The Radioprotective and Radiomitigative Effects of Resveratrol Supplementation at Different Time After the Start of Irradiation on the Sperm Count and Quality of Male Mice

Background: Agents with free radical-scavenging features may act as radiation modifiers, protectors, or mitigators. Methods: We investigated if supplementation of resveratrol (RSV) in mice, since different time after the start of irradiation may influence the sperm count and quality during the irradiation and recovery. Results: Irradiation significantly reduced the sperm count. RSV supplemented with 1 Gy since 24 h increased sperm count. Combination of low doses increased, whereas of high doses reduced DNA damage. Coadministration of two high doses since 8th day significantly increased of DNA damage and slightly sperm count. The supplementation of RSV during recovery was toxic to irradiated males. The sperm parameters were a little better in the absence of RSV. The level of DNA damage of germ cells was significantly lower in groups combined with 1 Gy. Conclusions: Resveratrol counteracted the killing of gem cells by ionizing radiation and is very useful to improve the sperm count. RSV may work both as radioprotector and radiomitigator of lethal effects in male gametes. Combination of high doses of irradiation with RSV since 24 h mitigated of DNA damage. Contrarily, the supplementation during the recovery is not recommended since it may works toxic during long-lasting irradiation.