Thermally Adaptive Surface Microscopy for brain functional imaging

Thermally Adaptive Surface Microscopy for brain functional imaging | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Thermally Adaptive Surface Microscopy for brain functional imaging Hadrien M.L. Robert, Giulia Faini, Chang Liu, Anis Aggoun, Elena Putti, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6395993/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Fluorescence microscopes can capture the dynamics of living cells with high spatio-temporal resolution in a single plane. However, monitoring rapid and dim fluorescence fluctuations, e.g. induced by neuronal activity in the brain, remains challenging for 3D-distributed emitters due to out-of-focus fluorescence background, a restricted photon budget, and the speed limit of conventional scanning systems. Here, we introduce a Thermally Adaptive Surface strategy, allowing the parallel imaging of 3D-distributed objects at speeds only limited by the camera framerate or photon budget. This microscope add-on leverages on an array of thermally tuneable microlenses that offers low chromatic aberration and high transmission, and can be combined with patterned illumination to provide optical sectioning. We demonstrate its potential in vivo, by simultaneously monitoring fast fluorescent dynamics at different depths in the zebrafish larval brain, at 0.5 kHz rate within a 360x360x90 µm^3 observable volume. Fluorescence imaging Ca2+ imaging 3D microscopy electro-thermal modulator Full Text Additional Declarations The authors declare no competing interests. Supplementary Files SupplementaryThermallyAdaptiveSurfaceMicroscopyforbrainfunctionalimaging.pdf Supplementary Information : Thermally Adaptive Surface Microscopy for brain functional imaging Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6395993","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":439660116,"identity":"76b478f3-b24d-43d8-97de-6987e363f89a","order_by":0,"name":"Hadrien M.L. Robert","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA9klEQVRIiWNgGAWjYBACAxBKADN5QIQND1RCjlgtCWkwLcb4tTAgtByGSeDWYs5+eNuHBxV3GMzbew8+rvxxXsac//CxTzcYDPJxabHsSSuekXDmGYPMmXPJhmcSbvNYzkhLnp3DYGDZgMthB3KMGRLbDjNISOSYSTYAtRjc4DFmzmH4Y4BDB4PB+TdALf/AWsx/NiSc4zE4f/4zUIsBbi03QLY0QGxhbEg4wAO0l5mAlmfFDAnHDvNI8JxLlmxISwY6LA3oMAM8Ws4nb2b8UXNYToK99+DHBhs7e4Pzhx8z51Tg1gIDPGh8ghpGwSgYBaNgFOADANkxUVFP1CUwAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0002-6185-3379","institution":"Institut de la Vision","correspondingAuthor":true,"prefix":"","firstName":"Hadrien","middleName":"M.L.","lastName":"Robert","suffix":""},{"id":439660117,"identity":"e57653c0-d2d9-45e1-944f-009a2cbe4896","order_by":1,"name":"Giulia Faini","email":"","orcid":"https://orcid.org/0000-0002-8029-6205","institution":"Institut de la Vision","correspondingAuthor":false,"prefix":"","firstName":"Giulia","middleName":"","lastName":"Faini","suffix":""},{"id":439660118,"identity":"1c197285-074f-4c03-a890-baeba76631aa","order_by":2,"name":"Chang Liu","email":"","orcid":"","institution":"Institut de la Vision","correspondingAuthor":false,"prefix":"","firstName":"Chang","middleName":"","lastName":"Liu","suffix":""},{"id":439660119,"identity":"542bd5a9-5adb-4ac3-b5de-59c6ff09b7e3","order_by":3,"name":"Anis Aggoun","email":"","orcid":"","institution":"Institut de la Vision","correspondingAuthor":false,"prefix":"","firstName":"Anis","middleName":"","lastName":"Aggoun","suffix":""},{"id":439660120,"identity":"24f564eb-3b4e-45d6-9238-2bbf2e9962f3","order_by":4,"name":"Elena Putti","email":"","orcid":"","institution":"Institut de la Vision","correspondingAuthor":false,"prefix":"","firstName":"Elena","middleName":"","lastName":"Putti","suffix":""},{"id":439660121,"identity":"5ff82ebe-2e45-4839-ab9f-31c91132400d","order_by":5,"name":"Jose Garcia-Guirado","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Jose","middleName":"","lastName":"Garcia-Guirado","suffix":""},{"id":439660122,"identity":"634c193b-6c54-4537-a6bb-0614b4556ac8","order_by":6,"name":"Filippo Del Bene","email":"","orcid":"","institution":"Institut de la Vision","correspondingAuthor":false,"prefix":"","firstName":"Filippo","middleName":"Del","lastName":"Bene","suffix":""},{"id":439660123,"identity":"f3b8f69e-62b0-49d6-afe0-31b713aa433f","order_by":7,"name":"Romain