimulation, induced the highest level of mTORC1 activation, approximately 3.3-fold greater than wild-type LARS1. These results demonstrate that mTORC1 activation is not simply driven by the release of LARS1 from the MSC, but is critically modulated by specific phosphorylation events on LARS1. This is also consistent with the result that the UNE-L-deficient LARS1 showed reduced mTORC1 activation compared to the Ser1077Glu and Ser1082Glu mutants (Supplementary Fig. 11). Therefore, coordinated phosphorylation of the UNE-L domain likely functions as a key regulatory switch that not only promotes LARS1 dissociation from the MSC but also enhances its capacity to activate mTORC1, potentially via specific interactions with upstream effectors of the mTORC1 pathway. ARS complex structures provide insights into the overall architecture of the MSC The complexation of ARSs into the MSC provides a functional platform for individual synthetases to carry out noncanonical roles in response to external stimuli. Although biochemical, crosslinking mass spectrometry, and molecular docking studies have provided insights into the three-dimensional organization of the human MSC 26 , its complete high-resolution structure remains unresolved. The human MSC is known to include eight ARS (EPRS1, IARS1, LARS1, MARS1, QARS1, RARS1, KARS1, and DARS1) and three scaffold proteins (AIMP1:p43, AIMP2:p38, AIMP3:18), organized into three subcomplexes 41 . Subcomplex I includes QARS1, RARS1, KARS1, AIMP1, and AIMP2; Subcomplex II contains MARS1, AIMP3, EPRS1, and AIMP2; Subcomplex III comprises EPRS1, IARS1, and LARS1. Recent high-resolution structural studies have provided critical insights into the organization of these subcomplexes. In Subcomplex I, KARS1 forms a dimer and interacts with the N-terminal region of AIMP2. In Subcomplex II, the MARS1 GST :AIMP3 GST :EPRS GST :AIMP2 GST complex structure reveals a core architecture formed by GST-like domains, while the DARS1:AIMP2 GST :EPRS GST complex structure further details its assembly 42,43 . In Subcomplex III, structural inshgits have emerged from both the crystal structures of the EPRS1:IARS1 complex and our cryo-EM structure of the LARS1-IARS1 complex. In the EPRS1:IARS1 complex, the EARS1 ABD interacts with the UNE-I domain of IARS1, with a hairpin loop from the ABD inserting into the cleft between UBX1 and UBX2 domains of the UNE-I 37 . Phosphomimetic mutations at two serine residues within this loop disrupt the interaction, suggesting that phosphorylation at these sites promotes the release of IARS1 from the MSC, a mechanism that parallels our findings for LARS1. Taken together, these structural findings, including our LARS1:IARS1 complex structure, enable the construction of a molecular model for MSC, and especially for Subcomplex III. While individual synthetases likely retain conformational flexibility, the components are interconnected through robust, domain-mediated interactions: UNE-L links LARS1 to IARS1, and UNE-I connects IARS1 to EPRS1 (Supplementary Fig. 12). 3. Discussion Based on our structural, biochemical, and cellular findings, we propose a comprehensive model for LARS1 regulation and its role in amino acid sensing and mTORC1 activation (Fig. 5 ). Under amino acid starvation, a significant portion of LARS1 is sequestered within the MSC via interaction with IARS1, limiting its availability for non-canonical signaling functions. Upon amino acid stimulation, as yet unidentified nutrient-responsive kinases trigger multi-site phosphorylation of the LARS1 UNE-L domain. This phosphorylation event destabilizes the LARS1–IARS1 interaction, shifting the equilibrium toward LARS1 dissociation from the MSC. Free cytosolic LARS1, when bound to leucine, has been reported to exhibit increased affinity for RagD:GTP and translocate to the lysosomal membrane 15 , 20 . There, it activates mTORC1 either by promoting the hydrolysis of RagD:GTP to RagD:GDP 20 or by activating VPS34, which has been shown to directly interact with the UNE-L domain of LARS1 21 . Given the critical role of UNE-L domain phosphorylation in mTORC1 activation, this modification likely acts as a key regulator that promotes LARS1's interaction with mTORC1-activating partners. This study highlights phosphorylation as a critical molecular switch that regulates LARS1's non-canonical functions in mTORC1 activation, exemplifying how the MSC efficiently responds to external stimuli. Phosphorylation has also been observed in other MSC components, including MARS 36 , KARS 35 , 44 – 53 , EPRS 34 , 54 – 60 , and IARS1 37,61 , suggesting a conserved regulatory mechanism within the complex. This mechanism involves rapid activation of specific kinase(s) by an external cue, leading to the prompt dissociation of selected MSC components and subsequent activation of downstream signaling pathways. These observations contribute to a broader understanding of the MSC as a dynamic and modular signaling platform that integrates structural plasticity with stimulus-responsiveness, rather than functioning as a rigid, static megacomplex. This inherent structural flexibility may account for why elucidating the whole structure of the MSC has remained elusive. The present study is expected to lead to the identification of key nutrient-responsive kinase(s) responsible for phosphorylating critical sites in the LARS1-UNE-L domain, thereby activating mTORC1 signaling. These findings may ultimately guide the development of novel therapeutic strategies for cancer and metabolic disorders involving dysregulated mTORC1 activity. 4. Methods Plasmid construction and protein purification LARS1:IARS1 Complex The full-length human LARS1 gene, without any affinity tag, was inserted into a pCDF-Duet vector between the NcoI and AscI restriction sites. The full-length human IARS1 gene was cloned as an N-terminal hexahistidine (His 6 )-glutathione S-transferase (GST) fusion with a Tobacco etch virus (TEV) protease cleavage site into the pET28a vector’s NcoI and XhoI restriction sites. Both recombinant genes were cloned using Gibson assembly (NEB). Escherichia coli BL21(DE3) cells were sequentially transformed with both plasmids to co-express the LARS1:IARS1 complex. Transformed cells were cultured in 6.4 L of LB medium at 30°C until the optical density at 600 nm reached 0.6. Protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG). After induction, the temperature was lowered to 23°C, and cells were grown for an additional 18 hours before harvest. Cells were lysed by sonication in lysis buffer (20 mM Tris-HCl pH 8.0, 200 mM NaCl, 10 mM imidazole, 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM dithiothreitol (DTT)) supplemented with protease inhibitor cocktail (Roche). The cell lysate was clarified by centrifugation, and the supernatant was incubated with Ni-NTA agarose resin (Qiagen). The resin was washed with wash buffer (20 mM Tris-HCl pH 8.0, 200 mM NaCl, 40 mM imidazole, 1 mM DTT), and the bound proteins were eluted with elution buffer (20 mM Tris-HCl pH 8.0, 200 mM NaCl, 250 mM imidazole, 1 mM DTT). The eluted proteins were incubated with TEV protease at a 1:100 (w/w) ratio and dialyzed against dialysis buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 14 mM β-mercaptoethanol) to remove the His 6 -GST tag. After cleavage, the protein solution was concentrated using a 100 kDa cut-off ultracentrifugal filter (Amicon). The LARS1:IARS1 complex was isolated by size exclusion chromatography using a Superdex™ 200 increase 10/300 GL column (Cytiva) equilibrated with SEC buffer (20 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM DTT). Peak fractions containing the LARS1:IARS1 complex were collected and concentrated using a 100 kDa ultracentrifugal filter (Amicon) for cryo-EM grid preparation. Phospho-UNE-L domain To produce wild-type UNE-L protein (UNE-L-WT), the plasmid pCDF-Duet-UNE-L-WT was constructed by cloning the gene for human LARS1 UNE-L domain (residues 1064–1176) with an N-terminal His 6 -tag between the NcoI and AscI sites of pCDF-Duet (Novagen). For the production of UNE-L domains with a phosphoserine (Sep) at specific positions, phosphoserine variants Ser1070Sep, Ser1077Sep, and Ser1082Sep were generated by introducing amber stop codons (TAG) at the corresponding positions via overlap PCR. The resulting PCR fragments were cloned into the pCDF-Duet SepT vector 40 . For the expression of phosphoserine-containing UNE-L variants, the corresponding plasmids were transformed into E. coli BL21(DE3) cells carrying the advanced Sep incorporation system (pKD-SepRS9-EFSep21) 39 , 40 . Transformed cells were grown in 2xYT medium at 30°C until the optical density at 600 nm reached 0.8. The temperature was then lowered to 23°C, and protein expression was induced by adding 0.5 mM IPTG and incubating for 24 hours. Phosphoserine-containing UNE-L variants and wild-type UNE-L were purified by Ni-NTA agarose column chromatography. The eluted proteins were concentrated using 3 kDa cutoff ultracentrifugal filters (Amicon). GST-IARS1 For GST-IARS1 protein purification, the sequence was cloned into a pET-21a vector using NcoI-AscI restriction sites. The recombinant plasmid was transformed into E. coli BL21(DE3). Cells were grown in 6.4 L of LB medium at 37°C until the optical density at 600 nm reached 0.6. Then, protein expression was induced with 0.5 mM IPTG at 18°C for 18 hours. Cells were harvested by centrifugation, resuspended in phosphate-buffered saline (PBS), and lysed by sonication. After centrifugation, the clear supernatant was incubated with glutathione sepharose resin (GenScript) for 1 hour at 4°C. The resin was washed with PBS (10 column volumes × 5 times), and the protein was eluted with 33 mM glutathione in 50 mM Tris-HCl buffer (pH 8.0). Cryo-EM sample preparation The purified LARS1:IARS1 complex at a concentration of 0.5-1 mg/mL was applied to C-Flat 1.2/1.3 3 Au holey carbon grids (Electron Microscopy Sciences) that had been glow-discharged for 40 seconds. A 3.5 µL aliquot of the sample was applied to the grid, blotted for 5 seconds at 4°C with 100% humidity using a Vitrobot Mark IV (Thermo Fisher Scientific), and plunge-frozen in liquid ethane. Cryo-EM data acquisition Data was collected on a 300kV Titan Krios G2 electron microscope (Thermo Fisher Scientific) at the Pacific Northwest Center for Cryo-EM (PNCC). Micrographs were recorded using a Falcon 4 direct electron detector operating in electron counting mode. All data acquisition was performed automatically using EPU software (Thermo Fisher Scientific), with micrographs saved in the electron-event representation (EER) format. Micrographs were collected at a calibrated pixel size of 0.814 Å with a defocus range varying from − 0.8 µm to -2.5 µm at a total electron dose of 60 e − /Å for 3.8 seconds. A total of 7,504 micrographs were acquired for subsequent processing. Cryo-EM data processing All data processing was performed using cryoSPARC 4.2 62 . Movie frames were motion-corrected using patch motion correction in cryoSPARC. The contrast transfer function (CTF) parameters were estimated using patch CTF estimation in cryoSPARC. Particles were automatically picked using the blob picker in cryoSPARC, yielding approximately 4.69 million particles. The extracted particles were subjected to multiple rounds of 2D classification in cryoSPARC, resulting in 947,600 selected particles. Initial 3D model generation was performed using ab initio reconstruction (n = 4) in cryoSPARC, which yielded a model consistent with the expected LARS1:IARS1 complex. The particles were further classified using heterogeneous refinement with four classes (n = 4), using the same ab initio map as the reference. Among these classes, 135,800 particles were selected and subjected to homogeneous refinement, and further to non-uniform refinement, yielding a final resolution of 3.16 Å. Model building and refinement For initial model building, AlphaFold3 webserver was used to predict the structures of human IARS1 (residues 1-800) and the human LARS1 UNE-L domain (residues 1067–1176) based on their respective amino acid sequences 63 . These predicted models were fitted into the cryo-EM density map using UCSF Chimera (version 1.11.12) 64 . The initial models were then docked into the density map using the Dock_in_map utility in PHENIX (version 1.18.2) 65 . The model was iteratively refined by PHENIX real-space refinement and manual adjustment in Coot (version 0.8.9) 66 . The final model was validated using the MolProbity web server 67 . The figures were made using UCSF ChimeraX (version 1.9) 68 . GST Pull-down assay The mixture of GST-IARS1 and UNE-L domain samples, either treated with or without lambda phosphatase, was incubated with glutathione sepharose resin (GenScript) at 4°C for 2 hours. The resin was washed with 10 column volumes of PBS five times, and the GST-tagged protein was eluted with 33 mM glutathione in 50 mM Tris-HCl buffer (pH 8.0). Mammalian cell culture and treatment LARS1 and its mutants were cloned into a pcDNA3.1 vector with an N-terminal FLAG epitope tag (DYKDDDDK) for expression in mammalian cells. HEK293T cells were transfected with each plasmid using AccuFect™ reagent (Bioneer), following the manufacturer’s instructions. Wild-type and transgenic HEK293T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37°C with 5% CO 2 . For amino acid starvation, cells were washed twice and incubated in amino acid-free DMEM for 24 hours. Amino acid stimulation was performed by adding DMEM containing total amino acids for the indicated time. Cell lysis, immunoprecipitation, and immunoblot assays Transfected HEK293T cells were washed with cold PBS and lysed in RIPA buffer containing benzonase (Enzynomics), protease inhibitor cocktail (Roche), and phosphatase inhibitor cocktail (Roche) at 4°C for 3 hours. Cell lysates were centrifuged at 13,000 rpm for 30 min at 4°C to obtain clear supernatants. Immunoprecipitation for FLAG-LARS1 was performed by incubating the cleared supernatants with anti-DYKDDDDDK G1 affinity resin (GenScript) at 4°C for 2 hours under agitation. The beads were then washed three times with PBS containing 0.1% NP-40. The washed agarose beads were either loaded directly onto an SDS-PAGE gel or subjected to additional purification. Proteins were eluted using PBS supplemented with 5 mM 3×FLAG peptide (MedChemExpress). The eluted protein complexes were analyzed by SDS-PAGE and then subjected to Western blotting or mass spectrometry analysis. Proteins were transferred to PVDF membranes using a semi-dry transfer method with the Trans-Blot Turbo Transfer System (Bio-Rad) at 20 V for 1 hour. Proteins transferred were probed with corresponding antibodies, and immunoreactive bands were visualized using chemiluminescence. The antibodies used for immunoblot analysis included the following: P-S6K1 (Cell Signaling Technology, #9205S), total S6K1 (Cell Signaling Technology, #9202S), FLAG (Cell Signaling Technology, #8146S), GAPDH (Cell Signaling Technology, #2118S), and IARS11 (Abcam, ab31533). Immunoblot quantification was performed by measuring relative band intensities using ImageJ software 69 , with normalization as indicated. MALDI-MS/MS analysis Proteins pulled down with the anti-FLAG resin were subjected to SDS-PAGE. Following electrophoresis, corresponding bands were immediately excised using a clean scalpel. The gel slices were transferred into 1.5 mL low-peptide-binding microtubes and processed for destaining and dehydration as previously described 70 . A sufficient amount of trypsin solution was added to cover the dried gel. The samples were placed on ice to facilitate gel immersion, with vortexing and brief centrifugation every 10 min. After 60 min, 50 mM ammonium bicarbonate solution was added until the gel became transparent. The samples were incubated overnight at 37°C for tryptic digestion. Following digestion, 1 µL of peptide extract was spotted onto 1 µL of pre-crystallized matrix on a 384-well-polished stainless-steel target (Bruker Daltonics). The analyses were performed using either α-cyano-4-hydroxycinnamic acid (10 mg/mL), sinapinic acid (10 mg/mL), or 2,5-dihydroxybenzoic acid (20 mg/mL) matrices. The spotted mixtures were allowed to air dry at room temperature. Mass spectrometry analysis was performed using a Bruker Autoflex Speed TOF/TOF mass spectrometer (Bruker Daltonics) at KAIST Analysis Center for Research Advancement (KARA). Mass spectra were obtained in positive-ion detection mode with reflectors, and each mass spectrum was generated by superimposing ten sets of 2,000 laser scans. For MALDI-TOF/TOF MS/MS analysis, the Laser Ionization Fragmentation Technology (LIFT) mode was used, with an initial accelerating voltage of 6 kV and a subsequent accelerating voltage of 19 kV for the LIFT cell. The detection frequency was set to 200 Hz. Each mass spectrum was derived from three superimpositions of 500 laser scans each. The acquired mass spectra were processed using FlexAnalysis software (version 3.3.65.0) (Bruker Daltonics). LC-MS/MS FLAG-tagged LARS1 protein was immunoprecipitated from HEK293T cell lysates using anti-FLAG agarose resin. The resin was washed three times with PBS (10 column volumes each). Following washing, the resin was mixed with 6x SDS-PAGE loading dye (LPS Solutions) and heated at 95°C for 10 minutes to elute the proteins. After cooling, the samples were subjected to SDS-PAGE separation. Protein bands corresponding to the expected molecular weight of LARS1 were excised for in-gel digestion 70 . Following tryptic digestion, peptides were desalted using Graphite Spin Columns (Thermo Fisher Scientific) according to the manufacturer's protocol. The desalted peptides were resuspended in 0.1% trifluoroacetic acid (TFA) and stored at -20°C until LC-MS/MS analysis. LC-MS/MS analysis was performed using a Vanquish™ Neo UHPLC System coupled with an Orbitrap Eclipse™ Tribrid™ Mass Spectrometer (Thermo Fisher Scientific) at KARA. Peptides were first loaded onto an Acclaim™ PepMap™ 100 C18 LC Trap Column (75 µm × 2 cm, 3 µm, 100 Å) and then separated on a Double nanoViper™ PepMap™ Neo Analytical Column (2 µm, C18, 75 µm × 500 mm, 1500 bar) (Thermo Fisher Scientific). The mobile phases consisted of 0.1% formic acid in water (solvent A) and 0.1% formic acid in 80% acetonitrile (solvent B). Peptides were eluted using a linear gradient from 5–40% solvent B over 80 minutes at a flow rate of 300 nL/min. MS data were acquired in positive ion mode with a resolution of 240,000 at m/z = 200. For data-dependent acquisition (DDA), the instrument operated in Top 3-second cycle mode. Full MS scans were acquired over a mass range of m/z 375–1500 with an automatic gain control (AGC) target value of 250% and a maximum injection time was set to auto. Precursor ions exceeding an intensity threshold of 2.0e5 were selected for fragmentation. Fragmentation was performed using higher-energy collisional dissociation (HCD) with a normalized collision energy of 30%. MS/MS spectra were acquired with an AGC target of 5.0 × 10 4 , a maximum injection time set to auto, and an isolation window of 1.6 m/z. Dynamic exclusion was set to 60 seconds to minimize repeated selection of precursors. Raw data files were processed using Proteome Discoverer 3.1 (Thermo Fisher Scientific). Database searches were performed against the UniProt Homo sapiens database (Proteome ID. UP000005640) and human LARS1 sequence (Uniprot Entry: Q9P2J5) using trypsin as the digestion enzyme. Post-translational modifications were searched using Sequest HT node (precursor tolerance: 0.1 Da, fragment tolerance: 0.05 Da). Static modifications included carbamidomethylation of cysteine (+ 57.021 Da). Dynamic modifications included oxidation of methionine (+ 15.995 Da), acetylation of protein N-termini (+ 42.011 Da), and phosphorylation of serine, threonine, and tyrosine residues (+ 79.966 Da) for phosphorylation studies Declarations Acknowledgements We thank Sung Gyu Lee (KARA) and Marcelo de Farias (PNCC) for microscope support, and Sujeong Kwon (KARA) for assistance with the Orbitrap Eclipse Tribrid MS analysis. Supplementary data Supplementary Data Conflict of Interest None declared. Funding This work was supported by the National Research Foundation of Korea (2023R1A2C1005945 to H.S.P., RS-2024-00344154 to J.Y.K) and PNCC (#160183). Data availability The Cryo-EM map of the LARS1:IARS1 complex has been deposited in the Electron Microscopy DataBank (EMDB) under accession numbers EMD-65488, and the coordinates have been deposited in the RCSB Protein DataBank under accession code 9W01. They are publicly available as of the date of publication. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7316280","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":507583074,"identity":"5aea0e19-6090-4885-b8a3-57d36a662f12","order_by":0,"name":"Hee-Sung Park","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA1ElEQVRIiWNgGAWjYBACAzB5wMaAgRkqIkGkljSQFsYGUrQcBtFEajHnP3vwc8WZ88b87ezPHzDU2DFIzj6AX4vljLxkyTM3bptJHOYxbGA4lswgzZdAwGE3eAwkGz7ctmE4zAN0GNsBBjkeQn45f8b4Z8OHczbyh9kfNjD8I0bLgRwzyYYbB8wMDjMYNjC2HWCQJqjlRl6aZcOZZGNDoF9mJPYl80j2EHTY2cM3G47ZGc47f/zBhw/f7OQkzhDQwsCA7I4EVC4xWkbBKBgFo2AUYAMAmFNCz0nWJ1gAAAAASUVORK5CYII=","orcid":"https://orcid.org/0000-0001-8645-0405","institution":"Korea Advanced Institute of Science and Technology","correspondingAuthor":true,"prefix":"","firstName":"Hee-Sung","middleName":"","lastName":"Park","suffix":""},{"id":507583075,"identity":"79851cf5-663f-4c81-8cf2-51d4f1462e77","order_by":1,"name":"Youjin Kim","email":"","orcid":"","institution":"Korea Advanced Institute of Science and Technology","correspondingAuthor":false,"prefix":"","firstName":"Youjin","middleName":"","lastName":"Kim","suffix":""},{"id":507583076,"identity":"a76e37e4-8a26-4b49-a4fc-10cc9e411a84","order_by":2,"name":"Joo-Chan Kim","email":"","orcid":"https://orcid.org/0000-0001-8758-4085","institution":"Korea Advanced Institute of Science and Technology","correspondingAuthor":false,"prefix":"","firstName":"Joo-Chan","middleName":"","lastName":"Kim","suffix":""},{"id":507583077,"identity":"bb99da72-ef7d-404e-b9f1-3383b647569e","order_by":3,"name":"Do-Wook Kim","email":"","orcid":"","institution":"Korea Advanced Institute of Science and Technology","correspondingAuthor":false,"prefix":"","firstName":"Do-Wook","middleName":"","lastName":"Kim","suffix":""},{"id":507583078,"identity":"6674691d-94c0-4864-9594-83f8a61f9ee5","order_by":4,"name":"Jinwoo Kim","email":"","orcid":"https://orcid.org/0000-0002-7205-9170","institution":"KAIST","correspondingAuthor":false,"prefix":"","firstName":"Jinwoo","middleName":"","lastName":"Kim","suffix":""},{"id":507583079,"identity":"b2a78e02-840f-4b43-943f-48ca7a5ede1a","order_by":5,"name":"Jimin Lee","email":"","orcid":"","institution":"Korea Advanced Institute of Science and Technology","correspondingAuthor":false,"prefix":"","firstName":"Jimin","middleName":"","lastName":"Lee","suffix":""},{"id":507583080,"identity":"edce080f-05e0-4a86-8289-36435cb68e43","order_by":6,"name":"Sunghoon Kim","email":"","orcid":"https://orcid.org/0000-0002-1570-3230","institution":"Yonsei University","correspondingAuthor":false,"prefix":"","firstName":"Sunghoon","middleName":"","lastName":"Kim","suffix":""},{"id":507583081,"identity":"eeb07911-1de9-46bd-9186-da38876b2a0b","order_by":7,"name":"Jin Young Kang","email":"","orcid":"https://orcid.org/0000-0002-8493-7890","institution":"KAIST","correspondingAuthor":false,"prefix":"","firstName":"Jin","middleName":"Young","lastName":"Kang","suffix":""}],"badges":[],"createdAt":"2025-08-07 08:15:32","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7316280/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7316280/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":90316530,"identity":"cd1ce89b-79c3-4261-8960-c6309b7a0050","added_by":"auto","created_at":"2025-09-01 10:21:54","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":324160,"visible":true,"origin":"","legend":"\u003cp\u003eCryo-EM structure of human LARS1:IARS1 complex. (\u003cstrong\u003ea\u003c/strong\u003e) Domain organization of human LARS1 and IARS1 proteins. Key domains include: CD (Rossmann-fold catalytic domain), CP (connective peptide), SC-fold (stem contact fold), ABD (anticodon-binding domain), and UNE-L/UNE-I (UNE domains). Numbers indicate amino acid positions. Domains not resolved in the cryo-EM map are indicated with dotted white boxes. (\u003cstrong\u003eb\u003c/strong\u003e) Cryo-EM density map of the LARS1:IARS1 complex at 3.2 Å resolution, shown in two orientations (180° rotation). The UNE-L domain of LARS1 (purple) forms a specific interaction with IARS1. Domain colors correspond to those in Fig. 1a. (\u003cstrong\u003ec\u003c/strong\u003e) A 2D class average and a low-resolution cryo-EM density map showing the overall architecture of the complex, including the LARS1 Rag-binding domain.\u003c/p\u003e","description":"","filename":"1.png","url":"https://assets-eu.researchsquare.com/files/rs-7316280/v1/03bf9a90b56fc6e97dcec408.png"},{"id":90313189,"identity":"36ae18da-3ec5-4586-94d9-d4cb5fb2d1b7","added_by":"auto","created_at":"2025-09-01 10:05:54","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":391391,"visible":true,"origin":"","legend":"\u003cp\u003eStructural basis and species conservation of the LARS1:IARS1 binding interface. (\u003cstrong\u003ea\u003c/strong\u003e) The LARS1:IARS1 interface. Left: Overall structure of the LARS1:IARS1 complex with the interface region highlighted by red dashed lines. Right: Four enlarged views showing atomic-level interactions at the interface. Red and yellow dashed lines indicate salt bridges and hydrogen bonds, respectively. Atom types in brackets specify: [O] backbone carbonyl oxygen, [N] backbone amide nitrogen, [OG] serine side chain hydroxyl oxygen, [OD1]/[OD2] aspartic acid side chain carboxyl oxygens, [OE1]/[OE2] glutamic acid side chain carboxyl oxygens, and [NZ] lysine side chain amino nitrogen. (\u003cstrong\u003eb\u003c/strong\u003e) Multiple sequence alignments of IARS1-CD/CP3 and LARS1-UNE-L domains across nine species, ranging from Homo sapiens to Escherichia coli, were obtained using Mafft and visualized with JalView. Blue highlighting indicates evolutionarily conserved residues. Red bar = α-helix. Blue bar = β sheet. Black bars = loop. IARS1 protein sequence obtained from Uniprot. Uniprot Entry P41252; \u003cem\u003eHomo sapiens\u003c/em\u003e, Q8BU30; \u003cem\u003eMus musculus\u003c/em\u003e, Q8MSW0; \u003cem\u003eDrosophila melanogaster\u003c/em\u003e, Q21926; \u003cem\u003eCaenorhabditis elegans\u003c/em\u003e, P09436; Saccharomyces cerevisiae, P26499; \u003cem\u003eMethanothermobacter marburgensis\u003c/em\u003e, P56690; \u003cem\u003eThermus thermophilus\u003c/em\u003e, Q0IDA5; \u003cem\u003eSynechococcus sp.