Enhanced Cellulose Isolation from Sugarcane Bagasse through Sequential Alkali and Oxidative Treatment

Enhanced Cellulose Isolation from Sugarcane Bagasse through Sequential Alkali and Oxidative Treatment | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Enhanced Cellulose Isolation from Sugarcane Bagasse through Sequential Alkali and Oxidative Treatment shikha Kumari, Dinesh Arora, Manjeet Kaur, Geeta Dhania This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7360666/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 6 You are reading this latest preprint version Abstract As the world seeks sustainable alternatives to fossil-based materials, agricultural residues like sugarcane bagasse are gaining prominence as valuable bioresources. Sugarcane bagasse, the fibrous waste left after juice extraction, is an agricultural residue that is particularly rich in cellulose, a biopolymer with immense potential for green material innovation. The present work utilised sugarcane bagasse, to obtain cellulose by optimising the Sodium hydroxide (NaOH) and Sodium hypochlorite (NaOCl) concentrations. Characterisation techniques such as Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscope (FE-SEM) and X-ray Diffraction (XRD) were employed for detailed analysis. This article also encloses the effect of NaOH and NaOCl concentration on the cellulose yield and the various possible applications of the obtained cellulose. To evaluate statistical significance, one-way ANOVA was performed, complemented by Tukey’s multiple comparison test. Compositional analysis showed that the cellulose content of raw sugarcane bagasse was 42.6±1.5%. However, after alkali and bleaching treatment, the cellulose content was in the range 42.6±0.6% - 56.5±0.5%. FTIR analysis confirmed the successful cellulose extraction from sugarcane bagasse, as evidenced by the disappearance of lignin and hemicellulose associated peaks and characteristic cellulose absorption bands. XRD analysis revealed an increase in the crystallinity index from 29.8% in SCB to 53.7% in extracted cellulose. Morphological analysis employing FE-SEM highlighted significant surface differences in SCB and extracted cellulose. Statistical analysis unfolded that amendment with NaOH and NaOCl enhanced the cellulose yield significantly (p≤0.05) compared to raw sugarcane bagasse. The study highlights the immense potential of agricultural waste as a renewable and cost-effective source of cellulose. By leveraging these residues, industries can reduce dependence on conventional raw materials while promoting sustainable and environmentally responsible practices. Agricultural residue Cellulose Sugarcane Bagasse Alkaline treatment Bleaching Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Introduction The rapid generation of agricultural waste has become a growing environmental concern. It often leads to open-field burning as a method of disposal and this practice significantly contributes to greenhouse gas emissions and air pollution, exacerbating environmental and health issues (Ben et al. 2024; Debnath et al. 2021 ). This agricultural waste is an eco-friendly renewable resource and a natural carbon source; it is especially well-suited to be utilised as a precursor to create novel carbon-based materials (Sole et al. 2021 ). Despite its potential, less than 5% of LCB is currently utilized for productive purposes, with the rest remaining underexploited or discarded (Ashok et al. 2022). Effective utilization of agro-waste plays a vital role in reducing environmental harm while promoting the development of sustainable materials. In particular, the valorization of lignocellulosic biomass (LCB) offers a promising solution by transforming abundant agricultural residues into value-added products. This approach not only addresses the issue of waste management but also helps mitigate environmental pollution and decrease dependence on fossil fuel-based resources (Gangil & Wakudkar, 2013 ). By harnessing LCB for the production of eco-friendly materials, significant progress can be made toward achieving environmental sustainability and advancing a circular bioeconomy. Lignocellulosic biomass (LCB), composed mainly of cellulose, hemicellulose, and lignin, can be sourced from sugarcane bagasse, wood chips, sawdust, rice husks, cotton linens, etc., highlighting its vast availability in nature (Khui et al. 2021 ). Among the components of LCB, cellulose stands out due to its biodegradability, renewability, and its ability to be engineered into micro- and nanoscale particles that exhibit novel physicochemical properties (Shao et al. 2020). The growing interest in cellulose is due to its potential applications in biodegradable plastics, textile industry, biomedical devices, drug delivery, and landscape mulch films (Raymond et al. 2024). Harnessing cellulose from agricultural waste such as sugarcane bagasse offers a dual benefit—efficient waste management and the development of eco-friendly materials for a greener future. Waste products from agriculture, such as can be used as cheap, sustainable, and renewable raw materials to make cellulose and its derivatives for the Vast amounts of sugarcane bagasse waste are produced annually by processing sugarcane to make juice and alcohol (Saad et al. 2022 ). The accumulation of massive quantities of sugarcane bagasse poses a complex waste problem because, up until now, it scarcely found any commercial utilisation (Shaikh et al. 2009 ). Managing agricultural biomass has been regarded as an essential tactic in managing and using natural resources and maintaining environmental quality (Raymond et al. 2024). Nowadays, the sugar industry uses the majority of sugarcane bagasse as boiler fuel, feedstock, and manure. Nevertheless, the residual bagasse threatens the ecosystem (Mubarak et al. 2024 ). SCB primarily consists of cellulose, which is manufactured in enormous quantities worldwide (Mahmud and Ananya, 2021). According to its chemical makeup, Cellulose is a natural linear polymer composed of anhydroglucose units connected through β-glycosidic bonds between the first and fourth carbon atoms (Kadla et al. 2000). Cellulose structure is shown in Fig. 1 . It is arranged into fibrils encased in a lignin and hemicellulose matrix. Although cellulose II chains are anti-parallel, cellulose I has a parallel chain orientation. The efficient use of sugarcane bagasse (SCB) as raw material for cellulose extraction has become more prevalent in recent years (Sun et al. 2004 ). Cellulose is used extensively as a diluent, thickener, cellulose ether, bioplastics, lubricant, regenerated textile fibres, film binder, and coating in the production of tablets and capsules (Wan et al. 2018; Aziz et al.2022). Various pretreatment techniques, generally divided into four primary categories: physical, chemical, physicochemical, and biological, can be used to separate cellulose (Sun et al. 2016 ). Among the different pretreatment techniques, chemical procedures use chemicals to dissolve hemicelluloses and break the bond between cellulose and lignin. This strategy has several benefits, such as a simple extraction procedure, high extraction efficiency, superior thermal stability, advantageous crystallinity, simplicity in regulating reaction conditions, and cost-effectiveness (Lou et al. 2022 ). Bagasse's primary benefit is that it is a waste material that yields a very cost-effective, fully or partially biodegradable product after a few pretreatments, a crucial consideration in today's market. Furthermore, with the correct procedure, the extracted fibre can exhibit reasonably acceptable mechanical properties (Loh et al. 2013 ). Numerous investigations have explored the extraction of cellulose from agro residue through chemical treatments. For instance, Mubarak et al. 2024 utilised 200 ml (10–30%) of NaOCL to extract cellulose from 5g Sugarcane bagasse. However, this concentration of bleaching agent seems so high for a meager portion of bagasse. In another study, cellulose extraction from SCB employed 2–18% NaOH and 38% H 2 O 2 (Rasheed et al. 2024 ). Moubarik et al. ( 2013 ) used a two-stage treatment including hot water and NaOH(15%) for cellulose extraction from SCB, yielding 42% of cellulose. No bleaching treatment was given here, and the NaOH(15%) concentration was high. Also, the obtained yield was not much higher than that of raw sugarcane bagasse. Thus, it is discernible that most of the researchers used a high concentration of NaOH and bleaching agents. The present study attempts to find the optimum conditions for cellulose extraction. Also, it focuses on the effect of different sodium hypochlorite and sodium hydroxide concentrations on the yield and properties of extracted cellulose. The extracted cellulose was characterised with the help of FTIR, XRD and SEM. Through a straightforward recycling method that relies on removing the non-cellulosic component from SCB, the current work seeks to increase the economic value of sugarcane bagasse. Materials and Methodology Materials and chemical substances Raw sugarcane bagasse (SCB) was gathered from juice merchants in the Indian state of Haryana's Rohtak area. Sodium hypochlorite from Loba Chemie was used as a bleaching agent. CDH provided sodium hydroxide and hydrogen peroxide. All chemical compounds used in this experimental study were of analytical grade. Raw Material Preparation To guarantee total cleanliness, the substrate was cleaned adequately with running tap water to remove dirt and then sun-dried. Following this, the substrates were ground and sieved with a sieve of size 0.2 mm (Yadav et al. 2024 ). The sieved SCB powder was stored in a ziplock container at room temperature. Determination of the Chemical composition of Sugarcane bagasse The composition of SCB affects the bioconversion processes. A significant proportion of sugarcane bagasse consists of cellulose, cemicellulose and lignin. Apart from these compositional ingredients, it includes other elements like extractives, moisture, nitrogen, organic carbon, ash, and trace elements (Alokika et al. 2021 ). The SCB in this study was analysed for extractive, lignin, holocellulose, cellulose, and hemicellulose content. The process diagram for the compositional analysis of Sugarcane bagasse is shown in Fig. 2 . Extractives In the Soxhlet extractor setup, 150 ml of acetone and 2.5 g of SCB were taken. During a 4-hour run period, the residence periods for the boiling and rising stages were meticulously set to 70 and 25 minutes, respectively, on the heating mantle. The extracted sample was allowed to air dry for a few minutes at room temperature. The removed material's weight remained constant in a convection oven set at 105°C. The extractive content can be calculated using Eq. ( 1 ) ( TAPPI T 204 cm-17). $$\:\text{E}\text{x}\text{t}\text{r}\text{a}\text{c}\text{t}\text{i}\text{v}\text{e}\text{s}\left(\text{%}\right)=\frac{W1}{W}x100$$ 1 Where W = Initial weight of SCB and W1 = Final weight obtained after treatment. Lignin The Tappi T222 om-88 (Tappi test methods) determines the amount of lignin in biomass samples. 1 g of extractive-free biomass and a 72% H 2 SO 4 solution were heated for two hours at room temperature. After diluting the sample with water to bring the sulphuric acid concentration down to 3 per cent, it is boiled for an additional four hours. The lignin is then filtered after being given time to settle. The residue is rinsed with hot water until a neutral pH is achieved. The extracted lignin content can be determined using Eq. ( 2 ) (Rizwan et al. 2021 ). $$\:Lignin\left(\%\right)=\frac{W3}{W2}x100$$ 2 Where, W2 = Initial sample mass and W3 = Obtained sample mass. Holocellulose With a few modifications, the conventional procedure outlined by Wise et al. ( 1946 ) was used to determine the holocellulose content. 10 g of SCB was bleached using hydrogen peroxide buffered with sodium hydroxide solution. The mixture was heated at 70°C for 4 hours. Once the mixture had cooled, the residue was filtered out, cleaned with water, dried, and weighed. Hollocellulose, a blend of cellulose and hemicellulose found in residues, can be quantified using Eq. ( 3 ) (Rizwan et al.2021). $$\:\text{H}\text{o}\text{l}\text{o}\text{c}\text{e}\text{l}\text{l}\text{u}\text{l}\text{o}\text{s}\text{e}\left(\text{%}\right)=\frac{\text{W}5}{\text{W}4}\text{x}100$$ 3 Where W4 = Weight of the original sample, and W5 = Weight of the obtained holocellulose. Cellulose For cellulose content determination, a 1 g sample of holocellulose and 25 ml of 17.5% NaOH were combined in a flask and heated at 95°C for one hour. 25 millilitres of distilled water (DW) was added, and the residue was filtered. After that, 25 millilitres of a 10% acetic acid solution was added, filtered once more, and repeatedly cleaned with distilled water. The amount of cellulose residue was determined after the sample was dried for 24 hours at 40°C. Silva et al . (2011). Cellulose content percentage is determined using Eq. ( 4 ) (Song et al. 2019 ). $$\:\text{C}\text{e}\text{l}\text{l}\text{u}\text{l}\text{o}\text{s}\text{e}\left(\text{%}\right)=\frac{\text{W}7}{\text{W}6}\text{x}100$$ 4 Where = Initial amount of holocellulose and W7 = Final amount of extracted cellulose. Hemicellulose The difference between the holocellulose and cellulose concentrations was calculated to determine the hemicellulose fraction (Eq. 5) (Balasubramani et al. 2024 ). The percentage that remains after deducting cellulose from holocellulose is hemicellulose. This formula is frequently used to analyse the composition of plant fibres in wood science, biomass research, and the paper industry. Hemicellulose = Holocellulose − Cellulose (5) Isolation of Cellulose In this study, cellulose extraction was conducted with the help of combined and modified methods of Ungprasoot et al. ( 2021 ), Melesse et al. ( 2022 ) and Khiewsawai et al. (2023). The process of cellulose extraction from sugarcane bagasse is shown in Fig. 3 , and it mainly involves three steps: dewaxing using ethanol, alkaline treatment using NaOH and bleaching treatment using NaOCl. The cellulose yield(%) is greatly affected by the amount of NaOH and NaOCl used. Thus, the two are used in varying concentrations, and six samples were prepared and labeled accordingly as given in Table 1 . Table 1 Chemical Processing Conditions for Cellulose Extraction from Sugarcane bagasse. Sample NaOH (%) NaOCl (%) SCBC1 2 1.5 SCBC2 2 3 SCBC3 5 1.5 SCBC4 5 3 SCBC5 8 1.5 SCBC6 8 3 Dewaxing Dewaxing was done using a solution of ethanol and deionised water in 1:1 v/v for around four hours, then boiling the mixture for 1.5 hours. This procedure was repeated twice to remove dust, dirt, sugar extracts, and water-soluble contaminants. To remove any remaining sugar and surfactants, the mixture was rinsed with hot water first, followed by cold water. After that, it was oven-dried for a whole day. Alkaline Treatment Three varying concentrations 2%, 5%, and 8% of sodium hydroxide (NaOH) solution(200 ml ) were applied to a 10 g sample to perform the alkaline treatment. The samples were heated for two hours, stirring occasionally, to a temperature of 75°C. This process made the desired changes possible, guaranteeing that the sample and the alkaline solution interacted well. They were then extensively cleaned with distilled water to neutralise the solid residue that had been produced. Bleaching Two hundred millilitres of NaOCl at two differing concentrations 1.5% and 3% was used to bleach the residue left over after the alkaline treatment. For 30 minutes, residue obtained after alkali treatment was heated to 80°C while being constantly stirred and then to attain a neutral pH it was cleaned with distilled water. Then for 16 hours, the isolated cellulose samples were oven-dried at 50°C. After oven drying, it was ground into a fine powder and kept in ziplock containers to retain moisture. Yield Cellulose yield indicates the proportion of cellulose successfully extracted, reflecting cellulose recovery efficiency. The yield was calculated as a percentage relative to the dry weight of the SCB after treatments. Accurate measurements of the initial and final weights of SCB and SCBC were used to determine the yield using Eq. ( 6 ) (Mubarak et al., 2024 ). $$\:\text{Y}\text{i}\text{e}\text{l}\text{d}\left(\text{%}\right)=\frac{\text{W}9}{\text{W}8}\text{x}100$$ 6 W8 = Initial weight of SCB, and W9 = Final weight of extracted cellulose. Characterization FTIR Analysis Fourier transform infrared (FTIR) Spectroscopy was used to examine the functional groups. Bruker's Invenio ® FTIR spectrometer was employed. It had a 2 cm − 1 resolution and was operated in gearbox mode. FTIR spectra were obtained over a range of wavenumbers from 4000 to 400 cm − 1 . XRD A multifunctional, adaptable X-ray diffractometer system of model Smartlab 3kW/Rigaku was used to conduct the X-ray diffraction analysis. With a phase of 0.04 and a scanning time of five minutes, the analysis was carried out in the 2β range value scanned from 10 to 50º. Segals method [(Eq. (7)] was used to calculate crystallinity index (Segal et al. 1959). CrI (%) = [(I002 - Iam) / I002] × 100 (7) Where, I002 = Intensity of the crystalline peak and Iam = Intensity of the amorphous background FE-SEM The micrographs were obtained using a Carl Zeiss field emission scanning electron microscope. The aluminium stubs were covered with a layer of gold after the dried, finely powdered SCB and SCBC3 samples were put on them. Better conductivity is guaranteed by this gold coating, which also improves the quality of the images acquired during the ensuing image processing procedure. Statistical analysis The experimental work was performed in triplicates. The results are given as the mean value and its corresponding standard deviation. The mean of altered treatments, Dunnett test for control, and analysis of variance (ANOVA) were assessed using Tukey’s test at the 0.5% statistical level in the IBM SPSS Statistics 25.0. Statistically significant differences among treatments are indicated by different letters in the figures, based on the Tukey multiple comparison test (p ≤ 0.05). Graphs were generated using Origin software. Results and Discussion Chemical Composition of Raw Sugarcane Bagasse The raw SCB employed in this investigation contains 7.4 ± 0.9% extractives, 16.3 ± 0.5% lignin, 23.6 ± 2.08% hemicellulose, and 42.6 ± 1.5% cellulose. The chemical composition of SCB is shown in Fig. 4 . The sugarcane bagasse (SCB) used in the aforementioned study has a cellulose, hemicellulose, and lignin concentration that falls within the ranges described in prior investigations. The cellulose content was close to that of Yadav et al. 2024 , where the cellulose content was 43.2%. Nonetheless, they reported 16.6% and 26.5% of lignin and hemicellulose respectively. The extractive and cellulose content of the present study were higher than that of the Rasheed et al. 2024 , where 6% extractives and 41% cellulose were reported. The cellulose concentration was lower than the studies of Mohammed et al. 2023 and Ungprasoot et al. 2024 where sugarcane bagasse consists of 46% and 47% cellulose, respectively. In comparison to other agroresidues, the cellulose content was higher than wheat husk (36–39%) and lower than rice straw (48.5%) (Bledzki et al. 2011; Ungprasoot et al. 202). The lignin content of 23% (Lalucea et al. 2019), 21% (Rana et al. 2021 ) and 38% (Mubarak et al. 2024 ) were also found to be higher than in the current study, where lignin content is 16.3 ± 0.5%. The hemicellulose content was also higher in the few studies, ranging from 26.5%-35.2% (Laluce et al. 2019 ; Ungprasoot et al. 2021 ; Yadav et al. 2024 ). Table 2 shows the comparative analysis of the chemical composition of SCB in the present study, as well as the chemical composition of SCB and different agricultural residues reported in the literature. Overall, the compositional data of sugarcane bagasse (SCB) closely aligns with values reported in the literature, highlighting its potential as a promising feedstock for various bio-based applications. Its well-balanced proportions of cellulose, hemicellulose, and lignin, combined with a moderate level of extractives, make SCB highly suitable for uses such as biofuel production, paper manufacturing, and the development of composite materials. Table 2 Chemical composition of SCB and different agricultural residues. Agro-residue Cellulose (%) Hemicellulose (%) Lignin (%) Extractive (%) Reference SCB 42.6 ± 1.5% 23.6 ± 2.08% 16.3 ± 0.5% 7.4 ± 0.9% Present Study SCB 46 28.7 22.6 2.04 Silva et al. (2011) SCB 40 35 23 - Laluce et al. ( 2019 ) SCB 45.6 29.4 21.2 - Rana et al. ( 2021 ) SCB 41 18 16 6 Rasheed et al. ( 2024 ) SCB 43.2 26.5 16.6 - Yadav et al. ( 2024 ) SCB 47 20.8 28 - Ungprasoot et al. ( 2021 ) SCB 46 16 38 - Mubarak et al. ( 2024 ) Wheat Husk 36–39% 18–21% 16% - Bledzki et al., ( 2010 ) Wheat Bran 24 32 46 - Stevenson et al. ( 2012 ) Wheat Straw 33–45 19–32 8–16 - Wang et al. ( 2012 ) Calotropis procera 64.1 ± 1.6 19.5 ± 1.2 9.7 ± 1.2 - Song et al. ( 2019 ) Rice straw 48.5 35.2 5 - Ungprasoot et al. ( 2021 ) Chickpea husk 52.3 31.9 13.9 1.8 Lamo et al. ( 2024 ) Impacts of alkali and bleaching treatment on the Cellulose yield. This study examined the impact of varying sodium hydroxide and sodium hypochlorite concentrations on sugarcane bagasse cellulose production. It is generally recognised that hemicellulose is dissolved and removed from the biomass due to pretreatment with NaOH causing delignification, by significantly rupturing the cross-ester linkage between complex hemicellulose and lignin (Jung et al. 2020 ). According to Hashim et al. ( 2017 ), the lignin and hemicellulose dissolve in alkali solutions, forming a black liquid when the hydrogen bonds between the lignocellulosic components are broken, increasing the yield of cellulose. After alkali treatment, hemicellulose and lignin were still present in the extracted residue, and bleaching was required to eliminate them further (Geng and Han, 2023 ). Oxidising chemicals, such as sodium hypochlorite bleaching, were used to remove leftover lignin and other impurities affecting cellulose appearance and quality (Kapdi et al. 2024 ). The hypochlorite ion in NaOCl is a potent oxidant that can dissolve the ether bond in the lignin structure and enhance the pulp's white brightness (Sayakulu and Soloi, 2021). The brightness and purity of the cellulose were further enhanced by bleaching. Alkali pretreatment caused SCB's cellulose content to rise while hemicellulose and lignin levels were reduced. The pictorial representation of the various extracted cellulose samples can be seen in Fig. 5 . It was discovered that the yields for SCBC1 and SCBC2 were 46.1 ± 0.7% and 43.3 ± 0.6%, respectively. These are significantly less than the yield of 57.6% obtained in the study by Yadav et al. 2024 . Hemicellulose can be partially broken down by 2% concentrations of NaOH, which results in some hemicellulose being removed from the sugarcane bagasse. Nonetheless, the pretreated biomass might still include a sizable amount of hemicellulose (Yadav et al. 2024 ). The use of NaOCl for bleaching could be the cause of the decreased concentration. It is evident from the study of Raymond et al. 2024 that a high concentration of bleaching agent reduced the yield of cellulose. In their study, using 10–20% NaOCl resulted in a lower yield, i.e., 32.2–40.2%. In another study 6% NaOCl alone was used for cellulose extraction, and the yield was 48% (Laluce et al. 2019 ). Thus, it is clear that the NaOCl concentration also affects the cellulose yield. Sample SCBC 3 produced yield of 56.5 ± 0.2%. The Kapdi et al. ( 2024 ) study also demonstrated similar outcomes, with a 58% cellulose production from rice straw. The yield for SCBC 4 was 53.1 ± 0.4%. The cellulose yield was reduced significantly at high concentrations of NaOH (8%). SCBC5 and SCBC6 were 43.6 ± 0.8% and 42.6 ± 0.6% (Lowest Yield) respectively. The results are in harmony with the results of (Rasheed et al. 2024 ), where the yield using 10% NaOH and NaOCl was 44.2%. The yield percentage of extracted cellulose samples is shown in Fig. 6 . It was discovered that raising the sodium hydroxide concentration reduced the yield because some of the cellulose chains would break down during the treatment procedure. The cellulose molecules were freely distributed in the solvent (NaOH) because of their stable open structure. A high concentration of NaOH will break up a few crystalline regions in cellulose and cause it to dissolve more readily in the solution treatment, lowering the amount of cellulose fibre produced (Azmin et al. 2020 ). A certain quantity of cellulose may dissolve in high concentrations of NaOH, lowering the percentage of cellulose (Martin-Bertelsen et al. 2020 ). This investigation's findings align with those of Kathirselvam et al. ( 2019 ), Melesse et al. ( 2022 ), Rahayu et al. ( 2022 ), and Saad et al. ( 2022 ), who also showed that a higher concentration of NaOH resulted in decreased cellulose yield. NaOCl's bleaching qualities enhanced the colour of the extracted samples and aided in the dissolution of any remaining lignin and hemicellulose. SCBC1 was light brown, possibly due to the low concentration of NaOCl (1.5%). SCBC2, SCBC3 and SCBC5 were found to be pale creamish yellow, whereas SCBC4 and SCBC6 were much brighter and white in appearance. Overall, Bleaching makes the finished cellulose product more aesthetically attractive and appropriate (Kapdi et al. 2024 ). It can be concluded that much higher and lower concentrations result in low yield and purity, respectively. Thus, the optimum concentration for cellulose extraction were 5% NaOH and 1.5% NaOCl, resulting in highest yield 56.5 ± 0.2% (SCBC3). The obtained cellulose yield reflects an effective isolation procedure and aligns well with previously reported values, further supporting the potential of sugarcane bagasse in value-added bioproduct development. FTIR Sugarcane bagasse (SCB) and extracted cellulose can be efficiently characterised using Fourier Transform Infrared (FTIR) spectroscopy. It aids in elucidating modifications to functional groups and molecular structures brought about by the pre-treatment procedure.The spectra of SCB and all extracted samples were broadly similar, with a few significant differences that indicate structural and compositional changes. Table 3 unfolds the Functional groupspresent in the FTIR spectra of Sugarcane bagasse and Sugarcane bagasse cellulose. The broad peak at 3326 cm⁻¹ and 3338–3339 cm⁻¹ in raw SCB and all the extracted cellulose samples, respectively, confirms the presence of hydroxyl (-OH) groups, indicating strong hydrogen bonding, a characteristic of cellulose (Nandiyanto et al. 2019 ). As the purification process advanced, the bell-shaped absorption band between 2980 and 3690 cm⁻¹ became somewhat narrower (Wang et al. 2018 ). Similar peaks at approximately 3434.5 cm⁻¹ and 3426.1 cm⁻¹were found for the bagasse and the extracted cellulose, respectively (Lu et al. 2012). Figure 7 presents the FTIR spectra of Raw SCB and extracted cellulose samples. The peak at 2890–2902 cm⁻¹in extracted cellulose samples and SCB were associated with C–H stretching from polysaccharides, validating the sample's organic composition by matching the C–H stretching vibrations of the methyl and methylene groups in polysaccharides (Saad et al. 2022 ; Mubarak et al. 2024 ). Table 3 Functional groups in the FTIR spectra of Sugarcane bagasse and Sugarcane bagasse cellulose. Wavenumber (cm − 1 ) Functional group assignment Sugarcane bagasse Sugarcane bagasse cellulose 3326 3338–3339 O-H Stretching vibrations 2890 2890–2902 C-H Stretching 1723 - Carbonyl (C = O) stretching 1630 1612–1630 OH (Water Absorbed) 1511 - Aromatic C = C Stretching 1292 - Aromatic C-O Stretching 1152 − 1015 1123–1167 C-O-C Stretching 895 982–1018 C-O Stretching Vibration (Cellulose) - 816–841 Ring Deformation 653 622 − 594 C-H Bending The H–O–H bending of absorbed water is responsible for the peak at 1612–1630 cm⁻¹, indicating that the SCB and extracted cellulose contain some moisture. The moisture content of cellulose is marked by this area in other studies by Wang et al. ( 2020 ), Saad et al. ( 2022 ), Freitas et al. ( 2022 ) and Mubarak et al. ( 2024 ) with peaks in the range 1629 cm⁻¹to 1645 cm⁻¹. Further Peaks in the 1123–1167 cm⁻¹and 1018–982 cm⁻¹ ranges are present in extracted cellulose and in the range 1152 cm⁻¹-1015 cm⁻¹ and at 895 cm⁻¹ in SCB confirming C–O–C and C–O stretching respectively, which are traits of cellulose's glycosidic bonds which are crucial cellulose structural elements. The polysaccharide structure is confirmed by the literature, which shows that absorption bands in the 1162 cm⁻¹-899 cm⁻¹ range are linked to C–O–C and C–O stretching vibrations (Sayed and Khalaf, 2023 ; Yadav et al. 2024 ). SCB peaked at 653 cm⁻¹, corresponding to C–H bending. All the extracted cellulose samples exhibited Peaks at 816–841 cm⁻¹ and 622–594 cm⁻¹, and are associated with ring deformation and C–H bending, common in cellulose structures. These results align with the literature (Mubarak et al. 2024 ; Freitas et al. 2024 ). Raw SCB exhibited peaks at 1723 cm⁻¹, 1511 cm⁻¹ and 1292 cm⁻¹, which correspond to C = O stretching that represents aldehyde, ketone, or carboxylic acids in hemicellulose, aromatic C = C stretching of lignin and C-O Stretching vibrations of lignin and hemicellulose, respectively. These peaks were either absent or were reduced in the extracted cellulose samples, which indicates the removal of hemicellulose and lignin, suggesting that cellulose has been successfully separated from sugarcane bagasse. Previous studies demonstrated similar findings (Viera et al. 2007 ; Zhao et al. 2018 ; Kapdi et al. 2024 ).Identifying the extracted sample as cellulose is strengthened by the close alignment of FTIR data with known cellulose spectrum features. While the water-related peak indicates some bound moisture, hydroxyl groups, glycosidic connections, and C–O stretching validate the cellulose structure. The FTIR peaks show that lignin and hemicellulose have been significantly removed. Typically, The current samples peak at 1612–1630 cm⁻¹, not the aromatic C = C stretching of lignin but rather H–O–H bending (absorbed water). The presence of cellulose is confirmed by the prominent peaks in your spectrum for O–H stretching (3338 cm⁻¹), C–O–C glycosidic linkages (1123–1167 cm⁻¹), and C–O stretching (1018 − 982 cm⁻¹). Rather than lignin or hemicellulose, the peaks at 816–841 cm¹ and 622–594 cm¹ are characteristic of cellulose. The presence and successful isolation of cellulose are confirmed by the preserved C–O–C and C–H peaks. Overall, the work was in harmony with (Sun et al. 2004 ; Viera et al. 2007 ; Perumal et al. 2018 ; Laluce et al. 2019 ; Katakojwala and Mohan 2020 ; Rasheed et al. 2024 ; Freitas et al. 2024 ). FTIR data indicates that the sugarcane bagasse has successfully undergone hemicellulose removal and delignification, leaving behind purified cellulose. XRD X-ray diffraction (XRD) is a crucial method for figuring out a material's crystallographic structure. This method includes subjecting a material to X-ray radiation and then determining the angles and intensities of X-ray scattering that result (Mubarak et al. 2024 ). Cellulose, lignin, and hemicellulose make up most of SCB; cellulose is crystalline, whereas lignin and hemicellulose are amorphous (Kundu et al. 2023 ). The present study examined structural changes and assessed the impact of alkali (NaOH) and bleaching (NaOCl) on the crystallinity of the resultant cellulose using XRD analysis of raw sugarcane bagasse (SCB) and SCBC3, which yielded the maximum amount of cellulose. The XRD spectra of SCB and SCBC3 are shown in Fig. 8 . There were discernible peaks at 2θ values of 15.16° and 21.96° in the XRD analysis of SCB, indicating the existence of the cellulose I. SCBC3 displays prominent peaks at 2°, measuring 15.84° and 22.68°, significantly stronger than in the bagasse. The existence of these peaks is proof that the treatments affected the extracted cellulose. These peak positions imply that the treated fibres' interlunar distance had grown (Liu et al. 2019 ). The crystallinity index was 29.8% and 53.2% for SCB and SCBC3, respectively. Due to its higher amorphous proportion of lignin and hemicellulose, the SCB has low relative crystallinity (Guilherme et al., 2013). The alkali (NaOH) and bleaching (NaOCl) treatment are the causes of the enhanced crystallinity index seen in extracted cellulose (Melesse et al. 2022 ). Alteration in structure and crystallinity of cellulose may be caused as a result of disruption of intra- and inter-chain H-bonding of cellulosic fibrils (Sharma et al. 2023 and Kundu et al. 2023 ). However, mild reaction conditions did not change the crystalline structure of the cellulose, and the extracted cellulose constituted mainly cellulose I in the structural form (Saad et al. 2022 ). The finding of current study is consistent with earlier studies that found that extracting cellulose from sugarcane bagasse increased the crystallinity index. In a study by Yadav et al. ( 2024 ), XRD patterns continuously show peaks at roughly 2θ angles of 16.3 and 22.5°. Compared to the untreated SCB biomass, the SCB that had received sodium hydroxide pretreatment displayed a significantly raised peak. The CrI value of the raw SCB was 38.8%. Extracted celulose produced CrI levels of 53.64%. Saad et al. ( 2022 ) discovered that the bagasse fibre exhibits two distinct peaks around the 2θ value of 15.33º and 22.09° and the extracted cellulose showed prominent peaks at 2θ of 16.35º and 22.47º that were noticeably stronger. Also the CrI raised from 31.76–51.13%. The presence of the cellulose I structure is suggested by the peaks that can be seen in the bagasse fibre XRD analysis at 2θ values of 15.22° and 21.94°. Similarly, the extracted cellulose displays distinct peaks at 2°, measuring 15.02° and 22.19°, which are significantly more potent than those in the bagasse (Mubarak et al. 2024 ). The results of this study are also in agreement with several previous studies that reported an increase in this index value after biomass pretreatment (Galiwangoa et al. 2019;Kininge and Gogate 2022 ; Melesse et al. 2022 ; Bangar et al. 2023 ; Sharma et al. 2023 ; Sayed & Khalaf 2023 ; Lamo et al. 2024 ; Freitas et al.2024; Rasheed et al. 2024 ). This discovery highlights how alkali treatment affects the cellulose fibres' structural characteristics, which advances our knowledge of their properties and uses. FE-SEM The morphological differences between SCB and the extracted cellulose sample SCBC3 treated were analysed using Field emission scanning electron microscopy (FE-SEM), as presented in Fig. 9 . The FE-SEM image of raw sugarcane bagasse reveals a compact, smooth, and intact surface with minimal visible porosity. This dense outer layer is characteristic of the naturally occurring lignocellulosic matrix, composed predominantly of cellulose, hemicellulose, lignin, waxes, and other extractives (Kumar et al. 2014 ; Khalid et al. 2021 ). In contrast, the treated sugarcane bagasse cellulose shows a highly porous, fibrillated structure, indicative of effective removal of non-cellulosic components, primarily lignin and hemicellulose (Feleke et al. 2023 ; Ding et al. 2025 ). Similar changes were seen in the morphological structure of SCB, followed by alkali pretreatment by Yadav et al. ( 2024 ). The untreated sample retains intact plant cell wall architecture, with minimal evidence of fibril exposure, suggesting low surface accessibility and reactivity. Conversely, the treated cellulose sample exhibits disrupted cellular networks and partially exposed cellulose microfibrils, suggesting significant structural breakdown and loosening of the plant matrix due to chemical pretreatment (Cao et al. 2006 ). A lack of visible porosity in the raw sample indicates the hydrophobic nature and inaccessibility of cellulose chains in their native state. In contrast, the treated sample presents a network of interconnected pores, cracks, and voids, beneficial for applications requiring water absorption or bonding (Velmurugan et al. 2024 ). The increased porosity directly correlates with improved functional properties, such as water uptake, surface modification, and biopolymer reinforcement (Razali et al. 2022 ). Overall FE-SEM analysis confirms that pretreatment of sugarcane bagasse leads to profound morphological changes, especially in surface porosity (Rasheed et al. 2024 ), fibrillation (Phiri et al. 2024 ) and accessibility(Ding et al. 2024), facilitating enhanced surface area (Verma and Goh 2021 ), which is crucial for downstream applications. The results of the current study are also in harmony with the previous studies for SCB treated with acidified NaOCl and alkali treatment by Kumar et al. ( 2014 ), with acid and alkaline pretreatment and combined hydrothermal and alkaline pretreatment by Guilherme et al. ( 2015 ), with bleaching agent by Mubarak et al. ( 2024 ), with alkaline treatment and hydrogen peroxide bleaching by Rasheed et al. ( 2024 ). Additionally, these findings also show consistency with the results of FTIR and XRD, and validate the efficiency of the cellulose extraction process. Statistical analysis Results of statistical analysis revealed that the yield of cellulose was significantly enhanced (p ≤ 0.5) in SCBC1, SCBC2, SCBC3, SCBC4, and SCBC5 after the amendment with NaOH and NaOCl compared to raw sugarcane bagasse. Meanwhile, SCBC6 did not exhibit a significant change in yield. It was also observed that no significant (p ≤ 0.5) difference were observed between SCBC2 and SCBC5. Application An intriguing polymeric substance that is widely available on Earth is cellulose and agricultural leftovers are good source of cellulose. It is an auspicious raw material for replacing non-renewable feedstocks because of its widespread availability. Researchers have focused on natural cellulose because of its advantages over synthetic cellulose, which include its availability, affordability, perusability, biocompatibility, and minimal toxicity. It can also undergo chemical modification to enhance its chemical and/or physical characteristics. Commonly appealing uses of cellulose include printing, electronics, packaging, and healthcare materials (Aziz et al. 2022 ; Magalhaes et al. 2023). Various applications of cellulose are presented in Fig. 10 . The literature contains a wealth of research on modifying and using cellulosic materials. The efficient incorporation of SCB cellulose into a hydrogel composite using the gamma irradiation approach was noteworthy. This composite can be utilized as a platform for the adsorption of mercury ions (Khiewsawai et al. 2024 ). Cellulose was converted to carboxymethylcellulose (CMC) in a different study (Ungprasoot et al. 2021 ). Cellulose extracted from SCB was used to prepare microfibrillated cellulose (MFC). This improved the properties of microfibrillated cellulose, allowing it to be easily shaped into pliable sheets. The increased flexibility provides a straightforward method for creating eco-friendly, sustainable and biodegradable products (Wibowo et al. 2024 ). The conversion of cellulose into hydroxyethyl cellulose has numerous critical industrial applications (Halim, 2014). Using sugarcane bagasse, Alirach et al. ( 2023 ) created and characterised bio-sheets for possible application as bio-sheet packaging material. The biosheets had 2.39 kg cm − 2 , 25.3 kgf and 11 kgf bursting strength, strip strength and stiffness, respectively. The results demonstrated that the bio-sheets under optimal conditions possessed sufficient physical qualities. These characteristics imply that the bio-sheets can also be improved to create bio-bags. Using ecologically friendly materials, specifically tapioca starch and sugarcane bagasse fibre (SBF), Asrofi et al. ( 2020 ) attempted to develop composite bioplastic. SBF is added to the tapioca matrix to reinforce the structural integrity of composite bioplastics. The findings demonstrate that adding ultrasonication improves the tensile strength of the composite bioplastic samples. The sample that underwent 15 minutes of ultrasonication at 2.5 MPa had the highest tensile strength. Ali et al. ( 2022 ) created a PVA/starch nanocomposite film reinforced with sugarcane bagasse cellulose nanofiber (CNF) as an alternative to the current biodegradable plastic packaging options. Using ultrasonication and alkaline and mild acid treatment, cellulose nanofibers (CNF) were separated from sugarcane bagasse (SCB). It enhanced antimicrobial, thermal, and mechanical qualities. Using an N-dimethylacetamide/lithium chloride solvent, sugarcane bagasse nanofibers were converted into an all-cellulose nanocomposite (ACNC) film. The nanofiber