Genotypic variants presented at miR-125b binding site correlated with varied CA125 levels in patients.

A. Serum CA125 levels in endometriosis patients were grouped by their rs1434536 genotypes. Bold lines indicate median values of CA125 levels in each genotype group. Notch regions define 95% confidence interval estimations of median values. B. Gene expression levels of BMPR1B and CA125 (MUC16) in ectopic endometrial tissues were measured by quantitative PCR for correlation study. C. Immunohistochemistry (IHC) staining was performed on ectopic endometrial tissues from patients. Anti-CD10 staining, a stroma cell-specific marker, serves as the reference to normalize immunointensity. Ectopic endometrium samples represented patients with rs1434536 genotype of CC, CT; and TT, as labeled on top of each column. A total of 6, 8 and 12 samples from tissue microarrays could be defined as TT, CT and CC genotype groups, respectively, and their expression levels for BMPR1B and CA125 were scored as described in method section (right panel). Box plot and ANOVA P value calculation were performed in R software suite.