\u003c/em\u003e, P00956; \u003cem\u003eEscherichia coli\u003c/em\u003e. LARS1 protein sequence obtained from Uniprot. Uniprot Entry Q9P2J5; \u003cem\u003eHomo sapiens\u003c/em\u003e, Q8BMJ2; \u003cem\u003eMus musculus\u003c/em\u003e, Q8MRF8; \u003cem\u003eDrosophila melanogaster\u003c/em\u003e, Q09996; \u003cem\u003eCaenorhabditis elegans\u003c/em\u003e, P26637; \u003cem\u003eSaccharomyces cerevisiae\u003c/em\u003e, D9PU05; \u003cem\u003eMethanothermobacter marburgensis\u003c/em\u003e, Q5SLY2; \u003cem\u003eThermus thermophilus\u003c/em\u003e, Q0IAE0; \u003cem\u003eSynechococcus sp.\u003c/em\u003e, Q0TK31; \u003cem\u003eEscherichia coli\u003c/em\u003e. (\u003cstrong\u003ec\u003c/strong\u003e) ConSurf web server\u003csup\u003e72\u003c/sup\u003e analysis showing evolutionary conservation mapped onto the human IARS1 surface using different multiple sequence alignments (MSAs). Human IARS1 was used as the query sequence for comparative analysis. Left: Conservation analysis based on MSA of species that possess UNE-L domains. Center: IARS1 surface structure with the UNE-L interaction interface highlighted in red. Right: Conservation analysis based on MSA of species lacking UNE-L domains. Black dashed lines delineate the interface boundaries across all panels. The analysis reveals that the UNE-L binding interface is highly conserved among species that possess UNE-L domains.\u003c/p\u003e","description":"","filename":"2.png","url":"https://assets-eu.researchsquare.com/files/rs-7316280/v1/5e07f136d45e027980bc6af0.png"},{"id":90315723,"identity":"6d730efc-495e-42ee-967d-85b87fb1a83c","added_by":"auto","created_at":"2025-09-01 10:13:54","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":181735,"visible":true,"origin":"","legend":"\u003cp\u003eAmino acid stimulation induces mTORC1 activation and dissociation of LARS1 from IARS1.\u003cstrong\u003e \u003c/strong\u003eHEK293T cells expressing FLAG-LARS1 were starved of amino acids for 24 h, then stimulated with complete amino acids. (\u003cstrong\u003ea\u003c/strong\u003e) Western blot showing time-dependent phosphorylation of S6K1 (Thr389), total S6K1, FLAG-LARS1, and GAPDH. Quantitative analysis of phospho-S6K1 to total S6K1 ratio following amino acid stimulation is shown (right). (\u003cstrong\u003eb\u003c/strong\u003e) Co-immunoprecipitation of IARS1 with FLAG-LARS1 over time after stimulation and quantification of IARS1 co-precipitation with LARS1 (right). (\u003cstrong\u003eC\u003c/strong\u003e) MS/MS analysis reveals amino acid-induced phosphorylation of the LARS1 UNE-L domain. MS/MS spectrum confirming simultaneous phosphorylation at Ser1070, Ser1077, and Ser1082 residues in the UNE-L domain following amino acid stimulation. The peptide sequence and fragment ions (b-ions and y-ions) are labeled.\u003c/p\u003e","description":"","filename":"3.png","url":"https://assets-eu.researchsquare.com/files/rs-7316280/v1/fbd7ac385772fa3ad9a712cf.png"},{"id":90315731,"identity":"b0623616-a5ee-4c8e-87be-dd43a14963fa","added_by":"auto","created_at":"2025-09-01 10:13:55","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":225198,"visible":true,"origin":"","legend":"\u003cp\u003ePhosphorylation sites in the UNE-L domain and their functional validation. (\u003cstrong\u003ea\u003c/strong\u003e) Phosphorylation site serines (Ser1070, Ser1077, Ser1082) and their interacting residues potentially affected by phosphorylation. Distances between the serine side chain hydroxyl oxygens and interacting atoms are indicated by dashed lines (yellow: hydrogen bonds, gray: potential electrostatic repulsion upon phosphorylation). (\u003cstrong\u003eb\u003c/strong\u003e) \u003cem\u003eIn vitro\u003c/em\u003e pull-down assay using phosphoserine-containing UNE-L domains generated via genetic code expansion. Phosphatase treatment restores IARS1 binding. (\u003cstrong\u003ec\u003c/strong\u003e) Co-immunoprecipitation showing reduced IARS1 binding by LARS1 phosphomimetic mutants. (\u003cstrong\u003ed\u003c/strong\u003e) mTORC1 activation, measured as the phospho-S6K1/S6K1 ratio, in cells expressing various LARS1 mutants. Values are normalized to wild-type LARS1. Statistical significance was determined by an unpaired Student's t-test (n=4, mean ± SEM, **p\u0026lt;0.01, ***p\u0026lt;0.001).\u003c/p\u003e","description":"","filename":"4.png","url":"https://assets-eu.researchsquare.com/files/rs-7316280/v1/37d4fba96931c70e22e5daa6.png"},{"id":90315727,"identity":"9710d134-1367-43a2-8354-373ff9ab54c8","added_by":"auto","created_at":"2025-09-01 10:13:54","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":156233,"visible":true,"origin":"","legend":"\u003cp\u003eModel of the LARS1-mediated mTORC1 activation pathway. In this schematic model, MSC is drawn by a blob with a detailed Subcomplex III model including EPRS1, IARS1, and LARS1. Amino acid-induced phosphorylation of the LARS1 UNE-L domain triggers its dissociation from IARS1 within the MSC, lysosomal translocation, and subsequent mTORC1 activation through RagD:GTP hydrolysis and VPS34 interaction.\u003c/p\u003e\n\u003cp\u003eFigure created with BioRender.com\u003c/p\u003e","description":"","filename":"5.png","url":"https://assets-eu.researchsquare.com/files/rs-7316280/v1/c439592ef9e2e9e6b4414fab.png"},{"id":90318020,"identity":"16b865f2-1421-4cc5-b3bf-7442431ce027","added_by":"auto","created_at":"2025-09-01 10:29:55","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":2260077,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7316280/v1/d769f2f5-7f2d-44f1-bad9-b17775f10d12.pdf"},{"id":90316531,"identity":"b698aa84-6a22-4378-80d1-189d1762387a","added_by":"auto","created_at":"2025-09-01 10:21:54","extension":"docx","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":6201463,"visible":true,"origin":"","legend":"Cryo-EM Structure of the LARS1-IARS1 Complex Reveals A Nutrient-Responsive Switch Controlling mTORC1 Signaling","description":"","filename":"phosphoLARSSuppl080725.docx","url":"https://assets-eu.researchsquare.com/files/rs-7316280/v1/ac1acbf836d263fa0a51a8c5.docx"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"\u003cp\u003e\u003cstrong\u003eCryo-EM Structure of the LARS1-IARS1 Complex Reveals A Nutrient-Responsive Switch Controlling mTORC1 Signaling\u003c/strong\u003e\u003c/p\u003e","fulltext":[{"header":"1. Introduction","content":"\u003cp\u003eAminoacyl-tRNA synthetases (ARSs) are indispensable enzymes that attach specific amino acids to their corresponding tRNAs, which is the fundamental step in translating the genetic code into proteins\u003csup\u003e\u003cspan additionalcitationids=\"CR2 CR3\" citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e\u003c/sup\u003e. While their canonical function is conserved across species, ARSs exhibit striking variability in structure and oligomeric states\u003csup\u003e\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e\u003c/sup\u003e, implying their diverse and context-dependent roles beyond aminoacylation\u003csup\u003e\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e\u003c/sup\u003e. Over the course of evolution, many ARSs have acquired additional domains that enable interactions with other synthetases and cellular proteins, allowing them to participate in various regulatory and signaling pathways. These structural adaptations, along with the integration of scaffold proteins, have led to the formation of multi-tRNA synthetase complexes (MSCs) with species-specific compositions\u003csup\u003e\u003cspan additionalcitationids=\"CR7\" citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e\u003c/sup\u003e. In humans, eight cytosolic ARSs (glutamyl-prolyl, isoleucyl, leucyl, methionyl, glutaminyl, arginyl, lysyl, and aspartyl tRNA synthetases) form MSC with three scaffold proteins known as aminoacyl-tRNA synthetase-interacting multifunctional proteins (AIMP1/p43, AIMP2/p38, and AIMP3/p18)\u003csup\u003e9\u003c/sup\u003e. The MSC functions as a central hub that orchestrates the non-canonical activities of its components\u003csup\u003e\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e\u003c/sup\u003e, including roles in cell signaling, apoptosis, immune modulation, and stress sensing. Recent comprehensive reviews have highlighted the involvement of various ARSs in tumorigenesis, emphasizing their potential as therapeutic targets and diagnostic markers\u003csup\u003e\u003cspan additionalcitationids=\"CR12 CR13\" citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e\u003c/sup\u003e.\u003c/p\u003e\u003cp\u003eAmong MSC components, leucyl-tRNA synthetase 1 (LARS1) stands out as one of the most functionally significant, due to its role as a leucine sensor in the mammalian target of rapamycin complex 1 (mTORC1) signaling pathway\u003csup\u003e\u003cspan additionalcitationids=\"CR16 CR17 CR18\" citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e\u003c/sup\u003e. Beyond its canonical function of charging tRNA\u003csup\u003eLeu\u003c/sup\u003e, LARS1 acts as a leucine-responsive GTPase-activating protein (GAP) that directly binds and activates Ras-related GTP-binding protein D (RagD)\u003csup\u003e\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e\u003c/sup\u003e. LARS1 also activates vacuolar protein sorting 34 (VPS34) in response to amino acid signals\u003csup\u003e\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e\u003c/sup\u003e, where both RagD and VPS34 promote mTORC1 activation - a key regulatory node for cell growth, metabolism, and autophagy\u003csup\u003e\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e\u003c/sup\u003e. As such, LARS1 has become a focal point in studies of nutrient sensing, metabolic disorders, and cancer biology, particularly due to the therapeutic relevance of mTORC1 signaling. Despite these findings, several critical gaps remain regarding the regulation of the non-canonical functions of LARS1 and, by extension, the components of MSC. First, while LARS1 is known to translocate to the mTORC1 on lysosomal surfaces upon leucine stimulation, the molecular mechanisms underlying this dynamic relocalization are poorly understood. In particular, it remains unclear whether this transition is initiated by specific events such as post-translational modifications, proteolytic cleavage, or other stimulus-dependent mechanisms. Second, it is still uncertain whether LARS1 must dissociate from the MSC to activate mTORC1. Finally, the high-resolution structural organization of LARS1 within the MSC remains unresolved, although IARS1 has been reported to be the linker of LARS1 to the MSC\u003csup\u003e\u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e,\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e\u003c/sup\u003e.\u003c/p\u003e\u003cp\u003eIn this study, we determined the cryo-electron microscopy (cryo-EM) structure of the LARS1:IARS1 complex and investigated the regulatory mechanisms that govern LARS1-mediated non-canonical signaling within the MSC. Structural and mass spectrometry analysis revealed phosphorylation sites at the LARS1:IARS1 interface that may regulate their interaction. Further experiments revealed that these phosphorylation events are triggered by leucine stimulation and modulate LARS1\u0026rsquo;s association with the MSC, ultimately leading to mTOR1 activation. Our findings advance our understanding of how LARS1 transduces amino acid signals to mTOR1 and provide key insights into how the MSC dynamically mobilizes its components for context-dependent, non-canonical cellular functions.\u003c/p\u003e"},{"header":"2. Results and Discussion","content":"\u003cp\u003e\u003cstrong\u003eLARS1:IARS1 association is mediated by LARS1 UNL-L domain\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eSince IARS1\u0026nbsp;is known to be the linker connecting LARS1 to the MSC complex\u003csup\u003e24,25\u003c/sup\u003e, we aimed to determine the LARS1:IARS1 complex structure to gain insight into the spatial organization of LARS1 within the MSC. To do this, human\u0026nbsp;LARS1 and IARS1, both in full-length form, were co-expressed in \u003cem\u003eE.\u003c/em\u003e\u003cem\u003e\u0026nbsp;coli\u003c/em\u003e BL21 (DE3) and purified using affinity and size exclusion chromatography. IARS1, which is unstable when expressed alone, becomes stabilized through complex formation with LARS1, thereby facilitating the purification of the complex. The cryo-EM structure of the LARS1:IARS1 complex was determined at 3.2 Å resolution (Fig. 1, Table 1, Supplementary Figs. 1 and 2). Although full-length proteins were used, the resulting density map resolved only partial regions of both components. For the IARS1, most catalytic region, including the connective peptide (CP), catalytic domain (CD), and anticodon-binding domain (ABD), was visualized, while only the UNE-L domain was observed in the LARS1. Most of the catalytic region of the LARS1 and the C-terminal junction and UNE-I domains of the IARS1 were not resolved, demonstrating the high mobility of these domains compared to the resolved region (Fig. 1a, b). During cryo-EM data processing, we identified a few 2D classes that display additional density to the visualized LARS1:IARS1 complex (Fig. 1c). We generated a 3D reconstruction using these 2D classes, and could observe the core module of LARS1 connected to the UNE-L domain, although the map resolution was low, hindering the detailed structural analysis of the complex (Fig. 1c). The Rag-binding domain (RBD) of LARS1 was positioned adjacent to the UNE-L domain in the map, however, it did not interact directly with IARS1, confirming that only UNE-L domain forms a stable interface with IARS1 as suggested by previous studies using size exclusion chromatography and XL-MS analysis\u003csup\u003e24,26\u003c/sup\u003e.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe interface between LARS1 and IARS1 involves the CD and CP3 domains of IARS1 and the UNE-L domain of LARS1, forming a contact surface of approximately 1140 Å\u003csup\u003e2\u003c/sup\u003e, as analyzed by ePISA\u003csup\u003e27\u003c/sup\u003e (Supplementary Table 1). The interactions at this interface include hydrogen bonds, salt bridges, and hydrophobic contacts (Fig. 2a, Supplementary Tables 2, 3, and 4). Multiple sequence alignment (MSA) combined with structural analysis revealed that the key IARS1 interface residues are well-conserved among species whose LARS1s contain the UNE-L domain (Figs. 2b, c). In contrast, the corresponding residues, especially within the CP3 domain, are poorly conserved in the IARS1 of the invertebrates, which lacks the LARS1 UNE-L domain. This suggests that the LARS1:IARS1 interaction evolved after the emergence of vertebrates and has been conserved in species that harbor UNE-L domains in their LARS1. Furthermore, to gain insight into the dynamic behavior of interface residues and to identify functionally important residues not resolved in the cryo-EM structure, we performed molecular dynamics (MD) simulations (Supplementary Fig. 3). The simulation results were consistent with the cryo-EM analysis, showing that residues involved in hydrogen bonding, salt bridge formation, and the hydrophobic core in the structure significantly contribute to the binding enthalpy. Notably, the highly conserved residues in the IARS1 interface, Asn1076, Phe1081, and Arg1126, showed larger enthalpic contributions than other interfacial residues. This convergence of data from structural analysis, MD simulations, and multiple sequence alignment underscores the consistency and significance of these findings.\u003c/p\u003e\n\u003cp\u003eThe cryo-EM density map of the LARS1:IARS1 complex also includes the first high-resolution structure of human IARS1. Previously, the only known eukaryotic IARS1 structures were those from \u003cem\u003eCandida albicans\u003c/em\u003e and \u003cem\u003eSaccharomyces cerevisiae\u003c/em\u003e\u003csup\u003e28,29\u003c/sup\u003e. Despite species differences, these structures exhibit a conserved domain architecture with the human homolog, comprising the CD, which facilitates aminoacylation of tRNA\u003csup\u003eIle\u003c/sup\u003e, the CP domain, which mediates interdomain flexibility and serves as a platform for protein–protein interactions, and the ABD, responsible for recognizing and stabilizing the tRNA anti-codon loop (Supplementary Fig. S4). Structural comparisons yielded low RMSD(Cα) values of 1.337 Å (with \u003cem\u003eC. albicans\u003c/em\u003e, PDB: 6LDK) and 1.879 Å (with \u003cem\u003eS. cerevisiae\u003c/em\u003e, PDB: 8WND), indicating strong conservation across species.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAmino acid stimulation triggers dissociation of LARS1 from the MSC\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eIt remains unclear whether LARS1 must dissociate from the MSC to exert its noncanonical function, such as mTORC1 activation. Since IARS1 is the binding partner anchoring LARS1 to the MSC\u003csup\u003e25\u003c/sup\u003e, we investigated whether LARS1’s role in amino acid-dependent mTORC1 activation requires the dissociation of the LARS1:IARS1 complex. Previous studies reported that LARS1, but not IARS1, colocalizes with mTOR and Raptor upon amino acid stimulation, suggesting that its dissociation from IARS1 may underlie relocalization and signaling activation\u003csup\u003e15\u003c/sup\u003e.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eTo directly test whether amino acid stimulation affects LARS1:IARS1 interaction, we performed time-course analyses assessing both downstream pathway activation and complex integrity (Figs. 3a, b). HEK293T cells overexpressing FLAG-tagged LARS1 were subjected to amino acid starvation for 24 hours. The medium was then replaced with one containing complete amino acids, and the cells were harvested at different time points for analysis. The mTORC1 activation was measured by the phosphorylation of Thr389 on ribosomal protein S6 kinase beta-1 (S6K1), a well-established downstream target of mTORC1. The mTORC1 activity showed a sharp increase within the first hour and remained elevated throughout the 4-hour observation period (Fig. 3a). In parallel, immunoprecipitation (IP) of LARS1 followed by western blot analysis revealed a rapid decline in LARS1:IARS association within the first hour after amino acid stimulation, which was sustained at low levels for the remainder of the observation period (Fig. 3b). The temporal coincidence and parallel patterns of mTORC1 activation and LARS1 dissociation suggest that LARS1 liberation from the MSC may be functionally associated with activation of the mTORC1 signaling pathway.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAmino acid stimulation induces phosphorylation of the LARS1 UNE-L domain\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eLARS1 is known to undergo a conformational change that facilitates its interaction with RagD upon binding of leucine\u003csup\u003e20\u003c/sup\u003e. Having established that LARS1 dissociates from IARS1 upon an amino acid stimulus, we next investigated whether this dissociation results from direct amino acid binding. To test this, we co-expressed human LARS1 with GST-tagged IARS1 in \u003cem\u003eE. coli\u0026nbsp;\u003c/em\u003eBL21 (DE3), purified the complex, and incubated it with either an excess amount of leucine, isoleucine, or ATP (Supplementary Fig. 5). Subsequent glutathione resin pull-down assays showed no significant difference in the amount of LARS1 co-precipitating with IARS1 under these conditions, implying that additional layer of cellular signaling is required to trigger LARS1 dissociation, as observed in the cell-based assays (Figs. 3a, b).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eAs potential signals triggering LARS1 dissociation from the MSC, proteolytic cleavage and specific post-translational modifications (PTMs) have been shown to regulate\u0026nbsp;the subcellular localization and functional repertoire of MSC components\u003csup\u003e13\u003c/sup\u003e. Several MSC components, including AIMP1\u003csup\u003e30\u003c/sup\u003e, QARS\u003csup\u003e31\u003c/sup\u003e, KARS\u003csup\u003e32\u003c/sup\u003e, and EPRS\u003csup\u003e33\u003c/sup\u003e, undergo protease-mediated cleavage that facilitates their release from the complex and subsequent engagement in non-canonical functions. Phosphorylation has also been implicated in modulating the noncanonical roles of MSC components such as EPRS\u003csup\u003e34\u003c/sup\u003e, KARS\u003csup\u003e35\u003c/sup\u003e, MARS\u003csup\u003e36\u003c/sup\u003e, and IARS1\u003csup\u003e37\u003c/sup\u003e. To determine whether LARS1 is subject to similar regulation, HEK293T cells expressing FLAG-tagged LARS1 were subjected to amino acid starvation for 24 hours, followed by stimulation with either 2 mM leucine or a complete amino acid mixture (Supplementary Fig. 6). Western blot analysis confirmed increased mTORC1 activity following both leucine and total amino acid stimulation. Notably, SDS-PAGE analysis of the immunoprecipitated FLAG-LARS1 showed no gel band shift before or after amino acid stimulation, thereby ruling out the possibility of proteolytic cleavage as a regulatory mechanism.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eWe next conducted mass spectrometry (MS) analysis on immunoprecipitated LARS1 to identify PTMs associated with amino acid stimulation. The analysis revealed distinct phosphorylation patterns depending on the nutritional conditions (Figure 3c, Supplementary Fig. 7). Specific phosphorylation sites were detected exclusively under amino acid starvation, while others appeared only upon amino acid stimulation. For example, phosphorylation at Ser720, located within a KMSKS motif and previously reported as a target of Unc-51-like autophagy-activating kinase 1 (ULK1)\u003csup\u003e38\u003c/sup\u003e, was detected specifically under amino acid starvation but was absent following amino acid stimulation. Notably, amino acid stimulation triggered multiple phosphorylation events within the UNE-L domain (residues 1064-1176), particularly at the LARS1:IARS1 interface (Figs. 2a, Supplementary Fig.7). MS/MS spectra identified a peptide triply phosphorylated at Ser1070, Ser1077, and Ser1082, as confirmed by four independent peptide spectrum matches (PSMs) (Figure 3c). Phosphorylation at Ser1068 was also detected, but at a lower frequency, indicating it is a minor site. These results suggest that the clustered phosphorylation sites on the UNE-L domain function as a potential regulatory hotspot governing the LARS1’s association with IARS1, presumably in the MSC.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003ePhosphorylation of the LARS1 UNE-L domain disrupts the LARS1:IARS1 complex\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe then conducted both \u003cem\u003ein vitro\u003c/em\u003e and cellular experiments to assess how phosphorylation of the UNE-L domain affects the integrity of the LARS1:IARS1 complex. To precisely control the phosphorylation state at specific serine residues, we employed a genetic code expansion technique\u003csup\u003e39,40\u003c/sup\u003e, which enables site-specific incorporation of \u003cem\u003eO\u003c/em\u003e-phosphoserine during protein synthesis. This system utilizes an orthogonal translation machinery consisting of phosphoseryl-tRNA synthetase (SepRS), an amber suppressor tRNA (tRNA\u003csup\u003eSep\u003c/sup\u003e), and an engineered elongation factor for phosphoseryl-tRNA (EF-Sep). This approach allows for precise phosphorylation at a targeted site without requiring prior knowledge of the responsible kinase. Since UNE-L is the only LARS1 domain involved in complex formation with IARS1, we produced C-terminal His\u003csub\u003e6\u003c/sub\u003e-tagged UNE-L (LARS1 residues 1064-1176) with phosphorylation at Ser1070, Ser1077, or Ser1082 (Fig. 4a, Supplementary Fig. 8). Ser1068 was excluded because it is located relatively distant from the LARS1:IARS1 interface compared to the other sites. The phosphorylation status of the produced proteins was verified by mass spectrometry (Supplementary Figs. 9, 10). To assess the effect of phosphorylation on IARS1 binding, we conducted pull-down assays using GST-IARS1 fusion protein and UNE-L proteins with different phosphorylation states (Fig. 4b). The UNE-L variants were mixed with GST-IARS1 and incubated on ice for 1 hour, followed by GST pull-down assays. Wild-type UNE-L was efficiently pulled down with GST-IARS1, as expected. In contrast, the phosphorylated UNE-L variants (Ser1070Sep, Ser1077Sep, and Ser1082Sep) showed significantly reduced binding. Furthermore, when phosphorylated LARS1 UNE-L variants were dephosphorylated by lambda phosphatase at 30°C for 30 minutes, the binding defect was reversed. This indicates that phosphorylation at these sites is sufficient to disrupt LARS1:IARS1 complex formation \u003cem\u003ein vitro\u003c/em\u003e.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eTo further investigate the impact of phosphorylation at the LARS1 UNE-L domain in a cellular context, FLAG-tagged full-length wild-type LARS1 or various phosphomimetic variants (Ser1070Glu, Ser1077Glu, Ser1082Glu, Ser1077/1082Glu, and Ser1070/1077/1082Glu) were transfected into HEK293T cells. A co-immunoprecipitation (co-IP) assay was then performed using an anti-FLAG antibody to pull down FLAG-LARS1 and detect associated IARS1. Consistent with the \u003cem\u003ein vitro\u003c/em\u003e findings, only the wild-type LARS1 co-immunoprecipitated with IARS1, while the phosphomimetic variant, whether single-site, double-site, or triple-site, failed to pull down IARS1 (Fig. 4c). Combined with the mass spectrometry data, these results demonstrate that phosphorylation of the LARS1 UNE-L domain at residues Ser1070, Ser1077, and Ser1082, triggered by amino acid stimulation, is responsible for the dissociation of LARS1 from IARS1, and ultimately from the MSC.