Effects of Two Isolated Compounds from Red Sorghum Bran on p53 and bcl 2 Gene Expression in Human Breast cancer Cell Line (MCF-7)

Aims: : The aim of our research was to isolate anthocyanin from red sorghum bran and to study its effects on p 53 and bcl 2 gene expressions in Human Breast cancer cell line (MCF-7). Main methods: Two compounds were isolated from red sorghum bran by using Sephadex LH-20 column chromatography. The antiproliferative activity of isolated compounds were analysed by MTT assay and the expression studies were performed by RT-PCR analysis. Key findings: Our research focusing on apigenin-5-β-D-glucopyranoside and luteolin-5-β-D-glucopyranoside another compound as a potention anticancer agent have demonstrated that the compounds isolated from red sorghum bran inhibited breast cancer cell lines by up regulation of P 53 gene expression and down regulation of Bcl 2 gene expression. Significance: In conclusion, the two compounds isolated from red sorghum bran, were effective in up regulation of P 53 and down regulation of Bcl 2 gene expression.