The Effect of Alarelin on the Sex Steroid Receptors of Entopic and Ectopia Endometrium in Experimental Endometriosis in Rats

Objective To study the effect of alarelin on experimental endometriosis in rats, measure the changes of the sex steroid receptors in entopic and ectopic endometrium for exploring the mechanism of the effect of alarelin on endometriosis. Methods 27 rat models of endometriosis were divided into 2 groups (at random), model group and non-treatment group, alarelin group with alarelin 30 μg (kg·d) -1 injected subcutaneously for 4 weeks. After treatment, the immunoperoxidase staining method was adopted to measure the changes of sex steroid receptors in entopic and ectopia endometrium. Results The entopic endometrium and the ectopia cyst were suppressed and atrophied dramatically in alarelin treated rats, profound suppression of explant volume was also occurred. The rate of inhibition in alarelin treated rats was(68±44)%. Estradiol receptor (ER) was obviously depressed within entopic endometrium in alarelin treated rats, and a negative correlation between ER in entopic endometrium epithelial cells and the inhibition rate was found. However, alarelin failed to result in obvious change neither in ectopia endometrial ER nor in entopic and ectopia endometral PR. Conclusion The alarelin causes dramatic atrophy and suppression of endometriosis, and the supressive effect on entopic endometrium in rat may be mediated by ER, while the mechanism involved in its inhibitory effect on endometrial implants may not be related to ER or PR. Measurement the changes of ER in entopic endometrium epithelial cells may do a favor in doping out curative effect on endometriosis.