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003ePhosphomimetic mutants of the LARS1 UNE-L domain promote mTORC1 activation\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eHaving established the primary role of this phosphorylation in LARS1, we next examined whether phosphorylation-induced dissociation affects LARS1’s function in activating mTORC1 (Supplementary Fig. 11). Exogenous expression of wild-type LARS1 led to a modest increase in mTORC1 activity compared to the empty vector control, indicating that elevated cytosolic LARS1 induces only a marginal mTORC1 activation. In contrast, single phosphomimetic variants, Ser1077Glu and Ser1082Glu, triggered a pronounced increase in mTORC1 activity, up to threefold over empty vector control. The elevated activity observed with the Ser1082Glu variant was reduced when the phosphomimetic residue was substituted with alanine, supporting that phosphorylation at Ser1082 enhances mTORC1 activation beyond merely disrupting hydrogen bonding at the interface (Supplementary Fig. 11). Interestingly, although the single, double, and triple phosphomimetic variants all showed similar dissociation patterns from IARS1 in the co-IP assay, their effects on mTORC1 activation differed markedly (Fig. 4d). In particular, Ser1070Glu exhibited activity comparable to wild-type LARS1, whereas Ser1077Glu and Ser1082Glu exhibited robust activation. Of particular note, the triple mutant Ser1070/1077/1082Glu, which mimics the phosphorylation pattern observed upon amino acid stimulation, induced the highest level of mTORC1 activation, approximately 3.3-fold greater than wild-type LARS1. These results demonstrate that mTORC1 activation is not simply driven by the release of LARS1 from the MSC, but is critically modulated by specific phosphorylation events on LARS1. This is also consistent with the result that the UNE-L-deficient LARS1 showed reduced mTORC1 activation compared to the Ser1077Glu and Ser1082Glu mutants (Supplementary Fig. 11). Therefore, coordinated phosphorylation of the UNE-L domain likely functions as a key regulatory switch that not only promotes LARS1 dissociation from the MSC but also enhances its capacity to activate mTORC1, potentially via specific interactions with upstream effectors of the mTORC1 pathway.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eARS complex structures provide insights into the overall architecture of the MSC\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe complexation of ARSs into the MSC provides a functional platform for individual synthetases to carry out noncanonical roles in response to external stimuli. Although biochemical, crosslinking mass spectrometry, and molecular docking studies have provided insights into the three-dimensional organization of the human MSC\u003csup\u003e26\u003c/sup\u003e, its complete high-resolution structure remains unresolved. The human MSC is known to include eight ARS (EPRS1, IARS1, LARS1, MARS1, QARS1, RARS1, KARS1, and DARS1) and three scaffold proteins (AIMP1:p43, AIMP2:p38, AIMP3:18), organized into three subcomplexes\u003csup\u003e41\u003c/sup\u003e. Subcomplex I includes QARS1, RARS1, KARS1, AIMP1, and AIMP2; Subcomplex II contains MARS1, AIMP3, EPRS1, and AIMP2; Subcomplex III comprises EPRS1, IARS1, and LARS1.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eRecent high-resolution structural studies have provided critical insights into the organization of these subcomplexes. In Subcomplex I, KARS1 forms a dimer and interacts with the N-terminal region of AIMP2. In Subcomplex II, the MARS1\u003csub\u003eGST\u003c/sub\u003e:AIMP3\u003csub\u003eGST\u003c/sub\u003e:EPRS\u003csub\u003eGST\u003c/sub\u003e:AIMP2\u003csub\u003eGST\u003c/sub\u003e complex structure reveals a core architecture formed by GST-like domains, while the DARS1:AIMP2\u003csub\u003eGST\u003c/sub\u003e:EPRS\u003csub\u003eGST\u003c/sub\u003e complex structure further details its assembly\u003csup\u003e42,43\u003c/sup\u003e. In Subcomplex III, structural inshgits have emerged from both the crystal structures of the EPRS1:IARS1 complex and our cryo-EM structure of the LARS1-IARS1 complex. In the EPRS1:IARS1 complex, the EARS1 ABD interacts with the UNE-I domain of IARS1, with a hairpin loop from the ABD inserting into the cleft between UBX1 and UBX2 domains of the UNE-I\u003csup\u003e37\u003c/sup\u003e. Phosphomimetic mutations at two serine residues within this loop disrupt the interaction, suggesting that phosphorylation at these sites promotes the release of IARS1 from the MSC, a mechanism that parallels our findings for LARS1. Taken together, these structural findings, including our LARS1:IARS1 complex structure, enable the construction of a molecular model for MSC, and especially for Subcomplex III. While individual synthetases likely retain conformational flexibility, the components are interconnected through robust, domain-mediated interactions: UNE-L links LARS1 to IARS1, and UNE-I connects IARS1 to EPRS1 (Supplementary Fig. 12).\u003c/p\u003e"},{"header":"3. Discussion","content":"\u003cp\u003eBased on our structural, biochemical, and cellular findings, we propose a comprehensive model for LARS1 regulation and its role in amino acid sensing and mTORC1 activation (Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003e). Under amino acid starvation, a significant portion of LARS1 is sequestered within the MSC via interaction with IARS1, limiting its availability for non-canonical signaling functions. Upon amino acid stimulation, as yet unidentified nutrient-responsive kinases trigger multi-site phosphorylation of the LARS1 UNE-L domain. This phosphorylation event destabilizes the LARS1\u0026ndash;IARS1 interaction, shifting the equilibrium toward LARS1 dissociation from the MSC. Free cytosolic LARS1, when bound to leucine, has been reported to exhibit increased affinity for RagD:GTP and translocate to the lysosomal membrane\u003csup\u003e\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e,\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e\u003c/sup\u003e. There, it activates mTORC1 either by promoting the hydrolysis of RagD:GTP to RagD:GDP\u003csup\u003e\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e\u003c/sup\u003e or by activating VPS34, which has been shown to directly interact with the UNE-L domain of LARS1\u003csup\u003e21\u003c/sup\u003e. Given the critical role of UNE-L domain phosphorylation in mTORC1 activation, this modification likely acts as a key regulator that promotes LARS1's interaction with mTORC1-activating partners.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eThis study highlights phosphorylation as a critical molecular switch that regulates LARS1's non-canonical functions in mTORC1 activation, exemplifying how the MSC efficiently responds to external stimuli. Phosphorylation has also been observed in other MSC components, including MARS\u003csup\u003e\u003cspan citationid=\"CR36\" class=\"CitationRef\"\u003e36\u003c/span\u003e\u003c/sup\u003e, KARS\u003csup\u003e\u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e35\u003c/span\u003e,\u003cspan additionalcitationids=\"CR45 CR46 CR47 CR48 CR49 CR50 CR51 CR52\" citationid=\"CR44\" class=\"CitationRef\"\u003e44\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR53\" class=\"CitationRef\"\u003e53\u003c/span\u003e\u003c/sup\u003e, EPRS\u003csup\u003e\u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e34\u003c/span\u003e,\u003cspan additionalcitationids=\"CR55 CR56 CR57 CR58 CR59\" citationid=\"CR54\" class=\"CitationRef\"\u003e54\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR60\" class=\"CitationRef\"\u003e60\u003c/span\u003e\u003c/sup\u003e, and IARS1\u003csup\u003e37,61\u003c/sup\u003e, suggesting a conserved regulatory mechanism within the complex. This mechanism involves rapid activation of specific kinase(s) by an external cue, leading to the prompt dissociation of selected MSC components and subsequent activation of downstream signaling pathways. These observations contribute to a broader understanding of the MSC as a dynamic and modular signaling platform that integrates structural plasticity with stimulus-responsiveness, rather than functioning as a rigid, static megacomplex. This inherent structural flexibility may account for why elucidating the whole structure of the MSC has remained elusive. The present study is expected to lead to the identification of key nutrient-responsive kinase(s) responsible for phosphorylating critical sites in the LARS1-UNE-L domain, thereby activating mTORC1 signaling. These findings may ultimately guide the development of novel therapeutic strategies for cancer and metabolic disorders involving dysregulated mTORC1 activity.\u003c/p\u003e"},{"header":"4. Methods","content":"\u003cp\u003e\u003cb\u003ePlasmid construction and protein purification\u003c/b\u003e\u003c/p\u003e\u003cp\u003e\u003cb\u003eLARS1:IARS1 Complex\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThe full-length human LARS1 gene, without any affinity tag, was inserted into a pCDF-Duet vector between the NcoI and AscI restriction sites. The full-length human IARS1 gene was cloned as an N-terminal hexahistidine (His\u003csub\u003e6\u003c/sub\u003e)-glutathione S-transferase (GST) fusion with a Tobacco etch virus (TEV) protease cleavage site into the pET28a vector\u0026rsquo;s NcoI and XhoI restriction sites. Both recombinant genes were cloned using Gibson assembly (NEB). \u003cem\u003eEscherichia coli\u003c/em\u003e BL21(DE3) cells were sequentially transformed with both plasmids to co-express the LARS1:IARS1 complex. Transformed cells were cultured in 6.4 L of LB medium at 30\u0026deg;C until the optical density at 600 nm reached 0.6. Protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG). After induction, the temperature was lowered to 23\u0026deg;C, and cells were grown for an additional 18 hours before harvest. Cells were lysed by sonication in lysis buffer (20 mM Tris-HCl pH 8.0, 200 mM NaCl, 10 mM imidazole, 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM dithiothreitol (DTT)) supplemented with protease inhibitor cocktail (Roche). The cell lysate was clarified by centrifugation, and the supernatant was incubated with Ni-NTA agarose resin (Qiagen). The resin was washed with wash buffer (20 mM Tris-HCl pH 8.0, 200 mM NaCl, 40 mM imidazole, 1 mM DTT), and the bound proteins were eluted with elution buffer (20 mM Tris-HCl pH 8.0, 200 mM NaCl, 250 mM imidazole, 1 mM DTT). The eluted proteins were incubated with TEV protease at a 1:100 (w/w) ratio and dialyzed against dialysis buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 14 mM β-mercaptoethanol) to remove the His\u003csub\u003e6\u003c/sub\u003e-GST tag. After cleavage, the protein solution was concentrated using a 100 kDa cut-off ultracentrifugal filter (Amicon). The LARS1:IARS1 complex was isolated by size exclusion chromatography using a Superdex\u0026trade; 200 increase 10/300 GL column (Cytiva) equilibrated with SEC buffer (20 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM DTT). Peak fractions containing the LARS1:IARS1 complex were collected and concentrated using a 100 kDa ultracentrifugal filter (Amicon) for cryo-EM grid preparation.\u003c/p\u003e\u003cp\u003e\u003cb\u003ePhospho-UNE-L domain\u003c/b\u003e\u003c/p\u003e\u003cp\u003eTo produce wild-type UNE-L protein (UNE-L-WT), the plasmid pCDF-Duet-UNE-L-WT was constructed by cloning the gene for human LARS1 UNE-L domain (residues 1064\u0026ndash;1176) with an N-terminal His\u003csub\u003e6\u003c/sub\u003e-tag between the NcoI and AscI sites of pCDF-Duet (Novagen). For the production of UNE-L domains with a phosphoserine (Sep) at specific positions, phosphoserine variants Ser1070Sep, Ser1077Sep, and Ser1082Sep were generated by introducing amber stop codons (TAG) at the corresponding positions via overlap PCR. The resulting PCR fragments were cloned into the pCDF-Duet SepT vector \u003csup\u003e\u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e40\u003c/span\u003e\u003c/sup\u003e. For the expression of phosphoserine-containing UNE-L variants, the corresponding plasmids were transformed into \u003cem\u003eE. coli\u003c/em\u003e BL21(DE3) cells carrying the advanced Sep incorporation system (pKD-SepRS9-EFSep21)\u003csup\u003e\u003cspan citationid=\"CR39\" class=\"CitationRef\"\u003e39\u003c/span\u003e,\u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e40\u003c/span\u003e\u003c/sup\u003e. Transformed cells were grown in 2xYT medium at 30\u0026deg;C until the optical density at 600 nm reached 0.8. The temperature was then lowered to 23\u0026deg;C, and protein expression was induced by adding 0.5 mM IPTG and incubating for 24 hours. Phosphoserine-containing UNE-L variants and wild-type UNE-L were purified by Ni-NTA agarose column chromatography. The eluted proteins were concentrated using 3 kDa cutoff ultracentrifugal filters (Amicon).\u003c/p\u003e\u003cp\u003e\u003cb\u003eGST-IARS1\u003c/b\u003e\u003c/p\u003e\u003cp\u003eFor GST-IARS1 protein purification, the sequence was cloned into a pET-21a vector using NcoI-AscI restriction sites. The recombinant plasmid was transformed into \u003cem\u003eE. coli\u003c/em\u003e BL21(DE3). Cells were grown in 6.4 L of LB medium at 37\u0026deg;C until the optical density at 600 nm reached 0.6. Then, protein expression was induced with 0.5 mM IPTG at 18\u0026deg;C for 18 hours. Cells were harvested by centrifugation, resuspended in phosphate-buffered saline (PBS), and lysed by sonication. After centrifugation, the clear supernatant was incubated with glutathione sepharose resin (GenScript) for 1 hour at 4\u0026deg;C. The resin was washed with PBS (10 column volumes \u0026times; 5 times), and the protein was eluted with 33 mM glutathione in 50 mM Tris-HCl buffer (pH 8.0).\u003c/p\u003e\u003cp\u003e\u003cb\u003eCryo-EM sample preparation\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThe purified LARS1:IARS1 complex at a concentration of 0.5-1 mg/mL was applied to C-Flat 1.2/1.3 3 Au holey carbon grids (Electron Microscopy Sciences) that had been glow-discharged for 40 seconds. A 3.5 \u0026micro;L aliquot of the sample was applied to the grid, blotted for 5 seconds at 4\u0026deg;C with 100% humidity using a Vitrobot Mark IV (Thermo Fisher Scientific), and plunge-frozen in liquid ethane.\u003c/p\u003e\u003cp\u003e\u003cb\u003eCryo-EM data acquisition\u003c/b\u003e\u003c/p\u003e\u003cp\u003eData was collected on a 300kV Titan Krios G2 electron microscope (Thermo Fisher Scientific) at the Pacific Northwest Center for Cryo-EM (PNCC). Micrographs were recorded using a Falcon 4 direct electron detector operating in electron counting mode. All data acquisition was performed automatically using EPU software (Thermo Fisher Scientific), with micrographs saved in the electron-event representation (EER) format. Micrographs were collected at a calibrated pixel size of 0.814 \u0026Aring; with a defocus range varying from \u0026minus;\u0026thinsp;0.8 \u0026micro;m to -2.5 \u0026micro;m at a total electron dose of 60 e\u003csup\u003e\u0026minus;\u003c/sup\u003e/\u0026Aring; for 3.8 seconds. A total of 7,504 micrographs were acquired for subsequent processing.\u003c/p\u003e\u003cp\u003e\u003cb\u003eCryo-EM data processing\u003c/b\u003e\u003c/p\u003e\u003cp\u003eAll data processing was performed using cryoSPARC 4.2\u003csup\u003e62\u003c/sup\u003e. Movie frames were motion-corrected using patch motion correction in cryoSPARC. The contrast transfer function (CTF) parameters were estimated using patch CTF estimation in cryoSPARC. Particles were automatically picked using the blob picker in cryoSPARC, yielding approximately 4.69\u0026nbsp;million particles. The extracted particles were subjected to multiple rounds of 2D classification in cryoSPARC, resulting in 947,600 selected particles. Initial 3D model generation was performed using ab initio reconstruction (n\u0026thinsp;=\u0026thinsp;4) in cryoSPARC, which yielded a model consistent with the expected LARS1:IARS1 complex. The particles were further classified using heterogeneous refinement with four classes (n\u0026thinsp;=\u0026thinsp;4), using the same ab initio map as the reference. Among these classes, 135,800 particles were selected and subjected to homogeneous refinement, and further to non-uniform refinement, yielding a final resolution of 3.16 \u0026Aring;.\u003c/p\u003e\u003cp\u003e\u003cb\u003eModel building and refinement\u003c/b\u003e\u003c/p\u003e\u003cp\u003eFor initial model building, AlphaFold3 webserver was used to predict the structures of human IARS1 (residues 1-800) and the human LARS1 UNE-L domain (residues 1067\u0026ndash;1176) based on their respective amino acid sequences\u003csup\u003e\u003cspan citationid=\"CR63\" class=\"CitationRef\"\u003e63\u003c/span\u003e\u003c/sup\u003e. These predicted models were fitted into the cryo-EM density map using UCSF Chimera (version 1.11.12)\u003csup\u003e\u003cspan citationid=\"CR64\" class=\"CitationRef\"\u003e64\u003c/span\u003e\u003c/sup\u003e. The initial models were then docked into the density map using the Dock_in_map utility in PHENIX (version 1.18.2)\u003csup\u003e\u003cspan citationid=\"CR65\" class=\"CitationRef\"\u003e65\u003c/span\u003e\u003c/sup\u003e. The model was iteratively refined by PHENIX real-space refinement and manual adjustment in Coot (version 0.8.9)\u003csup\u003e\u003cspan citationid=\"CR66\" class=\"CitationRef\"\u003e66\u003c/span\u003e\u003c/sup\u003e. The final model was validated using the MolProbity web server\u003csup\u003e\u003cspan citationid=\"CR67\" class=\"CitationRef\"\u003e67\u003c/span\u003e\u003c/sup\u003e. The figures were made using UCSF ChimeraX (version 1.9)\u003csup\u003e\u003cspan citationid=\"CR68\" class=\"CitationRef\"\u003e68\u003c/span\u003e\u003c/sup\u003e.\u003c/p\u003e\u003cp\u003e\u003cb\u003eGST Pull-down assay\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThe mixture of GST-IARS1 and UNE-L domain samples, either treated with or without lambda phosphatase, was incubated with glutathione sepharose resin (GenScript) at 4\u0026deg;C for 2 hours. The resin was washed with 10 column volumes of PBS five times, and the GST-tagged protein was eluted with 33 mM glutathione in 50 mM Tris-HCl buffer (pH 8.0).\u003c/p\u003e\u003cp\u003e\u003cb\u003eMammalian cell culture and treatment\u003c/b\u003e\u003c/p\u003e\u003cp\u003eLARS1 and its mutants were cloned into a pcDNA3.1 vector with an N-terminal FLAG epitope tag (DYKDDDDK) for expression in mammalian cells. HEK293T cells were transfected with each plasmid using AccuFect\u0026trade; reagent (Bioneer), following the manufacturer\u0026rsquo;s instructions. Wild-type and transgenic HEK293T cells were cultured in Dulbecco\u0026rsquo;s Modified Eagle\u0026rsquo;s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37\u0026deg;C with 5% CO\u003csub\u003e2\u003c/sub\u003e. For amino acid starvation, cells were washed twice and incubated in amino acid-free DMEM for 24 hours. Amino acid stimulation was performed by adding DMEM containing total amino acids for the indicated time.\u003c/p\u003e\u003cp\u003e\u003cb\u003eCell lysis, immunoprecipitation, and immunoblot assays\u003c/b\u003e\u003c/p\u003e\u003cp\u003eTransfected HEK293T cells were washed with cold PBS and lysed in RIPA buffer containing benzonase (Enzynomics), protease inhibitor cocktail (Roche), and phosphatase inhibitor cocktail (Roche) at 4\u0026deg;C for 3 hours. Cell lysates were centrifuged at 13,000 rpm for 30 min at 4\u0026deg;C to obtain clear supernatants. Immunoprecipitation for FLAG-LARS1 was performed by incubating the cleared supernatants with anti-DYKDDDDDK G1 affinity resin (GenScript) at 4\u0026deg;C for 2 hours under agitation. The beads were then washed three times with PBS containing 0.1% NP-40. The washed agarose beads were either loaded directly onto an SDS-PAGE gel or subjected to additional purification. Proteins were eluted using PBS supplemented with 5 mM 3\u0026times;FLAG peptide (MedChemExpress). The eluted protein complexes were analyzed by SDS-PAGE and then subjected to Western blotting or mass spectrometry analysis.\u003c/p\u003e\u003cp\u003eProteins were transferred to PVDF membranes using a semi-dry transfer method with the Trans-Blot Turbo Transfer System (Bio-Rad) at 20 V for 1 hour. Proteins transferred were probed with corresponding antibodies, and immunoreactive bands were visualized using chemiluminescence. The antibodies used for immunoblot analysis included the following: P-S6K1 (Cell Signaling Technology, #9205S), total S6K1 (Cell Signaling Technology, #9202S), FLAG (Cell Signaling Technology, #8146S), GAPDH (Cell Signaling Technology, #2118S), and IARS11 (Abcam, ab31533). Immunoblot quantification was performed by measuring relative band intensities using ImageJ software\u003csup\u003e\u003cspan citationid=\"CR69\" class=\"CitationRef\"\u003e69\u003c/span\u003e\u003c/sup\u003e, with normalization as indicated.\u003c/p\u003e\u003cp\u003e\u003cb\u003eMALDI-MS/MS analysis\u003c/b\u003e\u003c/p\u003e\u003cp\u003eProteins pulled down with the anti-FLAG resin were subjected to SDS-PAGE. Following electrophoresis, corresponding bands were immediately excised using a clean scalpel. The gel slices were transferred into 1.5 mL low-peptide-binding microtubes and processed for destaining and dehydration as previously described\u003csup\u003e\u003cspan citationid=\"CR70\" class=\"CitationRef\"\u003e70\u003c/span\u003e\u003c/sup\u003e. A sufficient amount of trypsin solution was added to cover the dried gel. The samples were placed on ice to facilitate gel immersion, with vortexing and brief centrifugation every 10 min. After 60 min, 50 mM ammonium bicarbonate solution was added until the gel became transparent. The samples were incubated overnight at 37\u0026deg;C for tryptic digestion.\u003c/p\u003e\u003cp\u003eFollowing digestion, 1 \u0026micro;L of peptide extract was spotted onto 1 \u0026micro;L of pre-crystallized matrix on a 384-well-polished stainless-steel target (Bruker Daltonics). The analyses were performed using either α-cyano-4-hydroxycinnamic acid (10 mg/mL), sinapinic acid (10 mg/mL), or 2,5-dihydroxybenzoic acid (20 mg/mL) matrices. The spotted mixtures were allowed to air dry at room temperature. Mass spectrometry analysis was performed using a Bruker Autoflex Speed TOF/TOF mass spectrometer (Bruker Daltonics) at KAIST Analysis Center for Research Advancement (KARA). Mass spectra were obtained in positive-ion detection mode with reflectors, and each mass spectrum was generated by superimposing ten sets of 2,000 laser scans. For MALDI-TOF/TOF MS/MS analysis, the Laser Ionization Fragmentation Technology (LIFT) mode was used, with an initial accelerating voltage of 6 kV and a subsequent accelerating voltage of 19 kV for the LIFT cell. The detection frequency was set to 200 Hz. Each mass spectrum was derived from three superimpositions of 500 laser scans each. The acquired mass spectra were processed using FlexAnalysis software (version 3.3.65.0) (Bruker Daltonics).\u003c/p\u003e\u003cp\u003e\u003cb\u003eLC-MS/MS\u003c/b\u003e\u003c/p\u003e\u003cp\u003eFLAG-tagged LARS1 protein was immunoprecipitated from HEK293T cell lysates using anti-FLAG agarose resin. The resin was washed three times with PBS (10 column volumes each). Following washing, the resin was mixed with 6x SDS-PAGE loading dye (LPS Solutions) and heated at 95\u0026deg;C for 10 minutes to elute the proteins. After cooling, the samples were subjected to SDS-PAGE separation. Protein bands corresponding to the expected molecular weight of LARS1 were excised for in-gel digestion\u003csup\u003e\u003cspan citationid=\"CR70\" class=\"CitationRef\"\u003e70\u003c/span\u003e\u003c/sup\u003e. Following tryptic digestion, peptides were desalted using Graphite Spin Columns (Thermo Fisher Scientific) according to the manufacturer's protocol. The desalted peptides were resuspended in 0.1% trifluoroacetic acid (TFA) and stored at -20\u0026deg;C until LC-MS/MS analysis.\u003c/p\u003e\u003cp\u003eLC-MS/MS analysis was performed using a Vanquish\u0026trade; Neo UHPLC System coupled with an Orbitrap Eclipse\u0026trade; Tribrid\u0026trade; Mass Spectrometer (Thermo Fisher Scientific) at KARA. Peptides were first loaded onto an Acclaim\u0026trade; PepMap\u0026trade; 100 C18 LC Trap Column (75 \u0026micro;m \u0026times; 2 cm, 3 \u0026micro;m, 100 \u0026Aring;) and then separated on a Double nanoViper\u0026trade; PepMap\u0026trade; Neo Analytical Column (2 \u0026micro;m, C18, 75 \u0026micro;m \u0026times; 500 mm, 1500 bar) (Thermo Fisher Scientific). The mobile phases consisted of 0.1% formic acid in water (solvent A) and 0.1% formic acid in 80% acetonitrile (solvent B). Peptides were eluted using a linear gradient from 5\u0026ndash;40% solvent B over 80 minutes at a flow rate of 300 nL/min.\u003c/p\u003e\u003cp\u003eMS data were acquired in positive ion mode with a resolution of 240,000 at m/z\u0026thinsp;=\u0026thinsp;200. For data-dependent acquisition (DDA), the instrument operated in Top 3-second cycle mode. Full MS scans were acquired over a mass range of m/z 375\u0026ndash;1500 with an automatic gain control (AGC) target value of 250% and a maximum injection time was set to auto. Precursor ions exceeding an intensity threshold of 2.0e5 were selected for fragmentation. Fragmentation was performed using higher-energy collisional dissociation (HCD) with a normalized collision energy of 30%. MS/MS spectra were acquired with an AGC target of 5.0 \u0026times; 10\u003csup\u003e4\u003c/sup\u003e, a maximum injection time set to auto, and an isolation window of 1.6 m/z. Dynamic exclusion was set to 60 seconds to minimize repeated selection of precursors.\u003c/p\u003e\u003cp\u003eRaw data files were processed using Proteome Discoverer 3.1 (Thermo Fisher Scientific). Database searches were performed against the UniProt \u003cem\u003eHomo sapiens\u003c/em\u003e database (Proteome ID. UP000005640) and human LARS1 sequence (Uniprot Entry: Q9P2J5) using trypsin as the digestion enzyme. Post-translational modifications were searched using Sequest HT node (precursor tolerance: 0.1 Da, fragment tolerance: 0.05 Da). Static modifications included carbamidomethylation of cysteine (+\u0026thinsp;57.021 Da). Dynamic modifications included oxidation of methionine (+\u0026thinsp;15.995 Da), acetylation of protein N-termini (+\u0026thinsp;42.011 Da), and phosphorylation of serine, threonine, and tyrosine residues (+\u0026thinsp;79.966 Da) for phosphorylation studies\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAcknowledgements\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe thank Sung Gyu Lee (KARA) and\u0026nbsp;Marcelo de Farias (PNCC) for microscope support, and\u0026nbsp;Sujeong Kwon (KARA) for assistance with the Orbitrap Eclipse Tribrid MS analysis.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eSupplementary data\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eSupplementary Data\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConflict of Interest\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNone declared.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis work was supported by the National Research Foundation of Korea (2023R1A2C1005945 to H.S.P., RS-2024-00344154 to J.Y.K) and PNCC (#160183).\u003c/p\u003e\u003cp\u003e\u003cstrong\u003eData availability\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe Cryo-EM map of the LARS1:IARS1 complex has been deposited in the Electron Microscopy DataBank (EMDB) under accession numbers EMD-65488, and the coordinates have been deposited in the RCSB Protein DataBank under accession code 9W01. They are publicly available as of the date of publication.