Mechanism of Regulating Ectopic Endometrium Protein Expression by Bushen Huoxue Recipe in Treatment of Endometriosis Associated Infertility

Objective: To compare the uterus protein expression differences between endometriosis( Ems) correlation infertile patients and normal women of reproductive age,and study the mechanism of Bushen Huoxue recipe( BSHXR). Method: Four cases of Ems infertility patients with kidney deficiency and blood stasis syndrome diagnosed by laparoscopic surgery wereobserved. Ectopic endometria were collected during the laparoscopic surgery as Ems group. BSHXR was given to Ems patients after the surgery,lasting for three months,the ectopic endometria were collected after the treatment course as BSHXR group. Besides,four healthy women's endometria were collected three months after drug-induced abortion as normal control group. All the endometrial samples were pathologically diagnosed as secretory phase endometrium. Differential centrifugation and density gradient centrifugation method were applied to extract purified endometrial proteins. Difference gel electrophoresis( DIGE) technology were used to get the expression patterns of ectopic endometria differential proteome. Decyder difference 2-D analysis software were used to analysis. Matrix-assisted laser desorption ionization mass spectrometry( MALDI-TOF/TOF) were used to identify among these differential protein spots. Result: Through establishing the differential proteome technology platform of ectopic endometrial proteins among 3 groups. 35 different express proteins were found by differential software analysis, 28 different proteins were identified by using mass spectrometry identification. Conclusion: Five possible mechanisms of Ems-associated infertility were predicted involving 10 different express proteins. BSHXR can regulate 26 different express proteins by 4 possible mechanisms to treat ectopic endometria of patients with Ems-associated infertility.