Optimization of High-Performance Liquid Chromatography (HPLC) Method for the Detection of Phthalate Acid Esters (PAEs)

Optimization of High-Performance Liquid Chromatography (HPLC) Method for the Detection of Phthalate Acid Esters (PAEs) | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Optimization of High-Performance Liquid Chromatography (HPLC) Method for the Detection of Phthalate Acid Esters (PAEs) ZEBA SADIA This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7965616/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Phthalate acid esters (PAEs) are endocrine disruptors and significant environmental pollutants that are frequently used as plasticizers in consumer and industrial products. Sensitive, repeatable analytical techniques are necessary for the accurate detection of PAEs in environmental matrices. Four important PAEs were quantitatively determined in this study using an optimized high-performance liquid chromatography (HPLC) method in conjunction with a UV-Vis detector: dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), and di(2-ethylhexyl) phthalate (DEHP). Excellent separation was accomplished in 20 minutes using a Shimadzu LC-20 AD system with a C18 column and a gradient mode 90:10 acetonitrile–water mobile phase. At 4.3, 5.7, 13.5, and 15.7 minutes, respectively, retention times were reported. In addition to 20 µL offering the best peak resolution, the technique showed excellent linearity (R2 > 0.99), accuracy, and repeatability across a range of injection volumes. For routine monitoring of PAEs in environmental water and sediment samples, this easy-to-use, quick, and affordable HPLC-UV technique is an efficient substitution for more complicated chromatographic techniques like GC-MS or HPLC-MS/MS. Analytical Chemistry Environmental Chemistry Phthalate Acid Esters UV–Visible Detection (UV–Vis) Gradient Elution Method Optimization Retention Time Chromatographic Separation. Full Text Additional Declarations The authors declare potential competing interests as follows: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Supplementary Files suplementryfilehplc.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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