In-vitro study of microtrauma following insertion of self-tapping or self-drilling mini-implants into a postmortem human mandible 

In-vitro study of microtrauma following insertion of self-tapping or self-drilling mini-implants into a postmortem human mandible | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article In-vitro study of microtrauma following insertion of self-tapping or self-drilling mini-implants into a postmortem human mandible Mona Sallam, Robert Ciesielksi, Helge Fischer-Brandies, Bernd Koos This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8896603/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Introduction: The study investigated the formation of microcracks surrounding self-tapping and self-drilling mini-implants, depending on whether pilot drilling was performed beforehand. A postmortem human mandible used for measurement of microstructural damage. The null hypothesis proposed that the method of mini-implant insertion would not affect the formation or progression of microcracks in the cortical bone of the mandible. The alternative hypothesis assumes that self-drilling screws cause greater cortical damage than self-tapping screws. Methods: On a mandible with soft tissues removed, a 50-square reference grid was applied to the bone surface. Subsequently, 20 self-tapping and 20 self-drilling miniscrews (tomas®-pin, tomas®-pin SD; Dentaurum, Ispringen, Germany) were inserted. Additionally, pilot drillings without implant placement, were created and served as control drillings. The grid was then cut into 50 bone blocks, followed by horizontal separation of 100-μm-thick cortical discs to be evaluated for periimplant microcracks and structural irregularities by light and electron microscopy. The effects of the three groups were analyzed using seperate one-way analyses of variance (ANOVA) for each variable. In addition, the non-parametric Kruskal–Wallis test was applied, due to the violations of the normality assumption. To maintain a significance level of 5%, pairwise post hoc comparisons were performed using the Bonferroni correction. Results : A similar number of microcracks, as well as comparable crack length, accumulated crack length, and crack width, were observed around both self-drilling and self-tapping Temporary Anchorage Devices (TADs). Minimal microcrack formation was observed around pilot preparations without TAD insertion. Conclusion: No significant differences in microcrack formation were observed between self-drilling and self-tapping insertion techniques. Future clinical studies may explore regional differences in microcrack distribution within the jaw. human mandible orthodontic mini-implants self-tapping self-drilling microcracks Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8896603","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":611316845,"identity":"5521afed-cf99-418f-ac5b-27df04a983d2","order_by":0,"name":"Mona Sallam","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA5ElEQVRIiWNgGAWjYBADGT4GBsYHDAwHQBwDorTwsDEwMBuQrIVNgigtuu29Bz8w/LHjYWM/nVbNU3NHjp+BeeMDfFrMzpxLlmBsS+Zh48nddpvn2DNjyQa2YrzWmN3IMZBgbGAGOgykhe1w4oYDPGYSBLQY/2D4U8/Dxv92WzHPP7AW8x8EtADNZDvMwyaRu42Ztw1iCz4dQL+cMbNIbDsO1PJ2s+TcvsPGks1sxfgddrzH+MaHP9Vy/Py5Gz+8+XZYjp+9eeMHvNaAQAKUZuIBkcwE1SMBRrzeHgWjYBSMghELAMnGRnxgU/lXAAAAAElFTkSuQmCC","orcid":"","institution":"University Hospital Schleswig-Holstein","correspondingAuthor":true,"prefix":"","firstName":"Mona","middleName":"","lastName":"Sallam","suffix":""},{"id":611316846,"identity":"7c107ec5-2214-4513-9f22-a5f5cd45599d","order_by":1,"name":"Robert Ciesielksi","email":"","orcid":"","institution":"University Hospital Schleswig-Holstein","correspondingAuthor":false,"prefix":"","firstName":"Robert","middleName":"","lastName":"Ciesielksi","suffix":""},{"id":611316847,"identity":"bbe993a6-8f66-40a3-8626-3224138022e6","order_by":2,"name":"Helge Fischer-Brandies","email":"","orcid":"","institution":"University Hospital Schleswig-Holstein","correspondingAuthor":false,"prefix":"","firstName":"Helge","middleName":"","lastName":"Fischer-Brandies","suffix":""},{"id":611316848,"identity":"e96cfc10-c5b0-4bb2-b0c3-36ed3d204a76","order_by":3,"name":"Bernd Koos","email":"","orcid":"","institution":"University Hospital Schleswig-Holstein","correspondingAuthor":false,"prefix":"","firstName":"Bernd","middleName":"","lastName":"Koos","suffix":""}],"badges":[],"createdAt":"2026-02-16 23:08:23","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-8896603/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-8896603/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":106729604,"identity":"95ec89cc-1acd-40cd-be28-a92f6b298533","added_by":"auto","created_at":"2026-04-12 19:24:16","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":794880,"visible":true,"origin":"","legend":"","description":"","filename":"ArticleclinicaloralinvestigationsV2.pdf","url":"https://assets-eu.researchsquare.com/files/rs-8896603/v1_covered_6bcee854-f664-42ce-a6dd-104d1397fd5c.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"In-vitro study of microtrauma following insertion of self-tapping or self-drilling mini-implants into a postmortem human mandible ","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"human mandible, orthodontic mini-implants, self-tapping, self-drilling, microcracks","lastPublishedDoi":"10.21203/rs.3.rs-8896603/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8896603/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cstrong\u003eIntroduction: \u003c/strong\u003eThe study investigated the formation of microcracks surrounding self-tapping and self-drilling mini-implants, depending on whether pilot drilling was performed beforehand. A postmortem human mandible used for measurement of microstructural damage. The null hypothesis proposed that the method of mini-implant insertion would not affect the formation or progression of microcracks in the cortical bone of the mandible. The alternative hypothesis assumes that self-drilling screws cause greater cortical damage than self-tapping screws.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethods:\u003c/strong\u003e On a mandible with soft tissues removed, a 50-square reference grid was applied to the bone surface. Subsequently, 20 self-tapping and 20 self-drilling miniscrews (tomas®-pin, tomas®-pin SD; Dentaurum, Ispringen, Germany) were inserted. Additionally, pilot drillings without implant placement, were created and served as control drillings. The grid was then cut into 50 bone blocks, followed by horizontal separation of 100-μm-thick cortical discs to be evaluated for periimplant microcracks and structural irregularities by light and electron microscopy. The effects of the three groups were analyzed using seperate one-way analyses of variance (ANOVA) for each variable. In addition, the non-parametric Kruskal–Wallis test was applied, due to the violations of the normality assumption. To maintain a significance level of 5%, pairwise post hoc comparisons were performed using the Bonferroni correction.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults\u003c/strong\u003e: A similar number of microcracks, as well as comparable crack length, accumulated crack length, and crack width, were observed around both self-drilling and self-tapping Temporary Anchorage Devices (TADs). Minimal microcrack formation was observed around pilot preparations without TAD insertion.\u003cbr\u003e\n \u003cstrong\u003eConclusion:\u003c/strong\u003e No significant differences in microcrack formation were observed between self-drilling and self-tapping insertion techniques. Future clinical studies may explore regional differences in microcrack distribution within the jaw.\u003c/p\u003e","manuscriptTitle":"In-vitro study of microtrauma following insertion of self-tapping or self-drilling mini-implants into a postmortem human mandible ","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-03-25 17:28:30","doi":"10.21203/rs.3.rs-8896603/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"e3d012c4-0053-4819-84c0-1c0c021da421","owner":[],"postedDate":"March 25th, 2026","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2026-04-12T19:23:54+00:00","versionOfRecord":[],"versionCreatedAt":"2026-03-25 17:28:30","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8896603","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8896603","identity":"rs-8896603","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}