Relationships of expression of KGF and MVD with endometriosis

Objective To investigate expression of keratinocyte growth factor(KGF) and change in microvessel density(MVD) in eutopic and ectopic endometria of women with endometriosis(EMs) and their correlation with clinical staging and menstrual cycle of the women.Methods The expressions of KGF and CD4 were detected by using immunohistochemical SP methods.MVD was calculated according to expression of CD34 in the vessels.Results The positive expression rate of KGF in normal endometria,eutopic and ectopic endometia in endometriosis was increased orderly(H=31.02,all P0.01).The expression of KFG both in normal endometria and eutopic endometria in endometriosis was affected by menstrual cycle of the women: that in secretory phase was 92.3% and 85.71% in proliferative phase respectively(in normal endometrium group: P=0.001;in chocolate cyst of ovary group: P=0.017).The expression of KGF was not associated with clinical stage of endometriosis(P0.05).Also,MVD in normal endometria,eutopic and ectopic endometia in endometriosis showed an orderly increased tendency(H=79.13,all P0.01),and that in secretory phase of either normal endometria or eutopic endometria of endometriosis was higher than that in proliferative phase(in normal endometrium group: t=5.28;in eutopic endometria of endometriosis group: t=6.42,both P0.01).Although angiogenesis was seen in every phases of menstrual cycle,no associations between angiogenesis and clinical stages were found(P0.05).In endometriosis,the expression of KGF and MVD were closely correlated with each other(r=0.73).Conclusion The expression of KGF and increased microvessel angiogenesis are associated with development of endometriosis and also the expression of KGF and MVD are closely correlated with each other in occurrence and development of endometriosis,which may provide new targets for treatment of endometriosis.