Chemical profile and anti- inflammatory activity of the hydroethanolic extract of Cochlospermum regium (Schrank) Pilg. xylopodium

Chemical profile and anti- inflammatory activity of the hydroethanolic extract of Cochlospermum regium (Schrank) Pilg. xylopodium | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Chemical profile and anti- inflammatory activity of the hydroethanolic extract of Cochlospermum regium (Schrank) Pilg. xylopodium Jessica Araujo Isaias Muller, Bruna Fioravante Di Serio, Fabiana de Freitas Figueiredo, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8930105/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 10 Apr, 2026 Read the published version in Inflammopharmacology → Version 1 posted 8 You are reading this latest preprint version Abstract Background and Aim: Cochlospermum regium is widely distributed in the Brazilian Cerrado, where its xylopodium is traditionally used to treat inflammatory disorders. This study investigated the anti-inflammatory effects of the hydroethanolic extract of C. regium xylopodium (HECr) using in vivo and i n vitro models and characterized its phytochemical profile. Experimental Procedures: A 70% hydroethanolic extract was prepared from the xylopodium and subjected to phytochemical analysis. In vivo assays were conducted in male Swiss mice treated with HECr (25, 100, or 400 mg/kg) and evaluated in acetic acid-induced vascular permeability and lipopolysaccharide (LPS)-induced peritonitis models. Total and differential leukocyte counts and cytokine levels were determined in peritoneal fluid. In vitro , RAW 264.7 macrophages were used to assess cytotoxicity, nitric oxide (NO) production, and inflammatory cytokine release after LPS stimulation. Results: Phytochemical analysis identified myricetin-3-O-β-D-galactopyranoside, quinic acid, and quercetin-3-O-rhamnoside as major constituents. HECr significantly reduced Evans blue extravasation in the vascular permeability assay. In LPS-induced peritonitis, HECr decreased total leukocyte and neutrophil migration, reduced TNF-α and IL-1β levels, and increased IL-10 concentrations compared with vehicle-treated controls. In LPS-stimulated macrophages, HECr (1–20 µg/mL) reduced IL-1β and NO production, decreased TNF-α at lower concentrations, increased IL-13 levels, and showed no cytotoxicity. Conclusions: HECr exhibits significant anti-inflammatory activity, likely mediated by modulation of vascular permeability, leukocyte recruitment, cytokines, and NO, supporting its traditional use and potential therapeutic application. Cochlospermum regium phytochemistry inflammation leukocytes cytokines Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 10 Apr, 2026 Read the published version in Inflammopharmacology → Version 1 posted Editorial decision: Revision requested 10 Mar, 2026 Reviews received at journal 09 Mar, 2026 Reviewers agreed at journal 23 Feb, 2026 Reviewers agreed at journal 23 Feb, 2026 Reviewers invited by journal 23 Feb, 2026 Editor assigned by journal 23 Feb, 2026 Submission checks completed at journal 23 Feb, 2026 First submitted to journal 20 Feb, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8930105","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":596110276,"identity":"50d21277-7623-44e4-b4a0-1e8f34eafdbd","order_by":0,"name":"Jessica Araujo Isaias Muller","email":"","orcid":"","institution":"Federal University of Mato Grosso (UFMT)","correspondingAuthor":false,"prefix":"","firstName":"Jessica","middleName":"Araujo Isaias","lastName":"Muller","suffix":""},{"id":596110277,"identity":"49f0b1fe-c7c5-4f99-a65b-6e6d4c38ad63","order_by":1,"name":"Bruna Fioravante Di Serio","email":"","orcid":"","institution":"Federal 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This study investigated the anti-inflammatory effects of the hydroethanolic extract of \u003cem\u003eC. regium\u003c/em\u003exylopodium (HECr) using \u003cem\u003ein vivo\u003c/em\u003e and i\u003cem\u003en vitro\u003c/em\u003e models and characterized its phytochemical profile.\u003c/p\u003e\n\u003cp\u003eExperimental Procedures: A 70% hydroethanolic extract was prepared from the xylopodium and subjected to phytochemical analysis. \u003cem\u003eIn vivo\u003c/em\u003e assays were conducted in male Swiss mice treated with HECr (25, 100, or 400 mg/kg) and evaluated in acetic acid-induced vascular permeability and lipopolysaccharide (LPS)-induced peritonitis models. 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