WITHDRAWN: Endurance Training Alleviates Metabolic-associated Fatty-liver Disease (MAFLD)-related Testicular Impairments via ER Stress Regulation

doi:10.21203/rs.3.rs-6234592/v1

WITHDRAWN: Endurance Training Alleviates Metabolic-associated Fatty-liver Disease (MAFLD)-related Testicular Impairments via ER Stress Regulation

2025 · doi:10.21203/rs.3.rs-6234592/v1

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Abstract

Abstract Metabolic-associated fatty liver disease (MAFLD), the most prevalent liver disorder globally, affects 20–40% of the population and presents significant health challenges. Studies link MAFLD to male reproductive dysfunction, highlighting the need for effective interventions. This study investigates the impact of MAFLD on testicular function and evaluates the protective role of endurance training, with a focus on the PERK-EIF2α-ATF4 signaling pathway. Forty-four rats were allocated into two dietary groups (n = 22 each): a standard diet control group (C) and a high-fat diet supplemented with fructose water group (FL). After 17 weeks, histological analysis confirmed MAFLD development in the FL group, while the control group showed no pathological changes. Each dietary group was further subdivided into sedentary and endurance-trained (T) subgroups (n = 10 per subgroup), resulting in four experimental groups: C, C + T, FL + T, and FL. At the end of research, thyroid hormones (T3, T4, TSH), sex hormones (testosterone, LH, FSH) as well as PERK, EIF2α and AFT4 expression were assessed. Our result indicated that MAFLD led to significant weight gain, disrupted serum levels of thyroid hormones, and impaired sex hormone profiles. Additionally, MAFLD triggered ER stress, evidenced by dysregulated expression of genes in the PERK-EIF2α-ATF4 pathway. Remarkably, endurance training mitigated these adverse effects by normalizing hormonal profiles and restoring the expression of ER stress-related genes. These findings highlight the critical role of ER stress in MAFLD-induced male reproductive dysfunction.

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Research Square has withdrawn this preprint due to an unresolved authorship dispute. The manuscript was posted without consent from all listed authors, which constitutes a breach of Research Square’s Terms of Service. Editorial notes are used to provide important context regarding the topic of a preprint or to alert readers to potential issues concerning that preprint or a downstream publication associated with it. For more information on editorial notes, see our Editorial Policies . Abstract November 21, 2025. Research Square has withdrawn this preprint due to an unresolved authorship dispute. The manuscript was posted without consent from all listed authors, which constitutes a breach of Research Square’s Terms of Service. Biological sciences/Cell biology Biological sciences/Physiology Health sciences/Endocrinology Full Text November 21, 2025. Research Square has withdrawn this preprint due to an unresolved authorship dispute. The manuscript was posted without consent from all listed authors, which constitutes a breach of Research Square’s Terms of Service. Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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