Cordycepin derived from engineered Pichia pastoris inhibits fibrosarcoma HT1080 cells by targeting Akt1 and kinase activity

Cordycepin derived from engineered Pichia pastoris inhibits fibrosarcoma HT1080 cells by targeting Akt1 and kinase activity | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 17 January 2025 V1 Latest version Share on Cordycepin derived from engineered Pichia pastoris inhibits fibrosarcoma HT1080 cells by targeting Akt1 and kinase activity Authors : Xin Qiu , Chenyang Li , Jing Wang , Hongyou Yu , Renjun Wang , Liang Wang , Shiqi Zhang , Yu Tang , Zihui Li , and Qian Li 0000-0001-8340-5035 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.173710998.89396227/v1 238 views 148 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Fibrosarcoma cells exhibit low sensitivity to chemotherapy and significant drug resistance, emphasizing the urgent need for effective, low-toxicity therapeutic agents with reliable production methods and novel treatment strategies. Cordycepin (3’-deoxyadenosine) has shown promising therapeutic potential in cancer treatment. In this study, cordycepin was produced using a genetically engineered Pichia pastoris strain cultured in an inorganic salt medium and purified to over 98% purity via macroporous resin chromatography, providing a cost-effective production alternative. The effects of cordycepin on the human fibrosarcoma cell line HT1080 were assessed using microscopic examination, scratch assays, CCK-8 assays, and flow cytometry (Annexin V-FITC/PI staining). The results demonstrated that cordycepin significantly inhibited cell activity at an effective concentration of 100 μmol/L. Key observations included changes in cell morphology, reduced migration, inhibited proliferation, cell cycle arrest at the G0/G1 and G2/M phases, and induction of apoptosis. Network pharmacology analysis identified 31 potential targets of cordycepin in fibrosarcoma, with its effects on Akt1 (protein kinase B) and disruption of protein phosphorylation pathways emerging as key mechanisms underlying its therapeutic efficacy. Supplementary Material File (figures.docx) Download 1.38 MB File (manuscript.docx) Download 54.16 KB Information & Authors Information Version history V1 Version 1 17 January 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords akt1 cordycepin human fibrosarcoma ht1080 cells kinase activity network pharmacology Authors Affiliations Xin Qiu Dalian University View all articles by this author Chenyang Li Dalian University View all articles by this author Jing Wang Dalian University View all articles by this author Hongyou Yu Dalian University View all articles by this author Renjun Wang Dalian University View all articles by this author Liang Wang Dalian Polytechnic University View all articles by this author Shiqi Zhang Dalian University View all articles by this author Yu Tang Dalian University View all articles by this author Zihui Li Dalian University View all articles by this author Qian Li 0000-0001-8340-5035 [email protected] Dalian University View all articles by this author Metrics & Citations Metrics Article Usage 238 views 148 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Xin Qiu, Chenyang Li, Jing Wang, et al. Cordycepin derived from engineered Pichia pastoris inhibits fibrosarcoma HT1080 cells by targeting Akt1 and kinase activity. Authorea . 17 January 2025. DOI: https://doi.org/10.22541/au.173710998.89396227/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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