sheet, ACNC and fibre sheet produced with a 10-minute dissolution time had respective tensile strengths of 8 MPa, 101 MPa, and 140 MPa. As the dissolution period increased, the ACNC film's water vapour permeability (WVP) rose proportionately. Because of its promising qualities, ACNC may be used in cellulose-based food packaging (Ghadheri et al. 2014). In 2020, Azmin et al. used sugarcane bagasse and cocoa pod husk to create biodegradable plastic films. Future research should concentrate on the environmentally friendly conversion of cellulose and its functional derivatives with increased efficiency and high selectivity. Conclusion Sugarcane bagasse exhibited great potential for cellulose extraction. The process conditions were optimised to maximise the cellulose yield. The process conditions with 5% NaOH and 1.5% NaOCl were the optimum, with the highest cellulose yield of 56.5 ± 0.2%. FTIR analysis revealed the removal of lignin and hemicellulose from sugarcane bagasse after treatment, as the peaks at 1723 cm⁻¹, 1511 cm⁻¹ and 1292 cm⁻¹ responsible for these components were missing in the extracted cellulose samples. However, the peaks relating to C–O–C and O–H confirmed the presence of cellulose. The XRD results showed an enhancement in cellulose crystallinity following alkali pretreatment. FE-SEM imaging effectively highlighted the structural transformations caused by the treatment, and an increase in surface porosity and accessibility was observed. Thus, it can be concluded that cellulose was successfully isolated from SCB. Statistical analysis also revealed a significant difference (p ≤ 0.05) in the yield of cellulose after applying the varying concentrations of NaOH and NaOCl as compared to raw SCB. The cellulose generated can find application in biofilm preparation after adding suitable binders. While the current study did not examine the effects of time and temperature, future investigations should explore how changes in these pre-treatment conditions influence the cellulose extraction to achieve a deeper understanding. Further evaluation of the cost-efficiency of these processes will provide critical insights for their adoption in sustainable industrial practices. Declarations Conflict of Interest The authors declare that they have no competing interests. Funding No funding was received for the preparation of this manuscript. Author Contribution This study was a collaborative effort, with contributions from all authors. Shikha Kumari conceptualized and designed the study, performed the experiments, and prepared the initial draft of the manuscript. Dinesh Arora conducted the statistical analyses and gave valuable sugestions for the improvement of manuscript. Dr. Manjeet Kaur and Dr. Geeta Dhania thoroughly reviewed the manuscript, offered valuable insights, and finalized the paper for submission. All authors read and approved the final version of the manuscript. Acknowledgement The author gratefully acknowledges the support and encouragement provided by the Department of Environmental Science, Maharshi Dayanand University, Rohtak, Haryana, India throughout the course of this research. The facilities, academic environment, and valuable guidance offered by the department played a significant role in the successful completion of this study. Data Availability Statement This study did not generate or analyze any datasets. References Abdel-Halim ES (2014) Chemical modification of cellulose extracted from sugarcane bagasse: Preparation of hydroxyethyl cellulose. Arabian J Chem 7(3). https://doi.org/10.1016/j.arabjc.2013.05.006 Ali MASS, Jimat DN, Nawawi WMFW, Sulaiman S (2022) Antibacterial, mechanical and thermal properties of PVA/starch composite film reinforced with cellulose nanofiber of sugarcane bagasse. Arabian J Sci Eng 47(5). https://doi.org/10.1007/s13369-021-05336-w Alirach V, Lubwama M, Olupot PW, Kukunda L (2023) Development and characterisation of bio-sheets from sugarcane bagasse as a potential packaging material. Biomass Convers Biorefinery. https://doi.org/10.1007/s13399-023-04689-6 Alokika A, Anu Kumar A, Kumar V, Singh B (2021) Cellulosic and hemicellulosic fractions of sugarcane bagasse: Potential, challenges and future perspective. Int J Biol Macromol 169. https://doi.org/10.1016/j.ijbiomac.2020.12.175 Ashokkumar V, Rajendran S, Balakrishnan M, Singh S, Pradeep Kumar R, Jayaraman R (2022) Recent advances in lignocellulosic biomass for biofuels and value-added bioproducts – a critical review. Bioresour Technol 344:126195. https://doi.org/10.1016/j.biortech.2021.126195 Asrofi M, Sapuan SM, Ilyas RA, Ramesh M (2020) Characteristic of composite bioplastics from tapioca starch and sugarcane bagasse fiber: Effect of time duration of ultrasonication (Bath-Type). Mater Today: Proc 46. https://doi.org/10.1016/j.matpr.2020.07.254 Aziz T, Farid A, Haq F, Kiran M, Ullah A, Zhang K, Li C, Ghazanfar S, Sun H, Ullah R, Ali A, Muzammal M, Shah M, Akhtar N, Selim S, Hagagy N, Samy M, al Jaouni SK (2022) A Review on the Modification of Cellulose and Its Applications. Polymers 14(15). https://doi.org/10.3390/polym14153206 Azmin SNHM, Hayat NABM, Nor MSM (2020) Development and characterization of food packaging bioplastic film from cocoa pod husk cellulose incorporated with sugarcane bagasse fibre. J Bioresour Bioproducts 5(4). https://doi.org/10.1016/j.jobab.2020.10.003 Balasubramani V, Nagarajan KJ, Karthic M, Pandiyarajan R (2024) Extraction of lignocellulosic fiber and cellulose microfibrils from agro waste-palmyra fruit peduncle: Water retting, chlorine-free chemical treatments, physio-chemical, morphological, and thermal characterization. Int J Biol Macromol 259. https://doi.org/10.1016/j.ijbiomac.2024.129273 Bangar SP, Whiteside WS, Kajla P, Tavassoli M (2023) Value addition of rice straw cellulose fibers as a reinforcer in packaging applications. Int J Biol Macromol 243. https://doi.org/10.1016/j.ijbiomac.2023.125320 Ben F, Olubambi PA (2024) Investigating the tribological behavior of bioinspired surfaces in agro-waste and alumina reinforced AA6063 matrix composites. Vacuum 230:113687. https://doi.org/10.1016/j.vacuum.2024.113687 Bledzki AK, Mamun AA, Volk J (2010) Physical, chemical and surface properties of wheat husk, rye husk and soft wood and their polypropylene composites. Compos Part A Appl Sci Manuf 41(4):480–488 Cao Y, Shibata S, Fukumoto I (2006) Mechanical properties of biodegradable composites reinforced with bagasse fibre before and after alkali treatments. Composites Part A: Appl Sci Manuf 37(3). https://doi.org/10.1016/j.compositesa.2005.05.045 Debnath B, Haldar D, Purkait MK (2021) A critical review on the techniques used for the synthesis and applications of crystalline cellulose derived from agricultural wastes and forest residues. Carbohydr Polym 273. https://doi.org/10.1016/j.carbpol.2021.118537 del Sole R, Mele G, Bloise E, Mergola L (2021) Green aspects in molecularly imprinted polymers by biomass waste utilization. Polymers 13(15). https://doi.org/10.3390/polym13152430 Ding K, Yao Z, Yimamu N, Aydan T, Guan Q (2025) Optimization and characterization of cellulose extraction from cotton straw using alkali pretreatment. Biomass Convers Biorefinery 1–14. https://doi.org/10.1007/s13399-025-05036-6 Feleke K, Thothadri G, Beri Tufa H, Rajhi AA, Ahmed GMS (2023) Extraction and Characterization of Fiber and Cellulose from Ethiopian Linseed Straw: Determination of Retting Period and Optimization of Multi-Step Alkaline Peroxide Process. Polymers 15(2). https://doi.org/10.3390/polym15020469 Freitas PAV, González-Martínez C, Chiralt A (2022) Applying ultrasound-assisted processing to obtain cellulose fibres from rice straw to be used as reinforcing agents. Innov Food Sci Emerg Technol 76. https://doi.org/10.1016/j.ifset.2022.102932 Freitas PAV, Santana LG, González-Martínez C, Chiralt A (2024) Combining subcritical water extraction and bleaching with hydrogen peroxide to obtain cellulose fibres from rice straw. Carbohydr Polym Technol Appl 7. https://doi.org/10.1016/j.carpta.2024.100491 Galiwango E, Abdel Rahman NS, Al-Marzouqi AH, Abu-Omar MM, Khaleel AA (2019) Isolation and characterization of cellulose and α-cellulose from date palm biomass waste. Heliyon 5(12). https://doi.org/10.1016/j.heliyon.2019.e02937 Gangil S, Wakudkar HM (2013) Generation of bio-char from crop residues. Int J Emerg Technol Adv Eng 3:566–570 Ghaderi M, Mousavi M, Yousefi H, Labbafi M (2014) All-cellulose nanocomposite film made from bagasse cellulose nanofibers for food packaging application. Carbohydr Polym 104(1). https://doi.org/10.1016/j.carbpol.2014.01.013 Guilherme AA, Dantas PVF, Santos ES, Fernandes FAN, Macedo GR (2015) Evaluation of composition, characterization and enzymatic hydrolysis of pretreated sugar cane bagasse. Braz J Chem Eng 32(1). https://doi.org/10.1590/0104-6632.20150321s00003146 Han W, Geng Y (2023) Optimization and characterization of cellulose extraction from olive pomace. Cellulose 30(8). https://doi.org/10.1007/s10570-023-05195-8 Hashim MY, Amin AM, Marwah OMF, Othman MH, Yunus MR, Chuan Huat N (2017) The effect of alkali treatment under various conditions on physical properties of kenaf fiber. J Phys Conf Ser 914(1). https://doi.org/10.1088/1742-6596/914/1/012030 Jung W, Savithri D, Sharma-Shivappa R, Kolar P (2020) Effect of Sodium Hydroxide Pretreatment on Lignin Monomeric Components of Miscanthus × giganteus and Enzymatic Hydrolysis. Waste Biomass Valorization 11(11). https://doi.org/10.1007/s12649-019-00859-8 Kadla JF, Gilbert RD (2000) Cellulose structure: A review. Cellul Chem Technol 34(3–4):197–216 Kapdi D, Bhavsar N, Rudakiya D (2024) Developing sustainable and energy efficient process to obtain high purity cellulose and its value-added chemicals from rice straw. Bioresour Technol Rep 25. https://doi.org/10.1016/j.biteb.2023.101732 Katakojwala R, Mohan SV (2020) Microcrystalline cellulose production from sugarcane bagasse: Sustainable process development and life cycle assessment. J Clean Prod 249. https://doi.org/10.1016/j.jclepro.2019.119342 Kathirselvam M, Kumaravel A, Arthanarieswaran VP, Saravanakumar SS (2019) Characterization of cellulose fibers in Thespesia populnea barks: Influence of alkali treatment. Carbohydr Polym 217:178–189. https://doi.org/10.1016/j.carbpol.2019.04.038 Khalid AM, Hossain MS, Ismail N et al (2021) Isolation and characterization of magnetic oil palm empty fruits bunch cellulose nanofiber composite as a bio-sorbent for Cu(II) and Cr(VI) removal. Polymers (Basel) 13:1–22. https://doi.org/10.3390/polym13010112 Khiewsawai N, Rattanawongwiboon T, Ummartyotin S (2024) Cellulose fiber derived from sugarcane bagasse and polyethylene glycol/acrylic acid/branched polyethylenimine-based hydrogel composite prepared by gamma irradiation: A platform for mercury (II) ions adsorption. Environ Adv 17:100561. https://doi.org/10.1016/j.envadv.2024.100561 Khui PLN, Rahman MR, Bakri MKB (2021) A review on the extraction of cellulose and nanocellulose as a filler through solid waste management. J Thermoplast Compos Mater. https://doi.org/10.1177/08927057211020800 Kininge MM, Gogate PR (2022) Intensification of alkaline delignification of sugarcane bagasse using ultrasound assisted approach. Ultrason Sonochem 82:105870. https://doi.org/10.1016/j.ultsonch.2021.105870 Kumar A, Ewing AH, Wereley ST (2014) Optical tweezers for manipulating cells and particles. In: Encyclopedia of Microfluidics and Nanofluidics (pp 1–8). Springer US. https://doi.org/10.1007/978-3-642-27758-0_1162-2 Kundu S, Mitra D, Das M (2023) Influence of chlorite treatment on the fine structure of alkali pretreated sugarcane bagasse. Biomass Convers Biorefinery 13(2). https://doi.org/10.1007/s13399-020-01120-2 Laluce C, Roldan IU, Pecoraro E, Igbojionu LI, Ribeiro CA (2019) Effects of pretreatment applied to sugarcane bagasse on composition and morphology of cellulosic fractions. Biomass Bioenergy 126:231–238. https://doi.org/10.1016/j.biombioe.2019.05.015 Lamo C, Bargale PC, Gangil S, Chakraborty S, Tripathi MK, Kotwaliwale N, Modhera B (2024) High crystalline cellulose extracted from chickpea husk using alkali treatment. Biomass Convers Biorefinery 14(1). https://doi.org/10.1007/s13399-022-02331-5 Liu X, Jiang Y, Song X, Qin C, Wang S, Li K (2019) A bio-mechanical process for cellulose nanofiber production – Towards a greener and energy conservation solution. Carbohydr Polym 208. https://doi.org/10.1016/j.carbpol.2018.12.071 Loh YR, Sujan D, Rahman ME, Das CA (2013) Review Sugarcane bagasse - The future composite material: A literature review. Resour Conserv Recycl 75. https://doi.org/10.1016/j.resconrec.2013.03.002 Lou C, Zhou Y, Yan A, Liu Y (2022) Extraction cellulose from corn-stalk taking advantage of pretreatment technology with immobilized enzyme. RSC Adv 12(2). https://doi.org/10.1039/d1ra07513f Lu P, Hsieh Ylo (2012) Cellulose isolation and core-shell nanostructures of cellulose nanocrystals from chardonnay grape skins. Carbohydr Polym 87(4). https://doi.org/10.1016/j.carbpol.2011.11.023 Magalhães S, Fernandes C, Pedrosa JFS, Alves L, Medronho B, Ferreira PJT, Rasteiro MdG (2023) Eco-friendly methods for extraction and modification of cellulose: An overview. Polymers 15(14). https://doi.org/10.3390/polym15143138 Mahmud MA, Anannya FR (2021) Sugarcane bagasse - A source of cellulosic fiber for diverse applications. Heliyon 7(8). https://doi.org/10.1016/j.heliyon.2021.e07771 Martin-Bertelsen B, Andersson E, Köhnke T, Hedlund A, Stigsson L, Olsson U (2020) Revisiting the dissolution of cellulose in NaOH as “Seen” by X-rays. Polymers 12(2). https://doi.org/10.3390/polym12020342 Melesse GT, Hone FG, Mekonnen MA (2022) Extraction of cellulose from sugarcane bagasse optimization and characterization. Adv Mater Sci Eng 2022. https://doi.org/10.1155/2022/1712207 Mohammed M, Oleiwi JK, Mohammed AM, Jawad AJafar M, Osman AF, Adam T, Betar BO, Gopinath SCB, Dahham OS, Jaafar M (2023) Comprehensive insights on mechanical attributes of natural-synthetic fibres in polymer composites. J Mater Res Technol 25. https://doi.org/10.1016/j.jmrt.2023.06.148 Moubarik A, Grimi N, Boussetta N (2013) Structural and thermal characterization of Moroccan sugar cane bagasse cellulose fibers and their applications as a reinforcing agent in low density polyethylene. Compos Part B Eng 52:233–238. https://doi.org/10.1016/j.compositesb.2013.04.012 Mubarak AA, Ilyas RA, Ngadi N, Nordin AH, Alkbir FM (2024) Isolation and characterization of cellulose from sugarcane bagasse fiber ( Saccharum officinarum ) via delignification and mercerization treatment using response surface modeling (RSM). Biomass Convers Biorefinery. https://doi.org/10.1007/s13399-024-05692-1 Nandiyanto ABD, Oktiani R, Ragadhita R (2019) How to read and interpret FTIR spectroscope of organic material. Indones J Sci Technol 4(1). https://doi.org/10.17509/ijost.v4i1.15806 Perumal AB, Sellamuthu PS, Nambiar RB, Sadiku ER (2018) Development of polyvinyl alcohol/chitosan bio-nanocomposite films reinforced with cellulose nanocrystals isolated from rice straw. Appl Surf Sci 449. https://doi.org/10.1016/j.apsusc.2018.01.022 Phiri R, Rangappa SM, Andoko A, Gapsari F, Siengchin S (2024) Modification of cellulose in sugarcane bagasse fibers towards development of biocomposite. Biomass Convers Biorefinery 1–17. https://doi.org/10.1007/s13399-024-05702-9 Rahayu A, Hanum FF, Amrillah NAZ, Lim LW, Salamah S (2022) Cellulose extraction from coconut coir with alkaline delignification process. J Fibers Polym Compos 1(2). https://doi.org/10.55043/jfpc.v1i2.51 Rana V, Malik S, Joshi G, Rajput NK, Gupta PK (2021) Preparation of alpha cellulose from sugarcane bagasse and its cationization: Synthesis, characterization, validation and application as wet-end additive. Int J Biol Macromol 170. https://doi.org/10.1016/j.ijbiomac.2020.12.165 Rasheed HA, Adeleke AA, Nzerem P, Olosho AI, Ogedengbe TS, Jesuloluwa S (2024) Isolation, characterization and response surface method optimization of cellulose from hybridized agricultural wastes. Sci Rep 14(1). https://doi.org/10.1038/s41598-024-65229-4 Razali NAM, Sohaimi RM, Othman RNIR, Abdullah N, Demon SZN, Jasmani L, Yunus WMZW, Ya’acob WMHW, Salleh EM, Norizan MN, Halim NA (2022) Comparative study on extraction of cellulose fiber from rice straw waste from chemo-mechanical and pulping method. Polymers 14(3). https://doi.org/10.3390/polym14030387 Rizwan M, Gilani SR, Durrani AI, Naseem S (2021) Cellulose extraction of Alstonia scholaris : A comparative study on efficiency of different bleaching reagents for its isolation and characterization. Int J Biol Macromol 191. https://doi.org/10.1016/j.ijbiomac.2021.09.155 Saad AA, Ahmed HS, Megally IAN, Ahmed M, Ibraheem MT, Farouk SM, Khalaf ESA (2022) Optimization and characterization of cellulose extracted from sugarcane bagasse. In: The International Undergraduate Research Conference, Vol. 6(6), pp 1–9. The Military Technical College Sant’Ana da Silva A, Lee SH, Endo T, Bon EPS (2011) Major improvement in the rate and yield of enzymatic saccharification of sugarcane bagasse via pretreatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate ([Emim][Ac]). Bioresour Technol 102(22). https://doi.org/10.1016/j.biortech.2011.08.085 Sayakulu NF, Soloi S (2022) The effect of sodium hydroxide (NaOH) concentration on oil palm empty fruit bunch (OPEFB) cellulose yield. J Phys Conf Ser 2314(1). https://doi.org/10.1088/1742-6596/2314/1/012017 Sayed E, Khalaf A (2023) Optimization and characterization of cellulose extracted from sugarcane bagasse. Res Sq 1–15. https://doi.org/10.21203/rs.3.rs-2618002/v1 Shaikh HM, Pandare KV, Nair G, Varma AJ (2009) Utilization of sugarcane bagasse cellulose for producing cellulose acetates: Novel use of residual hemicellulose as plasticizer. Carb Polym. 2;76(1):23-9. Sharma V, Nargotra P, Sharma S, Sawhney D, Vaid S, Bangotra R, Dutt HC, Bajaj BK (2023) Microwave irradiation-assisted ionic liquid or deep eutectic solvent pretreatment for effective bioconversion of sugarcane bagasse to bioethanol. Energy Ecol Environ 8(2). https://doi.org/10.1007/s40974-022-00267-0 Song K, Zhu X, Zhu W, Li X (2019) Preparation and characterization of cellulose nanocrystal extracted from Calotropis procera biomass. Bioresour Bioprocess 6(1). https://doi.org/10.1186/s40643-019-0279-z Stevenson L, Phillips F, O’Sullivan K, Walton J (2012) Wheat bran: Its composition and benefits to health, a European perspective. Int J Food Sci Nutr 63(8). https://doi.org/10.3109/09637486.2012.687366 Sun JX, Sun XF, Zhao H, Sun RC (2004) Isolation and characterization of cellulose from sugarcane bagasse. Polym Degrad Stab 84(2):331–339. https://doi.org/10.1016/j.polymdegradstab.2004.02.008 Sun S, Sun S, Cao X, Sun R (2016) The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials. Bioresour Technol 199. https://doi.org/10.1016/j.biortech.2015.08.061 TAPPI T 222 om-02 (2002) Acid-insoluble lignin in wood and pulp. TAPPI Test Methods. TAPPI T204 cm-17 (2017) Solvent Extractives of Wood and Pulp. TAPPI Test Methods. Ungprasoot P, Muanruksa P, Tanamool V, Winterburn J, Kaewkannetra P (2021) Valorization of aquatic weed and agricultural residues for innovative biopolymer production and their biodegradation. Polymers 13(17):2838. https://doi.org/10.3390/polym13172838 Uzoh Raymond D, Jildawa D, Sudi P (2024) Extraction and characterization of cellulose from agricultural biomass (sugarcane bagasse). Int J Model Appl Sci Res 3(9). https://cambridgeresearchpub.com/ijmasr/article/view/194 Velmurugan T, Suganya Priyadharshini G, Suyambulingam I, Siengchin S (2024) Extraction and characterization of Thespesia populnea leaf cellulose: a biomass to biomaterial conversion. Biomass Conversion and Biorefinery (published online: 7 August 2024). Springer-Verlag GmbH Germany, part of Springer Nature. https://doi.org/10.1007/s13399-024-05705-6 Verma D, Goh KL (2021) Effect of mercerization/alkali surface treatment of natural fibres and their utilization in polymer composites: mechanical and morphological studies. J Compos Sci 5:175. https://doi.org/10.3390/jcs5070175 Viera RGP, Filho GR, de Assunção RMN, Carla Cd, Vieira JG, de Oliveira GS (2007) Synthesis and characterization of methylcellulose from sugar cane bagasse cellulose. Carbohydr Polym 67(2). https://doi.org/10.1016/j.carbpol.2006.05.007 Wang W, Wang X, Zhang Y, Yu Q, Tan X, Zhuang X, Yuan Z (2020) Effect of sodium hydroxide pretreatment on physicochemical changes and enzymatic hydrolysis of herbaceous and woody lignocelluloses. Ind Crops Prod 145. https://doi.org/10.1016/j.indcrop.2020.112145 Wang X, Yang G, Feng Y, Ren G, Han X (2012) Optimizing feeding composition and carbon-nitrogen ratios for improved methane yield during anaerobic co-digestion of dairy, chicken manure and wheat straw. Bioresour Technol 120. https://doi.org/10.1016/j.biortech.2012.06.058 Wang Z, Qiao X, Sun K (2018) Rice straw cellulose nanofibrils reinforced poly(vinyl alcohol) composite films. Carbohydr Polym 197. https://doi.org/10.1016/j.carbpol.2018.06.025 Wibowo A, Alandro D, Killian MS, Nugroho G, Raghu SNV, Muflikhun AM (2024) Mechanical evaluation and characterization of hybrid sugarcane bagasse microfibrillated cellulose with added filler materials for use as disposable utensils. Adv Compos Mater 33(1). https://doi.org/10.1080/09243046.2023.2180793 Wise LE, Murphy M, D’Addieco AA (1946) Chlorite holocellulose: Its fractionation and bearing on summative wood analysis and on studies on the hemicellulose. Paper Trade J 122:35–43. Yadav A, Rani P, Yadav DK, Bhardwaj N, Gupta A, Bishnoi NR (2024) Enhancing enzymatic hydrolysis and delignification of sugarcane bagasse using different concentrations of sodium alkaline pretreatment. Nat Environ Pollut Technol 23(1):427–434. https://doi.org/10.46488/NEPT.2024.v23i01.037 Zhao T, Chen Z, Lin X, Ren Z, Li B, Zhang Y (2018) Preparation and characterization of microcrystalline cellulose (MCC) from tea waste. Carbohydr Polym 184. https://doi.org/10.1016/j.carbpol.2017.12.024 Supplementary Files Graphicalabstract.png Graphical abstract Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Reconsider pending major revisions 01 Mar, 2026 Reviewers agreed at journal 12 Oct, 2025 Reviewers invited by journal 28 Sep, 2025 Editor invited by journal 30 Aug, 2025 Editor assigned by journal 26 Aug, 2025 First submitted to journal 12 Aug, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7360666","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":521793472,"identity":"e1317682-4253-49b6-8528-1e681dedd838","order_by":0,"name":"shikha 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2","display":"","copyAsset":false,"role":"figure","size":458664,"visible":true,"origin":"","legend":"\u003cp\u003eProcess diagram for compositional analysis of Sugarcane bagasse.