Synergism between Tocotrienol and i-PRF in HFSCs proliferation: an in-vitro study

Introduction: Androgenic Alopecia (AGA) is an early onset of hair loss found in both males and females. With an increase in stress and hormonal imbalances, the need for a better and faster alternative to today’s medicine for alopecia is increasing. Adding an adjunct like Aloe Vera to the gold standard platelet concentrate in the treatment of alopecia may boost hair growth. To find an optimum concentration and evaluate the synergism between Aloe Vera and injectable platelet-rich fibrin (i-PRF) is the aim of this in-vitro study. Materials: & Method: Hair follicles from an individual suffering from AGA were prepared and stored in an incubator. Stem cell characterization was done and stem cells were multiplied. Hair follicle-derived stem cells (HFSCs) were subjected to different concentrations of Aloe Vera, i-PRF, and i-PRF with Aloe Vera in different Petri dishes. Result: Aloe Vera group shows an increase in HFSCs proliferation up to a certain concentration, after that it shows cell senescence. I-PRF on other hand shows a direct relation between concentration and cell proliferation. The combination group at a particular concentration shows double the cell proliferation than the rest of all. Conclusion: Alopecia and its treatment are in a wide range and have evolved with time. No single treatment modality can be sufficient to treat a disease at its best. Adjunct therapy when incorporated into the standard treatment results in faster and better hair growth. Also, the optimization of the concentration of any product is of great value.