\u003c/p\u003e\n\u003cp\u003eThe mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE\u003csup\u003e71\u003c/sup\u003e partner repository with the dataset identifier PXD066801 and 10.6019/PXD066801\u0026nbsp;\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eIbba, M., Curnow, A. W. \u0026amp; S\u0026ouml;ll, D. Aminoacyl-tRNA synthesis: Divergent routes to a common goal. \u003cem\u003eTrends Biochem Sci\u003c/em\u003e \u003cstrong\u003e22\u003c/strong\u003e, 39\u0026ndash;42 (1997).\u003c/li\u003e\n\u003cli\u003eSchimmel, P. R. \u0026amp; S\u0026ouml;ll, D. Aminoacyl-tRNA synthetases: general features and recognition of transfer RNAs. \u003cem\u003eAnnu Rev Biochem\u003c/em\u003e \u003cstrong\u003e48\u003c/strong\u003e, 601\u0026ndash;648 (1979).\u003c/li\u003e\n\u003cli\u003eIbba, M. \u0026amp; Soll, D. 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In-gel digestion for mass spectrometric characterization of proteins and proteomes. \u003cem\u003eNat Protoc\u003c/em\u003e \u003cstrong\u003e1\u003c/strong\u003e, 2853\u0026ndash;2860 (2007).\u003c/li\u003e\n\u003cli\u003ePerez-Riverol, Y. \u003cem\u003eet al.\u003c/em\u003e The PRIDE database at 20 years: 2025 update. \u003cem\u003eNucleic Acids Res\u003c/em\u003e \u003cstrong\u003e53\u003c/strong\u003e, D543\u0026ndash;D553 (2025).\u003c/li\u003e\n\u003cli\u003eYariv, B. \u003cem\u003eet al.\u003c/em\u003e Using evolutionary data to make sense of macromolecules with a \u0026ldquo;face-lifted\u0026rdquo; ConSurf. \u003cem\u003eProtein Science\u003c/em\u003e \u003cstrong\u003e32\u003c/strong\u003e, e4582 (2023).\u003c/li\u003e\n\u003c/ol\u003e"},{"header":"Table","content":"\u003cp\u003e\u003cimg 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nv/ajTfopZ9d69ry7SpxS6LMkWvNiuVa5u4n0vIFC7TREUsVvyyHV3679prrsFoycaIg4Kz69Svubn4ATsudmFoNHKT2Pr4OnvKdgZiZ5KlesxZa4dr+bNfENjrzLHEGbGZq2KatDzGl/LbtVNCufbguWrVas1yzKqwgyBJ3W6jil5GTo/z27bV4wgTNc41+oz/DjS6Ii73MYifKjNxc9Tj+C+py1DEadOHFata7j1Y6gS5xZtXan2kH4HLEkS6o8lw+OZn5GEod+GV+rOhCJQ4XqXzcJSgSfi+VlaUS72u6C87ChfPVydso6NgRXuPly/z5ZKlB69bKzM5SPWc4TVxYW0ba75vDulgLx4xWExcOHQ89XO0POEhNXaDMcctmgwueMklZjRoHnGnvhJzlzxbhwzPyW+F/Zm6ecjx/rgu2eV6v2N2BpDIfa5kPNTu/QK333S+03cDhvsH9ykXuhpn51ptK12+grm7pdT7sCDXu3EVlrhSpyo8xNOneXe2cUTds1VpLXSFY7XCb7AJ77rtvq9BpYcXMYmFJxwAAEABJREFU6WJemfXqKbdxk3CeSqdV4vAzM5U5rDIdPzocdJDauhKU3bixCp0hbnCXB+2tcuvgI6ebeY5PqxwXVnqb5swrIztbrX3sHQ46RNQpdcbdzvGHBA2sc/pa5UKD8Sx2IT3ZBdsK15gXuyVQ5IycqWR4v21ceWrr9NGoa1cVOVxTDv/89h2VkZXp8+4c8JeypNLSMp9DU3U+4ih1OPgQNe3dWwtc4QHm9Zo2C0rXLBfGa52plzoDLXa3mnyOUplauBBo5zRe359zgbtVV7qSM8dhtMHhXez0U+IuOsmUcvrv5nBv5XSb06jABWo7MadWgwYJnCsCLs5DVjpc549+X5Of/a/Wu4W5wumozGFar2lzWcrU1AXuRp9n4dxZwSqY7PNf7lbOYlceCl0BKZMJl3c75wFtnO9k5+cr7c9lniuL8z8YHZ5FqwEDlUqn9Wl/e1r9HRAEpkWORB899W8nwp7a97yvq9WAATJLpmZ+7inlwPLMdFa2ynxxrcx9c2VeJCQnuuKSUjV3pOkw7CBnBkep6+eOV5b72b3Ipv8pR2T5A9yUkZz4wy1zpOIhJ1kcC9q3U8oZ4wrXNEqdQZe6BOd8DhqLa3VmPi5PtGtmynKkb7nPgGCWznbGkO9MJ79NW4W63n5GTk6YSyojU9QpdiTe6O2sc0FR7Iizxo9lzgxS2Vnq++UzdPQvfqWjf/lrcWztCMOYSBm5ucpv106rnWAh3gZt2yu7QUP5aLi9WTJLyRzR0plZkplSrt2STApa9wdu0nLd/6xz1OXIox083JEXNQFb+eV6t3w+cPfNah9fr5NOUY8Tvqi0Mwl5e6r4LXLtfZSb/vVbtNTAr52rFn37qZRn5IQNAVrKx+Hl6ates+bi2aQkmT/XtD8XM3PGkK2UX6vi549FxU7AZf58y0fjg6m4Fw6e3861sP7nft2Jua1mu5B6+6YbfV3jNecLYfShGH9oy2SchmTmLTqDLHZhn8rMEjAyHyPPqCyMuzSUS6XT4Z6o69UDjtCY/OfHxa5df+RadFO3dAadd0FweXoxv1n+n3ZTzvi4YkQkOQ6mnQnKTGmfOzABr7XFX5m4X36rLMC0Yeu2au+Cof0BB6urC9eCTp39WdFykspLb/rr2ZZKK+V9ST7vkCTnet5eqeNSe3VwZtXBGX5XV1bALZceMrNQx8zCeZiLwyPtOCT/+fS9fkkQOGjiKBmdHIc6H3ucMrKyfExSKu39VpT3RryW/2c83qafaVOekl+Z1/MCqZTf8uS0UuJWQJnTz7iHH3SteoF6nniS+p/1NR++l/P/SU3zsmYWXEjv/P5m7ztD+5x5lgvhA1VasaZF6wL+ARbyaZqAi5kfUxnyTj3T/zuPycjJVcv+A4IQ7uL8pO0hhynLhe3HXTosHQdLHcfhPe3d4u7sAqzr509QdoMG3kipLJVSAq9mPXpq0EWXqvk+/bXMFbiRf/idxrmbaaMrYKpjv9R2z2cTNMtU4sROvfy27YLEX+0LSRA/eRBe4aLFWuhSdqlL5IVuotVr0VzZ+QUO6FBCuY0aqV6j8utGXbpo/YoVjgzzlZGVTYFqyTzH+ONH/q91aT/DNcrVbgZznSQebEZBvj568nE39x7VZCf4kbf9URPdRF7tWrE5JYR2/JjUaeUMe737exeP+zBokhnO/Ou3aiXGPNPdXcxhvpuLpa5JtHM3UTM36Q/50XU66Lqf6QBfhwhjdqRf6JrI1Gee1hQ3R6d6muF1HWtDN+awK2jfQRkuELIzM9SwZUuBcOFmlT9ZLpwatm6jRe4aWoq24tpVoZvoMgUBBaOt55oWhIYmQx/APtOtjo0+jjUIqCWLVeJad3bDhsp2LXa1a1plrl2VOrEk3YHM6VRKeU2aBnfQOjeD5Z3AxBp36qJi14Rnuxa8wE34d9yfO9m1p1wv29BhQ79L3Jqb8aq7TVx782pKOyPJyM4OazRLw3P/UGknYMkn7/+NQn6+0tcOih2W3dxdt6+vP2XUyxPWV6kLbTPz4mXK9GdQIrkLcanWuCbpHMSvygQTbtq9h5YDlwnjgq96qRNos249nODre12FXsKJFM5pUhU/H4Y2rt/gICtTvgv8De4CWe3CGfhRxHsPdTgnmVm4TqUz1KJ3P21wq2+ua6wLXDssdIvWUmmKbZbKa1jIS2VmqUGr1mI8Ba7xZtWv526jSTKD7LwMAxJ3Pfm5d+cQ8gy/ZeZ//JT/nJEyc3Mcd1qJ80aduyrluLTMYW2hPcn8n09O4QeOe5uhQT+maM/z8ty6yEUJ8esCF0hr3dJY6zBOZWZSO1RN/pifkPygbF9jK3Ilq9DpqIhnTqYnS6V8vWSZ5riLbolbP8snT1Zjn2va2ytyTT27Yb4ysrO10N1WGeaM2Mfg1cR4/IrTcuXBn3+uj63McXi1u+5S3m6pn4f+ffxl1PNj+bWfhJplYczcatCmjTKdvrJcmWzUqVNwV691Wsh0ayLTcSzl5Ve7JwI6yXVvRqkrigU+znVuJa12+sryumXekHmLHIHjarfwC92CaucutP0uuUz53u5K9whs3JsFQWZunnLy82WOdACwmTPEKc4cprsPNa95C5X4Q4OgMnJyleUMaPm0KRrtiz+lrvl3POxIR6SG5al+AzVw4uj6uRMcgZb6wuc9KvTFqHau4WQ4A1CVn/k1+dkgryOHX3q9ZZrhjLbQNV6uk1TPNVc05fx2HXwB7nVNdX9qjguc7q4RN/Q+zZEzy9uBaW2q06Kl8l1bh/m0HjQoZNf3vG5fOFFlxaVhIWyu+2s7uCuirZvLqXRaOQUFym3UWFmuRYDwmXn13LU0UR898x9N9gWpj9xsn+l+8DJJmY6YGc7cKd/QmU+2M9P6Lgiy8xsGeKbSmzOTrHr11O6QQ5XKyvKF3LvcvzxGaYd9Ojs3IHoHd8lAcBOeeFwF7dsr5Qhc7IjJ3Fu6336Ra/rLnFG2ddfIBveDjnWtrMQ1tDwXHmtdgPqQwv/G7l5p4X7gub4YN9PXABhbyuFb7O6hNrgG9j9AH/nzneBCNNufO3PP9vl2PvoYZ86LRbsbVq5SOjPTaaZMjLvzkUdqnfv7Jzscil1ZSPvc01nZzghcg/dxpjOzJDMtdCH34X33auKTTyjXx9XGCS0jO0c5+flBWObkF6jlwH210l0gM9w3bxkZymmYL0ul1d7dDwXulpnoaxhjffGuvgumzj6mjJwcZbgwTHs/KS9nPpdsf9aZnmcpsEjetamRE3Nzx93J7j6Y6v7lvOYtK3C3ROnsHId1rlJpfyZmyvLxZ/rzMK/f8dBD1bL/QE12mMz2xeYMf+bZDo+U96OKn5mVjyE7W96ZMnxMHd2NlOf0MdYXZWe4v72Fa5f5jm8ONGXVb+B91POipsy8XFlOrtI+V8ac7TTEHEjZlPP+Mv1+xyOPUqbPa8z9/9IcX59p4+6Ses2bK5WR6fkNlM5yGEvKcpxj/tS3dFppn0c6J1v5rpCgAa9yATjGn8EGx5F2bqUxD+rmeNupdFr8Mry/lCfaaODKSaMu3bzPd33tZEq55PJCMM1sFxJrnIbHOa6VOQy6uoWR8nl0OPhQbXBhO97XA1M+vlynz41uUadSaR9rQ2U4vL0JNWjZUh1cM1/ibsePnnlG9ZxGMh32Ra6MMIbcfJ59Sowj7XDK8GdMPdrMdjil0ik1cqbe5djPa7ErBmN8Xplepj08xY/Ne/RSow4dNf2Vl1TqfKqb0/YGd5uO8YXitS4EOx58sFJZmcrIylY2zzSdIX8oSmVmaoUrUeMcz8Y++ICKXXh08OdJGdWxX2p75gNy9vziiTrkez8IgKrfoqUGnH2eDvjmtzXw7HO179cv1H4XXiKA391N1QOv/oH6f/Vr2v+yKzTsym+qRb99lOlEsf8VV2ropZcHJG3jTHWo3+d6sPugW3oZHrSq/EDidu7nHPqNK5RT0Ej8ClyT2d/7btF3Hy43S03dnBtwznk65IfX6rCf/FSDvb+27ofMyMlxra6vDrrm/8J4KldKOWI28cXHes6UyE+l02q5zwDtd/ElGur9DrnkG+py7OeU4W1wv3Jq5EzpoO9+X4dfd32l9HMNvfxKxyXToPMvUJ8vna4sR7Chl12uIZ5fzwmix+e/oMN/fJ0jfZPKzclSKTVzv/fQb1ypYZdfpQFnna2Drv6+ep98SrjX68STdeC3v6f93K3R1xfSh33rO+6i66VsJ4j9LrhYB3/nanU48GB1cZP/0B/8SIMvulj7fPUsHfKDH7rJfdCmvvLcKtv33PN18Pe+r4FfO0cDzjtfh1zzQzVwgs91zayXu5QOuOpb4RkOOOscNXQhZj62toOHatgV39QQf95djj5WCFeYGg13O+ZzOuj7P9C+3la/07+iQ31+7dzH3bL/QB38fz8OcG/icN7Xxz700m9oiGtZ+/mYm/XqrQbO0A+/9mfq6m1mOvPu+2Wvf82P1MNdKQPOOMvn/F2lM9JerrW74U7XUMcd8KvfV84sH5uZ+jiMBnl7eU2aiDaGOrz7nPplZbggVcWvfouWGgDufuvbGnj2OZtwN8sZT0cXwEMuv2pTe/3P+KqXOU85+QWq589soOPVgd/6ngY4bg/z+sOu/JboSxW/LGe++zotdPcxpzMyZGYqcAY18GtnOxwv1/7+TLv4/LKcKafS6ZDX70tfCWPtPfwUDfvu1cp3K7vnCSeG+eY6vufk52tfx6EB/ozM22ziApznNtTxen/HzQ6HHq5MZ3Yt+vXTwf78WjgdpdJpx7sLQ/s5+fmq37KFDv3Rdep06GHKyM52/DhIQ30s+zt8Bvl4m/XsLR+s2g72Z3vVt5Xfrr349fzicA08/+vKdVzJLSjQEO/zAKfnZj17ivLi51p0lsOuh7tXgPdQf67N/Hlyq72v4Rzg+Dnk4kvV59QvaZifdzjwIDXq0kVH/PQX6nDIoaKdzNw89T3tyzrEcWeQu5r3cXgd4bjAuHgmB3/vGuXk54dxDHS3Yp9TThW/Rr6Qvr8/r2ZOLymHTWcXksxp/8uv0CDHgwLnE5Rr4ms2w5xmhvg4gF/bwUO0v9Mh8NvPeU+Lfv19GCm1cz6zv+N2A1+jol59x8l+jl9DHdcGX3SJ4+s31N7nlM4qF7aUqSsptV0TMVNW/frKdQZhqZQDzcJ1XtNmynEECfcaNRL3shwpcv08u0FD1eO+P0BLp+WVlAtie3kzUyqdVrYzr3rNmvkxX6GMtvwD0alL+5RIZ2Yq1zXydHY2l5slM1OmM5I8ZwZ5rlkwjlQ6HcrwAPN8cS4ju7ze8mnT9NJPf6JlM6ap7bADw5hCQf9DX8yLOeT6fOjTs6v9Jz/X4ZLXtKkqp1yfK4Vz8vOV3aCBzMyRuUDkm8MQZpDnY7R0+dgomyTul8OmuTjmeFuMRf7LyMlRntfL9T45z3U4ZGSXzye06ePgGObq5fIcBuXPpHHQPr2JTf8DnLx8jj8TyjB+5iP/0W43VGsAABAASURBVDZ1yaM9M/NcKeUEl+Pl87wefeTk5CqdLp8D18CXMWXl1QvwoJ2M7Gwfc9PAhCyV2oQ79Xj2wMbzUt4u/TEm+S8zNy/Uz8l3+Dme0KYYg6cM7zOPufkYMnNzPdu8hrzdBsrxsVk6HfJyHG4J7FXxMzMvV1/hufp94Jrrz5dx0SfnjIXiAfbeP/e4Zmx53md2w/zyZ0k974t7JMrl5BeE9n0AZPnBHB/LYZHrzwwYVdwIY03GxziAXcrhwHmuP1dzuJBy8sthQD2ueR7AjjLpzEyyA2zzHCYZ2eW4kJPvYwQW3kYqnRFgyfwoTB85+fmqF+DfULRJfkZOjnJ9TtznmnEwH0unJbOAx3k+f8qp4peRmaFsrwct5jm9Z/uzStpL+9hyfR55joPUyXF4A0Py87wdnp0qftynXK73n5lXL4yXPMrkOtzM50HKyc93+DYItWiH8glMuc7xOfNsGbuZiV+o5/m0n5GdLfP5ME7KZfuz5D7lQl/ef8qfAddmFoRsno+1nsMqx9tIVdxTHful6th8PtF06rlJ3efkUzXEJX7rfQdJ/uD1iX97b4VsJ8rervm38HUWiGvvhcTeOXOYYutBQ9TtiycJpr13QqFuzLqaIFjnPr1FU6Zo8dSpdT6tXLhQuS1bqcw1pmWzZtX5+e7sZ7rCF9MyXcvbsG69lrh1tbPbj+3t2TS4ZPp0FbnfnPWHpTNnRvrZmTzTF6VX+GL3al/D2VlplfP2NWvWuCeX1cvNBVg1QTDrvZH67y9+qid/dm1MEQYRByIORByohAO7jS9e/zP94BuXafjw4TstnXjiibriiiu0fPnyzaWAX1UTBHnuQ2/Zp5/aubkf08AIh4gHEQciDnwGODBAhx59tL70pS/t1HTssccqx9d0nPdv9r+aIGi7zz46+qpv6ShfVY/pygiHiAcRByIO7H4c+MYV+vL5X9dFF12009Ill1wShEpeXt5mQoCLaoLAzMTCX0zpCIf0XgqDOO+I+581DqScNZvBo3dL8t52Sz+xkwiBCIEIgQiBPRQC2xQEfIm3zhcXVs+ZE8JAFK9bJ1923unT2bh+vehnpzccG4wQ2E0QKN6wQRtWrdwqfWxYvTqEUykr+/itjY1r1ohQKaucvkiFC+aryPO8kTDq0pJirV22THwJvHr+fG1ct9bJ7+P6tLV+1SqVFBWF8pX/lBYXe3/LxTHJZ4xrly5VWWlpeZaPZcPKlaF9+icVLpyvYqfH8gIKoU3WLF4cyqxZtEi0kdzjyNfCzI3z3Z2KCgsr4LW7e65b/W1TEMx7b4RG3nm7Rv/jLr3/t7/og3vv0aoqMX52BkgWjx+nMQ/ctzOa2s42YrEIgZ0LgaVTpoQwI1UZJb2UlZRo3KMPa9Q/7w7MOeQ5M5773ki9+8c/aPTdfw1p5J+hNS+zclVg4HPefVfv//VOjfnH3eH+2AcfCOGhqV/qbS75aJLev+tOrZpbvgcA+Ula6wx//L8f26Rgwfznj3pfb/7ut1o+Y0YoVupjmPTM0xpx6+81+u936QNPI+64Te///W8qWrtWCIQpzz8beABjoK8Jjz+qjX6vzIXIGhcMY+67R9Ne/F9ob3f+YT6z3n5Ts999e3d2Wyf7qlEQlLiWMfutN0Mwtn0vuFiDzrtAeU2ahlDMPASQZPmM6VoxY0ZADCC0zq2H9a5hrJw9O8SdoVzhwgUBUTkvdS1l7dIlWudazrKpU7RuRcWrTCg5jli0QZlV8+Zp6ZTJQuKTF1OEwJ4PAUfiChyuOtY1SxZr9dy54iMsaENJOfcDtx4yVIMuvFj7XXypBnztXJU5c14xs5ymlkycoLZDh2pfv9/f7+U0bryJlua5EJn+0ota5zQnlfuTi90qoa+y0P7m4ylev04r58xWnrcx8/VXtennxToeeXTof9BFl6r/mWf7WOdopY9hzeJFWjppknqddIr2vegS9T39jGB9rHbrZLUrhB+5EFnl8yor9UY2NVh+An9Y4byhcNHC8gz/W+ZzW+PCY6nT9hq3MsrKSoWQLMQSKlytlbNmBZqnHFZQodctKy0Rnog1Ppa1S5a4EJseBJQ3JzHPir5LnF+t8PorZs4MQpT+13j9Uuc5pNXeR4nDJ9SLfzaDQI2CIJWZoZyCxiEoHEDObdJE3T9/vNrsN0Qb3Mwd5VrDG7+9UW/c8htNfOrJgCDTX3nRrYZ/auSf/qB3br9VM998XSNuu1Vv3fwbLRo3zuutCjsAjbjzNr11y01690+3asWsmZLJfxbM0Omvvqw3b74x1Hnf+1jrmo3fjP8jBGotBGa/9ZYKOnVS0+49tOSjiSpx5pRMJp2VreyG+QrhUJzminANuYBIZWQos149FS5YEMrXa95c3Y45NsRsIixCrjP0rsccp/x27Z0hlrt6Cp3xTXn+f4GOJIiKpPBb5wKDtjsddkQIJV/oClpyl2B9OQ19DA0bimrrcXGlUsrMy1MqK8uVvenOc8ujtvY55TTlt2unjOwcdTjoYLUauK9K3TpRpV+pM/wPH3pAr//mV55+rYVjP1SZ580d8W6wSN50fvD2H24J+2EUubtrzP33acz99+qN3/5a4x5+UFP/96zzgN/o/Tvv0Gqf/8q5s8NOZyP+fJtev/FXYYe6jbipHU7yVOZtj3vkIb3p9d+46VfexkNav3JF4Esr58zSQu9/ApZMFASVntLHpzUKArOU+p56mlLptN7721/1vx9crYlP/lvFG9a7FrJMWQ0a6KBvfVfdjv2clkye5PkbnNGvVjorW/t/+3vKqtdA019+WYMuuERt9z9Ac0a+6whbpvUrVqjt4P113E2/c4RqH7ZXBHHkWLnWkXXhmDEa6NrPMb+6SfVbtNAcN/1KqyDax1OIZxECezYENrimu3jSBLes91N+m3ZaNWdu2JWPUcPAPnrq33rq8kv1xKUX6sXrfqycRo3UzAUGTLjLkUcJXfs9dw89/6MfaNLTT2mja/bUbdK1mxq0bClzhh0KeWZ+27bqd9qXlM7K8qvN/y+bPk05zugbuUAqaF9Od2Vo1F55rCtc9P/viy/Qa7++QW0GDwkb5uQ1aapew0/WKtf63/njrXrp+us08/XXxC+vaVM17kI47Eyna3I+Tgs/HON15ujQH/5Y/b50utP88pDmuhXT4/gTdMLv/6SOhx2umW+8po0u+FbPn6sGrdvowG9fHTwBq1ygHXLNj1SvVWu3AGZIssBbehz/BR1x3c9UuHCh5rzzjmebLJ1y/vORVrhCOeyqb+uwH12n5dOn+trLvDC+Dx94wJXPe9Xx4EPD/BV/1SBQoyDYsHqVlk6bqt5uFhJ1kMiCxb6INOW551wbyFaxI+Sk/zzt0n6sWwMbQ+PpdIbQeuo5kjRo3VqtBgxQwzZtwkNOuYYD3tX3h9u8T9+ArG33Hxbqlrhw8WcdzMLV8+dp4tNP6t3b/qglkyZqvbuaiB8eOoh/IgRqEwQc4RePHx/8+uN9jWDcow85k5qmub72xjTMTO0POkQHfff7gWE27d5THV3LTjsjL3IGudZdqL1OPClEfB18wUWBac587bVqGjhtkcxSSjkNcl45lbgmPMf96fNGjgg7662YOUOL3UJf565Z6nT+/Ak66DvfV/fjPq9mPXsG5S7l9Frobhxob7/zL9ABV16lfdw1tMjX82a9+Ubl5qudF7q10diFRP0WLdVmv8FuORyijevWBwujSbcesnRazXv3VUZ2jjb6eoOl0mGToDz3OtR3vtHl8COVU1Cg+i1biXEgsAo6dlKzXn1Ur2kzdTj4EPdULHC3UqnMTKudZ5DGPfRgCN++YU2h1rjrCV60wRXP3MZN1bRnL8XfliFQoyAoLdoYNnWZx6YS/sDy27YLD2eDm40LPhjtSLlWPb94otofcIAyc3M29WBm4dxpQJYq76LMfYEh0/9Qf437TMu8wDJfJ0hlpJXKKNcq0tlZynWTt61rJF2OOlqtBuzrgqStI3da8RchUCMEPuObJlOpuyjA6zKOnja6+2KRM9zORxylbsd9LrhWux59jGa/85aKnNFbKqU8Z2yNu3RRJy/Tsv8AzfZ1uY2ucMEgpz73Xy0YM1qZubmBDrJdo9+weqVr4KVbn63T1eY3y7TYLZIy96X3cqWu61HHOEM/Uxn167miNUll/q+hM9+mPXqo+wknuBXeUhOf+HdwL611v/x4d6ksmzZN2Q3zfQxtlOG0Dv1u3sfmV9kN8kVd5rDa1xPmuSWQzsp0pW+D1ri2T+nVzrxLiFXkHgSfkGT+18dultKmn19z7reCF2Ktry8CtyWuIGY7LCxlokhWvfqq16y586ID1dmFSHMEhrvSlrki28AV0ZKiDWEnQtqKqToEKkG8+s0cZ8idDz8qvO3wv//7vv73w2s07/331G7YgY4QbbXCF2XevOW3QViscVONh1rqLpwyX9yhtVL3g5LCeUmpODd/osVOHOPcD/js977l5t3bauU+xpRrBKWOFHneZ6NOnTXFfYQf3HtP0Jyy69dXKiND8RchsEdDwDnSHF8Te97p5Nmrv6Pnf3SNxj/2iIrXrhFx9Vu5UkPq5IyqbGOJFrqClXKCMK8n/6UzM9XWF45hWlOffy6EhG7Rr78m/PsxPedu2ed/9H/Bt99m8FCl0h/TQ9rbgJl7E8EdM877LHElrszbLXUaLHMBMOPFF9Xa6ayNa+et/NhqwEC19rTEtfuSNWtV5kKL+ll59dTtmON80Xa6pr/6SnCtNGrfUe/c9gc9+/3v6OXrf6qiwjXq7IvLlCel/E/KhYkfNv1v5QKNMbzw4//T6zf9Wmt8nS+noJEKvK1R/7hb//3ONzX24QfUpGs3ZTl9U1Y+XhcFvh6ycZNALQ3jd6Hnc1zn1tGIP9+uF7xNNq2BD5U6v4FvtOjXTzn5BRrvQuvDB+8PL7QUuxU0f/T76v65z6uLC9/JzlPWuQWk+KsGAZ5htcwkw8yc6Q8TG4D0OflU9Tn5FA299HK16NMnLFgNvvhS9TnpZA2+8BINvuRSZebkqOOhh6uZm3y00dHNtzau2XPeondvdYEAyqT6LVupjy849XLf49CLL1OzHj3VuGtXf2DHKzM3T71OHC42ZOE46Lyvq6UjrHwsir8IgT0YAg19AXWfr35NvZ1Oejlu9zjhi4Lhdjn2OGU3aKDkl5WXp4Fnn6N6rVqpsa8FNN+nv7AMuJ+Tn6+eJ56s/PYdPC+tDu42Gnzhpert7fX90pc02N1DTbt1V0IP6awstT/0MNV3F4z8l5NfoJb9ByjlVnZuQYEz7KOU5X23dau97dBhXuLj/60HDlJ7p9F2Bx+sJrRZcSvX3TP7nneRGrRuJdrv5X0P9jH08nHtc8aZGnzRJcrzdQxV/Fp4f23cxVtxGQ6Zebna7+sXqN+Xv6JB53/dhcuxQtB1cSt/kNN0z+EnafDXLxI8ItPhMeBr5yi3cRNlZOeox3HHq37Tpkql076WOCTsV1a5AAAQAElEQVTwh9KSYjXq3EX7nHGG9vnKGTrQ1ybzfH6tvO+W++yjzJxcDTrv/HAP+A+78ltq4jylx+eOV0GHjr4+M1jdjj7WwZZS/FWHwDahYqmU8n0Bqt2wA9TWESnPkSRpBkC3P/BgZ+Ld1Mx9mzzQgvbtVc8fImU4b+jrAZznuflb0LGjC/1Slfm/em62dfC69XwxWP7LbdQoPDg/DcjQok+/QAT5bdv5wzOyK1I8RAjsmRDIyc9Xu/0PUAfH6w4HHqT2ww5Uc18La+Jar6XTHw/aTI2cqZFfr1mz8gVfz0sKNHABAYOzlIkEHbU/4EBnikOV16RpUiwcU24pN/a2surXD9ccm7hv3pxuM1wxa9yps7Kc0bYdsr+y3ZUSClX8QUA0c795UxdGeRU0W3FL9Nm8Vx/v31mESU27d/d5HeSCbV9lV/SVlG3gNJ7frn1yuemYVb+B2rqFQztJZsrH27RHT3V0AdfIx8Y4024JMd/M3Fxxv0m3bsrMqxf6buAuq9xGjVTmVo2Zw61jZ19zGCLmJr9u0LKV6jdvEZrP9Dqt991P7ZxP5fhcgVUjhw1tptJpNfO55uTnh7Lxz+YQ8Ke8ecauvsqqV09t9xsikGRX9xXbjxCIEKgbEIDZt3OrI5VO140J7WGz2O2CIDM3z7WkPkFL2cNgEYcTIRAhsIdBIBlOXuPGatFvH1lqt7OsZAh1+lgNqmuWL9e8ceM0b/z4XZbmT5i4y9releOObe86nNilsHV8Xjl/3k4n5FJfqGRnrl069l1Ih7Vq3BMmaL6nWjXmT/PsfK5rV67c6Ti7tQarCQJ2KHviZ9fq6V9eH1OEQZ3AgSevv06jn3pyazSww/lFa9fq7X/9U0/9/Kd1Ak6R5vccnvfMr36peWPH7jBuftKK1QRBC184OsBX8Pc/8yzFFGGwW3BgF+PasLPOVhdfxP2kxLGt8hnZ2ep91NEa9tWvRVrZxc+wLuDhJ5rDV85Q006dtoWCO+1+NUHQqF079T32OPU5+piYIgzqBA6Az2369NlpRJM0lJGVpY77DVafY46tE3CKNL/n8LzeznvyW7VKUG2XH6sJAjNTKp2OKcKgTuHArlpkpN1UOtJLKh1hkErvPBiAV2am3fVL7a6OpNhThECEQIRAhMCeCIEaBUFZaalmvPqKPnzwPo198P7y9NADWjDmA61fsTyEmC5ctGizeS2ZNDHE9CjZWKSZr79aXsfrjn/sEc19770Qv4QKq+fPD5/fh3a9zakv/E/rd+MqOWOIKUJgZ0JgzeLFmvD4o5twHtye/N//aMXMGZr5xutaU5VWPoJWJvv9mRr/6MPl9ZwWpvzvOa1Zsrja0EqKNmjuyBEa62XmvPO2SotLVLxhg2a9+YbTaDl9TnvpBRGLJ6m8esF8LfxwjMqclks2btSCD0Zr7MMPhjolXldlZVo1d24IzTDpqSdUuHCByEvqx+PeAYEaBUGpI89cR7hVc+ZoQ+FqbVi9KqSSoqLAtMc/8pAmPf2kuAZca5csEXFE5juylRRt1Jw339TKObO03usWErzqkQc08YnHHc/KQrTAue++E9qj3bkj3tWHD9wX7tFWTBECtQ0CaxzH5496P2y2BE6vd3opWrPGr1c4o69CK8uWavQ//q75Y0ZrxYwZmv/+e+U0FgI6jtKIP/5BhVUExwwXJhOefNyFxCJn5g9o+isvaf2KFZrtNLp++XKtX71SRWvXCKYP7Oh76jP/0dx33w55Sz/6yBn+I0EgjX/8Ea//sta48BrzwL1a4cJqyWTuP6b1O0Eho/+Yag8EahQEKpNSviDWa/jJGnTeBRp0/oXi2MYXyMpck8humK+Njnhrly4VvwVuKeQ1biz8W/L7ZSb1Ofk07ed1h1x4ifbztNC1k+XTpqqkuFhNuvdQ0ubAc84PuyIVLlyo+IsQqI0QgCaa9e6jgWefF/B6P6eXPqecptxGBcpq0FAb161zJl6u6S9wZSmnUSOl0mmVlGwMH0sNcjqBHoZd8U3ltWiuaS88twkMJa7Nz3eLus9Jpzo9Xah9z7swMPH1bpnnejv9zzxLA886Vz0+/wVlN2gg6G/R+HFaucA1/IzyAHUrZs9U2yH7h1hBA848W4vGfajlM6apXpOmIZ7Yvmefq1QqtWmMmzqPJ3UeAjUKAufjAgGXuLsHxF3wwajgFlrnyOeYJsJFNGzTVqvmz1Xx+vVaOnVKiFZoRs1y2CXaCVeNOnZSo+7dg6ZjZgHhaHO+tzvv/RHKbdo0EA1lY4oQqI0QWOsunYUffuAumFECr5dNm+bu0GIR76dBm9YqdFdNsbtklk6erMadu/gUyyClEG3TL8L/dFaWOh95jApdW6csmetc2SrxeqvmzglW95KJ49XlqKO1ce1abVi5wi2EB/XBv/4Z3EAlLjSWzZju/Y9WhwMPVGZ2Dk2EmDyr5s3TvPdGasGHo9WgVWvVa9ZCG73duSPf9bwxktNlVr3yuEWKv70GAjUKAqBQ4m6gOW56TnUf/tTn/6dp7oMsXFCutZe666hJ125a7ci1YtasoE3Ua9FSlZk/bVROmbl5AqHNUsE3OfWF513z+Z+br++IYFbkVy4fz/dgCMShbQYB86sVM2dq+ksvKtCK0wuKDowZWmncuatgxCtnzZS8cH1nxGWlLghU/ZeZm+t0UhTCKS9wK5p21y5bEhh/k27dVeiW80TcrCUlzsybieByec2aafqrL2uhW+bTnK46HnSw8lzblzN3esjIzXFanavpL7/oguoD5bnilVkvT6W+9jDtxRfCOobckqdvyse090CgRkEAioIUvU86RYMqzNZ9zzlPjbt0DlpMWWmJ6rdsFXyK80aNdFdPd2U4Apc5Mm0JhGUuOArdVM1qUF/Ubd6rd3m7buYOvfQKrV22XEvc+thS3ZgXIbCnQwB6ad63n7uGztUgdwuRuh5znDKys+UqvxpU0Mpc18ib9ughaEtU2sLEVvm6HNkzfB1g5J13aMYbr6pBmzZuKRylTocdrp4nDndhsED1WrZUrxNPVsdDD1Xnw49UXqPGmul1FrrradLTT+lDFobfelOT/vNU2PCms5cZ7C7aoZddrnmjRrlQeEkNWrfR4Isv1aBzv650draWTpksbYWGGVNMdQ8CNQqCMF0zpTIylMqsSH5eVlKqMv8n/+EeSnve4vHjhcbDPc/e9L/Yzc6iNYUqKizU1Bef19qlS0K5UCBlm9qV/9hsomjdWj+L/yMEaicEUumUKtMLs0ABgrGW00qmFk0YpyZduov8hI44D3TitLLCreuJTz2p9gccqCEXX6YTfv9HHXDlt1yxT4U38qClhWPHhvozX39NE59+wpWxVSpctNAXnAvVpGdvtdl/mOo3b67cAl+fcK0/x4/F6zdo4/p1Mh9jibuPiv281Nfqipw2oWFzeiSPsSj+9ioI1CgIzKSMzEyNuf9evXnzbyvSTUG7QA5k+wJY2v2ZDV2jQNvJbdxICIyg6aRSyqlXXx/ce4/evOW3euv3NweTtecXTlSuL25l5eVp5fTpFW3+ViPuvF2NOnVWy77996oHECdbdyCQkZmppZMm6e0//qECr2/SiNv/pJVzZiurYcOwMUtDXyeAVvKaNFEqnRGsAlyii8aNDXQCrYy57x613GcftRm03ybgpNJpdTnyaM10xv/Gzb/RnLffVM8TvqhOhxym9ctX6K0/3KIxvkZQ0L6DOh9xpAb44vGAs872xeMT1OGAg0Lq5JbEArcC3rrlZn3oNN1+/wOCFVFaUqw3f3ezRt75Z6VzctTE3b0y29R3PKn7EEjVNMVUOq3uJ3xB/b78FfU+6eSKdIraDh4SzNxeXxguc4bfYp/+fu8UZWTnBF9l2yH7+3m2uh5fXreXm669TzpV+7rpyQYU9JnfoaP2+erXvN7J6jX8JPX70unq6ykzL5fbMUUI1DoINGzTVv2+cqb6OK4Hehl+irp//gS16NPP3TcnydxybtGvf7ifdgWqUadOajtkqJr26Kn+0AJ0Mvxk7XO6t3HKqcpwplwZCC369FX/r3xVvU8+VftdcJFa9h+gBq1aqe9pX1Zv76vf6Wc4Yz9is3oNXElr7fRqTsvNevbSgLO+5vVP0YCvnq1Ohx9RXv/UL6nPKaepL0dvJ9cVtcr9xvO6D4EaBYHMVOAMu0XffmruSJik/HbtlekafX779l7ElJOf72sFLWUuFLIbNAiLV6l02ut2EHVb9O2rZr16BUsgASnlmvfuE9pt4e036dY9aEfJ/XiMEKhtEMisV8/xvLeaO743h178CN7n+aIsmrrZFmilaTPlFBQo0IKXp15Bhw7BWlCVH8y8QevWLlj6qoGvN4Tb3mZu48ZOZ32DJp+RkxOykz9ZPqb6zVsEOjWnz/pejz4auRBKZZS/Vprtlj39N+3ZU9luuXjhpHo87iUQqFkQ7CVAiNOMEIgQiBDYmyFQXRCUlYVFKBaMYiqNsCitGzCQ4/WuIPQybzfSSd3AkT3tOe4qnN0SHVQTBIumTtG7D96nkQ8/GFNdhcFeNq8RD96v6SPf3RL+f6o83oib9MpLGvHQA5FW9jKc2tX88f1HH9ZSvjf5VBi6/ZWrCYIl06bpg0cf0ciHXBDEFOFQB3Bg1CMPa8a7I7afKrazJF/kTn7lFb3/8EMRT+oAnuxJPG/0449ryYwZ24mJn75YNUHQpt8+OvyKb+roq74VU4RBncCBI6/8pvoce+ynp5YqLWTl5mrfU07VURFP6gSe7Ek877DLvqHWvftUwbgdutyuStUEQX6rVup20MHqeuBBMUUY1A0ccHxuzrvx20US218onZmpNn371Q0YRVzfo55jl2EHqEGzZtuPjJ+yZDVB8Cnbi9UjBCIEIgQiBGoZBKIgqGUPLA43QmBLEIh5EQKfBgI1CoKykhKN99XrN2++SW/97ubwGfqMV18OIaeL16/Xh/ff5/m/9XSzRtxxm2a99YZKiorCeJZMmqi3b/19uPf272/R+Mcf1fpVK8O9+CdCoC5CoHTjRs0dMULvON6D70Vr1mj+6Pe1cOyHKistDYHclk2bGsKubFi9OoAAepn6/HNaOXuWls+YrhF/vl0fPnif1lfaHKa0uFgzXn1ZK2fNCnXinwiBnQ2BGgVBaWmZlk+ZrHR2dvhyuGHLls7s39S0l19SsTP8pZMmKJMvFz2fLxKnPPesxj/2SBAGaxYt0rqlS0K9ei1aOBLP1IcP3C+IZWdPIrYXIbAnQGD+Bx9o0n+fVlaDBlqzZEnYUnLdsmWa/8HogPclztAXjRureSNHaOGHY8KQ1y5bqmVTp2jt0qUa8697xNe+hQsXafQ9/wgKF/lT/vesxj14vzZERSrALP7Z+RCoURDQnWXnqNuxx6nfl7+ivp66Hfd5rVm8SCUbNijl9wh8xb19vnKm9j3v61o+fbpWzZur0tISNevTN9TjfvfPn6C1ixZqo9ej3Zh2AAKxyh4NgUXj3ofBUQAAEABJREFUx6rtfkPU/8yzNPCsr6nELQTJVOpKUzHJrej1q1ap9eAhWvLRpGAlwOizGjQMdJPfoYMGfPVroe765cu0fNZMLfVyJV43t3mLUF7xFyGwCyCwTUFQuc+ysjKtmjNH6axsEffEbV2Rl5QpaNdeDVq1ckGxWGYpLR4/TuMefsg1owc18ckn1KxX77BTU1I+HiME6hIEMnJz3KWzXKWu+a9bsUIr3d2TysyUUmnX5ldpo7uKytzd2nbwUK11iwH3T+GCBarfoqVbEIvUsHUbp60sZTfMV3Z+vtavWK6W/Qeoy9HHKqdhw81orS7BLc7ls4dAjYLATAGBX/nF9Xri0ov05KUXatYbr6n1vvsqnVEesKrqFFLplMqcEMzMkXuxFk4YF+Kvr1myKJi6ZfhKq1aK1xECdQACHQ44OKwHPP3NK/Tun26Vc27hOs1t3Eir5s/VYtfuMwnW2K6d0tlZviYwQwiDBq1bh7JKVZCj045IZVKml8/IzvamyuSXdQBKcQrbCYHdWixVU29uAISIoL1OOlmDzr9A+55/oYZd9S017dZd5T+TmZWf+l80mNWu4eQ1a6ZS13w6HnyojvjRtTrix9fpkO/9QEvdF7ps2jQvGf9HCNQ9CKSzsoIrdPCFFwV6qd+ipbJ9DY1IoUsmTAhuHqKQZmRnq0m3Hr5O8IFKijYot1Fj5RQ01jpfTyhzRanYXUhFhWuU7WsNdQ9KcUZ7IgRqFAQMmM02mrtLp82g/dz/OVj13VdJvlwAlBVvDHufznn3Hc168w2Nufce5TVuovy27fx2SqvnzhX3ytPbjvRFysjJCdXjnwiBugaBlXNmh60fNzoTn/7yi3IiUEPX/gs6dAh7DxcuWqSCjh3Fr3nvPlo+dapkCm6fVgMGaOG4DzXtpRc16emnxK+h0xFHNweUcnqzcBH/RAjsfAjUKAgc95TdqJFSmZnVemaNIKN+/bD95ITHH9W0F/7nSN9evU8u31Ajy++tXjBfEx5/zNOjmvfeSPX84nDlt2lTra2YESFQFyDQtHsPNWzTTpOf+6+vB6wNm71k5dVTvabNwt7CBS4Ucl37Z67QQV6zZmrUvoMycnPVuEtXdTr0MM18/RWtmDldfU/7UhAQqvjluIIVlagKYMTDTodAjYKAV9kGfu0cYc5W7TnLfZcHXvVtHfur3+joX/xKh7kLiG0ocwoKxK/1oEE65oYb/d4Nnn6lQ77/f2o7eIgsneZ2TBECdQ4CWe4G6nPyKTrqZ7/QkEsuU4OWLcMcLZXS0Isu1YCzzla6QqmCDvb/xhVqN+xAmZlS6bQ6HXKYjvjJz3TQt7+nJi4YVPFL6LBpj54VOfEQIbBzIZDauc3F1iIEIgQiBCIEahsEoiDYHU8s9hEhECEQIbAHQyAKgj344cShRQhECEQI7A4IVBMEs0a/r//88nr998YbYoowqBM48J9f/UIfPvP0TqenorVr9e79/9Iz3n6kl8gvKnBgp9DMszf9WvPGjd3pOLu1BqsJgqLVheG1z+UzZoQPXuIxwqG248DqWbO0ZvGSrdHADueXlpRo9fyFIhhcbYdRHP+eReer58zRulWrdhg3P2nFaoKgVd++OviSy3ToZZfHFGFQJ3Dg4Mu+oZ5HHfVJaWOb5XlzbsDJJ+uQiCd1Ak/2JJ534IUXf7Y7lNVr1Fit+/QV26TF1CfCoXddgEFfFbTeud+vICVS6bSadOgYcaRO4MieheetevVWbn4+aLZbUjWLYLf0GjuJEIgQiBCIENhjIFCzICgr0/oVy7Vm0cLytGSxNq5bt9MHX+a+1vUrV6jQ+1m/YoXKSmsOr1XsYyhevz6ErCiq2OBjRwaFj3f9ypXeX+mOVN+hOkRrLSosVHGVcNzkr1sOrBcFOKxZtMhhvXaH+tiZlYDzxnU+DseFndlubCtCIEJgz4FAjYKgtKRUHz35pN77y5818q936v27/qpxDz+oVXPnbHUGxevXaemUydUY3dYqlBQVac6IdzT6nn+Efkb98++aP/p9lYRY7tVrlZWWauH4cVo0cYJW+CLg1Jde+ESMvLS42BfBp2vDqpVCCEzz+jC76j1J2gWZ7OMw843XtXz65sH3gMOERx/SyDtv13sO65F33akx99+r1fPm7YJRbF+TZc78l0yerIXjxqvU4b59tWKpCIEIgdoGgRoFAYygcMkidTzksLDZRt/TvhyY7oR/Px6Y6JYmu841+tnvvi209i3dr5q34MMxmvHqq0o29GgzaJCmv/yiVs6aWbVouGZMG1yLh4kXrSnU6vnzRV64uR1/0MTnvT9SaxYvDrFcOh58iHZnDJcyZ6iFbvlgFVQebplbRctnzlLnI48OsO5/+pkqcSE5/dWXRZ3KZXfXuZmpcefOatajh1KpGlFldw0p9hMhECGwCyCwbepOpVW/VSsRb6hRp07qfdIpSmdnOSN118XatRr70P168WfXCo2+aO0aTX/pJc167VVNe+Wl4NqY6ELjpeuv03t/+4sQElXnMMeFRvsDD1LbofuroENHtR2yv7oec5wycnMDA1wwZrTevPk3ev2mX4ewvXItVSaZJ/Ezn4LnLZs2VW/+7rd65Yafu4XxrtD8Kbtk0kSve2OwaNa7FbDSrYiZr7+usW7ZrJw7V9Nfe00b3c20esH8oIm/7GOd8MRj2uAup6I1azTmgXtD2Vd//UvNG/W+N1nutipzhr508keh7Vd/9YvQp98UjHvCvx/VGz5mrJuN7sYqdSY/772ReulnP9GHD96vjd6uWTIBJlGRPK+BL2oC64KOHdX16GPDOJZNmxYiUr5z+5805X/PBth/cO8/9cJ1P9aHD9yndcuXid+6pUtFmTd/d3PY/pA8+p/y/HN6+ec/dWvjDq1ZskSMc6VbdZR75ZfXa5oL3uKiDQ6zjaEfntf7f/+b970qWC5LpnwULDAsFNJbv79ZK2bOoPngxnrPny0wGPvQA2F8JS7Aws34J0Kg9kBgrx6pc9Ftzd8ZnzPapFQ6K1M5+QUqduY547VXgkbeuFNnLZ4wXrPeeEMN27ZVveYtlO8Mbe6IEVo2bbK4v3zadE194bmkmXCEYWxcs1YwvpDhfwiw1aJvPzX0+sucAX744AOCOea3bacP/nWPlk2fLsH8VfEz+RjmhQinBPlq3rOX5o6k36nO9Ba7oPL6LVuqzBn37LfeUlb9+j6+5mrUsZNUWqIVM6Zr3bKlXu5ByaQW/fbRsilTNOvN1yWf9/z33tM6Z7ANWrTU6H/eHZiw/AfzneACo37z5qHNiU89oRJ3Z62YOVOLxn6ogg4dtGjMB27tvBJgM8UZeMu++4j50WeZt+3NfPzfvPOyUhF5cslHk7Rk4kTNeOVl1WvWLMB69jtvKa9JE9Vv0Uof/edpZ9Kr3YoaHOY47cUXXMgu17t3/EnpdCqUmzPiXR9+mea6222hj6NFn35BuE566kmt8/WY8Y89rLKSYjXp1l3Lp07RWreQlrhgm/nGayHgWZkz85mvvqK1Dpu1S5YGC3CmC/cyhyODnujtFHsZ2itZv0FNu/fQglHvaZUL2qQM5WKKEIgQ2PMhsB2CYPNJlDkDK3UNV8646rdooVRWtkp84bOkeGNgFk3djdDI3QmNu3ZzJtbcmU+eYBilJeX3N2vN26Cd0N6mG2WBya137X3+B6MCY+5z6pfU11OrgftqwZjRgUFvKu4nq1yzX+8LrRtd017vrimY2rKpU12bne6Ms4X6uktr4Flnq93+w5TbqJEaueBqPWg/ZeblKZVOB6020y2Qfdwd0+uLw4NFQpslvp6Q17yFepzwxRBSOCM3T0UuuLxLZeTketutRJnSjcWevyYIgoycHLU/6BC3nE5V22EHap0vtq9291WLfv3Vy62p3sNPVtNevWli82QW2pryv+c0/rFHNP7xRwIjb+9tyKQGrVqr78mnKs+FQakLMMbZ4/gviFTki8/LXWgWO0Pu9+Uz1O/LX1H3zx0vd+yHvXFx021w5l/swnv1nFkqXLjQx94yWGwb3JrIcZhkZOcoq159Zbug3ODM3zIy1KBtOxn/UiYfjAo6dw2w7HL0MSpzgbBh1Sp/9uvV++RTfL6nqMcXT5bSKcnnoviLEIgQqDUQcKrd1lhNSn1cbL0z2vXOKDKdEU53jbWRu3O6HHW0WrgWb17O5URosMxPprvLob5rsF0OP1KtBgx0/vBxOxRKw2xatdSi8WNV6kyXvJINRZrmGu6CMWMC8yEvlU7LPLltIpgbeZVTmTOl3MaN3Ypoq4Zt2qjVwIHCymAM3qlS3k86Ozvsthbq+diSY2jTL8pcG09nZYnyljI5p5P/VTo7y1N2aIM5q6LuUtfaV86epXbu0up85FHKa9xE/FLpdOjHzJTlTJW2aDuVTnNboVFt4edzSGdmqu+pp2nQuV/XoPMu0D6nn6H6LmwpnZGbIwttlKmstEypdAbZYVyeI5kp9MNcvZ2senniZ5ZS/ZatVL91a4dJO7XZb4hyCxqp+3HHq/fwU8I9hOZMt4DynfH3P/Nratqnn1ctCy6xNf6szcyvpQxvkzGmM8vhQSZjUcX9tFuLCgANf7i9x6U4oAiBCIHqEEhVz6qcU6ZSd3fMf/99zXj9tcCgR/3tr0HTh0EVrVqtda5RLho/Tgs/GO0ujHVKpVNa666UZe5m2OCaKvdxdcwbOVIlrpFWbh0G0t7XB+a8+47G3PcvzfQ+Prj3Hi0c+4G7kzqpjWvtC90vP+Hfj2vCE49rnrtHWvUfIEuVDxv2RGrgTK6kuESlzsyzGjRwjXeBa7vrfKGzi1bNmaNJTz6hMf/6p9657VbRZ6kLHQRNsQudUN8ZZbGvd4z6x92a8fLLmvzMM0EDTzlDtcDTwh/xKyvndEH7X+fzXLtksWa/9YYK581VqTNz5ASJss6Z/WBq1KGju2je1pTnntWkp57SIl8gL7/ntyv9Z145+Y2C66qeu5wy3WLhNmPkSMrJL1DKzC2GRzX7zTc08fHHlJWTG+aaciEw3l0+Ex1Wb958k1tiG9TIrZ8it5SyvK1URqa71qaqxF06Hzg8Fo0bq7ymzZTh1pCZubX1gcY9+pAQ0HmNm6rUyxmT8emX+dFk4sffMhdGOfn5QlBOePxRTXXX1/t33+XTKvUilPBD/B8hECFQKyBQzlG3MtSUM9yC9u21csY0zX37TS34YLTa7n+Auh33+aDt9j3tS4IRrpw5Q22HDgtacXb9BmrqfudCZ5B9TjlVxevWus99stoOGaoG7t4oc2ZZubuCtu3V/4yzVOoChz5gOAPOPDuUbeRMrJe7Q1bNnhl8z/3OPEuNu3ZTbuPGgYFlOyMqcF9/frv27r75gpa7e2Te228p38fcrFdPF1jNgktnpdfHLdLvS6crMzdXLXwdYN2yJSrzsTTu2lW5jRoHt41zcvdzjxR5Hdy9Y2Zq1KWr0lnZModFs569lF2vnvi13GcftT/oYM339fqSNTwAABAASURBVIisevXVbtgBKnEXWb5bJLmNG1PEte0Wati2rRp7G90/f4KvHYzxeRap42GHK7fCglDFL5WRIdrPzCvX5CuywyG7YUMhTLjIql9fXY/7XJjHHBdAeW4xdHaLJCc/X4MvvFgb167TKrdUurlrKDM3T23dYmnavbvm+5rBEl936OTWWUMfU/fPH691y5ZpzltvqqBtO3U8+FA1791HzXr11hwXuIULF2i/r18oxpTn6xR57pJq5C4/xpBZv57PqXMQGF2OPFoZ2dla5GtELQcMUMqFp+IvQiBCoFZBIFXTaC2dVu9TTtP+V35Lw676tva//Ep1PvyIIATkv6bOGIdeerkGXXCx+rgPv6v7jjPcR97DfdfdXVg07txFgy++TPs5g6Kdnl/4YmCoXnXTf/M+YFQDzz5XQ70fjgUdO24q13bwkNDG4EsucwthcMhv0aevmnvfjVzT7nLEkW6FpNW8V28vd6kGf+NKdT36uODvlv9auMuKMQw89/wgXCyVUst9+mvfc85XQceO6n7McYGpFrTvIMoMufwq9fzCiYL5wpR7ONPNcSsjnZWlPu4Lr9e8hfhl5OQKP/3QK76pfb5yZkj1nFm2dYHXxBk/ZVr266/2LiBTzuRb77uf9qfsGV9VDxcKjVzIUSZJaW+/r8Mwr2nTJGvTMd8ZdadDDtt03bB1G/X3doZ4e339+eQ1bRbuNXBBu+95X9d+F10qYERmlguursd+LsBlv4suEYzdzMR897vgIg294kr1/OJw5RQUiDF087L7+3PY9/wL1MQFelOHc0uHYVM/7370seLXoEVLdfPzYFm5ckD/LfsPdCG0VvUdPriPKBdThECEQO2AQI2CgCmk0mmlMjOVcmZGkjMR8pNEXiqd9myT+ZF8S6UCc+Y8lfb6Xtfs4/vkV03UhYGk0unNb3m9pI/kBu2TvNNN/XAvlf64L65D2lL9ijyzzceUSlfU9/GHuv6HcdGPn4pzM+M0JMbA2DiSKMeRRAGOJM65V7mspT5uJ9z3P1Xb96zy/94n98ovyv9yDbw2tV+erVS6fA70V5Hlpyb6TqU3hy1tpDIyVbkNs83Lmpm4H1JS3zzPz6mf7wJ0ia+XzPI1hiZdu6oDllQqpfiLEIgQqD0QiBRbe57VHjdShEPrfQfpwG9+R4de88OwAI0FsscNNA6oLkMgzm0nQKCaICh2P/caXwBeu2K5YoowiDgQcSDiQB3BgeXLtX7VqrA2WlV2VBMEc8eN1Su3/VEv/enWmCIMIg5EHIg4UFdw4LZbNeLB+1W0bm1VOaBqgmBdYaEWzpqlxXPmxhRhsMfgQMTHSI8RBz4dDiyaPUfLFi5UaQmveG8uC6oJgsY9eqr32eepz9fOiSnCIOJAxIGIA3UFB84+V92+OFwZubmbSwG/qiYILJVSRlaWeJUwpgiHiAMRByIO1A0cgK/zBqiZqeqvmiCgAB91JcnMlHLhkCT5L7m3049lZaqpTbPNJ1BT2S3d86GH9jmStlRme/KoCzzMLLS3vXXMysdvVl6PNranbixTM17sKfBJ8ILnmiTyGB9Hs/LnzjXJrBwfOCdRpmo98kncM7NAi2abt8P9mGoHjnyWz6m0tDTwK23ht0VBkJRj0PPnz9e0adM0ffp0zZw5U2vWrAnImJTZ0jGdTod317d079PkLV26VKtXr96UPklbZhbGbWZau3atVqxYoR35mZmKioo0y9dRlhDSeTsbWbdunRa6f466zGMjX1LPnSuOuwpe2zm0WGwnQMDMtH79es2YMSPQCjQz159vcXFxoIVVq1Zp8eLFm3qCthYsWBDqkJmRkRHwmvpz5swhK9TjBOHAEVqEDlesWCFwhryYIgR2BgS2KgjMTCDxj3/8Y51xxhn66le/qjPPPFNXXXWVxo4duwlJGYRZuWZjZgGZX3311SAwuEcyK7/P+SdJZh/Xgxhuv/12/e9//9OLL76oZ599NjD2mtozK69vVs68H3nkEa1cuVLvvvuuHn300c2Iyay8bE3tcQ+p+uSTT+rrX/+6aI9xkW9Wvb5ZeR5EO2rUKP3qV78SxHz33Xdr9uzZ+v73vy+IfuTIkeFoVl6e9mKqXRAAD8aMGaOzzjor0AnHr33ta/rLX/6iDRs26IUXXtBll10Wnj9loa2f/vSnGj9+vDIzM/Xaa6+F+9AZ6cYbbxTCA9xZsWKFfvvb34r2uHfRRReF8tyrXVDabLTxYg+CwFYFQTLGhg0b6te//rUee+wxPfjggxo6dKj+9a9/Be3GzAKyzps3T8uXLw/CAY0GJo32Y2ZBMHB/2bJlWzRL0Iy4h7YMcZiVM0M0aJgm7aA1Mx4QnwQhcSSPOosWLRKppKQkjIF86tMv4+Ka9u+///6gyffv318nnHCCYOrUQUOnLywFs3KhUVhYGIQG9dDiacPMQt6kSZOCYDz99NODRo9lQF/0aVY+fiwXNELaoZ++ffvq8ssvp5kgwOrXr68f/ehHatCgQWASEyZMCMKTehQCLrTL/LiOqXZA4Pjjjxd4Br3AvLGisQ7AV57tf/7zn82sAHAZJeHPf/6zvvzlL+vhhx8W5+DO008/LXAqobfbbrstKB8oZne7MgHOUr92QCaOck+GwDYFAQjcpEkTtWjRQq1btw7I2qhRo8B40YDQXH75y1/qlltu0dSpU/XGG29oxIgRwir46KOPgibD/d/85jcaN25cYIIJQEBitGE05euvv14PPPBAYM6YzH/961/