\u003c/p\u003e","description":"","filename":"floatimage3.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7360666/v1/0c4a332ed9bff5cd8186555e.jpeg"},{"id":93141967,"identity":"6267f6cf-c7ad-436c-b572-2d6bc6046f7e","added_by":"auto","created_at":"2025-10-09 13:09:15","extension":"jpeg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":798496,"visible":true,"origin":"","legend":"\u003cp\u003eIllustration of the cellulose extraction process from sugarcane bagasse.\u003c/p\u003e","description":"","filename":"floatimage4.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7360666/v1/00f448d201cb3c08b6ee418e.jpeg"},{"id":93141948,"identity":"2454ae8c-1b27-4c49-80f2-97e856807bdf","added_by":"auto","created_at":"2025-10-09 13:09:11","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":111334,"visible":true,"origin":"","legend":"\u003cp\u003eChemical composition of Sugarcane bagasse.\u003c/p\u003e","description":"","filename":"floatimage5.png","url":"https://assets-eu.researchsquare.com/files/rs-7360666/v1/fddd4bdac498cf062255abf4.png"},{"id":93141949,"identity":"d6e30192-d240-4dc7-978d-8d523b408481","added_by":"auto","created_at":"2025-10-09 13:09:11","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":1166319,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003ePictorial representation of various extracted cellulose samples.\u003c/strong\u003e\u003c/p\u003e","description":"","filename":"floatimage6.png","url":"https://assets-eu.researchsquare.com/files/rs-7360666/v1/a1449582c56890ee46d3c6e5.png"},{"id":93143051,"identity":"646b9dcd-c898-4899-b1b5-33a6978970c1","added_by":"auto","created_at":"2025-10-09 13:17:14","extension":"png","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":49830,"visible":true,"origin":"","legend":"\u003cp\u003eYield of extracted cellulose from sugarcane bagasse (different letters represent significant differences (p≤0.5).\u003c/p\u003e","description":"","filename":"floatimage7.png","url":"https://assets-eu.researchsquare.com/files/rs-7360666/v1/6320b3bab4858fa2344d472b.png"},{"id":93141946,"identity":"2321e994-2078-44cc-8ede-bfff641c74ea","added_by":"auto","created_at":"2025-10-09 13:09:10","extension":"png","order_by":7,"title":"Figure 7","display":"","copyAsset":false,"role":"figure","size":271719,"visible":true,"origin":"","legend":"\u003cp\u003eFTIR spectra of raw sugarcane bagasse (SCB) and various extracted cellulose samples.\u003c/p\u003e","description":"","filename":"floatimage8.png","url":"https://assets-eu.researchsquare.com/files/rs-7360666/v1/7582a0100684703e15ce2ded.png"},{"id":93141961,"identity":"af68b78c-ef8e-4f28-a77d-a9c7e6685aea","added_by":"auto","created_at":"2025-10-09 13:09:14","extension":"jpeg","order_by":8,"title":"Figure 8","display":"","copyAsset":false,"role":"figure","size":101828,"visible":true,"origin":"","legend":"\u003cp\u003eX-ray Diffraction Profile of SCB and Extracted cellulose SCBC3.\u003c/p\u003e","description":"","filename":"floatimage9.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7360666/v1/4e4336a216e66c8c60361038.jpeg"},{"id":93141956,"identity":"c9b1136c-9c01-4203-94ef-eba0b74e9f8b","added_by":"auto","created_at":"2025-10-09 13:09:14","extension":"jpeg","order_by":9,"title":"Figure 9","display":"","copyAsset":false,"role":"figure","size":411207,"visible":true,"origin":"","legend":"\u003cp\u003eFE-SEM Micrograph Showing Surface Morphology of the (a) SCB and (b) SCBC3.\u003c/p\u003e","description":"","filename":"floatimage10.jpeg","url":"https://assets-eu.researchsquare.com/files/rs-7360666/v1/ce9fdec8bd9eb1b80b936b8f.jpeg"},{"id":93141959,"identity":"cb233cec-0693-4f5d-8141-49bb81c3bdba","added_by":"auto","created_at":"2025-10-09 13:09:14","extension":"png","order_by":10,"title":"Figure 10","display":"","copyAsset":false,"role":"figure","size":109079,"visible":true,"origin":"","legend":"\u003cp\u003eVarious applications of cellulose extracted from lignocellulosic biomass.\u003c/p\u003e","description":"","filename":"floatimage11.png","url":"https://assets-eu.researchsquare.com/files/rs-7360666/v1/d3d09df1ac63f6fe97912094.png"},{"id":93143070,"identity":"7df2edbb-6866-4ed5-aa27-40aab46ddd9f","added_by":"auto","created_at":"2025-10-09 13:17:22","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":4757770,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7360666/v1/18272fc6-be17-48cc-80d0-8a85a1323f1d.pdf"},{"id":93141971,"identity":"0653cdd0-56bb-4f37-afa6-6e7d240e3514","added_by":"auto","created_at":"2025-10-09 13:09:16","extension":"png","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":564564,"visible":true,"origin":"","legend":"\u003cp\u003eGraphical abstract\u003c/p\u003e","description":"","filename":"Graphicalabstract.png","url":"https://assets-eu.researchsquare.com/files/rs-7360666/v1/4cd54c5097e1b95b7bc68bd5.png"}],"financialInterests":"","formattedTitle":"Enhanced Cellulose Isolation from Sugarcane Bagasse through Sequential Alkali and Oxidative Treatment","fulltext":[{"header":"Introduction","content":"\u003cp\u003eThe rapid generation of agricultural waste has become a growing environmental concern. It often leads to open-field burning as a method of disposal and this practice significantly contributes to greenhouse gas emissions and air pollution, exacerbating environmental and health issues (Ben et al. 2024; Debnath et al. \u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e2021\u003c/span\u003e). This agricultural waste is an eco-friendly renewable resource and a natural carbon source; it is especially well-suited to be utilised as a precursor to create novel carbon-based materials (Sole et al. \u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e2021\u003c/span\u003e). Despite its potential, less than 5% of LCB is currently utilized for productive purposes, with the rest remaining underexploited or discarded (Ashok et al. 2022). Effective utilization of agro-waste plays a vital role in reducing environmental harm while promoting the development of sustainable materials. In particular, the valorization of lignocellulosic biomass (LCB) offers a promising solution by transforming abundant agricultural residues into value-added products. This approach not only addresses the issue of waste management but also helps mitigate environmental pollution and decrease dependence on fossil fuel-based resources (Gangil \u0026amp; Wakudkar, \u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e2013\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eBy harnessing LCB for the production of eco-friendly materials, significant progress can be made toward achieving environmental sustainability and advancing a circular bioeconomy. Lignocellulosic biomass (LCB), composed mainly of cellulose, hemicellulose, and lignin, can be sourced from sugarcane bagasse, wood chips, sawdust, rice husks, cotton linens, etc., highlighting its vast availability in nature (Khui et al. \u003cspan citationid=\"CR33\" class=\"CitationRef\"\u003e2021\u003c/span\u003e). Among the components of LCB, cellulose stands out due to its biodegradability, renewability, and its ability to be engineered into micro- and nanoscale particles that exhibit novel physicochemical properties (Shao et al. 2020). The growing interest in cellulose is due to its potential applications in biodegradable plastics, textile industry, biomedical devices, drug delivery, and landscape mulch films (Raymond et al. 2024). Harnessing cellulose from agricultural waste such as sugarcane bagasse offers a dual benefit\u0026mdash;efficient waste management and the development of eco-friendly materials for a greener future.\u003c/p\u003e\u003cp\u003eWaste products from agriculture, such as can be used as cheap, sustainable, and renewable raw materials to make cellulose and its derivatives for the Vast amounts of sugarcane bagasse waste are produced annually by processing sugarcane to make juice and alcohol (Saad et al. \u003cspan citationid=\"CR58\" class=\"CitationRef\"\u003e2022\u003c/span\u003e). The accumulation of massive quantities of sugarcane bagasse poses a complex waste problem because, up until now, it scarcely found any commercial utilisation (Shaikh et al. \u003cspan citationid=\"CR62\" class=\"CitationRef\"\u003e2009\u003c/span\u003e). Managing agricultural biomass has been regarded as an essential tactic in managing and using natural resources and maintaining environmental quality (Raymond et al. 2024). Nowadays, the sugar industry uses the majority of sugarcane bagasse as boiler fuel, feedstock, and manure. Nevertheless, the residual bagasse threatens the ecosystem (Mubarak et al. \u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). SCB primarily consists of cellulose, which is manufactured in enormous quantities worldwide (Mahmud and Ananya, 2021). According to its chemical makeup, Cellulose is a natural linear polymer composed of anhydroglucose units connected through β-glycosidic bonds between the first and fourth carbon atoms (Kadla et al. 2000). Cellulose structure is shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e. It is arranged into fibrils encased in a lignin and hemicellulose matrix. Although cellulose II chains are anti-parallel, cellulose I has a parallel chain orientation. The efficient use of sugarcane bagasse (SCB) as raw material for cellulose extraction has become more prevalent in recent years (Sun et al. \u003cspan citationid=\"CR66\" class=\"CitationRef\"\u003e2004\u003c/span\u003e). Cellulose is used extensively as a diluent, thickener, cellulose ether, bioplastics, lubricant, regenerated textile fibres, film binder, and coating in the production of tablets and capsules (Wan et al. 2018; Aziz et al.2022).\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eVarious pretreatment techniques, generally divided into four primary categories: physical, chemical, physicochemical, and biological, can be used to separate cellulose (Sun et al. \u003cspan citationid=\"CR67\" class=\"CitationRef\"\u003e2016\u003c/span\u003e). Among the different pretreatment techniques, chemical procedures use chemicals to dissolve hemicelluloses and break the bond between cellulose and lignin. This strategy has several benefits, such as a simple extraction procedure, high extraction efficiency, superior thermal stability, advantageous crystallinity, simplicity in regulating reaction conditions, and cost-effectiveness (Lou et al. \u003cspan citationid=\"CR41\" class=\"CitationRef\"\u003e2022\u003c/span\u003e). Bagasse's primary benefit is that it is a waste material that yields a very cost-effective, fully or partially biodegradable product after a few pretreatments, a crucial consideration in today's market. Furthermore, with the correct procedure, the extracted fibre can exhibit reasonably acceptable mechanical properties (Loh et al. \u003cspan citationid=\"CR40\" class=\"CitationRef\"\u003e2013\u003c/span\u003e). Numerous investigations have explored the extraction of cellulose from agro residue through chemical treatments. For instance, Mubarak et al. \u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e2024\u003c/span\u003e utilised 200 ml (10\u0026ndash;30%) of NaOCL to extract cellulose from 5g Sugarcane bagasse. However, this concentration of bleaching agent seems so high for a meager portion of bagasse. In another study, cellulose extraction from SCB employed 2\u0026ndash;18% NaOH and 38% H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e (Rasheed et al. \u003cspan citationid=\"CR55\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). Moubarik et al. (\u003cspan citationid=\"CR48\" class=\"CitationRef\"\u003e2013\u003c/span\u003e) used a two-stage treatment including hot water and NaOH(15%) for cellulose extraction from SCB, yielding 42% of cellulose. No bleaching treatment was given here, and the NaOH(15%) concentration was high. Also, the obtained yield was not much higher than that of raw sugarcane bagasse. Thus, it is discernible that most of the researchers used a high concentration of NaOH and bleaching agents.\u003c/p\u003e\u003cp\u003eThe present study attempts to find the optimum conditions for cellulose extraction. Also, it focuses on the effect of different sodium hypochlorite and sodium hydroxide concentrations on the yield and properties of extracted cellulose. The extracted cellulose was characterised with the help of FTIR, XRD and SEM. Through a straightforward recycling method that relies on removing the non-cellulosic component from SCB, the current work seeks to increase the economic value of sugarcane bagasse.\u003c/p\u003e"},{"header":"Materials and Methodology","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e\u003ch2\u003eMaterials and chemical substances\u003c/h2\u003e\u003cp\u003eRaw sugarcane bagasse (SCB) was gathered from juice merchants in the Indian state of Haryana's Rohtak area. Sodium hypochlorite from Loba Chemie was used as a bleaching agent. CDH provided sodium hydroxide and hydrogen peroxide. All chemical compounds used in this experimental study were of analytical grade.\u003c/p\u003e\u003c/div\u003e\n\u003ch3\u003eRaw Material Preparation\u003c/h3\u003e\n\u003cp\u003eTo guarantee total cleanliness, the substrate was cleaned adequately with running tap water to remove dirt and then sun-dried. Following this, the substrates were ground and sieved with a sieve of size 0.2 mm (Yadav et al. \u003cspan citationid=\"CR80\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). The sieved SCB powder was stored in a ziplock container at room temperature.\u003c/p\u003e\n\u003ch3\u003eDetermination of the Chemical composition of Sugarcane bagasse\u003c/h3\u003e\n\u003cp\u003eThe composition of SCB affects the bioconversion processes. A significant proportion of sugarcane bagasse consists of cellulose, cemicellulose and lignin. Apart from these compositional ingredients, it includes other elements like extractives, moisture, nitrogen, organic carbon, ash, and trace elements (Alokika et al. \u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e2021\u003c/span\u003e). The SCB in this study was analysed for extractive, lignin, holocellulose, cellulose, and hemicellulose content. The process diagram for the compositional analysis of Sugarcane bagasse is shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\n\u003ch3\u003eExtractives\u003c/h3\u003e\n\u003cp\u003eIn the Soxhlet extractor setup, 150 ml of acetone and 2.5 g of SCB were taken. During a 4-hour run period, the residence periods for the boiling and rising stages were meticulously set to 70 and 25 minutes, respectively, on the heating mantle. The extracted sample was allowed to air dry for a few minutes at room temperature. The removed material's weight remained constant in a convection oven set at 105\u0026deg;C. The extractive content can be calculated using Eq.\u0026nbsp;(\u003cspan refid=\"Equ1\" class=\"InternalRef\"\u003e1\u003c/span\u003e) \u003cb\u003e(\u003c/b\u003eTAPPI T 204 cm-17).\u003cdiv id=\"Equ1\" class=\"Equation\"\u003e\u003cdiv format=\"TEX\" class=\"mathdisplay\" id=\"FileID_Equ1\" name=\"EquationSource\"\u003e\n$$\\:\\text{E}\\text{x}\\text{t}\\text{r}\\text{a}\\text{c}\\text{t}\\text{i}\\text{v}\\text{e}\\text{s}\\left(\\text{%}\\right)=\\frac{W1}{W}x100$$\u003c/div\u003e\u003cdiv class=\"EquationNumber\"\u003e1\u003c/div\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003eWhere W\u0026thinsp;=\u0026thinsp;Initial weight of SCB and W1\u0026thinsp;=\u0026thinsp;Final weight obtained after treatment.\u003c/p\u003e\n\u003ch3\u003eLignin\u003c/h3\u003e\n\u003cp\u003eThe Tappi T222 om-88 (Tappi test methods) determines the amount of lignin in biomass samples. 1 g of extractive-free biomass and a 72% H\u003csub\u003e2\u003c/sub\u003eSO\u003csub\u003e4\u003c/sub\u003e solution were heated for two hours at room temperature. After diluting the sample with water to bring the sulphuric acid concentration down to 3 per cent, it is boiled for an additional four hours. The lignin is then filtered after being given time to settle. The residue is rinsed with hot water until a neutral pH is achieved. The extracted lignin content can be determined using Eq.\u0026nbsp;(\u003cspan refid=\"Equ2\" class=\"InternalRef\"\u003e2\u003c/span\u003e) (Rizwan et al. \u003cspan citationid=\"CR57\" class=\"CitationRef\"\u003e2021\u003c/span\u003e).\u003cdiv id=\"Equ2\" class=\"Equation\"\u003e\u003cdiv format=\"TEX\" class=\"mathdisplay\" id=\"FileID_Equ2\" name=\"EquationSource\"\u003e\n$$\\:Lignin\\left(\\%\\right)=\\frac{W3}{W2}x100$$\u003c/div\u003e\u003cdiv class=\"EquationNumber\"\u003e2\u003c/div\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003eWhere, W2\u0026thinsp;=\u0026thinsp;Initial sample mass and W3\u0026thinsp;=\u0026thinsp;Obtained sample mass.\u003c/p\u003e\u003cdiv id=\"Sec8\" class=\"Section2\"\u003e\u003ch2\u003eHolocellulose\u003c/h2\u003e\u003cp\u003eWith a few modifications, the conventional procedure outlined by Wise et al. (\u003cspan citationid=\"CR79\" class=\"CitationRef\"\u003e1946\u003c/span\u003e) was used to determine the holocellulose content. 10 g of SCB was bleached using hydrogen peroxide buffered with sodium hydroxide solution. The mixture was heated at 70\u0026deg;C for 4 hours. Once the mixture had cooled, the residue was filtered out, cleaned with water, dried, and weighed. Hollocellulose, a blend of cellulose and hemicellulose found in residues, can be quantified using Eq.\u0026nbsp;(\u003cspan refid=\"Equ3\" class=\"InternalRef\"\u003e3\u003c/span\u003e) (Rizwan et al.2021).\u003cdiv id=\"Equ3\" class=\"Equation\"\u003e\u003cdiv format=\"TEX\" class=\"mathdisplay\" id=\"FileID_Equ3\" name=\"EquationSource\"\u003e\n$$\\:\\text{H}\\text{o}\\text{l}\\text{o}\\text{c}\\text{e}\\text{l}\\text{l}\\text{u}\\text{l}\\text{o}\\text{s}\\text{e}\\left(\\text{%}\\right)=\\frac{\\text{W}5}{\\text{W}4}\\text{x}100$$\u003c/div\u003e\u003cdiv class=\"EquationNumber\"\u003e3\u003c/div\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003eWhere W4\u0026thinsp;=\u0026thinsp;Weight of the original sample, and W5\u0026thinsp;=\u0026thinsp;Weight of the obtained holocellulose.