Neural substrates related to memory consolidation of learning multiple motor sequences during wakeful rest

Neural substrates related to memory consolidation of learning multiple motor sequences during wakeful rest | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Neural substrates related to memory consolidation of learning multiple motor sequences during wakeful rest Sungshin Kim, Seojin Yoon, Antoine Caraballo This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7048121/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Motor learning has been thought to be independent of the hippocampus since the landmark experiment of the patient H.M. However, recent studies have provided cumulative evidence of the hippocampus's role in motor memory consolidation during both sleep and wakeful rest. Motor memory consolidation due to hippocampal plastic changes could occur faster than previously thought, even during short wakeful rest periods interleaved with practice periods. However, it remains unclear to what extent hippocampal replay or reactivation contributes to motor memory consolidation during wakeful rest. We investigated the fMRI evidence of motor memory consolidation of learning multiple motor sequence during wakeful rest. We found that while sensorimotor regions exhibited sequence-specific activity patterns during practice, these were not reliably discriminable in the sensorimotor regions and hippocampus during brief interleaved rest periods. In contrast, neural activity patterns in the right hippocampus, right putamen, and sensorimotor regions during interleaved rest periods showed greater similarity to post-learning rest patterns than pre-learning rest patterns. These findings suggest that hippocampal reactivation may not be related to a specific sequence as previously claimed, but rather to a more generalized or abstract representation of sequences, potentially to stabilize and integrate newly acquired skills when learning multiple sequences simultaneously. Biological sciences/Neuroscience/Learning and memory/Consolidation Biological sciences/Psychology/Human behaviour Biological sciences/Neuroscience/Motor control Biological sciences/Neuroscience/Sensorimotor processing Full Text Additional Declarations There is NO Competing Interest. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7048121","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":486555939,"identity":"dbd84c6f-e7a9-4192-a2f0-41a933ab74e5","order_by":0,"name":"Sungshin Kim","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABFklEQVRIiWNgGAWjYLACxgYJBglmBsbHyIIGxGhhNiZFCwODBAMDmzRRWuRn5B5++XOHRZ5kO3dadUHNncT+2e0XGH7UMBibN2DXYnAjL82a94xEsTQz77bbM449S5xx50wBY88xBjOZAzi0SOSYGTO2SSTOA2nhYTuc2HAjJ4GBt4HBRgKnw3LMDH9CtRTz/DucOB+ohfEvHi0MN3KMH/ACtcwGamHmbTucuOFG+gFmoC1muLQYnHljxgzSMrOZd7M0b99h4403chgOyxyTMMbpsPYc448/2+oSZ5w/u/Ezz7fDsvNupD98+KbGxnAGLocBowPFOMcGBh6DA+B4wg2YPyDz7BkY2B/gUz4KRsEoGAUjDwAA5zFbz4SmS/cAAAAASUVORK5CYII=","orcid":"https://orcid.org/0000-0001-9500-4677","institution":"Hanyang University","correspondingAuthor":true,"prefix":"","firstName":"Sungshin","middleName":"","lastName":"Kim","suffix":""},{"id":486555940,"identity":"a65efcbe-6c8b-4f93-b465-33dee0ef2b93","order_by":1,"name":"Seojin Yoon","email":"","orcid":"https://orcid.org/0009-0002-6974-1344","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Seojin","middleName":"","lastName":"Yoon","suffix":""},{"id":486555941,"identity":"b792a31e-0b0c-4395-a96f-7bb4bb85e664","order_by":2,"name":"Antoine Caraballo","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Antoine","middleName":"","lastName":"Caraballo","suffix":""}],"badges":[],"createdAt":"2025-07-04 15:25:08","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-7048121/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-7048121/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":101520237,"identity":"05610c90-74b6-4fcf-94ae-349c16079764","added_by":"auto","created_at":"2026-01-30 17:00:52","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1739090,"visible":true,"origin":"","legend":"Article File","description":"","filename":"HPReplayYoonCaraSubmittedCommBiol.pdf","url":"https://assets-eu.researchsquare.com/files/rs-7048121/v1_covered_5389444f-5852-481a-8a17-6cf52df72485.pdf"}],"financialInterests":"There is \u003cb\u003eNO\u003c/b\u003e Competing Interest.","formattedTitle":"Neural substrates related to memory consolidation of learning multiple motor sequences during wakeful rest","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-7048121/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-7048121/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Motor learning has been thought to be independent of the hippocampus since the landmark experiment of the patient H.M. 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