Glyphosate affects the sporulation dynamics of Rhizophagus species more severely than mechanical defoliation or the contact herbicide diquat

Glyphosate affects the sporulation dynamics of Rhizophagus species more severely than mechanical defoliation or the contact herbicide diquat | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Glyphosate affects the sporulation dynamics of Rhizophagus species more severely than mechanical defoliation or the contact herbicide diquat Bérengère Bastogne, Catherine Buysens, Nicolas Schtickzelle, Ismahen Lalaymia, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4616972/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 11 Sep, 2024 Read the published version in Mycorrhiza → Version 1 posted 9 You are reading this latest preprint version Abstract Arbuscular mycorrhizal fungi (AMF) are totally dependent on a suitable host plant for their carbon resources. Here, we investigated under in vitro conditions, the impact of defoliation practices, i.e. mechanical defoliation or chemical defoliation with a contact (Reglone®, containing the active ingredient diquat) or systemic (RoundUp®, containing the active ingredient glyphosate) herbicide, on the dynamics of spore production of Rhizophagus irregularis and Rhizophagus intraradices associated to Solanum tuberosum and/or Medicago truncatula . Glyphosate affected the spore production rate more rapidly and severely than diquat or mechanical defoliation. We hypothesize that this effect was related to the disruption of the C metabolism in the whole plant combined with a possible direct effect of glyphosate on the fungus within the roots and/or in the soil via the release of this active ingredient from decaying roots. No glyphosate could be detected in the roots due to technical constraints, while its release from the roots in the medium corresponded to 0.11% of the active ingredient applied to the leaves. The three defoliation practices strongly affected root colonization, compared to the non-defoliated plants. However, the amount of glyphosate released into the medium did not affect spore germination and germ tube growth. These results suggest that the effects of defoliation on the dynamics of spore production are mainly indirect via an impact on the plant, and that the effect is faster and more marked with the glyphosate-formulation, possibly via a direct effect on the fungus in the roots and more hypothetically on spore germination in the environment. Arbuscular mycorrhizal fungi glyphosate diquat herbicide defoliant Full Text Additional Declarations No competing interests reported. Supplementary Files Supplementarydata.docx Cite Share Download PDF Status: Published Journal Publication published 11 Sep, 2024 Read the published version in Mycorrhiza → Version 1 posted Editorial decision: Revision requested 27 Jul, 2024 Reviews received at journal 22 Jul, 2024 Reviews received at journal 17 Jul, 2024 Reviewers agreed at journal 02 Jul, 2024 Reviewers agreed at journal 26 Jun, 2024 Reviewers invited by journal 24 Jun, 2024 Editor assigned by journal 24 Jun, 2024 Submission checks completed at journal 24 Jun, 2024 First submitted to journal 21 Jun, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4616972","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":327312080,"identity":"915d7551-2d91-42d2-996a-ee8ee1fc4777","order_by":0,"name":"Bérengère Bastogne","email":"","orcid":"","institution":"Université catholique de Louvain, Earth and Life Institute","correspondingAuthor":false,"prefix":"","firstName":"Bérengère","middleName":"","lastName":"Bastogne","suffix":""},{"id":327312083,"identity":"21f7e414-2c11-4e2b-b0d2-027e01b2d331","order_by":1,"name":"Catherine Buysens","email":"","orcid":"","institution":"Université 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