Quidant","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Romain","middleName":"","lastName":"Quidant","suffix":""},{"id":439660124,"identity":"e5722ffa-1186-417d-9cdb-18829d85b4e1","order_by":8,"name":"Gilles Tessier","email":"","orcid":"","institution":"Institut de la Vision","correspondingAuthor":false,"prefix":"","firstName":"Gilles","middleName":"","lastName":"Tessier","suffix":""},{"id":439660125,"identity":"f03d729e-97a1-47cd-a061-cb51f8dc17eb","order_by":9,"name":"Pascal Berto","email":"data:image/png;base64,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","orcid":"https://orcid.org/0000-0003-1664-257X","institution":"Institut de la Vision","correspondingAuthor":true,"prefix":"","firstName":"Pascal","middleName":"","lastName":"Berto","suffix":""}],"badges":[],"createdAt":"2025-04-07 16:03:42","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":true,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":true},"doi":"10.21203/rs.3.rs-6395993/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-6395993/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":80210259,"identity":"46eca5b2-33d3-4fdf-8469-f8978b8a6649","added_by":"auto","created_at":"2025-04-09 08:38:31","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1150288,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6395993/v1_covered_51905a5e-cfaa-42d7-ad3e-3a99eae4e230.pdf"},{"id":80209471,"identity":"057f2abd-3f5b-4f78-bd9e-6386a3b80f61","added_by":"auto","created_at":"2025-04-09 08:30:30","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":1362766,"visible":true,"origin":"","legend":"\u003cp\u003eSupplementary Information : Thermally Adaptive Surface Microscopy for brain functional imaging\u003c/p\u003e","description":"","filename":"SupplementaryThermallyAdaptiveSurfaceMicroscopyforbrainfunctionalimaging.pdf","url":"https://assets-eu.researchsquare.com/files/rs-6395993/v1/fc802e9201eea8cbc528c431.pdf"}],"financialInterests":"The authors declare no competing interests.","formattedTitle":"\u003cp\u003e\u003cstrong\u003eThermally Adaptive Surface Microscopy for brain functional imaging\u003c/strong\u003e\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"Institut de la Vision","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Fluorescence imaging, Ca2+ imaging, 3D microscopy, electro-thermal modulator","lastPublishedDoi":"10.21203/rs.3.rs-6395993/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-6395993/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eFluorescence microscopes can capture the dynamics of living cells with \u0026nbsp;high spatio-temporal resolution in a single plane. However, monitoring \u0026nbsp;rapid and dim fluorescence fluctuations, e.g. induced by neuronal \u0026nbsp;activity in the brain, remains challenging for 3D-distributed emitters \u0026nbsp;due to out-of-focus fluorescence background, a restricted photon budget, \u0026nbsp;and the speed limit of conventional scanning systems. Here, we \u0026nbsp;introduce a Thermally Adaptive Surface strategy, allowing the parallel \u0026nbsp;imaging of 3D-distributed objects at speeds only limited by the camera \u0026nbsp;framerate or photon budget. This microscope add-on leverages on an array \u0026nbsp;of thermally tuneable microlenses that offers low chromatic aberration \u0026nbsp;and high transmission, and can be combined with patterned illumination \u0026nbsp;to provide optical sectioning. We demonstrate its potential in vivo, by \u0026nbsp;simultaneously monitoring fast fluorescent dynamics at different depths \u0026nbsp;in the zebrafish larval brain, at 0.5 kHz rate within a 360x360x90 µm^3 \u0026nbsp;observable volume.\u003c/p\u003e","manuscriptTitle":"Thermally Adaptive Surface Microscopy for brain functional imaging","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-04-09 08:30:25","doi":"10.21203/rs.3.rs-6395993/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"12a01124-6ee2-40bf-aaf1-4366e5eb6691","owner":[],"postedDate":"April 9th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2025-04-11T15:36:32+00:00","versionOfRecord":[],"versionCreatedAt":"2025-04-09 08:30:25","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-6395993","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-6395993","identity":"rs-6395993","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}