Study of tissue morphology and vascular endothelial growth factor and matrix metalloproteinase-9 gene expressions in nude mouse endometriosis model

王丹波, 张淑兰, 牛慧彦, 陆景明
In: Chin J Obstet Gynecol · 2004 · vol. 39(11) , pp. 759–762 · doi:10.3760/j.issn:0529-567x.2004.11.011 · W3031994539
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This study established a nude mouse endometriosis model, finding that ectopic endometrial implants adhered, vascularized, and exhibited increased VEGF and MMP-9 gene expression compared to in-situ endometrium.

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This study established a nude mouse model of endometriosis by implanting human secretory-phase endometrium into the pelvic and abdominal cavities to analyze tissue morphology and gene expression over thirty days. Researchers observed that ectopic lesions developed robust blood supply and fused with host tissues, while RT-PCR analysis revealed significantly enhanced mRNA expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) in the ectopic sites compared to eutopic endometrium. The findings indicated that hormone receptor positivity decreased as the lesions matured, confirming that the nude mouse model accurately replicates the biological behavior and progression of human endometriosis. This paper is centrally about endometriosis — specifically focusing on the molecular mechanisms and histological development within an experimental animal model of the disease.

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Abstract

目的 建立子宫内膜异位症(内异症)裸鼠模型,并对其相关生物学行为进行研究.方法取人分泌晚期子宫内膜,剪碎,置入裸鼠盆、腹腔不同部位,内异症(EM组)和非内异症(NEM组)裸鼠各24只;对照组:3只裸鼠,同法将人大网膜组织置入裸鼠盆、腹腔不同部位.各组裸鼠分别于置入后第5、15、30天随机脱颈椎处死,观察异位病灶生长情况,并留取异位病灶,在光镜及透射电镜下观察其形态学变化.RT-PCR法检测在位内膜及异位病灶血管内皮生长因子(VEGF)、基质金属蛋白酶9(MMP-9)mRNA表达,免疫组化链霉素抗生物素蛋白-过氧化物酶连接法检测异位病灶雌、孕激素受体表达.结果内膜置入5 d,即见异位病灶牢固黏附,血液供应丰富.光镜下见腺体细胞及散在间质细胞,且随时间延长,异位病灶与裸鼠组织融合越明显.15 d时,电镜下可见异位病灶内炎性细胞增生、浸润最明显,30 d时腺细胞中细胞器消失,但仍可见分泌颗粒,核染色质聚集,并有凋亡趋势.与在位内膜比较,异位病灶VEGF、MMP-9 mRNA表达增强(P 0.05).结论裸鼠作为内异症研究的动物模型,科学、简便、可行,其异位病灶发生、发展过程与人类内异症相似,且内异症是多因素相关性疾病。
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人子宫内膜异位症裸鼠模型组织形态学变化及血管内皮生长因子和基质金属蛋白酶9基因表达的研究 Study of tissue morphology and vascular endothelial growth factor and matrix metalloproteinase-9 gene expressions in nude mouse endometriosis model 摘要目的建立子宫内膜异位症(内异症)裸鼠模型,并对其相关生物学行为进行研究.方法取人分泌晚期子宫内膜,剪碎,置入裸鼠盆、腹腔不同部位,内异症(EM组)和非内异症(NEM组)裸鼠各24只;对照组:3只裸鼠,同法将人大网膜组织置入裸鼠盆、腹腔不同部位.各组裸鼠分别于置入后第5、15、30天随机脱颈椎处死,观察异位病灶生长情况,并留取异位病灶,在光镜及透射电镜下观察其形态学变化.RT-PCR法检测在位内膜及异位病灶血管内皮生长因子(VEGF)、基质金属蛋白酶9(MMP-9)mRNA表达,免疫组化链霉素抗生物素蛋白-过氧化物酶连接法检测异位病灶雌、孕激素受体表达.结果内膜置入5 d,即见异位病灶牢固黏附,血液供应丰富.光镜下见腺体细胞及散在间质细胞,且随时间延长,异位病灶与裸鼠组织融合越明显.15 d时,电镜下可见异位病灶内炎性细胞增生、浸润最明显,30 d时腺细胞中细胞器消失,但仍可见分泌颗粒,核染色质聚集,并有凋亡趋势.与在位内膜比较,异位病灶VEGF、MMP-9 mRNA表达增强(P<0.05),异位内膜置入第15 天时表达最强(VEGF:EM组为1.11±0.13、NEM组为1.10±0.11;MMP-9:EM组为1.00±0.11、NEM组为0.99±0.08),第30天时有所下降(VEGF:EM组为0.85±0.11、NEM组为0.86±0.14、MMP-9:EM组为0.77±0.13、 NEM组为0.76±0.11).置入晚期(第30天)异位病灶中雌、孕激素受体表达阳性率(雌激素受体表达阳性率: EM组为4/8、NEM组为5/8;孕激素受体表达阳性率:EM组为4/8、NEM组为4/8)低于置入早期(第5天,雌激素受体表达阳性率:EM组为7/8、NEM组为7/8;孕激素受体表达阳性率:EM组为8/8、NEM组为7/8)、中期(第15天,雌激素受体表达阳性率:EM组为7/8、NEM组为7/8;孕激素受体表达阳性率:EM组为7/8、NEM组为8/8),且多数病灶呈弱阳性表达.EM组和NEM组异位病灶中VEGF、MMP-9 mRNA的表达及雌、孕激素受体表达情况比较,差异无显著性(P>0.05).结论裸鼠作为内异症研究的动物模型,科学、简便、可行,其异位病灶发生、发展过程与人类内异症相似,且内异症是多因素相关性疾病. 更多相关知识 - 浏览391 - 被引21 - 下载148 相似文献 - 中文期刊 - 外文期刊 - 学位论文 - 会议论文

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