185//XNSFeBJmnNTlCBNHOP3iF78I5R5//PFQH+b/j3/8Q7QJMTJO+oFZQ2hYBCT6f+6554KmTtnf/e53QcBNnjxZf/jDH0L62c9+pqR/ymMNvfPOO2F+CBCIlfEzBsaMFjfTXWi33npr6B/ihWDJY3yM28wCM7j33nvF2GAEWChoh8AAFwNC4I9//GMQDmZGtZj2cAiYmRDwbdq0Udu2bdWrV69w5HmizOy7774BvyZOnBgUFjMTOPz222/ryCOP1IknnqiWLVuqR48e+va3v61+/foJgYCSc+WVV6p79+6BDhE25557rrKzs7eoXO3hYIrD2wMhsE1BgGaKRksCaXNyclRQUBAsgRkzZmjAgAEBgWGKMP+DDjpIIDxHmB/Ie/LJJwfNBrcOzFT+M7Pga4fxYWWA2JjQaOWvv/56YIDf/OY3g+CB8aJVebVN/xFQMHeEzvnnn69LLrkkuHw++OADkY8lATExDjSxffbZJwgyiI263Efbf+KJJ3TssceKvlj/wN0DM8cX+4UvfEEk2sOlBAwg7mHDhmn48OFq1qyZMNs/97nPiX4QClgLL730khCWP/jBD9SlS5cAK8bAukIyATT9KVOmqGnTpptgSNsIKwTHe++9F4i8wGHNM0jq1bbj3jRenhPKD8oGeH3HHXeEdTWYO/eaN2+uwYMHC/xIFBsEBIoLFiNloDFwA0GC5QouYjWCa+SDg9DQgQceGHCMOnsTjONcdw0EtikI6NbsY40URIVh5+bmql27doKR3nXXXYIBgtwwwPz8fDVu3FgdO3bU888/rzvvvDOsK1CP9pIEEzUzHXbYYRoyZIguvfRSZWVlBTcTeV27dtV+++0XNCusBLOPx0EbaO5o02jtP/zhD4MlwjVMG+HSrVs3oT0dfvjhgWgQYlg29AExIVywcugLLQwfLD57hBFEOGjQIA0cODDMBSKE6ND4YM4QNW4z+e9vf/tbWHOA2VMXOBx66KEBPpj7ELkXC1ogxySZWdDqEkIHZgiODz/8UMwDAYPQSsrH454PAay6f/7zn8I6xNpjXQ0LgZGbWcBz3IVYpDxbEngJwzcrx28zCy5HFCnuIyzAP9ogmVl48QBlhuuYIgQ+LQRS29MAiJgkNG4QsF69ekLjOfroo3XdddfpqKOO2tQUwgKGiFsEBs99fPJm5YieFIQAaBc3CMSB2wUzmjcoYMgIDoQFpjFlk3rJEWEEsz/ppJN02mmnBb9/z549wyIwY4QhUxeCYkxoU+TB0GkDgZW0T7/46TG3E0GRlENoJOfUI5GHRcQiH4t31157bdDuyacs/dIni8JYHtTZUjKzoPkzLmCB8AHGaIkIIdrYUr2Yt+dBwMz0xS9+UQ899JB4oQBf/4ABAzYpAOAFSgQWKG5BcAR869OnTyiPYgIeQAusMeCexC1LPaxNLFZwBHxmfWn06NEB1/c8SMQR1TYIbFMQwND//ve/64Ybbgh+eJAcLR3TlfT+++/rvvvuCxosi74gNkfe7EETgrmzeIYLB+YGMwZIIDeaMH5Q3qzAx37PPfcETYg8/Kb43m+++ebw1kXnzp2DP5X6JBjkAQccoFatWgkXC+sT9EufCAM0M8Z80003CTcL48Jauc/HCgFSH+0b18wtt9wi+n/qqaeEJo8g4D7jJNEfxyQxdhLCkLKsM6AFotVByPT/zDPPhLUH/Py4mSiftJMc6QONLy8vLyzG4xJi/CwQduzYUbRPvaTfeNyzIcCz4tlWTeSTknyEPS4i3KnMCDzmebOeBc6Di6y18TIC+IBliLuUfHCacggP6tEmbcQUIfBpILBVQQDiwqRwbRx88MHCvYHvn9cm0WjQxi+44IKwyIUb5lvf+lawCtB4vvKVr4QFr7PPPju4ZkB6FruGDx8emHnlAX/pS18KmjyWw3nnnRfcMGhRnCMQYMycw7A///nPB1cN99GWEQIXXnhhMLcpe+655wZ3DG4eXt8j75hjjgnMHTfOxRdfHFxN5OPTh4kzB8rQHusEEBdCh8U7iAxhxX3mxbixVnAltW/fXmhruLOYHxYPrwMiXGgDCwWfP9YK/eHmApa4f7CimA9946ri+ogjjghuIoQC7ipcUzwD+oxpz4cAuAIzB294HbTyiLmH8sRz5pliNYL33/nOdwSj55mzzsWroTx36O3qq68W55RnfYuy0ALn0Bd0SD3uV+4rnkcI7AgEtioIaAyEPdz96zAwEJdFX5gbiA4CwsRgdPjhWUBFIMAoYer7779/cJVgKrPgyn2Yr9nH7iHagEFDPLTdoUMHuhVtIHhgpjB/GD43EESdOnUKaw8cITAYMu0zDtxEZhbeTOrdu3dwF+GygpnTF20yVu4hLKjPItxxxx2nU045JQg7hB/zoix1IDYIEu1c/uM+2hj1uA9hnnrqqTrkkEMErBgX8AEWwA0hwTULhizwMRbq4JZi3lxjWQEnhCvfSuCuYoyV/cLedfy/B0MAXABvEPrgSOWhcg8FgXvkc41igYKBMgEe4vqEbsB58BkBYVbuNjSz8KIDuAsdgkfgCu3Q3m5Osbs6CIEaBQHzxSeJr56E2wOkJZ8EIpJHGdwcCePiSEruU4ZrEvUqJ8pQnzKcJ/dojzzuJfnUp/8kUZZzypE4J49EfcZcuT55lOOYlKVtypCf5HGkDO1wn3OOXJMqX3NOP4yNdqhLGa7J5z7X5JNHO+RxpDxH7tE/+dRBc4RRUC+m2gNkTS4tAAAQAElEQVQBniPPcEsjJp+U3OO5J88/yeM+z79qPveT8twHj8iLKUJgZ0Fgm4JgZ3UU26kZAhA61gcuNqwps48tp5prxrsRAhECEQKfDgLVBAEMKaay8CbPZwUHNMvPqu+k33j8bHEgwj/Cf1fgwNbERTVBgH+TN2xiyg6LtxEOEQ4RByIO1BUcYL1yS8KgmiBg8YpFLRZLY+obFpAjHCIcIg5EHKjtOABf56UFXsbZXBhI1QQBBcwsfARjFo9mEQZmEQZmEQZmEQZmtR8G8PiqaYuCoGqheB0hECEQIRAhUHchEAVB3X22cWYRApUhEM8jBLYKgRoFAaEOCI5FwDdWsGmFd50J9ka8E+4TCoI4PdyLKUJgb4UAb3oRBoIYQf/+979F4pxIo0TPJbwK3wcQL2hHYUQfhCshQi30SOKccBTEJ6rcLgHvoNHKefE8QmBrEKhREBC47YorrgiRFBMEBvGI9AmiE4fozTffDLH1t9ZBzI8Q2BsgAFOG6RNXi2i47G9BXC7iUKEs8bU4zJlrGPqOwARBQvA5GD/9ETOLOFmEf6/6kRmBC2fMmLEj3cQ6eyEEahQEIBsIRiRQBADwIUQywbK4R9gFQjjwuTuWAveI3U/wNcpSD2QknzwsB8IrU4ZQ0ZShHmXeeuutsLENeSSQnuiKBLVLhBD5RCpFw2KTGwiKxNgQWpSH2Pj6krIkNvagbfYCYMzk1eUU5/bZQIDXrlGaCDJIuBPCixA99Lvf/a4IYULAQ/bjIMHAwUWi3YKb4CjX4DZ4Cm7DyKGByrOhDPRCHvT0f//3fyEwITGr+BIda4H9EGiX2EbsBUJZrBFoBlqqbDkQOr5y/5SNae+EQI2CAJAQE4cAaYRSBhHRRojpQ2wU8tj8BSZMhNLDDjssBKG75pprRCRQwvASQwdEBQkJrEXgLAKssZEM8ftBemL1EHeHYFvs1AUBQERJWYK5UZYIo0RipD732PUM9xTRSwlMR2gG7rHTGPmY6uQhrIjfggBiTjFFCOwKCCS0whfivHfOq9i8qsdudQgA9u3AYmDHO6LqEp8KfGXrSYQD+eAqOEtcLnbZqzpOMwu7AxJziLa///3vh5helIPuiEdEBFMilLJXCG5cgjFCXySseWiTfULoizwEFcKDNmLaOyGwTUEAshEAi1DPaN5oLAREQxM3M2ENoNGDWDBxtH8YOpYE2gsB1gjJTF0QEIsArR0NCsRHKyGAG/uywvARAoSuxpx+4YUXQghpwvYSiwfkJngdmg+uKeqAwATHI2InWhRj4IiAoT0I45VXXhERGwnji1Wydz7qOOvPCgLgOpFJYcJYDUThRUtnG1ZwGiUHnOdjHwIrsscFuIsbqLJ1a2Zhpz82vUERQ9mhnWReKGpXXXVV2CeE+/QL7rOBFDSK9g8NQh+EXCfiLu4rIpkmFnrSVjzWBgjsvDFuUxCYmfgIAUSC+WINsDsXSMcwzEyYqUQIJTw00UCJo04Z7hO5E7MYNxHRFmkLoth///01ceLEsHUfggULgC3+MI/RjihDPuXRfojSyRiwMGibSI2MhTzCSSdRSvnogyimCCwWy9gH4Sc/+YnYH4G2K5vGjC+mCIHdAQEzE8yZhOKCQoLFiivHzAQ9oXTBnMFfoteC1yhTlcdnZiFsO8KAqLVYw6w/UAYaITow1gjXKGMweCwPwp9DUyhm0Be78WHpY1WztoF7ijox7Z0Q2KYgAEHRKGCgmLWYrGgaCbi4z1oBbiLeLsKfjybDzlwgfFIW5g3CoZHAkNksBqEBEmMe//73vxd+Vcxh2gAxKYu2xG5P1EHYYHlARAgREsSAtQDDB/HpFwFASGAsjRNPPDFsEIMmhsnNWJOxx2OEwO6EAFY0Gj47kbHAi/bOHgS4lJJxQDOcc0xoh2sStAaTJww6bifcoWzAlCxAU4dEWRL1wXfe/EOgQGtYyggM6ASXFAoYytOf/vSn+NIHQNtLU42CAMTDVQOiwoR5S4hFKBguifswYbR28onHj9Zx9913Bw2HuiAgsGXjFsqygIXWwm5c+O1h+j/4wQ/E+gKaO4IGvyXlcAnR9iOPPBL2GGBzG6wSmD/+TRg7Gg7tI2TYCwDLACuE8Xzve98TiE7bbEoDYeDKonxMEQK7CgLgPClpH1ohoVDhznz55ZfFOgL0Mnz4cPGSA/SAoEjqcQ79lJWVJc2Eo5mFI3/YQAlBwnoD7lLqkLhHf9AnblrcseyHgcVO+xyhZdYOUMJQ8NgPBGuFujHtfRCoURCgVfMaHGYlC7p33HGH0CZg1jBxNO5rr702LFaxUxfbNWKqot2j7WN+8gYFYMUMffDBB8WiMoKCI3m4iO69997g16T+z3/+cyF4YOJcs8B24403CkQmVgbrDXfeeafwbf7617+m6bCRDRo/+RAa52hOuJHwhbLZC8KEhbVQIf6JENiFEIC5g3t0AcO/6KKLlCg24Cj32DIV3AZfWUhmVzvoig2SUFjY7hQayMnJoZmQcBWx5kU5MwthYFByoA/qgPeJYoTiw06CWA/QGi9uQHe8bsp6GmtmrKfddtttQhBAv6GT+GevhECNggDtGQSFqaLpo6GbmdDI0b5Bchg5yAqSosnjisF6AJr4+NmikXMSu5GByCRcReSBwCArBMCbQLRFPn2zpoAlgcAhj8Q4EC74PVlcIw8tiPLHHXdcsCySNrjHGBBi+Fzpi7yYIgR2JQTAebR1+sDvjwKDMgVeguPcR7FJ8Bi6wvLlpQzWvcwsbNmKVVsZZ3H7JOXMjOaDZYFggC4QAihX3MAqZq2Bc9qFvqAPxkEeid3zyKcf2iYvpr0TAjUKgtoAEoQUZi3IbFZOHLVh3FXHGK8jBCIEIgQ+KwjUekGAxoQZjbn7WQEx9hshECEQIVCbIVDrBUFtBn4ce4RAhMDeCIE9b87VBAEfqfAqKO8ax7QofMUZ4RDhEHEg4kBtxwH4Oq/ks6ZaVRRVEwR8L8DHXzHNUYRBhEHEgYgDdQkHEGbbJQiQFLx/HFNZ+BYiwiHCYQdxIOJPWcSdPRF34PFVUzWLoGqBeB0hECEQIRAhULchUKMg4N1iUlUQkGe25Vc1k3tmFj54qVp3Z1yblbdtZuGLY1X68RaRmVXKURhHMi6Oir8IgV0EAfCLbweq4uEnzd9Fw4vNRghsEQJbFQT4kfgSkfDRIHFSm1gpxBIiqFuSlxwpx5e/hIsmHhCfsZttzpSTsjt6NLOwgEu0RiKi8uUwYzWzECuFLzf5XN/s4375/J5yxCYiOqrZx/d2dBxJvXiMEKgMASKHEioFPCROFjTBfSJ//uhHPxJfEhOk0cxEuAeig/J1/gMPPCBCsJhF3AReMe1eCGxVEKDVEOuEIHAwfzMTWg7RPtmTgPsgeZLMLGjnxPwn5DSfrxNdMfn6t3I5VfzMLNRJ7qniZ2ab8lXlx5jYFY0YRQS5I6AWfjja4OtimD7ESB5VERKMl0USvrDkc35Wzs0iwQGfmHYOBMA/lAwEAHF+CCpHqBWYPULghhtuELgI/hFqxcxEDCDCtkBfhJxA8aLMzhlRbCVCYPshsFVBYGYiVgkRC4nmaWbBxUKUTz5dJx4Qcc2xAGC0SVx0BAShbwmvizXBijuMF8Jg/wGiH0IcJM6pD+NGgKARkc8eA88++6wQJDB8MwszMjOxOxrtEbvFzISggZAYFxFKCeRFdEfefqIS2hdj4JN7Pq8n/AVlE0FBmZgiBD4tBMxM4D2x/dk5jNDp4C55KCsEeGPDmO985zvhbTRe00ah4at4hARxs9ing02eECqfdjyx/q6AQN1tc6uCAM2EaInEAQJBYZwJUhPOAeZPgDmQHO2foG5oQoAKAoBZ4yKCCT/++OMiVC6aEUGuEBIwY7b1I++ee+4RgeFwJ9EXQbK4jzCgbQSEmQUrgTYZG3GLGBOCg7GwiQ0CgfGSh3sosWAoj/DiSIwkXEoQouIvQmAnQQCcI+YPkT0Joogl3aNHj6CoQA98/Q59EDcLvCQPpYYYQeAlQRqJ3QWN7aQhxWYiBLYbAlsVBDBZMxOhntHQ0azR6mHAMFUYNcHcCCFNdEWsBrQZMxMBtQg1TcA4dlyCCAjCBcKjrZPQ9rnPrk1sZUmwLPrEdIYg2HgGgcO+BaxHmFkQBLiD6tevHwgMgkqsCoLd4fohTjtHzHSsE7QuCJLNceif+wiWKAgUfzsZAmblm88QfJGtWLGI2SqSbsBV8BttnyO4iJuTfO6TzMrXDTiPKUJgd0Jgq4KAQYCsMHDcLwgDNHQiGJpZYMpESiTqKO4WwlOzOCb/gewk7sFw0eph6ERhRANC80eDIjJoTk6OiBOEq8mrClfUf//7X7FVH+Yy6w1Ju2blhALxmJXv6mRmYZGNtQvqM1bcRlyzXoD1gUBhPNynLoRI4jqmCIEqENjhS/AUmkFRwq2K8oFlCh2A8+AgVgE4SB4KDfRhZmH9AJqAHnZ4ALFihMAOQqBGQUCbICuMFV++mYWtJdn1CER+4oknhJsFSwFXEKGlIQTqgey4hWDILOKeffbZIvwuJjHIXlBQINxJvMnDmxOUhVAQPGy2wSIam8qcdtppgrBg3CTagqjoh8TuSmyNSRtYJLSBNYLWj8sJwUPYbOoyLtxHWAokrmOKENgZEACfoIff/e53Ytcw1sMQAuAz+PfYY48J65UNlCiL4gRdsUkN1isLyNAUrqMEV3fGuGIbEQLbA4FtCgIzE353XgVlf4BECLATGIh+0003CUGAOQxy4+JBeFCHBWC2nIQQeCMCywC3DaGjcQvBlCGct99+O1gYMHbaRViwlsCrdmYmmD+TQWOCsaN5UQYXEoSDX5Z2cVdRDiGD9YFZzpghPPIRTowZgcU8yIspQmBnQADcTHCNtS8EAVuv4paENsA33iJCGFAOXGRvAmiBbSJRiM4880xBO1EQ7IwnEtv4JBDYpiAAKdFqrr76aoHQIDwd4Pf/1re+Jd6Q+O53vyve1iEf5MflgzsG986wYcN01llniXeov/3tb4tdwtjMhkVf7lGXdQje6IFhw9DZXYm1B/ocPnx42PSbcSAosA7QnGDoCBt2WoJ42A2NTXQYn5mFsf7yl79Uz549g9nN2GiDfsnDhUReTBECOwMC4BZKEOtl0AT4y+5htI2yAk6zHgbNsIEMuEz+hRdeGGiI3cigG9qhTkwRArsTAtsUBAwG7QVGDQOujKhYAKwPcI8y3COPcjD1RPNGc2fbS3ym3Cfh3vnFL34hXrND+2e3JPJpA8besmXLsAUm7TCGJFEGAYDvH22KxWvuUSdZZ6AN3E8QJq4i7nPEZOccdxLHmCIEdjYEwHVwF5owK3/tmT6wasnHWgUXySNhKZCf4C55MUUI7G4IbJcg2NmDglFjBbAOgFmMKc0iNFrStvoyMw0cOFBYHNtTPmmPPhEMrDkgXLhO7sVjhECEQF2GQJzbtiDwmQgCBmVmYfGY10Q7deoU1gjIIEgCKQAAEABJREFU31aCgaPtY1abfaxxbU89Xh1F86KNbZWP9yMEIgQiBPYWCFQTBDBJNO3dkXiPmrd7OH6S/lgHIH2SOpSlDonzmErD2kmEQ4RDxIG9Awfg7UmqKuCqCQJ87l26dBELxDF1jnDoXGtgEJ9VfFYRB7aBA7yMw3ruNgUBbhdcKDE1UYRBhEHEgYgDdQkHcI1XflkhEQjVLILkRjxGCEQIRAhECOwdEKh1gmDveCxxlhECEQIRArsPAlEQ7D5Yx54iBCIEIgT2SAjUKAhYYSYOCnF8SIR7JrYQb/nUNJvkTaCaymzpHiEnSFu6F/MiBPZ0CBBCGhqBVgi7wpGPGAk0t6WxE4WXGF1burejeYS2Jm4XNDh69GgRiqVyW9ynX2i7cv6eeR5HtbsgUKMggOETGoJ08803i7hChH0gjsrWkJs6EAExVT4psj355JMi7tDumnzsJ0JgZ0KAjZquvPJK3XjjjYFWfvvb3wqc5iv6LfXDF/Xs57GlezuahyBitzMUKrbAZNOnpC1ok3hfBHvkNeokPx4jBGoUBDByvsKF+d9666265ZZbdNJJJ4lIiTNnzgzQYycwrISFCxeG99JB+hdffFHvvPOOOOcdZfYQIABcZe0EpCRMNO1wTjheojeyUQ3n1COeEPXQmhgLwoe2CE3N1pPkhUHEPxECewAEwE/iXSEAoBcSO5YRdgINnThXaOMEcGS40Ad1OAfHwXXCsHNN4t7s2bNFPWiCPGiIMtABbcHwyU8StES7vP3Hx5rsx8E196nHxk98mb+lVwgpE9PeCYEaBQEg4VUjQjMQQZRdlIgayhFEJLIoG8IQLOvggw8OW0uCtHfddZcILoeG9PTTTwviIDQ00RVh4CA4oSWIEMoWkpw///zzIlTvb37zm6BFEXmUTW2IK/SFL3xBY8eOFXWJTXTIIYeIOlGr4QnV+lSnJpDEDmKPDd7ZJuYQmzp94xvfUM+ePUXAuZ/97GeqzMDZ64OIpOA6IdWhIYDyq1/9SkTWhb7Ae4QAdALNEbhx0KBBm3b2ozzJ7OOv7WkTQQKtcm/GjBkici8RUc0+Lse9mPZuCGxTEFQFDxYCAbSwBEBUhAQx1YksykYyBJojoiJaEQIDxn755ZcLzYSyMHA2qcFkZQMaEoHkiER68skn65prrhGI//jjjwdmj5YEQrP9n5kFAqIfopYylqrji9cRAp8lBO6///4QiRdGTUh1lCF89ihUDzzwgK666qrg/kTDNzOhFLHlKzhOKGoYPvfY3YyNoLC+aQNcJ2Q7VjDaPi4eYnUR6n3JkiVbnDIh2xFEkyZNEvWgUwQRASC3WCFm7rUQ+MSCANMTDQfEREvHtQNjxq3DlpLLly8XHy0QC4jFM9xGIDSRRtF0uEazh9mj0UAw5513Xog0SgTRgoICsaDFfsns8kTYaY4IHgiEaKcEnEPz2mufWpz4HgsBLOPrr79e0ARra8TRIkouDB86wB2KJQtjZhLgNNYB4dv5kh9aIDoua2zQCKGssSTIh6HTDnsjYz0QsZeIvtAkbVVN0BLlULxwp+KuZQ8EogNXLRuv924IbJcgQJtJwMSewpiahKFA22AfAmKvs0iGNsNXeCAryI3lAENnPwLKnH/++TriiCPC5hv4/yECGDyLy/hIIRDqYSHQB5oUbWEVmJlAYARQ5fEk44rHCIE9AQK4UBEG0MWBBx4oQrawKxmMn7W2U045RZWVGHCa9TDCqoP/aP/gO24lhAYMnPUFaASmT3lowMxCoEbOtZUf91CaUNCwLLAEcMdupXjM3oshkNrW3Nn+8Ytf/KLwK6KZ/PjHPxY+ezT1yy67TLyhgO+fDTl41RRrAESnHMwdbR4hgDDA7QNjx+9pZiGUNGsEmMZoNWzdhzYF4uJWQmigFZGHZsQCF8IDwtnWuOP9CIHdDQGYfVXcNDOhDOEChRauuOIKQScs4MLgYdaEYEdIQF9s6YovH2GChYzLFBrArcQ9+qAeR/pKzpO5Ql+V81ibox3W7FDUECRJ2XiMEEggUKMgAEnZUYkdl/Dzf+c739FTTz0lzFgzC75QFoMpw1rAX//617CtJFoPuzGxJgDzv/322wUBPPTQQ+ItCjQbdg/j9TYEBmY02grm7w033BCEDvd46wKf6j/+8Y8gfBAyXGN1JBOIxwiBPQUCMF3WuXAFVR4TjJ6XH8Bdtmz929/+FnbdY1c93uBB0UruQytYE+ygh4IEXcHE+SaBsihRlIc2cb+ihHFM+sNdxJt93CePdrDE//nPf2ro0KFk7YYUu6htEKhREKCBo/3zxgMJJt61a9fN5ogP8pJLLhHMH82Dm/jxv/KVrwitnoWtz33uc8J6YD0gcevAzCEEtJyWLVtSTQW+PkAeBMUrd8OHD9ell14aEBiEJo8NbbA4QoX4J0JgD4IAbwrxyia4WnlYZiYsWmjgqKOOEjiMMsTbdNAIZdH8uQ+NJEycMrxFBN2x1kA5LHEEArQJvVEPVyr3SJTjbbuEzsiDnrAGoEWuY4oQqAqBGgVB1cLxOkIgQiBCIEKg7kEgCoK690x314xiPxECEQJ1BALVBAELUCzcxrRREQYRBhEHIg7UJRzgZYItya5qgoAvD3ltjTcXYpqhGTNmxBRhEHEg4kCdwAFeR+Y15arCoJog4NUzPgTjozFSTKsUYRBhEHEg4kBtxwH4OmFKePV4m4KAAmYxDglw2JMTb6Ykb5fsyeOMY4sQiBDY8yFQzSLY84ccR4jPki9NiUcToREhsPMgEFvaWyGwTUHA+8pon0lCC+UdZTMT9/QJf2bR2kjg90lARx2SmYUIkizqE/obc9WsHKY8G5KZhfADir/dDgGeEc+AZFb+XBgE+dAQNGP2cT73YooQ+KwhUKMgYFGB0NBsSEM0URIREF9//XURB+i55577xONn1Zp1iE9csY5UgIETdnjFihUyq84QzEwwDEIBkMwslCPgGKEJEkZCwD8C/BHPCdAQeoNwH3yhOsMX9kaNGiXyzIzbMe0GCJiZiCDKhjN8Dc++AwgAMwth1NnPgy/xyd8Nw4ldRAhsNwS2KgjMTAgCYqQQ6pZAWCSCYxEdlL0IuJf0ZGaBYZlZkhWOZhbyuYDBsbsZG9eYfZxvZtwO5cwsHEOG/zEz/6uQZ1Z+rio/Mwv3q2SHS7Pye2a22XW4qPTHzKq1YVaeZ2abSppVPzezUNfMNitn9vE1N8xMxFEiCiQMgkUbM9usLkH4COxHmOF///vfQmBQjp2nYCDUIyDfM888EzYs4Wtrs/J2uY8mSsx63vyiDIKDviuleLoLIGBm4Xlcd911Icw0Qd4Io0IQxdGjR4t4W2wKQ3gJnu0uGEJsMkJghyGwVUFAi2YWoicS94c4Qnfeeaf+/Oc/69RTTw1aK4ydcjAgNFT2HIDRwbjMTKxQEwIXQuC1VFISXRG3BiGp0XQ5EoQLQcNWlePHjw+7m5mVExfhq0eMGCGYG23TZ5IIbEcfJFbFyUdIwSxhgmzMwStTJPLRxvGtw2Api4ZOGbYMRItmjGYmGDLlYNrJnHDDMAbqoW0zTgid+8yf0MFYPJRhHlzjzzczqgRXGrAgTjxhAbjHeChLOG8KMWZCbRDZlQRjZ16Mi6BkwAnLgDAFwI1EPe4RpI+5EKSMzXt4HlhuCA/KxLTrIMBze+GFF3TkkUeKGFnEDCJEBHjH3huEirj77ruDQADPoZldN5rYcoTAJ4NAjYKApnDj4ApCk0fDJKY6yM09EsyMfVlBco5oPDBIGClBs9itjDzqsw8BjB/mB5MkoBYmNMwRIvr9738v9lNlww3Km5kQPuSzMQ17GuD2gLGZWXitk7JsBkJAO/qDYUJotIsAgSBhsi+//LKoT1nGypgYO2OBcNHgyPv73/8uXC5obZwnRxgs1hBRIBEeCBrGxaYglENQIoyYC8Ly8ccfF2PAfYZwYMwQP7BJ4svQdlKWeXKPOEqEDoaRE8YYgYEAQ/OHsSD4EKLHH3+8iNYKk0E4IhwICsiciN1EwD8C+SHgeE4x7ToImJmgExQAlB9cQOADsYSIqQVOEv8HC5vw0gh3cMGsXEHYdSOLLe/1ENhOAGxTEKCBwvxhgKSHH344uDfMLLg0CFONtk6wLCIvEnjrrbfeEox+2rRpInIpIXbZhAYGyKYaRFdkPwM03a9+9auC6dHGaaedpmuvvVbkJdo580DTZZMPojoiSGCqJJggTB6meMIJJwhmjRuLwF9mJvolCBeMFWZM9FKC5xEtFUZOXbRmNgIhWipjhcliwiMgIFg28mDMaOkQMokxwXxpE6HAORFZCbSHWwZGPHz48BCdFVggABkvAgZLgrboB3gSERK4EagP2NI+7SaJ9inLpiW0AUxph0QAQBg9zwAhQdAygpoRXMzMhJWAEKEtxhzTroMAzwmLkWfBMyX0NBE/wTHuYTGAJzxDjjznXTea2HKEwCeDwDYFARtrsMcqWi+aNBosjBZkpis0IZgm/tCLL744uI5gVjBatB8IgiPuChY/STB0CAPXBxt5oMXCvIiqSERFNF00YJgd17hS2AKTyIuUpV8zE+4dGD/bZLIxDpYLhAdTJOIiLhs0MRZUGS8Mn/4YEwkmieVCBFXqIKgQUGhrF1xwQdDyEEAskDNHiNisXAAm54wFAYMAxFVGmzAAmDKCBS2feZiVr7mYWXijBwGLZXXjjTeK6K1YTvSBq0mVfsCXTXxatmwZBC+Chd3gGB8wRzDSB/MhwiUCl7GYmYAzQoC5V2oynu4CCIDP4CjKAPsOQAs8M3CQ5wDempXjAGVRMnbBMGKTEQI7BIHUtmqhueBnhrmTMH9ZLEbLgcHAZBEM7F+MhsveAoSuRhOnLEyKNynQlGFyZhZcLzBC2qB/hA0Mj0VSmB4+cxg07g36pxx9cc6ROiTisOMrxyV0zz336Hvf+57Q3nENoSkT0hotO9HIyYNxsk7BXGD69IFVgADBrYM1gICiDnsv4y6CuVIHoQQsaIO2EERmFvaDZYwIB9pk7wZcWYzr3HPPFcKH+whBxg9zBm4Iq5///OfCXQX8zjnnnLALG3NLEm4F6jJXYIPVw5heeeUV8S0BY2QtA3hRjkQf1MdNQT+Mi+uYdg0EgDfPFkWFZwJuceR5IZRx0bGPBziGIDcz4fKj3s4ZUWwlQuDTQWCbggAEx9cNw0IDJeHHB4lh4DBSNsPAfYTPnG35YDzdunUTPu4//OEP+tOf/hReN2Wo5MNUIRa0cDMLW1eiRcGQ2Y0MgYC7CCZGH4yBupyTR98IBZg+mjDWCpYKC6fcx82EBQKThAnju6cObWDVUB7hhaXCTlAINgQYr/1hlcCgMfFh5Ph66Ys5ItwgbNYlYMi0jXaHgEDDo49jjjkmLGr/8Y9/FK9y4gpCI+ReQUFBmCvWEswB7ZG1EWDEkXaAHeMkmZmwTqiH1cE8YDa4fGAklMfthVBlrcLMqBYSY0ZgAQfGGDLjn64Gz58AABAASURBVF0GAYQ7OIx7CCsP3AE/gT8LyOABtMPbX4cddlg1gb/LBhYbjhDYDghsVRDAuGBu+NTZIQnXS5LYaAYEZ0cxmAxaObsvoQnjEoFpwpDxm7OA+c1vflP4wWFy3OOaI+Upxzi5/u53vyv6wNUD8ZDPegFaNoyNzTVYi0i0XhZW0aLpm3HSHowavz67OOGqYQcnNHrqwOTZ6Ib2GY+ZCcbK7mmMiXzGjMA57rjjxFoCbVKHLTUZK/OjPzYLYbwIQnaEwi1AHwgR6tAmiXsIAu7h/mLPWCwlrvHnJ33QJoITuDNvEue0y3yYC24u5sM59UkIM6wOLAbKUw8443ZiDYb+knzuxbRrIACMcQWxZSvPErcga14oMTwjcAEcI6GE7JpRxFYjBHYMAlsVBDRnZmIRk0VJmHGSQHh8+DApysGYYMCUIw9GBGGQD6MlwQTJgzDQ4tFyaQdBQhvUoS5tkI8QojyMNqnLGxhowuRThyPjoH0WU7lnZqIO+TBKmDqWBxobCaKkf8ZBG2Ym7lOfNQLyaZexc82cYbKMj3zGTX9o6MCGcriSOCbtMU7q0U+Szz36R4NnzYC3f2iTuTJn5k6ZqgkhwrwkiXbpq3IZ4IR1gZBK8hGauCYQ2MyNcSf34nHXQgB8A5d49jwbYE8CX8AJnneC87t2JLH1CIHth0CNgmD7m9nzS2K1kGDGn+VoESpYAgg3s49dOTtzTAhAtjDk9UWEws5sO7YVIRAhUPcgsEVBgAZT1xJvHpFgjJ/l3MzKrSzcWjDsXTEWNE7mika6q/rYFeOObZaFFw8iHCIcysp2PgxqEl/VBAFMBG21riVcNKTPel64enA/cdxVY6Ft5spxV/UR281RhEGEQW3CAfgBvGdLAqGaIMAHzQIj79zH1FcRBhEGEQciDtQFHICvs06Fp6CqMKgmCKoWiNcRAhECuwoCsd0IgT0DAlEQ7BnPIY4iQiBCIELgM4NAFASfGehjxxECEQIRAnsGBKIg2PXPIfYQIRAhECGwR0OgRkHAO/fsSsbXknxRy1ewxPQhJENNsyI+DmGVKU+8lZrK1rZ7vH5KbB9iKPGa346MnxhFSeylHakf69QOCBC7ii+MScSwqjpqwlFAT3yJTniWbdFV1fpVrwlsx6ZPfM1Pm1vCMcKbEI6dr+WhaYIdEqeqalvxeu+CQI2CAKYHMpmZ+FKSr1/ZNeuWW24JewFsDVTEuGHvAspX/uJ1a+VrUz7ESsA3xmy2Yx+EARP2Nqi8wxvtxVR3IEDocKL2ohTBaNmhjMi0lWfI19/sg8GbeuxbQawrmHnlMp/knM2RiLnFa4J8AQ+dEnerchvs0UHEXiIBQNN8rc4r45XLxPNPAYFaWrVGQcCcCGxGTCHiDRHfn7DMROAkaByCgqiKhFG++eabg3AA8e+77z4RcRQEoz4Ij3VAIuAc7WJtELyOwHAEZ4NYIB40FKJmoi2xYQyhGAgmR7+UhWDolzZIbABD0DaQnsBeaFhYMASRow3apH3aIj8hDPqgDmNngxyilNIu8XmwZuiLYHtVCTOZF+En2AyGOVCP8QMDopYSYZK9EtAEb7rpphBwjw1y0MAINQ2hEpCMYHyEm2AeMdUtCBASnTAnBGskESaEgHOVZwlTJughe2GAm0S0BS8rl/kk5zB/2oFWfvKTn4jXBYnEW7kNghMSNwu8JxEpGMWkcpl4vvdBYJuCAJAkLhAzExE4ibUDA2OHLZgb4aXZUQyEp7xZuaaMIIBZEmiLaJ0EWzvrrLMEchKymiBvMG80IQgHpg9jpz0YK+GZMWVh4rhizCxsdINgoR8SY4Bh82EH5YjwSAyfxx57TAgI3DBENGX3tBkzZoRAchwph/YE4dE/7fAVLsyZfNqmfnLONYkQ2RAcXwYjoDDFEQRECUXgMAe2t4QY0QCZ5xlnnCGsCDbb+elPfyqEJVFYCb0NfGg3proFASw+6AQBgDLE80Z5qjxLwoCA9zfccINQSoheSkyipAxMG5yiLWgQPANfOU/KVD5Cm4cffnj40A36QengvfGkDMoX9AQtYA0MGDBA7OxHflImHvdOCGyXINgSaGB8MGA0CsIpk2DQuE6IuElCq3755Zd14oknis1a0J4J04xLBM0crZ2Q0ORDFPSTfPlmZptC9cKgYb6E773mmmvUo0cPioaE1nXVVVeJjUCI4YOWA/JTB8FDISKWEhYaZg+h4btFG0PoENsfAUagOuoQDpqxE+kUoQWjx6qgHRLEiSlvZmGDGYSOKn6M3cwEYQEX2kbY8TEK/XMNMSP80MJgFFhXFdXjodZAoPpAwR2YOjjFeoCZibykJOdm5QpSkoeSQghxlASsVzMTShD3YfaEOycMOnSEoEAxIRQ590lYq7h5UHqgPfCO+igc0AH0ktAV5RkDdESEXaxWlBVor6qAomxMexcEUtsz3crMDkbIAjCMEyRGW8EVBDIT2RNkA4lBStqG6eGHhEnSDhE70WooR9RM8riPhk152oRZgtDUhdET8hmm+fTTT4s47+x5kLSPJQByUwciRBtPNDAsEtpnrLRPW5zTLoyYc8pQH3cNbaCt0wYbyyCsmBPzYWwkxk2bnJuZ0LzQ7JkTwtHMgoBA45L/6BM3Ev0xVpJnh//0TZ/hIv6p1RAAL2DGuBjBH3CbPS3ADXCVsOCsmVWeJNo+blf28kCpom7ClMELlCaYPAwbOgPX2IvCrFyg0DbuJOqhsIDXKFlYsYTAJjQ7oUaSPsFjIqNioeIeOu6440Q0VHA3KROPeycEtikIYMj4NkEwTEpcGzBatHJCKsPoQCjOyU/CHoN0gBQ/JYiOiwT/Pdsssi8AjBJtB+aOacziMpo2WhIEwloBhARzZcEaYTF8+HDB0CEKCI/26YfENQQIsdEOWwRCGMk9jiTKIYz4zBqXEYvfaOoIOPL22WefsEcBeyCztwDzo0/6IjFHCJJziIzNdJgX2hvrC+Qn/XBOfyTySMk52hzlIUTKxVS7IQDuwHh5+wdGi4uGlyZwNWIN4tpkPQCcxhoFD6AV6Arch5mjYBDGGkigJEBTWLDs2c0Odqyxgd/cJ4Gb7MJHn9yjD/AQxSMnJ0ds8IT7EtzmTTfwD6sFvKdfyqJ4IbRoL6a9FwI1CgKzcjcHG7DAhM8555ywPwFbQqJFsxjKLl1oNSDV6aefHmL7wyBBUjMTSMxm3mzMwYLzOd4Gm86w5y7IesoppwhTGn8lCInZyiurCAAYMYwXYQIx4aqhXTZiwcLgsXHNOX0yHkxdXp+DYEByGC6MHCKjPOcQFwt0mNAQEhYO7ZhZ2O6SNYQzzjhDEChzws9LXVKfPn3Eegdto7XRD3NHOCEcIWC0f8ZkZsG9lbTNGJgPRxaVEXpoZrQbU+2HAEwcJQQc69ixo8B31q1I1157rXBZosHjl0ebh67AI9yQKFkwaOgJSJiZzIzTcKRt8ChkVPwxK9/dDyWJ+zB7GD1vK7Hx0qmnnireSuIFDXZNQ1iRR59s3ISCBh3yBlFFk/Gwl0IgVdO8YWZo8DBlXCb4ynkHGYZNPZgafksYGswcy4B8GP+Pf/zjoL1DFLw6B7OdOXOmYOIgJIIEBop2zaIwFgJ10arw7YO4mMXks48Ai6r4UdlqEiKjLAn/Pvc5R+NC28K8hthw77CTGkSI5g0DRqCwcIePFOuGt3sgCqwEMxP3cDExLkx9BBRtJwktDUsDzQ5BwK5hLCAjgHiDCtOb9QoEJ3VYAEQIIhyYC/1DuFgShx12mBAklIup7kHg85//vLAKeNMM2jAzkYcihRKE2xEcBa/BIdayPg0UWEtgjQ5LE3rliDBCGcNqhe7AQaxt3EHgIDsDfpo+Y926AYEaBQFTRMNF2yaBSORVTmYm8mGySb6ZBT+5Kn5m5WVoqyIrHMzK86vWpZxZ+T0zC2XJYwxm5dch0/9Q1+zjPMoxHrOP61PGi4b/nFMGQkGoQSi8xcRuXuRTiDJJG1xXTggMrALemkILY6MZyptt3h951ONI4tzMRB8IGYj0/PPPV/zVbQjwvEnJLM1si7SR4Ig+xY9+oJEkgcO0a2YB71TxMzNRhnsVWfGwl0Ngm4KgLsIH7fyrX/2qcEGxPnHdddeJdQuzjwXK1uaNJsfbSbyqh3WAWwyC21r5LeXTBm8mIVS2dD/mRQhECEQI7E4I7JWCAADDjFmDYHE4cRuRvz2JNYMk4V8127YAqdwuLjfcQ5Xz4nmEQIRAhMBnBYFqgoDFVdwmMa0OH35FONQlOMS5RHzeu3GA9V7eHqsqcKoJAnzXvAHDa2cxTVGEQYRBxIGIA3UBB1gL5bV6XnLZpiBAWpB4zzmm0vB1aIRDhEPEgYgDdQEH4O1VhQDX1SwCMmt5isOPEIgQiBCIEPgEENimIDCz8Lobr5qRzD7Zwqh24s/Mwsc1ZuXHHW3a7NPV39F+q9Yzs6pZ8bqWQwAaqZzMPn7GlfM5Nyu/Z2bVaMys/J7iL0JgN0CgRkGAGcH78nxIRuIjKr5KNNs6kppZYNa7Yuy8f8/HXEn6pH1AfNQhbAYf1JgZl59Jwk/Hxz+Ym5/JAGKnOx0CvGjBF+PQSpJ4xjxrviRO8pIjoR+IQwRNJXkcyae82WeHnzsdOLuqwdjuToHAVgWBmQkEJlYKcUyIokkivj4LDmbVkdTMBIPlK2CQe6eMsKIRmDify/MFJjGF+CrSrPoYKopvdjArnwtfETM+voJmXwPa3KzgbrqgX8IB82UzX5VyvZu6jt3sIgjwDHnJgg8UoZMk8Z0K4cgJCZ3kJUe+XyGcCaFOqtbjC/SIG7voYcVmq0Fgq4KAkolFwBewBL0CcdFgEiSlDO/EEwOF6J1mJiwIYpigHZnZplg7yX3qVE5mJu7xXj/ElNzjIy0+/CIl+VgkEAevwHE0s2BSU4b6Zh8LBuon46I+r00RuRRrgrAQfPKPoDOzEL+dstSR/5IvNBkXbXPt2eE/bfFVZjJv7pEoR34o5H9oizYZF3U8K/ynDGXpGyYAPLmRtMeR65hqFwTMTOBY165dBZ2w3wUJJYr4QdAFISWIJ0Q+iZDqRNXFaqAeNIbgQFCg6BBlt3ZBIY62tkIgta2Bw+T44IoYJQTNuvLKK8XHVGg5mLDsvAXiEnOITVnQtNG8iW4I8hPXhCBwfMWLFmz2MbOGQRLADSuDgHFo+2YWQjsT44iYLOy0RAz2rblQqENcI8qxz4BZeUx3NsphQxwiMxICmGBcMF4ijRJniOBfzI2IjRAfYyQCJJYM8WGIzUI4X4gVC4T+zUzETCIWEfsosA8CbRGCG42OcNwwdoQUsY6AC7GQ2EIQOMMo6J/YS9Shf/IRcAgpYMvuaFyTH1MCgdpzJLYW9MJX4yTiciXCHcWAPD5C5Eg5lA0ULuqRTx4fOh544IEihlftmXkcaW2GwDYFAUgKEyTB5EBm4uuglROeGuZO4DQ0GDZ1AZmJ8w+iE8+fAHIgN8yeAm7NAAAQAElEQVQc5p4wP4CGVkwQOXz/lCfIGxo77XJOwDiiORLQDYFhtrkQwRSHYdM+gopzAs5hahOfnailrAdgltMOxIYGhoAiOB3fTMB4EUj0hfAi4igCjvDYjBWhx7gTomTezJMxE+4XQUh0R7Q+AoghROibuRNZFIsAoYDvmJjzuKUIT8HY8QMjeGgfAUPICtonfDDjBkYx1S4IgOcoPWy6REK5wB0JLvHs2RaVfBL4AL6Df9TDIiDYIsoDOAs+1K7Zx9HWVghsUxBUnRiCAQYOYsO8YapYBzBKTFyYL+GYCd2AUICxsQtXcj9pz8yCxkMdwkvjfiIkLvcpTzwfdj07++yzBZNnXYJ7STIzYQHwkQRMlQiPaPdo6IyHSI7EE2IPV867d+8uBNQhhxwiwlAzfoQBbhriBVGWENkwdYiT8ZNHWGwECIzZrFwQIfiIFUQkVQQMY+Wc+SJEEI5ENKUuYa6JO09fhAkmrDV5zBk3EQJl3LhxwlqhbwQJFghCKplrPNYeCIAPaPQEJkS5QFnATQjdEHGXvCQRqRY85B71yOccXAFvuUYBqz2zjyOtrRDYpiAwsxBdFKaFGQvjxtWCpsv2kzD+Sy+9VETvRLMBkc0sbNeIxgOyg9TDhw8P/vzKgDIrZ6y0m5OTI4gBwkDQYE5DQPRrVl6ucl3OYc4QGv5VtOwTTzxR9JfUpy1CZZMYF3UYI8ckQWjMhX7oE6uHMjB/6jMGxpfUpx7XlOUcCwlhYmYhoiN5Sf/UpZyZifpYAPRFHm3QN4n6zAFrBSsCYcV92oqpdkEAhYM9NthjAEWAZ8kzBydwrbJzGPkkFAieMzjA86ce7kRoinU28NBsy7hfu6ASR7unQ2CbggBmiz+bnbxw0bB3KsyXhS+YJ2YvbhBcOfjAYX4IC7R1GCl5iauGcxhiAhQ0c4Kv4dIh4afn9TkIhtj+7EeAsEFDok/qJok2Bg0aJBgxAgSrAfcRrhzKsiZAfdYuaBtiY7wvv/xyiCHENXsPMD/WAngjiUVw9h+AcLlPHyT65JgkrklcJ8fkHMJmLLTHZiOsJTAnGAQb8rBGwA5srAmwoE4oa4QXwgFmgADCImGstBlT7YIAoQiglUcffVTQC3iMhQgt4CZN8jlyD+vVzMIX7Cgh4A9WMFYu61yV8at2QSKOtjZBoEZBgEZCeGYQGWaPj/Oggw4S2g7uDhAWlw9uDZgcGi3mL2/l4NrA/QFyQxyHHnqocBnB/AAQCA5x0AZMEMRne0gEDBvbsMkMAgShQn/k9+3bVzB5GC3XmM5oWAgKfKwHHHCAYLiMGU2M+mhiLLwhcNC48NUjLBACHHELMSbmdvzxxwfLhjUQzHvGiFDAzEfgcE0dxsGYGT9zhXkzJ8bN4iDbFLLhBy4emDraH/2zaxTCC3cW/l82KQFezBuhiMDF1UY7WAm0GVPtgACKA3iD+xEXH5vKg5OcQyO4DVlHAie5R0JZAr/BaXAZ/KIdXK5sbsRLA6wh1Q4IfMJRxuJ7FAS2KghAShgdfnre3rn66quFxv6FL3xBMC8QFjcGeezAdc455whGikYDw4O5gdy8ucPbMDB8mDbCJYEAbcDU2VmJbftg/mbl2+9Rn7zLL79cME/qHXPMMcJ3j+lM39SH+dM+JjXuKTRpxsDOYNTHLQWhmZmoT1/UYd0AIQFTv+yyy0RZ/P64kdgBDcFF+zB+hByMGpiwPsA1jBr4AA8sEjMT86MuWj5tATfaZrxm5fNCKAAT6jEW+qPNM888U8CYPISUWXQJJHhSG47gBooQr4vyHJMEbeC2xOoDH5J8juAtCgc4eNhhhwWrgLmCl0OGDBG4Ai6TF1OEwK6EwFYFQdJpwljRgEnkg/QcSdwnwahxC5EHgySPchxJle9TpnKiXtJ2km9m4fsC6iZ5lKPtJCX5lNlSffKowzgoyxjIq1qfMuSbWfDlU44y1DGzsLuTWTljNiu/lv/MLKyfmJXfox2zj9uAiMnzouE/4zCzsJZAH4ybG+TTX9Xy3Iup9kDAzMJ3M+BS5cSzJvF8K+dzTj6J5191ptw3K8etqvfidYTAzoRAamc2FtuKENgcAvEqQiBCoDZAYIuCAA01prJgHUQ4RDhEHIg4UNdwoKpwqiYIWPjEb8/CVkxtFWEQYRBxIOJAXcAB+Dovs2zJDVlNEPB2DF/qfoKkWLZlhEHLCINIBxEH9nQc4O1F1qS2aRFULRCvIwQiBCIEIgTqNgSqWQR1dbp860AMI17Nq6tzjPOKENjlEIgd1EkI7BWCgNAOfMTFV5wIhDr5JOOkIgQiBCIEdhAC2xQEBI0jvDRfvJJgqvTFF8OEl+B8e1NShxX4muqgtVcNR1FT+S3dI3QEXwxzj/7MTCyE86UveST64KMxzmOKENgZEADH+aoe3Npae+AgNEXZymWgJ+pWzgc/CUzI18mcVy5PH1sqTxh08sH7pDxt0gb0DH2Rz33agK6rts39mPYeCNQoCGD6hGUgZANf4pII90Asfz6RZw+BTwIq6vGl77Y+mydkBGF6QdJP0n5SFkK47bbbNHPmzJAFkhMOmH0L+PozZPof9hUgHgz3/TL+3wSBeLIjECDyLPtSENKEL9OJdZUwXdrj/Pnnnw9f4A8bNkxEoSX8CkwaPKQOX73zxTFMG2WGuFR8yU7IEr5aJtwLdEmsKsKoQJN8sUx56IUdBWmHECZsggMtUOeSSy4RfTI2QmFDg4RT5wt+vmJmLAm9MNaY9i4I1CgI0BgIgUCMdJAaxklsHYJqEUqBOD0gH2Gj0b5BLlwvICrX7A1AWGW0EMBKeyA950mCCROUjfjrRDUln1grCBy+yiUGEeGluQ/RQEy0QYA58tFmqJMk+iDcM69IJUyfcmhhhH7gXlKWkBNsbFM1xHVyPx4jBD4JBNiQCWFAEEOYNntdVMYtNH4EAcwXxk/4EgLPEf+KAIU/+tGPlCgwhCaHuYP3P/zhD4UiQ6h1FDDiFRHZl82YEAjQHZsfQUfEBEPhIdgha2LgOwEh+Yr9/vvvF7TM3iHQJfmEfqFt6IlNpT7JfGPZugOBGgUB0wSBiJNC3CDiohxxxBFCUwGRiOqJicsuYCApCPW73/0u7DBGlE2CzhHTn406IBDaM9v8k3mEBfGKiLVDvCICr6HBIHg4EnyLe2eccYbQaiAOojKyfwF57PY1s0Lzp30EE8G8CD6HIEEoQRwEciMWEZvEQDiUJUAYcYLYw4DrmCIEPg0EYNDEy0LzRpEhCB3KS9KmmYWwKcSWgqa4j1KDQEAIEJOKzZG4RokhsCOxqnglEVpDkSFCLZY1Wj/KFvSCYIE2eS0Q9yfvvBPfiFfBUZywHK699loRn4u4XbRD3a9//evCGkAYQStE3k3GGo97DwSYaYo/NSU0aXbcwgogtPJ1110nkAzNGy0CpCYSKAz5F7/4hWD+CAXqoBGx0xdl0bxB1Kp9oZXArO+77z6B9GhCCBqsAwgB4YL2RCA24rczHq6vuuoqofFAAFxTh7bxp7IoDJM3M+Er5RoTmn0TsFjQsihLIrIoeYlwIC+mCIEdgQBuGJivmYX4VDBlrABV/LCucfGgsYOfKDsEaoTxE3od6xbrGnyEtog1BG2RzzU0AS6D4wiGW2+9VShF11xzjVDIoEU+GDr66KNFe/SHAoQg4R50xi56KD8IHYIxQk8oUlgTuGQrhhoPexkEahQEZiY0bLT7n/3sZ8Jvj4+RiKQJnEBWwi6DoMcdd5xYT2DbRVw6+EvR3Nl6kbd2EAhJveQIQhKKFxMVQUN0UbQk7ptZICgYPSGsYf5YBJjBWB5YBGzziNaVEBwakPxHgC8/iBjwmN1EF2XcuInYqyDpAyJECCTX1IkpQmBHIID1nOAf9cEp8jgnwaxxDWHlgrdEyOWI6xTmjraOS+ecc84R+2jQFvQHY8etQ2RacBl8pS5CgPYIZ85aAgIEfEdAUB7Gz/4b0B3uJ9oE/4noC93SJ1YAriz2GaFv6IuxxrR3QaBGQQACoZXjh0T7hoGizaBRJGAC2dGw+XwZJMMnSohmNBPcSGg8hx12mNDokzqVj7SFPxW3D1oSwgM3kpkJQsAXimCBCCAqEtoN5jcmMWYvwgTEpl2zctcTdbnG1EYLI6Qv20eyKIbfFLcT9zGRMYvNyuuRF1OEwI5AABzHosUyQBFCu0ejh45IMHWUHtw6LABzpByKDNY0mjkaOmWwJtiPAKsamoKpw8yhLdxKrK2h/GAJcw/8pz8UINa+cIOyjgdtYk385Cc/EZYF9EV96uHS/fDDD0V9FLmcnByZRTrYkWdf2+tsUxCAwDBKEAxESdw7MFqEAD5+3D+4XTBDeSMBsxRkQ1vhHloKzJu2QLrKQAMB77jjDuG7R5D07NkzvOYJoiN4fvnLX+qll14KbiMYOZYFVgl+UrQj1gMQIIyNdjlCLBAjbVOON5UQJBdccIFIzAF/LuPB3EaIUY/6MUUI7CgEsIzBz1NPPVXnnnuuUEDw6WPp4iplHw+YM64ccPnmm28WLkvWA8BXlBT28sDvD55CcwgBrGq2YaUNGDwJ2sMi5q0+mDn7X9AX1gXrclyj8PAWEu4gLASUOfZHYC8MFoyha95Qom2sAty6WMg7Ov9Yr/ZCoEZBgPYNE2VNoOoU8c2jxcNMQdrDDz9caPZoOjDmn/70p8IVg0XA622YvQgI6tBu0h6EgJZCOe5BHCz0skBMed6MgInjdoIAuAcyUxaTmT6HDx8e9gWgTRbCcCPh7zQzQUS4m7hHQiAxXjQ1riEiFsJ5y4jrmCIEdhQC4CuKy+mnny6YPa9Xs2YAgwbfYLK4KNlECa2d1zvPOeecsFc3L1TAlBEiaOq8/ok1ztobNIjgwOXDYjQ4jGsU3EYY8KYRClTHjh2D+xaGDrMnn8VgrGcsDjZOSuiI8bAmB+3RNpYHdISStKPzj/VqLwRqFAQwR5gtiFd1imy9h+sHpgtyYpqyixfIRGAjEswcfyh+SNpi0YoFXzSRpD3qgagwdrQhiIW6aP0IG4iAe1gcIC6CAGYPouPrZOvMyoKFfnjFFTMZ8xrNiD6S/iiL8KEMVgF94VpK7tfuYxz9ZwkBmCjuIV6rxm0J3pGHEgSjZmzgLkpPVdylLPjPmzzQFPUoD37CwKEBrGyzctcNdMKbSfSFADKz4NbhjSEEAXRDPviOC4r60BDpG9/4hugD+kAwcA9lKOmTfmPauyBQoyCoraBAWCBIWAyraQ6Y0RAfrqSaysV7EQIRAhECdRkCdVIQYBWwiMyHaTU9PIQFVkdNZeK9CIEIgQiBPRECO3NMdVIQACCEwbZMXVxUZkbxmCIEIgQiBPZaCFQTBLy9wEIrbwPFNE0RBhEGEQciDtQVHOBNSt74rCrxqgkC3i/mw5eYloWvNSMcIhw+UxxYFuEf4b9zcIDvU4i0wKv52xQEFDCz8AaCWTyaRRiYRRiYRRiYRRiY1Q0YwOcrp2oWw/MKSQAAEABJREFUQeWb8TxCIEIgQiBCoO5DYLsEgdnmUvCzAouZfeKuzT55nU/cyQ5UMKs8rh1oIFbZYyFgZptZ1FUHamZVs+J1hMBnCoFtCgJCQvDhFcGxiNtDSIbPYsR8wcx3ARy3t3/eGiLWCrGKtrfO7iiHr47QGGaRIewOeO+uPswshGjn2RKtF3phca4yzpqZeBkDv7dZfP6769nEfmqGQI2CAEZKPB/ikLzzzjti442bbrpJMFfumZUjspkpuTYrP+da/jOzEEHU7OOyZhbKJ2U4klTpxzWvgJpZKEsgur/+9a9ioYN7JO5zNCtvW/7j2g/hv5kJIUCwLfLNLLTFufxnZpuNjXwz26yMKn7cS/qryApaX5Jn9vEYzCy0Sx1V+nHNK6vEj2GTH+pWuh1PazkECGBIXCECJbIHBjGy/vjHP4qgcsnU2FjmlltuCbvngQ9JfjzuPRDYE2e6VUEAkqKBw/yJafKrX/1KN9xwQwiSRTArNBqiH5qZIACuiZxIGFu+2CW6p1n5PbQiIi/SJveoRyRFGDSMkTqUr6w5EXmRaIy8xWRmImTEe++9J+Km0war30RVpB2uAS71uU5ej0JoEPiLT/N5LZbwvYyTtmHCzI82sHqoS9vMAeHBmBgv7ZpZeIOI8dCvmQUhQD3qU4++krLMD2ZPX0kb9Md8gQXjIGgY5WOqGxDg+RK3CuuZUCjEHCKWEF+5o0QR6oGw0Sgz4GuCo3Vj9nEWtR0CWxUEMEYCZhE7BS0GcxemR6RCoiSy0QxBrWDkhN795z//qTFjxoggcT//+c/DbmJsnkEALWKZEJUU5sdmHATAIigXMU/Ya4A6BJZ79tlng8ZPJFKCyRGcCwGERoV2xbu87MmK2U197tMe0RVpm13TsFhg5mYmiA/tjKiLECGaGNv+EfiL+O2E1CaAHWOH8RPgi7HRLmNj5zIYPNo7AcG++c1v6uqrrxaCkPKMm3Lks7EOTJ8IqgQbow3y2XgHJEGgEueFeTEmxkh+THUDAggC8J84VsT4AR+hDeIBEdQNPCJ4I7hH7C3oq27MPM6iLkCgRkFgZmGDbbbUYz9TzFyiFMKYieiJ1oumTphpQukSsweNmQiKBJuDgaKRw3DRgrgHgRAwi635+vbtG0JMwyAJrEVoXCwDjmy2AdOEmSN0iG6KUIJxk8fuSkRjJGgWmj1+dzbjZlwE9koIzaxce6dfLAG2xSRCahKeGobNugdtUIegXWzAQ9AwBAQaPEIQqwgXGXVh7sAA7Q/ByHzpE4TALwy8CCpGcDEEEAIKFxvhgYkyScA7GAPlY6o7EEA5INS0mUkV0yKwG1FEsQDAd/ALPDOzihLxECHw2UNgq4IA7RYGCXMl0icaO8wNpGbhmA1lCK8LU4R5434BwYmWCDMmJC6xfogsyjn1YH5mFjapoS7MHNOZ7SLRltCqcOEgYJ566indfvvtYjcy1gdg/kRohNA4Ryiw+Q2JsNcffPCBsEzob0tgpW/6IeoixEi0Ra4ZG4KJ+9QjUiNjIVYR7izcRERVRYujH8L6UpaIrER+RGhh1dA3wgSrCYuBzXyYA+4h2mDMtM3eB4wRwQG86DOm2g8BniX4gbIBfjAjMxM0xA59uBHJJ3EvpgiBPQkCWxUEDBLGiyaLWwPGBeMmH+0eQUGIalw2MDssAu6ZWfCfQxgwdo6kyvfMTOSRqpZhMw4YNPsZ3H333SGuO2GuMbPRqqhjVt4HREV5mDuaOMIJxk4+/VVNjJn65NMv5ZJrMwtuKVw75GHm0x/CDEGEZYDFw45S+Phx7SCEsEqweLAQeBuEUNvEmEcQ4IrC0gFu1EGo0SdtYx2ZGUOJqQ5AAIuTTWCwNHGlstERz5zFY3AK/AWv6sBU4xTqIAS2KghgWLg42FwGFwl7ChPnnF3DcJuA6FgBIDjaMVqzmQnhAZxgupyblTM7NGLyKU+qek558tHW0ZrZIxl3DO4oBA9jMTOxZgATpixtkCgPY0VgJP2QD+FxDdMnUYc8+uKaMma2aVMb7rOrGrtLsYZATHc28CCWPP2y4cgDDzwQFswx+R955BFhKeFKYnxYEriO2JWNfNxOWAoIE4Qm6yW0DQyBH/3HVDcgAF6hDGH1YcmyFwf4gzUIvST4xmx59uAg5zFFCOwJEEjVNAiEARvJ4M9HCJA4HzhwoNCWYci4avB5m5nQfNkxCSRHM6Y85/Xr1xe+dI4wa3Zfoj4aFIyTc7R6tt1DeOBLZ93gy1/+smCmbD6D