Study on the correlation between polymorphism of migration inhibitory factor gene promoter region-794CATT microsatellite and endometriosis

Objective:To explore the correlation between polymorphism of migration inhibitory factor(MIF) gene promoter region-794CATT microsatellite and hereditary susceptibility of endometriosis. Methods:Polymerase chain reaction(PCR) and DNA sequencing technique were used to detect the polymorphism of MIF gene promoter region-794CATT microsatellite in 43 cases with endometriosis(endometriosis group) and 44 cases without endometriosis(control group) of Han nationality in Guangdong,MIF genotyping was carried out. Results:The distribution frequencies of MIF gene promoter region-794CATT genotypes(5/5,5/6,5/7,6/6,7/7) in endometriosis group were 51.2%,11.6%,4.7%,30.2% and 2.3%,respectively;the distribution frequencies of MIF gene promoter region-794CATT genotypes(5/5,5/6,5/7,6/6,6/7,7/7) in control group were 25.0%,11.4%,2.3%,50.0%,4.5% and 6.8%,respectively,the distribution frequency of MIF gene promoter region-794CATT 5/5 genotype in endometriosis group was significantly higher than that in control group(P=0.015,OR=3.143,95%CI=1.281～7.713);the distribution frequencies of MIF gene promoter region-794CATT alleles(5,6,7) in endometriosis group were 59.3%,36.0% and 4.7%,respectively;the distribution frequencies of MIF gene promoter region-794CATT alleles(5,6,7) in control group were 31.8%,58.0% and 10.2%,respectively;the distribution frequency of MIF gene promoter region-794CATT(5) allelotype in endometriosis group was significantly higher than that in control group(P=0.038,OR=3.122,95%CI=1.688～5.774). Conclusion:The polymorphism of MIF gene promoter region-794CATT microsatellite is related to hereditary susceptibility of endometriosis among Han population in Guangdong,MIF-794CATT(5/5) genotype and MIF-794CATT(5) allelotype may be the susceptible genotypes.