\u003c/p\u003e\u003c/div\u003e\n\u003ch3\u003eCellulose\u003c/h3\u003e\n\u003cp\u003eFor cellulose content determination, a 1 g sample of holocellulose and 25 ml of 17.5% NaOH were combined in a flask and heated at 95\u0026deg;C for one hour. 25 millilitres of distilled water (DW) was added, and the residue was filtered. After that, 25 millilitres of a 10% acetic acid solution was added, filtered once more, and repeatedly cleaned with distilled water. The amount of cellulose residue was determined after the sample was dried for 24 hours at 40\u0026deg;C. Silva \u003cem\u003eet al\u003c/em\u003e. (2011). Cellulose content percentage is determined using Eq.\u0026nbsp;(\u003cspan refid=\"Equ4\" class=\"InternalRef\"\u003e4\u003c/span\u003e) (Song et al. \u003cspan citationid=\"CR64\" class=\"CitationRef\"\u003e2019\u003c/span\u003e).\u003cdiv id=\"Equ4\" class=\"Equation\"\u003e\u003cdiv format=\"TEX\" class=\"mathdisplay\" id=\"FileID_Equ4\" name=\"EquationSource\"\u003e\n$$\\:\\text{C}\\text{e}\\text{l}\\text{l}\\text{u}\\text{l}\\text{o}\\text{s}\\text{e}\\left(\\text{%}\\right)=\\frac{\\text{W}7}{\\text{W}6}\\text{x}100$$\u003c/div\u003e\u003cdiv class=\"EquationNumber\"\u003e4\u003c/div\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003eWhere =\u0026thinsp;Initial amount of holocellulose and W7\u0026thinsp;=\u0026thinsp;Final amount of extracted cellulose.\u003c/p\u003e\n\u003ch3\u003eHemicellulose\u003c/h3\u003e\n\u003cp\u003eThe difference between the holocellulose and cellulose concentrations was calculated to determine the hemicellulose fraction (Eq.\u0026nbsp;5) (Balasubramani et al. \u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). The percentage that remains after deducting cellulose from holocellulose is hemicellulose. This formula is frequently used to analyse the composition of plant fibres in wood science, biomass research, and the paper industry.\u003c/p\u003e\u003cp\u003eHemicellulose\u0026thinsp;=\u0026thinsp;Holocellulose\u0026thinsp;\u0026minus;\u0026thinsp;Cellulose (5)\u003c/p\u003e\u003cdiv id=\"Sec11\" class=\"Section2\"\u003e\u003ch2\u003eIsolation of Cellulose\u003c/h2\u003e\u003cp\u003eIn this study, cellulose extraction was conducted with the help of combined and modified methods of Ungprasoot et al. (\u003cspan citationid=\"CR70\" class=\"CitationRef\"\u003e2021\u003c/span\u003e), Melesse et al. (\u003cspan citationid=\"CR46\" class=\"CitationRef\"\u003e2022\u003c/span\u003e) and Khiewsawai et al. (2023). The process of cellulose extraction from sugarcane bagasse is shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003e, and it mainly involves three steps: dewaxing using ethanol, alkaline treatment using NaOH and bleaching treatment using NaOCl. The cellulose yield(%) is greatly affected by the amount of NaOH and NaOCl used. Thus, the two are used in varying concentrations, and six samples were prepared and labeled accordingly as given in Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab1\" border=\"1\"\u003e\u003ccaption language=\"En\"\u003e\u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e\u003cdiv class=\"CaptionContent\"\u003e\u003cp\u003eChemical Processing Conditions for Cellulose Extraction from Sugarcane bagasse.\u003c/p\u003e\u003c/div\u003e\u003c/caption\u003e\u003ccolgroup cols=\"3\"\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e\u003cdiv align=\"char\" char=\".\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e\u003cthead\u003e\u003ctr\u003e\u003cth align=\"left\" colname=\"c1\"\u003e\u003cp\u003eSample\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c2\"\u003e\u003cp\u003eNaOH (%)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c3\"\u003e\u003cp\u003eNaOCl (%)\u003c/p\u003e\u003c/th\u003e\u003c/tr\u003e\u003c/thead\u003e\u003ctbody\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eSCBC1\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e1.5\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eSCBC2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e3\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eSCBC3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e1.5\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eSCBC4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e3\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eSCBC5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e8\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e1.5\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003eSCBC6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"char\" char=\".\" colname=\"c2\"\u003e\u003cp\u003e8\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e3\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003c/tbody\u003e\u003c/colgroup\u003e\u003c/table\u003e\u003c/div\u003e\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec12\" class=\"Section2\"\u003e\u003ch2\u003eDewaxing\u003c/h2\u003e\u003cp\u003eDewaxing was done using a solution of ethanol and deionised water in 1:1 v/v for around four hours, then boiling the mixture for 1.5 hours. This procedure was repeated twice to remove dust, dirt, sugar extracts, and water-soluble contaminants. To remove any remaining sugar and surfactants, the mixture was rinsed with hot water first, followed by cold water. After that, it was oven-dried for a whole day.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec13\" class=\"Section2\"\u003e\u003ch2\u003eAlkaline Treatment\u003c/h2\u003e\u003cp\u003eThree varying concentrations 2%, 5%, and 8% of sodium hydroxide (NaOH) solution(200 ml ) were applied to a 10 g sample to perform the alkaline treatment. The samples were heated for two hours, stirring occasionally, to a temperature of 75\u0026deg;C. This process made the desired changes possible, guaranteeing that the sample and the alkaline solution interacted well. They were then extensively cleaned with distilled water to neutralise the solid residue that had been produced.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec14\" class=\"Section2\"\u003e\u003ch2\u003eBleaching\u003c/h2\u003e\u003cp\u003eTwo hundred millilitres of NaOCl at two differing concentrations 1.5% and 3% was used to bleach the residue left over after the alkaline treatment. For 30 minutes, residue obtained after alkali treatment was heated to 80\u0026deg;C while being constantly stirred and then to attain a neutral pH it was cleaned with distilled water.\u003c/p\u003e\u003cp\u003eThen for 16 hours, the isolated cellulose samples were oven-dried at 50\u0026deg;C. After oven drying, it was ground into a fine powder and kept in ziplock containers to retain moisture.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec15\" class=\"Section2\"\u003e\u003ch2\u003eYield\u003c/h2\u003e\u003cp\u003eCellulose yield indicates the proportion of cellulose successfully extracted, reflecting cellulose recovery efficiency. The yield was calculated as a percentage relative to the dry weight of the SCB after treatments. Accurate measurements of the initial and final weights of SCB and SCBC were used to determine the yield using Eq.\u0026nbsp;(\u003cspan refid=\"Equ5\" class=\"InternalRef\"\u003e6\u003c/span\u003e) (Mubarak et al., \u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e2024\u003c/span\u003e).\u003cdiv id=\"Equ5\" class=\"Equation\"\u003e\u003cdiv format=\"TEX\" class=\"mathdisplay\" id=\"FileID_Equ5\" name=\"EquationSource\"\u003e\n$$\\:\\text{Y}\\text{i}\\text{e}\\text{l}\\text{d}\\left(\\text{%}\\right)=\\frac{\\text{W}9}{\\text{W}8}\\text{x}100$$\u003c/div\u003e\u003cdiv class=\"EquationNumber\"\u003e6\u003c/div\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003eW8\u0026thinsp;=\u0026thinsp;Initial weight of SCB, and W9\u0026thinsp;=\u0026thinsp;Final weight of extracted cellulose.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec16\" class=\"Section2\"\u003e\u003ch2\u003eCharacterization\u003c/h2\u003e\u003cdiv id=\"Sec17\" class=\"Section3\"\u003e\u003ch2\u003eFTIR Analysis\u003c/h2\u003e\u003cp\u003eFourier transform infrared (FTIR) Spectroscopy was used to examine the functional groups. Bruker's Invenio \u0026reg; FTIR spectrometer was employed. It had a 2 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e resolution and was operated in gearbox mode. FTIR spectra were obtained over a range of wavenumbers from 4000 to 400 cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e.\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv id=\"Sec18\" class=\"Section2\"\u003e\u003ch2\u003eXRD\u003c/h2\u003e\u003cp\u003eA multifunctional, adaptable X-ray diffractometer system of model Smartlab 3kW/Rigaku was used to conduct the X-ray diffraction analysis. With a phase of 0.04 and a scanning time of five minutes, the analysis was carried out in the 2β range value scanned from 10 to 50\u0026ordm;. Segals method [(Eq.\u0026nbsp;(7)] was used to calculate crystallinity index (Segal et al. 1959).\u003c/p\u003e\u003cp\u003eCrI (%) = [(I002 - Iam) / I002] \u0026times; 100 (7)\u003c/p\u003e\u003cp\u003eWhere, I002\u0026thinsp;=\u0026thinsp;Intensity of the crystalline peak and Iam\u0026thinsp;=\u0026thinsp;Intensity of the amorphous background\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec19\" class=\"Section2\"\u003e\u003ch2\u003eFE-SEM\u003c/h2\u003e\u003cp\u003eThe micrographs were obtained using a Carl Zeiss field emission scanning electron microscope. The aluminium stubs were covered with a layer of gold after the dried, finely powdered SCB and SCBC3 samples were put on them. Better conductivity is guaranteed by this gold coating, which also improves the quality of the images acquired during the ensuing image processing procedure.\u003c/p\u003e\u003c/div\u003e\u003cdiv id=\"Sec20\" class=\"Section2\"\u003e\u003ch2\u003eStatistical analysis\u003c/h2\u003e\u003cp\u003eThe experimental work was performed in triplicates. The results are given as the mean value and its corresponding standard deviation. The mean of altered treatments, Dunnett test for control, and analysis of variance (ANOVA) were assessed using Tukey\u0026rsquo;s test at the 0.5% statistical level in the IBM SPSS Statistics 25.0. Statistically significant differences among treatments are indicated by different letters in the figures, based on the Tukey multiple comparison test (p\u0026thinsp;\u0026le;\u0026thinsp;0.05). Graphs were generated using Origin software.\u003c/p\u003e\u003c/div\u003e"},{"header":"Results and Discussion","content":"\u003cdiv id=\"Sec22\" class=\"Section2\"\u003e\u003ch2\u003eChemical Composition of Raw Sugarcane Bagasse\u003c/h2\u003e\u003cp\u003eThe raw SCB employed in this investigation contains 7.4\u0026thinsp;\u0026plusmn;\u0026thinsp;0.9% extractives, 16.3\u0026thinsp;\u0026plusmn;\u0026thinsp;0.5% lignin, 23.6\u0026thinsp;\u0026plusmn;\u0026thinsp;2.08% hemicellulose, and 42.6\u0026thinsp;\u0026plusmn;\u0026thinsp;1.5% cellulose. The chemical composition of SCB is shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003e. The sugarcane bagasse (SCB) used in the aforementioned study has a cellulose, hemicellulose, and lignin concentration that falls within the ranges described in prior investigations. The cellulose content was close to that of Yadav et al. \u003cspan citationid=\"CR80\" class=\"CitationRef\"\u003e2024\u003c/span\u003e, where the cellulose content was 43.2%. Nonetheless, they reported 16.6% and 26.5% of lignin and hemicellulose respectively.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eThe extractive and cellulose content of the present study were higher than that of the Rasheed et al. \u003cspan citationid=\"CR55\" class=\"CitationRef\"\u003e2024\u003c/span\u003e, where 6% extractives and 41% cellulose were reported. The cellulose concentration was lower than the studies of Mohammed et al. \u003cspan citationid=\"CR47\" class=\"CitationRef\"\u003e2023\u003c/span\u003e and Ungprasoot et al. 2024 where sugarcane bagasse consists of 46% and 47% cellulose, respectively. In comparison to other agroresidues, the cellulose content was higher than wheat husk (36\u0026ndash;39%) and lower than rice straw (48.5%) (Bledzki et al. 2011; Ungprasoot et al. 202). The lignin content of 23% (Lalucea et al. 2019), 21% (Rana et al. \u003cspan citationid=\"CR54\" class=\"CitationRef\"\u003e2021\u003c/span\u003e) and 38% (Mubarak et al. \u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e2024\u003c/span\u003e) were also found to be higher than in the current study, where lignin content is 16.3\u0026thinsp;\u0026plusmn;\u0026thinsp;0.5%. The hemicellulose content was also higher in the few studies, ranging from 26.5%-35.2% (Laluce et al. \u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e2019\u003c/span\u003e; Ungprasoot et al. \u003cspan citationid=\"CR70\" class=\"CitationRef\"\u003e2021\u003c/span\u003e; Yadav et al. \u003cspan citationid=\"CR80\" class=\"CitationRef\"\u003e2024\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eTable\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e shows the comparative analysis of the chemical composition of SCB in the present study, as well as the chemical composition of SCB and different agricultural residues reported in the literature. Overall, the compositional data of sugarcane bagasse (SCB) closely aligns with values reported in the literature, highlighting its potential as a promising feedstock for various bio-based applications. Its well-balanced proportions of cellulose, hemicellulose, and lignin, combined with a moderate level of extractives, make SCB highly suitable for uses such as biofuel production, paper manufacturing, and the development of composite materials.\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab2\" border=\"1\"\u003e\u003ccaption language=\"En\"\u003e\u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e\u003cdiv class=\"CaptionContent\"\u003e\u003cp\u003eChemical composition of SCB and different agricultural residues.\u003c/p\u003e\u003c/div\u003e\u003c/caption\u003e\u003ccolgroup cols=\"6\"\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e\u003cthead\u003e\u003ctr\u003e\u003cth align=\"left\" colname=\"c1\"\u003e\u003cp\u003eAgro-residue\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c2\"\u003e\u003cp\u003eCellulose (%)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c3\"\u003e\u003cp\u003eHemicellulose (%)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c4\"\u003e\u003cp\u003eLignin (%)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c5\"\u003e\u003cp\u003eExtractive (%)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c6\"\u003e\u003cp\u003eReference\u003c/p\u003e\u003c/th\u003e\u003c/tr\u003e\u003c/thead\u003e\u003ctbody\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eSCB\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e42.6\u0026thinsp;\u0026plusmn;\u0026thinsp;1.5%\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e23.6\u0026thinsp;\u0026plusmn;\u0026thinsp;2.08%\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e16.3\u0026thinsp;\u0026plusmn;\u0026thinsp;0.5%\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e7.4\u0026thinsp;\u0026plusmn;\u0026thinsp;0.9%\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003ePresent Study\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eSCB\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e46\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e28.7\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e22.6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e2.04\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003eSilva et al. (2011)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eSCB\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e40\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e35\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e23\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003eLaluce et al. (\u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e2019\u003c/span\u003e)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eSCB\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e45.6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e29.4\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e21.2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003eRana et al. (\u003cspan citationid=\"CR54\" class=\"CitationRef\"\u003e2021\u003c/span\u003e)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eSCB\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e41\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e18\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e16\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003eRasheed et al. (\u003cspan citationid=\"CR55\" class=\"CitationRef\"\u003e2024\u003c/span\u003e)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eSCB\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e43.2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e26.5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e16.6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003eYadav et al. (\u003cspan citationid=\"CR80\" class=\"CitationRef\"\u003e2024\u003c/span\u003e)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eSCB\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e47\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e20.8\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e28\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003eUngprasoot et al. (\u003cspan citationid=\"CR70\" class=\"CitationRef\"\u003e2021\u003c/span\u003e)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eSCB\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e46\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e16\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e38\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003eMubarak et al. (\u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e2024\u003c/span\u003e)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eWheat Husk\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e36\u0026ndash;39%\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e18\u0026ndash;21%\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e16%\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003eBledzki et al., (\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e2010\u003c/span\u003e)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eWheat Bran\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e24\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e32\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e46\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003eStevenson et al. (\u003cspan citationid=\"CR65\" class=\"CitationRef\"\u003e2012\u003c/span\u003e)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eWheat Straw\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e33\u0026ndash;45\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e19\u0026ndash;32\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e8\u0026ndash;16\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003eWang et al. (\u003cspan citationid=\"CR76\" class=\"CitationRef\"\u003e2012\u003c/span\u003e)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eCalotropis procera\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e64.1\u0026thinsp;\u0026plusmn;\u0026thinsp;1.6\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e19.5\u0026thinsp;\u0026plusmn;\u0026thinsp;1.2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e9.7\u0026thinsp;\u0026plusmn;\u0026thinsp;1.2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003eSong et al. (\u003cspan citationid=\"CR64\" class=\"CitationRef\"\u003e2019\u003c/span\u003e)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eRice straw\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e48.5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e35.2\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e5\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e-\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003eUngprasoot et al. (\u003cspan citationid=\"CR70\" class=\"CitationRef\"\u003e2021\u003c/span\u003e)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e\u003cb\u003eChickpea husk\u003c/b\u003e\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e52.3\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003e31.9\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c4\"\u003e\u003cp\u003e13.9\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c5\"\u003e\u003cp\u003e1.8\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c6\"\u003e\u003cp\u003eLamo et al. (\u003cspan citationid=\"CR38\" class=\"CitationRef\"\u003e2024\u003c/span\u003e)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003c/tbody\u003e\u003c/colgroup\u003e\u003c/table\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003cb\u003eImpacts of alkali and bleaching treatment on the Cellulose yield.