2c35scceK1wrtAHxURfNq1OnTsJ9w5iTOXGP9rEwaAN3D3m4hQ477LBgAeCyYucz3EO0x0I4Gj2LytRh/KwBsFjMeFjsg9gRPlgCSXnGhtUELCiTMAIEAovuCRyZG2sfCFbGkow1Hms/BMAV8Jwd+aADNpnhHNwEt5ghiglKAUpUZVzlXkwRAp8VBGoUBMmgeAsCRsaOZGi3ZiYWQBEKMHdi+oPUBQUFYrcvMxOMlbUCiAPmDqPniOuG3c0oDzNNCIK1BRg0BAPjxBfPQjBrAWYWXEn0gxCAsJJx8CYRLhiEEJZJZeZKH4wdbZ37JPIQKmhv9IV2Rh+4mHgNljkgMBgvZYEBZdhlivGQz5y4RzvAhfKck2dmYv0DYucNEurSj5mJ8QND1kWSudJ+THUHAmj+WJBsRYnCgLuS55/MENwB31BAKucn9+Nxz4VAXR7ZdgkCEBYGS+KcBCNGQ0bjxU1CHglmCMAqn3Od5HPkHnkcud7aOf0l9ynDeZKoS4L5o12fdNJJYW2CcpUT5SmXJO5VPueaMggCFsNxBeHvJY97SeK66nhohzwS5zWV5V7lNjgnL6a6BwGeLThBqowXyUy5v6X85H48RgjsbghslyCoOiiQGI0G9wpHELtqmd1xzThYm0Dzxi2zo33SDusDtIMlw/WOthXrRQhECEQI1DYIbFEQwAi3ldB2klfitlV2V95HCO2MceysdnblXGPbZcFFuEvhUBb7iPCt+zhQVVBVEwT453H7xNRKEQYRBhEHIg7UJRxgLZN1rG0KAvz9+MlZ0I2ptSIMIgwiDkQcqAs4AF/nxRleWNimIKCAmYWFV7O6eozzMoswMIswMIswMNu7YACPr5qquYaqFojXEQIRAhECEQJ1GwJRENTt5xtnFyGw10MgAmDbEKhREBBXn7AIbESTNEVsdaKK8q59kvdJjsQlIsw08Xs+Sb3tLcsbDwSC45sAooYm9fj6mHvJddUjb0HxpXTV/OSasBDMO7ne2UfiFCWhund227G93QMBYmOde+65IjTKBRdcIMKQE1eKfSiuu+46cY9AhUTw5U03aIigjoRp//vf/x6+dK88UkKrgHMEMqycH88jBHY2BGoUBGwKA3ITOhkGTudsvvHyyy8LJOb6kyaii4L8xAb6pHW3pzwChlgvRDAlSBx1+PqYvQMQBlxvKT366KMiZtCW7pGHUCH4Hue7IvH6KsJoV7Qd29w9EOD5JQx+xowZIgQ5OEdAQiLU8uU69ynHiFAuCK5ICHWCF4J/CV2BD+xxwQY34DTlY4oQ2FUQqFEQ0CkhFwicBdKCwHx4xVe47Mr14osvUkQwde4jONhFjA1dCFTHHgbEaGfzGJCac1asSURoJKAc0UshFhpCK/7b3/4mdnHCCkGDJ8Q1G9awmQv7HlCOBKGgbVH2rrvuEhEeKY81wE5ixAkiFAWElTBxiJExEgWUOnfccUcIc43GRjhtxsIWg4QSfvjhh/WHP/whEDPhpZk3ib6TxBjpD62N8lhQwIS2mQvlEKBEcGWuHNHyJk6cKMbEfeqwcQ7jJF69mYk5E7USayyBK3MjvDWbAQFb5kj9mCogsAccsAQIVshz41liERCahGdFaBQUqt/97nfCWiDcChFz2biGOuAiOEjYaqbyyiuviH04CFfCdUwRArsSAjUKApgPX+wSYwcmCUM0K19hh9HB8BgcjJOonDA1NGt2CQOpr3NzmMBszzzzjGCEMDeEAHVhnjBydjMjXjthngnQBQHgfoJg0JQmT54stCKYJdoU/ZGI6on7By0dC4NopQgnGC/12DAHwcUcYL4IISwDtDN2IcOqQWARL4lxIMyYB2Y4wohd0IinhHXBmOizaoJQmSMCj53TCDaHQGCeMGz6xqKCyOkDRsA1cwJetDdz5kyxbwFCBQbCONgAB/gAJ8JZM040TDbVQYAALzRIhCFtxLRnQYDniRZP4EEzE7jN8yXgIIHowBdwk3ziEhGIjsCIfMNDPZ47OENAQ+JxgUd71gzjaOoaBGoUBEwWJCSoGhoMzAut2Kw8CBzITBlSco7GTVRRGDmB42BWnBMPKNHaiQ9EBE4YH1oUTBkBwkYwRB0lz8zC7mUIDoK04drhSF8kiG3o0KFh8xqEDJYLlgPB3ogmij8W1xBERkRQxnTVVVcJiwXiYkwweSKW4u7iPjuKETIYwcc5hAkBw4TNjG43S8yVd3MRLERaRTghtL7//e8LwYMrAEHFNXPFwiDMNe8kY4UgWBEKCFs+9ACGMAEsFjRF5kCUVawO9mLGimIPBPrC1wxsFH97FAR47igtMHHClWDpmZkOPPBAERyRIIUweZ4996AnJmBm4nniOmW9AKFBYELum1XHPcVfhMBOhMA2BQF94QoirO79998f9iUGYckHkWH0MDASQoOv1ghBDRPl4zRMWxgy59yjHvGBiMpIu0QjNTOhtbPLGAwOQQDDRDuiLwQHRGVWThD0i9BAiyK6J4wUN1BSnj4gII4ks3LBhVDAaoF50x51GR+WBuNnLtzHIsAVxRwgRrPyfmmrcqI8c0UI0RaCirlRD8FJW+TD+JkrAoj7wIN+sTpg7rgPGBttMw42v7n66qsFw0fzxzVHlNbEOrjiiivChj1YMNSJac+BABYneI4SxKg4J7z6D37wA4FLKBjgAO5QjjB+6AbBgOLA2hbWNLSGxYuChKKDgKG9mCIEdgUEtikIYHYgKj7P008/XWgrMCuQGDcKzAxTFxcHA6Q8KTmnbHKe5IPs1MHlwtoB2jAhmtHO0fzRqs8555ygRcH0aYMx0A4JJk84aOrSDltnooVRn/v0U7k8xIigwRog/DQuHTaPQdjgt2czHJgzaxBo47iWiKyKlQDBQqBV20z6IZ9z+mOsyTljRkAhELCkmCsWCPlo9axhIHBwDxCum3a4B1wRDFe59YIriX0QCF0NwwDG5JOwUoAj/cW0Z0CAZ8hzAZ8Q/IwKJYO3hMBpLF/oBUUBxYh9PcBdcBiBj3LCs0fpQskhXDW0geICztNeTBECuwICNQoCGCgaL8yMcxAUtwsIC5LiQuGVRxgUGi5l2FeAPQoYLFo/2jLn5HEPhMYKgOH+3//9n9CEETCYzLTN4i8mNBo/MT4gKNrBMqCdJFEW1xBuGTQm3Fdc0z79MOakLBo5xIk7iP0SEBicU7d///46/PDDBQGyrsD6BO4YmDTECdNmjBAu40ja5NYGPyMAABAASURBVAjTTvKYH59vk8+YIWAzC68M4tdnoxuOl112mYjYimBFUEDwWDxYDsyXPRPY3IR1j2uvvTYsKmNJUAehdOONN4rFd2CGMKS/mPYMCCDIYfxYp+Aho+K5srERrj2sAlyFXKN44IYEh66//vrwsgMCHnwgtPs111wTXj/FgsBNCH7R3t6U4lx3HwRqFAQgKb5tGDdDAhlZBGU3Lu6B2CwEg8gIBPJ4UwLkpTymLe4NzsljsRPGymtylEcrhxnTPsKF96n5xgBGxyIzjBUGjSsERkw7SYLZw1zRqDCfKcP4qMP4EFRJWcZFPyxuIxAYI5rZ448/LsZEHQQEFsI3vvENMQ7avffee8XCLa4qGC/1kjY5YjWgsXOOYOJVQM5pi0VohBcCCqHCmgZCjrdHyGes99xzj4AR1wgnXD/ME1hhefH6IUIRGCBwWGtgXMCIOdIG/cW0Z0AAhQEmz94YiSAwM8HcUSqgFZ4rigdKEwoKL0tAB+AmFkLlmfDMEQ4sKFfOj+cRAjsbAjUKAjMTmguMKukYDYfENVo3Wi3XJDMT2jAEwX3OKcM5eZShLY60C4OmDPdJEA8CgTaTfAiGc7PqfnrapCx1OKcNs/Ix0A/XSaJPyplZsEI4p25Sj/LkUY487sFouaZ/5sExaY9j5TzqUJZ8xpyc0y6uAObK0ezjeXBNG0kdrpNzBAJ1gJNZeR36YFyMk3PKxrRnQYDnWfXZmJnAB54nzxWcYNRm5bjKM+XZm5U/Z+6RzMrvg09cxxQhsKsg8P/snQWAV1X2x7/n95thZuhuEAlJFYMwULBbbN01sNbO1V1X17+5uuq6du8aazd2C1goSgjS3d05MPG/n/vjDT8miQGZmfuDO++9G+fed965p+5758a2FeAAN2AgYCBgIGCgbGAgCIKy8ZzCKAMGAgYCBrYZBoIg2GaoDYADBnYwDIThBAwUgYECgoD3mXktNKTpCjgIOAg0EGigPNEA0Rd4zTm/PCggCIjAyQdMxLsJaY4CDgIOAg0EGigvNMA3UrzmXKIgoAJvNYQU85/8lz08hHGHZxZoINBAQRows7ydJ+HzyamARZBcGM4DBgIGAgYCBso/BkoUBIRNKMynFKEGM4PEtZlxENdFtUkOxeArl+IfxkrfhYGkX0JFcCysPDmPsRcFJ7ne9jzfmvFwz1vTfnveZ3npC1qEjgq7H8r4Sjz/M+GrZAIZ8rzMEvGxqMN1Mhzgkp+cF84rJgZK666LFQR84MVXj4SDwNTK3ynlBE0jERSLgFsQLZ/RE/I5f32uCQzHF5YEZON6axMTihAXTAzi+RBHKP9YzUxE++TLXfo1Swiswvo2MzF29hXID6ew+ts6z8z8fg/EqYEBmBU99sLGYmYiXAeBzFj/Mdu89oXBDHlFY8DMxAsX0BrRdvPTELRK7CHCl/OVMWHTzUzsUUH4dmISMT9QWghJzl4FBGOMegQ2X9KzT0X40CzCSjhuLQaKFQQQMQTKSrOZCcYP8fH1JEcYE7FxCBGBsCB4Fl/ftmrVSlGsHdpQn68tgQdTIs4/2o/ZBqYEPFJU1yxRZmb+S2DygcUNA4dz+oLBE34agUBYCcaCMEqGRX2YIAKLCWZm/l4imGaJvoBNW8ZOJFHO6YdEXeCQuBfyqE/inHLyzRKwonrkU841dTlSj3zGSB6JOuRRZpaAQT44Jlge46aMNlFdzqljZuK8sPbcA1+tErRuRxFuKsc/FBJClbAJEkqP2YZnCY0S2gStn3DpvI0C0yfGFSHK+eqYECTEpIJWmSMoVdSBDsxMBEYkUCLhys02wC7HKA23th0wUKwgoH8YFwlGgpaDhUCIZDZHoRwNZ+jQoeIaAiV8LkTKJIB4ibxJ/B5i5hAB1Mz8IqySfhA8cX8gfqKPohkRpIt+gfPAAw+IfQrY9AWY7NRFP+xpcM899/iNXZhgxPZnopiZ0IAJ8EZsJMZuZp5Zmpm4Ju7PRRddJDQuJqZZYlKZmQjvTCho8onrw31xz2wuw/0SB4k4RDBZGDRjJ2YQY+ftApgykUGJDUQi6ig7n8HAmfxofcSQQdOjLq4CGAcxjojDxFtbWv8DZ8AiOit4Ij4RIbKJNcQ9giPGQFwi7peYSozRLHE/gKEP4hWBI/BJG/JDKl0MmJnYkwLGTuwonmvUg5kJ3PMMCSJHmGqeN4yfCLTUI14WcaYOPPBAQS9mJmJzrV692luFCBlogTzqhxQwUFoYKFEQ0JGZCaaIZgIzQTOFGUHAMDYIn/g3xEwh2iguIkxeTFjaENSNqJ7s3AVTAmaUzMyvKaAFEZgL2AgSGDVMn2BchPYlABfn7NYFs4fxYY0Qp59+KYd5U8YkYjtHonZifRDJE6ZNn2hlhK+mPywIhAqB3JInLYKAdrRBuyPENcyToHKY9WYmmD/WEn0hBAmCxzgZN0eEGf2hpRNwjjz6JrQ0r3ARcRSmjzBA42cM3A94Q/BQ1ywhlHARcI/gA3yCS6whBArjJA8hSdRLLCTGCBz6J3EfRDZF4+TZmG0QEpSHVDoYQMGAVnr06CHiCgHVLIFrMxPuU+YKNMDuZQSc45lCu7gsoUWUAGgTYU4Zz525hcWNEEHRYD7xTIEfUsBAaWBgkwRB1BGMBE2ULSVhwBA+jB3mjwbUpUsXYfKaJYif0NSEeaYcGGj5HAtLwDnllFNEaGr6wIUEMyNWP3AIAIcwIs/M1LNnTx+ml5DNaLtE+YRZAxumSwx/Qvnii6Uu+SSYKsyTyI+E2Ea7YpJxL5STgMN4zMwH0SP8NpFWEVKHHXaYHyP3hYYHHKKNIgjQ+MlDKyTKKdtnMgbCDcO4sVjQ6GDYCCnwglZPO9w3HBFOhKimf8aCS4t82jP56Q9NEquEsbPHLUyDHbGwWkgwDoS0WeI5AAcYCB+EEHDIC6l0MGCWwDMCHOaNEICxw/ih46gXs4RCdfjhhwsLGRpCseI5HnXUUT7SLVF5oV9ojXY8N6KPYi3znKFNXJeUhRQwUFoYiG0OIJgVRG5mgjEnMxTM1uRrNGw0ZdwWMEqYG0RcVH9m5tcVIHwiblIX5swCMH2xSQwbdNAHddCU6IPE5IvgUk5CaJmZYIrUjcqpa2ZiXDBtYLEeEDHeqF50hAEDg2uOaGNm5gUEbdDi0MLZfYrNZshjTNwDESeBD97MTGh0XMMcYMisZ2AFMNHPOeccMWYWqtHwsUrMEm+OmCUYDWMALvDohz0KIryDI8qxQGAijIE64JF8M/MuOe5f4VeqGDAzIbAR6lif7CMB4yacOto9z8EsQYusn7FvBqGpoRfaITCgLVyduPcogy7MEs8fxQHBzgIxQgA6KdUbCMAqPAZiJWEApkrKXy/Ki44wH9wOo0eP9h8twKDQbMzM++SZEBA9cKI2nEcJxoVbBTcKbicmD8wNDRrmhhYPfODSnkRb6mFp4KYhj2s03379+onFa/zzuJRgwpQz4dDAmEzEf0ewIXBg0MDLn2hDIp9jlGCoZiYW0hEqwI8WrZnsI0aMEPeDewy3D+UwfqwAJjPaIG2BxxoLbREmrAUwHlxD9IkgAj6Ja+qTonOEDcIJnMGEcHvhrsMVhAYJ/qgLfoFL31yHVHoYgBZ4Trh62CSJdRo2NzrxxBPFc8ZdicuH58RzZX8KhD2uUOgBWsQywN2J2483xLBigUuCnrEweb4IBYR7RAOldxcBUkXGQLGCACKEacI8YbC4M2CYZiaIEyYEIZPQ+hEGn376qfB54iphpyXcLjBDtBwYO8jGJQO8ZGJmIqEpwcjQ1NnQhnpMJiYJjBWTGkHAeOiT8TEO6uGbhcnRNwtwe+65px5//HEhQA4++GBvwaBlwfhZrMPFxCI0ggs3Elo2YyMxMZm0ZiasEASRmYn23DN1wAvaOa4itPfnn39e9AuOGM8555zjF9ARAoyR++V47rnnisnOhj/UZcLjVmPcjIfJjruLLTW5P/AIzhkvDAAGz3gYA8wBawe3Ai4l2iNUTj/9dDEGzlnDoC5jRBBj/XAdUuligGcDzeC6I+E65fmmp6dr0KBBfhEZuoWuoW8EBvSE2xG6YIMkrEHmDy5SFAJoBjpjbiBYmEPQBbQPrSfPn9K9mwBtW2NgR4MfK25AaJFnnnmmIEIYDr57iBBGBAFDlDAyGCmTAEbE2z3HHnusIGQYHBo5CR87b0UwSWBUwEvum77ohx3B0KzQfMhDEMBkeTsIf3vv3r3FegBvVsAoYeDAxW/OzlAIi2h8aMm0AxYTh/ZMRsbNGz4sKNMXrploLEwuJhywEGzAZTICk7UR2pqZuAe0PdYv7rvvPmF1cO/gCIbLgjc7mzHxaQ9+uB+EHXiib96mAg+M6eKLL/ZvMCG8wCfMnzHh/sGSwBqCIZx33nmCyTNO3lRirAg3+uaNInzP9EE5u6YhVDjHhcUaDvDAG7BDKl0MgFcSUBH40DO4h4ahV54/z5J1K54VtAJdkt+2bVuxiMxc4c0hYFDGojLPF9dotP4DfTDHUIqoF1LAwNZioFhBAHC0ErQdM/Pv85sl/NXkm5l/JZNyuR+MkxSVMSm4JpmZf3ffzHwbJf1oD7OE0VGXayZQVAUmDEzyYZAcSVE5ZeRzJD9qCzzyGYeZ+fHThnLqpTttjTaUkx8lysjnmvGYJe45+Zxys4QPlz4YI/U5h7EDE+sGVxfCgjUSJi59U4exUZ9rkpn5dQf64JpEOf3wqiHrCriXKDdLjAc4ZokxUDe6n+S2tOcjJFxLEYOhbkjbFgM8G+iIXjiSOOfZcM7zNzNBJ+RDG7SJ6Ig8M8ubK7Th2cv9OOe5utPwP2CgVDAQ2xwoEHFx9Skn5a9TWF5UhzImBRoU7g0mRFQWHakTnW/OkXakqE3yOXn5r8nLnzalDm2iehwRBGhyuH94RRRXFHnUixL1ovPoSB4puubINdYEGiAMgGvyC0uFlZGHS443l7A+uC6sbcjbBhgoBmRRz6GofEAVV0Z5SAEDW4qBQgXB70FwMLktvYkdsR33g4ZHMkto8FszTuCZbTkc2m9N/6FtwEDAQPnFQAFBAMPABIWBhZTi3VkBDwEPgQYCDZR1GoCvF+VSLCAIcB/w+iNvPYTUUiXjINQJOAo0EGigbNAAbxoWJgwKCAKkBv7skKr5j9ECHgIeAg0EGigvNMBblmYFXcwFBEH59YKFOwsYCBgIGNg6DJTX1kEQlNcnG+4rYCBgIGBgEzFQrCDg7SE+jecT+SgRtoCAWnzpShC4TexHwCIUBJ/Yb2qbTa0HTMZDH/nbkEegOd7Dz1+W/zq6r/z5v+c1OOY7gC0dA22BsaXtQ7vNxwAfiBFLCtqLWhPegzlEfvK3AwQNJJ/E19/JbWgLLEKREK4FOiePRD3yiWaa/Hypz5wltAjn1A0pYKAkDBQrCPiAiTARfCEZJcI1EMOHL3YJYVBSB1E5xMoXwIRcjvK25shEYG8EJghhFHhfnz7yw+QrX2L+9O/WyOqAAAAQAElEQVTf3wuj/OXJ10SP/Oc//1liveQ22/qc+DPsurYl/YAj2rIhTWHfZ2wJzArRZitvErrkK/YI5zBrosMyh/gynhAjPBtiQp122mn+y33KaAO9Rt3DyIF1yCGHiAi7ROYlwCHl0AVB6whrQsRSPhgkEeq9V69evj5fsCcLD9qFFDBQGAaKFQQ04EtHGCkMnIiKxOcnvs4111zjA2pBaGidECgJAqcdTJmYKmg5nEd5kTbENQnipT0aU3JdytDQsSKAy6SiLfXQ8NGkCKdAyGdCVTMePkyjDvXRvGjDeBBonAOTc8ZFHcrIixIhGYgAyQRkQmJFUJc23ANjYUzUpy2bxpDHeOiXfNrSN/DJBz/kc5/cH2Wck0eKYHBfXCcn7pH9F4jjRL/AwvKhbnJ/9AVsxpjc3szEG2DEaUJLTC4L56WPAeiFzZsIP85zNktEnCWcBM8BhQVFithZPCueGV+co6RQRrA64ldFI+NZU5eIpEThJXAjQh3lhzDkN998swhV8sMPP/iNbBAsRDwlbApftBPIENgRvHAMGCgKAyUKAr4rINYJMYZIBMgyM0GgMCoEw6233irioFx99dVioxUYE3F+rrrqKpHYpQzGWtgg2NcVjQbiJa4Pm6rAQImtw8QgHg+aEhMFuOQRJ4hJQEA7YvwwSdCcYLAEn7v++uuF9UHAN/o0Mx8RlTE89dRTAiZ1aEd5lLBwsArY4IYIkcT9oS67oTHhLrzwQrHhDOPgPhkDk5QNawhuBxwC7BEDiDgy4ANGDrMn3ASwyGNcjAXTns1lgAEsAuABI0rsjhbFSaJ/rDDiHf39738XexsgjBgvfQEDwcjYovYceV7ghfFyHdK2w0AkrPv06ePDhdAT84evwom1xTNDsYhe4WP+IBBg8NAcioyZ0cwnni+xoRAihGBBaNAHO+XxFT5frxPDiI1wsPyAB70gbIg3RZwp+oyUBg80/AkYKAQDxQoCM/Nb5MF4IDoSjBetFwZOHHUiJsKQCYSFRsSG3GjJMDk2qSFaJrtnEU/dbAORR2NhYhBxk0iNBHIjPC8hpAkrzQccWB/UQfDA0BA8BH7DpGZCEYCOCQMTRWMmrAMTB/MY5oclYGY+Fj9ME+Z/0kknqUePHiICJGseWv9jjNwX2hx1YaLAR6ihnRFRFMbOxAU2wd969uwp2nAPjBkBQh7xgbgXYNIGd9oZZ5yhI444QggrgtKxX8O3334rwj9w/0zw9UMROAYPTGzy6BM4RBoFH7RDy0cDxW0AbO4N1wD1o4SGSQRVxhjlheO2wQCRYnGlEnAx6gEaJjAgzwGrGgUHZg5zhmaxaNHcUTAIOsccitqigEHjKBrMPerD4BH2CAjeBzczcQ59AhdhQgIG4wEefXEdUsBAURgoVhDAYHnvFCJE68RtAnMHGJqOWSKQG8yaeDowZ4QDBAixIjQIq4tQgInTLn8CDkyTKKcwaELwoh3BvPClwlgJ4ws8xkP0UqI5wmyJwUMEUCYCcOiHOsQtIqE9M1nII2EmY0bjO33iiSfEBGRyRmMCBomJAwPmnnAXMZHR6uiXc7R5Jih+WjR1GDjuLRgxexwQIRTrAaaNIMJagIkTpfSRRx4R5juCBJcWExgrh3JgR2MBX7iAuDfycNEhiIiKisbHGIDBhzz0Rz7WBnlMftqQuB8+EuS5cB3StsMAuOY5QWtmGys9CALcl1i+WJ7QM/MKtxGWJ3TBxjYIimiE0AZuPYQ8FjfRSKFX+onqcIz6MzNv+XKdnM95SAEDxWGgWEFAQwib8MVo/CxaES6X/CiZmTeDIU40ERgv2jH+T3ylhJAm+ibMNWqT/whTI2Em0w7mSThq9hRAa7/hhhv8fgIQOOOhD2AAk7qck+gfBgojxK/PREKzpozEx3Iwapg6WjjMGuuCsvwJTY5+zCwvzAR1zMxvtIOLCcGAKws4wOajExgu/ZNwcdGGMsx6+j3uuOPExIaB0zeCBLeOmQl4MAjamBkHv3DNfTMW7o9M4JmZENIIIPoCd+xny7h5Fsl4oT15tA1p+2IAusYtiNXMs+E5QpM8E3Ycw21I1FgCAyL0KefZUY5iwzW0imVK9FhcpLiJeMsIy5Xnj3XJHENQQD9YjPSLhUoeMLbvXe94vYURFY+BWPHF8owIhptcDyKNiJXzqJwj+TAo3DRouTBy3EcwKsqonwyLc7Sehx56SHfeeafQivB5QsC4VfD941rC3UR7EjAgblwzlFGPvmmDi4U1B9YtWFBjotAHbdCaGRt1yYNRM6k4J5EPfM6jI+3IJ4/EuVnCHGcCvv3228IyYFLijmKc9Is7DQsBpt25c2dvvtMWhoywqlmzpt+gBvzAyHFDsS8C5fSDwMP64Y0TrhkP7TnnCDPBGmLCE9+etQaEZzQG3FLUpR3CqUWLFlyGtJ0wAN3QFXQK/qEJ1niwRtmJDqaPxYeFwLNjLY18njnuTxZ+oU2eH2/EYU2yZoRyxBtG0AtWOutlvCqKoID2zUzUJx93INa2WUKpYDwhBQwUhoFiBQFEjLsHN0hyYzQY3B4QLdYCfkvKYWS9e/cWr7VxhOGxmMXCLUSNJowWTN0omZloh4YMk2ZhuHv37urTp49wnaBFcQ4TR2tiHQHfKflo0lgojAP3FMwT9xUTionG4iwTZr/99hM7czEWNoDhvrgH/LmsYURj4e0cXEEtWrQQ2hf1gINfH3cX9dj4hsnIRjC0ZUyMg4lIHdwzuJXwE4MbGAL1L7roIpp70x149MFmI4wbPGExIMC4NyoiEMAhmiSMH3zjOjIzde7cWeCculhL4I575/VCxkf7KKFxYmUhNKK8cNy2GOB5nHzyyf5Z81xwAbGRE7QADUILWHfMB1yh0BkuV+YJ9ITixPoPcwZ3ErSL4MfdiUXJbnZY29BVw4YNBc0zr6A/Xrigfk2naLBADR1u27sN0MsDBooVBDBbmBGMNvlmIWIIFyKEGNFQKOdNBsxYmCcCBCbF+9P42mGOTA6256NulJgcEC11eJOH96LplwVfJgZEzkRiAlCPdQRcMLSnX5gwggfXFe3oB2aPtsTY0MiBiekMc6UeZWhgaEtMQmCRYMa4v5hUMFTqw2zJ456og3+esSGkGC9wWN8AHzBtND/aYSGwuIcrCmaAcKM+94PWjs+YSQ9OwBNCiQlMH1FCGGFZ8bog8BF6lNE3gsDM/Cu8CDxgI2AQpmiGCDTqwlAQeuCF63KddpCbA9fQK3TAkFijgW7x87POw/qXmYl8BAH5F1xwgVBaoEfWvlAyOIcmUTqgEVyL0CMwoVHyUTygY/oioVSRDz1A/8CgfkgBA8VhoFhBQEOYq1lB0xICMzP/Ng4EKPfjSL479TsrwYS5JlFWGCyYOxoUzJL6Zhv64ppEe5JZwl9vlqhjZn7XMcpI9Gtm3qdPO65JlNE/52bm2ySXk0+iDnXNzI9f7mdm/tws0Sfl1DPbAIc8Elo3fn4WAFmUZqE70sjMNtSnvdb/aMdYwM36rLwDwgJ4aPS0IVHIkcS5meXdr1lijGbmx4yQZZ0EVxtapMJvu2DALPFMkjvjeUHjPO/kfK7zP3+sxMgKN0vAoi0wkttCM7Q1Szx3yswS9fPnUxZSwEBRGChREBTVsLTy0ZgxZ9FkSwvm7wWHSY12zjvdrG2g+ZttmKRbMi60PVxIZpsPx8xEW14vNdv89lsy3tBm6zAAs8dS4Lh1kELrgIFNx8DvLgg2fahRzR3/aFa6TNdsy+GZbXnbHR/TYYQBAwEDpYGBAoIAdwILUyFl+Y+6Ah4CHgINBBooLzTAW2iFCY4CggCfMouefPEY0kgFHAQcFEcDfEwY0nBtKxwEuKWHWz42JeQIQi2/MCggCLAIQsr1309sDh7MzL8uuDltQt3Nx/OOhjMm1I42pjCesk9X2/IZQrP5UwFBkL9CuC4ZA5hbhMHgdVEzK7lBqBEwEDAQMLADYaBEQWBmXtPdgcZc5FDMrMiy5AKzTauX3Cb/uVkChpmJL0f5cprP/vmAy2xDWf52JV2bmce32YZj1MasYF5UxtEsUc75jprMrMDQzOWZFcyPKpoVXRbV2dSjmeXhd1PbbG49s0Qfm9uO+mbGIaSAge2KgWIFAb4kQtsSxiAaFSYLn8aTorwd4QgjJhwD4ytsPOTzhS6J9/KpX1i9TckDFmspwKI+13z4hWXANYlzYr5QxvWmJPCMD4+ge1HinuiHRCwh8hE4RJo0SzANMxNhKvgilfbcH/WT+2Q8XNMHY9+ccdGuqEQIDwThpsBjDITggK6S4UFL5Jsl7ocyMxNfXBM+gfsCv2YbyqlDMjPxLLknrpMTY+LDPu6ZfK6J3wMO6Y+80k7ggmdAiAjutzD4ZubjVXHfUTlj4+M/gs4RL8is4L1GdcMxYKC0MVCkIOCdeBgKYQuIsU8QNzMTE45wubwnz2DMzH+8RH2zBPGamde6yCOpkJ+Z+Y/RKDfbuL6ZKfrxPjV1OEZ5HLmO8jky8YjlAlOgjDokM/P9EPGRaKZMToJ9cW9yP+rSnqO79P/NLG/8yfm+0P2BMRG5lMlrlpjUhKomyBcfyLkqoh/iEBF5tDAY1ElOjAHXEl8qEyuGcBMcI9wTz4iwAuQTd4Zw29yDmYk+uCaUMWXErmEDE7PEfSAk2AMBBsyLAEQ/5Zw+zcwPgzFGiQzOKefINcnMNnrWZiY2K4IWwAX1zRLw5H60JY+jWQJPbHKEgDQzD4uPogjLTT7nWv8Df6+88oq4Z6KzEkeJZ2u2AT5VoUcCG4J/+iGPxDn4JH4V+GEc0AC4gU7IZyGSetTf2mRmIuYVMbN4RsQQIt4TeEmGzTVfinNfxNViXAhtNpIhjx3yeO4IQLON7zUZTjgPGChNDBQpCKJOYCJooEw4JiraDloqE5U6aG2Uk09d8tBo0HbIh+ipa7aBqJkM1AEmgdtoxyRHG+KaczPzDBzNDQ0OTc4sAYOJg/YE44UZyv2AyVjoi7xIcKERwngIgIcAoC8+sCJkBEwADQ74TGIHRsCmTZRPv+RHiT5gIISZ4OtN+iO4F19Hw3jQ2s1MhHUgDASRWCONNIJR1JExEzaD+PMwEhggIQlwNxHAjnAUMDLCDVSpUsXvSoVGzl4NhCUg0B3MhFAThNymjHvk3hBc4Jfxgyvui2dGn9wDeAZvCFTKeTbR/XBtZl648TxpBx6BDb5oj+XIswO+mXlBSh64BZ/cM3AQQJybmegLoQp+onzKYI6MlT0X+ECPcOJcJ4dgNksoJXy9zdsQyRYD4+KZI4i5P/olsacDoToQBAQoBEeMnz63NtEn90ooE4QMXwdHz97MPHjGQPRQBBz4B1e0Aw8IAmJq8cwJNYLABg++YfgTMLCNMVCiICCuD/FNIFwImUlHvCDiDUHIxFInDPP5558vtCEYBJu6QNB8MUwMHSYkdaN7AQ51sDaY5DBW9gcg3goBudDuEAZTp07VddddJ2ATPwVG9KyXcgAAEABJREFUAAyYGtf0Szmv95mZZz4wP8L+woRg1PRNQutEu0YbRlPjfoBP7B/6ZZzAhSGxjwLBvsiH6cL4zBLwYZAwVuLFwEToj3LiAhEGgNASZuYFCjGBYLAwOyY8Yy8ugRcECOEgCCBGAvcwfeISodmCF8IPYDnAbBA8tGM3KuLTEKiMeErgnhAV4J17h7EQkRIBzHiIbwP+iGiJUGV3M3BJeO0hQ4aI5wJOwA9aP+PHqiCP50HEU9xT5IN/4jcBj+cKDjmCPxJxbyIN1yyBRyJsAodYUQgycBnhxswEU4XO+PKc+DkEBOQeojocYZTcK/GteNbkmSUEFhYG4T0IQAhscETcK77UJmItOOjZs6e3Smi3tQlhSCiP3r17+wi6WHBc8+zoO4LPMyUAHYEDGRf53CsCBGGGkOCcZwseKQ8pYGBbY6BEQYAVgLaJFYBmh0aI9svA0FRhBmyzCGNGa4UxQdAQP7H6CQeNSwatx8xo5hMWA1oZGh3MFOb65JNP+l3DYNJoo5jOCBzcHkwe8tHU6QeBwWRjMsNIgAFg+k2eQDBCAnTBMPr06eNj/jM+Ji7jY9IhIGB4CCPuE2ZLkDiYGQH3EFTABT79A5MJDhPkvuiPQG8EGmNshLc2MyEYYM4w2qg9/SIsSZwDM0owMzRDmDpB44i4ipAh5ACMHZyhbcJwwRvjRJsGR4TooA8ScBgP44NZEiiPAGRsfoIlhLYMc4aZ82wYL2M866yzBEPHrYRlgpAgoB6aMwIEIcrYHn30URFYDXrg3mkPLIQnlhcCi7r0AW7pGwbHWBgTOCMeE4KHZ07wQnAa4cHMxH2BP7MEzXCP5JklrrlP3HBEeEV4Rm3BKUKGvhgr9aIy8MJ4YcDARjvHYjBLwIzqbekR+MDmXhDcCEhwY5aAb2Y+Ci7zBzwwNhL1EdZ9+/YV9EdocvDIPWzpWH7vdqH/soWBWEnDNTPBVNCwYcxoowgGCB7CZxKyDSPaOnkQNpMWjYa6aHQtWrTwawvJfUHkMBsmM4wJQQAjZhLgUkKTJnw05TAw4vcAF4aExoxmh7UCkwNWsiCgH8ZhlpiAlDPx0ZCBQTlMhT5h3mjRaG8wbSYifRKxlPsk+mN0X2bmvzZGOJKAy7hgprhymMiMHSFGHyTwA8OOxoP2h3BEeOLuAAb1SDApBBDMHl8xbh5wCJNGwOL6oQyGyzjZphIBACNBuJmZt4pghmjxCE4YDs+JBL7oj2dCSGu0bO6V9uAUpso4acfzxA2FYOLZcw9E0EQoIphRAugHeDwLIqMSdA8GSH3GzbMBh8Rc4rmBc7PEW1ZE2kQ4QxtEdwVP4IDEGLhmXJyTB8Pm+XEeJcoYQ3TNEVxBjwhqBDlCCQuH8ZJ41kTsJEQzrhvcYWZG061KZiaUIsaN8GQdjXtGGJhtgM94oaeoM7NEkDgi94Jv2hG5l539uL+oXjgGDGxLDJQoCCBGJg+TCJcKTITJD4PBVMc/TpjoyDVilnjbA38xkxcGxmRjYibfCO2BA3yYGYyDSJvsN0BYabREmBtmP2GlYRgwPpgDTB9GaJZwIZDHBAQ+cClnEuLGQaOmDxLwokmIYEDTZlMc2mPpIJBgjMAwS7h3GKNZYiIDgzJgcI6GDg6Y+LgdsFJgbjBhGCfjgbkjNDgnYWFwj1g4CB5gkU8CJgwSDRohCa7BDVYIFgBaIwwbpos1hWuL+rRjwTa6P/rHvRUxXmDDgHgeZuajr5JHO8bGkfvkyP2BF/rmvgh9zHgoJ59tMcnH1YPgM0vAo62Z+UioCBxwiaDj/nkGCGBwTj2EBc8IGuHIWg/PgDGRqIPWzDNBsKIUgGeYJWXUKSoxfixA6I41AYQaeAI30A/rRJwDl3tHQJYEs6i+kvPBDwII9xnWHvTJveUXXsltonPoAHqBXhkbtMu4zBJ0F9ULx4CBbYWBYgUBTArmgZ86cimgSTK5mbgQMBMNfzM+ZiYsRGxmfoN2BAR7ClAPV0LyhAMGzImJC9OhL9564Y0J3u6AWSB8sAbQgtnoHe2cSY7vGO0YbR7XAu2pz2SCceKHZf0B1wSTkT7w6eOeYL2Avpi4aF6sJ+DXxTWEZovA4Z6pA9KjcXJOgskxbpgsLhP6Y+8C2sIwsYTwP2NtcL/cA0LOzPzXyuCSxWoYPYyVOsAlcU6/wI8S1yxMYx3h48ddxN4NrEUgeIDHpjUICe4HYcpiKFo4zBQ4kRC+4oorBIPknuiLPnmOnHPkmjERsZSFWiwSmCf3wH3CWMljly2ee+fOnf1aCPDMzJ/zDFo4CxBhhbuQ8b744ovCOjBLvOoJPsAVlg0ClLFH/TMGxoxvH1pjDYEFc+4Bqw3BzTOjH+qSGD944hxaY4EZXEEDKBU8XxQL1mwYO9fQK/SJQKU9bbcmMWYsLAQyz4dxQ5fcBy42aAXBE/VBnyTGTR3a0oa2CBSeJeVR/XAMGNiWGChSEECgaJ/4o2EOTGTMac6ZULhOYDQwbxb92JAFps2Eg+AhaBYK8QMDI9LYuRkzE+4AmAFMBNcTO5NFC5Es0AIbNwmMh52ZYOrkAwdfNucc8U3jT4fR0w8Mi52f6BfmjpBiosEQ6IOx81YNVg4Mj/EDH6ZBGxgO5YwJ5sR9Mg7GzcSEcSAUcUEAA2ZCn+CLcoQUkxg84QtHqJBH+yhRjxRdc6Q9b4tgASGkkssRZMDEbcA6BxYSryiyGAoD4p5g/uRzL7ipcOMAEziMBeYIDBgOz5JnxLipB+PmPsCtWeLZ4LoCv7hRgIlmCwxwxDUvAwCHMbA7G/eAAAYe+KEuwpq6jIc6wEBQgUMsC5g1z4y+GBv3AhwS9wwMFq0RGDw7xowFBIPlnHrcHwIDYcj9khcltGqENEoIdAbNoVTQNwoHVizto/pbcwQO603QI/OBucK6DlYQOIeek+8PGkN5YMzcC0IdWmXO8AowNEvZ1owptA0Y2FQMFCkIIGwYBQwUsx5mgRZrZkKjQ0uFUHmjg8mPlovWiuuDScekZ3KimULowIsGZWaCgcBsgUEZzIHFPbQ1mDp5TBwmBK9Nog1GDBKGxbhgHlgJ1IXZRXVgREw0tFISY2I8MFmYCIwDTZq+Yb7AZ+xm5t8ioU+EIOUwEZJZwkynbyY2vn4mMuf0r/U/+kF4cD8sWgIXKyK5zvqqGx0oh8nxNglMcKNCd5GMCxgYApc8V+Rf68SlhRuHe+H+yI8SsMED2jnMimdGHzApnhsMnHvhmrq0A6+nn366evbsKeiAfBgrri+eN3hDUPKsODczXw/hDr0AH+bPeHjWwCQf/IMjrhGwCHFwSDvwTT6J/hgPwoZnGeGQNQfuD1qI6vF8EYa0IS9KjIFnybMgD/j0xa5gjAk6Jb+0Ev0zTxB20GcEl77Ip5w8juAX4cA5ibFBKzwj6nJN3ZACBrYHBooUBHQOgWZlZXmXBoQZTRzOSdSBGaH1UgZjIA+mgqDgmkRe/kS7CAZlnAOHxDl5JOqRB3zGQx5HrsmnPMqL+qKcc8pIwMufxzXtKAcO8LgmkReV05ZEfpRgJjBT3BPJ7SinHXmUUQfhSR5lJSX6oe+i6lHGWLk36ibXow/yKadechnn5EVlnJNHYqy05ch1lLimPjApJ58j1+RHMBhHdE457ThSn/PkuuRRN7kceORRl/L8iXJS1IYj9ZPrJY8hOZ9z6tKGcxLX+cdEfmmlCH7y/ZDHGJP7II+UnEebbTm25L7CecBAMgYKCAIzk8ViW5xi8bh23W037dSixRbD2Jr+t0fb9IwMf48pqamF3qPMhPbbtVs3ZVSuLK63x7hCHzFtbxxgdWCdhBT31nTAw46NB+hVhfxi+fOyVq/SmrlztGbe3JC2Bgfz5wX8bQ3+ylDb1XPnqrykcB/l+VnOUebChcrNycnP9lVAEKyaPEmz33lLs/u+E1LAQaCBEmhgzvt9NfeDkAIOygANvP+u5vf7Utlr1pQsCGo0bqI2Bx2s1j17hRRwEGgg0ECggXJCA216HaydunRTSqVKJQuCWju3VIcTTlKH408IqbziINxXoO1AAxWPBnqfqJZOyU9JTy9ZEJhbKI65RdCQUhVwEHAQaCDQQLmigZSUAkKAjAJrBGSGFDAQMBAwEDBQLjCwSTdRrCBgdXnKN/014rVXNOL1V8Vxcr+vtWbpkmKB56xbpxk//6RR776t2b8O0/wxY5Sbm1tsm21ZSN/rVq+WG8S27CbADhgIGAgYKJMYKFYQ5GTnaMZPP2rxlMlatXChVi1erEn9v9bovu8qC8ZaxC0vnTlDk776UquXLFZuVpayMwuuUhfRdJtkr1owX2M+fE/ZbizbpIMANGAgYCBgoAxjoFhBwH3FK6Wp06mnq9ull6vbJZdp7wsvUlZmptYsWyYshlULF2jRhPFaNmumsp0lkJOdreWzZimtajW1PfIY1WvfXrVattTaFSu0fM4cVzZTS6ZO1rpVKwGv7LVrXd4sLXQwVi1Y4GECh/PMpUu1aOJErV60SGtXrtTiSRO1zAmZbBi6szAy3RgWTZrg4E1x8FZ5eJnLl/t+lkyb6gVYthsrsJZOmaKl06cJ68BXDH8CBsoRBsKtBAxsDQaKFQTmIMOU548erVlDBjs3z1B/tFhMKWlpWjRhgoY8/6zGvveuRr7xmqYP/MFbCovGjNaSiRM09dtvXP0hmvzNAM0ZMVw/PvyAdxcN/98LmvT1V8pxDH3aD99r+Msvavx7ffXrKy9p4fhxWuMsj5HvvqXRH7ynUW++pl9f/p/GfvSBRjtX0+BnntICB3/F3Lka+r/nNPbddzTG5U/48nNlO0E0e9hQDXryMY16+0398uTjmty/n5Y4obDcCZDJ9OnqKPwCBgIGAgYCBvIwUKwgkJMEOU5jnznoJ43/9GMN/e9/tMS5iVoccKBSK1fWhC8+VWpGZTXv2Ut12rXXFMf4s1z9Bnvtrbq7d1bLQw/boIHn5KiSsxI6nHiKWhx8qNPaZyvTae9TvumvWq1aqflBB6ly3ToeBgIC11ODjrtqtzPPlsVTlF6zljqfe77qtuvgrIKZmvHLIK117Zu7sTTYc29nUUzQirlzZE5IVW/SRLue/ke1OvwIzR87WrVatlLN1m3U/oSTFE9NVfgFDAQMBAwEDGzAQLGCwHlflFK5sjqecqq6XXalmu3fQ7k5uarasKGHsGrxIs0eNkS//PcZjXm/r2Pus7R2xXKlpKUrpVKaUtPTJXPSRIl12upNm6pao0aq0qCBYq4822nny+fMdgLlM/389JOa+t23Wjl/vrLWZirFta3durXSa9VWRp26qtmihSrXrqP0OnWU6/5lerfQRA1+7lkNf/UlZ52M16pFCxWLx1WtcRNVqV9fNZo2l+Ip7r9LlSoprVo1ReNRWfyFMQcMBAwEDGwDDBQrCBz7dnzTPENPr1FDu7q1glhqiib3+9oJhBxVdgy6/fEn6LB/3KMD/3azOp10qqrWq++GCVo77cUAABAASURBVKuO3hJyRySKuez1/6NTtPPaO7dSlz9dosPvvlf7XnmtWh1yqOsvw8F37Xz9xDFqIwfLzJTmxtOix4E69I67dPCtd6rzWecIWDlugVuJJpLx3/xlzrosITxor/ALGAgYCBgIGMjDQCzvrLATx3DTq9dQLMmd0v7Y3loxb66WOzdMmyOO8j79n596XMNfe1lYCJaSolhKqnAZybXHMkhJz1BKWiWlOuuCbmKuDuepGRlqvt/+mvDVF86v/7jGfPS+WzfIlqXEValqVVnMHIiYa5fhYKaIX0p6mlIyKqtZt+5au3qVfn7mSQ194b9+HLF4XHHXT0p6OlVdm1QHp4rSa9QQ1smIN15XtnNd+cLwJ2AgYCBgYMfFwHYdWay43mLxuFoddphzszTIq1bN+d/bHnWsMmrVchp4S6+JtzuutzqedIraHnWM0PKrN26snRyD57xu23ZqvOee4ogGD6CqzjW0s9Pm45UqqWnXbtrttD+o3bHHa7cz/uAYfDchfFodfIhS0hEgaWrefV/nUmpMUzXcdXc17LSrqtStp93/cJba9z7RpZPUwfn/ES512+zi+ttLsVhM9NP60MOV4dYX2h5znLc2LCVF4RcwEDAQMBAwsAEDsQ2nBc/MTNWbNFWq09yj0lg87vKaeGZNHu6h+h06qnbLVkpJSyPLafCVVdX56M0x4/QaNVQZ376zLKo6AUAFGDznlMccY67RrJnqd+yk6o2bijwERLVGjRWLx2UuValXL28MGU4AkYCT5nz+9dq1V91d2ib8/y4zvQb91ZYzJXyb6k4oAZP+6u6yi4ep8AsYCBgIGAgYyMNAsYIgr1Y4CRgIGNgmGAhAAwZ2BAwEQbAjPIUwhoCBgIGAgd8RAwUEwbKZMzW531f+Q6zJ/fuFY8BBoIFAA4EGygUNfK2ZPw8q9IWZAoJgyYTxGv/aKxr98oshlRYOApxAS4EGAg38zjQw9pWXNOWTj7RudSIcT7IBUkAQxNzCbm77Tqq0+x4hBRwEGgg0EGignNBAvNPuirdpq1hqpWQZ4M8LCIKMxo3VoGcv1etxQEgBB4EGAg0EGtg8Gthh8VX/wJ6qs3cXxSptgiDIyclRVlZWSAEHgQYCDQQaKEc0sG7dOv88vQmQ708BiyBfuVJTUxWPx/Nn512npKSIRIaZcfDXsVjhoM3MwzRL1PUNSvEPYyEVBZJ7Ka68qHabms99gzOzbXN/mzqOUO/3wwD0BQ0kj4BraC85L/mcctol5xV3npaWpvT0dJkVTmeFwTMzP/egUWAzHmAAi+uQKi4GCufW6/EBYb7//vsaMWJEoQRH+cCBA0XKzMzU4sWLhUXx9ddfa8KECeuhbDiYmRYsWKAPP/xQK1cm9iPYULr1ZxA2fX///feFAqN89OjR6tevn7KzswutszWZZubv+4MPPhD42BpYoe2OjYHiRgcNvvnmm3lVzEzQxODBgwtVqmDMX3zxhb799ltxntewiBM0u2effVb/+Mc/NGfOnAJzExj09/PPP28Eb9WqVXrvvfc0depUn//jjz/q//7v//Too4/6uWtWuFApYhghuxxhoFhBAEH9+uuvmjFjhic2M/MEZGb+Ojc3V5UrV1aVKlUEkb/yyiveGqhataoqOT+U2cb1zUzLly/X8OHDPaM0M0U/M/MwzQo/Us9s4zLySGaJfATTmDFjPDMmn/GbJcrMzI999uzZQhggsFTIz2xDfbMN51Q123ANbPJIZol88ubPn69hw4aJyUpZSBUHA2amJUuWCEHwxhtv5DFcM9PQoUP9tVmCVsw2HKGbkSNHerrl3CxRBubMEudmiSPKzKhRo7wy1bBhwzyrwCxRbpY40t+UKVM8zZsl8nD5QpsobL/99pv+97//ibk6efJkPfzww95tYGZ0G1IFw0CxggBcQHgkNHi0CYj8scceE1o3WjXCYOnSpfruu+/Uv39/ffXVVzTzae3atfr44491//33CyFBPQgdeL7C+j8wTbQTCPypp57SJ598Ihj2Cy+8oP/85z/+3My8QHruuef00EMP+f5oTv9jx47V448/rpdffllr1qzxwogyhAJjBebEiRPzBE3UP8chQ4aIepyPGzfOa2Vo819++aX+9a9/iTEwFjNTpFFxP59//rm3KsxMMP+XXnrJj2uq07Ywy83ChOIZVLQ0adIk1alTR507dxY0An2CA+gL2ofGmDsICpgv1jZKCUrMihUrPA2/+uqrXmGiLeVPPPGEn0OcQ2vMqdWrV6t69epe4frhhx/0wAMP6OmnnxZ0Tjvg0SfMn/L77rtPn376qadZxsGYzj77bPXp00d77LGHt9CZz+SHVPEwUKIgACVm5hksxAuRQozPP/+85s2bJ5gwGjbEZWaCCNE6MFmpj4kMY4T4cTNBnMCMkpl5TQRB8swzz3gT9d1339U999zj4f/yyy9e+6GvW2+9VQgLGDJ1sVYYC4weZk0ZJjZ9oA3ddtttQvgsXLhQjBdrxGwDg6YemhiTh/GjGdEf48d9lZGRISb222+/LSYeQohJyCTD9KYvhAZCgHYIPsbO+KL7C8eKgwGYLnOhffv2OvHEE71lyDyAtrT+Bz2hnECraObQ8bRp07zLCJcl8wlFCDqD5v/73/8KKwPY0DnwoFtoEJAoULiJECbAAR5zgjLSoEGDPO1z/tNPPwlLgDnaoUMHderUSdQnde3a1Vv3lFE3pIqFgU0SBKAEAoH4/vrXv+pvf/ubGjdu7Jm2malGjRpeq+jWrZsOP/xwLwwgzB49eqh3796qVq2aYMYQKrAKS2jyRx11lPdZ7rvvvtpzzz1188036/zzz/duJDR3zFgEBALh+OOP9wJi/Pjxfiz4Ou+44w7RJ5Pmm2++Uf369f14Dj74YK8J4b7iHgrrPzkPhr5s2TLVqlVLhx12mBgXkxEN79BDD1WvXr3UvXt33z/3hLX0l7/8RX//+9+9hoXgS4YXzisGBlA0YOSLFi3y9I7ygILAXDBLKCDMoy5duuiWW27xtN62bVvvEsIqRjOPaBulBPpDoLRp00bQ46xZs5ThlBPoedddd/Vzi7W44447ztMecwCaZ06AcWAicI4++mjfF/N2l112EWMwM+9WuuCCC3T33XcLhQ2LAyFD25AqFgY2WRCAFhg65iiEFB3J5xrmi2lJMjO/aIwp2r9/f++q2Xnnnf2R+oUlCLBRo0aCCUPsDRo08JYC2hRlCIq6det6GPRRu3ZtX5dz1ijMzPtD69Wr511A5M+cOVNYMBA5jJwJyViV9COPsZslXD+022233XTCCScIawGX1muvvebdP4wN1xcWANoV6yO0ZbwwfyYebgHOk7oIpxUAA9AojBQGjpaONczbOFiXMPFkFNSsWdPTKnnMI2jIzLTTTjt5+iYPhYW1OaxorABoEkUIeoXOoFOsUWCgiJHHXKEt8MgnMS7mB+WMh3Iz88IH+Mypvfbayys9CBqzhMCibQVLFfp2SxQEEB7MkxSdgzHOoyNlMD/84/jbuYbwYKTNCDHtNHNcNGhItKGcY3ICHok8jiTqkThv3bq1d0PBlD/77DN99NFHQitq2rSpcAPhynnnnXeEJQCMli1bektlv/320wEHHOAnGWMBFjCpwxHhxtsVwIPJU859zJ0717djkjARqd+uXTu1atVKhxxyiDD/OUcD416ZsLQPriEwVbGSmfmXAwYMGKBTTjlFd955p7BOb7zxRq+s4HqE1khgBisB9w80h6sG65p8mDt1oEGOvGEHbUH7KDK4HJPrICxQfLBUWbvDXckaBIvItEcwoFAxLsqZH7iuyMdFxVoXLqgXX3zRr39hedA3YwmpYmGgWEEAMWGe8q4xmgXaLkcz80wW5o+2gWaMzxENBcJCc0HzOPPMM/PeJoJgIVwIDQ0GYgQ+6DYzoSUBj2tgomVzjhYDPJjuhRde6Be88JXC6I888kg1b95cmMowYIQALiXa41465phj9NZbbwnhgcBAEPA2E8zfLLE2sc8++/jJymTCtcU9Uheh9eSTT/pFaUxrGP/VV1/t1xxYmEZYIBCwTI499li/dkE+42HymRnDD6kCYABaxvrE/QkNQa/QIAwe5or7BponD3RAX6wvYW0edNBBXqmAJkmUQ/vMH2iOeYMPH8UERQS6ZE4wL81MBx54oID373//27sqcV1ifdMf40ARQpDA9FGYgMkcZs7gigI21u11113nlSXmJ2MIqWJhoFhBgDZy8cUXa//99xeMGk0Hxgczveiii7x2jP8chotbh0Wra6+9VmeccYY6d+7s/egQOxoHbfGhw4xh6BAqqEYYIGguv/xyQehc4/Ps2bOndw2xoNWnTx/vz+zp8lj0hblfdtll/tU3hMsRRxwh+uAtn6uuukowZsZ40kknedcQgoA3JJiwTNTTTz/dm+D0xWRlkjCRzj33XL8mwT3C9HlLiv4QOPTDBOPdbbR/1iqY5HI/LBPe2sAFdemllwqcMZldUfi/g2FgWwwHLR265m22hg0bercotAXDhe5OPvlkXXLJJd6SxG2DsgC9QJfQlpnprLPO8u5I5hwWLDSEMMDvj5Jz22236a677tLuu+8urNTrr7/e+/qhVWgOmuRlBtbooFX6Y30LYQBdYy0AgzU+hAFzgfU3XoTgrSSEAmPeFvgJMHd8DBQrCBg+GkJEIJyTR4rOKYsSE4J8EnlcR4kJQD5tKeOYnKIy8igncU6KyiJY0TGqE11zpC75JPokj8Q5sMgncU7inHISdbgmcU5elKgbnUdH+ioun7KQKg4GoAdoJ/mOo7zoiPWMZRDREEfqR+WcAyO6ppwU0WNyeXROeXJKbs95clkEl7YRzKicvJAqJgZKFAQVEy3hrgMGtg0GcF2i8W8b6AFqwMCWYaB8CoItw0VoFTCwzTGAuwd3zjbvKHQQMLAZGChSEJiZfw3TLBzNAg7MAg7Mth4H0dw023pYZgGGWcCB2abjIKK//McCgoA3F1q0aOHfxmFRK6TmARfNAw7CPNhhaSDMz02cn3ynwks9vChQoiDgDR4+MgmprgIOAg4CDQQaKE80UDPpY8ZkYVDAIkguDOflEwO8JcIHSrxBUj7vMNxVwEDAwOZgIAiCzcFWOalLKAS+uSD8QVm5pTDOgIGAgW2HgWIFAe8Z85k7MXaiIfBeMp+6EzKX8yi/NI58TEPkxN9DU0VLJoIpY9jSeyGmDLFmSHzFyZem+XHENeE2CFaXjNct7XNT2vHFNwHRqEv/3CNfXvO1Ktfk88UqoRCIeElcJ54v+SFtHQagZZ439BVBArfQB7F+8tMAc4760XOJ2nCkLm2gLxLRS8kPKWBgazFQrCBACPA1MGEjIFA6g4gJo0uclCiP/NJIfKb/4IMPij5KA97mwICJE4URRrg57ZLrEmvovPPOE4mvNvnKmYinyXWY5HyBesUVV/hAdsllxZ2zmQ9xaYqrU1gZjIhYStE4zBL7OhALn/D6YfM5AAAQAElEQVQFZokAgTzP119/3Yclpi5xaWA8hcEMeZuOAaKREnqE504r4lZhjUEjfJ3P1+gIYcp4VoSN5gt9hDd5yYk4Qtdcc42nL74W5qvgwgRGcptwHjCwKRgoVhDACNg+j4BuaI8AhLBhEmi0pU2Eq1at8ruHlbaAYdwlJd7v5vN7gsiVVLeocrS0jh076pFHHvE7PvEpP0wAIRO1AY9odX/+8599mOwov6Qj4S5gEiXVy1+OMGdjEt4WoIy1AeCgTeIiIs/MfIC9P/zhD37/BZgQ4yQeDuUhbRkGUAyuvPJKEYUXKwy6JhQElifKAMKgb9++Ppgic4nQKTD6gQMH+rDp+XslUFyTJk38JjRsL0koFTPLXy1cBwxsNgaKFQQQJ0HY+AAGxgB0tEtepeNTeTQYmEyfPn38RhwIDNwKCAq2wYPQ0YzxRVOX9lFCkED0EDNxfnCjmCUCwRHf549//KOPJEo7NCHi/RPXiFg/1GUHMSYTMVXYvIOJwQ5l7FNA7BQ0MMYP02MMxD9CqGGiEyYYq4bokDA/gm7BrIEBk2bslPfu3dvvCwvTZCJzX8SHJ7bLDTfc4HdMi+6HI2Ml4BwB8giKR7A62sFYKWc87CJF9FRiv1CfHdCAyRiJI08eDBptkUiWjJlrolUSEwnmAkNgrwbi1Nx+++0ifDAutSjGPfeB+44+EU4E84sEAXVhSGiUxKZhLwXqccRKIUYUbiOC8RF0L9JWqRPSpmOA50iMIOiRPQdoyWt7BCrk2RFDq1evXiJ+FRYwAprAhwRqhNlTP3+CNlFUUFo4Mjfz1wnXAQNbgoFiBQEAiXTI61NE24S5EEIXTRei5hpmAWHy2inMEW2H8NMwZd5bJfgaDGz69OmA8wlmDEOEwRFAC8bGOUG66IdrBA1aMwwOJshkYVMNNCjcGER0hIkS+I0AdpjYaFIEz6I+dRA2BO2qWbOmj/BI4DuEEi4ozG/GRmI7TPphhygECMwXQcOuTUxO4KHV4ToiwByMHc0O4eFvaP0fGC51caeRYKzcHy4YqpiZn/gEKGPc3DNuN5gB/nkCieE6wKIgaB5B+OjHzLz1QBvyYfQwdCJPIjy4F8YCzhGSMBnGAjPCnQQuSVyzqxvjgUFRB8GCEECYgn8YDEyJaLKsK/AsGHtIJWMA/JIQ+GbmNXcCvxEEjtbMGRQEooVSh2i50BKKFTSKArP33nv7Dzmpnz+hEEFfBG1EYEAb9Je/Xhm5DsPcgTBQoiCAORDGFoYAo4WhoPHCzGH+EDFaI5YAbgiYCsKDiIs33XST32UMQQJTju7bzITWg+sJRkYQLuowOWBERDBFW6dvYKPNQvDAph8YFbAIsYtVgcBhHAgitC20fAQF1gDx12GOMH80KhgvE5IwvNSnL+6DfmCyZiYsIKKbMgYiq3JfbLCDln7rrbfqVpeIAskYkhPjJzww4+GjPMZHv7SP6hEmmHy0eTQ6GABCDi0d5o4gAMdYQPTD+BCAuJywiGDi4I1yxs/9I8S4RyJN4oro2bOnYPKMB8HGR4LgkmeGJQIzATZjAx9YLX/605907733+siW1AcWAj557NE9hGNBDIBbIorCpBGqPCOeQcGaEjuYIexRVoiEi2AwM//MCqsf5SG8eW4IDCxmLDqs1ag8HAMGthQDJQoCAKPBwqQIm4vGghYNM2VREbfKqaeeKrRZmDj1zcyHiKZOpA2RHyXyCc973333iZ2X0IYhahgS2j3MEeaMgIDJsbEG2/ux1R6MFDhm5