Effects of N-Carbamylglutamate on Lactation Performance, Milk Metabolome, and Fecal Microbiota in Liaoning Cashmere Goats

Effects of N-Carbamylglutamate on Lactation Performance, Milk Metabolome, and Fecal Microbiota in Liaoning Cashmere Goats | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Effects of N-Carbamylglutamate on Lactation Performance, Milk Metabolome, and Fecal Microbiota in Liaoning Cashmere Goats Wei Ma, Shuai Chang, Jianwei Wei, Di Han, Chunyan Wang, Zhiguo Quan, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9356585/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background This study evaluated the effects of dietary supplementation with N-carbamylglutamate (NCG) on lactation performance and intestinal microbiota in Liaoning cashmere goats. Methods Sixty pregnant does were randomly allocated to a control group (basal diet) or an NCG group (basal diet + 0.09% NCG) for 75 days. Results The results showed that NCG significantly increased colostrum lactose, casein, and IGF-1 (P < 0.01), as well as casein and IgA in mature milk (P < 0.05). Lambs from the NCG group had higher body weights at 10 and 45 days of age (P < 0.05) and a 57.05% reduction in diarrhea incidence. Metabolomic analysis identified differentially abundant metabolites in milk, which were notably enriched in pathways such as folate biosynthesis. Fecal microbial profiling revealed that NCG supplementation increased the relative abundance of Spirochaetota and decreased that of Arcanobacterium. Conclusion In conclusion, NCG improves lactation performance and offspring health in cashmere goats, likely through modulation of milk metabolite profiles and gut microbiota structure. N-carbamylglutamate Liaoning cashmere goat lactation performance milk metabolome fecal microbiota Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9356585","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":634301547,"identity":"77603c1e-07db-47a4-82f7-0bf4b07b45f2","order_by":0,"name":"Wei Ma","email":"","orcid":"","institution":"Jinzhou Medicial University","correspondingAuthor":false,"prefix":"","firstName":"Wei","middleName":"","lastName":"Ma","suffix":""},{"id":634301550,"identity":"26842ecf-d5ee-4b4a-9ade-2e0ee1ebee89","order_by":1,"name":"Shuai Chang","email":"","orcid":"","institution":"Jinzhou Medicial 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Liaoning Cashmere Goats","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"N-carbamylglutamate, Liaoning cashmere goat, lactation performance, milk metabolome, fecal microbiota","lastPublishedDoi":"10.21203/rs.3.rs-9356585/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-9356585/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eBackground\u003c/h2\u003e \u003cp\u003eThis study evaluated the effects of dietary supplementation with N-carbamylglutamate (NCG) on lactation performance and intestinal microbiota in Liaoning cashmere goats.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e \u003cp\u003eSixty pregnant does were randomly allocated to a control group (basal diet) or an NCG group (basal diet\u0026thinsp;+\u0026thinsp;0.09% NCG) for 75 days.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eThe results showed that NCG significantly increased colostrum lactose, casein, and IGF-1 (P\u0026thinsp;\u0026lt;\u0026thinsp;0.01), as well as casein and IgA in mature milk (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05). Lambs from the NCG group had higher body weights at 10 and 45 days of age (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05) and a 57.05% reduction in diarrhea incidence. Metabolomic analysis identified differentially abundant metabolites in milk, which were notably enriched in pathways such as folate biosynthesis. Fecal microbial profiling revealed that NCG supplementation increased the relative abundance of Spirochaetota and decreased that of Arcanobacterium.\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e \u003cp\u003eIn conclusion, NCG improves lactation performance and offspring health in cashmere goats, likely through modulation of milk metabolite profiles and gut microbiota structure.\u003c/p\u003e","manuscriptTitle":"Effects of N-Carbamylglutamate on Lactation Performance, Milk Metabolome, and Fecal Microbiota in Liaoning Cashmere Goats","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-05-07 09:48:41","doi":"10.21203/rs.3.rs-9356585/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"e574628c-71c7-4b21-8d06-76cb5aa170f4","owner":[],"postedDate":"May 7th, 2026","published":true,"recentEditorialEvents":[{"type":"decision","content":"Rejected","date":"2026-05-12T11:25:51+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-05-11T18:43:56+00:00","index":45,"fulltext":""},{"type":"editorInvitedReview","content":"","date":"2026-05-09T10:58:38+00:00","index":41,"fulltext":""},{"type":"reviewerAgreed","content":"328596235614711430674433456470987627566","date":"2026-04-29T17:24:47+00:00","index":38,"fulltext":""},{"type":"reviewerAgreed","content":"312481608349580028499574648596234656147","date":"2026-04-29T17:20:53+00:00","index":37,"fulltext":""},{"type":"reviewersInvited","content":"9","date":"2026-04-29T16:17:11+00:00","index":"","fulltext":""}],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2026-05-12T13:20:11+00:00","versionOfRecord":[],"versionCreatedAt":"2026-05-07 09:48:41","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-9356585","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-9356585","identity":"rs-9356585","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}