Reduction of Body Fat Mass by Cryolipolysis without Changes in Clinical Biomarkers Levels

Cryolipolysis is a non-invasive cooling technique used to reduce localized body fat mass. The changes in fat percentage composition have been assessed by many analytical methods, including bioelectrical impedance analysis. The most common effects of cryolipolysis on reducing subcutaneous of visceral fat tissue have been addressed but biochemical parameters to safely evaluated its systemic effects have been not fully understood. This study aims to systematically analyze potential changes in hematological and inflammatory biomarkers following the reduction of body fat mass. A prospective longitudinal approach included a total of 20 participants divided into groups of 5 men and women in each with ages between 25 and 45 years. They were submitted to standard cooling exposure for 60 minutes at -5°C and -10°C with 360° and “shielded” equipment, respectively. The blood samples were analyzed at times T0 (baseline), T1 (2 days), T2 (14 days), T3 (30 days) and T4 (60 days) after the procedure. The percentage of body fat mass were analyzed by bioimpedance at times T0 (baseline), T1(30 days), T2 (60 days), T3 (90 days) and T4 (120 days). No changes in hematological and inflammatory biomarkers serum levels were observed in both temperatures. However, bioimpedance analysis revealed a significant reduction in the percentage of body fat mass only in men participants, with a decrease of 28 (24.5/ 29.9) compared to 30 (26.2/ 33.30) when submitted to -5°C after 120 days. These findings support cryolipolysis can be considered an effective and safe method for localized body fat reduction without significant changes in blood biochemical parameters.