ndVMkv4SGHitIXhMQGZmDB3xo6GjHaNgEBDp3/6MEts10dfwIwSggxmyDXMkPqMDbhmiQlLP2aJvqlHgpnD5AmpjSBh0sJs0eQoJ8GcEWocsWjADwIGN5pZAh6wowROqUN92tOWxP1yzVgpI4EvEvlRIp/xc401gDBkO0+sEPAB7kkIUgQOQh8YZuY1U/qibUglY4AQ1OCVZxbhPH8rhAB0j2KFtUpY9KLqJreF/lCasMZ5TliDZokNcZLrhfOAgS3BQImCAEaCRg5zgCETOTFiDhAnhI2QwK+O+wdmiLmLu4ItHfH/w8yZINEA0epxF+Fzh7BhQpTRF0dSdA5DR6vFCkB7xTVEv9SJEkwTdxS7QsFc6RcNGqaMdg2zREtHcEVjj9qWdGQctMeCYA8CFn9xMeEiy9+WcQwdOlRsU8jCHxo2Lhv2PMhf18z8hvho8owd/OFqwzJCy0OrZK0EgYlVA/5xT4EP+oGZgD/eQEFo0Y6xJvdjlhCW4I7nwt4QWBWsK2BNYPGwHoArLD9OwTlabWFjT+6jpPOKUs4zQUvHJch+GigN+e8dHEMbKE8oNljYvPmTbC0nt+F5IjCwblEGoC2UIWicNR7coFiIyW3CecDAlmCgWEEAE2VTGtwEMIw+ffr43ZAwZfF1sqB42mmn+Z2RcIlQDqOCcULo+NxhargxaBMNEA0cbRSBAQNEu0UrxlXSvXt3cY3VgeaKi4U+8O2j0V588cVCy4ex48cGJswdjR93E8wStwub1eDC4k0gXr1kArJQxwIdzI36ZgnLBasEC6Gnc6lgVSBEcA8Bm/tkDLTD7fLdd9+JcTOG/JocMBkLQoD+0L5xVTFWYJFg2qwzcH+sIbC4zJixdNitCgsEawJLAtcB985iI8wFZgPTYLEawQBjZxy4Ixg/YwQu/ZAYH2PCvUPi2bCATRkJeAg42mA9kRelUaNG+WfNvUd54bj5GGDuwLCZE+AfAQFtodBAIywoo0DB9IHOHIAeeTbQEoIAi41zaBkax73Hs+b1ZOrRLqSAga3BQLGCAC0TJg5zhImynR2EjDnLNQwEHzbMHC0TjRnGg/aJCwntGb8phJ1/kLgg0GppiyaPuYvlAXOEycHY0H5glGixLC6zSMoC8gknnOB3e2IHMuAySZhoMEQsEd7AgaFSBnOlHa4nhAiwiQnP20pMIsx4/LQwVNY0sE54iwchErVn0mENIVC4b4Qamhhjo06UYObslMY9kfADgyszi6oIIcQbTEx44GA1sFiNhs45uGZ8vBEFDMaJNoh7i2sWH7GieHuKawQNzBrmwjnHvM7cSbTAzFtfLHYj5Fy2/29mwmo699xzhYDyme4Pgu77778X6zzg1WWF/1uIAWj6nHPO8Vu7gkt2IuO5RQnLD+XHLEEjzAssC5Ql6BPlBGHNOc+ZXcZoizWX/1lv4RBDs4ABFSsIzEwQr5n5rR3R1MEZRAlz4pxjRkZGXjllED+MHGKmPfUKSwgVrA6OlJtt6M9swzkwETrA4pz69MuRdjB3tG40YOpRh3ySWQIO+ZSTR3nU1sz8Ih1lwOdIGUfq0g+JN4jI5y0pfP4ILJg2daJEPe4nSsCLyqKjWaI/s8TEpw5jY0ycm5n3zXNOvpn5pmYmrhkDGTwL+omuzUzg2yxRnzokBA7uvGgROrovykjJ/XJN4s0vBCJClOuQthwDZuYtXDPzzzV6bjw7Es+UZxD1wPOJnil50BSJcxJltEvOIz+kgIGtwUBsaxoX1hZmhIsIJmJmhVUp9Ty0XDQk+i514OsBov2jpfMmEq9p4hYz2z73t34IW3SAsaBx4irbVABonmilMJ1NbRPqBQwEDJRdDBQQBDnZ2crKXONSZkiZAQdZAQdhHmxMAwEfZRYfa5S9dq2Um1tAYhUQBAvGjdGQ55/VsBefDyngINBAoIFAA+WFBv73vMZ80Ffr1qwuWRCsmr9As375RbOHDAkp4CDQQKCBQAPlhAZmDf5F80eNUs66rJIFQbNu3XXEfffr0LvvCakC4yA8/0D/gQbKFw0c9s/7tO/V16pStaolC4J4WprSa9ZSeo2aIQUcBBoINBBooLzQgOPrlapVl1mBFYHiXx9V+AUMBAwEDAQMlHMMKAiCcv+Eww0GDAQMBAyUgIGCNkJSg7UrV2rq999qxdy5ea8c5WRna+H4cVo0cYJyc3KSahd+Sp0FY8f4NpwXVmvZzBmaP3rURvCou2rRIk344jONeO0VN47vtG7VysKab9e87HXrNPe3EVoxb55ysrKUtWaN1q1erek//ah1q1Zt17GEzgIGAgYCBkoDA8UKgqzVqzTqnbc04fNPtdYJBTpcOX+ehr/+imeGuZsiCJzgmD92rBZOcIKjkPdX5fKWzZqleaNHK8fVpQ/y5jvh8fNTTziBM1GZK1do+o8DNezFF/LG4ev9Hn/ceLN5F9fd+5zhwzTxqy+dkMzxAuH3GE7oM2BgEzEQqgUMFImBYgUBrVIrV/YMevWSJVxq8eTJileqJBaVychctkxTvumvUX3fWS8cssn2VsTYjz7QpAH9HJNcpVhK3OevXDDfC5Yxrmz5nNk+z2IxWTzmz/mTuWKFpn3/nZrs3UV7nnu+9j7/T9rjnHOVmlFZa5Yu8eNZNGmiRr/XV+M+/kjLZ89ysiNXS6ZMdgJllG875dsBynHa+5xfh2ncJx9pxZw5gNbsX4f6cY79+ENvZWRlZvr8THcfkwck7mPW0CGK8hdPnqQxH7yn8Z99qtVLlsjMxKr7ujWrNfW77zR76GAtmTrNLai5RRh3H9nr1mrOiOFubO9q4pef+/Fmu3HMHjbUW0XjXL+zHXwvTHzP4U/AQMBAwMDvi4FYcd3z/VlKeroqVa2q1YsWOs03V/PHjla1Rk0UiyUY+/DXX9Wk/v20dPo0DXnuv5o5eLBWOmY/wlkN80aN1LyRIzRj0CApJ1c5TpMe8vxznhkvdq6lIc89q9VLlzrmuvEwVrm+6Lv5PvsqJS3ND7FK3brqeMqpqlq/gbMSJujXV1/WkmlTNW/UbxrxxmtaNX++Fk2e6M9nOUY74fPP9Muzz2iyE1Kzfh7khM8nToBkaaq7HvnW6w7GRI15v6/GOyGx1rl0fnv7TU1xQmvZ9Oku/13NcYwbi2jE6685Rj9F834brl/d2Nc6N9Dc337TMmfFrF2x3FsoKxcu0LSBA7XOuYnmDh+ukQ7WEje2aQN/0G9vvK7M5ctdvwM09Pn/OmEw3sHv6/qf4O8r/AkYCBgIGPi9MbAxBy5kNGjrVRzzXeF84stnz9baFStVo0lTyeS17GUzp6vLRZdonyuuVocTT3LMtL8WTZiotOo1fF7XP12qRrt3dvVNs50rJcsx3U4nnaZOp5ymyrVra+bPP7kybfTLcRo0/cZSUjbKj1dKU47zyy8YN1aNduusbpderq6XXK4MB2fZrBnKWZelqk5g7H3hRWp58CFa6dY2uvzpEnU69Qyty1yrXCeM5ATYzr0OUffLrlCXiy/T/DFj/PoETL371deq2+VXqs2Rx2iOWwdY6xj7KieUau3cUm2POV4tDz9CcTem3OwcVa5TR82676NGe+ypBh07+XHG4nFvDbRyfXe/7Er1uP4GrXUutSVTJzs5mKMO7r7BVe1d2mqVW//wjUr5TwAXMBAwEDCwuRgoURDIuUKq1q+vVU7Lnzbwe9Vu2Uq4i+horVu8V8afBQAAEABJREFUTatWXekucV3dCQg0edwm6TVryuIxxVJTVcUJEnOuoey167R63lyNdNbC8Bee0xq33pCT5VxJzu+upB/fMOQ4hr98vTuHolznk5/i3D24XeTqV6lXVxaLKe7gV65dx7lyYPQ5ynAMOiUtzeVXUvVmzb1FEUtNUczVA048tZIynOCwWExV69VTSkaGMp1Vkl6jhtIqV3G3a6pcq5brIldpVaqqQ+8Ttcy5w35zYx7/6cda6SwPV8mBMuW4MTEud+H+u2u3xpHrUkat2n5sKc6aquzGk7V6jRtHhqo2aODkUFzpTki6Cq5N+B8wEDAQMPD7YyBW7BDg6k6LTveMMUczh/yieu3aJ5q4fAREttOap37/reaNHqmJX3zmmWu1Ro20fMYMzXbulSjlOIZfxQmU9Pr11Nhp0jsdfKgqN26s6o2biG4cb0/AdX8zatdSrZ13Fu6lGW6ReIFbOB7/2Sea/PVXSq9ZUxluPMCd7/qc8+swLXbrBRk1aopfbgTIHXNcSuTJM3bOszLXaNaQwW68ozTBwUTDr9WypVYvXqwp333jLITRzkoZpIzq1ZXl7m2Fsyoademq5vsfoEXOnbXCCTIzIOUqlpLiBOQC3zZXObJYTAiimb/87OGDl1WufmVnpeRarhtDDg3d/XLH/jT8CRgIGAgY2EIMlF6zYgWBxUzx9DSlpqc7Bt9AaN41mjSRxeOylLhbO6imVs5dMvXbbzToicec2yZbuxx5lGo7V0r9Trtq5FtvaIxbaE1zTDXmtHLyd9rvAE1wAmP4Ky8pFo+rlmPCsXjcafApMs9g5fJTtNN++6vJXnv79gMfeVCz3SJvJ7dGUKdVa9XvuKvSHeP/6fHH9Jvz99dp01Y1dmruYKT6JPczx6RTK1VyZ5I5Bp1SKU0mKebyM93C8FC3nrHILQS3O663ajrLYaf9emjSl1/qp8cf8W6b5q7/1CpVXItcDXfrIOM++VAtDzpYddq08TDMYqrfvqNbqJ6p2cOGKJ7i+nKCp7lb11jn3EGDnnjUrT98rJ16HOjWVBop7iwRxiEzxVNSHYy4wi9gIGAgYGBHwECsuEFk1K6jfa++TmnOldHS+b33u+Y68bZQE8egW/Y62DGzFDXdu6sOuvUOHfXAI+p6yWWq4txAcceAd+7ZS4fefZ8OuuV2dbv4UrU6sJfIb+XgHHLnP3XEvx7QHuecp7Rq1Zy/f3e1PfJoBy9V0a+Sc9O0OuQwHXLn3Trmocd0wF9vUoNdd5fFYt4i2PX0P+johx7VYa6PNkccqdSMytrZjanjiad4EM26ddduZ/zRn9d21sXuZ/xBMeceisXjanXwwa7dvdr/z39RjWbNZC6vyd5ddPDtd+qofz/s1w+qOzdXLB5XhxNO0hH3/VuH/fNf6njiycqoWUvtjjlOdZ1AqOpcPYfccbc6HH+C9jr/Aiecajj3T0N1dWsXwDn49n+oSddu7h6ra68+5zmB0FgxJ4haH3qYmnXp6scW/gQMBAwEDPzeGIgVNwAzUywWk5klkjuX+8GMSe7U/+c8Fo/78+Q/vi1tXHsHKK+I/Fg8qb4rB0ZeheQTylxdM0vO9eOJxeNKbsc5iYpmplg8Lv9z51E+AsNiKYL5OyC+OPpDHRh1/vxYPL4BlqtMvagO58CKcZ+ujP9mJuBQpvU/zs0S98B51H59cTgEDBSFgZAfMLDNMRDb5j3sYB20dO6dGk2b7mCjCsMJGAgYCBj4/TBQQBDw1ktWZqayy2mqUree4s5FVF7vL9xX+aXd8GzDs91aGuDVfLm1zPwip4Ag4M2gfrffov7/uD2k7YiDgO+C9NbP4T+k2xVwEHBQKjRw5636+anH/bdNJQqCnHVZynHWwOrlyxVSwMHvSQNrHA2GtFwBBwEHpUED61auUpbj7Tk5idfYk4VBAYsgvcXOqtf7JNU7rndIAQeBBgINBBoodRr4nXhr7xNV56BDFU9PT5YB/ryAIIinpSm1Rg2l1KgZUsBBoIFAA4EGygkNpHIf1arJv7Xo2f+GPwUEQV4RCwohyS+sBDwEPGwiDfCCcHJKpp8oPzmPc/I55k9F5eevF65zA31uAn3mUiePwW98UrQgcPVouHr1ah9Tx136/+vWrVOm8zP5iy38A4ysrKzNbr1q1SptSbvN7mgzGpj56boZLULV8ogBM9OaNWu0bNmyKGm5W+PIzs52fDpX0G5URj1wYGaenqm3du1amSVoycz8HKM+c8UskU+bkAIGtgUGihQEZiaEwH/+8x/9/PPPvu94PK6hQ4fq008/FQTuMzfzDwsV33zzjUaPHr1ZLREAzz//vG/HODar8TaqbGYaP368n/BmYbJuIzSXCbDQ5Hvvvadbb701L91zzz2ePhYvXqyHHnooL//dd9/1ytXKlSv1+uuv67bbbtOjjz6qOXPmCDjTp0/Xww8/7PNfeOEFzZ8/P09IlAlkhEGWOQwUKwhgvj/99JPeeOMNLVy4ULFYTHPnztXkyZO9lsN1mltTSHeLDxAwd09eamqqKlWqJPK5Jj9KWBkzZ84Uk4Ny2puZUlJS/CSgHm2AwTlwqcfxt99+06JFi3xdyqN86gMnuqYdiXzyKGM8XEf55FFmlmDg9E+iHskskU/9KFFOG9qamccHk5qJCmwSZdRhvHI/8mgHTPLNzN8n58l1onbUVfiVOQyg4HTr1k2nn366/vjHP6pz586aN2+eateuLeh91qxZvoxy6kG/3377rX799Vcdc8wxXul69tlnPdN/6aWXtGLFCp1yyineYvjqq6/8fCtzSAkDLjMYiJU00lq1avkqAwcO9Ecz89qJmXkCjzSX/v37+3II/q233hKaDJoOGrOZ+bLoD8wRgXLvvffq6aef1tKlS8WkwPKAOTJxPvzwQ0/8WCB33nmnPvjgA69FIUiGDRsmGPAtt9yiCRMmaNKkSXrwwQf197//Xd999523VuhjxIgRXqtijEyyGTNm+CEw+f7xj3/orrvuEtqXmen777/3ls4zzzyjxx9/3E9IX3n9HyygL7/8UjfffLPX7mbPni0sG/pjbNwD93rfffcJ2EOGDPECa8qUKfr666/14osv6o477hBjoB24+eWXXzwuEaz//ve/ddNNN3k80Nf6bsOhjGAAQdCyZUvts88+ateunXj+Z599tpo2bSrmRPPmzb1w2G233dSqVSuvRAwfPlxHHnmkDj30UF1xxRVeyYKWcSP9+c9/Vo8ePXTUUUd5WkQpKyOoCMMsgxgoURCgqUKoo0aN8gRtZp6IYYQw6MicffXVV4X1AEN87bXXPHNGe4dBL1myxLcxM8/48JHCQJk8MOBPPvnE+0Rh7hA8E2TBggXCAnjkkUe8VoRAgKkiKMaOHauPPvpI1atX924ZhAn1q1atKrSpH374QcD417/+JQQHzP6dd97xVsi4ceP0v//9L0+o4Ppioo4ZM0b0xZjp6/333/eau9wPoTJ48GABw8zEOSY9Wj2aXUZGhhdI//d//yfMffy6Tz75pHcLoBW+/fbbXmhiOSB8wBN+YVwHU6dO9RYXwqBKlSr64osvPO7MzPUc/pclDEDPPHtos2HDhtpvv/08XaP0wOCvueYaXXfddZ52oXPmBYoWgh8aIpEPjaNYoVhAaxMnThRwzQJNlCV6KEtjLVEQcDO77LKL12xg1DBWM/O++jp16ujGG2/UX/7yFx1xxBEaMGAA1dWhQwddeumlnughbJgcDBo/KZaFmflJcv311+v444/3DLpTp05+kQ2GDGNu06aNYOh77rmnbrjhBl111VV+DEw2M/Oa17XXXusX6BgH54wDMxtLAK0bjepvf/ubmIAdO3b0GvrIkSO95gUTr1y5smDECByY/YEHHujHfNJJJ3nT3N+M+8M9Y+LXr19fTFauEYBdunTRrrvuql69enmmv/vuu/ux/vWvfxXjxqR3zdWkSRNddNFFuvzyy71APO2004TGZ2Z+EZGxwAyAu/POO3sBxzltQyo7GDAzwdxh4Mcdd5xXJHiO0B7X0CH0ifWLImBmXlGJ7hDaRjBgJWB93n///V6hgTajOuH4e2Kg/PZdoiCAkPFvH3bYYZ4oIWI0Ft5ygIGhhePXrlatmsgDVZzTBqZJOcIAK4DFZ+pQhsYU1QUeEwDf6eeff+7XAXbaaSfP5IFFPzVq1PBrDoyH+lgDTBxgA4865FOPPPqrWbOmb8MYGAv90T+aN20532OPPVS3bl1vqdSrV89PXgQE90R9EkwawcJ59+7dvSkfwWA8Zom3PBgr/WBFUc4YaMNYGSPWA/dJPeBTj77OOeccHXLIIR5/CD/6ok/ahlS2MID1yTPFTcQzhBZ5yw66QSCgPFBOPrTKWhm0gDuI+QGdmJlQkm51C89Y440bNxa0A62VLWyE0ZYVDJQoCLgRmCaMG4b1448/ejO1bdu2QrtG0+/Xr5/69u0r/J8QdTLBct6oUSOvDUPYBx98sNeCyAc2iXMz0/777+/fUIKZwpwxrfGv44N//vnnhXsKZh+1oR3aNqYzdRBSuKUQIl27dvVvZJCPGwefLe2YoGj3+HIZC8IAhmy2sdkNbOqTuH9cRjB36mKuo/mZmccFGiD44G0qrCZcXVgD9IE2F8FKPkbnwGHM3NcJJ5wgxo62SJ/0HVLZwQDPFJdpgwYNPONm5Gbm1wtYD0PIv/zyy17ZQDlgvkCfuAMfeOABYXGijOA2ZU0JNyZuxRYtWnhrFnghBQxsCwwUKQggaphT69atvVYNY9p77739GxEw352dC+OSSy7xjJu3ijp37uw1ZTSaZs2aeWKP2pOHtkNCE0Ko1HTaOn1wBJ6ZedcPQgO3EBoQ/WFSw2DxvWOVMIFwBTFh0LhYjMO9hMsJYYHGhasG6wIXD4vOvKoK86c+LhuEBGNmYbhFixbiXpi8jIsx0QdwOQfpjOXYY48V7iDWF4DVwrUDJ2h6uKJg4BdeeKEw6fERs8i31157Ce0vuj+ECH0hLEnt27f3k58x4VbjFUKsF1wD9EnfIW13DGxxh9ALlim0gQIAIGj/9NNPF1o9Lywg+HkbiLmB65J5w8sVKD/QD1bCqaee6i3UV155RVgRWIvACilgYFthoFhBABM799xzvesEpmdm6tOnj3/dLSJktHwWQHlDgvoQPEwTpsekoD6Mm0nCTTBBevbs6QkcocB6AgwejRtNmnb42qP6MHnetvnTn/6kq6++2rdDQMDoqcM4gMcbRP/85z+9oML9gt+fRTp8skw28rAyYLCM7/bbb/dvDSEsGDfrAyQW5dDuYcbcM2Omn3bt2ol75Y0g1iOYtJRRD98/sOkLuIz3xBNP9JMZocb6CYwfAXLGGWd44cDEv/LKK4UA4n5YaL777rv9WgI4BHZIZQsDZqZ9991XKCPRyKEdlBaUJuiTdSxognzojldNmT+sH6CIoKyghLCGRD7zCmUpgheOAQPbAgNFCgI6MzO/OArz5pqEhm64aHkAABAASURBVAOD5xxixl2CFgtD5pojzNzMPCOE4SW3px0wYIyccwQeC8q4SHDXYCIDi3IzE33QBljUh5mTKCeZJerge6cvGDhWCe9iP/XUU/41T5gxcCmjDmMmmSUW7IBHH8DjHqJzrkmMh4lLG+6PMZslvn9gfMCkHuUkswTcZFhmCXyaJXADPDOjmRcOWCKMg758ZvhT5jDA84NGkwfO8yQP+oRuksuifGiKepRx5Jr6lHNNfkgBA9sKA8UKAjotjAiT8zgnUbewVFxZVB8tCC0KUxhBEOVHxwhGdIzyk4+UkcjjiBbGO/+88okwyG9eU4dE/U1N1E9OUTvyks+Tr6P86Jhclv88+TqqH47lBwM8X1L+OyKPlJzPNSk5L5wHDGwrDBQQBGbmX3FEk92eKdKozSxvfWFr+gdeNJGi49bAC23jpfJcAh4DHgMN/H40AF9UIb9Y/jxMV7RpfNchNfA+/ICHgIdAA2WZBsLYoV9c46w3FSYMCggC/PAsfNIwpEBAgQYCDQQaKB80gILPSzObJAjMzLuGqBxSLOAiFnAQ5kGggfJCA2aJl1OU71fAIshXHi4DBsoKBsI4AwYCBrYQA0EQbCHiQrOAgYCBgIHygoESBQFf9BJRNEp8cFWaN89HZcRiid7sKU3YESzg5x83/fGdAf1H9cIxYGBrMQCdsbMYNJcfFjRHSBOOUVlUn/woLxwDBrY3BooVBBAnX/bypSsfaJGInEnMn00daP/+/X20z6Lqf/bZZyLKYmETp7A2m5tHMC9CNxDPJXkCIuD4yIy4LpsLM9QPGCgMA4RCv+yyy0Q8K+JmEfYkojkYPjR42223+f03aE/IEr7cb9GihQ/PQigU8kMKGNjeGIgV1yFEzNeNjz32mN9chQBwbLDBh19oPcRNgfg58sUuYSKIvhhdU8aeAAgONG8SIaYJzIWQoW8+JEO40J5IjFgeyeXUISEoKAcm8Bkbu6bRH0HauKYeET+jPvhQjS80CfdAGAcz8ztBsfENbZic9ItQQGDQnn4YA/nAJEwF8IBLOXm0ZYMZyrgmP6SAAeYFc4Av5Nl3gP0toBP2oWDPDEJGQLMRpoihxTkfPRJTiM2JoD/yQgoY2J4YKFYQMBBCLbBXAIyUdPLJJ/vw0DB3YqcQe5/AbmwwQ3wUYvCwUxg7h3388ccaNGiQmCBMCIK8XXzxxT4SKVE6YcRs2MFGLew6xt4GtGUvA6KZIjgYA4lIjEwk9hcALpo88VmIQcQGLwgPmDJRG2lPbBciPdIHO58RwoJJxmSjDdEgEQBm5gPFMQbao5UxMWH8RH5Ew2PM//3vf/1mNkxkIkWyvwD9EGQOocEYQ6rYGCAKLbGn+Iod5YZXscljcyLeOoFmUKygMzAFfRPUsXPnziJ2FgpGpCBRHlLAwPbCQImCAOZJ6GkYHgwYLYegWDBKBADRQREUaDxs0kLQNWLmEGmRAFxoOkcffbSiXb+Isw4DxWXEjmMwVsJIozURdO6AAw4QCUFA3xEisALYNQxYBICDMROOgiBwxPZBu8JKYccxIn/CvOmTdlE/CAlCAbPJDVFOER7Ax6rATGeCotFRH8GE0CDoF8KHCKOEuWZ3NAQLk5oy+oisCWCFVHExwDwgrPR7770nrGjoBWWDjZ3OP/98v0VlMnZwHzEPCFeOAvSHP/xB0HJynXAeMLA9MFCsIDAz789EUyc07plnnikiiRIxkcFB4KwhoMXA/M877zwR4pmwu7hXCMZGfUI0o2mzGxhbVz788MN+b16YKEG6zBIB2hAchOg96KCD/IRI1rQ5Z6/XqD8sEjR3BA9hnwnjjBsIBg8Dpw80M7Qyxkp7BBpuIqKdEsmUyKe4j8zMfy8g9zMzf070UqwIQltjPeDvZT2Dr/NwUREt9IUXXhCRT6M+FH4VGgPMEegPixPaRtM3M783AZY1ikYyglB8WE9g3YAouAgRlJnkOuE8YGB7YKBYQQDh8pUxjBa/Ou4dLAK+UGNwhKPgCAPGQkCrh+HixyefyIkwWkxg6iIUsBDYkINNZ4jTTzl1SdThSCwSBATnyQmGy5goI247goHJRtx/QlcDC+0dqwMhxYbgLNABz8xEG8x0xorfH1PczEQ7LBP64h4ppy8+xyYkNeNl3wEsEe4dK4RNevjiEvcUk562IW0RBspNIyxbLGTcooSbhu5Rgoq6QRQTrFeEAIpJpCwVVT/kBwxsKwyUKAjoGG0GxkjYZJgqeTB8LAHOIXYY5E033SQYI2/pwEBhvAgJNoFhExbqoE1jLQCnRYsWYrLAiEn484EH7OicaxICILk/1ivQnnD9sOENi9jUYeMYXDe0J747Y+AcmPhtMcUZI7H/cQfhu8XVhSsKIYK7i34QLgitaLy4qTD9cSex5sHaBv3RB5YPYwypYmMAOsMlhBBgrwkUCPYeiLACvZCia1xDWJwIAZQXLFTezIvKwzFgYHthoFhBgEYP84RR5h8QJi0uIPJhtmzWwuuYxLLA14n/HAGCVo52zt7AmMDAwhI466yzfEA3BEavXr3EJEAzAh6aNi4ghAjXJIQGLikz8+4pmDm+VbR2XEm4aGDILFZjKTChcBHhbmInKPqBud96663+9b5ocxrg0p7x8Jos6weUAYPXWhEGCDD65pwdxhgnAoBJziY0HBljSBUbA9AXdAntQf+cR1YumCH/8MMP93t8cE0d1g6gL/Jxc0LPlIUUMLA9MVCiIOAtIRh3/kHBQCkj38z8lousEcBoyWc3LspwAcGcYZwsJvOWEQkmjqBBSLA4jMXQu3dvmngBwaIwgsRnuD/0h+AxM7/hDb56JhH9IaxwYZmZcBWhXd1www3CnYNwYAz0gxVCv5Szmxi+XBh7zZo1hSBACCA4ECoIISwdBBmWzqGHHirymNisMZDHW1IIMO7DDTH8r+AYgF55gYE1NZQhFKRklDCPoCNoiHzKTzjhBLHedMEFF4g5YmYUhRQwsF0xUKwg2K4jCZ0FDAQMBAwEDPwuGAiC4HdBe+g0YKA8YyDcW1nDQBAEZe2JhfEGDAQMBAyUMgYKCAICsU2bNk18ZBXS9ICH6QEHYR4EGigvNEBYHN6gzC9HCggCXoHjPXpe8Qxpqf+gLuChUDwE3CwNeAlzo+zQAEo+vH2TBAGVeGc+pEwFHAQcBBoINFCeaABFP781wHUBi4DMkAIGAgYCBgIGKg4GShQEFQcV4U4DBgIGAgYqJgaKFQSEfyCMLqEczMx/yEV0TuL0JH8qXxLqCNNAjJ+i6hEmgtANRZVvbr6ZiXES5M7M8pqbmYiHxILJ5ow/D0DSiZkJPADLbEMfhMogXlEUxyipSTitABggzhaxp8aMGSPMcLMNtFEBbj/cYhnFQJGCgBg8xPAhWih7BxB/hy9z2V+AcNCsJVAnum+zDQRvZj6Cp1kij5DOhOTV+h/tSGaJchgqAbfWF/u2lEfX+Y+UkcwS7Sk3M9/OzLzAYpV/yJAh/twsUcaXn4yDQHTJggBYUZL7mVkeLK3/mZnPo55Z4pwIqISllvuZmS8HT+ALARrVlfuZmS/X+h9lJDNbnyM/VvJICr8yhQEzE4EL2XOD+EFEv2WeoBiUnRsJI62oGChSEIAQmCWrzDB/IiqamddyWDyB4aHpUw9ix2pAOJiZWJ1GU8aioBzNiPqcm5nQmrAQovqEciAWEeUkytHozTYwSfLNEuGqKSdgHPDNzDNQ+sSqYGzUJVYQQb24B8aHJcB4aRONhXqUob0ziSk3MxFDHljsM4DwMzMfHI8+GTcwgEtsGQKLAQeYtAEPjAG4tGdclJPHPXFO4hwBSDsYP4lz+qCMOiGVHQxAJ8wTjoSYYD+Mfv36CeuQZ1t27iSMtCJioFhBAEKIw0NQNTRpmJuZCWJnoxZ2KDMzwRyJOApDZScxdgcj3hBBtGCaZqbo98ILL4h9Dc444ww9+eSTftevwYMHCy2aCUOkUsqo89VXX/m+aGtmPlw0G9JQTuA3gsohqNDKifbIXgZs8AFTZwMatDOEDVsHAo9EZFL6ASYJgcU4CStNVFH2IWBXMmLLA5MoozBxYHFP9P3EE0/4LS8ZK9FKeYWOXdLon/hDbGyD9UF8eTbDIRbRL7/8IvoBH2iKffr0EX0QBZXxAoOxc1+MhdDf4JkxhrTjYwCaglZQDNiTYM899/Sxg1AOdvzRhxFWdAyUKAjMTAceeKB/ZxxmBsGDNDMr4OqQ+8F0iSb61FNPiSBbaNku2zP04cOH+20hCeRGJFKECQIGhgezxOrAbQPDJJQvzBHhQ3sSGjbw2B2MIHEICjaP4cgY2ZqSCciag5kJmEzOX3/9VQSnYx8BtH2EA/BIaPb0QfRUokG+/vrrwjrBJYbGz/2w1gAMdlYjYB4RVnGbgQvaDxgwwAupl156Seeee64XEuSbWR6OzMzjgH0duGf6QngS3pv2uMbABwHxEBLAB4ZK/xcgbiMMoJRAyxF46A8rL7oOx4CBHRUDJQoCmBGRPYnKCZNEe4UBRjdkZt41wzWaM64QookSaRTNmiihwDAz8cUy1gPbSqJNw9Rh/lE5bhFC9Xbp0kWEhkbDBm5you7IkSP9oq+ZiclHHlFFiVCK1k+kUfLMTLhqgImAIBE5FHiUcyShwTFeJi6biyCwsFxgzLhviDraq1cvffPNN3rrrbe8awt3F20RKvRBpFMsJ9xRaIWURclsA44QUggDhBfbeWLNcM1+CkSmZDvQr7/+2runAhOJMFg2junp6d6FGI0W6w/LMLoOx4CBHRUDJQqCaOAwOuL1v/nmmz4LzQfGDePH/w6Dj4iefBgkbhh8pGbm26D9tm7dWoSlZjMOXCPJTJPtLoGD1o41gOYcMUOEDwIAVw7howlTDeMmMeEYAzuX8bYGVgIdwuyZnAgc3FZYFDB58s0SY6IesMnjnhBc7DKFiwftHoEEXMZN6Gl8v9wfFoKZeY0fQYGQk/sxfiwI4NGOuggrFo8RlOCI0NdYBGj/CC4EFPfQrVs34R4iNDFWAgKCsTmw4f8OjgHoHYUD+uM5o+BA+9DTDj70MLzyjIFNvLcSBQEMjQQDw3fNEeaMBgvjxEWDiwMmyzWME7cKewfgimFSMBYYHVYFTJPNXG655RbhP+ca+GjYbB7DPgbEZsc9gpsImGYJpo3mzgTDjXPnnXcKBk87dg7Db8++BLiAEBbApE+YLjBYt4Cx4yoySyw6My4Sk5j6CCo0esYFQ7733nsFM6YMCwbG/cADD/hXU9lgB5eSmYnd0ljnwIK5+eabvbUCTFxLvDHFPg39+/f3rirwxroLOEKwPPfcc35xHeH30EMPCdcTedWqVfN7PNA3Ywxpx8YAtIBVihLCnMD9yfOH/qCFHXv0YXQVHQNFCgIYENo0WjvMGULneOutt3q3De4imB4aLRu6oCnD1HFv4N8/8cQT9a9//Uu4S9h0BncPm8SwCQcMmR3IWOzdfffdhbbN5jG0v+7Tc93dAAAQAElEQVS664TAQRgAB42fsdA/rp+77rpLaMwwTBar2c2sZ8+eYmH3yCOPFG9swJjZTYz9YNleE98+zBgtnO0oYfaRpo2/H3cRjBehwriATVvuC+sDpo+FwGJx7969PbPmnliXwFJisnNfWBKsXyCoYPgk8mEMjAtBVbVqVSGUuD92peJ+2cwEePfdd5+Az6YmtANf3HtFJ9KycP88J7R/lAWUIJ4rNGeWUGLKwj2EMVZcDBQrCND+YdAwSVCEZoMrh8VUrnntEy0YjRz3BvVhsDBHmB4MEgaOyYzfnvZow+wOhoBhu0vycDnBCDln1yYYKq4fNHQmGH2RKMeXz0SjDxgxC9JRnwglxkIbmDAaGW1g9sBDSNFPu3bt8tY1YLYII/qiDwQPLhq2pmStg2tgMMkZM/eL4KIPdifjvjhnHDBwhAyCiPrcO2OA4SMQsYioCz4RPggI+mb89AE+eSuJrTIRtOQxppDKBgZ4tjxDFAYsYyxR8rb96EMPAQNbh4EiBUEEFmaWTMwwJ/Io5xz3C8coj3zOo3yuaU+d6JwyUnJe8jllJNrRJjlRjzL6IEV1OCefcupzJI9z6lDGNfkk8kmUcc2RaxL1ovpckygnj0R98jiSOI/KaUvimnzOSdTjSB5lnCfDivLJI1GHvJDKFgZ4zjw/nm/ZGnkYbUXGQImCoCIjJ9x7wEDAQMBAecGAWdFuygKCAFcIbhJcJiFV0Q6EgzCWKuF5BHoMNLClNMB6KeuwZgUFQgFBgBDAP80bOiE1UsBBwEGggUAD5YEGWLdkvZQ1yfxWTgFBwIIvC7os2oZUQwEHAQeBBgINbHcaqLFtcM5bi2abYBHklxThOmAgYCBgIGCgfGOggEVQvm833F3AQMBAwEDAQH4MBEGQHyPhOmBgu2EgdBQwsGNgoFhBwPvQxBYizELycAnTQNA0Qk0k55fGOaGbCQVdGrACjICB7YkBvv2Adgl/QkDBKLzK9hxD6CtgYEswUKIgIOIou30lAycgHAG1+HgmOb80zumP+DylASvACBjYnhiYOnWqD3XCHhXsM0EsKZSp7TmG0FfAwJZgoFhBYGY+uqbZxqvMfEZPKAWiaxL4jUiZ7MbEDk0E3UJAMCkIGvf555+LcyYEDJ4IooRZJgooedSlDXWJ6AlMvmUgiN2PP/6oTz75RGw5SXx+lc1fGHUFwcBnn32mrl27ClrmHBrHQqggtx9uswxjoFhBUNR9QeCvvvqqj5l///33C+2HyJnEzmGnMVxH7NiFeUxUUCJtslMTUUcJyvX444/rhhtuELuZISTYiIYIpsACNjFaiMtPfSKBEoyNvQAQGkWNKeQHDPzeGIDuCXZILCniaKEwsZ/F7z2u0H/AQEkY2CJBAEMmod0PHDhQRAF95JFHROA5NHfeuSUgHFE1+ZKNfQnY0Ia9C4gQCsNv2LCh2JTlu+++Ex85IEgwpTknTgsCgXDSRAElABxB28w2tkxKurlQHjCwPTHAmgAWbdQn59tiHS2CH447IAbK6JA2SRCYFWTAZuZdPkTiJOInEUYRBCyYsaaAYHjttddUp04dseEMgoNPo4lcSlhnIpPiGsIFBJMnWiiRPCkHl4Rp5vzyyy8XO53hIkJAUBZSwMCOiAEsAYQBY2MesCERn/VzHVLAwI6MgRIFAcwaH/6oUaOEv5Nz8mDshHRGq2cdgPWBL7/80q8psBsXQoH9CAjHyyfN1I8SCInO2U+AtQGsBjZv+f77732IaExqwvniIsLlxG5l0SSjfUgBAzsaBjp37izolLWtvn37it3pWrVqtaMNM4wnYKAABooVBGbmd9XCbcNmK9dcc43w5aOZo91jDbDvwMMPPyz8+YsWLfKCoF27dn6XrhvcOgBWAfGL2PSFjW0IYcEo+NQZ05lFZ9xB7FhGXWJ6UJ8du5555hkPF8FCjH7cTLQNKWDg98VA4b2zfwW0zIZNTz/9tNgIqUWLFoVXDrkBAzsQBooVBDBqFn2ffPJJsWCLb5/dvtishl3EWA/AHGbXMLalRDDgBmLBjG0eaYNVwPaObChz2223iY1tsBCYJKwh4DpiJzJ2K2MCIVRYXyDRFjgsRp900kleKO1AuAtDCRjYCAMEa2QHPF6OQGGCZnnxYaNK4SJgYAfEQLGCwMyELx/mDSPniKkLs69Xr5534aC1s6MYibUBtnrk9U/WDGjDmxOsCWABkIfP1MxEexaVzcwvFrPbF/XY6Yt8YKBNAYM3MNg2cwfEXxhSwEAeBsxMWL0oQswTFKm8wnASMLADY6BYQVDSuNHmceeguZPeeecdwchLaldGysMwAwYCBgIGKgQGtkoQgCF8+qeffrpY2CXeNXkhBQwEDAQMBAyUHQwUEARzfxuhgY88qB8ffySkgINAA4EGKgYNVITn/OjDGv7aK1q3alUBCVVAEKxdsEDLh/+qRYN/CSngINBAoIFAA+WEBpYMG6LlY0Yre+3akgVBnd12025XXK29rv5zSAEHgQYCDQQaKCc00PnKa9X2D2epUtWqJQuCyrXrqOFuu6vBrruV/dRpV9Vp3Ub1O3Yq+/dSHp7H734P5YCmAw7DXN5CGmjolPy6u+yiWEpKyYKgQI0ynLF68SJN/OpLLZ0+rQzfRRh6wEDAQMDAtsVAgTWC/N3l5uQoNzs7kXKypdzc/FX8da7L9/Xc0Wf8Tn8YR9T1SrfeUadNG2WvW6c8v5gbnx+nuy/qJdfnOqSAgYCBgIGKhoFiBQEMc+Lnn2nYi89r2EsvaPirL2vmkF+UlZm5EZ5ysrI02y1EUG/J5MkblRV3sW71Kq1aML+4KptVtnL+fM0dMcLJqoSwQohN7ve1sjPXKpaa6mEtmTZN4z//VFO++0aZy5Zp7m8jlLVmjS8LfwIGAgYCBioiBooVBDk5uY6x/iqzmCrXrae06jU0yTHW+aNHbYSrzGVLNX3gQKVWraaUypU3KivuYsHYsR5ecXU2tSzLCaeJX32heGqKzMwLgznDf9WyGdO1yrmIXEYeKO5j1uDByspcoxVz52jRxIl5ZeEkYCBgIGCgomGgWEEAMqxSmpr3OEBtjz5WbY85TnVatdZKxzxznYuF8hzndpnpmOrCCeP9+6npbkV6kTsf9tL/NOT5Z7V0xgyqae2qlRrzwXsa8sJzom6m08anfDNAM34epDm/DnNMOVNTnZb+y3+f0bhPP9YaJ1xoOP3HgZrgrJKJjsmvXbFCo9/rq8HP/9dbJmj81CFhiWStXqM6bXbhUqsXL9bCcePU/ICeWjx5otauXOnzM2rX0vLZs9SgUyel16ylWi1aat7okXnlCr+AgYCBsoOBMNJSwUCJgiDbadozBw3S5P79NOGzTzVv1EjVcsLALLFHAU6YHL+GkOP88Jla4hj/ry+/6F0+K+fP09D/PacVzmUz6t13BFNfvXCBRr/zttPE5yona51be8hyQmCNxn38oUa++7aynLtoqhMIYz/8wJVnacq3AzT5m/7uZs2Vv6WZvwzSuuUrNOrNN7Rw/DiXz7JFruaNHaWqjRoptn5FfPbQIarepIma7LW31q1cpRVz5vi6o95+S4ucO8jicWU7IZZRq6bvc82Sxb48/AkYCBgIGKhoGChWEMDqc7KztMBpzNN/GujWAYbK4imegeautwjizvfeeI891WiPPbTb6X9U9caNvQVRr0MnpVWrpjVLlzgmPFtrVyzXvlddo32uulZ7nHueajRvpqbdujvNfDfVbtVGy2bNVLdLLlf3y6/S3hde7ATJAq1bvVop6Rlq3/tEtTr4EOU4gVPJwUSb38VZKBl16ohfrstfu2KlKterx6Xva+H4sapUpaoWOesk2wmX+WNGCQti54MOVocz/qgaTZo5oRF3rqRK7ljJCYOwTuCRF/4EDAQMVDgMxIq7Y7T91MqVtdtZ56jH9Teox19u0M4HHKhZg39xC7BJC8ZOKMBknW7u3S7zRo5UrtP2a7ds5RktjDqeWkmpjjHH4nFVrlNX8UppnjEjUHKcsDEnYNJr1hK/SpWrKF4pVdlr1yklLU2VqlQhW+0c82/QsZMWjB3j3UgznFuJAmDIjSEWS9zOkunTlelcQ6sWzvdjpWyha7PWuYdqNt9Jddu2c6mtUtwYZOb+s6aQA6iQdkgMhEEFDAQMbEsMJDhnkT3kOoae7XzskzV/zOhEctZBPDVVFo/ntXK1RJJMK50bKHttpqo1auyEwmynaa9WatWqynFumCkD+mv2r8M04o3X/Lv9CIeV8+a5OmuU6hj+WLeGMM8JkSkD+kk5OU4AVJYXMLnyR9rnugXsJl27K61adbEuIPeLxeOKp1VS5vLlvp/Fkyap/m67a/czz1Hns/to97PPVTwjQ3Pc4rGrvtF/hFSu5TrLI32j/HARMBAwEDBQUTAQK+5Gzcy5Y7L02ysv6rt77/ZprXPXtDzoEKfpp+Y1NaeJew3f5dTv2FEw14GPPKRVTiuv4tw15vJbH3aEpnzTT4Mff0T48avUq6967doL3/yMn39Uq0MP18oF8/Xj/fdojvPhtzr0CKWkpTkGn6ZYPC76aNKlq2b8+L0GOdiZbuG444knO8jyZTWaNNXiSRNE/vI5s1Vnl7ZOWFTzn1NXrltX9dp30LSBPygnK0vJv3VrVrvxpCqtRo3k7HAeMBAwEDBQYTBQrCCIpaRov2uu0zFPPKPjnvqvT90vvUIw92QMVXVMfffTzlCac+Fk1Kqt/f78Fx335NN+TWD/6/6q2i1bqdbOO+uQO/+pIx5+XDDwVKehp9esqYPvuEsd3BpAjaZNtf+11+vox59Wr7/fojpt2khm2sO5pfy5pJo7tdBBt/1Dxzz5jPa/7i+q2rChy038b9BxV7c2sNIvPO/hrIA6rVsnCtb/bdnzIO17xVWKxePrcxKHxZMnq1qDhkqrWi2REf4GDAQMBAxUMAwUKwjARcy5gVLS0712npKWJjRz8jdKZqKe3FHuF4vHlVLJ1XXXMSdMojacp6S5/NiGbuMOfiwlYV2Yaxd3fcVcGwfG/+c8ak8G1ylpaQUYeprT6Fv0OMC7i6hjtqEP2gEj7trJjYlrEi6sdatWOmuhvSgnL6SAgYCBgIGKhoGNuWUZv/sGnXZVfZfMbJPuJBZPUbNu3ZVRO/H20SY1CpUCBgIGth4DAcIOhYGCgiDXLfu6hdrcMpjQ9is599Smjp36qZWruJXosnvPm3qvoV6Of+Eg4CHgoaLTQGESqIAgmD9mtIY9+x8NfS6kgINAA4EGAg2UFxqAr4/t+47WbcoOZauXLNHssaNdGhPS2ICD2VuFg4C/gL9AAzsMDYwbowVTp4jvtvJbBQUsgrTmO6n2kceo3lHHhhRwEGgg0ECggXJCA3UcX6/Z40DF0tLzywEVEASxSpWUWqOmUqrXCCngINBAoIFAA+WJBqpWkyW9tRlJhAKCgIJcFozXJ67NzK2rJhLXyeW5ubna2uttAXNrxxTauwX03GFSNgAAEABJREFU9TQQcLF5uICeScl449qs+DkU1eFYWNvkvHC+ec8k4GsDvqCv/KlQQZBcadmyZVqwYEFeyszM9EIhuU5x5zEnfcysyCrr1q3TKrd4wYMqslIoCBgoAxgwM2VlZWnx4sVi3pgl6D4nJ0dL3NpbNI9WrFhR4G7MTEuXLtXChQu1du3ajeYYsGjLHDFLwCwAIGQEDGwFBooUBGYJon7ggQd02WWX5aV77rlH06ZN24hQC+vfLEGwQ4YM8RPDLHGdXDclJUXDhg3T+++/7ydQclk4DxgoaxhYs2aN/ve//+mqq67S1VdfrUGDBikej2v+/Pm65ZZb8ubQCy+8oOzs7LzbMzP169fPt7viiiv0+OOPe+WICt9++63+8pe/+LavvPKKzzcrOJeou11S6KRcYqBIQcDdooGgjVxwwQVCANx7772egPv27euPqampIsHQzcyfp6WlKbpGE4J40WawDEiUV3LrEJwzGXbddVcdddRRIp92pKicMYQUMFAWMADdwrR/+eUXXXnllTrssMP02GOPafbs2ZozZ45XnO6++27985//1KmnnuoFBPfFPJgwYYIeeeQRnX/++frrX/+qiRMn6rPPPvMC5NVXX9UBBxzg240fP17ffPONmJe0DSlgoLQwUKwgoBMIvHnz5tpll118Oumkk7wQGDt2rNd+Hn30UX3++eeaPHmyJ/wbb7xRzz77rCfiAQMGaMSIEfrggw80b948ff/997r11lt11113afDgwWISTJ06VUOHDtWoUaP0zDPPeJh33HGHL6f/kAIGygIGUHqg6SOOOEK1atXS3nvvrQsvvFCVK1f2wqBu3bqiDvdSvXp1Lxg4h6lT/6abblLPnj215557ap999vEuouXLl6tJkyY6/vjj1aFDBx155JGaMWOGcKfSNqSAgdLCQImCAKL77bff9NNPP3lGjqYCUa9cudK7dKpUqSII+7XXXhO+z44dO3rXEcy/du3aqlGjhho3bqwxY8YI66BFixb+GjhoSggIhArnb7/9tidyM1Pfvn2Du6i0nvJmwwkNNgcDZub9+tAwys7TTz+tf/3rX4KucQ2Rj8JDPq7Wd9991ytT9IEgqFOnjrp06eIFxejRo4Xm3717d2VkZAhh8NVXX+mHH37Q119/7RUsLGnahhQwUFoYiJUEiIXc559/Ps81hFaD2Yul0LBhQ51xxhmqWbOmB3Peeed5s/fkk0/25nCzZs2ENYGWM2XKFE2fPl0jR47U8OHD9fPPP/sjriUsAwC0a9dOZ511lk444QTvZkIIkR9SwEBZwICZqXPnzvrzn/+sa6+91q9/oQD16NFDF198sXf7/OEPfxDrZiwMmyV8/QgDM/PzAWUJodC+fXvVr19fvXr10nvvvaennnpK6enpPpUFXIQxli0MlCgI0OhZG3jzzTdFwvWDhg/xYvYiEBAOJPz8MHWYO+Uk0GFmXgNCMCA8aH/ggQeqQYMGXguiDqlatWpeALBGAAzyQgoYKAsYgEk3atRIbdu29e6c3XbbTWj6rI/NnTtXLVu29PTepk0bb0HzdpGZefcoVsOPP/4o5tmhhx7qXUHQ/+rVqz2MJ554Qi+++KJoixuJMoVfwEApYQAwJQoCmDmmaJQgYJg++RwBAnNHAECwuIRY4KpXr54neDPz/v4WLVoIBs8RgqYtRM0RWCTOgZd8znVIAQM7MgagV8a31157Cffm66+/LhaGeRWUtTVcPSwS4+78z3/+o6pVqwq3KYvCvIE3btw4b3EjPHg5g3oIBubDF1984dfccCt99NFH3uJA+aK/kAIGSgsDxQoCmHvXrl394ldE7FHHrBNgwpLPGsFpp53mF8ZY+N1pp510zDHH+DeBjnCLZ7iXcPuw6MWiMGsOrCUgQDB/0aKwEphIwGOiYGKjKUX9hWPAwI6MARQkXEAkGDt0fOmll2rnnXf2Gv7uu+/uX4rAwsb1iVKEpcAaGd8NMJc4suDMHEJIULd3795+/YE1hrPPPludOnUKbw3tyIRQRsdWpCCAkGHEMG8YNtfRPaKp4OY57rjjoixv+vKa6XXXXSf8oLShHi6gM888U1gIvAZ3zTXX+HesDznkEP8KXevWrbXvvvt6s5fXSGmDSc06BJMlr4NwEjCwg2MAl83hhx/u1wf4HgA/P/Rcs2ZNoShdf/31Yh2NdTMsbJg/AgJF6OqrrxblUWKdjTkHjEsuucSvO/A2EXNyi9AQGgUMFIOBIgVB1AYz1CyxqBXlcTQz/70A5ySIlrow72RixapggpglYHBOMktcU059jrQHlpl5IaHwCxgoYxgwM7/OBS0zJ6LhQ9/MDfKjPOieFJUxL0j569GG/KhdOAYMlDYGCggCM/MLWBBnSLGAi1jAQZgHgQbKEw2okF8BQZCzNlNZS5Yoa2lIZQ8H4ZmFZxZoINBAMTSwfJnYoS2/LCggCFZNnqQ5772tGW+/GVLAQaCBQAOBBsoJDcx+923N++oLZa9Zk18OqIAgqJRRWdXq1FXtRo1CCjgINBBoINDADkoDm8uja9Svr2q1anl3d35JUEAQNOq8hw7829/V4/obQgo4CDQQaCDQQDmhgQP+eqP2PO9CVapaNb8cUAFBYCwOpqYqFlLAQaCBQAOBBsoXDaSkFBACZBQQBGSGFDAQMLCDYCAMI2BgO2CgREGQvXatstziQpRysrK2w7ASXfAedlZmZl7/jEW5uYnCzfjLKvmmjpt61N8M8KFqwEDAQMBAmcZAsYIge906/fDg/fr8huv15f/dpK9cGnD3HZr6/beCYW7rO1+zZIm+uPEv+uKmG3z//e64TUP/97xWzp2zWV0vmzlDYz/6oPAxO8GyZOpULZ4yRQiZMR+8nzjfrB5C5YCBgIGAgbKLgWIFAbcVS0lVp1NO0z6XX6XuV1ytFgf01NwRI7Rm6VKKfcrNzlbmiuXKdtbDynlztXblSuW4vFUL5mvVooXKzcnx9aI/1F+9aJGWz5nt60b5+Y/AqFKvvvY67wLf/57n9JEpV5MH9Ne61at99czly7TCw1nhr7Oc9UIe1gQZmcuWKrVyFTXZq4ssHvdjoW/arFu1Sgi72b8O1RyX1rm2Tbp2VdUG9Wmqde4+VjjYqxcv9u3IzFy+XGtXrNDKefOUuWwZWcp1wmSNwwd1EV5c+4JN/hMqBgwEDAQM/H4YKEEQmGOeMccYG6hGs2Y+ZdSsJayB3CTmnumEwMSvvtSYD9/Xjw/+W6PefkOT+n2lnx55SIOfekKLJ0/Ku0PazR01UoOefEw/ubpDnvuvls2amVeefGLuIp6WpupNmqpG8+aq3bqNWh58qNY6Bp7pBMDCCRP085OP66eH/q1hzz+n1QiX2bM17ZsBylq9yjPosR9+4ATXcE3/+SchgKZ9/51+fORBDXzgfv368ouu71maN2K4Jn/1heaPHqWZgwY5ATVHS6dP16Cnn/D3M+jxhzXbCQoHUOM++ciP/efHHtZgV77CCb5lM6ZryPPP+nH8/NTjHk6uEw4Kv4CBgIGAgTKAgWIFgTlOjNbb7/Zb9PZ5Z6vvhefq52eeVJ02uyijdu2828t1QmGJY/YcuzqrYdmcOVo4bpy6X3mNKjdo5JjoMFFGg2zn8589ZLDqdeio/f92k+q1a6cVjnmrMMbJANySQK6zAmhL4tWnmFv5XuM08BGvv6qGu3fWgbfeqRotdtbYjz9Ueo3qWrlksVY7LX7Z9Gla4TT3KvXrO+19qesiV4vcONv1PlG7n3Oe5owcoRXz56rx3nur9ZFHqVHnPbRq8SK/JjH+i0+VVq269r/x72reo6fGf/KJsFBWL1qsynXqac+LLtVK18ecX4dp3siRzurI0L5/vVFtjjjSWQtzlePcaow3pICBgIGAgWQM7IjnxQoCeHNqRmV1Ou0M55650FkEzdWsW3e1OOBAxeLxDffjmHWqc7+0OKCnMmrVUp3WbbTT/j2UUaeO6rZt61+/cg54Xz+WmqpaLVtp7vDh+vHRh7Xc+fur1G/gmbSvUMKfLOd+ys3J9sx6xexZmuO0+V+eflLzfhuhRRMnKsWNt5oTPisXLNCsoUNVu1UrpaSlOfgOcE6uajRvrinfDnBrBu9rnXPxmJniqZVcnQzJncvdyzrnEspek6l2xx6v9Oo1vIBIq1FDq5zFgSBquPvuqt64sep32s2NI1M1d9pJqxYu1E/ufqb/9KOqNGgoixWLWoVfwEDAQMDAjoKBErlVzDHueu3aq0WPA7TXBX/SyvnznD99g4afdyNmTjgkwJmZPFOVY//OWpBjrj65a/JrNGuutscep+b77KfMpcs08cvPHUNdQ2mhyWQ+Hy17tmPuFk9xAqe2UipXdgy5iWPInbzAqeuETtyNt2qDBprn3E/zxoxS4732Fj8zc1bBMk13riHWC9of31vpNWu6Abr/TuLl8DaUO9JVLCVFFjMtcRaF3C/TLVpnZ65Ranq6z4+YfIrri/oZzjpqe/SxTvgdIATnxC8+02pnlbim4X/AQMBAwMAOj4EE5y5ymLmKUwaDdMfqjZuo5UGHOj/6j1rutPFZQwdrcv9+TtvOVa5jpLlO43YXysnKlhAArk2uO5KWufqj3n1H+OkXTZqgCZ99ohm/DNJqt5hcpX59rXGLuiPefM0JhEzXasP/lTNnaKBba/j23n/qe+fXnzXkFzXeY09Va9jQCZJ9tWDCOM38eZBn/Bm1agsmXrVhIy137dKcNl/Vaee5blw5bnwpGenKcYvYE53gGfn2W67vRX7ht1KVKt5KmDvyNzdud8+V0lTfua5GvfOWvrnnbg1+7j9ijFgD2c7lk+vuiRHmZGe5+8lxi9VzNP7TjzXDWQNLp01VultHSXEwFH4VFwPhzgMGyhAGihUEsXhczZ0bqHLdenm3VL9jJ+3kXECSqXLtun4hGUa6U48eSqtaTVgQjfbYQ9XdAq/cr3br1mrkXCnUqdG0mWLxuJrvu79aHnyI6rfv4NwvvdXm8CNVqUpV1WzeQrEUL3pcS/lPoVsddbSAV69DBzXt1k3dL7/SM+mY09o7nHCSOp54il9vwMJofehhvl1lp6HvcuTRanfMMYrF46pSr54XGqnOgtjrwovUpEtX3+e+116vGs5V1MC5eFofdoRSq1RxY9tX1Ro3cu6vntr7vAtcXx20y9HHqNPJp8liMTXr2k013MI5HTXecy812HU3Nei0q3Y56hjVdWPcudfB6nTSKUqrXp0qIQUMBAwEDOzwGChWEMD4GjntO71mTUW/eGqqGrlF1epNmqjmTjupnmPmKenpjhnuphSnccOgWSOoXLeub1KjSVPV2rmld+U06dJF5hhzqqvftEs34YNv6IRELCVFadWqqZlbf+DcN3R/UtLS1OrgQ9XumON82vnAXq7exgy2gRNMlDfcdXcxXtdM9FHf5ddsthOXSq9Z0zH0jqK8eqPG2uWIowQTb+gYeJ1WrVWpalXh+qrj1i6wBMFFynIAABAASURBVCrXriN+td1aRzu3TtB0767iHsmr5+63Sr36nKpOm11Uu2VL0R/jaO/qNt93P6U6geIrhD8BAwEDAQNlAAPFCoIyMP7feYih+4CBgIGAgbKPgSAIyv4zDHcQMBAwEDCwVRgoIAjWrVmtVXwRHFLAQ6CBQAOBBvJooDzwxTVLFit62SVZchQQBLN++klf3vhXffn3G0MKOAg0EGgg0EB5oYEbb9CPDz+ktSuWJ8sAf15AEMRr1FC1tu2V7hZ4Q2oZ8BDoINBAoIFyQQOV2+yiyi1aSPEUz/yT/xQQBOlNmqp2r4NV+8BeIeXDQYNDDlO9gw8JeMmHl3JFK+HeAn2XUxqo0/Mg1ezSTbFKlZJlgD8vIAh4xTKWmioLaSMcgLx+336rwcN+9YgM+Ak0Emgg0EBZowHP280880/+U0AQJBdyHovFlJzMCgKh3uYkM5OZbdTEbOPrjQq34sLMCvS1qeDMzFc1My1btkxLlizR0qVL/dFsQ5mvFP4EDDgMmFnefDFL0Ijy/czM1zHbuDyaZ2Yb8qO86Gi2oUzhFzBQShgoVhDk5OTo+++/1zvvvOPTBx98oMmTJyt3fciJLR3D3Llz9dtvv+XBWbdunUaMGKEV64PAbSncqF12drbWrl2reDyuMWPGaNasWVHR+uOmHYYPH67MzMy8ynPmzNHq1as9XDLBw8SJE30dszBBwUlFT1OnTtWHH36oAQMGaM2aNQWUEOjp559/1ttvv+1pExoyM0GzP/zwg5hjkyZN8u2WOMUDWNH8e++99zRs2DBft6LjOdx/6WKgSEFgZsrKytJbb72lzz77TD/99JO++eYb/fvf/9aECRM8oTKUwjQVM/PllJkZ1XwyM89EIXRgImjMzE+YTz/9VAsWLPDtzMxrTLTX+p+Z+TLySOuz/YFrklmizujRo/X1118r1bm3GPPYsWN9v9TxDdwfzqPkLjf6T/7IkSP1rXMFpaSk+H4XLVokJiICq3Llyl6IUTZq1CjR30YAwkWFw4CZCQXniSee0BdffKEXXnhBL7/88kZMG3rv16+fnn/+ecH0H374YQ0dOtTjCsHw9NNP68svv9Qjjzyi2bNne6Xjl19+0Y8//iiEx6uvvurbAcc3Cn8CBkoJA0UKAuCbmWem119/vSBaCLRTp06eMCFGNO4lS5Zo/vz5Qqs3SwiPlStXikR+pPFQd/ny5Z7ZI2DQgOgjSrQHJkwYbZ62uGO4BgbWAm1gyJybWdTUw8RlQ320sGnTpgnmzxiASbvFixd