Self-powered hydrogel improves bone regeneration via bone drived biomechanical piezoelectricity

Self-powered hydrogel improves bone regeneration via bone drived biomechanical piezoelectricity | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Self-powered hydrogel improves bone regeneration via bone drived biomechanical piezoelectricity Yulin Li, Shuyan Zhang, Lei Huang, Weisin Chen, Qi Chen, Xin Liu, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4971787/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Large bone defect healing remains a challenge in current clinical treatment, which suggests the need for functional bone repair materials. Piezoelectric materials can generate electrical stimulation under mechanical stress to improve the tissue healing environment, which are emerging candidates for tissue engineering. We created a self-powered piezoelectric hydrogel by simply blending the zinc oxide (ZnO) nanoparticles and regenerating silk fibroin (RSF). Our piezoelectric hydrogel showed controllable and excellent mechanical and piezoelectric properties which could generate electrical stimulation to promote bone tissue healing. Incorporating ZnO into RSF hydrogels not only enhanced their mechanical properties by 1.7 times and increased piezoelectric output by 2.8 times, but also mitigated the degradation rate. In vitro experiments showed that piezoelectric hydrogels significantly promoted osteogenesis differentiation of bone mesenchymal stem cells (BMSCs) and enhanced vascular network reconstitution. In vivo experiments verified the osteogenic and angiogenic potential of ZnO/RSF piezoelectric hydrogels. ZnO/RSF piezoelectric hydrogel, a simple but universal strategy of RSF-based material to generate electric currents by body movement, provides new insight into piezoelectric hydrogel application and bone tissue engineering. Physical sciences/Materials science/Biomaterials/Biomaterials – proteins Physical sciences/Materials science/Biomaterials/Biomedical materials regenerated silk fibroin stable piezoelectricity mechanical properties osseointegration angiogenesis Full Text Additional Declarations There is NO Competing Interest. Supplementary Files SIVideo.mp4 Piezoelectric device for in vitro cellular experiments supportinginformation.pdf Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4971787","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":347033749,"identity":"84934ae9-fa4e-4250-ae0c-efd7a5d1ae3f","order_by":0,"name":"Yulin 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