\u003c/b\u003e\u003c/p\u003e\u003cp\u003eThis study examined the impact of varying sodium hydroxide and sodium hypochlorite concentrations on sugarcane bagasse cellulose production. It is generally recognised that hemicellulose is dissolved and removed from the biomass due to pretreatment with NaOH causing delignification, by significantly rupturing the cross-ester linkage between complex hemicellulose and lignin (Jung et al. \u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e2020\u003c/span\u003e). According to Hashim et al. (\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e2017\u003c/span\u003e), the lignin and hemicellulose dissolve in alkali solutions, forming a black liquid when the hydrogen bonds between the lignocellulosic components are broken, increasing the yield of cellulose. After alkali treatment, hemicellulose and lignin were still present in the extracted residue, and bleaching was required to eliminate them further (Geng and Han, \u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e2023\u003c/span\u003e). Oxidising chemicals, such as sodium hypochlorite bleaching, were used to remove leftover lignin and other impurities affecting cellulose appearance and quality (Kapdi et al. \u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). The hypochlorite ion in NaOCl is a potent oxidant that can dissolve the ether bond in the lignin structure and enhance the pulp's white brightness (Sayakulu and Soloi, 2021). The brightness and purity of the cellulose were further enhanced by bleaching. Alkali pretreatment caused SCB's cellulose content to rise while hemicellulose and lignin levels were reduced. The pictorial representation of the various extracted cellulose samples can be seen in Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003e.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eIt was discovered that the yields for SCBC1 and SCBC2 were 46.1\u0026thinsp;\u0026plusmn;\u0026thinsp;0.7% and 43.3\u0026thinsp;\u0026plusmn;\u0026thinsp;0.6%, respectively. These are significantly less than the yield of 57.6% obtained in the study by Yadav et al. \u003cspan citationid=\"CR80\" class=\"CitationRef\"\u003e2024\u003c/span\u003e. Hemicellulose can be partially broken down by 2% concentrations of NaOH, which results in some hemicellulose being removed from the sugarcane bagasse. Nonetheless, the pretreated biomass might still include a sizable amount of hemicellulose (Yadav et al. \u003cspan citationid=\"CR80\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). The use of NaOCl for bleaching could be the cause of the decreased concentration. It is evident from the study of Raymond et al. 2024 that a high concentration of bleaching agent reduced the yield of cellulose. In their study, using 10\u0026ndash;20% NaOCl resulted in a lower yield, i.e., 32.2\u0026ndash;40.2%. In another study 6% NaOCl alone was used for cellulose extraction, and the yield was 48% (Laluce et al. \u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e2019\u003c/span\u003e). Thus, it is clear that the NaOCl concentration also affects the cellulose yield.\u003c/p\u003e\u003cp\u003eSample SCBC 3 produced yield of 56.5\u0026thinsp;\u0026plusmn;\u0026thinsp;0.2%. The Kapdi et al. (\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e2024\u003c/span\u003e) study also demonstrated similar outcomes, with a 58% cellulose production from rice straw. The yield for SCBC 4 was 53.1\u0026thinsp;\u0026plusmn;\u0026thinsp;0.4%. The cellulose yield was reduced significantly at high concentrations of NaOH (8%). SCBC5 and SCBC6 were 43.6\u0026thinsp;\u0026plusmn;\u0026thinsp;0.8% and 42.6\u0026thinsp;\u0026plusmn;\u0026thinsp;0.6% (Lowest Yield) respectively. The results are in harmony with the results of (Rasheed et al. \u003cspan citationid=\"CR55\" class=\"CitationRef\"\u003e2024\u003c/span\u003e), where the yield using 10% NaOH and NaOCl was 44.2%. The yield percentage of extracted cellulose samples is shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003e. It was discovered that raising the sodium hydroxide concentration reduced the yield because some of the cellulose chains would break down during the treatment procedure. The cellulose molecules were freely distributed in the solvent (NaOH) because of their stable open structure. A high concentration of NaOH will break up a few crystalline regions in cellulose and cause it to dissolve more readily in the solution treatment, lowering the amount of cellulose fibre produced (Azmin et al. \u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e2020\u003c/span\u003e). A certain quantity of cellulose may dissolve in high concentrations of NaOH, lowering the percentage of cellulose (Martin-Bertelsen et al. \u003cspan citationid=\"CR45\" class=\"CitationRef\"\u003e2020\u003c/span\u003e). This investigation's findings align with those of Kathirselvam et al. (\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e2019\u003c/span\u003e), Melesse et al. (\u003cspan citationid=\"CR46\" class=\"CitationRef\"\u003e2022\u003c/span\u003e), Rahayu et al. (\u003cspan citationid=\"CR53\" class=\"CitationRef\"\u003e2022\u003c/span\u003e), and Saad et al. (\u003cspan citationid=\"CR58\" class=\"CitationRef\"\u003e2022\u003c/span\u003e), who also showed that a higher concentration of NaOH resulted in decreased cellulose yield. NaOCl's bleaching qualities enhanced the colour of the extracted samples and aided in the dissolution of any remaining lignin and hemicellulose. SCBC1 was light brown, possibly due to the low concentration of NaOCl (1.5%). SCBC2, SCBC3 and SCBC5 were found to be pale creamish yellow, whereas SCBC4 and SCBC6 were much brighter and white in appearance. Overall, Bleaching makes the finished cellulose product more aesthetically attractive and appropriate (Kapdi et al. \u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). It can be concluded that much higher and lower concentrations result in low yield and purity, respectively. Thus, the optimum concentration for cellulose extraction were 5% NaOH and 1.5% NaOCl, resulting in highest yield 56.5\u0026thinsp;\u0026plusmn;\u0026thinsp;0.2% (SCBC3). The obtained cellulose yield reflects an effective isolation procedure and aligns well with previously reported values, further supporting the potential of sugarcane bagasse in value-added bioproduct development.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cdiv id=\"Sec23\" class=\"Section3\"\u003e\u003ch2\u003eFTIR\u003c/h2\u003e\u003cp\u003eSugarcane bagasse (SCB) and extracted cellulose can be efficiently characterised using Fourier Transform Infrared (FTIR) spectroscopy. It aids in elucidating modifications to functional groups and molecular structures brought about by the pre-treatment procedure.The spectra of SCB and all extracted samples were broadly similar, with a few significant differences that indicate structural and compositional changes. Table\u0026nbsp;\u003cspan refid=\"Tab3\" class=\"InternalRef\"\u003e3\u003c/span\u003e unfolds the Functional groupspresent in the FTIR spectra of Sugarcane bagasse and Sugarcane bagasse cellulose. The broad peak at 3326 cm⁻\u0026sup1; and 3338\u0026ndash;3339 cm⁻\u0026sup1; in raw SCB and all the extracted cellulose samples, respectively, confirms the presence of hydroxyl (-OH) groups, indicating strong hydrogen bonding, a characteristic of cellulose (Nandiyanto et al. \u003cspan citationid=\"CR50\" class=\"CitationRef\"\u003e2019\u003c/span\u003e). As the purification process advanced, the bell-shaped absorption band between 2980 and 3690 cm⁻\u0026sup1; became somewhat narrower (Wang et al. \u003cspan citationid=\"CR77\" class=\"CitationRef\"\u003e2018\u003c/span\u003e). Similar peaks at approximately 3434.5 cm⁻\u0026sup1; and 3426.1 cm⁻\u0026sup1;were found for the bagasse and the extracted cellulose, respectively (Lu et al. 2012). Figure\u0026nbsp;\u003cspan refid=\"Fig7\" class=\"InternalRef\"\u003e7\u003c/span\u003e presents the FTIR spectra of Raw SCB and extracted cellulose samples. The peak at 2890\u0026ndash;2902 cm⁻\u0026sup1;in extracted cellulose samples and SCB were associated with C\u0026ndash;H stretching from polysaccharides, validating the sample's organic composition by matching the C\u0026ndash;H stretching vibrations of the methyl and methylene groups in polysaccharides (Saad et al. \u003cspan citationid=\"CR58\" class=\"CitationRef\"\u003e2022\u003c/span\u003e; Mubarak et al. \u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e2024\u003c/span\u003e).\u003c/p\u003e\u003cp\u003e\u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab3\" border=\"1\"\u003e\u003ccaption language=\"En\"\u003e\u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e\u003cdiv class=\"CaptionContent\"\u003e\u003cp\u003eFunctional groups in the FTIR spectra of Sugarcane bagasse and Sugarcane bagasse cellulose.\u003c/p\u003e\u003c/div\u003e\u003c/caption\u003e\u003ccolgroup cols=\"3\"\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e\u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e\u003cthead\u003e\u003ctr\u003e\u003cth align=\"left\" colspan=\"2\" nameend=\"c2\" namest=\"c1\"\u003e\u003cp\u003eWavenumber (cm\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e)\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c3\" morerows=\"1\" rowspan=\"2\"\u003e\u003cp\u003eFunctional group assignment\u003c/p\u003e\u003c/th\u003e\u003c/tr\u003e\u003ctr\u003e\u003cth align=\"left\" colname=\"c1\"\u003e\u003cp\u003eSugarcane bagasse\u003c/p\u003e\u003c/th\u003e\u003cth align=\"left\" colname=\"c2\"\u003e\u003cp\u003eSugarcane bagasse cellulose\u003c/p\u003e\u003c/th\u003e\u003c/tr\u003e\u003c/thead\u003e\u003ctbody\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e3326\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e3338\u0026ndash;3339\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003eO-H Stretching vibrations\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e2890\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e2890\u0026ndash;2902\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003eC-H Stretching\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e1723\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e-\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003eCarbonyl (C\u0026thinsp;=\u0026thinsp;O) stretching\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e1630\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e1612\u0026ndash;1630\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003eOH (Water Absorbed)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e1511\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e-\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003eAromatic C\u0026thinsp;=\u0026thinsp;C Stretching\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e1292\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e-\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003eAromatic C-O Stretching\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e1152\u0026thinsp;\u0026minus;\u0026thinsp;1015\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e1123\u0026ndash;1167\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003eC-O-C Stretching\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e895\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e982\u0026ndash;1018\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003eC-O Stretching Vibration (Cellulose)\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e-\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e816\u0026ndash;841\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003eRing Deformation\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003ctr\u003e\u003ctd align=\"left\" colname=\"c1\"\u003e\u003cp\u003e653\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c2\"\u003e\u003cp\u003e622\u0026thinsp;\u0026minus;\u0026thinsp;594\u003c/p\u003e\u003c/td\u003e\u003ctd align=\"left\" colname=\"c3\"\u003e\u003cp\u003eC-H Bending\u003c/p\u003e\u003c/td\u003e\u003c/tr\u003e\u003c/tbody\u003e\u003c/colgroup\u003e\u003c/table\u003e\u003c/div\u003e\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eThe H\u0026ndash;O\u0026ndash;H bending of absorbed water is responsible for the peak at 1612\u0026ndash;1630 cm⁻\u0026sup1;, indicating that the SCB and extracted cellulose contain some moisture. The moisture content of cellulose is marked by this area in other studies by Wang et al. (\u003cspan citationid=\"CR75\" class=\"CitationRef\"\u003e2020\u003c/span\u003e), Saad et al. (\u003cspan citationid=\"CR58\" class=\"CitationRef\"\u003e2022\u003c/span\u003e), Freitas et al. (\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e2022\u003c/span\u003e) and Mubarak et al. (\u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e2024\u003c/span\u003e) with peaks in the range 1629 cm⁻\u0026sup1;to 1645 cm⁻\u0026sup1;. Further Peaks in the 1123\u0026ndash;1167 cm⁻\u0026sup1;and 1018\u0026ndash;982 cm⁻\u0026sup1; ranges are present in extracted cellulose and in the range 1152 cm⁻\u0026sup1;-1015 cm⁻\u0026sup1; and at 895 cm⁻\u0026sup1; in SCB confirming C\u0026ndash;O\u0026ndash;C and C\u0026ndash;O stretching respectively, which are traits of cellulose's glycosidic bonds which are crucial cellulose structural elements. The polysaccharide structure is confirmed by the literature, which shows that absorption bands in the 1162 cm⁻\u0026sup1;-899 cm⁻\u0026sup1; range are linked to C\u0026ndash;O\u0026ndash;C and C\u0026ndash;O stretching vibrations (Sayed and Khalaf, \u003cspan citationid=\"CR61\" class=\"CitationRef\"\u003e2023\u003c/span\u003e; Yadav et al. \u003cspan citationid=\"CR80\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). SCB peaked at 653 cm⁻\u0026sup1;, corresponding to C\u0026ndash;H bending. All the extracted cellulose samples exhibited Peaks at 816\u0026ndash;841 cm⁻\u0026sup1; and 622\u0026ndash;594 cm⁻\u0026sup1;, and are associated with ring deformation and C\u0026ndash;H bending, common in cellulose structures. These results align with the literature (Mubarak et al. \u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e2024\u003c/span\u003e; Freitas et al. \u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e2024\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eRaw SCB exhibited peaks at 1723 cm⁻\u0026sup1;, 1511 cm⁻\u0026sup1; and 1292 cm⁻\u0026sup1;, which correspond to C\u0026thinsp;=\u0026thinsp;O stretching that represents aldehyde, ketone, or carboxylic acids in hemicellulose, aromatic C\u0026thinsp;=\u0026thinsp;C stretching of lignin and C-O Stretching vibrations of lignin and hemicellulose, respectively. These peaks were either absent or were reduced in the extracted cellulose samples, which indicates the removal of hemicellulose and lignin, suggesting that cellulose has been successfully separated from sugarcane bagasse. Previous studies demonstrated similar findings (Viera et al. \u003cspan citationid=\"CR74\" class=\"CitationRef\"\u003e2007\u003c/span\u003e; Zhao et al. \u003cspan citationid=\"CR81\" class=\"CitationRef\"\u003e2018\u003c/span\u003e; Kapdi et al. \u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e2024\u003c/span\u003e).Identifying the extracted sample as cellulose is strengthened by the close alignment of FTIR data with known cellulose spectrum features. While the water-related peak indicates some bound moisture, hydroxyl groups, glycosidic connections, and C\u0026ndash;O stretching validate the cellulose structure. The FTIR peaks show that lignin and hemicellulose have been significantly removed. Typically, The current samples peak at 1612\u0026ndash;1630 cm⁻\u0026sup1;, not the aromatic C\u0026thinsp;=\u0026thinsp;C stretching of lignin but rather H\u0026ndash;O\u0026ndash;H bending (absorbed water). The presence of cellulose is confirmed by the prominent peaks in your spectrum for O\u0026ndash;H stretching (3338 cm⁻\u0026sup1;), C\u0026ndash;O\u0026ndash;C glycosidic linkages (1123\u0026ndash;1167 cm⁻\u0026sup1;), and C\u0026ndash;O stretching (1018\u0026thinsp;\u0026minus;\u0026thinsp;982 cm⁻\u0026sup1;). Rather than lignin or hemicellulose, the peaks at 816\u0026ndash;841 cm\u0026sup1; and 622\u0026ndash;594 cm\u0026sup1; are characteristic of cellulose. The presence and successful isolation of cellulose are confirmed by the preserved C\u0026ndash;O\u0026ndash;C and C\u0026ndash;H peaks. Overall, the work was in harmony with (Sun et al. \u003cspan citationid=\"CR66\" class=\"CitationRef\"\u003e2004\u003c/span\u003e; Viera et al. \u003cspan citationid=\"CR74\" class=\"CitationRef\"\u003e2007\u003c/span\u003e; Perumal et al. \u003cspan citationid=\"CR51\" class=\"CitationRef\"\u003e2018\u003c/span\u003e; Laluce et al. \u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e2019\u003c/span\u003e; Katakojwala and Mohan \u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e2020\u003c/span\u003e; Rasheed et al. \u003cspan citationid=\"CR55\" class=\"CitationRef\"\u003e2024\u003c/span\u003e; Freitas et al. \u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). FTIR data indicates that the sugarcane bagasse has successfully undergone hemicellulose removal and delignification, leaving behind purified cellulose.\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv id=\"Sec24\" class=\"Section2\"\u003e\u003ch2\u003eXRD\u003c/h2\u003e\u003cp\u003eX-ray diffraction (XRD) is a crucial method for figuring out a material's crystallographic structure. This method includes subjecting a material to X-ray radiation and then determining the angles and intensities of X-ray scattering that result (Mubarak et al. \u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). Cellulose, lignin, and hemicellulose make up most of SCB; cellulose is crystalline, whereas lignin and hemicellulose are amorphous (Kundu et al. \u003cspan citationid=\"CR36\" class=\"CitationRef\"\u003e2023\u003c/span\u003e). The present study examined structural changes and assessed the impact of alkali (NaOH) and bleaching (NaOCl) on the crystallinity of the resultant cellulose using XRD analysis of raw sugarcane bagasse (SCB) and SCBC3, which yielded the maximum amount of cellulose. The XRD spectra of SCB and SCBC3 are shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig8\" class=\"InternalRef\"\u003e8\u003c/span\u003e.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eThere were discernible peaks at 2θ values of 15.16\u0026deg; and 21.96\u0026deg; in the XRD analysis of SCB, indicating the existence of the cellulose I. SCBC3 displays prominent peaks at 2\u0026deg;, measuring 15.84\u0026deg; and 22.68\u0026deg;, significantly stronger than in the bagasse. The existence of these peaks is proof that the treatments affected the extracted cellulose. These peak positions imply that the treated fibres' interlunar distance had grown (Liu et al. \u003cspan citationid=\"CR39\" class=\"CitationRef\"\u003e2019\u003c/span\u003e). The crystallinity index was 29.8% and 53.2% for SCB and SCBC3, respectively. Due to its higher amorphous proportion of lignin and hemicellulose, the SCB has low relative crystallinity (Guilherme et al., 2013). The alkali (NaOH) and bleaching (NaOCl) treatment are the causes of the enhanced crystallinity index seen in extracted cellulose (Melesse et al. \u003cspan citationid=\"CR46\" class=\"CitationRef\"\u003e2022\u003c/span\u003e). Alteration in structure and crystallinity of cellulose may be caused as a result of disruption of intra- and inter-chain H-bonding of cellulosic fibrils (Sharma et al. \u003cspan citationid=\"CR63\" class=\"CitationRef\"\u003e2023\u003c/span\u003e and Kundu et al. \u003cspan citationid=\"CR36\" class=\"CitationRef\"\u003e2023\u003c/span\u003e). However, mild reaction conditions did not change the crystalline structure of the cellulose, and the extracted cellulose constituted mainly cellulose I in the structural form (Saad et al. \u003cspan citationid=\"CR58\" class=\"CitationRef\"\u003e2022\u003c/span\u003e).