Nanoparticles Based Antimicrobial Susceptibility and Molecular Characterization of Extended Spectrum Beta-lactamase (ESBL) Producing Pathogens

Abstract Antibiotic resistance is a global challenge that’s correlated with the increasing illness and mortality rates, so advanced antibacterial agents should be produced. Most of the urinary tract infections are due to the antibiotic resistance. Microbes develop defense because of misuse & excessive use of drugs. Those microbes produce extended spectrum beta lactamases (ESBL), plasmid encoded hydrolytic enzymes. They confer resistance to β antibiotics.Nanotechnology brought huge advancement for the UTIs treatment based upon the usage of metal nanoparticles such as silver NPs. Nanoparticles are the nano-scaled materials(under 100nm). Nanoparticles consists of wide range of antibacterial characteristics against the negative & positive bacteria, and could resolve the issue of microbial drug resistance. NPs can be used as an alternative to antibiotics to treat UTI. Herein, we utilized Moringa oleifera leaves extract to synthesise the silver nanoparticles (Ag-NPs) biogenically. The characterization of Ag-NPs was executed by UV-spectroscopy, scanning electron microscopy (SEM), FTIR, and X-ray diffraction XRD.The antibacterial activity studied against the bacterial isolates & UTI strains. We used the well diffusion method to evaluate the antibacterial activity Ag-NPs, we utilized 1ml of DMSO in which 40ml of Ag-NPs were added Serial dilutions were 1000µl, 500µl, 250µl and 125µl. The bacteria that were most responsive to it were gram positive Staphylococcus aeruginosa and to some extent gram negative Escherichia coli bacteria. The zones of inhibition were measured by employing the common ruler.