Quantitative Determination of Nifurtimox in Pharmaceutical Preparations by High-Performance Liquid Chromatography

Quantitative Determination of Nifurtimox in Pharmaceutical Preparations by High-Performance Liquid Chromatography | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Quantitative Determination of Nifurtimox in Pharmaceutical Preparations by High-Performance Liquid Chromatography Toshiyuki Chikuma, Tomoaki Sakamoto This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4645096/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract A quantification method for the determination of nifurtimox, which is a chemotherapeutic agent for Chagas disease, in tablet formulations is described. Nifurtimox is dissolved or extracted using 1:1 water/acetonitrile (v/v). Separation was carried out by high-performance liquid chromatography with ultraviolet detection (HPLC–UV) using a C-18 reversed-phase column with isocratic elution. The method was validated according to the ICH guidelines for parameters such as linearity and precision. The detection limit of this method is as low as 25 ng mL−1, and this method yields highly reproducible results. No interference was detected in the chromatogram, and the method was validated by analyzing the tablets. The recoveries were close to 100%. Health sciences/Health care Physical sciences/Chemistry Nifurtimox High-performance liquid chromatography Pharmaceutical formulations Chagas disease Full Text Additional Declarations Table 1 and 2 are available in the Supplementary Files section. Supplementary Files NifurtimoxTable1.xlsx NifurtimoxTable2.xlsx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4645096","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":330136074,"identity":"83a01496-3dbd-428d-8942-81c634ebd3d5","order_by":0,"name":"Toshiyuki Chikuma","email":"","orcid":"","institution":"National Institute of Health Sciences","correspondingAuthor":false,"prefix":"","firstName":"Toshiyuki","middleName":"","lastName":"Chikuma","suffix":""},{"id":330136075,"identity":"2491bbff-1dea-401b-b497-9a77ba7bd430","order_by":1,"name":"Tomoaki Sakamoto","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA2klEQVRIiWNgGAWjYHACZjDJz8DYBhU4gF89D1SLhGQDyVoMDjCwEecqe/7Djw1+5tjUGd9ubnv4g6FWjoHxLH5reCTSjBN7t6VJmN052G7Mw3DcmIHhXAIBLQzGB3i3HZYwu5HYJs3AcCyxgeGMAX4t/Mc/H/wL1GI8I7FN8gdRWhhyjJNBthhIJLZJ8DDUEKHlRk6xsey2NMkZIIfxGBwwZiPkF/b+45sl326z4eefkf5M8kdFnRy/BIEQQwMGhxnYJM6QooOBoQ6YdHpI0zIKRsEoGAXDHgAAMYVDuxj6ksUAAAAASUVORK5CYII=","orcid":"","institution":"National Institute of Health Sciences","correspondingAuthor":true,"prefix":"","firstName":"Tomoaki","middleName":"","lastName":"Sakamoto","suffix":""}],"badges":[],"createdAt":"2024-06-26 23:23:39","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4645096/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4645096/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":61832108,"identity":"cd66b554-c444-46a3-a131-a2018ef907de","added_by":"auto","created_at":"2024-08-06 04:46:58","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":269303,"visible":true,"origin":"","legend":"","description":"","filename":"QuantitativeDeterminationofNifurtimoxinPharmaceuticalPreperationforsubmissionRev.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4645096/v1_covered_6cdd6aa6-b557-4028-80b6-0a8dac89f391.pdf"},{"id":60874293,"identity":"9b32ee4c-ce10-4287-adfe-33387aa61568","added_by":"auto","created_at":"2024-07-23 05:30:30","extension":"xlsx","order_by":0,"title":"","display":"","copyAsset":false,"role":"supplement","size":17956,"visible":true,"origin":"","legend":"","description":"","filename":"NifurtimoxTable1.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-4645096/v1/5e9055df17da99331a8ece7a.xlsx"},{"id":60874292,"identity":"e8061b8d-6181-407d-8986-971ac76eeaf2","added_by":"auto","created_at":"2024-07-23 05:30:29","extension":"xlsx","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":11090,"visible":true,"origin":"","legend":"","description":"","filename":"NifurtimoxTable2.xlsx","url":"https://assets-eu.researchsquare.com/files/rs-4645096/v1/366d868c32e0ec51d9da336e.xlsx"}],"financialInterests":"\u003cp\u003eTable 1 and 2 are available in the Supplementary Files section.\u003c/p\u003e","formattedTitle":"Quantitative Determination of Nifurtimox in Pharmaceutical Preparations by High-Performance Liquid Chromatography","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Nifurtimox, High-performance liquid chromatography, Pharmaceutical formulations, Chagas disease","lastPublishedDoi":"10.21203/rs.3.rs-4645096/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4645096/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"A quantification method for the determination of nifurtimox, which is a chemotherapeutic agent for Chagas disease, in tablet formulations is described. 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