\u003c/p\u003e\u003cp\u003eThe finding of current study is consistent with earlier studies that found that extracting cellulose from sugarcane bagasse increased the crystallinity index. In a study by Yadav et al. (\u003cspan citationid=\"CR80\" class=\"CitationRef\"\u003e2024\u003c/span\u003e), XRD patterns continuously show peaks at roughly 2θ angles of 16.3 and 22.5\u0026deg;. Compared to the untreated SCB biomass, the SCB that had received sodium hydroxide pretreatment displayed a significantly raised peak. The CrI value of the raw SCB was 38.8%. Extracted celulose produced CrI levels of 53.64%. Saad et al. (\u003cspan citationid=\"CR58\" class=\"CitationRef\"\u003e2022\u003c/span\u003e) discovered that the bagasse fibre exhibits two distinct peaks around the 2θ value of 15.33\u0026ordm; and 22.09\u0026deg; and the extracted cellulose showed prominent peaks at 2θ of 16.35\u0026ordm; and 22.47\u0026ordm; that were noticeably stronger. Also the CrI raised from 31.76\u0026ndash;51.13%. The presence of the cellulose I structure is suggested by the peaks that can be seen in the bagasse fibre XRD analysis at 2θ values of 15.22\u0026deg; and 21.94\u0026deg;. Similarly, the extracted cellulose displays distinct peaks at 2\u0026deg;, measuring 15.02\u0026deg; and 22.19\u0026deg;, which are significantly more potent than those in the bagasse (Mubarak et al. \u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). The results of this study are also in agreement with several previous studies that reported an increase in this index value after biomass pretreatment (Galiwangoa et al. 2019;Kininge and Gogate \u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e2022\u003c/span\u003e; Melesse et al. \u003cspan citationid=\"CR46\" class=\"CitationRef\"\u003e2022\u003c/span\u003e; Bangar et al. \u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e2023\u003c/span\u003e; Sharma et al. \u003cspan citationid=\"CR63\" class=\"CitationRef\"\u003e2023\u003c/span\u003e; Sayed \u0026amp; Khalaf \u003cspan citationid=\"CR61\" class=\"CitationRef\"\u003e2023\u003c/span\u003e; Lamo et al. \u003cspan citationid=\"CR38\" class=\"CitationRef\"\u003e2024\u003c/span\u003e; Freitas et al.2024; Rasheed et al. \u003cspan citationid=\"CR55\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). This discovery highlights how alkali treatment affects the cellulose fibres' structural characteristics, which advances our knowledge of their properties and uses.\u003c/p\u003e\u003cdiv id=\"Sec25\" class=\"Section3\"\u003e\u003ch2\u003eFE-SEM\u003c/h2\u003e\u003cp\u003eThe morphological differences between SCB and the extracted cellulose sample SCBC3 treated were analysed using Field emission scanning electron microscopy (FE-SEM), as presented in Fig.\u0026nbsp;\u003cspan refid=\"Fig9\" class=\"InternalRef\"\u003e9\u003c/span\u003e. The FE-SEM image of raw sugarcane bagasse reveals a compact, smooth, and intact surface with minimal visible porosity. This dense outer layer is characteristic of the naturally occurring lignocellulosic matrix, composed predominantly of cellulose, hemicellulose, lignin, waxes, and other extractives (Kumar et al. \u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e2014\u003c/span\u003e; Khalid et al. \u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e2021\u003c/span\u003e). In contrast, the treated sugarcane bagasse cellulose shows a highly porous, fibrillated structure, indicative of effective removal of non-cellulosic components, primarily lignin and hemicellulose (Feleke et al. \u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e2023\u003c/span\u003e; Ding et al. \u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e2025\u003c/span\u003e). Similar changes were seen in the morphological structure of SCB, followed by alkali pretreatment by Yadav et al. (\u003cspan citationid=\"CR80\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). The untreated sample retains intact plant cell wall architecture, with minimal evidence of fibril exposure, suggesting low surface accessibility and reactivity. Conversely, the treated cellulose sample exhibits disrupted cellular networks and partially exposed cellulose microfibrils, suggesting significant structural breakdown and loosening of the plant matrix due to chemical pretreatment (Cao et al. \u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e2006\u003c/span\u003e). A lack of visible porosity in the raw sample indicates the hydrophobic nature and inaccessibility of cellulose chains in their native state. In contrast, the treated sample presents a network of interconnected pores, cracks, and voids, beneficial for applications requiring water absorption or bonding (Velmurugan et al. \u003cspan citationid=\"CR72\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). The increased porosity directly correlates with improved functional properties, such as water uptake, surface modification, and biopolymer reinforcement (Razali et al. \u003cspan citationid=\"CR56\" class=\"CitationRef\"\u003e2022\u003c/span\u003e).\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eOverall FE-SEM analysis confirms that pretreatment of sugarcane bagasse leads to profound morphological changes, especially in surface porosity (Rasheed et al. \u003cspan citationid=\"CR55\" class=\"CitationRef\"\u003e2024\u003c/span\u003e), fibrillation (Phiri et al. \u003cspan citationid=\"CR52\" class=\"CitationRef\"\u003e2024\u003c/span\u003e) and accessibility(Ding et al. 2024), facilitating enhanced surface area (Verma and Goh \u003cspan citationid=\"CR73\" class=\"CitationRef\"\u003e2021\u003c/span\u003e), which is crucial for downstream applications. The results of the current study are also in harmony with the previous studies for SCB treated with acidified NaOCl and alkali treatment by Kumar et al. (\u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e2014\u003c/span\u003e), with acid and alkaline pretreatment and combined hydrothermal and alkaline pretreatment by Guilherme et al. (\u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e2015\u003c/span\u003e), with bleaching agent by Mubarak et al. (\u003cspan citationid=\"CR49\" class=\"CitationRef\"\u003e2024\u003c/span\u003e), with alkaline treatment and hydrogen peroxide bleaching by Rasheed et al. (\u003cspan citationid=\"CR55\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). Additionally, these findings also show consistency with the results of FTIR and XRD, and validate the efficiency of the cellulose extraction process.\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e\u003cdiv id=\"Sec26\" class=\"Section2\"\u003e\u003ch2\u003eStatistical analysis\u003c/h2\u003e\u003cp\u003eResults of statistical analysis revealed that the yield of cellulose was significantly enhanced (p\u0026thinsp;\u0026le;\u0026thinsp;0.5) in SCBC1, SCBC2, SCBC3, SCBC4, and SCBC5 after the amendment with NaOH and NaOCl compared to raw sugarcane bagasse. Meanwhile, SCBC6 did not exhibit a significant change in yield. It was also observed that no significant (p\u0026thinsp;\u0026le;\u0026thinsp;0.5) difference were observed between SCBC2 and SCBC5.\u003c/p\u003e\u003cdiv id=\"Sec27\" class=\"Section3\"\u003e\u003ch2\u003eApplication\u003c/h2\u003e\u003cp\u003eAn intriguing polymeric substance that is widely available on Earth is cellulose and agricultural leftovers are good source of cellulose. It is an auspicious raw material for replacing non-renewable feedstocks because of its widespread availability. Researchers have focused on natural cellulose because of its advantages over synthetic cellulose, which include its availability, affordability, perusability, biocompatibility, and minimal toxicity. It can also undergo chemical modification to enhance its chemical and/or physical characteristics. Commonly appealing uses of cellulose include printing, electronics, packaging, and healthcare materials (Aziz et al. \u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e2022\u003c/span\u003e; Magalhaes et al. 2023). Various applications of cellulose are presented in Fig.\u0026nbsp;\u003cspan refid=\"Fig10\" class=\"InternalRef\"\u003e10\u003c/span\u003e.\u003c/p\u003e\u003cp\u003e\u003c/p\u003e\u003cp\u003eThe literature contains a wealth of research on modifying and using cellulosic materials. The efficient incorporation of SCB cellulose into a hydrogel composite using the gamma irradiation approach was noteworthy. This composite can be utilized as a platform for the adsorption of mercury ions (Khiewsawai et al. \u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). Cellulose was converted to carboxymethylcellulose (CMC) in a different study (Ungprasoot et al. \u003cspan citationid=\"CR70\" class=\"CitationRef\"\u003e2021\u003c/span\u003e). Cellulose extracted from SCB was used to prepare microfibrillated cellulose (MFC). This improved the properties of microfibrillated cellulose, allowing it to be easily shaped into pliable sheets. The increased flexibility provides a straightforward method for creating eco-friendly, sustainable and biodegradable products (Wibowo et al. \u003cspan citationid=\"CR78\" class=\"CitationRef\"\u003e2024\u003c/span\u003e). The conversion of cellulose into hydroxyethyl cellulose has numerous critical industrial applications (Halim, 2014).\u003c/p\u003e\u003cp\u003eUsing sugarcane bagasse, Alirach et al. (\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e2023\u003c/span\u003e) created and characterised bio-sheets for possible application as bio-sheet packaging material. The biosheets had 2.39 kg cm\u003csup\u003e\u0026minus;\u0026thinsp;2\u003c/sup\u003e, 25.3 kgf and 11 kgf bursting strength, strip strength and stiffness, respectively. The results demonstrated that the bio-sheets under optimal conditions possessed sufficient physical qualities. These characteristics imply that the bio-sheets can also be improved to create bio-bags. Using ecologically friendly materials, specifically tapioca starch and sugarcane bagasse fibre (SBF), Asrofi et al. (\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e2020\u003c/span\u003e) attempted to develop composite bioplastic. SBF is added to the tapioca matrix to reinforce the structural integrity of composite bioplastics. The findings demonstrate that adding ultrasonication improves the tensile strength of the composite bioplastic samples. The sample that underwent 15 minutes of ultrasonication at 2.5 MPa had the highest tensile strength. Ali et al. (\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2022\u003c/span\u003e) created a PVA/starch nanocomposite film reinforced with sugarcane bagasse cellulose nanofiber (CNF) as an alternative to the current biodegradable plastic packaging options.\u003c/p\u003e\u003cp\u003eUsing ultrasonication and alkaline and mild acid treatment, cellulose nanofibers (CNF) were separated from sugarcane bagasse (SCB). It enhanced antimicrobial, thermal, and mechanical qualities. Using an N-dimethylacetamide/lithium chloride solvent, sugarcane bagasse nanofibers were converted into an all-cellulose nanocomposite (ACNC) film. The nanofiber sheet, ACNC and fibre sheet produced with a 10-minute dissolution time had respective tensile strengths of 8 MPa, 101 MPa, and 140 MPa. As the dissolution period increased, the ACNC film's water vapour permeability (WVP) rose proportionately. Because of its promising qualities, ACNC may be used in cellulose-based food packaging (Ghadheri et al. 2014). In 2020, Azmin et al. used sugarcane bagasse and cocoa pod husk to create biodegradable plastic films. Future research should concentrate on the environmentally friendly conversion of cellulose and its functional derivatives with increased efficiency and high selectivity.\u003c/p\u003e\u003c/div\u003e\u003c/div\u003e"},{"header":"Conclusion","content":"\u003cp\u003eSugarcane bagasse exhibited great potential for cellulose extraction. The process conditions were optimised to maximise the cellulose yield. The process conditions with 5% NaOH and 1.5% NaOCl were the optimum, with the highest cellulose yield of 56.5\u0026thinsp;\u0026plusmn;\u0026thinsp;0.2%. FTIR analysis revealed the removal of lignin and hemicellulose from sugarcane bagasse after treatment, as the peaks at 1723 cm⁻\u0026sup1;, 1511 cm⁻\u0026sup1; and 1292 cm⁻\u0026sup1; responsible for these components were missing in the extracted cellulose samples. However, the peaks relating to C\u0026ndash;O\u0026ndash;C and O\u0026ndash;H confirmed the presence of cellulose. The XRD results showed an enhancement in cellulose crystallinity following alkali pretreatment. FE-SEM imaging effectively highlighted the structural transformations caused by the treatment, and an increase in surface porosity and accessibility was observed. Thus, it can be concluded that cellulose was successfully isolated from SCB. Statistical analysis also revealed a significant difference (p\u0026thinsp;\u0026le;\u0026thinsp;0.05) in the yield of cellulose after applying the varying concentrations of NaOH and NaOCl as compared to raw SCB. The cellulose generated can find application in biofilm preparation after adding suitable binders. While the current study did not examine the effects of time and temperature, future investigations should explore how changes in these pre-treatment conditions influence the cellulose extraction to achieve a deeper understanding. Further evaluation of the cost-efficiency of these processes will provide critical insights for their adoption in sustainable industrial practices.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003ch2\u003eConflict of Interest\u003c/h2\u003e\u003cp\u003eThe authors declare that they have no competing interests.\u003c/p\u003e\u003c/p\u003e\u003ch2\u003eFunding\u003c/h2\u003e\u003cp\u003eNo funding was received for the preparation of this manuscript.\u003c/p\u003e\u003ch2\u003eAuthor Contribution\u003c/h2\u003e\u003cp\u003eThis study was a collaborative effort, with contributions from all authors. \u003cb\u003eShikha Kumari\u003c/b\u003e conceptualized and designed the study, performed the experiments, and prepared the initial draft of the manuscript. \u003cb\u003eDinesh Arora\u003c/b\u003e conducted the statistical analyses and gave valuable sugestions for the improvement of manuscript. \u003cb\u003eDr. Manjeet Kaur\u003c/b\u003e and \u003cb\u003eDr. Geeta Dhania\u003c/b\u003e thoroughly reviewed the manuscript, offered valuable insights, and finalized the paper for submission. All authors read and approved the final version of the manuscript.\u003c/p\u003e\u003ch2\u003eAcknowledgement\u003c/h2\u003e\u003cp\u003eThe author gratefully acknowledges the support and encouragement provided by the \u003cb\u003eDepartment of Environmental Science, Maharshi Dayanand University, Rohtak, Haryana, India\u003c/b\u003e throughout the course of this research. The facilities, academic environment, and valuable guidance offered by the department played a significant role in the successful completion of this study.\u003c/p\u003e\u003ch2\u003eData Availability Statement\u003c/h2\u003e\u003cp\u003eThis study did not generate or analyze any datasets.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eAbdel-Halim ES (2014) Chemical modification of cellulose extracted from sugarcane bagasse: Preparation of hydroxyethyl cellulose. Arabian J Chem 7(3). https://doi.org/10.1016/j.arabjc.2013.05.006\u003c/li\u003e\n\u003cli\u003eAli MASS, Jimat DN, Nawawi WMFW, Sulaiman S (2022) Antibacterial, mechanical and thermal properties of PVA/starch composite film reinforced with cellulose nanofiber of sugarcane bagasse. Arabian J Sci Eng 47(5). https://doi.org/10.1007/s13369-021-05336-w\u003c/li\u003e\n\u003cli\u003eAlirach V, Lubwama M, Olupot PW, Kukunda L (2023) Development and characterisation of bio-sheets from sugarcane bagasse as a potential packaging material. Biomass Convers Biorefinery. https://doi.org/10.1007/s13399-023-04689-6\u003c/li\u003e\n\u003cli\u003eAlokika A, Anu Kumar A, Kumar V, Singh B (2021) Cellulosic and hemicellulosic fractions of sugarcane bagasse: Potential, challenges and future perspective. Int J Biol Macromol 169. https://doi.org/10.1016/j.ijbiomac.2020.12.175\u003c/li\u003e\n\u003cli\u003eAshokkumar V, Rajendran S, Balakrishnan M, Singh S, Pradeep Kumar R, Jayaraman R (2022) Recent advances in lignocellulosic biomass for biofuels and value-added bioproducts \u0026ndash; a critical review. Bioresour Technol 344:126195. https://doi.org/10.1016/j.biortech.2021.126195\u003c/li\u003e\n\u003cli\u003eAsrofi M, Sapuan SM, Ilyas RA, Ramesh M (2020) Characteristic of composite bioplastics from tapioca starch and sugarcane bagasse fiber: Effect of time duration of ultrasonication (Bath-Type). Mater Today: Proc 46. https://doi.org/10.1016/j.matpr.2020.07.254\u003c/li\u003e\n\u003cli\u003eAziz T, Farid A, Haq F, Kiran M, Ullah A, Zhang K, Li C, Ghazanfar S, Sun H, Ullah R, Ali A, Muzammal M, Shah M, Akhtar N, Selim S, Hagagy N, Samy M, al Jaouni SK (2022) A Review on the Modification of Cellulose and Its Applications. Polymers 14(15). https://doi.org/10.3390/polym14153206\u003c/li\u003e\n\u003cli\u003eAzmin SNHM, Hayat NABM, Nor MSM (2020) Development and characterization of food packaging bioplastic film from cocoa pod husk cellulose incorporated with sugarcane bagasse fibre. J Bioresour Bioproducts 5(4). https://doi.org/10.1016/j.jobab.2020.10.003\u003c/li\u003e\n\u003cli\u003eBalasubramani V, Nagarajan KJ, Karthic M, Pandiyarajan R (2024) Extraction of lignocellulosic fiber and cellulose microfibrils from agro waste-palmyra fruit peduncle: Water retting, chlorine-free chemical treatments, physio-chemical, morphological, and thermal characterization. Int J Biol Macromol 259. https://doi.org/10.1016/j.ijbiomac.2024.129273\u003c/li\u003e\n\u003cli\u003eBangar SP, Whiteside WS, Kajla P, Tavassoli M (2023) Value addition of rice straw cellulose fibers as a reinforcer in packaging applications. Int J Biol Macromol 243. https://doi.org/10.1016/j.ijbiomac.2023.125320\u003c/li\u003e\n\u003cli\u003eBen F, Olubambi PA (2024) Investigating the tribological behavior of bioinspired surfaces in agro-waste and alumina reinforced AA6063 matrix composites. Vacuum 230:113687. https://doi.org/10.1016/j.vacuum.2024.113687\u003c/li\u003e\n\u003cli\u003eBledzki AK, Mamun AA, Volk J (2010) Physical, chemical and surface properties of wheat husk, rye husk and soft wood and their polypropylene composites. Compos Part A Appl Sci Manuf 41(4):480\u0026ndash;488\u003c/li\u003e\n\u003cli\u003eCao Y, Shibata S, Fukumoto I (2006) Mechanical properties of biodegradable composites reinforced with bagasse fibre before and after alkali treatments. Composites Part A: Appl Sci Manuf 37(3). https://doi.org/10.1016/j.compositesa.2005.05.045\u003c/li\u003e\n\u003cli\u003eDebnath B, Haldar D, Purkait MK (2021) A critical review on the techniques used for the synthesis and applications of crystalline cellulose derived from agricultural wastes and forest residues. Carbohydr Polym 273. https://doi.org/10.1016/j.carbpol.2021.118537\u003c/li\u003e\n\u003cli\u003edel Sole R, Mele G, Bloise E, Mergola L (2021) Green aspects in molecularly imprinted polymers by biomass waste utilization. Polymers 13(15). https://doi.org/10.3390/polym13152430\u003c/li\u003e\n\u003cli\u003eDing K, Yao Z, Yimamu N, Aydan T, Guan Q (2025) Optimization and characterization of cellulose extraction from cotton straw using alkali pretreatment. Biomass Convers Biorefinery 1\u0026ndash;14. https://doi.org/10.1007/s13399-025-05036-6\u003c/li\u003e\n\u003cli\u003eFeleke K, Thothadri G, Beri Tufa H, Rajhi AA, Ahmed GMS (2023) Extraction and Characterization of Fiber and Cellulose from Ethiopian Linseed Straw: Determination of Retting Period and Optimization of Multi-Step Alkaline Peroxide Process. Polymers 15(2). https://doi.org/10.3390/polym15020469\u003c/li\u003e\n\u003cli\u003eFreitas PAV, Gonz\u0026aacute;lez-Mart\u0026iacute;nez C, Chiralt A (2022) Applying ultrasound-assisted processing to obtain cellulose fibres from rice straw to be used as reinforcing agents. Innov Food Sci Emerg Technol 76. https://doi.org/10.1016/j.ifset.2022.102932\u003c/li\u003e\n\u003cli\u003eFreitas PAV, Santana LG, Gonz\u0026aacute;lez-Mart\u0026iacute;nez C, Chiralt A (2024) Combining subcritical water extraction and bleaching with hydrogen peroxide to obtain cellulose fibres from rice straw. Carbohydr Polym Technol Appl 7. https://doi.org/10.1016/j.carpta.2024.100491\u003c/li\u003e\n\u003cli\u003eGaliwango E, Abdel Rahman NS, Al-Marzouqi AH, Abu-Omar MM, Khaleel AA (2019) Isolation and characterization of cellulose and \u0026alpha;-cellulose from date palm biomass waste. Heliyon 5(12). https://doi.org/10.1016/j.heliyon.2019.e02937\u003c/li\u003e\n\u003cli\u003eGangil