Removal of evolutionarily conserved functional MYC domains in a tilapia cell line using a vector-based CRISPR/Cas9 system

MYC transcription factors have critical roles in facilitating a variety of cellular functions that have been highly conserved among species during evolution. However, despite circumstantial evidence for an involvement of MYC in animal osmoregulation, mechanistic links between MYC function and osmoregulation are missing. Mozambique tilapia ( Oreochromis mossambicus ) represents an excellent model system to study these links because it is highly euryhaline and highly tolerant to osmotic (salinity) stress at both the whole organism and cellular levels of biological organization. Here, we utilize an O. mossambicus brain cell line (OmB cells) and an optimized vector-based CRISPR/Cas9 system to functionally knockout MYC in the tilapia genome and to establish causal links between MYC and cell function, including cellular osmoregulation. A cell isolation and dilution strategy yielded polyclonal MYC ( myca ) knockout cell pools with low genetic variability and high gene editing efficiencies (as high as 98.2 %). Further isolation and dilution of cells from these pools yielded a monoclonal myca ko cell line harboring a 1-bp deletion that caused a frameshift mutation. This frameshift functionally inactivated the transcriptional regulatory and DNA-binding domains predicted by bioinformatics and structural analyses. Both the polyclonal and monoclonal myca ko cell lines were viable, propagated well in standard medium, and differed from wild-type cells in morphology. As such, they represent a new tool for causally linking myca to cellular osmoregulation and other cell functions.