7i4NxmJlWrVrlJy7t6CMPmDuhfybfgQceqLS0NJcjLxT2228/0f+MGTO8cGBcO++8s3799Vc/aX3F8KdCYgC6gmYaNGjglaU77rjDK09TpkzxSg1IgeZg/GeddZYef/xxHX744Z6usCI++ugj/eUvf/HzrFWrVvr444/VokUL3X333V4w3HzzzWrWrJkOO+ywPJoEZkglYyDUKBkDxQoCmsNEcX+g+aINw6AhSBj3k08+qcsuu0xXXnml/u///k/z5s0Tda+++mpdd911Ou+880QdGDJaEnUpe/31170VYLaBmZuZnzBYC7fccouod80113jtCuaOIPrrX/+qq666Sn/+85+9a4n8+++/X5deeqkuv/xynXHGGX5iDRw40E+kvn37ih/WxrXXXqsLLrjAu6DGjx+vv/3tb6IPxokWhsCgrpkJRr9w4UIxIcnnnhnX+eefr6ZNm2rIkCEin9SoUSNRF/eT2Yb7AVZIFQcDKBPQTbt27bz1yRxp3ry5nxMRFqBXrMiddtrJ0z9KFTSE4lKbQIm77OIVjF133VXkMcdoA33dc8892nPPPdWmTRtPexHMcAwYKA0MlCgIYOIvvviiYLgkGGLjxo2FYIAhwpz/+c9/qn79+t43yoTIyMjwTBdmi2kL4x3gfKYIhn/84x9q27atN5nRqJNvwsz0ySefeCvkwgsv1NFHH+2vYbJYES1bthTtW7du7QUOMBE+aF8IgvT0dNH3vvvuqyOPPFLHHXecn3D16tXTTTfdJLR3tLYpTkvD13/KKafoqKOOUqVKlTaaXEw88oDFGLEuEIjggvvEAsCSYOzUqVq1qhcGZkEQgJOKmmDaWAbR/XMOM4+uoSEScwS6io60MzOvCJGPS5O1BLkfdVhPgyaZD5S77PA/YKBUMVCiIKhSpYrQhGHqaP2Ys2jcmLMwdLQUGHTXrl01ffp0z+DReNCM0Hhq1Kgh3EfAoQ7mLi6WatWqFbgRiBxNaPDgwcLX+tprr3lfKWsHMHkYPO3pj7oImD322EMdOnRQly5dhIAAKH1Vr15d9AFD79y5sxhPx44dvQtr991312677aZXXnlFb7zxhhcq0cSjPZOXdkxC3ESMZ9CgQbrhhhuEnxYBiDAxMz950fKoZxYEgX6v3w7QL0oBCks0FOgoci2Sh2AgIQzMzM8VMxNrTlgGJDPzLx8Ay8w8veJO6tWrl2rVqiXoHlghBQyUJgZKFAQQLmYuTBazFGJkIQsGz4IqmgrEidZCGdqMmeURLMwUJo4GjelsZuJtHPz5+W+EupjIuHBguM+7RbWLL77Y+0bNzJvN0WRhXLhpmCRo97zREzFnxsNkg0HTB3Vpx5Ey6qNdsRCOawgNn3syM6p75s6Epi5uIdKDDz6ol156yQuC3r17C8HAeIFLArZvHP5USAxAAyhA0DZKwbhx44Sy1LBhQ0GH0AfMHbpCgUFAsABMWYsWLYSyNGzYMM/4saJ3dmtP0BfzBkUIRQearpDIDTe9zTFQoiCA+G677Tb16dNH55xzjt59913tv//+6tatm3e14GfHjcP6AcwV5ok2bWaecUPwuJKof++99+qiiy4ShM6kSL67qA2umv79+3u/P2sBuHIQLpQzMWjD5DEzv3BGPsICawWhxITDFYQWjzBB24rqc6Q+C74PPfSQ7+PZZ5/1awG4fBg78OvWresXuzHZWfNo0qSJmOS0Bd7ee++tKc69BBwmPUyAe+RI+5AqHgaYJ927d/cvIbBmxZw55JBD/JrS+++/71/DxkKlDpYuc4k3hw4++GBfBzcl62DMDxQPXJvA5AUL6A4FKaLPiofdcMfbGgNFCgKIDqb6hz/8QbzlcOKJJ6p3795+oRYXDe4XhADa+2mnnebfeGBxFQ3oiCOO8G82YAmwgAtjhsFfccUVguBZ8D355JO95k0/CIVjjz1WderUUfv27cUiMfXOPPNMz6zJp33z5s29pbHXXnsJZszbQwcddJDoH3gtnGaF4NnFLbr96U9/EseePXsKlxCaGMKI+riGmKzczx//+EedffbZwsKJkA1T5xqLB80Mdxj3wlhh9lhHp556ql8UxJJAOCA8KI9ghGPFwgDPHjqFrqCpZBrHhYoiAUZ69OjhX2w46aST/FzCrUlb6JsXGmjLvGLOQGsIgOOPP14R/QEjpICB0sZAkYKAjtDAYbowQl5b4wgTJB8ihQHi74e58oYEBI3WQx2ECIk3ICBitPHOnTsLXycuJpKZ0Y03nZkswCOjhWPoaErAZiGW/qgPc6YPGDVaOmbzm2++6b914D1s8oGDBYGgaNeunYAVTSpcXDB2M/NvX3A/CDXWE4BL3xwRTLw6imWCYGNNgrIoUc7aCONh0ZzJHI09qhOOFRMD0As0Dv1B82AB+oE2mTPksXYG7UGblENzZqZoflCXPMqYT9A+c4DrkAIGtgUGihUEdAjxYqJGKSJQykiUk6J8jiTKSJRxJHFOopxEXpTIj84p45rEOfkcSdE5Gj6MngVcrBXeSMI9BEOmXtSWc1LULvmce4rqUR4l6iDAmKws+HEdlUVH2lHGYjULz2YJoRaVh2PFxAC0Am2QIgyQR4quKYP2kvMoI5+UnM85ifKKlsL9bj8MlCgItt9QNr8nJhMaPq+Jsm6BtlVak4YJCYOPBEtho0NLw/opzX4L6yfkBQwEDAQMbEsMFBAEMDdcK2UlMV6YNkiCIZfmuM3Mu62Kg0n/xZWHslT/XUjAQ8BDoIHfnwbgkfDK/KmAIGCxlddA8auHVE8BB9sYB/UC/EBjgQa2Bw3wMgNrTiivJQoCXCHESyF0QkiNFHAQcBBoINBAeaABXmRAGGySIMgvKcJ1wEDAQMBAwED5xkAB19C2ud0ANWAgYCBgIGBgR8VAEAQ76pMJ4woYCBgIGNhOGChWEPB65qOPPupDTRNCmn0JCLi2tWNjIxd2XSI8w9bCitrPnDlTTz31lN+ZLMrbkiPRT4kyyiY0fOHMDmfEgWGTGvCxJTBDm4qBAV5dJl4QEXmZN3xnkv/O+QgSOiUsCsEbk8t5+424V0S7BVZUBm0TpiI5LyrbEY9hTGUPA8UKAj7aggAJoAUBEw6az+AJ/7w1REn8HnYoK01BQNA5ookWNvk25bEwCf/73/+KSTd58mQfR56oqwgG8EAsJfY12BRYoU7FxABKA+HOmS8ENERxIsBhhA1CohCynZ3LiCHEHgNsTQntoWww1wiCyNfqzC9omaCKzDkUJ7Pw0WKEy3AsXQwUKwjoqmbNmn6XJCJvQsD77LOP39wFQiXsNFvyof18//33fmtLIiXCMJkIjz32mA/xDBwInSBw7MzEnga8U2y2gbBpB/wHHnjA70GAVs6EYs9WwkjQD8G4gEV8H/ZIAD7lWClm5uMbmZnfG4CQE+yf8NVXX/mwvrQjQipj/c9//uPHxT2QT0IAsI0gcZKiV6yoTzA6QkoQcgJBQaA56ocUMJAfA2yPSpRQ5gr0yZauMPWoHrvkMWcIvkgdNnQiACNCASHA5k28RkhoFtpA5wSoI0RLlEd+SAEDpY2BWEkA2V4PpvrMM8/4LfMwWwnaRqRPooMSjZSQzLfffrswi7EaCPVAPjuEEYUx2sv1ueeeE1FB2aEMCyPqm5DU7IUMU0erwnQmRC9mMn2gNcG8YeK0Y8tMhAOTCK0LrR2tCnjsK0D4aqwDBA7t2IEMRo+2hdAghhD9EV2UNiT2ZIbhE+eF8NbEViKGEMHACLDHF8RoaIyf+hU+BQQUwAARaYkLBL0Q14p4WDDzqKKZ+Q2QIlpFqYBGsRoOOOAAQct8zR4pKAQyJIgdUX3NNihNEbxwDBgoLQwUKwjMzIfVJVQzJi0aNhvHQOhYADBStHwYPAwTLYiBEfiNncRoh5sF8xbtGmHC1pWErebDNeqS+HaBCKJEOeV9XTQp9i7AdUTMHzapISIjwgGNisnFWNDQL7nkEm+JAMfMvGuHen369PERUYkwylgRBOQTBZLdzEjJY5g6darf2IZ3bNG+mMhMSj6uAzZ1iWbK2LgOKWAgPwagV+gnyoeOcCtG17zDzdwgUi60dNddd3naRTAQgA7r02wDw2deQK9YzxGMcAwY2BYYKFYQoJmglXz00UfelYLGA0NGG8ciQEuGQULwxPzBnQPh8+EC7hWiemLqMkEIp0senzjD7IkqCnxuCrcRVgBCAgs5jXrbAAAQAElEQVQE4gcm5TBkBA+ThImBGc2YYNBMOsqTJwqTinHeeOONPuQ1Agy3E1o+gggrAuFBsDqECv2TaMfYOC8qMaawYFwUdkI+NA59QkvQPGsCzIEIM9Ap4cvZ5AirGWsZq4H9BqI64Rgw8HtgoFhBIMm/hYM7hbdmSLhuzMzH+sdNwiIWfnjesiG8LswUZgkTJzEpmCBMDFxC+OHxh2IWmyW0n3nz5gktn30BCB5He+pH7UEMcMhHyFAf1xM+WXyo1I3qIGCwIoDFRMP6ACbWC4vUXPMGFC4szHLakRA2jInzwhL948LiXgorD3kBA4Se5kWKjz/+WFjDKDgoS1jRuEdRlFgb4MUGXJDMHfbwIJx5SdhjLpRUJ5QHDGwpBooVBGbmA4axmxdvLvA6JQTNZjMsiuHKwW/PmxJoPmg7aOmccyTBOGHebLiBrx5NCMZNnllCEKDVs3MTb1EAD80fxovPPpokWB7AQoMi7DSL0X//+98Fg0f4oG1hemM5MA58/pSzCxSTjTK539133y1cWLiyiP/usvx/hBivtWKR+Ix8fxACuJfYnyFfUbgMGPAYYP8MQqPfeeedYn2MxWCsX+getyRKCnsVcP23v/1NzIETTjjBzzEAmJmok56e7nf3I4/ENRYw5yEFDGwLDBQrCDBZ0bhZBIah8t4z7hs0boiT1ysh6u+++0747NFy2A0MZg/TRiDgjoERszPTG2+8IRZqgYHLBobPTeEyYqEM+GhTuJ7Yyg+Gf+utt1LFb4359NNP+zULGD/M/JtvvvHbZjJOJiBvHTGR2CIQ64NtLnnbiM1nECgsPGPVYMHccsstfjNwD9z9QbAhuJLf8nDZef9Z50Bg4dvNywwnAQNJGOANO5Qi5gMvPrC5EgpKnz59hOKBa5E8rFnmE+toCIMIBIoPbkteVDBLKEmU8SYbShLn2zIF2BUXA8UKAtAC8ULMUeKafJKZ+TDNMGaYaJQXnXNNfTPzGg7n1OWYXId6XFNGisrJI1FuZn5rSNxDvCV0zDHHiFc6eWOJiYLFQDu5n5n5uoyZPDMTP2ABn8Q5eVFiErL+gSVBH1E+R97qwFq44oor/H2QF1LAQGEYgK6gL5LZBrojn/pm5mmT8ihPST/yzBLtomwz820UfgED2wgDJQqCbdTvFoPFxYPFgfnNgjALb7zds8UAkxqyloA1gVsqKdszfzQ63vhIzg/nAQMBAwED5QEDZU4QgHTWATCfeb+aNQPySivB7LEkkuHhBuP1PrS15PwyeR4GHTAQMBAwkA8DBQTB0unTNf6zTzThi89CCjgINBBoINBAeaGBzz/VtB++U/bazHxiQCogCJZNnaIpH32g8e/1DSngINBAoIFAA2WXBjZ6dhM/eE8zvhmgrDWbIAhq79JWHc7qo93OPT+kgINAA4EGAg2UExrodM55an3s8UrJyCjZIqhSv76adOmqJnvtHVLAQaCBQAOBBsoLDezdRfU7dlI8NbVkQVCgRsgIGAgY2KExEAYXMLC1GCiwRpAMcO2qlcrJykrOknJznY9pjXKyszfOd1fUzVqTKMvKdH4oV9dl5/3PXrfOt83LKOIkNycnUS9f+yKqh+yAgYCBgIGAga3AQJGCAGY86asvNGfokI3AIxymuAWHlfPmbZTPxRK30Dz522+0Yu5czfzlZ2XnEyILxo3VpH5fOVmSS/UCadmsWVo5f55WL16kaQN/cO3XFagTMgIGAgYCBgIGShcDRQoCi8VUqVp1jf/yC61bvSqvV14vXTx5klLS0/PyopPVixdr0cQJSqtWTbVbtlIsHo+K/HGlEx4Lx4/3VoXPyPdn6ncDNH/sWFWqUlV1d9nFtU/JV6MCX4ZbDxgIGAgY2EYYKFIQ0F/j3fdQztpMLZ02nUufZg3+RdWaNFG8UqpGvfu2vv/3fRr38UdatwphYTKLKXPFci2aMtm7jzKXL9OI11/V8Fde0rqVK4SAkUyLXfmgJx/XwEce1NwRI9z1FM0cNEiTvvpcc34boUWTJvn2Wc7FNNEJo4EP3q8xH7znXUZrli7RpP5fa9bgn/XTYw9rxqAfXd18LiyFX8BAwEDAQMDApmCgWEGQXquW6nfaVQucSyfXrQmsWbrUMfhJbuW5oyZ8/pkWT56sSpWravpPAzX712FSzHyfmUtcPWcZZDkmPuzFF7Rk2lStdYJicv9+isVjznU0R2M//EBmptysbI39+AOtXDBf8fQ0pVau4oTPOmcZjFGOcy2N/egD5yb6Xmk1a2ru8F81/LVXtHbFSk3/7jtN/PprWUqKxn/6iZZMner7Dn8CBgIGAgbKOAa2+/CLFQSMhtdIV8yZrVWLFmnaD9+pWsPGqtG4qao3aaoazZqpUvVqynFCInP5ciXEgGsVM8Udg17t2qx27qI9zj5Xe517vnY64EBXN0epGRmqtfPO4lVV3mldu3KlMpzQqd2ytX91tUazpk5gxIUgWOzcUB1PPk179jlfe//pYq107qWVTmhUqllDnc86R53/eLZqOTfUutWrFX4BAwEDAQMBA5uPgRIFQZX69ZVapYoWThinmc4ttNP+BwimO3fkb0pJz1CDjp1Up3UbmVtTyN99TnaWZ+gpGemyeFxp1auL37JZM7Vs5kxVqVffM/7KdeqSnW8ROddf5+Q4wVE58QFEpWrVPZwcJ3hSMyr7tQhzcFMrV3brDh5E+BMwEDAQMBAwsJkYiJVUP8UtCtds3lxTBwxQldp1VLdNGx+rYs2ihUp3jJ24FfNHj1LOurWOcSdBy8111kMjz7jHffKRpv7wvXMHvS8Extply5wwWaWM2rXd4vJ4LZ85wzHyXMVSU/zawDqn3efmmlIqpapavXoa/9GHmuvWDYa//KLMCYaqdev5+u5PokNeQrLEafgbMFBWMBDGGTCwo2CgREFgsbhzvbT22nyzfff1jDzDafBNu++raQP6aeJnn6qOEw4pGRnO5ZOuyo5xpzrhke6YfCweV8eTTtGa+fM168cf1KLHAcLCqNu+vWDmY90i8oo5c1WvQwcPd6d99tequbP9wnGVes5KsJjaHHG04qmpGvPGa8pavUqdTj9DWCiV69Z1beIuxZReq5a3TnYUpIZxBAwEDAQMlCUMlCgIuJlqjRpprwv+pEa77yF+sXhczffZV/v+5W/a7/obtOe5F6iFcxnVa9de7Y89zq0dNFergw5RvFIl/xrpXhddqq6XX6W2xxyv9sefoPQaNdXptDPU/Yab1O2Sy9T5zHNUc6cWqt26tfa99nq17HWQdjniKKWkpalqw4ba49zztO/f/q4uDk6NZs2VUbu22h59rFLT030dBEztFi0UfgEDAQMBAwEDm4+B2KY0MTPP1OWOWv+zWMxp4emKO2Ydi8cVS0kReWjv0VHrf+QhFGLxuNfuyaZ+akaGYqmp4jwWd9q9mWPsDqbLow31SLGUVEV1uTazBBx3lPtRl7UCd7rj/Q8jChgIGAgY2MExENvBxxeGFzAQMBAwEDCwjTFQQBDkZGVp3apVWuf88SEFPAQaCDQQaGCTaWCH55tZa9Zo47d6EhKmgCBYOGG8fn3lRY147ZWQAg4CDQQaCDRQTmhg+Csva9wnH2kdwiDB//P+FhAEK+fO1ayfB2nqwB9CCjgINBBoINBAOaGBGT8N1NwRw5W9bm2eAIhOCgiCym3aqOmZ56jR6X8MKeAg0EAhNBDmRuANZZEGGv/xbDU45jjF0zMi/p93LCAI4mnpSq1VW5VCCjgINBBoINBA+aKBGjXFW515EmD9SQFB4PNzc5Wbk+OT3DkpuubINYnzolJUztEc0ChxXVSbwvKpT8pfVlhe/jrhOvEMAx62Px6Ko0/KmA8ceTYcuU5O5FEW0vZ/duUZ59CVY8cF/hcuCNZXI87PihUrlJ2dvT5HMjOtW7dOawpZcND6X1ZWllauXOmvli9frnnz5mn+/Pk+kR+LFdutb8cfMxP9Z2ZmcpmXGBdwOQKLlFdYWicBTsDAVmAAOs9FicoHw8wE7TInoGtod+3atVqwYIGfH8wTypYuXernWr7m4TJgYJtgoEiODIFCkH/+85/18ccfC6ZrlmDMjzzyiPr27euEC0F+Nh6XmWn06NF67rnnvAB59NFHdfzxx+u4447z6ZJLLtHQoUM3blTEVTwe1xNPPKGff/5ZjIdqZqa5bkH7wQcf1KpVqzR27FhNmjQpTBqQE9LvjoHU1FR9//33uv/++/PmTDQoM/O0f9FFF/k5cfvtt2vx4sUaNWqUTjvtND8/mCfMl1tuucXTvBl2QgQhHAMGtg0GihQEZgkCXLRokYYMGeIJFsY8ceJEDRs2TGgxZia0HqwDNCAsBYZJXiQ4UlJSdNVVV+nll1/Wq6++ql69eumjjz7SsmXLPAy0ItrTBphYANE1sICDRQLTJ5+8unXr6rzzzlOVKlW8kPr1118FHNpTDiyuOXIdJdoDP6pHPnWAvXr1xmGsqUNd2lCPBEwSebTD8slfh3ohVUwMQDPvvPOOnnnmGW/JmiXmUIQN5siAAQN08skn+/lA/a+//lrt2rXTf/7zH5+H4tSjRw+dcMIJfm5BZ1H7cNxmGKjwgIsUBBFmatSoIRLWgZnpt99+U/PmzZWenu618MGDB3vt57bbbvMTAMERae/A4Lxp06Zq37692rZtq0MOOURpaWmaPXu2nn32Wa/xIxjGjx/vz9GSHnroIW9VmJnXqgYNGqR77rnHJ7QnmO/AgQP9WH744Qf169dPX3zxhd544w2vRc2ZM8dbLEw0M/PjHDdunP7973/rjjvu8JOO/s1M3377re666y4PG0uF8U6ZMkVPP/20uKcHHnhAI0aM8IIGK+epp57SW2+9pWnTpvnxUwcLCfi05Z5DqpgYQKFAUTjqqKOEZZAfC+Sh7e+zzz6C3lGSGjRo4OdSy5YttfPOOwvabd26tfbff39P+/lhhOuAgW2BgRIFQaVKlYQGPnPmTO/bxBUDwZqZlixZohdeeMELBjQY6rz++utek4kGizaPBfHll196Zo1VgCbPJEAbaty4sXbddVe99957nvDRlurUqaN3333XWwxYGfhLESBMmueff96vOTAOxrXLLrv49kykb775xvtaYcpYHAgcNCrGgLBBgBx55JGqXbu298ci3N58803tvffe6tChgzfRmcyffvqpH8upp54qhNgHH3yghQsXCqEDs+/atas+++wzn3fGGWeIics1YzWz6NbDsYJhAIXpxBNPFLQI3eW/fQRBq1at/DzC3cm6AIIDq5fENcoIggLrO3/7cB0wsK0wUKIggPE1a9ZMMHm0/6pVq6pJkyZ+PFOnTvXaDMwbc/bcc8/1mg5M2Mx8HQgdt9D//d//6eabb/YuJiYLFkXNmjV1xBFHqHr16l7jPuecc8QkQKhEE4N6hx12mA444ADvDmKCRNp8rVq11KhRIzG+jh07+nHBrBkrzJmxMwiOLVq08GsNrDlMCVCmlQAAEABJREFUnz7d10Xzr1+/vo4++midfvrpOvvss/0iN8IAX22XLl2835Z7RmgwloMOOsgLPiwTLIUnn3xSrJcg1P6fvfOA06o6/v5vdpcqvSNIUUGQDhawgMaOJUSjsRGxGysWsCaCPRjzYkVjr/GvkRh7FwRUsKECoqKACiJIkd52ec/3rBcftqI8u+wu83w43HvPPe3+ds7MnDn3ziAY6c/TlomAmQkFp7CnNzNBv6yMhwwZolNPPVVjx46NgoF6rBKYCwiLggSJ/OcIlBACxQoCCBJmicnnxRdfVNeuXdcTOxo3tnU0bRg3zBJCh6iT8VavXl3XXnutYJSk66+/XqwCaJfVhlnu5EFrpw/a4a0K7O9oUMk199lYQ7BQL2mfI/dg9ggYNuq+++47MZmoy30SDBzz0t5hjwIBhk2W9mmPhN2fZblZrjkKYUY97Lrc45l4NhL5PBd9sDog9enTp0BzAGU9bdkIQOsgwDx57rnnBJ0lZkvyEzr95JNPIt1Wq5b/gx/KeXIESgqBIgUBBAyTxZSCVszbOZhxIFwYNXsFmGfuuOMOkR555BHtuuuugklSj/qkZPBmFs1G1CefNjjWCSuDVkFjxy5PwhZPu/QLkx41alS067N3QDlWAUn7mJnYL4D5s+mGeYb+GjZsyCEmyrKPgMkHIYLpiYSJiwJo9bT92GOPxf0LVhiYuBAW9957b1z1IAyT8TJRWS3QLoKBZwAfxsI5bXrachFIpQHOedkCUybKBFo/9MZeE3taXbp0iStilA32nTBFmuWuprdcBMvDk1esMRYqCGDWNWvW1CGHHCKOu+++ezSdwGCxgXbs2DEy/NNOOy3a1yFybOpslGHL79mzZ9y43W233QTzToWNyYE9lbZZVaCZYw5CW+de9+7dRVvUQZPHro9ZBiEzYMAAMQZMRTDhvfbaK/ZPWcYJE2fpDbMmj0Q5TFfkw8zZE2DTDgF08sknq0mTJtHEdMQRR8Q3kTBXUR4MeE7GghkKcxECysxEGRKCh/Hss88+cdlPf562XASgX1a80INZLkM3s/jCAjQJLaFMmZkOPfRQYfaEPqE1aBr6pY0tF0F/8s2BQKGCAGKEscL8IGAYIrZ0iBb7e+fOnaN2z34BG6Z8HwDTpiyCAAFgZvHtBzRv2ksekHMEQb9+/aIGzjW2UQTDWWedJRgyzDmZHGwUDxgwIOYzJtJeQQCg3SNk+vfvH4XSww8/LMw+aOuYipL+OMLAeVeb9hE6aPj0i1Bjb4P20cYoy9h4VsoeeeSRUUgkwgqmz7h4TsZAmeOOOy5uEFLX05aNADQFHcHgzSyCAT2yWoVuoDv21KAbzIlVq1aN8wj6QuFB4aCNWNH/cwRKCYFCBQH9Q5CYPziHiJNz8rkmn3O0bDTj1LykLEfKUDY1kUe91DzK0g753OcebZJPHok87pGfnHOf/QP2INDS0ciS+5QhUYf6edsnP8mjXJJoM8mnDPnUT865po+8Zcj3VGEQ+E0PAo1AK0llaAla4Zp7XEM3SR75JPK5z7knR6A0EShSEJTmQDalLyZUy5Ytxcc4BxxwQFyGb0p7XtcRcAQcgS0JgUIFgZlFhmpWfo7sUyR/PLPyM24zH6uZY2DmGJg5BmYlh0HCH/Me8wkClqYsa81KbjBmZattMx+PmWNg5hiYOQZmFRMDLCeYH/MKAa7zCQJeq+SVNk9d5Bg4Bk4DTgMViQbatGmz/jswBECS8gkCM4uvffLWjacMxyLDMfB5UJFowJ9FBfzyCYICyniWI+AIOAKOQAVGwAVBBf7j+qM5Ao6AI7AxCBQrCNg8xvPm5MmTY1yC6dOnR6+gNI5zN/wDcb6xCYdx7pxtY9Hycr8Rgc1SjY043ETgQZTgTDgvzDsQXKbgqgW3E3z8yIsZSRk28/BAiq+tJM+PjkBpIFCsIMA3ypAhQ3TppZfGdNFFFwnXzfhGwb8/juh+zUDxs8KHX7+mjpd1BMoDAjD3iy++WJdcconOOOOMGNMCp4zJ2PmIDKdzF1xwgQYPHqxzzz1X+MlCAFAGd+1XXHFF9Eia5JHvyREoaQSKFARoLwRmwf3DDTfcIJg47hhwAocrZ4QBxM2qgZXB1KlTxYqBPAaOZoOzLdw9422RPI7UIw8vjORRn1itaFG0mzoJuCYqGnUoS6JdBBSaFRoV9dGkGMO0adNiCEu0MfqmDM9BPU+OQEki8O677wrXKrfffnv0uIuSxHxI+uTr91deeSX60cLpHO5bcO0OfRLsaNiwYWJeJPMnqedHR6CkEShSELB0hZGeeeaZ0bEbPoP4cpd4qvhTMct935aoZfjxwacKjrMIKgNTRuPp0aOHcFiHd1KEAEz78ccfjyEr99tvvxhlDAGCv57EnTP1EQbESiY+Ac7mcF9NfeIV48edsvSHa2nyb7nlFjEG/BcR0QyNizJEekKAIRhKGkxvf8tG4PTTTxc+t/DFhWM5aB06TlCZPXu28FHFh498Bc+r2tTB3xDK1uWXXx7nBfWSOn50BEoDgSIFAfEFIFwINhkMzrEQAuSTB9GiteNtEa0GLQd3z0RggiHjCA4NiVfwsKGyVMapG8wewUL0L/yw40iOKGVoRUQkQwgRzIZrNCvGwKQiCAxurtG+yMe99OjRo7Vo0aIYCpNr6rLCwASFa+mPP/44Rh9jvJ4cgZJCACeI0Dku0QcOHBjDshL5LukPzZ8oeswJ9hJYZT/wwANiVYuTOuJbJGX96AiUJgJFCgLiAaBto90ng2LZ+v7770cvn0kefvhZ0sKkIXAYNkweb4poPvj0px3y0X5YVXTt2jV6JkVTR3uC0V9zzTUilCWuqdlUpn9c9aL5s7qgLkttvJHiUhqNH9fVrFoQULSLZsVEpByxiIcPHy5MS6wkEFrJmP3oCKQbARg6EfJQXvDWy+oAwZD0g6AgH3PrU089JfbeMA3xMkZSxo+FIeD5JYlAkYIAF81o/zBy7JsMBE0cQoZRc41gGDNmjPhibdCgQcK1Lvkwftw0w4xxK/38888L+z0MmwlBGc7NLH7pdvTRR4sVAOaikSNHiv7Q8mHq7Amg3RMtDDe9X3zxhTjn/pQpU0Qey20mHYnVBROOZTfxEog9wHPQpydHoKQQYOX56KOPCnfsuJmGDlP7QtlBySH6HUoJ+1ocodnUcn7uCJQ2AkUKAjRw3hJ6/fXXhfbdvn174bc/+eSapa6ZCVMPmj/xBNDAIXDMO2hGxx9/vLDjw/wJusGmL8KDB0VYcI7t9LLLLov21VtvvVVMmG7duokVBW6lOScfjYs8TEKsKAgwQ/g/9iBol/sIF4QRAuScc84Rb2iMGjUq2mbNjG49OQIlggB0zv4Xb9hBs9AnZkui7rFaJk4HcTyIn9GuXTux18YqlhgXDAihwKqZI9eeHIHSQqBIQcAg0PSxsw8ZMiS+Evfggw8K4cAewQknnCAieUHY//jHP3TeeecJgmdlgLnnoYceioyY1+nuvvvuqLkToalnz540HRk9qwXaYLOXTem//vWvYmOYmAJMFAQLEwtBgMBB08euSh9Dhw4V4yF0JcFxmHw0jKAizOT5558vylGX5+CepzKNQLkdHPtfvPBw9dVXi9UAiZcacI/OCxMwflYDp5xyilhRQ+vMK+aDWa6Cwn0UHZQcs9y8cguID7xcIVCsIDAzwZQhchg9b+EkS1nOsdXXqVMnRg/DFIMWhEaOrb5Vq1ZRy0dgtGjRIrq1ZmWRbIqhHVGeqGfko8FjImJFAIr0QzQxwkkmph3yWJlg8mFM9E19hAt9UI/EOe9yU5fJaOYTC1w8lQwC0CAmyLPPPltJYj4wBxJBQM8oUGwMM5d4G468JGHeRAhQx8zpNcHFjyWPQLGCoOSH4D04Ao6AI+AIbE4ENosg2JwP7H07Ao6AI+AIbIiAC4IN8fArR8ARcAS2OATyCQLeBOK7AU8L5Bg4Bk4Dm0oDXr8s0RCvLvNmWl5Jl08Q8OUvX+XynYCn7+UYOAZOA04DFYEG+KiW77M2ShDwGhzv9/NevqeVcgwcA6cBp4GKQAPwdb7byrsa4DrfioBMT47Ar0TAizsCjkAZR6CoDxWLFQRmFt//N8s9lvFnLdXhmdkG/ZlteL3BTb/YYhAwczrYYv7YFeRBixUELIlwDJck7Et81FWaz09/Zpt/cpmZkqUV7ixwowEeZrljY+mFaQ1syKeMWe498jxtGQiwzwadQLdbxhP7U5Z3BAoVBGa5TA8fQH379hWJhKuJWbNmxVVCaTw8y5kZM2YI/0Wl0V9hfZhZDHiDLxk203FzgSsMrhEACMyrrroqbi7zhSjX+JjBs6SZC4PCcK1I+SgBuFXBbQpftBNngy+OK9Iz+rNUTAQKFQSpj8sn84SlJJmZiBuAxkMZtF7OYdhckzgnHwaJZkweRyYKZTmSR+KcsiSuKUe95JpznMwRvYz7JNog0Q/X1CGRR3vkUZ9rzpNEHu1Rljzqk0dK6lGHxD3KJIlrfMPgboM3CPB8ipMxhBQurwk52KFDB+HagvZwsYErDbymUjdpx48VEwG0f0JVknB0iPsVHDGiwJi5IlAx/+oV56mKFQR488T/CR4+cQ6HtoOZiO8NiEuAAy00Y1xHkwdDHT9+fHSshQtqhAZLZbQjYg0QqQx3vWYmyj7xxBPCIR3RyHi1CWZLewSzIY4Anklp75lnnhGBcsaOHSsEEmVeffVVwdi5TwjAm266STibo/377rtPBP6YPHly/GuRx1jJQ7AwVt7vJUgIEcxoI/Elf+ONN2r06NFKBIaZiXEQ+hLfSLHB8B/v5PIMhNxkrATlQQM0y3028MJNdmmuoMKw/N9mQIBV4KeffhpjbOBRFOdyBFAiSJJZeRQEmwFE73KzIVCsIEC7hZnB9NF6Ydi4zeWdVJgurnXx+gkjhXliNsEDaZMmTYQjuHHjxomoTLiFfvnll2PcArTq5Im5B1PGeR0RzmDYOONiYuE1lHI4nOM+TBdmjTtrYhDA7GHQCBn6ZxxES8MnPOOCKRMABI2dMbVu3VoINOIiEBWNfnGxTQCcGjVqCA2uQYMGwlMkKxAEmJlFMxhCALfcOLlD00fzR4jgZRXzD2NE+4MZ0K7Cj1UB5WEG4dL/VXAE2DPCQy4KBG7XOcdNegV/bH+8CoBAsYKAoDBo0XhSxHUuqwH2CtCAYawnnXSS+vXrJ+IGICxgujB64gejIR9++OExIA1MFQZLHm6jwQ6TCcE70KBoi2U1nkqJSnbiiSdGbR8mi/dQGC4CAHfSBMchJCUmGpguy3IYPG3vtttukZEzJhKCjAhmMP+JEyeKeggBIkOZmZoEgXXwwQeLOLNEWkNYIVQIMcg1Y2SsrFYQgAgX8nEfDCa9e/cWk50xjBgxQq+99poQLggts9xYDawcknZoy1PFRAABkPpkZib/u6ci4udlFYFiBQEaLZo5ZhjML0OHDhXatlnuZjKmGSYAR5ghCSYIA8Vdd6cAABAASURBVCafTVOzXK0aF7wAkTo5MD2h/ZMPk6U89WiPcrTH0cyEto0JiihmF198cQxgk9xDA0vO8etOe1xzpI2qVauKMizXmzVrFl1r0w95ZhYD1xAqEzfYlCc+AiugZGxmtt5URJsk+pk0aZIQbKxEcKWN8GHfAIFJGcZgZpyWxeRjShMC/J1RKlgps8eE8EeJSVyqp6kbb8YRKBEEihUE9AoDRwuGiZrl2r/R7rGNY/MnOAxCAvs5mj3mofvvv1+YTp599tloDoLhIiBoLzWRB0OmbRjpI488Euuxb0C/hMs0M7E3wMQijyU44TGx8bMioD4TkXY50mZyzj3MNowLc07btm0j40YYJGXNTLTJm0CsXFgNwNyTNjhiPuK5EHBcm5mY9JiyCJbTqFGjeI0Q4FkQILRPSE2YgZkLA3CrqAmawzSJsgLt33zzzTFyHzQHHVTU5/bnqhgIFCkI0IwxtWCPh6EmBM0RRoqpyMwEg8S8w5sS2NgxC1GGfQHMLuQzIZgotAN03DezuI/AqoN8zEjEOaYezJPgHdxjzwABgImIcJiYhDA/EZgGpotQ4po2KMN+An3QBsKFPDa5YfKYiSibmIJ69eoVYyZjzyVgCCYgnoexEFCENhkrfWAmQ/iBi5nFV1q7d+8uNEE2hukP4cA546Ut6hBohPF4qrgIQCfQHWZNVgOYETGbYvqEfiruk/uTlRsEihhooYIA4sVUQ4QwtGOuU9sxM6E5E1Vs8ODBIsRe1WB+gUl27dpV5557ri655BJhxoEp8moljJYJk7RD+zBcGCn5bNjS36WXXireuoCBU5b9AYQOewSYXmj3+OOPFzZ6NHyEFUe0dbTznj17RtssDJ/yrEbatWsXI0dRlz4QMAg4+md8ZiaY+oUXXqhBgwZp3333jaYknpsEBl26dBH7GIyJ8TLxEXJc16lTRwjAI488Umwm82xshFMGpkAblPNUcRHgb86eEfRDBD3oz//uFffvXZGerFBBkDwkDK8oYuY+y2KOSR3Kc00+5+RzJI/z1EQe95I8zqlHfmpecs0xuc855ZMj5bkmJefcS87z1iM/uc859ShDSs1P7h1zzDHC1MPmcJJHHc5J1CFxjlbI666scLj2tGUgwN+/IPrZMp7en7K8IlCwIAjasTwpFYN1ktgn6feHw1WtenWl3st7TlnKHN+/v+rVry+u85bx67Bn4jRWJB39NhpxXB23gmnAMgK7Z84p/y/c2TBz7ZIlWj5julZ8M8NTHgyWzZyutd/P0spvZxaLDWWyv5+t5aGOY+m05DTgNLC5aWA5fH3Wd1qXnb0h0w9X+QTBiq+/0rynntC8kf/xVCAGT240LnNHbnxZx9vpzWnAaaBEaeCpJzX/lZeVvWJFYP0b/ssnCKo1bKh6Xbup6S49PZVdDPxv438bpwGngV9FAw132kl1d2yvzMqVN5QC4SqfIGjcqbN2O+8C9Tz7XE+OgdOA04DTQAWhgV7nDFSXY/urEnucgfmn/ssnCFJv+rkj4Ag4Ao7AZkagFLp3QVAKIHsXjoAj4AiUZQSKFgTr1mnFggVa8v3s9Wn5/PnKXrOm1J5p5U8/KXv16rT0ty4nRysWLlTO2rVpaS8n4LB0zpz12Cz9YY7W/rwRs3rpUq35+bygzhhLusZRUPue5wg4Ao7AxiJQpCDIyc7WxIcf1IQRt+nDB+7Vh/ffqw/u/Ze+ffedwEzXbGwfm1Ruyv+e1sIZMzapjaQyzJnnWfbjvCRrk45Lvv9eb/+/fwRM7tYHYHPfPZr67P+E8Prqzdc1+8MPCm1/8Xff6vuJHxV6329saQj48zoCmw+BIgVBWBDEd07bHNhXXY8/QV37n6CWe/bWvM+mRGbHsNeuXKmVixZpzfLlUqiwLmjd5JFWLV4srnOCBr5y8Yaa/dqVK7QipR5tUXZlqLN62bJYj7y1y5cpe81qrV6yRGtXrSIrJs7pd/XSJbEs78ZSj9XDqtBGdugzFgxjSsbCV5+rly0Nz5QT2lqp7NDeqjAu6lJm5U8LhbBYF+owZp6JfmiP69heyn/ZYUVQo0lTdTr6WHUL2HQOx+U//qiFX38dVgYrQx+5480JY1m1ZLFWhdVNThCutD83YDjr/fe0dnVumZRm/dQRcAQcgVJFoEhBwEgsM1M1ArOrvU0LkarVrStMIusCw1/0zUy9dcN1evPSQXrnn8O0OGjIy+bN0ydP/FsT7rg15F+kr15/VZ8HLXnM3y7Xhw/eF5nvgq+/0ribhumtywfrreuu1vxpXwrmODWUe+OSizTm2qs0d8qU9YJletCuxw79q96783atCox/yZzvNSGsUkZfdrFGDfmb5nzysRbPnqWx//i73rn5n3rjssH6PKwkcgLTXRZMWeNvGx7zJj/5f7HNtatWavJTT+ndW4frneE3ac7kSXrnluGivbeuu0o/Tv1MC2fO0Oi/X693gsY/6opLNO2Vl+IYwSRJjJkd+NrNt4nY1G29rWo2a6ZVy5ZIpvhbEwTklKdHavRfLwt4DNJn/x2pn779Rt+MHaN5743Xly88H8v5f46AI+AIbC4EihQE8DIY7/jbbtZrgRm+cvFF+ujB+1Vvu+1VrW69aPpo2K6dOp94ilS5sr4d/64UKi2eNUtNuvdQpxNO1ufPPaOcoGF3HHCS5sFgZ8zQopAadeysTiedpuqNGmtWMKHMCWaS7yaMV48zz9F2BxyoFQvmKycnR2tWLFdWlSrqcMKJWhLs8fOCgICR1ty6mTqfdoYadOykWe9PCOVWannQxpt06aoOxx6vH7+YGjXyaa+8qGoNGmmXgRcqIytLq4LtnkEumzdXtVu11s5/OUcr5v+oettvry6nnqFaLVtr1gfvK3vlKq2Y+4Oa99xNbQ7/o3749JOwkshW6s/MtCLsOcwNgoQ0ffQo/fj556odBEOAIfSXqYXTv9biwPi7nHK6drlgsBZM/yruKWy9886qF8a67b77pTbp546AI+AIlDoCRQqCdWE4WZWrqFXvvbTdvvurau3aqrftdmq5Z+/A5LJUp0VLrfxpkWZ/PFErFixczyhrBObepFMXNerUWTUCw26+a0816tBJtZo1V072WlWvXz+Un6/ZEz/U8sDw1wWGvyRstDbt1k2NA2Nv1Wfv0GcfZWRmKqtadTXbpacah/bqBMa9OpiNqtWpGxj/isCw34tMNSdnnWijWoOGom6DHdqp8s9lVgZzTPNddlX97duo41FHx2dQEExZVapom117qXoYS41GjbQqmJMQSMvm/RAF0Lp1OarRrJlahmdtGNrL2qpGMOOsVuoP3x3Lwri/fWu0ZoaVz7wgLFrvtbdqh9VT6EJmpqVhhVS/XXs1bL9jFDbNdt4lPPtCVapWXVVq1VaVGjVTm/RzR8ARcARKHYEiBQGjyQiafuPA0Fvv9Tt1P+lUrQq2+h8+/VhrgsljxhuvBebWVtvvt7/qbrutlGHiZ5kZsnAOc87IqqSMjEyyY8pevVrzggZdu0VLtT3oENVv0yYyzKwqVYJQWBAERXbsY9HMmYr1MzOVGcZAZY4IknlTJqt6vXpqs/+Batq1W2g/QwitzMqVFH9mQVBlysIYMitV1spFi2Jbi76ZEVYJKyUL/7Ky4n3e8vnhk09Uq0lTtTngIDXasWPMV/hlhLoyk2VkKDOUD1kb/MsJpqe6YXXUif2BsCrqeuJJarbTzsrIzFxfLqtKFa1cuEjZYT9iXZAOy+bOVUal3HHmBKG4vqCfOAKOQMVCoBw9TUbRY12nLAoEBsahRpMm2i4w/ZnjxgamPV8KDO/Ll1/QxIce0A/BvLMymEmCOh32ENYGWzw1FM7XaF3QrsN/yl69RjBWNnK/Chr0h/ffo5ljxmhV0MYb7NBeS4JJ6c2rh2jMsGuDmekdIQhy1q6NR4Uf55Hjm2lmsLF/9PCD0ca+JgindWHjNjukUEwK480JfZlMTcMqY+ozT2tU2HeYMvKpwJCDVh+kBmXXhXIWngHT1dej39RH4Tm+DuOiPe5nh01q2qMcY+Z8w7ROlSplqdJWW6lKzZqqHFYNCA3K5AQmvzYIvbotWwXz0Fd6a9j1Gn3NUP0w6dO4qkKQfff2OE0P/VLekyPgCDgCmwuBIgVBRkammgazTrV69dePr2Fg2C336B147Tr1OPlUtdx9z6gF9wj29Sadu6hS9a3UPJg/sqpVU1bQ5Fv16ROYZC1lBI26dTivHcxDbQ8+RK12763GwQzU69yBah5MN9WDiabXOeepaWijdZ99wmrh4Fhnm569VKNx49h/s2BXrx9WEK3D6mT7IJAatt1BPU49Xa323lvVGzbQdnvvE+swBuplVa2qpl26qctx/cWqpnM47hD6rhZWEy3Cc1WtXUeUaXtQX7Xus5cahPZ2OeMsbbPbHqrRqHFsz8zC+Gtqm169lFmpklJ/1QMuTbrvnC+fMk2D/b9+WC3UaNpU3U84SVuHlUvDDh2065lnB0GwrRqFPZJ2/Y4IbdeS/xwBR8AR2JwIZBTVuWVmCIYK40zKYZ7ZJjDROsEOXiXYuNv//g9q2/cQbd1jpygAqtSqpaaB6VUKgiAjMM6WvXYPzK6mLCNDLcJ59QYNVL1+A7UNDJm6jXbsEMtnVami6g0aasewMbtd2EBFw1b4NQvtbhXyw2lgpt1VN+wT0Md2Yc+iwx+PEsKnadfuqtmkqVoFe35GZqbom3ocM7KyhBDYsd/hqhfqtghMvlrdutq6e484LoVftbr11ObAvupwxJGxbNPAxGuxP7D7HjKzqOk377FzPoZfLQiUZkE40Yfy/HiuuqE/M1Ot5s3VPvTf4fAj4zgVfllVqqjdoYfFcYRL/1duEfCBOwLlH4GM8v8I/gSOgCPgCDgCm4KAC4JNQc/rOgKOgCNQARDIJwiWzpmjb98eq2/fedvTLxg4Fo6F04DTQDmngXGa8/FEZScv1aQIsHyCYO6USXr/rhF6d8RtnhwDpwGnAaeBCkIDE0bcrklP/p/WLF+WIgJyT/MJgmotWqrxwYeq0QF9PTkGTgNOA1seDVTQv3njvoeoQe8+yqhSNZf7p/yfTxBUrltPNdp3UI127T05Bk4DTgNOAxWFBgJf32rb7VXQW475BAFCwvjPkyPgCDgCjkDFQYAPaAt5mgIFQWrZzMxMZWVlbZDMLLVIqZ0zFrP8fWdkZMT3/TkyVo7JoDg3+6UO18k9P27JCJTMs0Nf0CC0mvRgZuKafBJlVMAvKZN638zW1zX7hY7lP0cgjQhkFNVWTk6ORo0apX//+996/PHHY3ryySf1/fffR8ZbVN1032MsY8aM0ezZszfoOzs7W19//bVWrlyp6dOn67///a+mTZsWu2diTZkyRT/88EOsg6uIzz//PJY180kVQfL/0oaAmUXae+KJJwStQm8w9eXLl+ull17SY489FufSu+++G7/MTzo2M0Hfr776qv7zn/9EOjbLpc8VK1bEOcj8++4j0y0NAAAQAElEQVS77yIdJ/X86AikC4FCBYGZae3atYKoIU4I+cUXX9Rrr72mH3/8sdQJkrE88sgj+uKLL6KGBABMMoTA+PHj45huu+02VapUSc8//3xk/j/99JOeffZZISwojzZG/Y8++miDicg9T47ApiAALX755Zf629/+ppEjR+qOO+7Q/fffH+kM5eVf//qXmD8vvPCCPvnkk5if9IcQ4P6IESNi3RtuuCHSM/lPPfWU7rzzTj3zzDO66qqrNhASSX0/OgKbikChgoCGzUxbbbWVrrvuuqjNPProo7rnnnvUuXNnrVmzJjLbGTNmaN68eVFocESDMbOodaOJoxUtXbpU3377bUzcp+3Fixdr4cKFQstBmyf/m2++2aCMmWnZsmUxj7LUS01mJrSr7bffXjB9NK/evXuratWqcUwvvfRSHGvz5s3jxGMs7du312effaYlS5akNuXnjsAmIYCSAZPfc8899dBDDwmmjpLC/Jg7d646duy4fg795S9/EYKDDjmywmYeoMggQBo1aqSvvvpKCxYsEMLliiuuiKtx5h0rjUSxob4nRyAdCBQpCOgATXzy5Ml67733NGHChKiRIARef/113Xjjjfr73/8ej59++qkQFCxvMcmMHTs2Ei/C4fbbb9f1118fBQqaz6JFi6KGg+bDhKHuAw88oGHDhsW2WIXAqGH+N998s6655hrdeuutUfAwcRgXxzlz5sSJsu2226phw4aqVauWxo0bF4XU/Pnz41j32GOPuCIwy11+Uw7hwsQzy11+054nR2BTEUCDr1u3bmTyCAbmDsoQCZqDlu+66y5NnTp1fVfUadKkia688spIt9xHSYKmOdasWVOJItOjRw+Rx/xb34CfOAJpQCCjuDbQsrFPwohvueWWaPs0s6h177LLLoI4YchoMDDdSZMmRe0c23yvXr304YcfCk180KBBuuSSS+KS9/33348aOUz5ggsuiHkw5mOOOUZHHXVU3INAE3rllVfiyuLSSy/VYYcdFttJxosgYAVhZnHVQlt//vOfo9mIVQHj2XXXXfXWW29FTWxGWLmY5Y4bgYGAMnNBkODpx01DAC2d+fDGG29oyJAhGj58eFzJstolVa9ePSormFXZc1u1apWSH7TMKhbhweqWVThzBCHB3DGzSPuYPemH/KSuH1MQ8NPfjECxggCNZPDgwUKbQRgcfvjhUcOG0T/99NOaOXOmatSoEfO6desWB4K5BoJlOQyDZ0m73XbbqU2bNtp5550jo4f4O3TooMaNG0ct55133on2VTSjl19+ObaLgKEukwOhwjF1EjDBKleuHJk/HWP22WeffaKQYjLRx8cffyw0LiZoMqmYUKtXr6aKJ0cgLQhA7z179tTJJ5+sFi1a6KCDDlK7du1UpUqVqNywd3DkkUdqwIABgvZYFZv9oohAm1tvvbWOOOII7bvvvho1alRcWUDD3DOzaH5FWJCXlkF7I47AzwgUKwgoB7OtVq2aSJyj1cyaNUuXXXaZBg4cKJaxTISqwTYP8T/33HNq1qyZateurQYNGkSbPHWwlbJCqF+//nrmTfto6AcffLBYFrMHgTBAYKDl85YPdTFPoeWb/TJ5GAvL5FThgKaFqWnHHXeMKxP6atq0aTynHJOKsSIM6NuTI5AOBGDQmEtRkI477rjI7KFbaA/TKC9bsLpGcYJuWSEktAtdn3vuufGNI/YFoHVMTMwfTErMAfbAeCmCfOrLf45AGhEoVhCgWWPzT/qEkbJKgElj72ePAOKHuWITZRUAgbdu3TquElguY9fkjYehQ4fG7xEwJ6EpMXnQbrp06RKXvggC9gwgeNrr27dvnFDUYzmNoEkYOEwdzYu+2Esws6hBsfHG5KF/hBIT6IMPPhCrCZ4DQUFC+6IN+a+cIVA2hwu97rDDDtH+f/HFF+u+++6LqwJolDnAKhfFiY1k6B0m//bbb8e9N8qw8v3nP/8ZV8UoWccee6xg+pTlbTnqsu+F+ZU5UzZR8FGVVwQKFQQwfBg1BMlmVSrTxBR0/PHHq3///jr66KPFWw2YZKjTsmVLnX/++UIjpw4a0Zlnnim0JMqfffbZ0RxE+e7du8flLkz5pJNOikvoP/7xj3H5zOSoU6eO0JTo64QTToiTBPMPky5pG4bPfgJ/APpn8uy3335x3wBzFEttTFZ9+vSJr7yyN4BAwCRFHU+OQDoQgPag2XPOOUfMmQsvvFDQoZlFcyhzAtMQbwztv//+sUtMpa1atRIaPnPj9NNPj/PkvPPO0zbbbBMVG8qedtppcZ4xd8inr9iA/+cIpAmBQgUB7ZtZ1KRZFXCdmurVqyc0HbR7GC7MnPto+lwndcwsMv6kLMydcpTHbAND5zppjw1emLRZ7gYZZXbaaadofkLjon7qROB1PZbbrALIx8yU1KddBAV7Eaxi0KQwGzEBKcd9T45AOhGArqHhtm3bRvMnNAndwfB32223qCAxR6B7lCRolTKsdFkVME/IS8bEKhj6ZY+MVXiS70dHIJ0IFCkIiusIAk7SxpYtqlxKWxsUI3+DjJ8vmEwwdTbpfs4q8oAZCWGCYDH7Za+hyEp+0xH4lQhAr6S81cgj5c1PrrlHSq6TI3mk5NqPjkC6EShQEJhZXJaiyZT1hPkK7QtzVXFjxSSEdsVGnVn5ecbinsvvZ5QbevW/lf+tNicNmBWsAOcTBGa5BWGw5SUB7MaO9deU3dg2vdyGTgkdD8fjV9FAHqeWXrdk6AfeZ5bL35Xnl08QYIPnbRs2ZT21l2PgGDgNOA1UBBqAr/MyD4I2jxxQPkGA1KCgp5KRyo6r4+o04DSwuWgA83heIcB1PkFApidHIP0IeIuOgCNQVhFwQVBW/zI+LkfAEXAESgkBFwSlBLR34wg4Ao5AWUVgowQBbnRx8cAxeZAZM2aIoBnEGsCPCn5VJCW303bk/Wkcx+GEiy+Kk4bJxy02XxUT64BP+nGDnXxYNnHixOiSOimPV1R8uiTXfnQESgoBnMoRzAnXK6l94O4Ex41DhgwRblNwu0LUMuZQajk/dwRKG4FiBQEfbeH756abborRypIBEnQD4QAR42QOh3LJvXQeYfj4CiIyGr6MkrZh/g8//HC8JHoTk4+vhhEG+B4iShlfcMYC4T88ld59993C8Ve49H+OQIkggLKCXyFco6TGHaAz7uEvCIUKVyeEVcXfUCpdU86TI1DaCBQrCIgchjb9u9/9LoaARKthkLxdxGfxZhbDQ5qZcOYGU7722mvjaoFrooEhSBAoED0eGmHunDNRYMzEQb766qs1evTo6HyO9hEseG0kahPn7LKTT6It2uGTfD67x111p06dxIdltIlA4PN9HHZRnsR9BATuqLn25AiUBALQHy7V+eIdOk3tAzcqeNYlChmCAnrFbTUO6FLLlZtzH2iFQaBYQYDJh5gCOH7DORaadkFPb2Zi1UDwGaKZsezFOymC43//+18MRMM1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Among them, leucyl-tRNA synthetase 1 (LARS1) plays a central role in sensing leucine and activating mTORC1, thereby linking nutrient availability to metabolic regulation. Despite recent progress, fundamental questions remain unresolved about the regulatory mechanisms governing LARS1\u0026rsquo;s non-canonical functions, particularly its structural organization within the MSC. Here, we employed an integrated approach combining structural, biochemical, and cellular analyses to investigate the noncanonical roles of LARS1. The cryo-EM structure of the LARS1:Isoleucyl-tRNA synthetase 1 (IARS1) complex revealed that LARS1 binds to IARS1, its anchoring partner in the MSC, via its UNE-L domain. Amino acid stimulation induces LARS1 phosphorylation at Ser1070, Ser1077, and Ser1082, which are located at the interface with IARS1. These modifications disrupt the interaction, promote LARS1 dissociation from the MSC, and enable mTORC1 activation. This study highlights phosphorylation as a conserved and critical molecular switch that orchestrates the non-canonical functions of MSC by dynamically modulating the assembly and activity of its components in response to external stimuli.\u003c/p\u003e","manuscriptTitle":"Cryo-EM Structure of the LARS1-IARS1 Complex Reveals A Nutrient-Responsive Switch Controlling mTORC1 Signaling","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-09-01 10:05:49","doi":"10.21203/rs.3.rs-7316280/v1","editorialEvents":[],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"nature-communications","isNatureJournal":true,"hasQc":false,"allowDirectSubmit":false,"externalIdentity":"NCOMMS","sideBox":"Learn more about [Nature Communications](http://www.nature.com/ncomms/)","snPcode":"","submissionUrl":"https://mts-ncomms.nature.com/","title":"Nature Communications","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"ejp","reportingPortfolio":"Nature Communications","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"c3e0a47a-c887-4c2c-b2f7-c2fd4754db86","owner":[],"postedDate":"September 1st, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[{"id":53996687,"name":"Biological sciences/Biochemistry/Structural biology/Electron microscopy/Cryoelectron microscopy"},{"id":53996688,"name":"Biological sciences/Cell biology/Cell signalling/Nutrient signalling"},{"id":53996689,"name":"Biological sciences/Biochemistry/Proteins/Intracellular signalling peptides and proteins"}],"tags":[],"updatedAt":"2026-04-18T13:50:15+00:00","versionOfRecord":[],"versionCreatedAt":"2025-09-01 10:05:49","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7316280","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7316280","identity":"rs-7316280","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}