S, Wakudkar HM (2013) Generation of bio-char from crop residues. Int J Emerg Technol Adv Eng 3:566\u0026ndash;570\u003c/li\u003e\n\u003cli\u003eGhaderi M, Mousavi M, Yousefi H, Labbafi M (2014) All-cellulose nanocomposite film made from bagasse cellulose nanofibers for food packaging application. Carbohydr Polym 104(1). https://doi.org/10.1016/j.carbpol.2014.01.013\u003c/li\u003e\n\u003cli\u003eGuilherme AA, Dantas PVF, Santos ES, Fernandes FAN, Macedo GR (2015) Evaluation of composition, characterization and enzymatic hydrolysis of pretreated sugar cane bagasse. Braz J Chem Eng 32(1). https://doi.org/10.1590/0104-6632.20150321s00003146\u003c/li\u003e\n\u003cli\u003eHan W, Geng Y (2023) Optimization and characterization of cellulose extraction from olive pomace. Cellulose 30(8). https://doi.org/10.1007/s10570-023-05195-8\u003c/li\u003e\n\u003cli\u003eHashim MY, Amin AM, Marwah OMF, Othman MH, Yunus MR, Chuan Huat N (2017) The effect of alkali treatment under various conditions on physical properties of kenaf fiber. J Phys Conf Ser 914(1). https://doi.org/10.1088/1742-6596/914/1/012030\u003c/li\u003e\n\u003cli\u003eJung W, Savithri D, Sharma-Shivappa R, Kolar P (2020) Effect of Sodium Hydroxide Pretreatment on Lignin Monomeric Components of Miscanthus \u0026times; giganteus and Enzymatic Hydrolysis. Waste Biomass Valorization 11(11). https://doi.org/10.1007/s12649-019-00859-8\u003c/li\u003e\n\u003cli\u003eKadla JF, Gilbert RD (2000) Cellulose structure: A review. Cellul Chem Technol 34(3\u0026ndash;4):197\u0026ndash;216\u003c/li\u003e\n\u003cli\u003eKapdi D, Bhavsar N, Rudakiya D (2024) Developing sustainable and energy efficient process to obtain high purity cellulose and its value-added chemicals from rice straw. Bioresour Technol Rep 25. https://doi.org/10.1016/j.biteb.2023.101732\u003c/li\u003e\n\u003cli\u003eKatakojwala R, Mohan SV (2020) Microcrystalline cellulose production from sugarcane bagasse: Sustainable process development and life cycle assessment. J Clean Prod 249. https://doi.org/10.1016/j.jclepro.2019.119342\u003c/li\u003e\n\u003cli\u003eKathirselvam M, Kumaravel A, Arthanarieswaran VP, Saravanakumar SS (2019) Characterization of cellulose fibers in \u003cem\u003eThespesia populnea\u003c/em\u003e barks: Influence of alkali treatment. Carbohydr Polym 217:178\u0026ndash;189. https://doi.org/10.1016/j.carbpol.2019.04.038\u003c/li\u003e\n\u003cli\u003eKhalid AM, Hossain MS, Ismail N et al (2021) Isolation and characterization of magnetic oil palm empty fruits bunch cellulose nanofiber composite as a bio-sorbent for Cu(II) and Cr(VI) removal. Polymers (Basel) 13:1\u0026ndash;22. https://doi.org/10.3390/polym13010112\u003c/li\u003e\n\u003cli\u003eKhiewsawai N, Rattanawongwiboon T, Ummartyotin S (2024) Cellulose fiber derived from sugarcane bagasse and polyethylene glycol/acrylic acid/branched polyethylenimine-based hydrogel composite prepared by gamma irradiation: A platform for mercury (II) ions adsorption. Environ Adv 17:100561. https://doi.org/10.1016/j.envadv.2024.100561\u003c/li\u003e\n\u003cli\u003eKhui PLN, Rahman MR, Bakri MKB (2021) A review on the extraction of cellulose and nanocellulose as a filler through solid waste management. J Thermoplast Compos Mater. https://doi.org/10.1177/08927057211020800\u003c/li\u003e\n\u003cli\u003eKininge MM, Gogate PR (2022) Intensification of alkaline delignification of sugarcane bagasse using ultrasound assisted approach. Ultrason Sonochem 82:105870. https://doi.org/10.1016/j.ultsonch.2021.105870\u003c/li\u003e\n\u003cli\u003eKumar A, Ewing AH, Wereley ST (2014) Optical tweezers for manipulating cells and particles. In: Encyclopedia of Microfluidics and Nanofluidics (pp 1\u0026ndash;8). Springer US. https://doi.org/10.1007/978-3-642-27758-0_1162-2\u003c/li\u003e\n\u003cli\u003eKundu S, Mitra D, Das M (2023) Influence of chlorite treatment on the fine structure of alkali pretreated sugarcane bagasse. Biomass Convers Biorefinery 13(2). https://doi.org/10.1007/s13399-020-01120-2\u003c/li\u003e\n\u003cli\u003eLaluce C, Roldan IU, Pecoraro E, Igbojionu LI, Ribeiro CA (2019) Effects of pretreatment applied to sugarcane bagasse on composition and morphology of cellulosic fractions. Biomass Bioenergy 126:231\u0026ndash;238. https://doi.org/10.1016/j.biombioe.2019.05.015\u003c/li\u003e\n\u003cli\u003eLamo C, Bargale PC, Gangil S, Chakraborty S, Tripathi MK, Kotwaliwale N, Modhera B (2024) High crystalline cellulose extracted from chickpea husk using alkali treatment. Biomass Convers Biorefinery 14(1). https://doi.org/10.1007/s13399-022-02331-5\u003c/li\u003e\n\u003cli\u003eLiu X, Jiang Y, Song X, Qin C, Wang S, Li K (2019) A bio-mechanical process for cellulose nanofiber production \u0026ndash; Towards a greener and energy conservation solution. Carbohydr Polym 208. https://doi.org/10.1016/j.carbpol.2018.12.071\u003c/li\u003e\n\u003cli\u003eLoh YR, Sujan D, Rahman ME, Das CA (2013) Review Sugarcane bagasse - The future composite material: A literature review. Resour Conserv Recycl 75. https://doi.org/10.1016/j.resconrec.2013.03.002\u003c/li\u003e\n\u003cli\u003eLou C, Zhou Y, Yan A, Liu Y (2022) Extraction cellulose from corn-stalk taking advantage of pretreatment technology with immobilized enzyme. RSC Adv 12(2). https://doi.org/10.1039/d1ra07513f\u003c/li\u003e\n\u003cli\u003eLu P, Hsieh Ylo (2012) Cellulose isolation and core-shell nanostructures of cellulose nanocrystals from chardonnay grape skins. Carbohydr Polym 87(4). https://doi.org/10.1016/j.carbpol.2011.11.023\u003c/li\u003e\n\u003cli\u003eMagalh\u0026atilde;es S, Fernandes C, Pedrosa JFS, Alves L, Medronho B, Ferreira PJT, Rasteiro MdG (2023) Eco-friendly methods for extraction and modification of cellulose: An overview. Polymers 15(14). https://doi.org/10.3390/polym15143138\u003c/li\u003e\n\u003cli\u003eMahmud MA, Anannya FR (2021) Sugarcane bagasse - A source of cellulosic fiber for diverse applications. Heliyon 7(8). https://doi.org/10.1016/j.heliyon.2021.e07771\u003c/li\u003e\n\u003cli\u003eMartin-Bertelsen B, Andersson E, K\u0026ouml;hnke T, Hedlund A, Stigsson L, Olsson U (2020) Revisiting the dissolution of cellulose in NaOH as \u0026ldquo;Seen\u0026rdquo; by X-rays. Polymers 12(2). https://doi.org/10.3390/polym12020342\u003c/li\u003e\n\u003cli\u003eMelesse GT, Hone FG, Mekonnen MA (2022) Extraction of cellulose from sugarcane bagasse optimization and characterization. Adv Mater Sci Eng 2022. https://doi.org/10.1155/2022/1712207\u003c/li\u003e\n\u003cli\u003eMohammed M, Oleiwi JK, Mohammed AM, Jawad AJafar M, Osman AF, Adam T, Betar BO, Gopinath SCB, Dahham OS, Jaafar M (2023) Comprehensive insights on mechanical attributes of natural-synthetic fibres in polymer composites. J Mater Res Technol 25. https://doi.org/10.1016/j.jmrt.2023.06.148\u003c/li\u003e\n\u003cli\u003eMoubarik A, Grimi N, Boussetta N (2013) Structural and thermal characterization of Moroccan sugar cane bagasse cellulose fibers and their applications as a reinforcing agent in low density polyethylene. Compos Part B Eng 52:233\u0026ndash;238. https://doi.org/10.1016/j.compositesb.2013.04.012\u003c/li\u003e\n\u003cli\u003eMubarak AA, Ilyas RA, Ngadi N, Nordin AH, Alkbir FM (2024) Isolation and characterization of cellulose from sugarcane bagasse fiber (\u003cem\u003eSaccharum officinarum\u003c/em\u003e) via delignification and mercerization treatment using response surface modeling (RSM). Biomass Convers Biorefinery. https://doi.org/10.1007/s13399-024-05692-1\u003c/li\u003e\n\u003cli\u003eNandiyanto ABD, Oktiani R, Ragadhita R (2019) How to read and interpret FTIR spectroscope of organic material. Indones J Sci Technol 4(1). https://doi.org/10.17509/ijost.v4i1.15806\u003c/li\u003e\n\u003cli\u003ePerumal AB, Sellamuthu PS, Nambiar RB, Sadiku ER (2018) Development of polyvinyl alcohol/chitosan bio-nanocomposite films reinforced with cellulose nanocrystals isolated from rice straw. Appl Surf Sci 449. https://doi.org/10.1016/j.apsusc.2018.01.022\u003c/li\u003e\n\u003cli\u003ePhiri R, Rangappa SM, Andoko A, Gapsari F, Siengchin S (2024) Modification of cellulose in sugarcane bagasse fibers towards development of biocomposite. Biomass Convers Biorefinery 1\u0026ndash;17. https://doi.org/10.1007/s13399-024-05702-9\u003c/li\u003e\n\u003cli\u003eRahayu A, Hanum FF, Amrillah NAZ, Lim LW, Salamah S (2022) Cellulose extraction from coconut coir with alkaline delignification process. J Fibers Polym Compos 1(2). https://doi.org/10.55043/jfpc.v1i2.51\u003c/li\u003e\n\u003cli\u003eRana V, Malik S, Joshi G, Rajput NK, Gupta PK (2021) Preparation of alpha cellulose from sugarcane bagasse and its cationization: Synthesis, characterization, validation and application as wet-end additive. Int J Biol Macromol 170. https://doi.org/10.1016/j.ijbiomac.2020.12.165\u003c/li\u003e\n\u003cli\u003eRasheed HA, Adeleke AA, Nzerem P, Olosho AI, Ogedengbe TS, Jesuloluwa S (2024) Isolation, characterization and response surface method optimization of cellulose from hybridized agricultural wastes. Sci Rep 14(1). https://doi.org/10.1038/s41598-024-65229-4\u003c/li\u003e\n\u003cli\u003eRazali NAM, Sohaimi RM, Othman RNIR, Abdullah N, Demon SZN, Jasmani L, Yunus WMZW, Ya\u0026rsquo;acob WMHW, Salleh EM, Norizan MN, Halim NA (2022) Comparative study on extraction of cellulose fiber from rice straw waste from chemo-mechanical and pulping method. Polymers 14(3). https://doi.org/10.3390/polym14030387\u003c/li\u003e\n\u003cli\u003eRizwan M, Gilani SR, Durrani AI, Naseem S (2021) Cellulose extraction of \u003cem\u003eAlstonia scholaris\u003c/em\u003e: A comparative study on efficiency of different bleaching reagents for its isolation and characterization. Int J Biol Macromol 191. https://doi.org/10.1016/j.ijbiomac.2021.09.155\u003c/li\u003e\n\u003cli\u003eSaad AA, Ahmed HS, Megally IAN, Ahmed M, Ibraheem MT, Farouk SM, Khalaf ESA (2022) Optimization and characterization of cellulose extracted from sugarcane bagasse. In: The International Undergraduate Research Conference, Vol. 6(6), pp 1\u0026ndash;9. The Military Technical College\u003c/li\u003e\n\u003cli\u003eSant\u0026rsquo;Ana da Silva A, Lee SH, Endo T, Bon EPS (2011) Major improvement in the rate and yield of enzymatic saccharification of sugarcane bagasse via pretreatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate ([Emim][Ac]). Bioresour Technol 102(22). https://doi.org/10.1016/j.biortech.2011.08.085\u003c/li\u003e\n\u003cli\u003eSayakulu NF, Soloi S (2022) The effect of sodium hydroxide (NaOH) concentration on oil palm empty fruit bunch (OPEFB) cellulose yield. J Phys Conf Ser 2314(1). https://doi.org/10.1088/1742-6596/2314/1/012017\u003c/li\u003e\n\u003cli\u003eSayed E, Khalaf A (2023) Optimization and characterization of cellulose extracted from sugarcane bagasse. Res Sq 1\u0026ndash;15. https://doi.org/10.21203/rs.3.rs-2618002/v1\u003c/li\u003e\n\u003cli\u003eShaikh HM, Pandare KV, Nair G, Varma AJ (2009) Utilization of sugarcane bagasse cellulose for producing cellulose acetates: Novel use of residual hemicellulose as plasticizer. Carb Polym. 2;76(1):23-9.\u003c/li\u003e\n\u003cli\u003eSharma V, Nargotra P, Sharma S, Sawhney D, Vaid S, Bangotra R, Dutt HC, Bajaj BK (2023) Microwave irradiation-assisted ionic liquid or deep eutectic solvent pretreatment for effective bioconversion of sugarcane bagasse to bioethanol. Energy Ecol Environ 8(2). https://doi.org/10.1007/s40974-022-00267-0\u003c/li\u003e\n\u003cli\u003eSong K, Zhu X, Zhu W, Li X (2019) Preparation and characterization of cellulose nanocrystal extracted from \u003cem\u003eCalotropis procera\u003c/em\u003e biomass. Bioresour Bioprocess 6(1). https://doi.org/10.1186/s40643-019-0279-z\u003c/li\u003e\n\u003cli\u003eStevenson L, Phillips F, O\u0026rsquo;Sullivan K, Walton J (2012) Wheat bran: Its composition and benefits to health, a European perspective. Int J Food Sci Nutr 63(8). https://doi.org/10.3109/09637486.2012.687366\u003c/li\u003e\n\u003cli\u003eSun JX, Sun XF, Zhao H, Sun RC (2004) Isolation and characterization of cellulose from sugarcane bagasse. Polym Degrad Stab 84(2):331\u0026ndash;339. https://doi.org/10.1016/j.polymdegradstab.2004.02.008\u003c/li\u003e\n\u003cli\u003eSun S, Sun S, Cao X, Sun R (2016) The role of pretreatment in improving the enzymatic hydrolysis of lignocellulosic materials. Bioresour Technol 199. https://doi.org/10.1016/j.biortech.2015.08.061\u003c/li\u003e\n\u003cli\u003eTAPPI T 222 om-02 (2002) Acid-insoluble lignin in wood and pulp. TAPPI Test Methods.\u003c/li\u003e\n\u003cli\u003eTAPPI T204 cm-17 (2017) Solvent Extractives of Wood and Pulp. TAPPI Test Methods.\u003c/li\u003e\n\u003cli\u003eUngprasoot P, Muanruksa P, Tanamool V, Winterburn J, Kaewkannetra P (2021) Valorization of aquatic weed and agricultural residues for innovative biopolymer production and their biodegradation. Polymers 13(17):2838. https://doi.org/10.3390/polym13172838\u003c/li\u003e\n\u003cli\u003eUzoh Raymond D, Jildawa D, Sudi P (2024) Extraction and characterization of cellulose from agricultural biomass (sugarcane bagasse). Int J Model Appl Sci Res 3(9). https://cambridgeresearchpub.com/ijmasr/article/view/194\u003c/li\u003e\n\u003cli\u003eVelmurugan T, Suganya Priyadharshini G, Suyambulingam I, Siengchin S (2024) Extraction and characterization of \u003cem\u003eThespesia populnea\u003c/em\u003e leaf cellulose: a biomass to biomaterial conversion. \u003cem\u003eBiomass Conversion and Biorefinery\u003c/em\u003e (published online: 7 August 2024). Springer-Verlag GmbH Germany, part of Springer Nature. https://doi.org/10.1007/s13399-024-05705-6\u003c/li\u003e\n\u003cli\u003eVerma D, Goh KL (2021) Effect of mercerization/alkali surface treatment of natural fibres and their utilization in polymer composites: mechanical and morphological studies. J Compos Sci 5:175. https://doi.org/10.3390/jcs5070175\u003c/li\u003e\n\u003cli\u003eViera RGP, Filho GR, de Assun\u0026ccedil;\u0026atilde;o RMN, Carla Cd, Vieira JG, de Oliveira GS (2007) Synthesis and characterization of methylcellulose from sugar cane bagasse cellulose. Carbohydr Polym 67(2). https://doi.org/10.1016/j.carbpol.2006.05.007\u003c/li\u003e\n\u003cli\u003eWang W, Wang X, Zhang Y, Yu Q, Tan X, Zhuang X, Yuan Z (2020) Effect of sodium hydroxide pretreatment on physicochemical changes and enzymatic hydrolysis of herbaceous and woody lignocelluloses. Ind Crops Prod 145. https://doi.org/10.1016/j.indcrop.2020.112145\u003c/li\u003e\n\u003cli\u003eWang X, Yang G, Feng Y, Ren G, Han X (2012) Optimizing feeding composition and carbon-nitrogen ratios for improved methane yield during anaerobic co-digestion of dairy, chicken manure and wheat straw. Bioresour Technol 120. https://doi.org/10.1016/j.biortech.2012.06.058\u003c/li\u003e\n\u003cli\u003eWang Z, Qiao X, Sun K (2018) Rice straw cellulose nanofibrils reinforced poly(vinyl alcohol) composite films. Carbohydr Polym 197. https://doi.org/10.1016/j.carbpol.2018.06.025\u003c/li\u003e\n\u003cli\u003eWibowo A, Alandro D, Killian MS, Nugroho G, Raghu SNV, Muflikhun AM (2024) Mechanical evaluation and characterization of hybrid sugarcane bagasse microfibrillated cellulose with added filler materials for use as disposable utensils. Adv Compos Mater 33(1). https://doi.org/10.1080/09243046.2023.2180793\u003c/li\u003e\n\u003cli\u003eWise LE, Murphy M, D\u0026rsquo;Addieco AA (1946) Chlorite holocellulose: Its fractionation and bearing on summative wood analysis and on studies on the hemicellulose. Paper Trade J 122:35\u0026ndash;43.\u003c/li\u003e\n\u003cli\u003eYadav A, Rani P, Yadav DK, Bhardwaj N, Gupta A, Bishnoi NR (2024) Enhancing enzymatic hydrolysis and delignification of sugarcane bagasse using different concentrations of sodium alkaline pretreatment. Nat Environ Pollut Technol 23(1):427\u0026ndash;434. https://doi.org/10.46488/NEPT.2024.v23i01.037\u003c/li\u003e\n\u003cli\u003eZhao T, Chen Z, Lin X, Ren Z, Li B, Zhang Y (2018) Preparation and characterization of microcrystalline cellulose (MCC) from tea waste. Carbohydr Polym 184. https://doi.org/10.1016/j.carbpol.2017.12.024\u003c/li\u003e\n\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":true,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"bioenergy-research","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bere","sideBox":"Learn more about [BioEnergy Research](https://www.springer.com/journal/12155)","snPcode":"12155","submissionUrl":"https://submission.nature.com/new-submission/12155/3","title":"BioEnergy Research","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Agricultural residue, Cellulose, Sugarcane Bagasse, Alkaline treatment, Bleaching","lastPublishedDoi":"10.21203/rs.3.rs-7360666/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7360666/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eAs the world seeks sustainable alternatives to fossil-based materials, agricultural residues like sugarcane bagasse are gaining prominence as valuable bioresources. Sugarcane bagasse, the fibrous waste left after juice extraction, is an agricultural residue that is particularly rich in cellulose, a biopolymer with immense potential for green material innovation. The present work utilised sugarcane bagasse, to obtain cellulose by optimising the Sodium hydroxide (NaOH) and Sodium hypochlorite (NaOCl) concentrations. Characterisation techniques such as \u0026nbsp;Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscope (FE-SEM) and X-ray Diffraction (XRD) were employed for detailed analysis. This article also encloses the effect of NaOH and NaOCl concentration on the cellulose yield and the various possible applications of the obtained cellulose. To evaluate statistical significance, one-way ANOVA was performed, complemented by Tukey’s multiple comparison test. Compositional analysis showed that the cellulose content of raw sugarcane bagasse was 42.6±1.5%. However, after alkali and bleaching treatment, the cellulose content was in the range 42.6±0.6% - 56.5±0.5%. FTIR analysis confirmed the successful cellulose extraction from sugarcane bagasse, as evidenced by the disappearance of lignin and hemicellulose associated peaks and characteristic cellulose absorption bands. XRD analysis revealed an increase in the crystallinity index from 29.8% in SCB to 53.7% in extracted cellulose. Morphological analysis employing FE-SEM highlighted significant surface differences in SCB and extracted cellulose. Statistical analysis unfolded that amendment with NaOH and NaOCl enhanced the cellulose yield significantly (p≤0.05) compared to raw sugarcane bagasse. The study highlights the immense potential of agricultural waste as a renewable and cost-effective source of cellulose. By leveraging these residues, industries can reduce dependence on conventional raw materials while promoting sustainable and environmentally responsible practices.\u003c/p\u003e","manuscriptTitle":"Enhanced Cellulose Isolation from Sugarcane Bagasse through Sequential Alkali and Oxidative Treatment","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-10-09 13:08:47","doi":"10.21203/rs.3.rs-7360666/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Reconsider pending major revisions","date":"2026-03-01T13:00:41+00:00","index":"","fulltext":""},{"type":"reviewerAgreed","content":"","date":"2025-10-13T01:58:42+00:00","index":0,"fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-09-28T15:49:16+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"BioEnergy Research","date":"2025-08-30T20:10:24+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-08-27T03:21:37+00:00","index":"","fulltext":""},{"type":"submitted","content":"BioEnergy Research","date":"2025-08-13T00:27:52+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"bioenergy-research","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"bere","sideBox":"Learn more about [BioEnergy Research](https://www.springer.com/journal/12155)","snPcode":"12155","submissionUrl":"https://submission.nature.com/new-submission/12155/3","title":"BioEnergy Research","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"d9680acc-c671-49a7-a026-c8f874c90d30","owner":[],"postedDate":"October 9th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"under-review","subjectAreas":[],"tags":[],"updatedAt":"2026-04-12T22:28:36+00:00","versionOfRecord":[],"versionCreatedAt":"2025-10-09 13:08:47","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7360666","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7360666","identity":"rs-7360666","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}