The Impact of Eosinophil Cationic Protein on Nasal Epithelial Cells: An Investigation of Cellular Responses

Abstract Introduction The impact of eosinophil cationic protein (ECP) on nasal epithelial cells remains unclear. Our study investigated whether ECP could activate nasal epithelial cells to secrete interleukin 8 (IL-8). Materials and Methods The airway epithelial cells, NCI-H292, were cultured separately and exposed to varying concentrations of ECP for 2, 4, 6, 16, or 24 hours. The supernatant was collected and IL-8 was tected using ELISA. Results The results showed a significant increase in IL-8 levels in the ECP group compared to the control group at 24 hours (50.92ug/ml±20.06% versus 1.95ug/ml). Conclusion The study concludes that ECP stimulates H292 cells to secrete high levels of IL-8, causing damage to epithelial cells. The increase in ECP in allergic disease can aggravate inflammation of the airway mucosa.