Abstract
Resolution of sister chromatid intertwines in mitosis is vital for safeguarding genome integrity. While cohesin, condensin, the polo-like kinase Cdc5, and the phosphatase Cdc14 have all been implicated in this process, the underlying molecular mechanisms remain elusive. Our study unveils a coordination between spindle elongation and the timely resolution of DNA linkages, orchestrated by Cdc14 and Cdc5. We show that Cdc14 and Cdc5 collaborate to facilitate the resolution of DNA catenanes, the predominant species of DNA intertwines in mitosis, by regulating Topoisomerase II (Top2) function, both through SUMOylation and phosphorylation. Our findings contribute to unraveling a mechanism wherein Cdc5 and Cdc14 work together to ensure the timely removal of all sources of linkages between sister chromatids, thereby preserving the integrity of chromosome segregation and genome stability.
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Abstract
Resolution of sister chromatid intertwines in mitosis is vital for safeguarding genome integrity. While cohesin, condensin, the polo-like kinase Cdc5, and the phosphatase Cdc14 have all been implicated in this process, the underlying molecular mechanisms remain elusive. Our study unveils a coordination between spindle elongation and the timely resolution of DNA linkages, orchestrated by Cdc14 and Cdc5. We show that Cdc14 and Cdc5 collaborate to facilitate the resolution of DNA catenanes, the predominant species of DNA intertwines in mitosis, by regulating Topoisomerase II (Top2) function, both through SUMOylation and phosphorylation. Our findings contribute to unraveling a mechanism wherein Cdc5 and Cdc14 work together to ensure the timely removal of all sources of linkages between sister chromatids, thereby preserving the integrity of chromosome segregation and genome stability.
Competing Interest Statement
The authors have declared no competing interest.
Data Availability
Data are available in the main text or the supplementary materials. The Chip-seq data have been deposited to the GEO repository (GSE294907). The following secure token has been created to allow review of record GSE294907 while it remains in private status: sdebyikwhzqnvqn
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